Purification and separation device and method for hericium erinaceus polysaccharide extraction

Abstract

The invention relates to the technical field of polysaccharide processing, and discloses a hericium erinaceus polysaccharide extraction purification and separation device which comprises a fixed base, a rotating base is connected to the top end of the fixed base, a driving part is connected to the bottom end of the inner wall of the fixed base, and the output end of the driving part penetrates through the fixed base and is connected with the rotating base. According to the device disclosed by the invention, the decoloration bin and the deproteinization bin are connected, so that two steps of decoloration and deproteinization can be directly connected, and impurities and precipitates in an extracting solution can be effectively and quickly filtered out through an internal filtering mechanism, so that the purification effect of polysaccharide extraction is ensured; the driving part can synchronously perform centrifugal operation on extracting solutions in the decolorizing bin, the deproteinization bin and the extraction bin, so that the operation steps are reduced, the balancing weight at the top plays a role in adjusting balance, and the device is adsorbed on a placement base by utilizing the positioning suction cup, so that the use stability of the device is effectively ensured, the processing time is shortened, and the processing efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of polysaccharide processing, and specifically to a purification and separation device and method for extracting Hericium erinaceus polysaccharide. Background Art

[0002] Hericium erinaceus polysaccharide refers to a polysaccharide compound extracted from the fruit body of Hericium erinaceus. The main components of Hericium erinaceus polysaccharide include β-glucan (such as β-1,3-glucan and β-1,6-glucan), protein, and some other types of polysaccharides, and these polysaccharides have various biological activities.

[0003] Currently, after the preliminary extraction of Hericium erinaceus polysaccharide, a series of operations such as decolorization and deproteinization are required to further improve the purity of the polysaccharide. Each step of the operation requires a long time-consuming and a large number of operating instruments to be used in cooperation, which greatly increases the production cost and production time of purification, and reduces the processing efficiency.

[0004] The above information disclosed in this background art is only used to increase the understanding of the background art of this application. Therefore, it may include prior art that is not known to those of ordinary skill in the art. Summary of the Invention

[0005] The purpose of the present invention is to provide a purification and separation device and method for extracting Hericium erinaceus polysaccharide to solve the problems raised in the above background art.

[0006] To achieve the above object, the present invention provides the following technical solutions: It includes a fixed base, a rotating base is connected and arranged at the top end of the fixed base, a driving member is connected and arranged at the bottom end of the inner wall of the fixed base, the output end of the driving member penetrates through the fixed base and is connected with the rotating base, a plurality of positioning suction cups are connected and arranged at the bottom end of the inner wall of the fixed base, two fixed side plates are connected and arranged at the top end of the rotating base, rotating shafts are rotatably arranged on the sides of the fixed side plates close to each other, a connecting frame is connected and arranged at the ends of the rotating shafts close to each other, a dehydration bin is connected and arranged inside the connecting frame, fixed frames are connected and arranged on both sides of the connecting frame, a top plate is connected and arranged at the top end of the fixed frame, a pushing cylinder is connected and arranged at the top end of the top plate, the output end of the pushing cylinder penetrates through the top plate and is connected with an extrusion and mixing mechanism, a servo motor is connected and arranged at the top end of the top plate, a plurality of connecting cylinders are connected and arranged on the surface of the dehydration bin, a deproteinization bin is connected and arranged at the output end of the connecting cylinder, electric heating layers are connected and arranged on the outer surfaces of the deproteinization bin and the dehydration bin, a driving shaft is connected and arranged at the output end of the servo motor, the bottom end of the driving shaft penetrates through the extrusion and mixing mechanism and the dehydration bin and is rotatably arranged at the bottom end of the inner wall of the deproteinization bin, filtering mechanisms are connected and arranged in the dehydration bin and the deproteinization bin in a threaded manner, threads are provided on the inner walls of the dehydration bin and the deproteinization bin corresponding to the filtering mechanisms, connecting pipes are connected and arranged on both sides of the deproteinization bin, and the other ends of the connecting pipes are connected and arranged with an extraction bin; A water filtering mechanism is connected and arranged inside the extraction bin, a liquid discharging unit is connected and arranged inside the water filtering mechanism, a storage box is connected and arranged at the bottom end of the extraction bin, the ends of the rotating shafts far from each other penetrate through the corresponding fixed side plates and are connected with swing frames, an inclined plate is connected and arranged at the top end of the swing frame, a counterweight block is fixedly arranged at the top end of the inclined plate, a telescopic rod is rotatably arranged on one side of the swing frame, and the other end of the telescopic rod is rotatably arranged on one side of the fixed side plate.

