A yeast-derived bio-organic fertilizer suitable for aquaculture, a preparation method and application thereof

Abstract

The application belongs to the technical field of fertilizers, and particularly relates to a yeast-source biological organic fertilizer suitable for aquaculture and a preparation method and application thereof. The yeast-source biological organic fertilizer suitable for aquaculture comprises the following components in parts by weight: 40-80 parts of yeast metabolites, 15-30 parts of ammonium chloride, 10-20 parts of monoammonium phosphate and 3-8 parts of a compound microbial agent. The yeast-source biological organic fertilizer suitable for aquaculture has reasonable proportioning, a high number of beneficial bacteria, a good symbiotic environment of multiple bacteria and strong biological activity. The organic active nutrients, effective microbial groups and inorganic nutrients are complete and scientifically proportioned, and the fertilizer has good effects of fertilization, water culture and algae cultivation, can improve the aquaculture water environment, supplement feed organisms, promote the growth of aquaculture objects, improve product benefits and is more conducive to environmental protection.

Description

Technical Field

[0001] This invention belongs to the field of fertilizer technology, specifically relating to a yeast-derived bio-organic fertilizer suitable for aquaculture, its preparation method, and its application. Background Technology

[0002] As the saying goes, "To raise fish, first raise water," and in the aquaculture industry, water management is crucial. Indeed, water quality directly impacts the success of aquaculture. Good water quality not only provides ample natural food for fish and shrimp but also improves the ecological environment of the reservoir, promoting their healthy growth. Currently, there are many types of fertilizers available for aquaculture, mainly divided into organic and inorganic fertilizers. Inorganic fertilizers are relatively quick to fertilize water, but excessive use can lead to nutrient accumulation, causing eutrophication, which harms water quality and fish / shrimp, and its effects are short-lived. Organic fertilizers, on the other hand, have a relatively simple nutritional profile. Therefore, traditional fertilizers can no longer meet market demands.

[0003] To alleviate the above problems, a large number of bio-fertilizers containing active microorganisms have emerged in recent years. These are green, environmentally friendly, and eco-friendly fertilizers that not only provide nutrients but also improve water quality, inhibit the growth of harmful bacteria, and effectively promote the growth of fish and shrimp. However, single-microbial agents suffer from limitations such as limited functionality and poor adaptability. Therefore, there is an urgent need to develop a fertilizer containing microbial agents that can effectively improve water quality and promote the growth of fish, shrimp, and shellfish, thereby meeting the requirements of high-yield, high-efficiency, and environmentally friendly modern fisheries. Summary of the Invention

[0004] The technical problem solved by this invention is that, in the prior art, most bio-fertilizers suitable for aquaculture are products containing only a single effective bacteria, and the activity of the bacteria is relatively weak, resulting in low fertilizer efficiency and failing to meet market demand.

[0005] To address the aforementioned technical problems, this invention provides a yeast-derived bio-organic fertilizer suitable for aquaculture, its preparation method, and its application.

[0006] Specifically, the present invention provides the following technical solution:

[0007] In a first aspect, the present invention provides a yeast-derived bio-organic fertilizer suitable for aquaculture, comprising, by weight: 40-80 parts of yeast metabolites, 15-30 parts of ammonium chloride, 10-20 parts of monoammonium phosphate, and 3-8 parts of compound microbial agent.

[0008] Preferably, by weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40-70 parts of yeast metabolites; and / or, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 15-25 parts of ammonium chloride; and / or, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 10-15 parts of monoammonium phosphate; and / or, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 3-6 parts of compound microbial agent.

[0009] Preferably, the compound microbial agent includes one or more substances selected from the group consisting of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterosporus, and Bacillus coagulans.

[0010] Preferably, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens is 3-7:2-6:0.5-3; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus is 3-7:2-6:0.5-3; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans is 3-7:2-6:0.5-3.

[0011] Preferably, the compound microbial agent has a bacterial count of 90-110 billion CFU / g; and / or, the yeast-derived bio-organic fertilizer suitable for aquaculture has a total content of N, P2O5, and K2O of more than 10% on a dry basis; and / or, a pH value of 4.5-8.5; and / or, a density of 1.2-1.3 g / cm³. 3 .

[0012] Secondly, the present invention provides a method for preparing yeast-derived bio-organic fertilizer suitable for aquaculture, comprising the following steps:

[0013] Yeast metabolites, ammonium chloride, monoammonium phosphate, and compound bacterial agents are mixed to obtain yeast-derived bio-organic fertilizer suitable for aquaculture.

[0014] Preferably, the yeast metabolite is a metabolite extracted from yeast through fermentation using molasses or hydrolyzed sugar as a culture medium; and / or, by weight, the yeast metabolite includes 1%–5% total nitrogen, 11%–16% potassium, 2%–8% sodium, 34%–40% organic matter, 32%–38% fulvic acid, and / or 8%–14% amino acids.

[0015] Thirdly, the present invention provides a yeast-derived bio-organic fertilizer suitable for aquaculture, which is prepared by the above-mentioned method for preparing yeast-derived bio-organic fertilizer suitable for aquaculture.

[0016] Fourthly, this invention provides an application of yeast-derived bio-organic fertilizer suitable for aquaculture in the preparation of fertilized water.

[0017] Fifthly, the present invention provides the application of a yeast-derived bio-organic fertilizer suitable for aquaculture in reducing ammonia nitrogen content in water, increasing chlorophyll content in water, and increasing the types and content of algae in water, or in one or more of these functions.

[0018] In a sixth aspect, the present invention provides a fertilizer agent comprising the above-mentioned yeast-derived bio-organic fertilizer suitable for aquaculture.

[0019] In a seventh aspect, the present invention provides a composition having one or more of the following functions: reducing ammonia nitrogen content in water, increasing chlorophyll content in water, and increasing the types and content of algae, wherein the composition comprises the above-mentioned yeast-derived bio-organic fertilizer suitable for aquaculture.

[0020] Beneficial effects of the present invention

[0021] (1) The yeast-derived bio-organic fertilizer in this invention is suitable for aquaculture and has a reasonable ratio. The number of beneficial bacteria it contains is much higher than that of ordinary bio-organic fertilizer. It has a better multi-bacterial symbiotic environment and stronger biological activity. Moreover, it is easy to obtain materials, inexpensive, has good fertilization effect, high fertilizer efficiency, can greatly improve yield and income, and is more conducive to environmental protection.

[0022] (2) The yeast-derived bio-organic fertilizer for aquaculture in this invention contains organic matter, effective microbial community and chemical nutrients.

