A tumor cell cluster sorting and filtering device for oncology

Abstract

The present invention discloses a tumor cell cluster separation and filtration device for oncology, belonging to the field of tumor cell cluster processing technology. It comprises a bottom tube seat, a flow storage column, a filtration unit, a scrubbing unit, an interception and filtration unit, an air pressure unit and a collection tank. The two ends of the bottom tube seat are hollow, the middle part of the bottom tube seat is integrally formed with a partition, and the bottom tube seat is provided with a grille slot above the partition; the middle part of the partition is provided with a pipe through hole and an exhaust hole; the bottom of the flow storage column is screwed to the top surface of the bottom tube seat; the bottom of the flow storage column is provided with a convex flow guide hole; the top of the flow storage column is fixed with a top cover; the middle part of the top cover is provided with a liquid inlet valve; the inner wall of the flow storage column is provided with multiple flow channels; the tumor cell cluster separation and filtration device for oncology of the present invention can fully extract tumor cell clusters from the dispersion liquid, ensure the dispersion and filtration efficiency of the tumor cell clusters, and protect the extracted tumor cell clusters.

Description

Technical Field

[0001] The present invention specifically relates to a tumor cell cluster sorting and filtering device for oncology, and belongs to the technical field of tumor cell cluster processing. Background Art

[0002] During their work, doctors in the oncology department often need to sort and filter tumor cell clusters through a sorting and filtering device, that is, adding fresh tissue dispersion into the sorting and filtering device to sort and filter the tumor cell clusters. Since the size of larger tissues such as fat tissue, membrane tissue, connective tissue in the tissue dispersion is hundreds of microns, while the size of tumor cell clusters in the tissue dispersion is generally tens of microns, the larger tissues often clog the filter plate in the sorting and filtering device, affecting the sorting of tumor cell clusters. Most existing tumor cell cluster sorting and filtering devices use vibration to avoid clogging of the filter plate. However, the water flow generated by the vibration can only disperse the deposits accumulated on the surface of the filter plate, and cannot easily wash away the debris stuck in the upper port of the filter hole, especially It is impossible to flush away the debris stuck in the filter holes; for this reason, Chinese patent authorization announcement number: CN114797215B discloses a tumor cell cluster sorting and filtering device and method, which can form a negative pressure in the area below the filter plate in the filter chamber, and can form a positive pressure in the area above the filter plate in the filter chamber, thereby accelerating the filtration speed of tumor cell clusters in the mixed liquid above the filter plate. However, the existing sorting and filtering device cannot fully sort out tumor cell clusters from the tissue dispersion liquid, and tumor cell clusters are easily trapped between fat tissue, membrane tissue and connective tissue; in addition, when it is necessary to fully extract the tumor cell clusters, the already extracted tumor cell clusters will be continuously impacted, resulting in damage to the tumor cell clusters, and the sorting and filtration time will also be increased. Summary of the Invention

[0003] To solve the above problems, the present invention proposes a tumor cell cluster sorting and filtration device for oncology, which can fully extract tumor cell clusters from the dispersion liquid, ensure the dispersion and filtration efficiency of tumor cell clusters, and protect the extracted tumor cell clusters.

[0004] The tumor cell cluster separation and filtration device for oncology of the present invention comprises:

[0005] The bottom tube seat is hollow at both ends, and a partition is integrally formed in the middle of the bottom tube seat. A grille slot is provided above the partition; a tube hole and an exhaust hole are provided in the middle of the partition;

[0006] A flow storage column; the bottom of the flow storage column is screwed to the top surface of the bottom tube seat; a convex flow guide hole is provided at the bottom of the flow storage column; a top cover is fixed to the top of the flow storage column; a liquid inlet valve is provided in the middle of the top cover; a plurality of flow channels are opened on the inner wall of the flow storage column;

[0007] The filter unit includes an upper filter and a lower filter of the same model that are interconnected; the bottoms of the upper filter and the lower filter are connected to an air pressure valve; the bottoms of the upper filter and the lower filter are provided with a liquid discharge port; the liquid inlet end of the upper filter is connected to the liquid inlet valve; the air pressure valve of the lower filter is connected to a collection pipe; the collection pipe is sealed and passes through the fluid storage column and is movable through the pipe hole;

