Composite microbial organic fertilizer and preparation method thereof

Abstract

The application discloses a kind of composite microbial organic fertilizer and preparation method thereof, belong to composite microbial organic fertilizer technical field.The composite microbial organic fertilizer of the application is prepared by the following raw materials by weight: microbial inoculum 8-12 parts, livestock and poultry manure 30-35 parts, crop straw 20-25 parts, humic acid 10-15 parts, sugarcane residue charcoal 10-12 parts, over rumen fat powder 3-5 parts, molasses 3-5 parts, chitosan 1-3 parts.The selected azotobacter, streptomyces longisporus and paenibacillus polymyxa in the composite microbial organic fertilizer of the application are compounded, and the three strains are synergized by nitrogen fixation, nutrient activation, hormone secretion and disease inhibition, to comprehensively promote the root development, nutrient absorption and stress resistance of strawberry plants.The composite microbial organic fertilizer of the application can effectively promote the growth of strawberry plants, improve the quality of strawberries and increase the yield, while reducing the residues of chemical fertilizers and pesticides, reducing the risk of soil pollution, and meeting the needs of consumers for safe and organic strawberries.

Description

Technical Field

[0001] The invention belongs to the technical field of compound microbial organic fertilizers, and particularly relates to a compound microbial organic fertilizer and a preparation method thereof. Background Art

[0002] Strawberries are perennial herbs belonging to the genus Fragaria in the Rosaceae family. Their fruits are rich in nutrients such as vitamin C, malic acid, glucose, and citric acid, earning them the nickname "Queen of Fruits." The strawberry industry, with its short production cycle and high economic returns, has become a specialty industry that helps farmers increase their income. As people's quality of life improves, their pursuit of healthy food is increasing, leading to a growing demand for high-quality strawberries.

[0003] Organic fertilizers contain a large number of active substances necessary for plant growth. Applying organic fertilizers promotes healthy plant growth and stress resistance, and can also regulate soil pH. Organic fertilizers are rich in organic matter, and the organic acids produced by their decomposition can further activate mineral elements in the soil, thereby increasing fertilizer utilization. Applying organic fertilizers can also significantly improve fruit quality. Studies have shown that increasing the amount of organic fertilizer used increases the soluble solids, vitamin C, and sugar content of strawberry fruit, while significantly decreasing the titratable acid content. This suggests that applying organic fertilizers can improve strawberry quality. Microbial organic fertilizers are a type of fertilizer composed of a combination of specific functional microorganisms and organic materials. Their core feature is that, through the activity of microorganisms, they decompose organic matter into nutrients that can be absorbed by plants, while also improving the soil microbiome and promoting crop growth.

[0004] For example, Chinese patent application CN201610546465.6 discloses a microbial organic fertilizer specifically for strawberries and a preparation method thereof. The organic fertilizer produced by this invention is specifically for strawberry cultivation. The fermented organic fertilizer is not only odorless but also loose in texture, easy to fertilize, has good water absorption and air permeability, and has a high organic matter content, which can effectively solve the problem of continuous cropping of strawberries. However, this microbial organic fertilizer does not improve the quality of strawberries. Therefore, preparing a bio-organic fertilizer that can provide sufficient nutrition for strawberry plants for a long time, promote the growth of strawberry plants, and improve the quality of strawberry fruits is of great significance for the cultivation of strawberries. Summary of the Invention

[0005] The purpose of the present invention is to provide a composite microbial organic fertilizer that comprehensively promotes root development, nutrient absorption, and stress resistance of strawberry plants and improves fruit quality, while reducing chemical fertilizer and pesticide residues, lowering the risk of soil pollution, and meeting consumers' demand for safe, organic strawberries.

[0006] In order to achieve the above technical objectives, the technical solution adopted by the present invention is:

[0007] The composite microbial organic fertilizer comprises the following raw materials in parts by weight: microbial agent 8-12 parts, livestock and poultry manure 30-35 parts, crop straw 20-25 parts, humic acid 10-15 parts, sugarcane residue charcoal 10-12 parts, over-rumen fat powder 3-5 parts, molasses 3-5 parts, and chitosan 1-3 parts.