[0023] (3) The yeast-derived bio-organic fertilizer for aquaculture in this invention can significantly reduce the content of ammonia nitrogen in water, has a good effect of fertilizing water and cultivating algae, can improve the aquatic environment, supplement feed organisms, promote the growth of aquaculture organisms, and increase product yield.

[0024] (4) The yeast-derived bio-organic fertilizer of the present invention, which is suitable for aquaculture, has been tested by 27 fish, shrimp and shellfish farmers in the Bohai Rim region, including 5 large-scale marine aquaculture farms. All of them reported that the fertilizer effect was good and that it was beneficial to reduce the cost of aquaculture. Detailed Implementation

[0025] As described above, the purpose of this invention is to provide a yeast-derived bio-organic fertilizer suitable for aquaculture, its preparation method, and its application.

[0026] This invention provides the following technical solution:

[0027] Technical Solution 1. A yeast-derived bio-organic fertilizer suitable for aquaculture, comprising, by weight: 40-80 parts yeast metabolites, 15-30 parts ammonium chloride, 10-20 parts monoammonium phosphate, and 3-8 parts compound microbial agent.

[0028] Technical Solution 2. The yeast-derived bio-organic fertilizer suitable for aquaculture according to Technical Solution 1, wherein, by weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40-70 parts of yeast metabolites; preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40-50 parts of yeast metabolites; more preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40-42 parts of yeast metabolites.

[0029] And / or, the yeast-derived bio-organic fertilizer suitable for aquaculture includes 15-25 parts of ammonium chloride; preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture includes 15-17 parts of ammonium chloride;

[0030] And / or, the yeast-derived bio-organic fertilizer suitable for aquaculture includes 10-15 parts of monoammonium phosphate; preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture includes 10-12 parts of monoammonium phosphate.

[0031] And / or, the yeast-derived bio-organic fertilizer suitable for aquaculture includes 3 to 6 parts of compound microbial agent; preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture includes 3 to 5 parts of compound microbial agent.

[0032] Technical Solution 3. The yeast-derived bio-organic fertilizer suitable for aquaculture according to Technical Solution 1 or 2, wherein the compound microbial agent includes one or more of the following substances selected from the group consisting of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterosporus, and Bacillus coagulans.

[0033] Preferably, the compound microbial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens; or, the compound microbial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus; or, the compound microbial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans.

[0034] More preferably, the compound microbial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens; or, the compound microbial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans.

[0035] More preferably, the compound microbial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens.

[0036] Technical Solution 4. The yeast-derived bio-organic fertilizer suitable for aquaculture according to Technical Solution 3, wherein the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens is 3-7:2-6:0.5-3; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus is 3-7:2-6:0.5-3; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans is 3-7:2-6:0.5-3;

[0037] Preferably, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens is 4.5–6:3.5–5:1–2; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus is 4.5–6:3.5–5:1–2; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans is 4.5–6:3.5–5:1–2.

[0038] More preferably, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens is 4.8–5.2:3.5–4:1–1.5; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus is 4.8–5.2:3.5–4:1–1.5; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans is 4.8–5.2:3.5–4:1–1.5.

[0039] Technical Solution 5. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-4, wherein the number of bacteria in the compound microbial agent is 90-110 billion CFU / g;

[0040] And / or, the yeast-derived bio-organic fertilizer suitable for aquaculture, on a dry basis, has a total content of N, P2O5, and K2O of more than 10%; and / or, a pH value of 4.5–8.5; and / or, a density of 1.2–1.3 g / cm³. 3 ;

[0041] Preferably, the total content of N, P2O5 and K2O is 10% to 30%; more preferably, the total content of N, P2O5 and K2O is 20% to 30%.

[0042] Technical Solution 6. A method for preparing yeast-derived bio-organic fertilizer suitable for aquaculture as described in any of Technical Solutions 1-5, comprising the following steps:

[0043] Yeast metabolites, ammonium chloride, monoammonium phosphate, and compound microbial agents are mixed to obtain yeast-derived bio-organic fertilizer suitable for aquaculture.

[0044] Technical Solution 7. A method for preparing yeast-derived bio-organic fertilizer suitable for aquaculture according to Technical Solution 6, wherein the yeast metabolites are the metabolic products extracted from yeast through fermentation using molasses or hydrolyzed sugar as a culture medium; preferably, molasses is used as a culture medium, and brewer's yeast is inoculated onto the culture medium for fermentation. After fermentation, the mixture is separated, and the supernatant is concentrated to obtain a yeast fermentation concentrate, which is then dried to obtain the yeast metabolites; more preferably, the molasses is beet molasses or sugarcane molasses; and / or, more preferably, the pH of the molasses culture medium is 4.5–6.0; and / or, more preferably, the fermentation conditions are: a temperature of 25℃–33℃, and / or a time of 12h–18h; and / or, more preferably, the dry matter mass percentage in the yeast fermentation concentrate is 45%–55%; and / or, more preferably, the drying is spray drying; even more preferably, the inlet air temperature is 230–260℃ and / or the outlet air temperature is 140–160℃;

[0045] Technical Solution 8. A method for preparing yeast-derived bio-organic fertilizer suitable for aquaculture according to Technical Solution 6 or 7, wherein, by weight of yeast metabolites, the yeast metabolites include a total nitrogen content of 1% to 5%, a potassium content of 11% to 16%, a sodium content of 2% to 8%, an organic matter content of 34% to 40%, a fulvic acid content of 32% to 38%, and / or an amino acid content of 8% to 14%.

[0046] Technical Solution 9. A yeast-derived bio-organic fertilizer suitable for aquaculture, characterized in that the yeast-derived bio-organic fertilizer suitable for aquaculture is prepared by any one of the preparation methods of yeast-derived bio-organic fertilizer suitable for aquaculture in technical solutions 6-8.

[0047] Technical Solution 10. The application of the yeast-derived bio-organic fertilizer for aquaculture as described in any one of technical solutions 1-5 or the yeast-derived bio-organic fertilizer for aquaculture as described in technical solution 9 in the preparation of fertilized water;

[0048] And / or, the application of the yeast-derived bio-organic fertilizer suitable for aquaculture in reducing ammonia nitrogen content in water;

[0049] And / or, the application of the yeast-derived bio-organic fertilizer suitable for aquaculture in increasing chlorophyll content in water;

[0050] And / or, the application of the yeast-derived bio-organic fertilizer suitable for aquaculture in increasing the types and content of algae.

