Pressure type micro-fluidic chip and detection equipment thereof

Abstract

The invention relates to the technical field of nucleic acid detection, in particular to a pressure type micro-fluidic chip and detection equipment thereof, the micro-fluidic chip comprises a chip body, the chip body is provided with a sample adding hole, a detection pool, a plurality of reaction pools, a plurality of reagent pools and a plurality of circulation grooves, the plurality of reaction pools are communicated in sequence, and the plurality of reagent pools are communicated in sequence. The plurality of reaction pools are located between the sample adding hole and the detection pool, the reagent pools are correspondingly arranged around the reaction pools, meanwhile, the openings between the adjacent reaction pools are communicated through the circulation grooves, and meanwhile, the openings of the reagent pools are communicated with the openings of the corresponding reaction pools. Solutions in the reaction tank and the reagent tank are extruded by the pressure hammer, so that all the solutions in the reaction tank flow through the circulating groove. The reaction tank has the effect of reducing solution residues in the reaction tank.

Description

Technical Field

[0001] The present application relates to the technical field of nucleic acid detection, and in particular, to a pressure microfluidic chip and its detection device. Background Art

[0002] A microfluidic chip, also known as a microfluidics chip, is a technology that integrates small-scale liquid fluid processes on a small chip. This chip is usually made of glass or plastic, on which tiny channels and chambers are engraved, enabling precise manipulation of the flow and mixing of liquids in a tiny space. Microfluidic chips are widely used in the fields of biomedicine, chemical analysis, environmental monitoring, etc., because they can achieve high-efficiency and high-sensitivity experiments while reducing the required reagent and sample volumes.

[0003] Currently, a patent with a publication number of CN111607506B discloses a thin-film nucleic acid amplification chip body and its preparation and application methods. The thin-film nucleic acid amplification chip body includes multiple reaction chambers connected by channels; the reaction chambers include a lysis chamber, a purification chamber, a pre-amplification chamber, and an amplification chamber; the lysis chamber is provided with a sample inlet and is connected to the purification chamber; the purification chamber is respectively connected to a first liquid storage chamber pre-filled with a nucleic acid purification solution and a second liquid storage chamber pre-filled with a nucleic acid elution solution; the purification chamber is pre-encapsulated with silica-coated magnetic beads and is connected to the pre-amplification chamber; the pre-amplification chamber is connected to a third liquid storage chamber pre-filled with a nucleic acid amplification dilution solution and is connected to the amplification chamber.

[0004] In view of the above related technologies, the flow of the solution between each channel relies on the gravity of the solution itself. Therefore, each channel usually flows in from the top side of the reaction chamber and out from the bottom side. However, the microfluidic chip is small and each channel is thin, and there will be a small amount of solution at the bottom of the reaction chamber that is difficult to flow out through the channel by gravity. Thus, each solution is likely to remain in the reaction chamber. Summary of the Invention

[0005] In order to reduce the residue of the solution in each reaction chamber, improve the solution volume accuracy, accelerate the liquid flow, enhance the sealing effect to prevent contamination, and reduce the production cost related to the chip body, the present application provides a pressure microfluidic chip and its detection device.

[0006] In a first aspect, a pressure microfluidic chip provided by the present application adopts the following technical solution: A pressure - type microfluidic chip, including a chip body. There is a cavity inside the chip body. The chip body is provided with a sample injection hole, a detection pool, a plurality of reaction pools, a plurality of reagent pools and a plurality of flow channels. The plurality of reaction pools include a sample lysis pool, a magnetic bead reaction pool and an amplification mixing pool that are connected in sequence. The sample injection hole is connected to the sample lysis pool, the detection pool is connected to the amplification mixing pool. There are magnetic beads at the bottom of the magnetic bead reaction pool. A drainage groove is also provided on one side of the opening of the magnetic bead reaction pool, and the drainage groove is connected to the cavity inside the chip body. The plurality of reagent pools include a washing solution pool, an elution solution pool, an amplification solution pool and a primer - probe pool. The washing solution pool and the elution solution pool are respectively connected to the magnetic bead reaction pool, and the amplification solution pool and the primer - probe pool are respectively connected to the amplification mixing pool. The plurality of flow channels are used to connect the openings between adjacent reaction pools, and also used to connect the openings of the reagent pools to the openings of the corresponding reaction pools.

[0007] By adopting the above - mentioned technical solution, during the experiment, the sample solution is injected into the sample lysis pool through the sample injection hole. After the sample is lysed in the sample lysis pool, the detection device will use a pressure hammer to squeeze the solution in the sample lysis pool. The pressure hammer above the lysis pool will squeeze all the solution in the lysis pool through the flow channel into the magnetic bead reaction pool. After the magnetic beads are adsorbed, the pressure hammer is used to squeeze all the solution in the washing solution pool through the flow channel into the magnetic bead reaction pool. After washing, the magnetic beads are adsorbed by a magnet, and then the pressure hammer is used again to squeeze all the solution in the magnetic bead reaction pool through the drainage groove into the cavity inside the chip body for drainage.

[0008] Then the pressure hammer above the magnetic bead reaction pool resets, and the pressure hammer above the elution solution pool squeezes all the solution in the magnetic bead reaction pool through the flow channel into the amplification mixing pool. At this time, the pressure hammers above the amplification solution pool and the primer - probe pool respectively squeeze all the solution in the amplification solution pool and the primer - probe pool through the flow channel into the amplification mixing pool. After amplification is completed, the pressure hammer above the amplification mixing pool is used to squeeze all the solution in the amplification mixing pool through the flow channel into the detection pool.

[0009] Using the pressure hammer to squeeze the solution makes all the solution in the reaction pool flow through the flow channel, reducing the solution residue in the reaction pool. At the same time, when the pressure hammer squeezes the solution in the reaction pool, it can make the solution flow more quickly.

[0010] Optionally, the bottoms of the reaction pool and the reagent pool are both spherical, and the concave side faces the opening direction of the reaction pool.

[0011] By adopting the above - mentioned technical solution, the spherical reaction pool and reagent pool are more easily squeezed by the pressure hammer, making the reaction pool and reagent pool drain the solution more smoothly.

[0012] Optionally, drainage grooves are provided on the inner walls of the reaction tank and the reagent tank, and one side of the drainage groove communicates with the flow-through groove on the side of the direction of the solution to flow.

[0013] By adopting the above technical solution, the arrangement of the drainage groove facilitates the solution in the reaction tank and the reagent tank to flow along the drainage groove into the flow-through groove under the extrusion of the pressure hammer, reducing the solution residue in the reaction tank and the reagent tank.

[0014] Optionally, there are multiple drainage grooves, and multiple drainage grooves all communicate with the flow-through groove on the side of the direction of the solution to flow.