[0008] The scrubbing unit includes a venturi ejector that moves through the grille slot; the liquid inlet end of the venturi ejector is sealed and engaged with the guide hole; the output end of the venturi ejector is connected to a standpipe, the upper end of the standpipe is connected to the slow flow bucket; the bottom of the slow flow bucket is connected to a plug connector;

[0009] The intercepting and filtering unit has a bottom portion connected to the liquid inlet valve, and the plug connector is movably connected to the top portion of the intercepting and filtering unit;

[0010] An air pressure unit, the air pressure unit is connected to the input end of the venturi ejector and the air pressure valve respectively;

[0011] The collecting tank is movably embedded in the inner side of the bottom pipe seat and movably plugged into the collecting pipe.

[0012] During operation, fresh tissue dispersion is sent to the interception and filtration unit, and larger tissues such as fat tissue, membrane tissue, connective tissue, etc. in the tissue dispersion are intercepted by the interception and filtration unit; then, the dispersion and tumor cell clusters are injected into the filtration unit. At this time, the air pressure valve at the bottom of the lower filter is locked, and the dispersion and tumor cell clusters are collected through the lower filter. When the scrubbing unit reaches the action time, the air pressure valve at the bottom of the upper filter is locked; at this time, the air pressure unit provides a pressure air source to the Venturi ejector, and the dispersion in the flow storage column enters the Venturi ejector through the guide hole, and the dispersion is formed into a jet through the air source and sent to the riser, and is buffered by the slow flow bucket; after the flow is stored through the slow flow bucket, it is sent back to the interception and filtration unit. , and flush and clean the larger tissues such as fat tissue, membrane tissue, connective tissue, etc. retained by the retention filter unit; then, the air pressure valve at the bottom of the lower filter is opened, and the tumor cell clusters and the remaining dispersion are sent to the collection tank for collection, and the remaining dispersion is discharged to prevent the enriched tumor cell clusters from being flushed; then, before the next venturi ejector is activated, the air pressure valve at the bottom of the upper filter is opened, and the tumor cell clusters and dispersion flow into the lower filter to flush the filter membrane of the lower filter. At the same time, the dispersion is filtered out again by the lower filter; after the upper filter is emptied, the air pressure valve at the bottom of the upper filter is locked again, and the venturi ejector is activated, and this is performed multiple times in sequence to re-collect the tumor cell clusters.

[0013] Furthermore, the flow storage column is radially sealed and plugged with an air pressure tube; one end of the air pressure tube is screwed and fixed to the air pressure valve, and the other end is connected to the air pressure unit; the air pressure unit provides a pressure air source to the air pressure tube, and the pressure air source is injected into the air pressure valve to control the action of the air pressure valve, and the air pressure tube can support the filter unit.

[0014] Furthermore, the air pressure unit includes a diverter block, a transverse flow channel is opened on the inner side of the diverter block, and three longitudinal flow channels are passed through the transverse flow channel; one end of the transverse flow channel is connected to the pressure air source through a pressure regulating valve, and the other end is connected to the venturi ejector through a first switch valve and a hose; an emptying valve is screwed on one of the longitudinal flow channels, and a second switch valve is screwed on the other two longitudinal flow channels; the second switch valve is connected to the air pressure valve through a transition hose; when passing through, the clean air source enters the transverse flow channel through the pressure regulating valve, and adjusts the pressure regulating valve to respond to the pressure according to the pressure requirement, and the air source with completed pressure adjustment is sent to the first switch valve or the second switch valve; thereby providing a pressurized air flow to the venturi ejector through the first switch valve, so that the dispersion is formed into a jet and injected into the slow flow bucket; or providing pressure to the air pressure valve through the second switch valve, so that the second switch valve is locked; when the second switch valve needs to be opened again, the pressure regulating valve is turned off, and the emptying valve is opened to discharge the air source inside the pressure regulating valve.

[0015] Furthermore, the bottom tube seat is movably connected to the plug interface of the oscillator; through the oscillating force of the oscillator and the backflow flushing of the jet, the tumor cell clusters can be fully sorted and filtered, and the filtration efficiency can be improved.