[0051] Technical Solution 11. A fertilizer agent, wherein the fertilizer agent comprises the yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of Technical Solutions 1-5 or the yeast-derived bio-organic fertilizer suitable for aquaculture as described in Technical Solution 9.

[0052] Technical Solution 12. A composition having one or more of the following functions: reducing ammonia nitrogen content in water, increasing chlorophyll content in water, and increasing the types and content of algae, wherein the composition comprises the yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of Technical Solutions 1-5 or the yeast-derived bio-organic fertilizer suitable for aquaculture as described in Technical Solution 9.

[0053] Technical Solution 13. The yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5 or the yeast-derived bio-organic fertilizer suitable for aquaculture according to Technical Solution 9, wherein the yeast metabolites are prepared by using molasses as a culture medium, inoculating Saccharomyces cerevisiae onto the culture medium for fermentation, separating the yeast metabolites after fermentation, concentrating the supernatant to obtain yeast fermentation concentrate, and drying the concentrate to obtain yeast metabolites.

[0054] Technical Solution 14. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5, or a yeast-derived bio-organic fertilizer suitable for aquaculture according to Technical Solutions 9 or 13, wherein the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40-80 parts of yeast metabolites. For example, in some embodiments, the yeast-derived bio-organic fertilizer comprises 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, or 80 parts of yeast metabolites, or contains yeast metabolites within a numerical range defined by any two of the above specific values ​​as endpoints.

[0055] Technical Solution 15. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5, or a yeast-derived bio-organic fertilizer suitable for aquaculture according to Technical Solutions 9, 13, or 14, wherein the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 15-30 parts of ammonium chloride. For example, in some embodiments, the yeast-derived bio-organic fertilizer comprises 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 parts of ammonium chloride, or contains ammonium chloride within a numerical range defined by any two of the above specific values ​​as endpoints. The present invention selects ammonium chloride as the nitrogen source because, compared with other nitrogen sources, ammonium chloride has a high nitrogen content, good stability, is easily absorbed, and is relatively inexpensive; moreover, when ammonium chloride is used as a nitrogen source and is compounded with yeast metabolites, the compounded product is less prone to moisture absorption and caking, resulting in better product quality stability.

[0056] Technical Solution 16. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5 or any one of Technical Solutions 9 or 13-15, wherein the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 10-20 parts of monoammonium phosphate. For example, in some embodiments, the yeast-derived bio-organic fertilizer comprises 10, 10.5, 11 / 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 15.5, 16, 16.5, 17, 17.5, 18, 18.5, 19, 19.5 or 20 parts of monoammonium phosphate, or contains monoammonium phosphate within a numerical range formed by any two of the above specific values ​​as endpoints. The monoammonium phosphate selected in this invention is chosen because, compared with other phosphorus sources, agricultural-grade monoammonium phosphate has a reasonable nitrogen and phosphorus content, good stability, good water solubility, is easy to absorb, and is relatively inexpensive. Moreover, when monoammonium phosphate is used as a phosphorus source and is compounded with yeast metabolites, the compounded product is not prone to moisture absorption and caking, and the product quality stability is good.

[0057] Technical Solution 17. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5, or a yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 9 or 13-16, wherein the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 3 to 8 parts of compound microbial agent. For example, in some embodiments, the yeast-derived bio-organic fertilizer comprises 3, 3.2, 3.4, 3.6, 3.8, 4, 4.2, 4.4, 4.6, 4.8, 5, 5.2, 5.4, 5.6, 5.8, 6, 6.2, 6.4, 6.6, 6.8, 7, 7.2, 7.4, 7.6, 7.8, or 8 parts of compound microbial agent, or contains compound microbial agent within the numerical range formed by any two of the above specific values ​​as endpoints.

[0058] Technical Solution 18. The yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5 or any one of Technical Solutions 9 or 13-17, wherein, by weight, the compound microbial agent contains 3 to 7 parts of Bacillus subtilis. For example, in some embodiments, the compound microbial agent includes 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9 or 7 parts of Bacillus subtilis, or contains Bacillus subtilis within the numerical range formed by any two of the above specific values ​​as endpoints.

[0059] Technical Solution 19. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5 or any one of Technical Solutions 9 or 13-18, wherein, by weight, the compound microbial agent contains 2-6 parts of Bacillus licheniformis. For example, in some embodiments, the compound microbial agent includes 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, or 6 parts of Bacillus licheniformis, or contains Bacillus licheniformis within a numerical range defined by any two of the above specific values ​​as endpoints.

[0060] Technical Solution 20. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5 or any one of Technical Solutions 9 or 13-19, wherein, by weight, the compound microbial agent contains 0.5 to 3 parts of Bacillus amyloliquefaciens. For example, in some embodiments, the compound microbial agent includes 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, or 3 parts of Bacillus amyloliquefaciens, or contains Bacillus amyloliquefaciens within the numerical range formed by any two of the above specific values ​​as endpoints.

[0061] Technical Solution 21. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5 or any one of Technical Solutions 9 or 13-20, wherein, by weight, the compound microbial agent contains 0.5 to 3 parts of Bacillus laterosporus. For example, in some embodiments, the compound microbial agent includes 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, or 3 parts of Bacillus laterosporus, or contains Bacillus laterosporus within a numerical range defined by any two of the above specific values ​​as endpoints.

[0062] Technical Solution 22. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5 or any one of Technical Solutions 9 or 13-21, wherein, by weight, the compound microbial agent contains 0.5 to 3 parts of Bacillus coagulans. For example, in some embodiments, the compound microbial agent includes 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, or 3 parts of Bacillus coagulans, or contains Bacillus coagulans within the numerical range formed by any two of the above specific values ​​as endpoints.

[0063] Technical Solution 23. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5 or any one of Technical Solutions 9 or 13-22, wherein the preparation method of the compound microbial agent includes the following steps: first, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens are mixed to obtain a microbial mixture, and then zeolite powder and calcium carbonate are added and mixed evenly to obtain the compound microbial agent.

[0064] Technical Solution 24. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5, or a yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 9 or 13-23, wherein, based on the mass parts of the compound microbial agent, the compound microbial agent comprises 45-55 parts of a microbial inoculum mixture, 20-30 parts of zeolite powder, and 20-30 parts of calcium carbonate. More preferably, the compound microbial agent comprises 48-52 parts of a microbial inoculum mixture, 23-27 parts of zeolite powder, and 23-27 parts of calcium carbonate. Even more preferably, the compound microbial agent comprises 49-51 parts of a microbial inoculum mixture, 24-26 parts of zeolite powder, and 24-26 parts of calcium carbonate.

