Intelligent co-culture dish with adjustable counterweight

Abstract

The application discloses a weight-adjustable intelligent confocal culture dish and relates to the technical field of biological culture equipment. A limiting support ring is arranged on the inner side of a processing fixed barrel, a plurality of clamping limiting columns are equidistantly welded at the top end of the limiting support ring, a fixed support disc is sleeved at the top end of the limiting support ring, a plurality of insertion connecting ports are equidistantly arranged at the top end of the fixed support disc, a clamping thread groove is arranged on the outer side of the processing fixed barrel, a clamping thread ring is threadedly connected to the top of the outer side of the processing fixed barrel, an operation combination ring is rotatably connected to the inner side of the clamping thread ring, and a limiting support box is equidistantly welded to the bottom end of the operation combination ring. The application effectively solves the problem that the culture dish cannot be separated and taken in the prior art, the internal environment of the culture dish is greatly changed when the culture dish is taken and observed, the stability of culture is affected, the inside and outside are isolated, the influence of the external environment on the culture environment is reduced, and the stability of culture is improved.

Description

Technical Field

[0001] This invention relates to the field of biological culture equipment technology, specifically to an intelligent confocal culture dish with adjustable weight. Background Technology

[0002] A petri dish is a laboratory vessel used for culturing microorganisms or cells. It consists of a flat, disc-shaped base and a lid, and is generally made of glass or plastic. It is available in disposable and reusable forms and is suitable for laboratory inoculation, streaking, and bacterial isolation. In confocal microscopy, a point light source illuminates the sample in the imaging system, and the light carrying the sample information is collected by a point detector. Finally, lateral and axial scanning techniques are used to obtain the three-dimensional information of the entire sample.

[0003] However, during the use of petri dishes, the biological liquid in the petri dishes needs to be placed in a fixed culture environment for cultivation. This means that during observation, samples need to be taken from the culture environment and placed into the confocal petri dish for observation. During the sampling operation, due to the change of environment and the need to use equipment for handling, the sampled organisms are prone to death or overall damage under the influence of handling and environment, which greatly affects the accuracy of observation. Summary of the Invention

[0004] This invention provides an intelligent confocal culture dish with adjustable weight, which can effectively solve the problems mentioned in the background art, such as the change in environment after the culture dish is removed during use, which greatly affects the subsequent normal culture, and the lack of isolation between the inlet and outlet air exchanges, which leads to mutual communication between environments and further affects the culture environment.

[0005] To achieve the above objectives, the present invention provides the following technical solution: an intelligent confocal culture dish with adjustable counterweight, comprising a processing fixing tank, wherein the processing fixing tank is provided with operating components;

[0006] The operating components include a limiting support ring;

[0007] A limiting support ring is installed inside the fixed barrel. Several locking limiting posts are welded at equal intervals to the top of the limiting support ring. A fixed support plate is sleeved on the top of the limiting support ring. Several insertion connection ports are opened at equal intervals on the top of the fixed support plate.

[0008] The outer end of the processing fixed barrel is provided with a locking thread groove. The top of the outer side of the processing fixed barrel is connected to a locking thread ring by a thread. An operating combination ring is rotatably connected to the inner side of the locking thread ring. Several limiting support boxes are welded at equal intervals to the bottom end of the operating combination ring. Several compression springs are welded at equal intervals to the top of the inner side of the limiting support boxes. A pressing processing box is welded to the bottom end of the multiple compression springs. An anti-slip processing pad is glued to the bottom end of the pressing processing box.

[0009] Several inner hole damping blocks are welded at equal intervals to the outer end of the locking threaded ring. A combined processing column is sleeved on the inner side of the inner hole damping block, and a limiting fit frame is welded to the top of the multiple combined processing columns.

[0010] According to the above technical solution, the locking and limiting post is sleeved inside the insertion connection port, and the longitudinal section of the fixed support plate is T-shaped.

[0011] According to the above technical solution, the top of the side end of the fixed support plate is attached to the inner end of the processing fixed barrel, and the bottom end of the operation combination ring is attached to the top of the processing fixed barrel.

[0012] According to the above technical solution, the pressing treatment box and the limiting support box are slidably fitted together, and the bottom end of the anti-slip treatment pad is attached to the top end of the fixed support plate.

[0013] According to the above technical solution, the cross-section of the anti-slip pad is arc-shaped, and the bottom end of the limiting fit bracket is engaged with the top end of the engaging threaded ring.