[0015] By adopting the above technical solution, the arrangement of multiple drainage grooves enhances the fluidity of the solution in the reaction tank and the reagent tank flowing along the drainage groove into the flow-through groove when the solution is under pressure, thereby further reducing the solution residue in the reaction tank and the reagent tank.

[0016] Optionally, the side of the same drainage groove away from the flow-through groove is located on the same contour plane.

[0017] By adopting the above technical solution, the reaction tank and the reagent tank at each height level can flow towards the flow-through groove when being extruded by the pressure hammer.

[0018] Optionally, the reaction tank further includes a quantitative tank, the quantitative tank is located between the magnetic bead reaction tank and the amplification mixing tank, one side of the quantitative tank communicates with the magnetic bead reaction tank, and the other side communicates with the amplification mixing tank; the reagent tank further includes a residual liquid tank, and the residual liquid tank communicates with the quantitative tank.

[0019] By adopting the above technical solution, with the cooperation of the quantitative tank and the residual liquid tank, after the solution in the magnetic bead reaction tank is transported to the quantitative tank, the overflowing solution will flow into the residual liquid tank, so that the solution transported to the amplification mixing tank in the quantitative tank is a certain amount, making the experimental data more accurate.

[0020] Optionally, the detection tank includes a first detection tank and a second detection tank, and the amplification mixing tank, the first detection tank and the second detection tank are connected in sequence.

[0021] By adopting the above technical solution, by placing solutions at different ambient temperatures in the first detection tank and the second detection tank, it is convenient for the detection personnel to perform PCR detection.

[0022] Optionally, the washing liquid tank includes a first washing liquid tank and a second washing liquid tank, and the first washing liquid tank and the second washing liquid tank are respectively connected to the magnetic bead reaction tank.

[0023] By adopting the above technical solution, with the cooperation of the first washing liquid pool and the second washing liquid pool, when the reaction is carried out in the magnetic bead reaction pool, the sample adsorbed by the magnetic beads in the magnetic bead reaction pool can be washed twice.

[0024] In a second aspect, a detection device provided by the present application adopts the following technical solution: A detection device includes the above pressure microfluidic chip; a lower clamping plate, on which a limiting groove is provided for receiving the chip body; a magnet rotatably connected to the lower clamping plate to move away from or close to the bottom of the magnetic bead reaction pool; a heating element fixedly connected to the lower clamping plate and located at the bottom of the detection pool; an upper clamping plate located above the lower clamping plate, on which a plurality of pressure hammers, a plurality of pressure valves and a driving member for driving the pressure hammers and the pressure valves to move are installed; the pressure hammers are respectively arranged corresponding to the reaction pool, the reagent pool and the detection pool, and the shape of the pressure hammers is consistent with the inner walls of the corresponding reaction pool, reagent pool and detection pool; the pressure valves are respectively arranged corresponding to the flow-through groove and the liquid discharge groove, and the pressure valves are used to close or open the corresponding flow-through groove or liquid discharge groove.

[0025] By adopting the above technical solution, during the process of nucleic acid detection, the chip body is placed in the limiting groove, and then the sample solution is added into the sample lysis pool through the sample adding hole.

[0026] After the sample is lysed, the driving member drives the pressure hammer located above the sample lysis pool to extrude the solution in the sample lysis pool, and at the same time the driving member opens the pressure valve between the sample lysis pool and the magnetic bead reaction pool, so that all the solution in the sample lysis pool is transferred into the magnetic bead reaction pool, and then the driving member closes the pressure valve between the sample lysis pool and the magnetic bead reaction pool again.

[0027] After the nucleic acid in the solution in the magnetic bead reaction pool is adsorbed by the magnetic beads, the driving member drives the pressure hammer located above the washing liquid pool to extrude the washing liquid pool, and at the same time the driving member opens the pressure valve between the washing liquid pool and the magnetic bead reaction pool, so that all the solution in the washing liquid pool is transferred into the magnetic bead reaction pool, and then the driving member closes the pressure valve between the washing liquid pool and the magnetic bead reaction pool again.

[0028] After the washing is completed, the magnet is moved close to the bottom of the magnetic bead reaction pool to adsorb the magnetic beads by the magnet. Then the driving member drives the pressure hammer located above the magnetic bead reaction pool to extrude the solution in the magnetic bead reaction pool, and at the same time the driving member opens the pressure valve above the liquid discharge groove, so that the pressure hammer discharges all the solution in the magnetic bead reaction pool through the liquid discharge groove. Then the driving member closes the pressure valve above the liquid discharge groove again, and at the same time the driving member drives the pressure hammer located above the magnetic bead reaction pool to reset.

[0029] Then, move the magnet away from the bottom of the magnetic bead reaction pool. The driving member drives the pressure hammer located above the eluent pool to squeeze the solution in the eluent pool. At the same time, the driving member opens the pressure valve between the eluent pool and the magnetic bead reaction pool, so that the pressure hammer transfers all the solution in the eluent pool to the magnetic bead reaction pool. Then, the driving member closes the pressure valve between the eluent pool and the magnetic bead reaction pool.

[0030] After the nucleic acid is detached from the magnetic beads, the driving member drives the pressure hammer located above the magnetic bead reaction pool to squeeze the magnetic bead reaction pool. At the same time, the driving member opens the pressure valve between the magnetic bead reaction pool and the amplification mixing pool, so that all the solution in the magnetic bead reaction pool is transferred to the amplification mixing pool. Then, the driving member closes the pressure valve between the magnetic bead reaction pool and the amplification mixing pool.

[0031] Then, the driving member drives the pressure hammer located above the amplification solution pool to squeeze the amplification solution pool. At the same time, the driving member opens the pressure valve between the amplification solution pool and the amplification mixing pool, so that all the solution in the amplification solution pool is transferred to the amplification mixing pool. Then, the driving member closes the pressure valve between the amplification solution pool and the amplification mixing pool.

[0032] Next, the driving member drives the pressure hammer located above the primer-probe pool to squeeze the primer-probe pool. At the same time, the driving member opens the pressure valve between the primer-probe pool and the amplification mixing pool, so that all the solution in the primer-probe pool is transferred to the amplification mixing pool. Then, the driving member closes the pressure valve between the primer-probe pool and the amplification mixing pool.

[0033] After nucleic acid amplification, the driving member drives the pressure hammer located above the amplification solution pool to squeeze the amplification mixing pool. At the same time, the driving member opens the pressure valve between the amplification mixing pool and the detection pool, so that all the solution in the amplification solution pool is transferred to the detection pool. Then, the driving member closes the pressure valve between the amplification mixing pool and the detection pool.

[0034] Using the pressure hammer to squeeze the solution makes all the solution in the reaction pool flow through the flow channel, accelerating the flow rate of the solution and making the experimental process faster.

[0035] Optionally, the lower clamping plate is further equipped with an ultrasonic device, and the ultrasonic device is located at the bottom of the sample lysis pool.

[0036] By adopting the above technical solution, the setting of the ultrasonic device facilitates the solution in the sample lysis pool.