[0065] Technical Solution 25. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5, or a yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 9 or 13-24, wherein the zeolite powder refers to powder made by grinding zeolite. More preferably, any commercially available or self-made zeolite can be used to prepare the zeolite powder of the present invention. Even more preferably, the zeolite used in the zeolite powder includes one or more substances selected from the group consisting of sodium zeolite, analcime, clinoptilolite, chalcogenide, flaky zeolite, mordenite, zeolite, zeolite, calcium cruciformite, and zeolite.

[0066] Technical Solution 26. The application of the yeast-derived bio-organic fertilizer for aquaculture as described in Technical Solution 10 in reducing the ammonia nitrogen content in water, wherein, on the first day after adding the yeast-derived bio-organic fertilizer, the ammonia nitrogen removal rate is 12% to 17%; preferably, on the first day after adding the yeast-derived bio-organic fertilizer, the ammonia nitrogen removal rate is 13% to 15%.

[0067] And / or, on the second day after adding yeast-derived bio-organic fertilizer, the removal rate of ammonia nitrogen is 16% to 50%; preferably, on the second day after adding yeast-derived bio-organic fertilizer, the removal rate of ammonia nitrogen is 16% to 20%.

[0068] And / or, on the 3rd day after adding yeast-derived bio-organic fertilizer, the removal rate of ammonia nitrogen is 25% to 50%; preferably, on the 3rd day after adding yeast-derived bio-organic fertilizer, the removal rate of ammonia nitrogen is 30% to 32%.

[0069] And / or, on the 4th day after adding yeast-derived bio-organic fertilizer, the removal rate of ammonia nitrogen is 25% to 80%; preferably, on the 4th day after adding yeast-derived bio-organic fertilizer, the removal rate of ammonia nitrogen is 55% to 80%; more preferably, on the 4th day after adding yeast-derived bio-organic fertilizer, the removal rate of ammonia nitrogen is 70% to 80%.

[0070] Technical Solution 27. The application of the yeast-derived bio-organic fertilizer for aquaculture as described in Technical Solution 10 in increasing the chlorophyll content in water, wherein on the first day after adding the yeast-derived bio-organic fertilizer, the chlorophyll a content is 56-69 mg / m³. 3 Preferably, on the first day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content is 65–68 mg / m³. 3 ;

[0071] And / or, on the second day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content was 73–104 mg / m³. 3 Preferably, on the second day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content is 73–100 mg / m³. 3 ;

[0072] And / or, on the 3rd day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content was 103–145 mg / m³. 3 Preferably, on the 3rd day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content is 129–145 mg / m³. 3 More preferably, on the 3rd day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content is 138–145 mg / m³. 3 ;

[0073] And / or, on the 4th day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content was 125–157 mg / m³. 3 Preferably, on the 4th day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content is 138–157 mg / m³. 3 More preferably, on the 4th day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content is 147–157 mg / m³. 3 ;

[0074] And / or, on the 5th day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content was 105–143 mg / m³. 3 Preferably, on the 5th day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content is 125–143 mg / m³. 3 More preferably, on the 5th day after adding yeast-derived bio-organic fertilizer, the chlorophyll a content is 142–143 mg / m³. 3 .

[0075] Technical Solution 28. The application of yeast-derived bio-organic fertilizer suitable for aquaculture according to Technical Solution 10 in improving the types and content of algae, wherein the algae include one or more of the following groups: *Platycodon grandiflorus* sp2, *Cynodon spheroides*, *Cynodon spheroides*, *Scenedesmus*, *Hylocereus*, *Cryptocybe*, and *Cyclotella*.

[0076] Technical Solution 29. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5, or a yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 9 or 13-25. The present invention does not limit the source of the yeast metabolites; any commercially available or self-made yeast metabolites can be used in the present invention. Specifically, the inventors have found that as long as the commercially available or self-made yeast metabolites have a total nitrogen content ≥1%, potassium content ≥11%, sodium content ≥2%, organic matter content ≥25%, fulvic acid content ≥25%, and / or amino acid content ≥8%, they can be used in the present invention. Preferably, the yeast metabolites include a total nitrogen content of 1%–5%, a potassium content of 11%–16%, a sodium content of 2%–8%, an organic matter content of 34%–40%, a fulvic acid content of 32%–38%, and / or an amino acid content of 8%–14%. More preferably… The yeast metabolites comprise a total nitrogen content of 2.6%–3.6%, a potassium content of 13.5%–14.5%, a sodium content of 5%–6%, an organic matter content of 36.7%–37.7%, a fulvic acid content of 35%–36%, and / or an amino acid content of 10.7%–11.7%; most preferably, the yeast metabolites comprise a total nitrogen content of 3.19%, a potassium content of 13.92%, a sodium content of 5.78%, an organic matter content of 37.2%, a fulvic acid content of 35.36%, and / or an amino acid content of 11.2%.

[0077] Technical Solution 30. A yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of Technical Solutions 1-5, or a yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of Technical Solutions 9, 29, or 13-25. The present invention does not limit the source of Bacillus subtilis; any commercially purchased or self-made Bacillus subtilis can be used in the present invention. Preferably, the inventors have found that as long as the viable count of commercially purchased or self-made Bacillus subtilis is ≥15 billion CFU / g, it can be used in the present invention. The viable count is preferably 190-250 billion CFU / g, more preferably 190-210 billion CFU / g, further preferably 195-205 billion CFU / g, and even more preferably 199-201 billion CFU / g.

[0078] Technical Solution 31. A yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 1-5, or a yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of Technical Solutions 9, 29, 30, or 13-25, wherein the present invention does not limit the source of Bacillus licheniformis, and any commercially purchased or self-made Bacillus licheniformis can be used in the present invention; preferably, the inventors have found through research that as long as the viable count of commercially purchased or self-made Bacillus licheniformis is ≥15 billion CFU / g, it can be used in the present invention, and the viable count is preferably 190-250 billion CFU / g, more preferably 190-210 billion CFU / g, further preferably 195-205 billion CFU / g, and more preferably 199-201 billion CFU / g.

[0079] Technical Solution 32. A yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of Technical Solutions 1-5, or any one of Technical Solutions 9, 29-31, or 13-25, wherein the present invention does not limit the source of Bacillus laterosporus, and any commercially purchased or self-made Bacillus laterosporus can be used in the present invention; preferably, the inventors have found through research that as long as the viable count of commercially purchased or self-made Bacillus laterosporus is ≥15 billion CFU / g, it can be used in the present invention, and the viable count is preferably 190-250 billion CFU / g, more preferably 190-210 billion CFU / g, further preferably 195-205 billion CFU / g, and more preferably 199-201 billion CFU / g.