[0014] According to the above technical solution, the inner side of the limiting frame is rotatably connected with a locking limit cover, and a sealing ring is glued to the bottom outer side of the locking limit cover;

[0015] The top of the locking and limiting cover is equidistantly connected to several exhaust fixing pipes, one end of each of the exhaust fixing pipes is connected to a filter purification bucket, and the top of the filter purification bucket is connected to an inlet and outlet operation pipe.

[0016] According to the above technical solution, the filter purification bucket is snapped onto the top of the locking limit cover, and the side end of the sealing fixing ring is respectively attached to the side end of the limiting fitting frame and the side end of the operating combination ring.

[0017] According to the above technical solution, the processing fixing bucket is provided with a matching component;

[0018] The mating assembly includes a limiting magnetic ring;

[0019] A limiting magnetic ring is sleeved on the outer end of the fixed barrel. A connecting frame is rotatably connected to the top of the limiting magnetic ring. Several counterweights are equidistantly sleeved on the inner side of the connecting frame. Several support buffer blocks are equidistantly bonded to the inner end of the connecting frame.

[0020] A permanent magnet ring is fixed in the middle of the inner side of the locking and limiting cover. A connecting magnetic ring is magnetically connected to the bottom of the permanent magnet ring. An isolation mesh plate is fixed at the top and bottom of the inner side of the connecting magnetic ring. An irregularly shaped air inlet hole is opened at the top of the inner side of the locking and limiting cover. A multi-hole sealing ring is rotatably connected to the top of the inner side of the irregularly shaped air inlet hole.

[0021] According to the above technical solution, the outer diameter of the connecting magnetic ring is equal to the inner diameter of the locking and limiting cover, and there are two isolation mesh plates.

[0022] According to the above technical solution, the longitudinal section of the counterweight is T-shaped, and two adjacent counterweights fit together.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0024] 1. Equipped with an operating component, the fixed support plate is secured to the top of the limiting support ring via a locking retaining post and an insertion connector, enabling the placement and removal of the fixed support plate. The locking threaded groove connects the locking threaded ring and the processing fixed bucket, securing the operating assembly ring to the top of the processing fixed bucket. A compression spring then moves the lower processing box and anti-slip pad down to the top of the fixed support plate, pressing it in place and ensuring its stability. This allows for the easy placement and removal of culture dishes during cultivation and observation, preventing cultivation abnormalities caused by changes in the cultivation environment that cannot be quickly reversed. In this case, the limiting frame and locking cap are fixed to the top of the processing tank by combining the processing column and the inner hole damping block. The inlet and outlet operating pipes and the exhaust fixed pipe allow bidirectional air intake and exhaust. The filter and purification tank filters the intake and exhaust air, reducing the possibility of gas cross-contamination and environmental impact due to lack of isolation. This effectively solves the problem in the existing technology where the culture dish is a whole and cannot be separated for use, resulting in significant changes to the internal environment of the culture dish during use and observation, which affects the stability of the culture. At the same time, the internal and external isolation treatment reduces the impact of the external environment on the culture environment and improves the stability of the culture.

[0025] 2. Equipped with a mating assembly, the connecting magnetic ring is magnetically fixed by a permanent magnet ring. The isolation mesh plate is then fitted inside the locking and limiting cover to achieve mesh plate connection. This allows for quick equipment replacement during isolation and protection. By rotating the connecting mating frame, the position of the counterweight is switched, and the limiting magnetic ring magnetically engages the connecting mating frame. The counterweight block is placed inside the connecting mating frame as needed to achieve counterweight processing and adjustment. This allows for adjustment based on the flatness of the liquid surface during microscope observation and culture, ensuring stable observation and culture. By rotating the porous sealing ring, the air intake and exhaust size of the irregularly shaped air inlet is adjusted to achieve air intake and exhaust adjustment, ensuring stable air intake and exhaust. Utilizing multiple sets of adjustment and linkage, the levelness of the liquid surface in the culture dish is adjusted, improving the stability of culture and the accuracy of observation.