[0037] In summary, the present application includes at least one of the following beneficial technical effects: 1. By providing a drain groove, the internal space of the chip body is fully utilized to accommodate the washing liquid, avoiding the need to additionally open a space for accommodating the washing liquid on the chip body. Moreover, the accommodating space is larger, the structure is simpler, and at the same time, the area of the chip body can be reduced, and the production cost can be lowered.

[0038] 2. Through the cooperation of the chip body, reaction chamber, reagent chamber, and flow channel, the solution is extruded by the pressure hammer, enabling all the solution in the reaction chamber to flow through the flow channel, reducing the solution residue in the reaction chamber. At the same time, when the pressure hammer extrudes the solution in the reaction chamber, it can make the solution flow more rapidly. 3. Through the setting of the drainage channel, it is convenient for the solution in the reaction chamber and reagent chamber to flow along the drainage channel into the flow channel under the extrusion of the pressure hammer, reducing the solution residue in the reaction chamber and reagent chamber. 4. Through the cooperation of the metering chamber and the residual liquid chamber, after the solution in the magnetic bead reaction chamber is transported to the metering chamber, the overflow solution will flow into the residual liquid chamber. Thus, the solution transported to the amplification mixing chamber in the metering chamber is a fixed quantity, making the experimental data more accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 is the overall structural schematic diagram of the pressure microfluidic chip in Embodiment 1 of the present application.

[0040] Figure 2 is the top view structural schematic diagram of the pressure microfluidic chip in Embodiment 1 of the present application.

[0041] Figure 3 is the overall structural schematic diagram of the detection device in Embodiment 1 of the present application.

[0042] Figure 4 is the cross-sectional structural schematic diagram of the lower clamping plate in Embodiment 1 of the present application.

[0043] Figure 5 is the overall structural schematic diagram of the detection device in Embodiment 2 of the present application.

[0044] Figure 6 is the overall structural schematic diagram of the limiting component in Embodiment 2 of the present application.

[0045] Figure 7 is the cross-sectional structural schematic diagram of the limiting component in Embodiment 2 of the present application.

[0046] Figure 8 is the cross-sectional structural schematic diagram of the mounting seat in Embodiment 2 of the present application.

[0047] Explanation of the reference numerals: 1. chip body; 2. sample loading hole; 3. detection pool; 31. first detection pool; 32. second detection pool; 4. reaction pool; 41. sample lysis pool; 42. magnetic bead reaction pool; 43. quantitative pool; 44. amplification mixing pool; 5. drainage trough; 6. reagent pool; 61. washing liquid pool; 611. first washing liquid pool; 612. second washing liquid pool; 62. elution liquid pool; 63. residual liquid pool; 64. amplification liquid pool; 65. primer probe pool; 7. circulation trough; 8. drainage trough; 10. lower clamp; 101. limit groove; 102. magnet; 103. electric heating element; 104. ultrasonic device; 20. upper clamp; 201. pressure hammer; 202. pressure valve; 203. driving element; 30. Mounting seat; 40, limit assembly; 401, mounting body; 4011, mounting cavity; 4012, mounting support rod; 402, mounting hole; 403, abutment; 4031, limit block; 4032, sliding hole; 4033, guide surface; 4034, first spring; 404, manual drive member; 4041, drive column; 4042, drive hole; 4043, second spring; 405, conversion member; 4051, intermediate rod; 4052, first long hole; 4053, first column; 4054, second long hole; 4055, second column; 50, pop-up assembly; 501, pop-up plate; 502, pop-up slot; 503, pop-up spring; 60, mounting assembly; 601, mounting bolt; 602, mounting long hole; DETAILED DESCRIPTION The following is combined with Figure 1-8 This application is described in further detail.

[0048] Example 1: This example of the application discloses a pressure-type microfluidic chip.

[0049] Reference Figure 1 and Figure 2 A pressure-type microfluidic chip includes a chip body 1, a plurality of reaction pools 4, a plurality of reagent pools 6, and a flow channel 7. The chip body 1 is provided with a sample addition hole 2 and a detection pool 3, the plurality of reaction pools 4 are located between the sample addition hole 2 and the detection pool 3, the reagent pool 6 is arranged around the corresponding reaction pool 4, and the flow channel 7 is provided on the chip body 1, the flow channel 7 connects the openings between adjacent reaction pools 4, and also connects the openings of the reagent pool 6 with the openings of the corresponding reaction pool 4.

[0050] In this embodiment, the bottoms of the reaction pool 4 and the reagent pool 6 are both spherical, and the concave side faces the opening direction of the reaction pool 4. In other embodiments, the bottoms of the reaction pool 4 and the reagent pool 6 can also be set to an inverted triangle or an inverted trapezoid. The spherical reaction pool 4 and the reagent pool 6 are more easily squeezed by the pressure hammer 201, so that the reaction pool 4 and the reagent pool 6 can discharge the solution more smoothly.

[0051] Reference Figure 1and Figure 2 The reaction cell 4 includes a sample lysis cell 41, a magnetic bead reaction cell 42, and an amplification mixing cell 44 that are connected in sequence. The sample lysis cell 41, the magnetic bead reaction cell 42, and the amplification mixing cell 44 are formed on the chip body 1. The sample loading hole 2 is connected to the sample lysis cell 41, and the detection cell 3 is connected to the amplification mixing cell 44. Magnetic beads are provided at the bottom of the magnetic bead reaction cell 42. A cavity is formed inside the chip body 1. A drain groove 5 is further formed on one side of the opening of the magnetic bead reaction cell 42. The drain groove 5 connects the magnetic bead reaction cell 42 to the cavity inside the chip body 1. In this embodiment, by providing the drain groove 5, the internal space of the chip body 1 is fully utilized to accommodate the washing liquid, with a larger accommodation space, a simpler structure, avoiding the need to additionally provide a space for accommodating the washing liquid on the chip body 1, simplifying the structure of the chip body 1, reducing the area of the chip body 1, and lowering the production cost.

[0052] Refer to Figure 1 and Figure 2 The reagent cell 6 includes a washing liquid cell 61, an elution liquid cell 62, an amplification liquid cell 64, and a primer-probe cell 65. The washing liquid cell 61 and the elution liquid cell 62 are respectively connected to the magnetic bead reaction cell 42, and the amplification liquid cell 64 and the primer-probe cell 65 are respectively connected to the amplification mixing cell 44.

[0053] By using the cooperation of the sample lysis cell 41, the magnetic bead reaction cell 42, the amplification mixing cell 44, the washing liquid cell 61, the elution liquid cell 62, the amplification liquid cell 64, and the primer-probe cell 65, the sample solution added through the sample loading hole 2 is reacted and finally transferred to the detection cell 3 for observation.