[0080] Technical Solution 33. A yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of Technical Solutions 1-5, or any one of Technical Solutions 9, 29-32, or 13-25, wherein the present invention does not limit the source of Bacillus coagulans and any commercially purchased or self-made Bacillus coagulans can be used in the present invention; preferably, the inventors have found through research that as long as the viable count of commercially purchased or self-made Bacillus coagulans is ≥15 billion CFU / g, it can be used in the present invention, and the viable count is preferably 190-250 billion CFU / g, more preferably 190-210 billion CFU / g, further preferably 195-205 billion CFU / g, and more preferably 199-201 billion CFU / g.

[0081] Technical Solution 34. A yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of Technical Solutions 1-5, or any one of Technical Solutions 9, 29-33, or 13-25, wherein the present invention does not limit the source of Bacillus coagulans and any commercially purchased or self-made Bacillus amyloliquefaciens can be used in the present invention; preferably, the inventors have found through research that as long as the viable count of commercially purchased or self-made Bacillus amyloliquefaciens is ≥15 billion CFU / g, it can be used in the present invention, and the viable count is preferably 190-250 billion CFU / g, more preferably 190-210 billion CFU / g, further preferably 195-205 billion CFU / g, and more preferably 199-201 billion CFU / g.

[0082] Unless otherwise stated, all reagents / instruments used in the embodiments and comparative examples of this invention are conventional commercially available products. Information on the experimental materials used in this invention is provided in Table 1 below.

[0083] Table 1 Experimental Materials and Suppliers

[0084]

[0085] In this invention, the method for detecting total nitrogen content refers to section 3.1, distillation titration method (arbitration method), of the People's Republic of China Agricultural Industry Standard NY / T1977-2010, "Determination of Total Nitrogen, Phosphorus, and Potassium Content in Water-Soluble Fertilizers". The method for detecting potassium content refers to section 5, "Determination of Potassium Content," of the People's Republic of China Agricultural Industry Standard NY / T1977-2010, "Determination of Total Nitrogen, Phosphorus, and Potassium Content in Water-Soluble Fertilizers," specifically the potassium tetraphenylborate gravimetric method. The method for detecting phosphorus content refers to section 4, "Determination of Phosphorus Content," of the People's Republic of China Agricultural Industry Standard NY / T1977-2010, "Determination of Total Nitrogen, Phosphorus, and Potassium Content in Water-Soluble Fertilizers," specifically the quinoline phosphomolybdate gravimetric method. The method for detecting sodium content refers to section 3.1, "Flame Photometry," of the People's Republic of China Agricultural Industry Standard NY / T1972-2010, "Determination of Sodium, Selenium, and Silicon Content in Water-Soluble Fertilizers". The method for detecting organic matter content refers to the People's Republic of China Agricultural Industry Standard NY / T1976-2010, "Determination of Organic Matter Content in Water-Soluble Fertilizers". The method for determining fulvic acid content refers to section 4.6, "Determination of Humic Acid (Total Humic Acid or Soluble Humic Acid) and Fulvic Acid Content (Volume Method)," in the People's Republic of China Chemical Industry Standard HG / T 3276-2019, "Analytical Methods for Ammonium Humate Fertilizers." The method for determining amino acid content refers to section 3, "Conventional Acid Hydrolysis Method," in the People's Republic of China National Standard GB / T 18246-2019, "Determination of Amino Acids in Feed."

[0086] To better understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below with reference to specific embodiments.

[0087] Example 1

[0088] (1) Formula for yeast-derived bio-organic fertilizer suitable for aquaculture

[0089] A yeast-derived bio-organic fertilizer suitable for aquaculture comprises, by weight, 60 parts yeast metabolites, 22 parts ammonium chloride, 14 parts monoammonium phosphate, and 4 parts compound microbial agent. The compound microbial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens in a mass ratio of 5:4:1. The viable count in the compound microbial agent is 10 billion CFU / g.

[0090] The preparation method of the compound microbial agent includes the following steps: first, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens are mixed in a mass ratio of 5:4:1 to obtain a microbial mixture, and then zeolite powder and calcium carbonate are added and mixed evenly to obtain the compound microbial agent; wherein, based on the total mass of the compound microbial agent, the compound microbial agent includes 50% microbial mixture, 25% zeolite powder and 25% calcium carbonate.

[0091] The yeast metabolites were purchased from Angel Yeast (Binzhou) Co., Ltd. The yeast metabolites comprise, by weight, 3.19% total nitrogen, 13.92% potassium, 5.78% sodium, 37.2% organic matter, 35.36% fulvic acid, and 11.2% amino acids.

[0092] (2) Preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture

[0093] A method for preparing yeast-derived bio-organic fertilizer suitable for aquaculture includes the following steps:

[0094] Yeast metabolites are fed into the feeding pipe, while ammonium chloride, monoammonium phosphate, and compound microbial agents, already added to the feed hopper of the auxiliary feed addition system, are simultaneously fed into the same pipe according to the formula ratio for yeast-derived bio-organic fertilizer suitable for aquaculture. The flow rates of the yeast metabolites, ammonium chloride, monoammonium phosphate, and compound microbial agents are regulated by their respective flow control valves to ensure thorough and uniform mixing in the sample collection pipe. The uniformly mixed sample is collected in the packaging hopper and released through the lower port for further packaging.

[0095] The product specifications of the yeast-derived bio-organic fertilizer prepared for aquaculture are as follows: total nutrients (sum of N, P2O5, and K2O) (on a dry basis) 26%; moisture 0.32%; pH 6.6; density 1.25 g / cm³. 3 .

[0096] Example 2

[0097] (1) A formula for a yeast-derived bio-organic fertilizer suitable for aquaculture

[0098] A yeast-derived bio-organic fertilizer suitable for aquaculture, by weight, comprises 40 parts yeast metabolites, 15 parts ammonium chloride, 10 parts monoammonium phosphate, and 3 parts compound microbial agent. The compound microbial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens in a mass ratio of 5:4:1. The viable count in the compound microbial agent is 10 billion CFU / g.

[0099] The preparation method of the compound microbial agent is the same as that in Example 1. The yeast metabolites are the same as those in Example 1.

[0100] (2) The preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture is the same as the preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture in Example 1.

[0101] The product specifications of the yeast-derived bio-organic fertilizer prepared for aquaculture are as follows: total nutrients (sum of N, P2O5, and K2O) (based on the dry weight of the organic fertilizer) 25%; moisture 0.43%; pH 5.9; density 1.28 g / cm³. 3 .