[0016] Furthermore, a plurality of Y-shaped support blocks are movably inserted into the top surface of the partition. After the bottom of the support block abuts against the top surface of the collecting tank, the upper part of the support block is engaged with the Venturi ejector. When in use, the Venturi ejector is first embedded into the grille groove, and the Venturi ejector is aligned with the guide hole. Then, the collecting tank is embedded into the inner side of the bottom pipe seat, and the support block is pushed up through the collecting tank to achieve the engagement and limiting of the support block and the Venturi ejector.

[0017] Furthermore, the collection tank includes an outer cup body, a collection filter tank is embedded in the top surface of the outer cup body, and the top surface of the collection filter tank is provided with an air leakage hole and a through hole connected to the collection pipe; a flow storage gap is provided between the collection filter tank and the outer cup body; the interception filter unit includes a first inner filter tank, and a first collecting tank is provided on the outside of the first inner filter tank; a first docking plug is provided on the first collecting tank; the first docking plug is docked with the first inner filter tank; the upper filter and the lower filter include a second inner filter tank, and a second collecting tank is provided on the outside of the second inner filter tank; a drain port is provided at the bottom of the second collecting tank; the bottom of the second collecting tank is connected to the air pressure valve; a second docking plug is provided on the top of the second collecting tank; the first collecting tank and the second collecting tank include a tank body, and a cover body is screwed on the top of the tank body.

[0018] The interception and filtration unit receives fresh tissue dispersion, and the fresh tissue dispersion enters the interception and filtration unit, and the fat tissue, membrane tissue, and connective tissue are intercepted by the filter membrane of the first inner filter tank, so that the dispersion and tumor cell clusters enter the first collecting tank; the dispersion and tumor cell clusters are sent to the second inner filter tank through the first collecting tank, and the dispersion is separated into the second collecting tank through the second inner filter tank, and the dispersion is sent to the flow channel through the discharge port of the second collecting tank, so that the dispersion flows to the bottom of the inner side of the flow storage column; after the treatment is completed, the tumor cell clusters and residual dispersion in the second inner filter tank are sent to the collection filter tank, and the residual dispersion is discharged into the flow storage gap through the collection filter tank; the tumor cell clusters after sorting and filtration can be enriched by the collection filter tank.

[0019] Furthermore, the collection filter tank, the first inner filter tank and the second inner filter tank all include an outer filter cartridge and an inner filter cartridge, and a filter membrane is sandwiched between the outer filter cartridge and the inner filter cartridge; the pore size of the filter membrane of the collection filter tank and the second inner filter tank is 8~15μm; the pore size of the filter membrane of the first inner filter tank is 50~80μm; during operation, when the dispersion is sent into the first inner filter tank, the fat tissue, membrane tissue, and connective tissue are intercepted by the filter membrane of the first inner filter tank, so that the dispersion and tumor cell clusters enter the second inner filter tank; the dispersion is discharged to the outside of the second inner filter tank through the second inner filter tank, and finally the collected tumor cell clusters are sent to the collection filter tank for collection, and then the dispersion is discharged from the collection filter tank and collected in the flow storage gap; finally, the collection filter tank can be directly taken out to obtain the enriched tumor cell clusters.

[0020] The cam is pressed downwards to release the cam, and the cam is in a state of being in contact with the valve core, and the cam is in a state of being in contact with the valve core, and the cam is in a state of being in contact with the valve core.

[0021] Furthermore, a plurality of deflectors are provided inside the slow-flow bucket; the plug connector includes a hose section connected to the bottom of the slow-flow bucket, and a plug is connected to the bottom of the hose section; a second spring body is provided between the hose section and the plug and the slow-flow bucket; the flow velocity of the jet can be reduced by the large volume of the slow-flow bucket and the obstruction of the deflector; when the plug connector is plugged into the interception and filtration unit, the plug is plugged into the top of the interception and filtration unit, and when the plug connector needs to be unplugged, the plug is lifted and the second spring body is compressed, thereby separating the plug connector from the interception and filtration unit.