[0054] Refer to Figure 1 and Figure 2 The washing liquid cell 61 includes a first washing liquid cell 611 and a second washing liquid cell 612. The first washing liquid cell 611 and the second washing liquid cell 612 are respectively connected to the magnetic bead reaction cell 42.

[0055] In this embodiment, by using the cooperation of the first washing liquid cell 611 and the second washing liquid cell 612, when the reaction is carried out in the magnetic bead reaction cell 42, the sample adsorbed by the magnetic beads in the magnetic bead reaction cell 42 can be washed twice. In addition, according to needs, the first washing liquid cell 611 and the second washing liquid cell 612 can accommodate washing liquids with different properties or functions, making the washing more thorough. Moreover, since in this embodiment, the magnetic bead reaction cell 42 is connected to the cavity inside the chip body 1 through the drain groove 5, more washing liquid generated due to multiple washings can be handled.

[0056] Refer to Figure 1 and Figure 2, the reaction tank 4 further includes a metering tank 43, which is located between the magnetic bead reaction tank 42 and the amplification mixing tank 44. One side of the metering tank 43 is communicated with the magnetic bead reaction tank 42, and the other side is communicated with the amplification mixing tank 44. The reagent tank 6 further includes a waste liquid tank 63, which is communicated with the metering tank 43.

[0057] By using the cooperation of the metering tank 43 and the waste liquid tank 63, after the solution in the magnetic bead reaction tank 42 is transported to the metering tank 43, the overflow solution will flow into the waste liquid tank 63. Furthermore, the solution transported from the metering tank 43 to the amplification mixing tank 44 is quantitatively controlled, making the control of the solution volume more precise and the experimental data more accurate.

[0058] Refer to Figure 1 and Figure 2 , the detection tank 3 further includes a first detection tank 31 and a second detection tank 32, and the amplification mixing tank 44, the first detection tank 31, and the second detection tank 32 are connected in sequence. By placing solutions with different ambient temperatures in the first detection tank 31 and the second detection tank 32, the detection efficiency of the detection personnel is greatly improved.

[0059] Refer to Figure 1 and Figure 2 , drainage grooves 8 are opened on the inner walls of both the reaction tank 4 and the reagent tank 6. The arrangement of the drainage grooves 8 facilitates that under the extrusion of the pressure hammer 201, the solution in the reaction tank 4 and the reagent tank 6 can flow along the drainage grooves 8 into the flow-through groove 7, reducing the solution residue in the reaction tank 4 and the reagent tank 6.

[0060] Refer to Figure 1 and Figure 2 , there are multiple drainage grooves 8, and all the multiple drainage grooves 8 are communicated with the flow-through groove 7 on the side of the direction of flow. In this embodiment, three drainage grooves 8 are provided. In other embodiments, two, four or other numbers can be opened adaptively according to the sizes of the reaction tank 4 and the reagent tank 6. One side of all the drainage grooves 8 is communicated with the flow-through groove 7 on the side of the direction of flow, and the sides of the same drainage groove 8 far from the flow-through groove 7 are all located on the same contour plane.

[0061] The arrangement of the multiple drainage grooves 8 further enhances the fluidity of the solution in the reaction tank 4 and the reagent tank 6 flowing along the drainage grooves 8 into the corresponding flow-through groove 7 when the solution is under pressure, thereby further reducing the solution residue in the reaction tank 4 and the reagent tank 6. At the same time, the sides of the drainage grooves 8 far from the flow-through groove 7 being all located on the same contour plane enables the reaction tank 4 and the reagent tank 6 at each height layer to flow towards the flow-through groove 7 under the extrusion of the pressure hammer 201.

[0062] In this embodiment, since the pressure microfluidic chip needs to be provided with pressure by the pressure hammer 201 of the corresponding detection device to realize the flow of the solution in the chip body 1. In order to prevent the solution from overflowing to the upper side of the chip body 1 under the action of the pressure hammer 201, a layer of isolation film is covered on the upper side of the chip body 1, and the isolation film is adhered to the surface of the chip body 1, so that the solution can only flow along the corresponding flow channel 7.

[0063] The implementation principle of a pressure microfluidic chip according to an embodiment of the present application is as follows: During the experiment, the sample solution is injected into the sample lysis pool 41 through the sampling hole 2. After the sample is lysed in the sample lysis pool 41, the detection device will use the pressure hammer 201 to squeeze the solution in the sample lysis pool 41, and squeeze all the solution in the sample lysis pool 41 into the magnetic bead reaction pool 42 through the flow channel 7. After the magnetic beads are adsorbed, the pressure hammer 201 is used to squeeze all the solution in the first washing liquid pool 611 into the magnetic bead reaction pool 42 through the flow channel 7. After washing, the magnetic beads are adsorbed by the magnet 102, and then the pressure hammer 201 is used again to squeeze all the solution in the magnetic bead reaction pool 42 into the cavity in the chip body 1 through the drain groove 5 for drainage. The pressure hammer 201 located above the magnetic bead reaction pool 42 is reset, and then the pressure hammer 201 is used again to squeeze all the solution in the second washing liquid pool 612 into the magnetic bead reaction pool 42 through the flow channel 7. After the second washing, the magnetic beads are adsorbed by the magnet 102, and then the pressure hammer 201 is used again to squeeze all the solution in the magnetic bead reaction pool 42 into the cavity inside the chip body 1 through the drain groove 5 for drainage.

[0064] Then the pressure hammer 201 located above the magnetic bead reaction pool 42 is reset, and the pressure hammer 201 located above the eluent pool 62 squeezes the solution in the eluent pool 62 into the magnetic bead reaction pool 42. After the nucleic acid is separated from the magnetic beads, the pressure hammer 201 above the magnetic bead reaction pool 42 squeezes the magnetic bead reaction pool 42, so that the solution in the magnetic bead reaction pool 42 is squeezed into the quantitative pool 43 through the flow channel 7. When the quantitative pool 43 is full, the excess solution in the quantitative pool 43 flows into the waste liquid pool 63. After all the solution in the magnetic bead reaction pool 42 is squeezed into the quantitative pool 43 through the flow channel 7, the solution in the quantitative pool 43 is still a fixed amount.

[0065] Subsequently, the pressure hammer 201 located above the metering cell 43 squeezes the solution in the metering cell 43 into the amplification mixing cell 44. At this time, the pressure hammers 201 located above the amplification solution cell 64 and the primer-probe cell 65 respectively squeeze all the solutions in the amplification solution cell 64 and the primer-probe cell 65 into the amplification mixing cell 44 through the flow channel 7. After the amplification is completed, the pressure hammer 201 located above the amplification mixing cell 44 squeezes all the solution in the amplification mixing cell 44 into the first detection cell 31 through the flow channel 7. It is also possible to squeeze the detection solution in the first detection cell 31 into the second detection cell 32 through the pressure hammer 201 above the first detection cell 31. At the same time, the detection solution in the second detection cell 32 can also be squeezed back into the first detection cell 31 through the pressure hammer 201 above the second detection cell 32. By using different temperatures in the first detection cell 31 and the second detection cell 32, it is possible to adapt to different types of nucleic acids for isothermal amplification for detection.