[0102] Example 3

[0103] (1) A formula for a yeast-derived bio-organic fertilizer suitable for aquaculture

[0104] A yeast-derived bio-organic fertilizer suitable for aquaculture, by weight, comprises 80 parts yeast metabolites, 30 parts ammonium chloride, 20 parts monoammonium phosphate, and 8 parts compound microbial agent. The compound microbial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens in a mass ratio of 5:4:1. The viable count in the compound microbial agent is 10 billion CFU / g.

[0105] The preparation method of the compound microbial agent is the same as that in Example 1. The yeast metabolites are the same as those in Example 1.

[0106] (2) The preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture is the same as the preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture in Example 1.

[0107] The product specifications of the yeast-derived bio-organic fertilizer prepared for aquaculture are as follows: total nutrients (sum of N, P2O5, and K2O) (based on the dry weight of the organic fertilizer) 27%; moisture 0.49%; pH 6.9; density 1.26 g / cm³. 3 .

[0108] Example 4

[0109] (1) A formula for a yeast-derived bio-organic fertilizer suitable for aquaculture

[0110] A yeast-derived bio-organic fertilizer suitable for aquaculture, by weight, comprises 60 parts yeast metabolites, 25 parts ammonium chloride, 10 parts monoammonium phosphate, and 5 parts compound microbial agent. The compound microbial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens in a mass ratio of 6:5:2. The viable count in the compound microbial agent is 10 billion CFU / g.

[0111] The preparation method of the compound microbial agent includes the following steps: first, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens are mixed in a mass ratio of 6:5:2 to obtain a microbial mixture, and then zeolite powder and calcium carbonate are added and mixed evenly to obtain the compound microbial agent; wherein, based on the total mass of the compound microbial agent, the compound microbial agent includes 50% microbial mixture, 25% zeolite powder and 25% calcium carbonate.

[0112] The yeast metabolites mentioned herein are the same as those in Example 1.

[0113] (2) The preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture is the same as the preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture in Example 1.

[0114] The product specifications of the yeast-derived bio-organic fertilizer prepared for aquaculture are as follows: total nutrients (sum of N, P2O5, and K2O) (based on the dry weight of the organic fertilizer) 23%; moisture 0.35%; pH 7; density 1.24 g / cm³. 3 .

[0115] Example 5

[0116] (1) A formula for a yeast-derived bio-organic fertilizer suitable for aquaculture

[0117] A yeast-derived bio-organic fertilizer suitable for aquaculture comprises, by weight, 42 parts yeast metabolites, 30 parts ammonium chloride, 20 parts monoammonium phosphate, and 8 parts compound microbial agent. The compound microbial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens in a mass ratio of 4:3:1. The viable count in the compound microbial agent is 10 billion CFU / g.

[0118] The preparation method of the compound microbial agent includes the following steps: first, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens are mixed in a mass ratio of 4:3:1 to obtain a microbial mixture, and then zeolite powder and calcium carbonate are added and mixed evenly to obtain the compound microbial agent; wherein, based on the total mass of the compound microbial agent, the compound microbial agent includes 50% microbial mixture, 25% zeolite powder and 25% calcium carbonate.

[0119] The yeast metabolites mentioned herein are the same as those in Example 1.

[0120] (2) The preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture is the same as the preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture in Example 1.

[0121] The product specifications of the yeast-derived bio-organic fertilizer prepared for aquaculture are as follows: total nutrients (sum of N, P2O5, and K2O) (based on the dry weight of the organic fertilizer) 29%; moisture 0.37%; pH 6.2; density 1.26 g / cm³. 3 .

[0122] Example 6

[0123] (1) A formula for a yeast-derived bio-organic fertilizer suitable for aquaculture

[0124] A yeast-derived bio-organic fertilizer suitable for aquaculture comprises, by weight, 60 parts yeast metabolites, 22 parts ammonium chloride, 14 parts monoammonium phosphate, and 4 parts compound microbial agent. The compound microbial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus in a mass ratio of 5:3.5:1.5. The viable count in the compound microbial agent is 10 billion CFU / g.

[0125] The preparation method of the compound microbial agent includes the following steps: first, Bacillus subtilis, Bacillus licheniformis and Bacillus laterosporus are mixed in a mass ratio of 5:3.5:1.5 to obtain a microbial mixture, and then zeolite powder and calcium carbonate are added and mixed evenly to obtain the compound microbial agent; wherein, based on the total mass of the compound microbial agent, the compound microbial agent includes 50% microbial mixture, 25% zeolite powder and 25% calcium carbonate.

[0126] The yeast metabolites mentioned herein are the same as those in Example 1.

[0127] (2) The preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture is the same as the preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture in Example 1.

[0128] The product specifications of the yeast-derived bio-organic fertilizer prepared for aquaculture are as follows: total nutrients (sum of N, P2O5, and K2O) (based on the dry weight of the organic fertilizer) 26%; moisture 0.29%; pH 6.5; density 1.25 g / cm³. 3 .

[0129] Example 7

[0130] (1) A formula for a yeast-derived bio-organic fertilizer suitable for aquaculture

[0131] A yeast-derived bio-organic fertilizer suitable for aquaculture comprises, by weight, 40 parts yeast metabolites, 15 parts ammonium chloride, 10 parts monoammonium phosphate, and 3 parts compound microbial agent. The compound microbial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans in a mass ratio of 5:3.5:1.5. The viable count in the compound microbial agent is 10 billion CFU / g.

[0132] The preparation method of the compound microbial agent includes the following steps: first, Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans in the compound microbial agent are mixed in a mass ratio of 5:3.5:1.5 to obtain a microbial mixture; then, zeolite powder and calcium carbonate are added and mixed evenly to obtain the compound microbial agent; wherein, based on the total mass of the compound microbial agent, the compound microbial agent includes 50% microbial mixture, 25% zeolite powder and 25% calcium carbonate.

[0133] The yeast metabolites mentioned herein are the same as those in Example 1.

[0134] (2) The preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture is the same as the preparation method of yeast-derived bio-organic fertilizer suitable for aquaculture in Example 1.

[0135] The product specifications of the yeast-derived bio-organic fertilizer prepared for aquaculture are as follows: total nutrients (sum of N, P2O5, and K2O) (based on the dry weight of the organic fertilizer) 25%; moisture 0.48%; pH 6.6; density 1.28 g / cm³. 3 .