[0007] Preferably, rectangular strips are connected and arranged on both sides of the driving shaft inside the dehydration bin and the deproteinization bin, and the rectangular strips are slidably connected with the filtering mechanism and the extrusion and mixing mechanism.

[0008] Preferably, an annular limiting groove is opened at the top end of the extrusion and mixing mechanism, a T-shaped arc block is connected and arranged at the bottom end of the pushing cylinder and is slidably connected with the annular limiting groove.

[0009] Preferably, the extrusion and mixing mechanism includes a movable cover plate slidably arranged in the dehydration bin, a rotating frame is rotatably arranged at the top end of the movable cover plate, a stirring plate is connected and arranged at the bottom end of the rotating frame, a rotating cylinder is connected and arranged on one side of the rotating frame, and the stirring plate is fitted with the movable cover plate.

[0010] Preferably, the filtering mechanism includes a filtering frame. A first filter layer is connected and arranged at the top end of the filtering frame. A sealing shell is connected and arranged at the bottom end of the filtering frame. An electric push rod is connected and arranged inside the sealing shell. The output end of the electric push rod penetrates through the sealing shell and is connected and arranged with an arc-shaped threaded plate. A second filter layer is connected and arranged at the bottom end of the filtering frame.

[0011] Preferably, a connecting frame is connected and arranged at the bottom end of the filtering frame located in the dehydration bin. A sealing plug is rotatably arranged at the bottom end of the connecting frame. The bottom end of the dehydration bin is provided with a conical protruding end corresponding to the sealing plug. The top end of the deproteinization bin is provided with a conical concave end corresponding to the conical protruding end. And an extrusion plate is connected and arranged at the bottom end of the concave end. A medicine injection pipe is connected and arranged at the top end of the extrusion plate.

[0012] Preferably, the water filtering mechanism includes a collecting tank. A wire mesh frame is connected and arranged on the outer side of the collecting tank. A counterweight shell is connected and arranged on the outer side of the wire mesh frame. An air bag is connected and arranged inside the counterweight shell. A third filter layer is connected and arranged at the bottom end of the wire mesh frame. The liquid discharging unit is connected and arranged in the collecting tank. A plurality of liquid seepage openings are formed on the outer surface of the collecting tank.

[0013] Preferably, the liquid discharging unit includes a baffle fixedly arranged on the inner side of the collecting tank. An extraction pump is connected and arranged at the top end of the baffle. The water inlet end of the extraction pump extends below the baffle and is connected and arranged with a washing liquid cover. The water outlet end of the extraction pump is connected and arranged with a liquid discharging pipe.

[0014] Preferably, a guide rod is connected and arranged on one side of the inner wall of the extraction bin. A fitting groove corresponding to the guide rod is formed in the outer ring of the counterweight shell.

