Polypeptide nanocomposite as well as preparation method and application thereof

Abstract

The invention discloses a polypeptide nano-composite and a preparation method and application thereof, and belongs to the field of new biological materials, the polypeptide nano-composite comprises a component A and a component B, the component A is polypeptide infiltration liquid; the component B is colloid; the component B is prepared from the following components: a methyl methacrylate grafted and modified SIS (Styrene Isoprene Styrene) hot-melt pressure-sensitive adhesive and modified nano magnesium aluminum silicate-TG enzyme compound particles; the modified nano magnesium aluminum silicate comprises the following components: aluminum-magnesium silicate sol, a template agent, a silane coupling agent, calcium amino acid and glycidyl methacrylate, the addition amount of the silane coupling agent is 1-2% of the mass of the aluminum-magnesium silicate sol, the addition amount of the template agent is 1-2% of the mass of the aluminum-magnesium silicate sol, the addition amount of the calcium amino acid is 3% of the mass of the aluminum-magnesium silicate sol, and the addition amount of the glycidyl methacrylate is 1-2% of the mass of the aluminum-magnesium silicate sol. The addition amount of the glycidyl methacrylate is 10% of the mass of the magnesium aluminum silicate sol. According to the invention, by improving the pressure-sensitive adhesive, the adhesive which still has relatively good viscosity under the infiltration action of polypeptide liquid is obtained.

Description

Technical Field

[0001] The present invention belongs to the field of new biological materials, and relates to a polypeptide nanocomposite, a preparation method thereof, and an application thereof. Background Art

[0002] At present, in the processing field of moisturizing products, polypeptides are often used as active substances to achieve a moisturizing effect. The moisturizing effect of polypeptide substances is good, but they also have a good lubricating effect, which will reduce the adhesion strength between the adhesive layer of the moisturizing product (such as a moisturizing wet wipe) and the skin, making the moisturizing product unable to adhere to the skin for a long time and failing to achieve a good moisturizing effect. Summary of the Invention

[0003] The purpose of the present invention is to provide a polypeptide nanocomposite, a preparation method thereof, and an application thereof, which solve the problem of poor adhesion of existing polypeptide moisturizing products.

[0004] The technical solution adopted by the present invention is as follows:

[0005] A polypeptide nanocomposite includes component A and component B; component A is a tackifier for component B; component A is a polypeptide infiltration solution; component B is a colloid;

[0006] Component B includes the following components: SIS hot melt pressure-sensitive adhesive grafted with methyl methacrylate, modified nanoaluminosilicate magnesium - TG enzyme complex particles;

[0007] The modified nanoaluminosilicate magnesium includes the following components: magnesium aluminosilicate sol, template agent, silane coupling agent, calcium amino acid, glycidyl methacrylate. The addition amount of the silane coupling agent is 1 - 2% of the mass of the magnesium aluminosilicate sol, the addition amount of the template agent is 1 - 2% of the mass of the magnesium aluminosilicate sol, the addition amount of calcium amino acid is 3% of the mass of the magnesium aluminosilicate sol, and the addition amount of glycidyl methacrylate is 10% of the mass of the magnesium aluminosilicate sol.

[0008] The present invention designs a polypeptide nanocomposite capable of preparing moisturizing products, such as moisturizing wet wipes, moisturizing masks, etc. Component A, the polypeptide infiltration solution in the present invention, plays a moisturizing role, and component B plays an adhesion role; the present invention improves the pressure-sensitive adhesive to prepare a peelable adhesive that still has good adhesion under the infiltration of component A, and the peelable adhesive is component B.

[0009] The component B of the present invention uses SIS hot-melt pressure-sensitive adhesive grafted with methyl methacrylate and modified nano magnesium aluminum silicate-TG enzyme complex particles as main adhesive components. When the component B is mixed with the component A, the modified nano magnesium aluminum silicate in the modified nano magnesium aluminum silicate-TG enzyme complex particles will swell in water to form a colloid, which not only has certain adhesiveness but also forms more active sites. After the expansion, the layered structure of the magnesium aluminum silicate is stretched open, and the active sites originally wrapped inside are exposed on the surface, forming more active sites. These sites can adsorb polypeptide molecules, so that the polypeptide molecules contact the TG enzyme on the magnesium aluminum silicate colloid and undergo a cross-linking reaction, and a cross-linking network structure is formed between the polypeptide molecules to generate stable polypeptide adhesive sites, so that the component B has a strong bonding strength with the skin. Compared with the prior art, the influence of the component A on the adhesiveness of the overall product is reduced.