[0022] Furthermore, a liquid level sensing unit is provided at the lower part of the Venturi ejector. The liquid level sensing unit adopts two electrode heads. When the fluid is accumulated in the Venturi ejector, the two electrode heads are short-circuited, and the liquid level sensing unit can detect the liquid level; the liquid level sensing unit triggers the air pressure unit through the controller controlled by the whole machine; the Venturi ejector is actuated, and the filtrate is formed into a jet and is sent back to the interception filter unit; the liquid level sensing unit is used to prevent the air pressure unit from being in a continuous working state.

[0023] Compared with the prior art, the tumor cell cluster sorting and filtration device for oncology of the present invention adopts the jet provided by the brushing unit to fully flush the fat tissue, membrane tissue and connective tissue in the dispersion liquid, realize multiple brushing of the dispersion liquid components, and can fully extract tumor cell clusters from the dispersion liquid. Through the cooperation of the upper filter and the lower filter, the tumor cell clusters obtained after the dispersion filtration can be collected separately to avoid secondary flushing by the filtrate, protect the tumor cell clusters, and avoid the enrichment of tumor cell clusters and affecting the dispersion and filtration efficiency of the filter membrane. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 Schematic diagram of the overall structure of the tumor cell cluster separation and filtration device of the present invention.

[0025] Figure 2 For the present invention Figure 1 Schematic diagram of the locally enlarged structure at point A in the middle.

[0026] Figure 3 It is a schematic diagram of the internal structure of the bottom pipe seat and the flow storage pipe column in the axial section of the present invention.

[0027] Figure 4 It is a schematic diagram of the overall structure of the collection tank of the present invention.

[0028] Figure 5 It is a schematic diagram of the overall structure of the interception and filtration unit of the present invention.

[0029] Figure 6 It is a schematic diagram of the overall structure of the upper filter of the present invention.

[0030] Figure 7 It is a schematic diagram of the cross-sectional structure of the air pressure valve of the present invention.

[0031] Figure 8 This is a schematic diagram of the installation structure of the Venturi ejector and liquid level sensing unit of the present invention.

[0032] Reference numerals: 1, bottom pipe seat, 2, partition, 3, grid slot, 4, exhaust hole, 5, flow storage column, 6, diversion hole, 7, top cover, 8, liquid inlet valve, 9, upper filter, 10, lower filter, 11, air pressure valve, 12, drain port, 13, collecting pipe, 14, Venturi ejector, 15, standpipe, 16, slow flow bucket, 17, plug connector, 18, interception filter unit, 19, collecting tank, 20, air pressure pipe, 21, diverter block, 22, pressure regulating valve, 23, first switch valve, 24. Drain valve, 25. Second switch valve, 26. Support block, 27. Outer cup, 28. Collection filter tank, 29. Air vent, 30. First inner filter tank, 31. First collecting tank, 32. First docking plug, 33. Second inner filter tank, 34. Second collecting tank, 35. Second docking plug, 36. Valve tube, 37. Valve core cavity, 38. Valve core, 39. First spring body, 40. Fixed core column, 41. Hose section, 42. Plug, 43. Second spring body, 44. Liquid level sensing unit. DETAILED DESCRIPTION

[0033] Example:

[0034] like Figures 1 to 8 The tumor cell cluster separation and filtration device shown in the oncology department comprises:

[0035] A bottom tube base 1, wherein both ends of the bottom tube base 1 are hollow, a partition 2 is integrally formed in the middle of the bottom tube base 1, and a grille slot 3 is provided above the partition 2; a tube hole and an exhaust hole 4 are provided in the middle of the partition 2;

[0036] The fluid storage column 5; the bottom of the fluid storage column 5 is screwed to the top surface of the bottom tube seat 1; the bottom of the fluid storage column 5 is provided with a convex guide hole 6; the top of the fluid storage column 5 is fixed with a top cover 7; the middle of the top cover 7 is provided with a liquid inlet valve 8; the inner wall of the fluid storage column 5 is provided with multiple flow channels;

[0037] The filter unit includes an upper filter 9 and a lower filter 10 that are interconnected and of the same model. The bottoms of the upper filter 9 and the lower filter 10 are connected to an air pressure valve 11. The bottoms of the upper filter 9 and the lower filter 10 are provided with a liquid discharge port 12. The liquid inlet end of the upper filter 9 is connected to the liquid inlet valve 8. The air pressure valve 11 of the lower filter 10 is connected to a collection pipe 13. The collection pipe 13 is sealed and passes through the fluid storage column 5 and is movable through the pipe hole.