[0136] Application Example 1

[0137] The yeast-derived bio-organic fertilizer prepared in the examples was added to water, and its effects on dissolved oxygen, ammonia nitrogen removal rate, chlorophyll a content, algal biomass, and algal community structure were tested.

[0138] The experimental pond was divided into eight equally sized areas, named Example 1 Group, Example 2 Group, Example 3 Group, Example 4 Group, Example 5 Group, Example 6 Group, Example 7 Group, and Control Group. Example 1 Group, Example 2 Group, Example 3 Group, Example 4 Group, Example 5 Group, Example 6 Group, and Example 7 Groups were treated with the bio-organic fertilizers prepared in Examples 1, 2, 3, 4, 5, 6, and 7, respectively. The control group received no fertilizer. Except for the control group, the fertilizer application rate was 4.0 kg / mu. The experimental conditions were outdoor natural light and temperature. Day 0 refers to the day after fertilizer application. Weather conditions and water temperature records during the experiment are shown in Table 2 below.

[0139] Table 2 Weather conditions and water temperature records during the experiment.

[0140]

[0141] (1) Dissolved oxygen content in water

[0142] After fertilizer treatment was added to each group of ponds, water samples were taken at 10:00 AM on days 0, 1, 2, 3, 4, 5 and 6 from the middle of the south bank of each pond, 30 cm below the water surface. The dissolved oxygen (DO) content in the water of each group of ponds was analyzed to evaluate the impact of different fertilizers on the aquaculture water quality.

[0143] The dissolved oxygen (DO) content in the water was measured using Hach's LDO standard. TM The dissolved oxygen content (in mg / L) in the water was measured using a portable dissolved oxygen meter, and the data are shown in Table 3 below.

[0144] Table 3 Summary of dissolved oxygen content data in water

[0145]

[0146] Table 4 shows that on the third day after adding the bio-organic fertilizers prepared in Examples 1-7, the dissolved oxygen content in the water was significantly higher than that in the control group. Notably, the compound microbial agents in the bio-organic fertilizers used in Examples 6 and 7 were different from those in Examples 1-5. On the first day after adding the bio-organic fertilizers obtained in Examples 6 and 7, the dissolved oxygen content in the water and its rate of increase over time were lower than those in Examples 1-5. This indicates that the bio-organic fertilizers prepared using the preferred microbial strains have a superior effect on increasing dissolved oxygen in the water.

[0147] (2) Removal of ammonia nitrogen from water

[0148] After fertilizer treatment, water samples were taken from the south bank of each pond at 10:00 AM on days 0, 1, 2, 3, and 4, at a depth of 30 cm below the surface. The ammonia nitrogen (NH3) levels in the water were analyzed. 2+ -N) Main physicochemical factors of water bodies, to evaluate the impact of different fertilizers on aquaculture water quality.

[0149] Ammonia nitrogen is a common harmful substance in aquaculture water, mainly originating from fish metabolic waste and the digestive and metabolic processes of feed. High concentrations can affect the physiological functions and enzyme activity of shrimp, causing metabolic imbalance, inhibiting growth, and reducing disease resistance. Ammonia nitrogen in the aquatic environment contains NH4+. 4+ It exists in two forms: NH3 and non-ionic ammonia. Non-ionic ammonia (NH3) poses a serious threat to aquatic animals, corroding the gill tissue of fish and shrimp and damaging the respiratory function of the gills.

[0150] ammonia nitrogen (NH4+) in water samples 2+The mass concentration (mg / L) of ammonia nitrogen in the water sample was determined according to the People's Republic of China National Environmental Protection Standard HJ535-2009, Determination of Ammonia Nitrogen in Water Quality - Nessler's Reagent Spectrophotometric Method. Then, the ammonia nitrogen removal rate (α1) of the example group was calculated based on the detected concentration of ammonia nitrogen in the water sample. The specific calculation formula is as follows:

[0151]

[0152] In the formula:

[0153] α1 is the removal rate of ammonia nitrogen, expressed as a percentage.

[0154] c 初始 This refers to the initial (i.e., before fertilizer is added) mass concentration of ammonia nitrogen in each group of ponds, expressed in mg / L.

[0155] c n This refers to the mass concentration of ammonia nitrogen in each group of ponds on day n after the addition of fertilizer, expressed in mg / L, where n represents day 1, 2, 3, and 4, respectively.

[0156] △c=cK 初始 -cK n , which is the difference between the initial mass concentration of ammonia nitrogen in the control group pond (before the addition of fertilizer in the example group and the competitor control group) and the mass concentration of ammonia nitrogen on day n (day n after the addition of fertilizer in the example group and the competitor control group), in mg / L.

[0157] The formula for calculating the removal rate (α2) of ammonia nitrogen in the control group is as follows:

[0158]

[0159] In the formula:

[0160] α2 is the removal rate of ammonia nitrogen, expressed as a percentage.

[0161] cK 初始 The initial concentration of ammonia nitrogen in the control group pond (before fertilizer was added to the example group and the competitor's control group) is expressed in mg / L.

[0162] cK n This represents the mass concentration of ammonia nitrogen in the control group pond on day n after the addition of fertilizer, expressed in mg / L, where n represents day 1, 2, 3, and 4.

[0163] The calculated data for the removal rate of ammonia nitrogen in each group are shown in Table 4 below.

[0164] Table 4 Summary data of ammonia nitrogen removal rate

[0165]

[0166] As shown in Table 4, compared with the control group, the addition of fertilizers in Examples 1-7 significantly improved the ammonia nitrogen removal rate. Specifically, after adding the bio-organic fertilizers from Examples 1-5, the ammonia nitrogen removal rate gradually increased over time, with Examples 1-3 achieving an ammonia nitrogen removal rate of over 70% on day 4. However, after adding the bio-organic fertilizers from Examples 6-7, the removal rate initially increased and then decreased over time, indicating that the bio-organic fertilizers prepared in Examples 1-3 were more effective at removing ammonia nitrogen from water.

[0167] (3) Chlorophyll a content

[0168] Chlorophyll is an indispensable substance for plant growth and an important basis for the survival and reproduction of aquatic animals. The chlorophyll content in water directly reflects the growth status of phytoplankton and algae in the water.

[0169] Chlorophyll a content was measured on days 0, 1, 2, 3, 4, and 5 of the experiment. The detection method for chlorophyll a content was referenced in Section 1, Materials and Methods of the literature (Chen Yuwei, Chen Kaining, Hu Yaohui. Discussion on the "Hot Ethanol Method" for the determination of chlorophyll a in phytoplankton and its measurement error [J]. Lake Science, 2006, (05): 550-552). The calculated data of chlorophyll a content in each group are shown in Table 5 below.