[0010] The template agent in the present invention is used to regulate the porous structure of nano-magnesium aluminum silicate, and the silane coupling agent, amino acid calcium and glycidyl methacrylate are all modifiers of nano-magnesium aluminum silicate. The present invention sequentially uses the silane coupling agent, glycidyl methacrylate and amino acid calcium to modify the magnesium aluminum silicate particles. The modification of the silane coupling agent is conducive to the modification of the magnesium aluminum silicate particles by glycidyl methacrylate. The modification of the magnesium aluminum silicate particles by glycidyl methacrylate can simultaneously enhance the bonding strength between the magnesium aluminum silicate particles and the TG enzyme and the SIS hot-melt pressure-sensitive adhesive grafted and modified by methyl methacrylate, thereby avoiding the aggregation of the TG enzyme. The introduction of amino acid calcium further improves the catalytic efficiency of the TG enzyme and promotes the cross-linking reaction between polypeptide molecules. The silane coupling agent, glycidyl methacrylate and amino acid calcium interact with each other, so that in the presence of component A, component B has better integrity, stability and viscosity.

[0011] Furthermore, the polypeptide impregnation solution includes the following components: squalene, sunflower seed oil, jojoba oil, sorbitan stearate, palmitoyl tetrapeptide-7, nonapeptide-1, acetyl peptide, and bee venom peptide.

[0012] The polypeptide impregnation liquid and TG enzyme in the present invention work synergistically to significantly enhance the adhesion of the wet wipes and provide a good moisturizing effect.

[0013] Furthermore, the amount of the modified nano-magnesium aluminum silicate-TG enzyme complex particles added is 6-8% of the mass of the SIS hot melt pressure sensitive adhesive grafted with methyl methacrylate.

[0014] In the present invention, the SIS hot melt pressure-sensitive adhesive modified by grafting of methyl methacrylate is the main adhesive substance, and its viscosity is better than the viscosity of the polypeptide cross-linked product and the viscosity of the magnesium aluminum silicate after swelling. Therefore, the present invention optimizes the ratio of the modified nano magnesium aluminum silicate-TG enzyme complex particles and the SIS hot melt pressure-sensitive adhesive modified by grafting of methyl methacrylate.

[0015] Furthermore, the modified nanoaluminum magnesium silicate - TG enzyme complex particles are prepared by the following method:

[0016] B. Preparation of modified nanoaluminum magnesium silicate: Prepare magnesium aluminum silicate sol with tetraethyl orthosilicate, hydrochloric acid, aluminum - magnesium mixed solution, and ammonia water as the main components. Then add a template agent to the magnesium aluminum silicate sol, and obtain porous nano - magnesium aluminum silicate particles through stirring, standing, aging, drying, and calcination. Then, successively modify the porous nano - magnesium aluminum silicate particles with a silane coupling agent, glycidyl methacrylate, and calcium amino acid to obtain modified nanoaluminum magnesium silicate particles loaded with calcium amino acid;

[0017] B. Disperse the modified nanoaluminum magnesium silicate particles in water to obtain a suspension, then add TG enzyme particles to the modified nanoaluminum magnesium silicate suspension, stir for 1 - 2 hours, add glutaraldehyde, continue to stir and react at 45°C for 1 hour, then wash and dry to obtain modified nanoaluminum magnesium silicate - TG enzyme complex particles.

[0018] Furthermore, the aluminum - magnesium mixed solution contains aluminum chloride and magnesium chloride with a mass ratio of 3:1.

[0019] Furthermore, the mass ratio of the modified nanoaluminum magnesium silicate particles to the TG enzyme particles is 3:1.

[0020] Furthermore, the methyl methacrylate - grafted modified SIS hot - melt pressure - sensitive adhesive is prepared by the following method: While stirring, drop a mixed solution of methyl methacrylate and BPO initiator into the molten SIS hot - melt pressure - sensitive adhesive. The mass ratio of methyl methacrylate to BPO initiator in the mixed solution is 20:1, and the addition amount of methyl methacrylate is 5% of the mass of the SIS hot - melt pressure - sensitive adhesive. React at 80°C for 3 hours, then continue to add BPO initiator. At this time, the addition amount of BPO initiator is half of the first addition amount of BPO initiator. After stirring evenly, continue to react for 1 hour. Then cool down to 50°C, and then add terpene resin. After stirring evenly, obtain the methyl methacrylate - grafted modified SIS hot - melt pressure - sensitive adhesive.