[0066] The pressure hammer 201 is used to squeeze the solution, so that all the solutions in the reaction cell 4 flow through the flow channel 7, reducing the solution residue in the reaction cell 4. At the same time, when the pressure hammer 201 squeezes the solution in the reaction cell 4, it can make the solution flow faster. At the same time, there is no need to set up an air circuit, saving the space for designing the air circuit, so that the chip body 1 can be made thinner and lighter.

[0067] This embodiment also discloses a detection device.

[0068] Refer to Figure 3 and Figure 4 A detection device includes a lower clamping plate 10 and an upper clamping plate 20. A limiting groove 101 is formed in the lower clamping plate 10. A magnet 102 and a heating element 103 are arranged in the limiting groove 101. The magnet 102 is rotatably connected to the lower clamping plate 10, and the heating element 103 is fixedly connected to the lower clamping plate 10. The upper clamping plate 20 is located above the lower clamping plate 10. A plurality of pressure hammers 201 and a plurality of pressure valves 202 are installed on the upper clamping plate 20. Driving members 203 are arranged on both the pressure hammer 201 and the pressure valve 202. The driving member 203 is a micro cylinder or a linear module. Driven by the driving member 203, both the pressure hammer 201 and the pressure valve 202 can move in the vertical direction.

[0069] Refer to Figure 3 and Figure 4 The magnet 102 on the lower clamping plate 10 corresponds to the bottom of the magnetic bead reaction cell 42 and can approach or move away from the bottom of the magnetic bead reaction cell 42 to adsorb or release the magnetic beads in the magnetic bead reaction cell 42. In this embodiment, the magnet 102 is rotatably connected to the lower clamping plate 10, and the rotation of the motor is used to control the magnet 102 to rotate towards or away from the bottom of the magnetic bead reaction cell 42.

[0070] Refer toFigure 3 and Figure 4 The electrothermal element 103 corresponds to the bottom of the detection cell 3. There are also two electrothermal elements 103. One electrothermal element 103 is located below the first detection cell 31, and the other electrothermal element 103 is located below the second detection cell 32. The first detection cell 31 and the second detection cell 32 can be maintained at the required temperature by the two electrothermal elements 103.

[0071] Refer to Figure 3 and Figure 4 Multiple pressure hammers 201 are respectively arranged corresponding to the upper parts of the sample lysis cell 41, the magnetic bead reaction cell 42, the quantification cell 43, the waste liquid cell 63, the amplification mixing cell 44, the first detection cell 31, the second detection cell 32, the first washing liquid cell 611, the second washing liquid cell 612, the elution liquid cell 62, the amplification liquid cell 64, and the primer-probe cell 65. Each time the solution is transferred, the pressure hammer 201 can squeeze the corresponding solution. And the shape of the bottom of the pressure hammer 201 is consistent with the inner walls of the corresponding sample lysis cell 41, magnetic bead reaction cell 42, quantification cell 43, waste liquid cell 63, amplification mixing cell 44, first detection cell 31, second detection cell 32, first washing liquid cell 611, second washing liquid cell 612, elution liquid cell 62, amplification liquid cell 64, and primer-probe cell 65. The surface of the pressure hammer 201 is made of rubber material or polymer compound and has a certain elasticity, so that the pressure hammer 201 can squeeze all the solution in each cell onto the flow channel 7 when squeezing.

[0072] Refer to Figure 3 and Figure 4 Multiple pressure valves 202 are respectively arranged corresponding to the upper sides of several flow channels 7 and the drain tank 5, and the pressure valve 202 is in plug-in fit with the corresponding flow channel 7 or drain tank 5. Driven by the corresponding driving member 203, the pressure valve 202 is inserted into the corresponding flow channel 7 or drain tank 5. At this time, the pressure valve 202 closes the corresponding flow channel 7 or the corresponding drain tank 5. Therefore, the setting of the pressure valve 202 can realize the closing or opening of the corresponding flow channel 7 or drain tank 5.

[0073] Refer to Figure 3 and Figure 4 In an optional embodiment, an ultrasonic device 104 is further installed on the lower clamping plate 10, and the ultrasonic device 104 is located at the bottom of the sample lysis cell 41. The setting of the ultrasonic device 104 facilitates the lysis of nucleic acids in the sample lysis cell 41.

[0074] The implementation principle of a detection device according to an embodiment of the present application is as follows: During the process of nucleic acid detection, the chip body 1 is placed in the limiting groove 101, and then the sample solution is added into the sample lysis cell 41 through the sample adding hole 2.

[0075] After the sample is lysed, the driving member 203 drives the pressure hammer 201 located above the sample lysis pool 41 to squeeze the solution in the sample lysis pool 41. At the same time, the driving member 203 opens the pressure valve 202 between the sample lysis pool 41 and the magnetic bead reaction pool 42, so that all the solution in the sample lysis pool 41 is transferred into the magnetic bead reaction pool 42. Then, the driving member 203 closes the pressure valve 202 between the sample lysis pool 41 and the magnetic bead reaction pool 42.

[0076] After the nucleic acid in the solution in the magnetic bead reaction pool 42 is adsorbed by the magnetic beads, the driving member 203 drives the pressure hammer 201 located above the first washing liquid pool 611 to squeeze the first washing liquid pool 611. At the same time, the driving member 203 opens the pressure valve 202 between the first washing liquid pool 611 and the magnetic bead reaction pool 42, so that all the solution in the first washing liquid pool 611 is transferred into the magnetic bead reaction pool 42. Then, the driving member 203 closes the pressure valve 202 between the first washing liquid pool 611 and the magnetic bead reaction pool 42.

[0077] After the first washing is completed, the magnet 102 is brought close to the bottom of the magnetic bead reaction pool 42, and the magnetic beads are adsorbed by using the magnet 102. Then, the driving member 203 drives the pressure hammer 201 located above the magnetic bead reaction pool 42 to squeeze the solution in the magnetic bead reaction pool 42. At the same time, the driving member 203 opens the pressure valve 202 above the drain tank 5, so that the pressure hammer 201 discharges all the solution in the magnetic bead reaction pool 42 through the drain tank 5. Then, the driving member 203 closes the pressure valve 202 above the drain tank 5, and at the same time, the driving member 203 drives the pressure hammer 201 located above the magnetic bead reaction pool 42 to reset.