[0015] Preferably, a method for a purification and separation device for extracting Hericium erinaceus polysaccharide includes the following steps: (1) Decolorization treatment: Inject activated carbon powder and the polysaccharide extraction solution preliminarily extracted from Hericium erinaceus into the dehydration bin. Then push the air cylinder to extend and drive the extrusion and mixing mechanism to enter the top end of the dehydration bin, thereby sealing the dehydration bin. Then rotate the air cylinder to drive the rotating frame to rotate, so that the stirring plate rotates and extends into the dehydration bin. Start the servo motor, drive the movable cover plate to rotate through the drive shaft, use the stirring plate to stir the extraction solution, and at the same time, the electric heating layer outside the dehydration bin heats the extraction solution in the dehydration bin to improve the mixing effect. After mixing is completed, push the air cylinder to lift the extrusion and mixing mechanism, then retract the stirring plate. At the same time, the electric push rod in the filtering mechanism in the dehydration bin extends, pushes the arc-shaped threaded plate to be threadedly connected with the inner wall of the dehydration bin, and then as the filtering frame rotates, drives the whole filtering mechanism to rise, and filters the activated carbon powder adsorbed with pigment in the extraction solution through the first filter layer, the filtering frame and the second filter layer; (2) Deproteinization treatment: The decolorized extract is discharged into the deproteinization tank, and then the corresponding protease is injected into the deproteinization tank through the medicine injection pipe. During the injection process, it is mixed with the decolorized extract. At this time, the telescopic rod can be activated to extend and push the swing frame to rotate, thereby driving the connecting frame to rotate through the rotating shaft, so that the dehydration tank and the deproteinization tank are tilted. Then, the driving member is activated to drive the rotating base to rotate, and centrifugation operation is performed on the mixed solution in the deproteinization tank. After centrifugation, the filtering mechanism in the deproteinization tank is also activated and rises with rotation to filter out the protein precipitate in the solution. Then, the feeding pump pumps out the filtered solution. During the pumping process, negative pressure can also be created in the deproteinization tank to improve the filtering effect, and the solution is discharged into the extraction tank; (3) Polysaccharide extraction: After injecting the corresponding purified polysaccharide solution into the extraction tank, ethanol or methanol is injected to precipitate the polysaccharide inside. Then, the water filtering mechanism is placed in the extraction tank. As the water filtering mechanism sinks, the third filter layer filters out the liquid. The liquid passes through the wire mesh frame and then enters the collection tank through the liquid seepage port. The airbag can prevent the water filtering mechanism from sinking too fast. After the liquid enters the collection tank, the extraction pump is turned on, and the liquid is pumped out through the washing liquid cover and discharged through the drain pipe until the liquid is finally squeezed out. Finally, the storage box is removed, and the purified polysaccharide is obtained.

[0016] In summary, the present application includes the following beneficial technical effects: Through the connection setting between the decolorization tank and the deproteinization tank, the two steps of decolorization and deproteinization can be directly connected. At the same time, the internal filtering mechanism can effectively and quickly filter out the impurities and precipitates in the extract, ensuring the purification effect of polysaccharide extraction. As the operation progresses, multiple steps can be carried out simultaneously. The driving member can perform centrifugation operations on the extracts in the decolorization tank, deproteinization tank, and extraction tank synchronously, reducing the operation steps. The counterweight block at the top plays a role in adjusting the balance, and it is adsorbed on the placement base by the positioning suction cup, effectively ensuring the stability of the device during use, reducing the operation of personnel, and at the same time reducing the processing time-consuming and greatly improving the processing efficiency. Description of the Drawings

[0017] Figure 1 is the overall structural schematic diagram of a purification and separation device for extracting Hericium erinaceus polysaccharide according to the present invention; Figure 2 is the side-sectional structural schematic diagram of a purification and separation device for extracting Hericium erinaceus polysaccharide according to the present invention; Figure 3 is Figure 2 the enlarged structural schematic diagram of part A in Figure 4 is the structural schematic diagram of the water filtering mechanism in a purification and separation device for extracting Hericium erinaceus polysaccharide according to the present invention; Figure 5This is a schematic structural diagram of the filtering mechanism in a purification and separation device for extracting Hericium erinaceus polysaccharide according to the present invention; Figure 6 This is a partial structural schematic diagram of a purification and separation device for extracting Hericium erinaceus polysaccharide according to the present invention; Figure 7 This is a schematic structural diagram of the extrusion and mixing mechanism in a purification and separation device for extracting Hericium erinaceus polysaccharide according to the present invention.

[0018] In the figure: 1, fixed base; 2, rotating base; 3, driving member; 4, positioning suction cup; 5, fixed side plate; 6, rotating shaft; 7, connecting frame; 8, dehydration bin; 9, fixing frame; 10, top plate; 11, pushing cylinder; 12, extrusion and mixing mechanism; 121, movable cover plate; 122, rotating frame; 123, stirring plate; 124, rotating cylinder; 13, servo motor; 14, connecting cylinder; 15, deproteinization bin; 16, electric heating layer; 18, driving shaft; 19, filtering mechanism; 191, filter frame; 192, first filter layer; 193, sealing shell; 194, electric push rod; 195, arc-shaped threaded plate; 196, second filter layer; 197, connecting frame; 198, sealing plug; 20, connecting pipe; 21, feeding pump; 22, extraction bin; 23, water filtering mechanism; 231, collecting tank; 232, wire mesh frame; 233, counterweight shell; 234, airbag; 235, third filter layer; 236, baffle; 237, extraction pump; 238, washing liquid cover; 239, drain pipe; 24, storage box; 25, inclined plate; 26, counterweight block; 28, swing frame; 30, telescopic rod; 251, extrusion plate; 261, medicine injection pipe; 111, liquid seepage port; 222, guide rod. Detailed implementation manners