[0038] The scrubbing unit includes a venturi ejector 14 that moves through the grille slot 3; the liquid inlet end of the venturi ejector 14 is sealed and engaged with the guide hole 6; the output end of the venturi ejector 14 is connected to a standpipe 15, the upper end of the standpipe 15 is connected to a slow flow bucket 16; the bottom of the slow flow bucket 16 is connected to a plug connector 17;

[0039] The retaining filter unit 18 has a bottom connected to the liquid inlet valve 8, and the plug connector 17 is movably connected to the top of the retaining filter unit 18;

[0040] An air pressure unit, the air pressure unit is connected to the input end of the venturi ejector 14 and the air pressure valve 11 respectively;

[0041] The collecting tank 19 is movably embedded in the inner side of the bottom tube seat 1 and movably plugged into the collecting tube 13 .

[0042] During operation, the fresh tissue dispersion is sent to the interception and filtration unit 18, and the larger tissues such as fat tissue, membrane tissue, connective tissue, etc. in the tissue dispersion are intercepted by the interception and filtration unit 18; then, the dispersion and tumor cell clusters are injected into the filtration unit. At this time, the air pressure valve 11 at the bottom of the lower filter 10 is locked, and the dispersion and tumor cell clusters are collected by the lower filter 10. When the scrubbing unit reaches the action time, the air pressure valve 11 at the bottom of the upper filter 9 is locked; at this time, the air pressure unit provides a pressure air source to the Venturi ejector 14, and the dispersion in the flow storage column 5 enters the Venturi ejector 14 through the guide hole 6, and is formed into a jet by the air source and sent to the standpipe 15, and is buffered by the slow flow bucket 16; after the flow is stored by the slow flow bucket 16, it is sent back to the interception and filtration unit 1 8, and flush and clean the larger tissues such as fat tissue, membrane tissue, connective tissue, etc. retained by the retention filter unit 18; then, the air pressure valve 11 at the bottom of the lower filter 10 is opened, and the tumor cell clusters and the remaining dispersion liquid are sent to the collection tank 19 for collection, and the remaining dispersion liquid is discharged to prevent the enriched tumor cell clusters from being flushed; then, before the next venturi ejector 14 is activated, the air pressure valve 11 at the bottom of the upper filter 9 is opened, and the tumor cell clusters and the dispersion liquid flow into the lower filter 10 to flush the filter membrane of the lower filter 10. At the same time, the dispersion liquid is filtered out again by the lower filter 10; after the upper filter 9 is emptied, the air pressure valve 11 at the bottom of the upper filter 9 is locked again, and the venturi ejector 14 is activated, and this is performed multiple times in sequence to re-collect the tumor cell clusters.

[0043] The flow storage column 5 is radially sealed and plugged with an air pressure tube 20; one end of the air pressure tube 20 is screwed and fixed to the air pressure valve 11, and the other end is connected to the air pressure unit; the air pressure unit provides a pressure air source to the air pressure tube 20, and the pressure air source is injected into the air pressure valve 11 to control the action of the air pressure valve 11, and the air pressure tube 20 can support the filter unit.