[0078] Then, the magnet 102 is moved away from the bottom of the magnetic bead reaction pool 42. The driving member 203 drives the pressure hammer 201 located above the second washing liquid pool 612 to squeeze the second washing liquid pool 612. At the same time, the driving member 203 opens the pressure valve 202 between the second washing liquid pool 612 and the magnetic bead reaction pool 42, so that all the solution in the second washing liquid pool 612 is transferred into the magnetic bead reaction pool 42. Then, the driving member 203 closes the pressure valve 202 between the second washing liquid pool 612 and the magnetic bead reaction pool 42 After the second washing is completed, the magnet 102 is brought close to the bottom of the magnetic bead reaction pool 42, and the magnetic beads are adsorbed by using the magnet 102. Then, the driving member 203 drives the pressure hammer 201 located above the magnetic bead reaction pool 42 to squeeze the solution in the magnetic bead reaction pool 42. At the same time, the driving member 203 opens the pressure valve 202 above the drain tank 5, so that the pressure hammer 201 discharges all the solution in the magnetic bead reaction pool 42 through the drain tank 5. Then, the driving member 203 closes the pressure valve 202 above the drain tank 5, and at the same time, the driving member 203 drives the pressure hammer 201 located above the magnetic bead reaction pool 42 to reset.

[0079] Then, move the magnet 102 away from the bottom of the magnetic bead reaction cell 42. The driving member 203 drives the pressure hammer 201 located above the eluent cell 62 to squeeze the solution in the eluent cell 62. At the same time, the driving member 203 opens the pressure valve 202 between the eluent cell 62 and the magnetic bead reaction cell 42, so that the pressure hammer 201 transfers all the solution in the eluent cell 62 into the magnetic bead reaction cell 42. Then, the driving member 203 closes the pressure valve 202 between the eluent cell 62 and the magnetic bead reaction cell 42.

[0080] After the nucleic acid is detached from the magnetic beads, the driving member 203 drives the pressure hammer 201 located above the magnetic bead reaction cell 42 to squeeze the inside of the magnetic bead reaction cell 42. At the same time, the driving member 203 opens the pressure valve 202 between the magnetic bead reaction cell 42 and the quantitative cell 43, and opens the pressure valve 202 between the quantitative cell 43 and the waste liquid cell 63, so that all the solution in the magnetic bead reaction cell 42 is transferred to the quantitative cell 43, and the solution overflowing from the quantitative cell 43 automatically flows into the waste liquid cell 63. Then, the driving member 203 closes the pressure valve 202 between the magnetic bead reaction cell 42 and the quantitative cell 43, and closes the pressure valve 202 between the quantitative cell 43 and the waste liquid cell 63.

[0081] The driving member 203 drives the pressure hammer 201 located above the quantitative cell 43 to squeeze the inside of the quantitative cell 43. At the same time, the driving member 203 opens the pressure valve 202 between the quantitative cell 43 and the amplification mixing cell 44, so that all the solution in the quantitative cell 43 is transferred into the amplification mixing cell 44. Then, the driving member 203 closes the pressure valve 202 between the magnetic bead reaction cell 42 and the amplification mixing cell 44.

[0082] Then, the driving member 203 drives the pressure hammer 201 located above the amplification solution cell 64 to squeeze the amplification solution cell 64. At the same time, the driving member 203 opens the pressure valve 202 between the amplification solution cell 64 and the amplification mixing cell 44, so that all the solution in the amplification solution cell 64 is transferred into the amplification mixing cell 44. Then, the driving member 203 closes the pressure valve 202 between the amplification solution cell 64 and the amplification mixing cell 44.

[0083] Next, the driving member 203 drives the pressure hammer 201 located above the primer-probe cell 65 to squeeze the primer-probe cell 65. At the same time, the driving member 203 opens the pressure valve 202 between the primer-probe cell 65 and the amplification mixing cell 44, so that all the solution in the primer-probe cell 65 is transferred into the amplification mixing cell 44. Then, the driving member 203 closes the pressure valve 202 between the primer-probe cell 65 and the amplification mixing cell 44.

[0084] After nucleic acid amplification, the driving member 203 drives the pressure hammer 201 located above the amplification liquid pool 64 to squeeze the amplification mixing pool 44. At the same time, the driving member 203 opens the pressure valve 202 between the amplification mixing pool 44 and the first detection pool 31, so that all the solution in the amplification mixing pool 44 is transferred into the first detection pool 31. Then, the driving member 203 closes the pressure valve 202 between the amplification mixing pool 44 and the first detection pool 31.

[0085] When it is necessary to transfer into the first detection pool 31, the driving member 203 drives the pressure hammer 201 located above the first detection pool 31 to squeeze the first detection pool 31. At the same time, the driving member 203 opens the pressure valve 202 between the first detection pool 31 and the second detection pool 32, so that all the solution in the first detection pool 31 is transferred into the second detection pool 32. Then, the driving member 203 closes the pressure valve 202 between the first detection pool 31 and the second detection pool 32.

[0086] When it is necessary to transfer the solution in the second detection pool 32 into the first detection pool 31, the driving member 203 drives the pressure hammer 201 located above the second detection pool 32 to squeeze the second detection pool 32. At the same time, the driving member 203 opens the pressure valve 202 between the first detection pool 31 and the second detection pool 32, so that all the solution in the second detection pool 32 is transferred into the first detection pool 31. Then, the driving member 203 closes the pressure valve 202 between the first detection pool 31 and the second detection pool 32.

[0087] The pressure hammer 201 is used to squeeze the solution, so that all the solution in the reaction pool 4 flows through the flow channel 7, which speeds up the flow rate of the solution and makes the experimental process faster.

[0088] Embodiment 2: The embodiment of the present application discloses a detection device. Refer to Figure 5 , the difference between this embodiment and Embodiment 1 is that: an installation seat 30 is provided on the upper side of the lower clamping plate 10, and the lower side of the installation seat 30 is detachably connected to the lower clamping plate 10. The limiting groove 101 is opened on the upper side of the installation seat 30 and penetrates through the installation seat 30 along its own length direction. The chip body 1 is inserted and matched with the limiting groove 101. The length direction of the chip body 1 is arranged along the length direction of the limiting groove 101, and the chip body 1 slides along its own length direction with the inner side wall of the limiting groove 101.

[0089] Refer to Figure 5 and Figure 6The limiting components 40 are arranged on both sides of the limiting groove 101. The limiting components 40 include a mounting body 401. The two inner walls of the limiting groove 101 are provided with mounting holes 402. The mounting body 401 is plugged into and matched with the mounting holes 402. The mounting body 401 is connected to the mounting seat 30 by bolts. The side of the mounting body 401 facing the chip body 1 is flush with the inner wall corresponding to the limiting groove 101. The matching arrangement of the mounting body 401 and the mounting hole 402 facilitates the installation and removal of the limiting component 40.

[0090] Reference Figure 5 and Figure 6 The limiting assembly 40 also includes a resistance member 403, the resistance member 403 includes a limiting block 4031, a sliding hole 4032 is opened on the side of the installation body 401 facing the chip body 1, and the limiting block 4031 is located in the sliding hole 4032, the limiting block 4031 is plugged into the sliding hole 4032, and the limiting block 4031 is slidably connected to the inner wall of the sliding hole 4032 along the axial direction of the sliding hole 4032.