[0019] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0020] Please refer to Figure 1-7, the present invention provides a technical solution: including a fixed base 1, a rotating base 2 is connected to the top end of the fixed base 1, a driving member 3 is connected to the bottom end of the inner wall of the fixed base 1, the driving member 3 includes a centrifugal motor and a deceleration braking mechanism, which are conventional existing technologies and thus are not described in detail in the specification. The output end of the driving member 3 penetrates through the fixed base 1 and is connected to the rotating base 2. A plurality of positioning suction cups 4 are connected to the bottom end of the inner wall of the fixed base 1. Two fixed side plates 5 are connected to the top end of the rotating base 2. Rotating shafts 6 are rotatably arranged on the sides of the fixed side plates 5 close to each other. A connecting frame 7 is connected to the ends of the rotating shafts 6 close to each other. A dehydration bin 8 is connected to the inner side of the connecting frame 7. Fixed frames 9 are connected to both sides of the connecting frame 7. A top plate 10 is connected to the top end of the fixed frame 9. A pushing cylinder 11 is connected to the top end of the top plate 10. The output end of the pushing cylinder 11 penetrates through the top plate 10 and is connected to an extrusion mixing mechanism 12. A servo motor 13 is connected to the top end of the top plate 10. A plurality of connecting cylinders 14 are connected to the surface of the dehydration bin 8. The output end of the connecting cylinder 14 is connected to a deproteinization bin 15. Electric heating layers 16 are connected to the outer surfaces of both the deproteinization bin 15 and the dehydration bin 8. The output end of the servo motor 13 is connected to a driving shaft 18. The bottom end of the driving shaft 18 penetrates through the extrusion mixing mechanism 12 and the dehydration bin 8 and is rotatably arranged at the bottom end of the inner wall of the deproteinization bin 15. Filter mechanisms 19 are threadedly connected inside both the dehydration bin 8 and the deproteinization bin 15. Threads are provided on the inner walls of the dehydration bin 8 and the deproteinization bin 15 corresponding to the filter mechanisms 19. Connecting pipes 20 are connected to both sides of the deproteinization bin 15. The other ends of the connecting pipes 20 are connected to an extraction bin 22; A water filtering mechanism 23 is connected inside the extraction bin 22. A liquid discharging unit is connected inside the water filtering mechanism 23. A storage box 24 is connected to the bottom end of the extraction bin 22. The ends of the rotating shafts 6 far from each other both penetrate through the corresponding fixed side plates 5 and are connected to swing frames 28. An inclined plate 25 is connected to the top end of the swing frame 28. A counterweight 26 is fixedly arranged at the top end of the inclined plate 25. A telescopic rod 30 is rotatably arranged on one side of the swing frame 28. The other end of the telescopic rod 30 is rotatably arranged on one side of the fixed side plate 5; Refer to Figure 2 As shown, rectangular bars are connected to both sides of the driving shaft 18 inside the dehydration bin 8 and the deproteinization bin 15. The rectangular bars are slidably connected to the filter mechanism 19 and the extrusion mixing mechanism 12. While the driving shaft 18 rotates, the filter mechanism 19 and the extrusion mixing mechanism 12 can be driven to rotate together by the rectangular bars on both sides, so as to drive the filter mechanism 19 below to move up and down through forward and reverse rotation.

[0021] Refer to Figure 2 the intermediate amplification part and Figure 7As shown, an annular limiting groove is provided at the top of the extrusion and mixing mechanism 12. The bottom end of the pushing cylinder 11 is connected with a T-shaped arc block, which is slidably connected with the annular limiting groove. While the driving shaft 18 drives the extrusion and mixing mechanism 12 to rotate, the connection between the extrusion and mixing mechanism 12 and the pushing cylinder 11 is maintained through the sliding between the annular limiting groove and the T-shaped arc block.