[0044] The air pressure unit includes a diverter block 21, a transverse flow channel is provided inside the diverter block 21, and three longitudinal flow channels are passed through the transverse flow channel; one end of the transverse flow channel is connected to the pressure gas source through a pressure regulating valve 22, and the other end is connected to the venturi ejector 14 through a first switch valve 23 and a hose; an emptying valve 24 is screwed on one of the longitudinal flow channels, and a second switch valve 25 is screwed on the other two longitudinal flow channels; the second switch valve 25 is connected to the air pressure valve 11 through a transition hose; when passing through, the clean air source enters the transverse flow channel through the pressure regulating valve 22 The pressure regulating valve 22 is adjusted to respond to the pressure according to the pressure requirement, and the gas source with pressure adjustment is sent to the first switch valve 23 or the second switch valve 25; thereby, the first switch valve 23 provides a pressurized airflow for the venturi ejector 14, so that the dispersion liquid is formed into a jet and injected into the slow flow bucket 16; or the second switch valve 25 provides pressure for the air pressure valve 11, so that the second switch valve 25 is locked; when the second switch valve 25 needs to be opened again, the pressure regulating valve 22 is closed, and the drain valve 24 is opened to discharge the gas source inside the pressure regulating valve 22.

[0045] The bottom tube seat 1 is movably connected to the plug interface of the oscillator; through the oscillating force of the oscillator and the backflow flushing of the jet, the tumor cell clusters can be fully sorted and filtered, and the filtration efficiency can be improved.

[0046] A plurality of Y-shaped support blocks 26 are movably inserted into the top surface of the partition 2. After the bottom of the support block 26 abuts against the top surface of the collecting tank 19, the upper part of the support block 26 is engaged with the venturi ejector 14. When in use, the venturi ejector 14 is first embedded in the grille slot 3, and the venturi ejector 14 is aligned with the guide hole 6. Then, the collecting tank 19 is embedded into the inner side of the bottom pipe seat 1, and the support block 26 is pushed up by the collecting tank 19 to achieve the engagement and limiting of the support block 26 and the venturi ejector 14.

[0047] The collecting tank 19 includes an outer cup body 27, a collecting filter tank 28 is embedded in the top surface of the outer cup body 27, and the top surface of the collecting filter tank 28 is provided with an air leakage hole 29 and a through hole for plugging with the collecting pipe 13; a flow storage gap is provided between the collecting filter tank 28 and the outer cup body 27; the intercepting filter unit 18 includes a first inner filter tank 30, a first collecting tank 31 is provided outside the first inner filter tank 30; a first docking plug 32 is provided on the first collecting tank 31; the first docking plug 32 is docked with the first inner filter tank 30; the upper filter 9 and the lower filter 10 include a second inner filter tank 33, a second collecting tank 34 is provided outside the second inner filter tank 33; a drain port 12 is provided at the bottom of the second collecting tank 34; the bottom of the second collecting tank 34 is connected to the air pressure valve 11; a second docking plug 35 is provided on the top of the second collecting tank 34; the first collecting tank 31 and the second collecting tank 34 include a tank body, and a cover body is screwed on the top of the tank body.

[0048] The interception and filtration unit 18 receives fresh tissue dispersion, and the fresh tissue dispersion enters the interception and filtration unit 18, and the fat tissue, membrane tissue, and connective tissue are intercepted by the filter membrane of the first inner filter tank 30, so that the dispersion and tumor cell clusters enter the first collecting tank 31; the dispersion and tumor cell clusters are sent to the second inner filter tank 33 through the first collecting tank 31, and the dispersion is separated into the second collecting tank 34 through the second inner filter tank 33, and the dispersion is sent to the flow channel through the discharge port 12 of the second collecting tank 34, so that the dispersion flows to the inner bottom of the flow storage column 5; after the treatment is completed, the tumor cell clusters in the second inner filter tank 33 and the residual dispersion are sent to the collection filter tank 28, and the residual dispersion is discharged into the flow storage gap through the collection filter tank 28; the tumor cell clusters after sorting and filtration can be enriched by the collection filter tank 28.

[0049] The collecting filter tank 28, the first inner filter tank 30 and the second inner filter tank 33 all include an outer filter cartridge and an inner filter cartridge, with a filter membrane sandwiched between the outer filter cartridge and the inner filter cartridge; the pore size of the filter membrane of the collecting filter tank 28 and the second inner filter tank 33 is 8-15 μm; the pore size of the filter membrane of the first inner filter tank 30 is 50-80 μm; during operation, when the dispersion is fed into the first inner filter tank 30, the adipose tissue, membrane tissue and connective tissue are intercepted by the filter membrane of the first inner filter tank 30, allowing the dispersion and tumor cell clusters to enter the second inner filter tank 33; the dispersion is discharged from the outside of the second inner filter tank 33 through the second inner filter tank 33, and finally the collected tumor cell clusters are fed into the collecting filter tank 28 for collection, and then the dispersion is discharged from the collecting filter tank 28 and collected in the flow storage gap; finally, the enriched tumor cell clusters can be obtained by directly removing the collecting filter tank 28.