[0091] Reference Figure 5 and Figure 6 The chip body 1 contacts the limit block 4031 on one side along its length direction, and contacts the inner wall corresponding to the limit groove 101 on the other side. When the limit block 4031 extends out of the sliding hole 4032, the limit block 4031 can limit the chip body 1.

[0092] Reference Figure 5 and Figure 6 The side of the limit block 4031 away from the chip body 1 is provided with a guide surface 4033, and the guide surface 4033 is inclined, and the guide surface 4033 is inclined toward the chip body 1 along the axial direction of the slide hole 4032 away from the installation body 401. When the chip body 1 is inserted, the chip body 1 contacts the guide surface 4033, and the limit block 4031 is retracted into the slide hole 4032 under the contact of the chip body 1, which can prevent the limit block 4031 from interfering with the installation of the chip body 1.

[0093] Reference Figure 6 and Figure 7 The abutment member 403 further comprises a first spring 4034, the length direction of which is arranged along the axial direction of the slide hole 4032, and one end of the first spring 4034 is connected to the stop block 4031, and the other end is connected to the inner side wall of the slide hole 4032. The arrangement of the first spring 4034 compresses when the chip body 1 abuts against the stop block 4031 and retracts into the slide hole 4032, and then when the chip body 1 is separated from the stop block 4031, the first spring 4034 resets the stop block 4031, and at this time, the chip body 1 is located between the stop block 4031 and the inner side wall corresponding to the stop groove 101 along its own length direction, so that the chip body 1 can be automatically fixed.

[0094] Reference Figure 5 and Figure 7 The limit assembly 40 also includes a manual drive member 404, which includes a drive column 4041. A drive hole 4042 is provided on the side of the installation body 401 away from the limit groove 101. The drive hole 4042 and the slide hole 4032 are respectively located on two opposite sides of the installation body 401, and the axes of the drive hole 4042 and the slide hole 4032 are arranged parallel to each other. One end of the drive column 4041 is plugged into the drive hole 4042, and the other end of the drive column 4041 extends to the outside of the installation body 401, and the drive column 4041 is slidably connected to the inner side wall of the drive hole 4042 along its own length direction. The manual drive member 404 also includes a second spring 4043, which is located in the drive hole 4042, and the length direction of the second spring 4043 is arranged along the axial direction of the drive hole 4042. One end of the second spring 4043 is connected to the drive column 4041, and the other end is connected to the inner side wall of the drive hole 4042.

[0095] The driving column 4041 can be pushed into the driving hole 4042 by pressing the driving column 4041 along the axial direction of the driving column 4041 . After the driving column 4041 is released, the second spring 4043 can drive the driving column 4041 to return to its original position.

[0096] Reference Figure 5 and Figure 7 The limiting assembly 40 also includes a conversion member 405, which includes an intermediate rod 4051. An installation cavity 4011 is opened in the installation body 401. The intermediate rod 4051 is located in the installation cavity 4011. The middle part of the intermediate rod 4051 is rotatably connected to the installation body 401. One end of the intermediate rod 4051 is rotatably connected to the driving column 4041, and the other end is rotatably connected to the limiting block 4031. The limiting block 4031 and the driving column 4041 are both slidably connected to the intermediate rod 4051 along the length direction of the intermediate rod 4051.

[0097] When the driving column 4041 is pressed and retracted into the driving hole 4042, under the action of the middle rod 4051, the limit block 4031 is also retracted into the sliding hole 4032. At this time, the limit block 4031 releases the lock on the chip body 1, which makes it easy to remove the chip body 1 from the mounting seat 30.

[0098] Reference Figure 7, a first long hole 4052 is provided on the inner side wall of the sliding hole 4032. The length direction of the first long hole 4052 is arranged along the axial direction of the sliding hole 4032, and the first long hole 4052 communicates with the installation cavity 4011. The conversion member 405 includes a first column 4053. The first column 4053 is inserted and matched with the first long hole 4052. One end of the first column 4053 is connected to the limiting block 4031, and the other end of the first column 4053 is rotatably connected to the intermediate rod 4051. The first column 4053 is slidably connected to the intermediate rod 4051 along the length direction of the intermediate rod 4051, and the first column 4053 is slidably connected to the inner side wall of the first long hole 4052 along the length direction of the first long hole 4052. This realizes the rotational connection between the limiting block 4031 and the intermediate rod 4051.

[0099] Refer to Figure 7 , a second long hole 4054 is provided on the inner side wall of the driving hole 4042. The length direction of the second long hole 4054 is arranged along the axial direction of the driving hole 4042, and the second long hole 4054 communicates with the installation cavity 4011. The rotating member further includes a second column 4055. The second column 4055 is inserted and matched with the second long hole 4054. One end of the second column 4055 is connected to the driving column 4041, and the other end is rotatably connected to the intermediate rod 4051. The second column 4055 is slidably connected to the intermediate rod 4051 along the length direction of the intermediate rod 4051. This realizes the rotational connection between the driving column 4041 and the intermediate rod 4051.

[0100] Refer to Figure 7 , the conversion member 405 further includes an installation support rod 4012. The length direction of the installation support rod 4012 is arranged along the axial direction of the sliding hole 4032. One end of the installation support rod 4012 is connected to the inner wall of the installation cavity 4011, and the other end is rotatably connected to the middle of the intermediate rod 4051. Through the arrangement of the installation support rod 4012, the installation of the intermediate rod 4051 can be realized.

[0101] Refer to Figure 7 and Figure 8 , a pop-up assembly 50 is further provided on the inner side wall of the limiting groove 101. The pop-up assembly 50 is located on one side of the chip body 1 away from the limiting block 4031 along the length direction of the chip body 1. The pop-up assembly 50 includes a pop-up plate 501. A pop-up groove 502 is provided on the inner side wall of the limiting groove 101. The pop-up plate 501 is inserted and matched with the pop-up groove 502. The pop-up plate 501 is slidably connected to the inner side wall of the pop-up groove 502 along the depth direction of the pop-up groove 502. A plurality of pop-up springs 503 are provided between the pop-up plate 501 and the bottom of the pop-up groove 502. The axial direction of the pop-up springs 503 is arranged along the depth direction of the limiting groove 101. One end of the pop-up springs 503 is connected to the bottom of the limiting groove 101, and the other end is connected to the pop-up plate 501.

[0102] When the chip body 1 is installed in the limit groove 101, the chip body 1 abuts against the pop-up plate 501 and retracts into the pop-up groove 502. At this time, the pop-up spring 503 is compressed, and the chip body 1 is located between the pop-up plate 501 and the limit block 4031 along its own length direction. When it is necessary to remove the chip body 1, press the driving column 4041. The limit block 4031 retracts into the sliding hole 4032 under the action of the middle rod 4051. At this time, the limit of the chip body 1 by the limit block 4031 is released, the pop-up spring 503 abuts against the pop-up plate 501, and the pop-up plate 501 pops out the chip body 1, which facilitates the removal of the chip body 1.