[0008] Further, the microbial agent comprises Pseudomonas albuginea, Streptomyces longisporus and Paenibacillus polymyxa; the Pseudomonas albuginea is purchased from the China Center for Type Culture Collection, and has a preservation number of CCTCC S2013531 and an original preservation date of March 28, 2014; the Streptomyces longisporus is purchased from the China Center for Type Culture Collection, and has a preservation number of CCTCC AA94048 and an original preservation date of March 26, 2009; and the Paenibacillus polymyxa is purchased from the China Center for Type Culture Collection, and has a preservation number of CCTCC AB 2013073 and an original preservation date of March 18, 2013.

[0009] Further, the preparation method of the microbial agent is as follows:

[0010] The Pseudomonas albuginea, Streptomyces longisporus and Paenibacillus polymyxa are respectively cultured in a seed culture medium for 48 hours, and then inoculated into an LB culture medium at a 5% inoculation amount, to obtain a medium bacterial liquid when the bacterial concentration OD is 3.0, and then the three kinds of bacterial liquids are mixed uniformly at a volume ratio of 1:1:1, and then freeze-dried to obtain a microbial agent. 600

[0011] Further, the preparation method of the sugarcane residue charcoal is as follows:

[0012] The sugarcane residue is washed with water to remove impurities, and then naturally dried until the water content is less than 15%, and then crushed into small pieces of 3-5 cm, and then placed into a carbonization furnace, and then heated to 400℃ at a rate of 10℃ / min under an oxygen-free condition, and then carbonized for 2 hours, and then cooled by continuing to introduce inert gas after the heating is turned off, to obtain the sugarcane residue charcoal.

[0013] A preparation method of a composite microbial organic fertilizer comprises the following steps:

[0014] (1) Fermentation and maturation: after the livestock and poultry manure and the crop straw are respectively dried with ventilation, they are crushed through a 100-mesh sieve, mixed uniformly in a fermentation tank, and then water is added to control the water content at 50-60%, and then EM bacterial liquid is added, and then the fermentation tank is sealed with a plastic film, and then the fermentation process is performed once every 3 days, and then the fermentation is completed, and then the mixture is spread and dried until the water content is less than or equal to 40%, to obtain fermented organic matter.

[0015] (2) Preparation of microbial agent and sugarcane residue charcoal;

[0016] ​(3) Granulation: the microbial agent prepared in step (2) and bagasse charcoal are uniformly mixed, molasses is added and mixed, and after uniform mixing, the fermented organic matter, humic acid and chitosan oligosaccharide prepared in step (1) are added, and the mixture is stirred and mixed uniformly, and the mixture is dried to a moisture content of ≤15%. The mixture is granulated by a granulator, and particles with a particle size of 3-5 mm are screened out. The particles are placed in a spiral mixer and stirred at a speed of 20-30 r / min. During the stirring process, rumen-passed fat powder is evenly sprayed onto the particle surface through a sprayer, and stirring is continued for 10 minutes to make the fat powder evenly distributed on the particle surface. The particles are spread out and naturally cooled to obtain the final product, composite microbial organic fertilizer particles.

[0017] Furthermore, the livestock manure is pig manure; the crop straw is peanut straw; and the added amount of the EM bacterial solution is 0.1% of the total weight of the manure and straw.

[0018] Furthermore, the composite microbial organic fertilizer of the present invention is used in strawberry planting.

[0019] The raw materials used in the present invention are all commercially available.