[0170] Table 5 Summary of data on changes in chlorophyll a content

[0171]

[0172]

[0173] As shown in Table 5, compared with the control group, the addition of fertilizer in Examples 1-7 inhibited the rate of chlorophyll a decline, and the chlorophyll a content significantly increased starting from day 3. This indicates that the bio-organic fertilizer prepared in these examples is beneficial for increasing the chlorophyll a content in water bodies and promotes the proliferation and growth of algae.

[0174] (4) Types of algae in aquatic bodies

[0175] On the 5th day of the experiment, 500 mL water samples were taken from each group of ponds. After sampling, Lugol's solution (20 g of iodine and 30 g of potassium iodide dissolved in 500 mL of water) was added and allowed to settle for 24 hours. The supernatant was removed by siphon, and the sample was concentrated to 50 mL. Algae were identified and counted using a plankton counting frame, and the cell density of each algae was calculated. If the cell density of a certain algae accounted for 10% or more of the total cell density, then that algae was identified as the dominant species. The dominant algae species in each group are shown in Table 6 below.

[0176] Table 6. Dominant species in each group during the experiment.

[0177]

[0178] As shown in Table 6, compared with the control group, the addition of fertilizer in Examples 1-4 increased the variety of algae. This indicates that the bio-organic fertilizers prepared in Examples 1-4 can significantly promote algae growth in aquaculture water, increase algae abundance, and improve dissolved oxygen conditions in pond water.

[0179] While the specific embodiments of the present invention have been described above, they are not intended to limit the scope of protection of the present invention. Based on the technical solutions of the present invention, various modifications or variations that can be made by those skilled in the art without creative effort are still within the scope of protection of the present invention.

Claims

1. A yeast-derived bio-organic fertilizer suitable for aquaculture, characterized in that, By weight, it comprises: 40-80 parts yeast metabolites, 15-30 parts ammonium chloride, 10-20 parts monoammonium phosphate, and 3-8 parts compound bacterial agent; The compound microbial agent includes Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens; the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens is 3~7: 2~6: 0.5~3.

2. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40-70 parts of yeast metabolites.

3. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40-50 parts of yeast metabolites.

4. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40-42 parts of yeast metabolites.

5. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 15-25 parts of ammonium chloride.

6. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 2, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 15-25 parts of ammonium chloride.

7. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 15-17 parts of ammonium chloride.

8. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture includes 10-15 parts of monoammonium phosphate.

9. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 2, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture includes 10-15 parts of monoammonium phosphate.

10. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 5, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture includes 10-15 parts of monoammonium phosphate.

11. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 10-12 parts of monoammonium phosphate.

12. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 3-6 parts of compound microbial agent.

13. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 2, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 3-6 parts of compound microbial agent.

14. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 5, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 3-6 parts of compound microbial agent.

15. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 8, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 3-6 parts of compound microbial agent.

16. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that, By weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 3-5 parts of compound microbial agent.

17. The yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of claims 1-16, characterized in that, The viable count of the compound microbial agent is 90-110 billion CFU / g.

18. The yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of claims 1-16, characterized in that, The yeast-derived bio-organic fertilizer suitable for aquaculture contains, on a dry basis, a total content of N, P2O5 and K2O of more than 10%.

19. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 17, characterized in that, The yeast-derived bio-organic fertilizer suitable for aquaculture contains, on a dry basis, a total content of N, P2O5 and K2O of more than 10%.

20. The yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of claims 1-16, characterized in that, The pH value is 4.5~8.

5.

21. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 17, characterized in that, The pH value is 4.5~8.

5.

22. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 18, characterized in that, The pH value is 4.5~8.

5.

23. The yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of claims 1-16, characterized in that, Its density is 1.2~1.3 g / cm³. 3 .

24. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 17, characterized in that, Its density is 1.2~1.3 g / cm³. 3 .

25. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 18, characterized in that, Its density is 1.2~1.3 g / cm³. 3 .

26. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 20, characterized in that, Its density is 1.2~1.3 g / cm³. 3 .

27. A method for preparing a yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of claims 1-26, characterized in that, Includes the following steps: Yeast metabolites, ammonium chloride, monoammonium phosphate, and compound bacterial agents are thoroughly mixed to obtain yeast-derived bio-organic fertilizer suitable for aquaculture.

28. The method for preparing yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 27, characterized in that, The yeast metabolites are the metabolic products of yeast extracted through fermentation using molasses or hydrolyzed sugar as a culture medium.

29. The method for preparing yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 27, characterized in that, The yeast metabolites, by weight, comprise 1% to 5% total nitrogen, 11% to 16% potassium, 2% to 8% sodium, 34% to 40% organic matter, 32% to 38% fulvic acid, and 8% to 14% amino acids.

30. The method for preparing yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 28, characterized in that, The yeast metabolites, by weight, comprise 1% to 5% total nitrogen, 11% to 16% potassium, 2% to 8% sodium, 34% to 40% organic matter, 32% to 38% fulvic acid, and 8% to 14% amino acids.

31. A yeast-derived bio-organic fertilizer suitable for aquaculture, characterized in that, The yeast-derived bio-organic fertilizer suitable for aquaculture is prepared by the preparation method of the yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of claims 27-30.

32. The application of a yeast-derived bio-organic fertilizer suitable for aquaculture in the preparation of fertilized water, characterized in that, The yeast-derived bio-organic fertilizer suitable for aquaculture is the yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of claims 1-26 or the yeast-derived bio-organic fertilizer suitable for aquaculture as described in claim 31.

33. The application of a yeast-derived bio-organic fertilizer suitable for aquaculture in reducing ammonia nitrogen content, increasing chlorophyll content, or increasing algae species and content in water, characterized in that, The yeast-derived bio-organic fertilizer suitable for aquaculture is the yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of claims 1-26 or the yeast-derived bio-organic fertilizer suitable for aquaculture as described in claim 31.

34. A fertilizer agent, characterized in that, The fertilizer agent includes the yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of claims 1-26 or the yeast-derived bio-organic fertilizer suitable for aquaculture as described in claim 31.

35. A composition having the effect of reducing ammonia nitrogen content in water, increasing chlorophyll content in water, or increasing the types and content of algae, characterized in that, The composition comprises the yeast-derived bio-organic fertilizer for aquaculture as described in any one of claims 1-26 or the yeast-derived bio-organic fertilizer for aquaculture as described in claim 31.

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