[0103] Referring to Figure 5 , the mounting seat 30 is slidably connected to the lower clamping plate 10, and a mounting assembly 60 is arranged between the mounting seat 30 and the lower clamping plate 10. The mounting assembly 60 includes a plurality of mounting bolts 601. Mounting long holes 602 are formed on both sides in the width direction of the mounting seat 30. The length direction of the mounting long holes 602 is arranged along the length direction of the mounting seat 30. A plurality of threaded holes are formed on the lower clamping plate 10, and the plurality of threaded holes are all communicated with the corresponding mounting long holes 602. The mounting bolts 601 are arranged in one-to-one correspondence with the threaded holes. The mounting bolts 601 are inserted and threadedly connected with the corresponding threaded holes, and the nuts of the mounting bolts 601 abut against the upper side of the mounting seat 30. When the mounting bolts 601 are tightened, the fixing of the mounting seat 30 is realized; when the mounting bolts 601 are loosened, the fine adjustment of the position of the mounting seat 30 is realized.

[0104] The implementation principle of the detection device in the embodiment of the present application is as follows: during the process of nucleic acid inspection, one end of the chip body 1 in the length direction is inserted into the limit groove 101, and then the chip body 1 is pushed along the length direction of the chip body 1 until the chip body 1 is completely inserted into the limit groove 101. At this time, the limit block 4031 realizes the fixation of the position of the chip body 1, and the pop-up plate 501 can abut against the chip body 1, so that the chip body 1 will not shake or move in the limit groove 101, which can improve the installation stability and position accuracy of the chip body 1. After that, press the driving columns 4041 protruding from both sides of the mounting seat 30, the limit block 4031 releases the limit on the chip body 1, and the pop-up plate 501 pops out the chip body 1 from the limit groove 101, which realizes the disassembly of the chip body 1.

[0105] The above are all the preferred embodiments of the present application, and the protection scope of the present application is not limited by this. Therefore, all equivalent changes made according to the structure, shape and principle of the present application should be covered within the protection scope of the present application.

Claims

1. A pressure-type microfluidic chip, characterized in that: include: A chip body (1), wherein the chip body (1) has a cavity inside, and the chip body (1) is provided with a sample addition hole (2), a detection pool (3), a plurality of reaction pools (4), a plurality of reagent pools (6) and a plurality of flow slots (7); The plurality of reaction pools (4) include a sample lysis pool (41), a magnetic bead reaction pool (42) and an amplification mixing pool (44) which are connected in sequence, and the sample addition hole (2) is connected to the sample lysis pool (41), and the detection pool (3) is connected to the amplification mixing pool (44). Magnetic beads are arranged at the bottom of the magnetic bead reaction pool (42), and a drainage groove (5) is arranged on one side of the opening of the magnetic bead reaction pool (42), and the drainage groove (5) is connected to the cavity inside the chip body (1); The multiple reagent pools (6) include a washing liquid pool (61), an elution liquid pool (62), an amplification liquid pool (64) and a primer probe pool (65); the washing liquid pool (61) and the elution liquid pool (62) are respectively connected to the magnetic bead reaction pool (42); the amplification liquid pool (64) and the primer probe pool (65) are respectively connected to the amplification mixing pool (44); The plurality of flow channels (7) are used to connect the openings between adjacent reaction cells (4), and are also used to connect the opening of the reagent cell (6) with the opening of the corresponding reaction cell (4).

2. The pressure-type microfluidic chip according to claim 1, characterized in that: The bottoms of the reaction pool (4) and the reagent pool (6) are both spherical, and the concave side faces the opening direction of the reaction pool (4).

3. The pressure-type microfluidic chip according to claim 2, characterized in that: Drainage grooves (8) are provided on the inner walls of the reaction pool (4) and the reagent pool (6), and one side of the drainage groove (8) is connected to the flow groove (7) on the side of the flow direction.

4. The pressure-type microfluidic chip according to claim 3, characterized in that: A plurality of drainage grooves (8) are provided, and the plurality of drainage grooves (8) are all connected to the flow groove (7) on one side of the flow direction.

5. The pressure-type microfluidic chip according to claim 4, characterized in that: The side of the same drainage groove (8) away from the circulation groove (7) is located on the same contour plane.

6. The pressure-type microfluidic chip according to claim 1, characterized in that: The reaction pool (4) further comprises a quantitative pool (43), wherein the quantitative pool (43) is located between the magnetic bead reaction pool (42) and the amplification mixing pool (44), and one side of the quantitative pool (43) is connected to the magnetic bead reaction pool (42), and the other side is connected to the amplification mixing pool (44); The reagent pool (6) further comprises a residual liquid pool (63), wherein the residual liquid pool (63) is in communication with the quantitative pool (43).

7. The pressure-type microfluidic chip according to claim 1, characterized in that: The detection pool (3) comprises a first detection pool (31) and a second detection pool (32), and the amplification mixing pool (44), the first detection pool (31) and the second detection pool (32) are connected in sequence.

8. The pressure-type microfluidic chip according to claim 1, characterized in that: The washing liquid pool (61) comprises a first washing liquid pool (611) and a second washing liquid pool (612), and the first washing liquid pool (611) and the second washing liquid pool (612) are respectively connected to the magnetic bead reaction pool (42).

9. A detection device, characterized in that: include: The pressure-type microfluidic chip according to any one of claims 1 to 8; A lower clamping plate (10), wherein a limiting groove (101) is provided on the lower clamping plate (10), and the limiting groove (101) is used to receive the chip body (1); A magnet (102) rotatably connected to the lower clamping plate (10) to move away from or closer to the bottom of the magnetic bead reaction pool (42); An electric heating element (103) is fixedly connected to the lower clamping plate (10) and is located at the bottom of the detection pool (3); The upper clamping plate (20) is located on the upper side of the lower clamping plate (10), and a plurality of pressure hammers (201), a plurality of pressure valves (202) and a driving member (203) for driving the pressure hammers (201) and the pressure valves (202) to move are installed on the upper clamping plate (20); the pressure hammers (201) are respectively arranged in a one-to-one correspondence with the reaction pool (4), the reagent pool (6) and the detection pool (3), and the shape of the pressure hammers (201) is consistent with the inner wall of the corresponding reaction pool (4), the reagent pool (6) and the detection pool (3); the pressure valves (202) are respectively arranged in a one-to-one correspondence with the circulation groove (7) and the drainage groove (5), and the pressure valves (202) are used to close or open the corresponding circulation groove (7) or the drainage groove (5).

10. The detection device according to claim 9, characterized in that: The lower clamping plate (10) is also equipped with an ultrasonic device (104), and the ultrasonic device (104) is located at the bottom of the sample lysis pool (41).

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