[0020] The nitrogen-fixing Pseudomonas aeruginosa of the present invention converts atmospheric nitrogen into ammonium nitrogen through biological nitrogen fixation, directly supplementing the nitrogen demand of strawberries. During the strawberry's vegetative growth period, when nitrogen requirements are high, this bacterium can reduce nitrogen fertilizer input by 30-50% while also preventing soil acidification caused by chemical nitrogen fertilizers. Furthermore, it secretes the plant hormone indoleacetic acid, which promotes root development and increases root surface area and absorptive capacity. Paenibacillus polymyxa has phosphate and potassium solubilizing properties and secretes organic acids and phosphatases, releasing fixed phosphorus in the soil. The siderophores it produces can chelate insoluble potassium, converting it into absorbable forms, improving the strawberry's utilization of mineral nutrients. It also produces indoleacetic acid. Streptomyces longisporus secretes antibiotics, such as actinomycin, to inhibit gray mold pathogens, reducing the incidence of strawberry diseases. It also regulates the soil microbiome and reduces the risk of soil-borne diseases. The combined action of these three bacteria can enhance strawberries' resistance to root rot and promote their growth.

[0021] The present invention adds humic acid during the preparation of organic fertilizer. Humic acid and microbial metabolites can strengthen the cell wall structure, increase fruit firmness, and prevent surface damage during strawberry transportation. The present invention utilizes peanut straw and pig manure for fermentation, utilizing agricultural waste as a resource, reducing environmental pollution and lowering fertilizer costs. Furthermore, the composted corn straw releases large amounts of nitrogen, phosphorus, and potassium, particularly potassium, which is crucial for strawberry fruit enlargement and sugar accumulation.

[0022] During the preparation of the organic fertilizer of the present invention, rumen bypass fat powder and molasses are added. Rumen bypass fat powder has the property of resisting decomposition, reduces the excessive consumption of microorganisms during the fertilizer processing process, provides a long-acting carbon source, continuously supports microbial activity, avoids the rapid release of nutrients, and prolongs the fertilizer effect. In addition, rumen bypass fat powder forms a hydrophobic protective film on the surface of the particles, reduces moisture absorption and agglomeration of organic fertilizer particles during storage and transportation, reduces the loss of volatile nutrients such as nitrogen and potassium, and improves nutrient utilization. Rumen bypass fat powder slowly decomposes in the soil, releases fatty acids after decomposition, improves the soil aggregate structure, and enhances air permeability and water retention. Molasses is rich in carbon sources such as sucrose and glucose, which activates microbial agents and ensures their normal survival and value-added. In order to improve the stability of microorganisms, bagasse charcoal is prepared, and the pores inside the biochar are used to achieve adsorption and protection of microorganisms.

[0023] Beneficial effects

[0024] The composite microbial organic fertilizer of the present invention is compounded with three strains of nitrogen-fixing Pseudomonas, Streptomyces longisporus, and Paenibacillus polymyxa. The three strains comprehensively promote the root development, nutrient absorption, and stress resistance of strawberry plants and improve the quality of the fruit through the synergistic effects of nitrogen fixation, nutrient activation, hormone secretion, disease inhibition, and microbiome regulation.

[0025] The composite microbial organic fertilizer of the present invention significantly improves the fertility of the strawberry rhizosphere soil, improves the quality and yield of strawberries, and at the same time reduces chemical fertilizer and pesticide residues, lowers the risk of soil pollution, and meets consumers' demand for safe and organic strawberries. DETAILED DESCRIPTION

[0026] The technical solution of the present invention will be further described below with reference to specific embodiments, but is not limited thereto.

[0027] Example 1

[0028] A composite microbial organic fertilizer comprises the following raw materials in parts by weight: 8 parts of microbial agents, 30 parts of livestock and poultry manure, 20 parts of crop straws, 10 parts of humic acid, 10 parts of bagasse charcoal, 3 parts of rumen bypass fat powder, 3 parts of molasses, and 1 part of chitosan oligosaccharide.

[0029] The microbial agent includes Pseudomonas azotobacter, Streptomyces longisporus, and Bacillus polymyxa; the Pseudomonas azotobacter was purchased from the China Center for Type Culture Collection with a deposit number of CCTCC S2013531 and an original deposit date of March 28, 2014; the Streptomyces longisporus was purchased from the China Center for Type Culture Collection with a deposit number of CCTCC AA 94048 and an original deposit date of March 26, 2009; the Bacillus polymyxa was purchased from the China Center for Type Culture Collection with a deposit number of CCTCC AB 2013073 and an original deposit date of March 18, 2013.