[0021] The preparation method of the polypeptide nano - complex described above includes the following steps:

[0022] S1. Preparation of Component A: In a container, heat deionized water to 40 - 50 °C, then add sorbitan stearate. After stirring evenly, sequentially add palmitoyl tetrapeptide - 7, nonapeptide - 1, acetylated peptide, and melittin, and stir evenly to obtain an aqueous solution containing active ingredients. In another container, mix squalane, sunflower seed oil, and jojoba oil to obtain an oil mixture. Then slowly add the oil mixture to the aqueous solution containing active ingredients while continuously stirring, and use a homogenizer to promote the emulsification process until a stable emulsion is formed. This emulsion is Component A.

[0023] S2. Preparation of Component B: Respectively prepare methyl methacrylate - grafted modified SIS hot - melt pressure - sensitive adhesive and modified nano - aluminum magnesium silicate - TG enzyme complex particles. Add the modified nano - aluminum magnesium silicate - TG enzyme complex particles to the molten methyl methacrylate - grafted modified SIS hot - melt pressure - sensitive adhesive at 40 °C, and stir evenly to obtain Component B.

[0024] The application of the polypeptide nanocomposite is used to prepare a moisturizing wet wipe. The moisturizing wet wipe includes cotton soft non - woven fabric, Component A, and Component B. Component B is evenly coated on the dry cotton soft non - woven fabric to form an adhesive layer. After covering the release paper on the adhesive layer of the cotton soft non - woven fabric, it is independently packaged. Component A is independently packaged.

[0025] Further, the moisturizing wet wipe is used by the following method: Immerse the cotton soft non - woven fabric with the adhesive layer in Component A, take it out after soaking for 20 - 30 minutes and then use it.

[0026] Before immersion, the release paper of the cotton soft non - woven fabric with the adhesive layer in the present invention needs to be torn off. After the cotton soft non - woven fabric with the adhesive layer is immersed in the polypeptide soaking solution, the cotton soft non - woven fabric contains polypeptide moisturizing components, and its adhesive layer still has strong adhesiveness. It is not easy to fall off after being applied, and with the progress of the polypeptide cross - linking reaction, the adhesiveness retention time is longer.

[0027] In summary, due to the adoption of the above - mentioned technical solutions, the beneficial effects of the present invention are as follows:

[0028] 1. The present invention utilizes the expansibility of modified nano - aluminum magnesium silicate and the catalytic cross - linking effect of TG enzyme on polypeptides to solve the problem of weak adhesiveness in polypeptide moisturizing products.

[0029] 2. In the present invention, an interaction occurs among the silane coupling agent, glycidyl methacrylate, and calcium amino acid. In the presence of Component A, Component B has good integrity, stability, and adhesiveness.

[0030] 3. The moisturizing wet wipe in the present invention includes dry and wet independent packaging, which avoids the premature cross - linking reaction between TG enzyme and polypeptide components and is also conducive to the separate storage of dry and wet components. Description of the Drawings

[0031] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the embodiments. It should be understood that the following drawings only show some embodiments of the present invention, and thus should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings, where:

[0032] Figure 1 It is a structural schematic diagram of the moisturizing wet wipe in Embodiment 1;

[0033] 1 - Cotton soft non-woven fabric, 2 - Adhesive layer. Specific embodiments

[0034] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention, that is, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Usually, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0035] Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed present invention, but only represents the selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the scope of protection of the present invention.

[0036] It should be noted that relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. Without further limitations, an element defined by the statement "including one..." does not exclude the existence of additional identical elements in the process, method, article or device including the said element.

[0037] The features and performance of the present invention will be further described in detail below in combination with the embodiments.

[0038] Embodiment 1

[0039] A polypeptide nanocomposite provided by a preferred embodiment of the present invention includes component A and component B; component A is a thickening agent for component B; component A is a polypeptide infiltration solution; component B is a colloid.

[0040] Component B includes the following components: SIS hot melt pressure-sensitive adhesive grafted with methyl methacrylate, modified nanoaluminosilicate magnesium-TG enzyme composite particles, and the addition amount of the modified nanoaluminosilicate magnesium-TG enzyme composite particles is 6% of the mass of the SIS hot melt pressure-sensitive adhesive grafted with methyl methacrylate.