[0050] The air pressure valve 11 includes a valve tube 36 with a three-way structure, a valve core cavity 37 is provided in the middle of the valve tube 36, and the upper and lower ends of the valve tube 36 are a liquid inlet and a liquid outlet; a valve core 38 is movably provided at the middle end of the valve tube 36, and the valve core 38 and the valve core cavity 37 are movably engaged, and a first spring body 39 is movably engaged inside the valve core 38, and a fixed core column 40 is fixed inside the valve core cavity 37, and the fixed core column 40 is embedded in the valve core cavity 37 and abuts against the first spring body 39; the middle end of the valve tube 36 is screwed There is an air pressure tube 20; the end of the air pressure tube 20 is in contact with the valve core 38; when the pneumatic valve is actuated, the air source is injected into the middle end of the valve tube 36 through the air pressure tube 20, and the air source pushes the valve core 38 into the valve core cavity 37, and the valve core 38 slides along the fixed core column 40, and the first spring body 39 is compressed to block the liquid inlet and liquid outlet of the valve tube 36; when the valve tube 36 needs to be unlocked, the air source in the air pressure tube 20 is discharged, and at this time, the valve core 38 is reset by the elastic force of the first spring body 39, so that the valve core 38 is separated from the valve core cavity 37.

[0051] A plurality of baffles are provided inside the slow-flow bucket 16; the plug connector 17 includes a hose section 41 connected to the bottom of the slow-flow bucket 16, and a plug 42 is connected to the bottom of the hose section 41; the hose section 41 is provided with a second spring body 43 between the plug 42 and the slow-flow bucket 16; the flow rate of the jet can be reduced by the large volume of the slow-flow bucket 16 and the obstruction of the baffle; when the plug connector 17 is plugged into the retention filter unit 18, the plug 42 is plugged into the top of the retention filter unit 18. When the plug connector 17 needs to be unplugged, the plug 42 is lifted up and the second spring body 43 is compressed, thereby separating the plug connector 17 from the retention filter unit 18.

[0052] A liquid level sensing unit 44 is provided at the lower part of the venturi ejector 14. The liquid level sensing unit 44 adopts two electrode heads. When the fluid is accumulated in the venturi ejector 14, the two electrode heads are short-circuited, and the liquid level sensing unit 44 can detect the liquid level. The liquid level sensing unit 44 triggers the air pressure unit through the controller of the whole machine control. The venturi ejector 14 is actuated, and the filtrate is formed into a jet and is sent back to the interception filter unit 18. The liquid level sensing unit 44 is used to prevent the air pressure unit from being in a continuous working state.

[0053] The above embodiments are only preferred implementations of the present invention. Therefore, any equivalent changes or modifications made according to the structures, features and principles described in the scope of application of the present invention are included in the scope of application of the present invention.