[0022] Refer to Figure 7 As shown, the extrusion and mixing mechanism 12 includes a movable cover plate 121 slidably arranged in the dehydration bin 8. A rotating frame 122 is rotatably arranged at the top end of the movable cover plate 121. A stirring plate 123 is connected to the bottom end of the rotating frame 122. A rotating cylinder 124 is connected to one side of the rotating frame 122. The stirring plate 123 is fitted with the movable cover plate 121. When injecting activated carbon powder and extraction liquid into the dehydration bin 8, the rotating cylinder 124 drives the rotating frame 122 to rotate, so that the stirring plate 123 rotates into the dehydration bin 8, and stirring is carried out along with the rotation of the driving shaft 18. When filtering the activated carbon powder, the filtering mechanism 19 rises, and the pushing cylinder 11 presses down the extrusion and mixing mechanism 12 after reset and combination, so as to accelerate the filtering effect and fully squeeze out the liquid in the activated carbon powder.

[0023] Refer to Figure 5 As shown, the filtering mechanism 19 includes a filtering frame 191. A first filter layer 192 is connected to the top end of the filtering frame 191. A sealing shell 193 is connected to the bottom end of the filtering frame 191. An electric push rod 194 is connected inside the sealing shell 193. The output end of the electric push rod 194 penetrates through the sealing shell 193 and is connected with an arc-shaped threaded plate 195. A second filter layer 196 is connected to the bottom end of the filtering frame 191. The inner circle of the filtering frame 191 is slidably connected with the driving shaft 18. When filtering is required, the electric push rod 194 extends to push the arc-shaped threaded plate 195, so that it is threadedly connected with the inner wall of the corresponding decolorization bin 8 or 15. The filtering frame 191 drives the arc-shaped threaded plate 195 to rotate. With the guidance of the threaded connection, the whole filtering mechanism 19 starts to rise, and the activated carbon powder after adsorbing pigments is filtered out.

[0024] Refer to Figure 2 and Figure 5As shown in the figure, the bottom end of the filter rack 191 located in the dehydration bin 8 is connected with an adapter rack 197. A sealing plug 198 is rotatably arranged at the bottom end of the adapter rack 197. The bottom end of the dehydration bin 8 corresponding to the sealing plug 198 is set as a conical protruding end. The top end of the deproteinization bin 15 is provided with a conical concave end corresponding to the conical protruding end. And a pressing plate 251 is connected and arranged at the bottom end of the concave end. A medicine injection pipe 261 is connected and arranged at the top end of the pressing plate 251. When the connecting cylinder 14 contracts, it can drive the deproteinization bin 15 to move upward, and dock the conical protruding end at the bottom end of the dehydration bin 8 and the conical concave end at the top end of the deproteinization bin 15. While the dehydration bin 8 is being processed, the sealing plug 198 will block the conical protruding end at the bottom end of the dehydration bin 8. After the decolorization is completed, when the filter rack 191 rises, it drives the sealing plug 198 to rise through the adapter rack 197, so that the decolorized extract flows into the deproteinization bin 15.

[0025] Referring to Figure 3 and Figure 4 As shown in the figure, the water filtering mechanism 23 includes a collecting tank 231. A wire mesh rack 232 is connected and arranged on the outer side of the collecting tank 231. A counterweight shell 233 is connected and arranged on the outer side of the wire mesh rack 232. One side of the inner wall of the extraction bin 22 is connected and arranged with a guide rod 222. A fitting groove corresponding to the guide rod 222 is opened on the outer ring of the counterweight shell 233. An air bag 234 is connected and arranged inside the counterweight shell 233. A third filter layer 235 is connected and arranged at the bottom end of the wire mesh rack 232. A liquid discharging unit is connected and arranged in the collecting tank 231. A plurality of liquid seepage openings 111 are opened on the outer surface of the collecting tank 231. After the processed extract is injected into the extraction bin 22, the overall weight of the counterweight shell 233 and the mechanism will cause the entire water filtering mechanism 23 to sink along the guide rod 222. During the sinking process, the liquid will pass through the third filter layer 235 and the wire mesh rack 232, and enter the collecting tank 231 through the liquid seepage openings 111. It can be seen from the attached drawings that the central position of the collecting tank 231 is recessed, which can achieve a good water collection effect and facilitate the suction of the liquid discharging unit. At the same time, the air bag 234 can prevent the entire mechanism from sinking too fast and affecting the filtering effect. Among them, the first filter layer 192, the second filter layer 196 and the third filter layer 235 can select specific filtering mesh numbers according to needs; The liquid discharging unit includes a baffle 236 fixedly arranged inside the collecting tank 231. A pumping pump 237 is connected and arranged at the top end of the baffle 236. The water inlet end of the pumping pump 237 extends below the baffle 236 and is connected and arranged with a washing liquid cover 238. The water outlet end of the pumping pump 237 is connected and arranged with a liquid discharging pipe 239. The filtered liquid collected in the collecting tank 231 is pumped out through the washing liquid cover 238 by the small pumping pump 237 and discharged through the liquid discharging pipe 239. The other end of the liquid discharging pipe 239 is connected to an external collecting device. Since it is a conventional setting, it is not described in detail in the specification.