[0030] The preparation method of the microbial agent is as follows:

[0031] Pseudomonas azotobacter, Streptomyces longisporus and Paenibacillus polymyxa were cultured in seed culture medium for 48 h, and then inoculated into LB culture medium at a 5% inoculum volume to cultivate until the bacterial concentration OD 600 =3.0 to obtain the intermediate bacterial solution, and then the three bacterial solutions were evenly mixed in a volume ratio of 1:1:1, and freeze-dried into freeze-dried powder to obtain the microbial agent.

[0032] The preparation method of the bagasse charcoal is:

[0033] The sugarcane bagasse is rinsed with water to remove impurities, and naturally aired until the moisture content is less than 15%. The dried sugarcane bagasse is broken into small pieces of 3-5 cm, and the sugarcane bagasse is placed in a carbonization furnace. Under anaerobic conditions, the temperature is raised to 400°C at a rate of 10°C / min, carbonized for 2 hours, the heating is turned off, and inert gas is continued to be introduced for cooling to obtain sugarcane bagasse charcoal.

[0034] A method for preparing a composite microbial organic fertilizer comprises the following steps:

[0035] (1) Fermentation and composting: After the livestock manure and crop straw are ventilated and dried, they are crushed and passed through a 100-mesh sieve. After being placed in a fermentation tank, they are mixed evenly. Water is added to control the moisture content to 50-60%. EM bacterial solution is then added. The fermentation tank is sealed with a plastic film. The fermentation process is turned over once every three days until the composting is completed. The piles are then spread out and aired until the moisture content is ≤40% to obtain fermented organic matter.

[0036] (2) preparing microbial agents and bagasse charcoal;

[0037] (3) Granulation: the microbial agent prepared in step (2) and bagasse charcoal are uniformly mixed, molasses is added and mixed, and after uniform mixing, the fermented organic matter, humic acid and chitosan oligosaccharide prepared in step (1) are added, and the mixture is stirred and mixed uniformly, and the mixture is dried to a moisture content of ≤15%. The mixture is granulated by a granulator, and particles with a particle size of 3-5 mm are screened out. The particles are placed in a spiral mixer and stirred at a speed of 20-30 r / min. During the stirring process, rumen-passed fat powder is evenly sprayed onto the particle surface through a sprayer, and stirring is continued for 10 minutes to make the fat powder evenly distributed on the particle surface. The particles are spread out and naturally cooled to obtain the final product, composite microbial organic fertilizer particles.

[0038] The livestock excrement is pig excrement; the crop straw is peanut straw; and the added amount of the EM bacterial solution is 0.1% of the total weight of the excrement and straw.

[0039] Example 2

[0040] A compound microbial organic fertilizer comprises the following raw materials in parts by weight: 10 parts of microbial agent, 32 parts of livestock and poultry manure, 23 parts of crop straw, 13 parts of humic acid, 11 parts of bagasse charcoal, 4 parts of rumen bypass fat powder, 4 parts of molasses, and 2 parts of chitosan oligosaccharide.

[0041] The microbial agent includes Pseudomonas azotobacter, Streptomyces longisporus, and Bacillus polymyxa; the Pseudomonas azotobacter was purchased from the China Center for Type Culture Collection with a deposit number of CCTCC S2013531 and an original deposit date of March 28, 2014; the Streptomyces longisporus was purchased from the China Center for Type Culture Collection with a deposit number of CCTCC AA 94048 and an original deposit date of March 26, 2009; the Bacillus polymyxa was purchased from the China Center for Type Culture Collection with a deposit number of CCTCC AB 2013073 and an original deposit date of March 18, 2013.