Claims

1. A tumor cell cluster separation and filtration device for oncology, characterized by: include: A bottom tube seat, wherein both ends of the bottom tube seat are hollow, a partition is integrally formed in the middle of the bottom tube seat, and a grille slot is provided above the partition; The middle part of the partition is provided with a pipe hole and an exhaust hole; A flow storage column; the bottom of the flow storage column is screwed to the top surface of the bottom tube seat; a convex flow guide hole is provided at the bottom of the flow storage column; a top cover is fixed to the top of the flow storage column; a liquid inlet valve is provided in the middle of the top cover; a plurality of flow channels are opened on the inner wall of the flow storage column; A filter unit is provided inside the fluid storage column; the filter unit comprises an upper filter and a lower filter of the same model that are interconnected; an air pressure valve is connected to the bottom of the upper filter and the lower filter; a liquid discharge port is provided at the bottom of the upper filter and the lower filter; the liquid inlet end of the upper filter is connected to the liquid inlet valve; the air pressure valve of the lower filter is connected to a collection pipe; the collection pipe is sealed and passes through the fluid storage column and is movable through the pipe hole; The scrubbing unit includes a venturi ejector that moves through the grille slot; the liquid inlet end of the venturi ejector is sealed and engaged with the guide hole; the output end of the venturi ejector is connected to a standpipe, the upper end of the standpipe is connected to the slow flow bucket; the bottom of the slow flow bucket is connected to a plug connector; The intercepting and filtering unit has a bottom portion connected to the liquid inlet valve, and the plug connector is movably connected to the top portion of the intercepting and filtering unit; An air pressure unit, the air pressure unit is connected to the input end of the venturi ejector and the air pressure valve respectively; The collecting tank is movably embedded in the inner side of the bottom pipe seat and movably plugged into the collecting pipe.

2. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The flow storage column is radially sealed and plugged with an air pressure tube; one end of the air pressure tube is screwed and fixed to the air pressure valve, and the other end is connected to the air pressure unit.

3. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The air pressure unit includes a diverter block, a transverse flow channel is opened on the inner side of the diverter block, and three longitudinal flow channels are passed through the transverse flow channel; one end of the transverse flow channel is connected to the pressure air source through a pressure regulating valve, and the other end is connected to the Venturi ejector through a first switch valve and a hose; an emptying valve is screwed on one of the longitudinal flow channels, and the other two longitudinal flow channels are screwed on the second switch valve; the second switch valve is connected to the air pressure valve through a transition hose.

4. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The bottom tube seat is movably plugged into the plug interface of the oscillator.

5. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: A plurality of Y-shaped support blocks are movably plugged into the top surface of the partition. After the bottom of the support block abuts against the top surface of the collection tank, the upper portion of the support block is clamped with the Venturi ejector.

6. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The collecting tank includes an outer cup body, a collecting filter tank is embedded in the top surface of the outer cup body, and the top surface of the collecting filter tank is provided with an air leakage hole and a through hole for connecting with the collecting pipe; a flow storage gap is provided between the collecting filter tank and the outer cup body; the intercepting filter unit includes a first inner filter tank, a first collecting tank is provided outside the first inner filter tank; a first docking plug is provided on the first collecting tank; the first docking plug is docked with the first inner filter tank; the upper filter and the lower filter include a second inner filter tank, a second collecting tank is provided outside the second inner filter tank; a drain port is provided at the bottom of the second collecting tank; the bottom of the second collecting tank is connected to the air pressure valve; a second docking plug is provided on the top of the second collecting tank; the first collecting tank and the second collecting tank include a tank body, and a cover body is screwed on the top of the tank body.

7. The tumor cell cluster separation and filtration device for oncology according to claim 6, characterized in that: The collecting filter tank, the first inner filter tank and the second inner filter tank all include an outer filter cartridge and an inner filter cartridge, and a filter membrane is sandwiched between the outer filter cartridge and the inner filter cartridge; the pore size of the filter membrane of the collecting filter tank and the second inner filter tank is 8~15μm; the pore size of the filter membrane of the first inner filter tank is 50~80μm.

8. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The pneumatic valve includes a valve tube with a three-way structure, a valve core cavity is provided in the middle of the valve tube, and the upper and lower ends of the valve tube are the liquid inlet and the liquid outlet; the middle end of the valve tube is movably provided with a valve core, the valve core and the valve core cavity are movably engaged, a first spring body is movably engaged on the inner side of the valve core, a fixed core column is fixed on the inner side of the valve core cavity, the fixed core column is embedded in the valve core cavity, and abuts against the first spring body; an air pressure tube is screwed on the middle end of the valve tube; the end of the air pressure tube abuts against the valve core.

9. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: A plurality of baffles are arranged inside the slow-flow bucket; the plug connector includes a hose section connected to the bottom of the slow-flow bucket, and a plug is connected to the bottom of the hose section; a second spring body is arranged between the plug and the slow-flow bucket.

10. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: A liquid level sensing unit is provided at the lower part of the venturi ejector.

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