[0041] The modified nanoaluminosilicate magnesium includes the following components: aluminosilicate magnesium sol, template agent, silane coupling agent, calcium amino acid, glycidyl methacrylate, the addition amount of the silane coupling agent is 1-2% of the mass of the aluminosilicate magnesium sol, the addition amount of the template agent is 1-2% of the mass of the aluminosilicate magnesium sol, the addition amount of calcium amino acid is 3% of the mass of the aluminosilicate magnesium sol, and the addition amount of glycidyl methacrylate is 10% of the mass of the aluminosilicate magnesium sol; the template agent is cetyltrimethylammonium bromide or polyethylene glycol; the silane coupling agent is KH-550 silane coupling agent.

[0042] Specifically, the modified nanoaluminosilicate magnesium-TG enzyme composite particles are prepared by the following method:

[0043] A. Preparation of modified nanoaluminosilicate magnesium: Mix 10 parts by weight of tetraethyl orthosilicate, 0.3 parts by weight of hydrochloric acid with a concentration of 4 mol / L, and 20 parts by weight of water, stir for 15-16 min to form a gel, add 20 parts by weight of an aluminum-magnesium mixture to the gel, the aluminum-magnesium mixture contains aluminum chloride and magnesium chloride with a mass ratio of 3:1, stir and mix at 40°C and 200 revolutions per minute for 15 min, then dropwise add 8 parts by weight of ammonia water with a concentration of 25 wt% under stirring to obtain aluminosilicate magnesium sol. Add a template agent to the aluminosilicate magnesium sol, stir for 2-3 hours and then stand for 8-10 hours, then age at 40°C for 24 hours, then dry at 60°C for 3-5 hours, and calcine at 500-600°C for 2-3 hours after drying to obtain porous nanoaluminosilicate magnesium particles.

[0044] Add the silane coupling agent to ethanol and stir evenly, then add the porous nanoaluminosilicate magnesium particles, stir for 1-2 hours, filter, wash, and dry to obtain silanized modified aluminosilicate magnesium particles.

[0045] Glycidyl methacrylate was dissolved in tetrahydrofuran to prepare a glycidyl methacrylate solution. Benzoyl peroxide accounting for 0.1% of the mass of glycidyl methacrylate was added. After stirring evenly, the silylated modified magnesium aluminum silicate particles were added to the glycidyl methacrylate solution. The mixture was stirred and reacted at 60 - 80 °C for 2 - 3 hours. After the reaction ended, it was filtered and washed with ethanol, and then dried at 60 °C to obtain glycidyl methacrylate grafted modified magnesium aluminum silicate particles; Calcium amino acid was dissolved in phosphate buffer solution to obtain a calcium amino acid solution. Then, the glycidyl methacrylate grafted modified magnesium aluminum silicate particles were added to the calcium amino acid solution and stirred for 2 - 3 hours. After filtration, it was dried at 60 °C to obtain modified nano magnesium aluminum silicate particles loaded with calcium amino acid;

[0046] B. The modified nano magnesium aluminum silicate particles were dispersed in water to obtain a suspension. Then, the TG enzyme particles were added to the modified nano magnesium aluminum silicate suspension. After stirring for 1 - 2 hours, glutaraldehyde accounting for 0.5% of the mass of the reaction system was added. The mixture was continuously stirred and reacted at 45 °C for 1 hour, then washed and dried to obtain modified nano magnesium aluminum silicate - TG enzyme composite particles, where the mass ratio of the modified nano magnesium aluminum silicate particles to the TG enzyme particles was 3:1.

[0047] Specifically, the methyl methacrylate grafted modified SIS hot melt pressure - sensitive adhesive was prepared by the following method: A mixed solution of methyl methacrylate and BPO initiator was added dropwise with stirring to the molten SIS hot melt pressure - sensitive adhesive. The mass ratio of methyl methacrylate to BPO initiator in the mixed solution was 20:1, and the addition amount of methyl methacrylate was 5% of the mass of the SIS hot melt pressure - sensitive adhesive. After reacting at 80 °C for 3 hours, BPO initiator was continuously added. At this time, the addition amount of BPO initiator was half of the first addition amount of BPO initiator. After stirring evenly, the reaction continued for 1 hour. Subsequently, the temperature was lowered to 50 °C, and then terpene resin was added. After stirring evenly, the methyl methacrylate grafted modified SIS hot melt pressure - sensitive adhesive was obtained.