[0026] A method for purifying and separating a Hericium erinaceus polysaccharide extraction device includes the following steps: (1) Decolorization treatment: Inject activated carbon powder and the preliminarily extracted polysaccharide extract of Hericium erinaceus into the dehydration bin 8. Then, extend the pushing cylinder 11 to push the extrusion and mixing mechanism 12 into the top of the dehydration bin 8, thereby sealing the dehydration bin 8. Then, rotate the cylinder 124 to drive the rotating frame 122 to rotate, so that the stirring plate 123 rotates and extends into the dehydration bin 8. Start the servo motor 13, drive the movable cover plate 121 to rotate through the drive shaft 18, and use the stirring plate 123 to stir the extract. At the same time, the electric heating layer 16 outside the dehydration bin 8 heats the extract in the dehydration bin 8 to improve the mixing effect. After mixing, the pushing cylinder 11 lifts the extrusion and mixing mechanism 12, and then retracts the stirring plate 123. At the same time, the electric push rod 194 in the filtering mechanism 19 in the dehydration bin 8 extends, pushing the arc-shaped threaded plate 195 to be threadedly connected to the inner wall of the dehydration bin 8. Then, as the filter rack 191 rotates, the whole filtering mechanism 19 is driven to rise, and the activated carbon powder adsorbed with pigments in the extract is filtered through the first filter layer 192, the filter rack 191, and the second filter layer 196; (2) Protein removal treatment: The decolorized extract is discharged into the protein removal bin 15, and then the corresponding protease is injected into the protein removal bin 15 through the medicine injection pipe 261, and is mixed with the decolorized extract during the injection process. At this time, the telescopic rod 30 can be started to extend and push the swing frame 28 to rotate, so as to drive the connecting frame 7 to rotate through the rotating shaft 6, thereby tilting the dehydration bin 8 and the protein removal bin 15. Then, start the driving member 3 to drive the rotating base 2 to rotate, and perform centrifugation on the mixed solution in the protein removal bin 15. After centrifugation, the filtering mechanism 19 in the protein removal bin 15 is also started and rotated and lifted, filtering out the protein precipitate in the solution. Then, the feeding pump 21 pumps out the filtered solution. During the pumping process, negative pressure can also be created in the protein removal bin 15 to improve the filtering effect, and the solution is discharged into the extraction bin 22; (3) Polysaccharide extraction: After injecting the corresponding purified polysaccharide solution into the extraction bin 22, inject ethanol or methanol to precipitate the polysaccharide inside. Then, put the water filtering mechanism 23 into the extraction bin 22. As the water filtering mechanism 23 sinks, the third filter layer 235 filters out the liquid. The liquid passes through the wire mesh frame 232 and then enters the collection tank 231 through the liquid seepage port 111. The airbag 234 can prevent the water filtering mechanism 23 from sinking too fast. After the liquid enters the collection tank 231, start the extraction pump 237, pump out the liquid through the washing liquid cover 238, and discharge it through the drain pipe 239 until the liquid is finally squeezed out. Finally, remove the storage box 24, which is the purified polysaccharide.

[0027] The implementation principle of this application is as follows: When in use, this invention has the advantages of simple structure, convenient use, and good use effect.

[0028] It should be noted that, in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, such that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.