[0042] The preparation method of the microbial agent is as follows:

[0043] Pseudomonas azotobacter, Streptomyces longisporus and Paenibacillus polymyxa were cultured in seed culture medium for 48 h, and then inoculated into LB culture medium at a 5% inoculum volume to cultivate until the bacterial concentration OD 600 =3.0 to obtain the intermediate bacterial solution, and then the three bacterial solutions were evenly mixed in a volume ratio of 1:1:1, and freeze-dried into freeze-dried powder to obtain the microbial agent.

[0044] The preparation method of the bagasse charcoal is:

[0045] The sugarcane bagasse is rinsed with water to remove impurities, and naturally aired until the moisture content is less than 15%. The dried sugarcane bagasse is broken into small pieces of 3-5 cm, and the sugarcane bagasse is placed in a carbonization furnace. Under anaerobic conditions, the temperature is raised to 400°C at a rate of 10°C / min, carbonized for 2 hours, the heating is turned off, and inert gas is continued to be introduced for cooling to obtain sugarcane bagasse charcoal.

[0046] A method for preparing a composite microbial organic fertilizer comprises the following steps:

[0047] (1) Fermentation and composting: After the livestock manure and crop straw are ventilated and dried, they are crushed and passed through a 100-mesh sieve. After being placed in a fermentation tank, they are mixed evenly. Water is added to control the moisture content to 50-60%. EM bacterial solution is then added. The fermentation tank is sealed with a plastic film. The fermentation process is turned over once every three days until the composting is completed. The piles are then spread out and aired until the moisture content is ≤40% to obtain fermented organic matter.

[0048] (2) preparing microbial agents and bagasse charcoal;

[0049] (3) Granulation: the microbial agent prepared in step (2) and bagasse charcoal are uniformly mixed, molasses is added and mixed, and after uniform mixing, the fermented organic matter, humic acid and chitosan oligosaccharide prepared in step (1) are added, and the mixture is stirred and mixed uniformly, and the mixture is dried to a moisture content of ≤15%. The mixture is granulated by a granulator, and particles with a particle size of 3-5 mm are screened out. The particles are placed in a spiral mixer and stirred at a speed of 20-30 r / min. During the stirring process, rumen-passed fat powder is evenly sprayed onto the particle surface through a sprayer, and stirring is continued for 10 minutes to make the fat powder evenly distributed on the particle surface. The particles are spread out and naturally cooled to obtain the final product, composite microbial organic fertilizer particles.

[0050] The livestock excrement is pig excrement; the crop straw is peanut straw; and the added amount of the EM bacterial solution is 0.1% of the total weight of the excrement and straw.

[0051] Example 3

[0052] A composite microbial organic fertilizer comprises the following raw materials in parts by weight: 12 parts of microbial agent, 35 parts of livestock and poultry manure, 25 parts of crop straw, 15 parts of humic acid, 12 parts of bagasse charcoal, 5 parts of rumen bypass fat powder, 5 parts of molasses, and 3 parts of chitosan oligosaccharide.

[0053] The microbial agent includes Pseudomonas azotobacter, Streptomyces longisporus, and Bacillus polymyxa; the Pseudomonas azotobacter was purchased from the China Center for Type Culture Collection with a deposit number of CCTCC S2013531 and an original deposit date of March 28, 2014; the Streptomyces longisporus was purchased from the China Center for Type Culture Collection with a deposit number of CCTCC AA 94048 and an original deposit date of March 26, 2009; the Bacillus polymyxa was purchased from the China Center for Type Culture Collection with a deposit number of CCTCC AB 2013073 and an original deposit date of March 18, 2013.

[0054] The preparation method of the microbial agent is as follows:

[0055] Pseudomonas azotobacter, Streptomyces longisporus and Paenibacillus polymyxa were cultured in seed culture medium for 48 h, and then inoculated into LB culture medium at a 5% inoculum volume to cultivate until the bacterial concentration OD 600 =3.0 to obtain the intermediate bacterial solution, and then the three bacterial solutions were evenly mixed in a volume ratio of 1:1:1, and freeze-dried into freeze-dried powder to obtain the microbial agent.