[0026] In summary, by cooperating with the operating components and using multi-position separation processing in the culture dish, culture abnormalities caused by excessive environmental changes can be reduced during sampling observation and continuous culture. At the same time, by adjusting the counterweight and isolating the air intake, liquid level control and culture environment control can be achieved, thereby effectively improving the stability of culture and the accuracy of observation. Attached Figure Description

[0027] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0028] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0029] Figure 2 This is a schematic diagram of the structure of the operating components of the present invention;

[0030] Figure 3 This is a schematic diagram of the installation structure of the locking and limiting post of the present invention;

[0031] Figure 4 This is a schematic diagram of the installation structure of the limiting support box of the present invention;

[0032] Figure 5 This is a schematic diagram of the installation structure of the locking and limiting cover of the present invention;

[0033] Figure 6 This is a schematic diagram of the structure of the mating components of the present invention;

[0034] Figure 7 This is a schematic diagram of the installation structure of the irregular-shaped air inlet of the present invention;

[0035] Figure 8 This is a schematic diagram of the installation structure of the counterweight block of the present invention;

[0036] The diagram shows: 1. Processing the fixed bucket;

[0037] 2. Operating components; 201. Restriction support ring; 202. Engaging restriction post; 203. Fixed support plate; 204. Insertion connection port; 205. Engaging threaded groove; 206. Engaging threaded ring; 207. Operating combination ring; 208. Limiting support box; 209. Compression spring; 210. Downward pressing box; 211. Anti-slip pad; 212. Inner hole damping block; 213. Combination processing post; 214. Restriction mating frame; 215. Engaging limit cover; 216. Sealing fixing ring; 217. Exhaust fixing pipe; 218. Filter purification tank; 219. Inlet / outlet operating pipe;

[0038] 3. Matching components; 301. Limiting magnetic ring; 302. Connecting frame; 303. Counterweight; 304. Supporting buffer block; 305. Permanent magnet ring; 306. Connecting magnetic ring; 307. Isolation mesh plate; 308. Irregularly shaped air inlet; 309. Multi-hole sealing ring. Detailed Implementation

[0039] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0040] Example: Figure 1-8 As shown, the present invention provides a technical solution, an intelligent confocal culture dish with adjustable counterweight, including a processing and fixing tank 1, and the processing and fixing tank 1 is provided with an operating component 2;

[0041] Operating component 2 includes a limiting support ring 201, a locking limiting post 202, a fixed support plate 203, an insertion connection port 204, a locking threaded groove 205, a locking threaded ring 206, an operating combination ring 207, a limiting support box 208, a compression spring 209, a downward pressure treatment box 210, an anti-slip treatment pad 211, an inner hole damping block 212, a combination treatment post 213, a limiting mating frame 214, a locking limiting cover 215, a sealing fixing ring 216, an exhaust fixing pipe 217, a filter purification bucket 218, and an inlet / outlet operating pipe 219;

[0042] A limiting support ring 201 is installed inside the processing fixed barrel 1. Several locking limiting posts 202 are welded at equal intervals to the top of the limiting support ring 201. The locking limiting posts 202 are sleeved inside the insertion connection port 204 to achieve a stable connection support and ensure the stability of the overall alignment limitation. A fixed support plate 203 is sleeved on the top of the limiting support ring 201. The longitudinal section of the fixed support plate 203 is T-shaped. The top of the side end of the fixed support plate 203 fits against the inner end of the processing fixed barrel 1 to achieve a sleeve connection and ensure the stability of the alignment limitation. Several insertion connection ports 204 are opened at equal intervals on the top of the fixed support plate 203.

[0043] The outer end of the fixed barrel 1 is provided with a locking thread groove 205. The top of the outer side of the fixed barrel 1 is connected by a locking thread ring 206 through a thread. The inner side of the locking thread ring 206 is rotatably connected to an operating combination ring 207. The bottom end of the operating combination ring 207 is attached to the top end of the fixed barrel 1 to achieve alignment support and load-bearing fixation. Several limiting support boxes 208 are welded at equal intervals to the bottom end of the operating combination ring 207. Several compression springs 209 are welded at equal intervals to the top of the inner side of the limiting support box 208. The bottom end of the multiple compression springs 209 is welded to a pressing processing box 210. The pressing processing box 210 slides and fits with the limiting support box 208. The bottom end of the anti-slip processing pad 211 is attached to the top end of the fixed support plate 203. The cross-section of the anti-slip processing pad 211 is arc-shaped, which makes it stable when fixing and locking the fixed support plate 203 and limiting support, reducing the shaking of the equipment during use. The bottom end of the pressing processing box 210 is glued with the anti-slip processing pad 211.

[0044] Several inner hole damping blocks 212 are welded at equal intervals on the outer end of the engaging threaded ring 206. A combined processing column 213 is sleeved on the inner side of the inner hole damping block 212. A limiting engagement frame 214 is welded to the top of the multiple combined processing columns 213. The bottom end of the limiting engagement frame 214 is engaged with the top end of the engaging threaded ring 206 to achieve a stable connection limitation. An engaging limit cover 215 is rotatably connected to the inner side of the limiting engagement frame 214. A sealing ring 216 is glued to the bottom outer side of the engaging limit cover 215. The side end of the sealing ring 216 is respectively attached to the side end of the limiting engagement frame 214 and the side end of the operating combination ring 207 to ensure the stability of the sealing limitation.