[0048] Component B was prepared by the following method: The modified nano magnesium aluminum silicate - TG enzyme composite particles were added to the molten methyl methacrylate grafted modified SIS hot melt pressure - sensitive adhesive at 40 °C. After stirring evenly, component B was obtained.

[0049] The polypeptide infiltration solution consists of the following components: squalene, sunflower seed oil, jojoba oil, sorbitan stearate, palmitoyl tetrapeptide-7, nonapeptide-1, acetylated peptide, melittin, and deionized water. The mass fraction of squalene in the polypeptide infiltration solution is 5%, the mass fraction of sunflower seed oil is 5%, the mass fraction of jojoba oil is 5%, the mass fraction of sorbitan stearate is 1%, the mass fraction of palmitoyl tetrapeptide-7 is 0.5%, the mass fraction of nonapeptide-1 is 0.2%, the mass fraction of acetylated peptide is 0.2%, the mass fraction of melittin is 0.1%, and the balance is deionized water.

[0050] The preparation method of the peptide infiltration solution is as follows: In a container, heat the deionized water to 40-50 °C, then add sorbitan stearate, and after stirring evenly, add palmitoyl tetrapeptide-7, nonapeptide-1, acetylated peptide, and melittin in sequence, and stir evenly to obtain an aqueous solution containing active ingredients; In another container, mix squalene, sunflower seed oil, and jojoba oil to obtain an oil mixture; Then slowly add the oil mixture to the aqueous solution containing active ingredients, while continuously stirring, and use a homogenizer to promote the emulsification process until a stable emulsion is formed, and the emulsion is Component A.

[0051] A specific application of this example is: to prepare a moisturizing wet wipe, as Figure 1 shown, the moisturizing wet wipe includes a cotton soft non-woven fabric 1, Component A, and Component B; Component B is evenly coated along the edge of the cotton soft non-woven fabric on the dry cotton soft non-woven fabric to form an adhesive layer 2, and after covering the release paper on the adhesive layer of the cotton soft non-woven fabric, it is independently packaged; Component A is independently packaged; The moisturizing wet wipe is used by the following method: Immerse the cotton soft non-woven fabric with the adhesive layer in Component A, completely submerge the cotton soft non-woven fabric in Component A, take it out after soaking for 20-30 minutes to obtain the moisturizing wet wipe. The size of the cotton soft non-woven fabric is adjusted according to the part to be used.

[0052] Example 2

[0053] Based on Example 1, the difference in this example from Example 1 is: In this example, the addition amount of the modified nanoaluminum magnesium silicate-TG enzyme complex particles is 7% of the mass of the methyl methacrylate-grafted SIS hot melt pressure-sensitive adhesive.

[0054] Example 3

[0055] Based on Example 1, the difference in this example from Example 1 is: In this example, the addition amount of the modified nanoaluminum magnesium silicate-TG enzyme complex particles is 8% of the mass of the methyl methacrylate-grafted SIS hot melt pressure-sensitive adhesive.

[0056] Excessive addition of the modified nano - aluminum magnesium silicate - TG enzyme complex particles will not only bring an obvious sticky feeling to the use of wet wipes, but also cause an obvious pulling feeling on the skin when peeling the wet wipes. Therefore, the maximum addition amount of the modified nano - aluminum magnesium silicate - TG enzyme complex particles is limited in the present invention.

[0057] Comparative Example 1

[0058] Based on Example 1, the difference between this comparative example and Example 1 is that: the modified nano - aluminum magnesium silicate - TG enzyme complex particles are not added in Component B of this comparative example.

[0059] Comparative Example 2

[0060] Based on Example 1, the difference between this comparative example and Example 1 is that: the hot - melt pressure - sensitive adhesive in Component B of this comparative example is an SIS hot - melt pressure - sensitive adhesive without graft modification.

[0061] Comparative Example 3

[0062] Based on Example 1, the difference between this comparative example and Example 1 is that: in this comparative example, the modified nano - aluminum magnesium silicate is not modified with glycidyl methacrylate.

[0063] Comparative Example 4

[0064] Based on Example 1, the difference between this comparative example and Example 1 is that: in this comparative example, the modified nano - aluminum magnesium silicate is not modified with calcium amino acid.