[0056] The preparation method of the bagasse charcoal is:

[0057] The sugarcane bagasse is rinsed with water to remove impurities, and naturally aired until the moisture content is less than 15%. The dried sugarcane bagasse is broken into small pieces of 3-5 cm, and the sugarcane bagasse is placed in a carbonization furnace. Under anaerobic conditions, the temperature is raised to 400°C at a rate of 10°C / min, carbonized for 2 hours, the heating is turned off, and inert gas is continued to be introduced for cooling to obtain sugarcane bagasse charcoal.

[0058] A method for preparing a composite microbial organic fertilizer comprises the following steps:

[0059] (1) Fermentation and composting: After the livestock manure and crop straw are ventilated and dried, they are crushed and passed through a 100-mesh sieve. After being placed in a fermentation tank, they are mixed evenly. Water is added to control the moisture content to 50-60%. EM bacterial solution is then added. The fermentation tank is sealed with a plastic film. The fermentation process is turned over once every three days until the composting is completed. The piles are then spread out and aired until the moisture content is ≤40% to obtain fermented organic matter.

[0060] (2) preparing microbial agents and bagasse charcoal;

[0061] (3) Granulation: the microbial agent prepared in step (2) and bagasse charcoal are uniformly mixed, molasses is added and mixed, and after uniform mixing, the fermented organic matter, humic acid and chitosan oligosaccharide prepared in step (1) are added, and the mixture is stirred and mixed uniformly, and the mixture is dried to a moisture content of ≤15%. The mixture is granulated by a granulator, and particles with a particle size of 3-5 mm are screened out. The particles are placed in a spiral mixer and stirred at a speed of 20-30 r / min. During the stirring process, rumen-passed fat powder is evenly sprayed onto the particle surface through a sprayer, and stirring is continued for 10 minutes to make the fat powder evenly distributed on the particle surface. The particles are spread out and naturally cooled to obtain the final product, composite microbial organic fertilizer particles.

[0062] The livestock excrement is pig excrement; the crop straw is peanut straw; and the added amount of the EM bacterial solution is 0.1% of the total weight of the excrement and straw.

[0063] Comparative Example

[0064] In each comparative example, the volume ratio of Pseudomonas azotobacter, Streptomyces longisporus, and Paenibacillus polymyxa in the microbial agent was changed, and the remaining raw materials and steps were the same as those in Example 3.

[0065] Table 1 Composition of bacterial solutions in each comparative example

[0066] Group Components Azotobacter aeruginosa(a) Streptomyces longisporus (b) Paenibacillus polymyxa (c) Comparative Example 1 a+b+c 1 2 1 Comparative Example 2 a+b+c 1 1 2 Comparative Example 3 a+b+c 2 1 1 Comparative Example 4 a+b 1 1 0 Comparative Example 5 a+c 1 0 1 Comparative Example 6 b+c 0 1 1 Comparative Example 7 a 1 0 0 Comparative Example 8 b 0 1 0 Comparative Example 9 c 0 0 1

[0067] Performance Testing

[0068] Identification of strain growth-promoting ability

[0069] The phosphate solubilization ability of the strain was determined by the molybdenum antimony colorimetric method; the potassium solubilization ability of the strain was determined by the flame atomic spectrophotometer; the nitrogen fixation ability of the strain was determined by the carbon-nitrogen analyzer; the indoleacetic acid secretion ability of the strain was determined by the Salkowski colorimetric solution method; and the siderophore synthesis ability of the strain was determined by the CAS liquid colorimetric method.

[0070] Table 2 Identification of growth-promoting ability of strains

[0071]

[0072] Verification of the antibacterial effect of microbial liquid:

[0073] Pathogen inhibition test: Using the plate standoff method, strawberry gray mold and root rot pathogens were activated and cultured on PDA plates. A 5 mm cake of each pathogen was placed in the center of a new PDA plate. Two sterile filter paper discs were placed approximately 2.5 cm from the center of the plate. 10 μl of the mixed microbial solution from Example 3 was dripped onto each filter paper disc. LB liquid broth was added as a blank control. Each treatment was repeated six times, incubated at 25°C. After three days of incubation, the diameters of the pathogen colonies were measured, and the values ​​were averaged to calculate the inhibition rate. The data are shown in Table 3.