[0029] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A purification and separation device for extracting polysaccharides from Hericium erinaceus, comprising a fixed base (1), characterized in that: The top of the fixed base (1) is connected to a rotating base (2), the bottom of the inner wall of the fixed base (1) is connected to a driving member (3), the output end of the driving member (3) passes through the fixed base (1) and is connected to the rotating base (2), the bottom of the inner wall of the fixed base (1) is connected to a plurality of positioning suction cups (4), the top of the rotating base (2) is connected to two fixed side plates (5), the sides of the fixed side plates (5) close to each other are both rotatably provided with rotating shafts (6), the ends of the rotating shafts (6) close to each other are connected to a connecting frame (7), the inner side of the connecting frame (7) is connected to a dehydration bin (8), the two sides of the connecting frame (7) are connected to fixed frames (9), the top of the fixed frame (9) is connected to a top plate (10), the top of the top plate (10) is connected to a pushing cylinder (11), the output end of the pushing cylinder (11) passes through the top plate (10) and is connected to an extrusion mixing cylinder (11) The mechanism (12) comprises a servo motor (13) connected to the top of the top plate (10), a plurality of connecting cylinders (14) connected to the surface of the dehydration bin (8), a deproteinization bin (15) connected to the output end of the connecting cylinder (14), an electric heating layer (16) connected to the outer surface of the deproteinization bin (15) and the dehydration bin (8), a driving shaft (18) connected to the output end of the servo motor (13), the bottom end of the driving shaft (18) passing through the extrusion mixing mechanism (12) and the dehydration bin (8), and being rotatably arranged with the bottom end of the inner wall of the deproteinization bin (15), a filtering mechanism (19) connected to the inner wall of the dehydration bin (8) and the deproteinization bin (15) being threadedly connected, the inner walls of the dehydration bin (8) and the deproteinization bin (15) being threadedly arranged corresponding to the filtering mechanism (19), connecting pipes (20) connected to both sides of the deproteinization bin (15), and an extraction bin (22) connected to the other end of the connecting pipe (20); The extraction chamber (22) is internally connected with a water filter mechanism (23), and the water filter mechanism (23) is internally connected with a liquid discharge unit. The bottom end of the extraction chamber (22) is connected with a storage box (24). The ends of the rotating shafts (6) that are away from each other penetrate the corresponding fixed side plates (5) and are connected with a swing frame (28). The top end of the swing frame (28) is connected with an inclined plate (25), and the top end of the inclined plate (25) is fixedly provided with a counterweight (26). A telescopic rod (30) is rotatably provided on one side of the swing frame (28), and the other end of the telescopic rod (30) is rotatably provided on one side of the fixed side plate (5).

2. The purification and separation device for extracting Hericium erinaceus polysaccharides according to claim 1, characterized in that: The driving shaft (18) is provided with rectangular bars on both sides of the dehydration bin (8) and the deproteinization bin (15), and is slidably connected to the filtering mechanism (19) and the extrusion mixing mechanism (12) via the rectangular bars.

3. The purification and separation device for extracting Hericium erinaceus polysaccharides according to claim 2, characterized in that: The top end of the extrusion mixing mechanism (12) is provided with an annular limiting groove, and the bottom end of the pushing cylinder (11) is connected with a T-shaped arc block, which is slidably connected to the annular limiting groove.

4. The purification and separation device for extracting Hericium erinaceus polysaccharide according to claim 3, characterized in that: The extrusion mixing mechanism (12) comprises a movable cover plate (121) slidably arranged in the dehydration bin (8); a rotating frame (122) is rotatably arranged at the top end of the movable cover plate (121); a stirring plate (123) is connected to the bottom end of the rotating frame (122); a rotating cylinder (124) is connected to one side of the rotating frame (122); and the stirring plate (123) is engaged with the movable cover plate (121).

5. The purification and separation device for extracting Hericium erinaceus polysaccharides according to claim 4, characterized in that: The filtering mechanism (19) comprises a filtering frame (191), the top end of the filtering frame (191) being connected to a first filtering layer (192), the bottom end of the filtering frame (191) being connected to a sealing shell (193), the sealing shell (193) being internally connected to an electric push rod (194), the output end of the electric push rod (194) passing through the sealing shell (193) and being connected to an arc-shaped threaded plate (195), and the bottom end of the filtering frame (191) being connected to a second filtering layer (196).

6. The purification and separation device for extracting Hericium erinaceus polysaccharide according to claim 5, characterized in that: The bottom end of the filter frame (191) located in the dehydration bin (8) is connected to a connecting frame (197), and a sealing plug (198) is rotatably provided at the bottom end of the connecting frame (197). The bottom end of the dehydration bin (8) is provided with a conical protruding end corresponding to the sealing plug (198), and the top end of the deproteinization bin (15) is provided with a conical concave end corresponding to the conical protruding end, and the bottom end of the concave end is connected to an extrusion plate (251), and the top end of the extrusion plate (251) is connected to a drug injection tube (261).