[0065] Comparative Example 5

[0066] Based on Example 1, the difference between this comparative example and Example 1 is that: in this comparative example, the modified nano - aluminum magnesium silicate is not modified with silane coupling agent.

[0067] Comparative Example 6

[0068] Based on Example 1, the difference between this comparative example and Example 1 is that: the mass ratio of the modified nano - aluminum magnesium silicate particles to the TG enzyme particles is 4:1.

[0069] Comparative Example 7

[0070] Based on Example 1, the difference between this comparative example and Example 1 is that: the mass ratio of the modified nano - aluminum magnesium silicate particles to the TG enzyme particles is 2:1.

[0071] Test Example 1

[0072] The holding time of the stickiness of the wet wipes was judged by detecting the peeling strength of the moisturizing wet wipes prepared in Examples 1 - 3 and Comparative Examples 1 - 7. The detection method is the prior art, and the detection results are shown in Table 1.

[0073] The method for detecting the peeling strength is as follows: measure the force required to peel the moisturizing wet wipe from the steel plate (the application angle is 180 degrees, and the peeling speed is 270 mm / min to 330 mm / min);

[0074] The method for detecting the adhesive retention time is as follows: respectively detect the initial peeling strength (Q0) of the moisturizing wet wipe on the steel plate and the peeling strengths after adhesion for 5 minutes, 10 minutes, 20 minutes, and 30 minutes. The method for detecting the peeling strength refers to the above method. The peeling strength after adhesion for 5 minutes is denoted as Q1, the peeling strength after adhesion for 10 minutes is denoted as Q2, the peeling strength after adhesion for 20 minutes is denoted as Q3, and the peeling strength after adhesion for 30 minutes is denoted as Q4.

[0075] Table 1 Detection of the peeling strength and adhesiveness of the moisturizing wet wipe

[0076]

[0077]

[0078] In the present invention, if the proportion of the modified nanoaluminum magnesium silicate particles is too large, it will hinder the catalytic dosage of TG enzyme after swelling; if the proportion of the modified nanoaluminum magnesium silicate particles is too small, the thickening effect is not obvious. When the peeling strength is greater than 2 N / cm, there will be an obvious pulling feeling on the skin, causing discomfort. The TG enzyme-catalyzed polypeptide cross-linking in the present invention is a process. As the cross-linking reaction proceeds, the adhesiveness will first increase and will not continue to increase after the cross-linking reaction ends. During the process of applying the wet wipe, when the cross-linking reaction ends, the adhesiveness of the moisturizing wet wipe will gradually decrease; compared with the existing moisturizing wet wipes, the adhesiveness of the moisturizing wet wipe in the present invention first increases and then decreases, while the prior art gradually decreases. Therefore, the adhesiveness duration of the moisturizing wet wipe in the present invention is long. If the peeling strength of the moisturizing wet wipe is less than 0.5 N / cm, it is easy to fall off, and after it is less than 0.1 N / cm, there is basically no obvious adhesiveness. The adhesive retention time of the present invention is greater than 30 minutes.

[0079] The above are only the preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent replacements, and improvements made by those skilled in the art within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A polypeptide nanocomplex, characterized in that: It comprises component A and component B; the component A is a viscosity enhancer for component B; the component A is a polypeptide infiltration liquid; the component B is a colloid; The component B comprises the following components: SIS hot melt pressure sensitive adhesive grafted with methyl methacrylate, and modified nano magnesium aluminum silicate-TG enzyme complex particles; The modified nano magnesium aluminum silicate comprises the following components: magnesium aluminum silicate sol, a template agent, a silane coupling agent, amino acid calcium, and glycidyl methacrylate, wherein the amount of the silane coupling agent added is 1-2% of the mass of the magnesium aluminum silicate sol, the amount of the template agent added is 1-2% of the mass of the magnesium aluminum silicate sol, the amount of the amino acid calcium added is 3% of the mass of the magnesium aluminum silicate sol, and the amount of the glycidyl methacrylate added is 10% of the mass of the magnesium aluminum silicate sol.

2. The polypeptide nanocomplex according to claim 1, characterized in that: The polypeptide soaking solution comprises the following components: squalene, sunflower seed oil, jojoba oil, sorbitan stearate, palmitoyl tetrapeptide-7, nonapeptide-1, acetyl peptide and bee venom peptide.