[0074] Table 3 Antibacterial effect of microbial liquid

[0075] pathogens Control group (mm) Colony diameter of treatment group (mm) Antibacterial rate (%) Botrytis cinerea 68 32 57.1 Rhizoctonia solani 59 24 64.8

[0076] As shown in Table 3, the mixed bacterial liquid of the three microorganisms selected in the present invention has a good antagonistic effect on strawberry gray mold and root rot pathogens.

[0077] Planting trials

[0078] The planting experiment was conducted in a plastic greenhouse at our company's test base. The strawberry variety tested was Zhang Ji. The experiment set up 13 treatment groups, which were respectively applied with the microbial organic fertilizers of Examples 1-3 and Comparative Examples 1-9. Another group was not applied with any fertilizer. Each treatment group had 100m 2 The plots were arranged in randomized blocks, with each treatment receiving 600 kg / mu of fertilizer. Fertilizer was evenly spread before ridging. High ridge cultivation was used, with a ridge width of 60 cm, a ridge height of 30 cm, and a furrow width of 30 cm. Two rows were planted per ridge, with a spacing of 18 cm between plants. Bees were used for pollination during flowering, and other management measures were the same as those for conventional field management.

[0079] Four months after strawberry planting, 10 strawberry plants were selected from each treatment group to measure plant height and width. During the fruit harvest period, mature fruits from 10 plants were sampled using a five-point sampling method. The longitudinal and transverse diameters, fruit weight, and yield per plant were measured. These data are shown in Table 4. The soluble solids content of the harvested fruits was measured using a refractometer, and the fruit firmness was measured using a fruit hardness meter. The results are shown in Table 5.

[0080] Table 4 Plant and fruit characteristics of strawberries under different treatments

[0081] Group Plant height / cm Plant width / cm Fruit longitudinal diameter / cm Fruit diameter / cm Average single fruit weight / g Average yield per plant / g Example 1 22.8 35.6 4.98 3.04 17.2 214.4 Example 2 23.1 35.8 5.04 3.07 17.3 218.5 Example 3 23.3 36.1 5.06 3.11 17.6 223.7 Comparative Example 1 22.0 34.2 4.84 2.93 16.8 205.3 Comparative Example 2 22.4 34.7 4.92 3.02 17.1 212.5 Comparative Example 3 22.5 34.6 4.87 2.98 16.9 208.9 Comparative Example 4 20.8 33.1 4.65 2.82 15.7 185.6 Comparative Example 5 21.7 33.9 4.83 2.88 16.3 201.4 Comparative Example 6 21.3 33.5 4.76 2.85 16.0 196.2 Comparative Example 7 19.8 32.6 4.59 2.79 15.2 173.9 Comparative Example 8 19.1 32.1 4.47 2.75 14.9 167.1 Comparative Example 9 20.5 32.8 4.61 2.86 15.5 181.8 control group 17.6 31.4 4.35 2.72 14.6 161.5

[0082] As shown in Table 4, compared with the blank, after applying the composite microbial organic fertilizer prepared by Examples 1-3 of the present invention, the strawberry plant growth is more luxuriant, the fruit is larger and fuller, and the strawberry individual plant yield is also significantly improved. The comparative examples 1-9 that change the composition of the microbial agent all have varying degrees of decline in the data of strawberry plant traits and fruit traits, indicating that the three selected bacteria in the composite microbial inoculum of the present invention are less effective if one is missing, and each bacterium is indispensable for the preparation of the composite microbial organic fertilizer of the present invention.