7. The purification and separation device for extracting Hericium erinaceus polysaccharide according to claim 6, characterized in that: The water filtering mechanism (23) comprises a collecting trough (231), a grid frame (232) is connected to the outside of the collecting trough (231), a counterweight shell (233) is connected to the outside of the grid frame (232), an air bag (234) is connected to the inside of the counterweight shell (233), a third filter layer (235) is connected to the bottom end of the grid frame (232), the liquid drainage unit is connected to the inside of the collecting trough (231), and a plurality of liquid seepage ports (111) are provided on the outer surface of the collecting trough (231).

8. The purification and separation device for extracting Hericium erinaceus polysaccharide according to claim 7, characterized in that: The liquid discharge unit comprises a baffle (236) fixedly arranged on the inner side of the collection tank (231); a pumping pump (237) is connected to the top end of the baffle (236); a water inlet end of the pumping pump (237) extends below the baffle (236) and is connected to a washing liquid cover (238); and a liquid discharge pipe (239) is connected to the water outlet end of the pumping pump (237).

9. The purification and separation device for extracting Hericium erinaceus polysaccharide according to claim 8, characterized in that: A guide rod (222) is connected to one side of the inner wall of the extraction bin (22), and an outer ring of the counterweight shell (233) is provided with an engaging groove corresponding to the guide rod (222).

10. The method for extracting and purifying Hericium erinaceus polysaccharide according to claim 9, comprising the following steps: (1) Decolorization treatment: activated carbon powder and polysaccharide extract initially extracted from Hericium erinaceus are injected into the dehydration chamber (8), and then the cylinder (11) is pushed to extend and push the extrusion mixing mechanism (12) into the top of the dehydration chamber (8), thereby sealing the dehydration chamber (8), and then the rotating cylinder (124) drives the rotating frame (122) to rotate, and the stirring plate (123) rotates and extends into the dehydration chamber (8), and the servo motor (13) is started to drive the movable cover plate (121) to rotate through the driving shaft (18), and the extract is stirred by the stirring plate (123), and at the same time, the electric heating layer (16) outside the dehydration chamber (8) heats the dehydration chamber. The extract in the dehydration bin (8) is heated to improve the mixing effect. After the mixing is completed, the cylinder (11) is pushed to lift the extrusion mixing mechanism (12), and then the stirring plate (123) is retracted. At the same time, the electric push rod (194) in the filter mechanism (19) in the dehydration bin (8) is extended to push the arc-shaped threaded plate (195) to connect with the thread of the inner wall of the dehydration bin (8). Then, as the filter frame (191) rotates, the filter mechanism (19) is driven to rise as a whole, and the activated carbon powder adsorbed with the pigment in the extract is filtered through the first filter layer (192), the filter frame (191) and the second filter layer (196); (2) Deproteinization: the decolorized extract is discharged into the deproteinization chamber (15), and then the corresponding protease is injected into the deproteinization chamber (15) through the injection tube (261). During the injection process, the protease is mixed with the decolorized extract. At this time, the telescopic rod (30) can be started to extend and push the swing frame (28) to rotate, thereby driving the connecting frame (7) to rotate through the rotating shaft (6), so that the dehydration chamber (8) and the deproteinization chamber (15) are tilted, and then the driving member (3) is started to drive the rotating base (2) to rotate, and the mixed solution in the deproteinization chamber (15) is centrifuged. After the centrifugation is completed, the filtering mechanism (19) in the deproteinization chamber (15) is also started and rises with the rotation to filter out the protein precipitate in the solution. Then the feeding pump (21) extracts the filtered solution. During the extraction process, negative pressure can also be created in the deproteinization chamber (15) to improve the filtering effect, and the solution is discharged into the extraction chamber (22); (3) Polysaccharide extraction: After the corresponding purified polysaccharide solution is injected into the extraction chamber (22), ethanol or methanol is injected to precipitate the polysaccharide inside, and then the water filter mechanism (23) is placed in the extraction chamber (22). As the water filter mechanism (23) sinks, the third filter layer (235) filters out the liquid, and the liquid passes through the mesh frame (232) and then enters the collection tank (231) through the seepage port (111). The air bag (234) can prevent the water filter mechanism (23) from sinking too quickly. After the liquid enters the collection tank (231), the extraction pump (237) is turned on to extract the liquid through the washing liquid cover (238) and discharge it through the drainage pipe (239) until the liquid is finally squeezed out. Finally, the storage box (24) is removed, and the purified polysaccharide is obtained.

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