3. A polypeptide nanocomplex according to claim 1, characterized in that: The amount of the modified nano-magnesium aluminum silicate-TG enzyme complex particles added is 6-8% of the mass of the SIS hot melt pressure sensitive adhesive grafted with methyl methacrylate.

4. A polypeptide nanocomplex according to claim 2, characterized in that: The modified nano magnesium aluminum silicate-TG enzyme complex particles are prepared by the following method: A. Preparation of modified nano magnesium aluminum silicate: preparing magnesium aluminum silicate sol with tetraethyl orthosilicate, hydrochloric acid, aluminum magnesium mixed solution and ammonia water as main components, adding a template agent to the magnesium aluminum silicate sol, and obtaining porous nano magnesium aluminum silicate particles by stirring, standing, aging, drying and calcining; and modifying the porous nano magnesium aluminum silicate particles with silane coupling agent, glycidyl methacrylate and amino acid calcium in turn to obtain modified nano magnesium aluminum silicate particles loaded with amino acid calcium; B. Disperse the modified nano-magnesium aluminum silicate particles in water to obtain a suspension, then add the TG enzyme particles to the modified nano-magnesium aluminum silicate suspension, stir for 1-2 hours, then add glutaraldehyde, continue stirring and reacting at 45° C. for 1 hour, then wash and dry to obtain the modified nano-magnesium aluminum silicate-TG enzyme complex particles.

5. A polypeptide nanocomplex according to claim 4, characterized in that: The aluminum-magnesium mixed liquid contains aluminum chloride and magnesium chloride in a mass ratio of 3:

1.

6. The polypeptide nanocomplex according to claim 1, characterized in that: The mass ratio of modified nano-magnesium aluminum silicate particles to TG enzyme particles is 3:

1.

7. The polypeptide nanocomplex according to claim 1, characterized in that: The SIS hot melt pressure sensitive adhesive modified by grafting of methyl methacrylate is prepared by the following method: a mixed solution of methyl methacrylate and BPO initiator is added dropwise into the molten SIS hot melt pressure sensitive adhesive while stirring, the mass ratio of methyl methacrylate to BPO initiator in the mixed solution is 20:1, the amount of methyl methacrylate added is 5% of the mass of the SIS hot melt pressure sensitive adhesive, and the BPO initiator is continuously added after reacting at 80° C. for 3 hours, at which time the amount of BPO initiator added is half of the amount of the first BPO initiator added, and the reaction is continued for 1 hour after stirring evenly, and then the temperature is lowered to 50° C., and terpene resin is added, and the mixture is stirred evenly to obtain the SIS hot melt pressure sensitive adhesive modified by grafting of methyl methacrylate.

8. A method for preparing a polypeptide nanocomplex according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. Prepare component A: in one container, heat deionized water to 40-50° C., add sorbitan stearate, stir evenly, then add palmitoyl tetrapeptide-7, nonapeptide-1, acetyl peptide and bee venom peptide in sequence, stir evenly to obtain an aqueous solution containing active ingredients; in another container, mix squalene, sunflower seed oil and jojoba oil to obtain a fat mixture; then slowly add the fat mixture to the aqueous solution containing the active ingredient, while continuously stirring, and use a homogenizer to promote the emulsification process until a stable emulsion is formed, and the emulsion is component A; S2. Preparation of component B: prepare SIS hot melt pressure sensitive adhesive modified by grafting of methyl methacrylate and modified nano magnesium aluminum silicate-TG enzyme complex particles respectively, add modified nano magnesium aluminum silicate-TG enzyme complex particles to SIS hot melt pressure sensitive adhesive modified by grafting of methyl methacrylate at 40° C., stir evenly and obtain component B.

9. The use of a polypeptide nanocomplex according to any one of claims 1 to 7, characterized in that: Used to prepare a moisturizing wet wipe, the moisturizing wet wipe includes a cotton non-woven fabric, component A and component B; component B is evenly coated on the dry cotton non-woven fabric to form a circle of sticky layer, the sticky layer of the cotton non-woven fabric is covered with release paper and then independently packaged; component A is independently packaged.

10. The use of a polypeptide nanocomplex according to claim 9, characterized in that: The moisturizing wet wipes are used in the following method: soaking the cotton non-woven fabric with the sticky layer in component A, and taking it out after soaking for 20-30 minutes for use.

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