[0083] Table 5 Fruit quality traits under different treatments

[0084]

[0085]

[0086] As shown in Table 5, the application of the composite microbial organic fertilizers of Examples 1-3 of the present invention significantly increased the soluble solids content of strawberry fruit, with a small difference in the soluble solids content between the fruit tip and the flesh. The flesh was sweeter and more flavorful, while the fruit firmness was improved. However, after applying the organic fertilizers of Comparative Examples 1-9, which varied the bacterial composition of the microbial inoculants, the soluble solids content and firmness of the strawberry fruit decreased to varying degrees, resulting in a significant decline in the quality of the strawberry fruit.

[0087] It should be noted that the above embodiments are only some of the preferred embodiments of the present invention, and not all of them. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of the present invention.

Claims

1. A composite microbial organic fertilizer, characterized in that: The invention comprises the following raw materials in parts by weight: 8-12 parts of microbial agent, 30-35 parts of livestock and poultry manure, 20-25 parts of crop straw, 10-15 parts of humic acid, 10-12 parts of bagasse charcoal, 3-5 parts of rumen bypass fat powder, 3-5 parts of molasses, and 1-3 parts of chitosan oligosaccharide; The microbial agent includes nitrogen-fixing Pseudomonas, Streptomyces longisporus, and Paenibacillus polymyxa; the deposit number of the nitrogen-fixing Pseudomonas is CCTCC S2013531; the deposit number of the Streptomyces longisporus is CCTCC AA 94048; and the deposit number of Paenibacillus polymyxa is CCTCC AB 2013073; the preparation method of the microbial agent is: nitrogen-fixing Pseudomonas, Streptomyces longisporus, and Paenibacillus polymyxa are cultured in a seed culture medium for 48 hours, and then inoculated into LB culture medium at an inoculum size of 5%, and cultured until the bacterial concentration OD 600 =3.0 to obtain the medium bacterial solution, then mix the three bacterial solutions in a volume ratio of 1:1:1, freeze-dry into freeze-dried powder, and obtain the microbial agent; The composite microbial organic fertilizer is used for strawberry planting.

2. according to the described compound microbial organic fertilizer of claim 1, it is characterized in that, The preparation method of the bagasse charcoal is: The sugarcane bagasse is rinsed with water to remove impurities, and naturally aired until the moisture content is less than 15%. The dried sugarcane bagasse is broken into small pieces of 3-5 cm, and the sugarcane bagasse is placed in a carbonization furnace. Under anaerobic conditions, the temperature is raised to 400°C at a rate of 10°C / min, carbonized for 2 hours, the heating is turned off, and inert gas is continued to be introduced for cooling to obtain sugarcane bagasse charcoal.

3. A method for preparing the composite microbial organic fertilizer according to any one of claims 1-2, characterized in that: The following steps are involved: (1) Fermentation and composting: After the livestock manure and crop straw are ventilated and dried, they are crushed through a 100-mesh sieve, placed in a fermentation tank and mixed evenly. Water is added to control the moisture content to 50-60%, and then EM bacterial solution is added. The fermentation tank is sealed with plastic film. The fermentation process is turned over once every 3 days until the composting is completed. The fermented organic matter is then spread out and aired until the moisture content is ≤40% to obtain fermented organic matter. (2) preparing microbial agents and bagasse charcoal; (3) Granulation: the microbial agent prepared in step (2) and bagasse charcoal are uniformly mixed, molasses is added and mixed, and after uniform mixing, the fermented organic matter, humic acid and chitosan oligosaccharide prepared in step (1) are added, and the mixture is stirred and mixed uniformly, and the mixture is dried to a moisture content of ≤15%. The mixture is granulated by a granulator, and particles with a particle size of 3-5 mm are screened out. The particles are placed in a spiral mixer and stirred at a speed of 20-30 r / min. During the stirring process, rumen-passed fat powder is evenly sprayed onto the particle surface through a sprayer, and stirring is continued for 10 min to make the fat powder evenly distributed. The particles are spread out and naturally cooled to obtain composite microbial organic fertilizer particles.

4. The preparation method of compound microbial organic fertilizer according to claim 3, wherein The livestock excrement is pig excrement; the crop straw is peanut straw; and the added amount of the EM bacterial solution is 0.1% of the total weight of the excrement and straw.

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