[0045] The top of the locking limit cover 215 is equidistantly connected to several exhaust fixing pipes 217. One end of each exhaust fixing pipe 217 is connected to a filter purification bucket 218. The filter purification bucket 218 is snapped onto the top of the locking limit cover 215. The top of the filter purification bucket 218 is connected to an inlet / outlet operating pipe 219.

[0046] The fixed barrel 1 is equipped with a mating component 3;

[0047] The mating component 3 includes a limiting magnetic ring 301, a connecting mating frame 302, a counterweight block 303, a supporting buffer block 304, a permanent magnet ring 305, a connecting magnetic ring 306, an isolation mesh plate 307, an irregularly shaped air inlet 308, and a porous sealing ring 309.

[0048] A limiting magnetic ring 301 is sleeved on the outer end of the fixed barrel 1. A connecting frame 302 is rotatably connected to the top of the limiting magnetic ring 301. Several counterweights 303 are equidistantly sleeved on the inner side of the connecting frame 302. The longitudinal section of the counterweights 303 is T-shaped. Two adjacent counterweights 303 are nested together to achieve steady counterweight adjustment. Several support buffer blocks 304 are equidistantly bonded to the inner end of the connecting frame 302.

[0049] A permanent magnet ring 305 is fixed in the middle of the inner side of the locking and limiting cover 215. A connecting magnetic ring 306 is magnetically connected to the bottom of the permanent magnet ring 305. An isolation mesh plate 307 is fixed at the top and bottom of the inner side of the connecting magnetic ring 306. The outer diameter of the connecting magnetic ring 306 is equal to the inner diameter of the locking and limiting cover 215. There are two isolation mesh plates 307 to achieve integrated isolation and ensure the stability of the sealing limit and sealing isolation. An irregularly shaped air inlet hole 308 is opened at the top of the inner side of the locking and limiting cover 215. A multi-hole sealing ring 309 is rotatably connected to the top of the inner side of the irregularly shaped air inlet hole 308.

[0050] The working principle and usage process of this invention are as follows: When culturing organisms, the operator drips the liquid of the organism to be cultured onto the inner side of the fixed support plate 203, places the fixed support plate 203 inside the processing fixed tank 1, and moves the insertion connection port 204 on the side end of the fixed support plate 203 down along the locking and limiting post 202, placing the fixed support plate 203 on the top of the limiting support ring 201, thus realizing the placement of the fixed support plate 203;

[0051] The limiting support box 208 and the pressing treatment box 210 are placed inside the processing fixed barrel 1, and the bottom end of the operation combination ring 207 is attached to the top end of the processing fixed barrel 1. By rotating the locking thread ring 206, the locking thread groove 205 is used to connect the locking thread ring 206 and the processing fixed barrel 1, and the operation combination ring 207 is fixed to the top end of the processing fixed barrel 1. The pressing spring 209 drives the pressing treatment box 210 and the anti-slip treatment pad 211 to move down along the limiting support box 208 to the top end of the fixed support plate 203, thereby pressing the fixed support plate 203 to ensure the stability of the fixed support plate 203.

[0052] The permanent magnet ring 305 is used to magnetically fix the connecting magnetic ring 306. The isolation mesh plate 307 is inserted into the inner side of the locking limit cover 215. The combined processing column 213 is inserted into the inner side of the inner hole damping block 212. The limiting frame 214 and the locking limit cover 215 are fixed to the top of the processing fixed barrel 1. The operating combined ring 207, the processing fixed barrel 1 and the locking limit cover 215 are sealed and limited by the sealing ring 216. The position of the counterweight is switched by rotating the connecting frame 302. The limiting magnetic ring 301 magnetically engages the connecting frame 302. The counterweight block 303 is placed inside the connecting frame 302 as needed to achieve counterweight processing and ensure the overall flatness and stability of the counterweight during processing.

[0053] During the cultivation of organisms in the petri dish, gas is injected into the filter purification tank 218 through the inlet / outlet operation pipe 219. The gas passes through the isolation water in the filter purification tank 218 and enters the inner side of the treatment fixed tank 1 through the exhaust fixed pipe 217, adjusting the internal gas environment. The internal air can also enter the inner side of the filter purification tank 218 through the exhaust fixed pipe 217, realizing gas treatment and ensuring the stability of the gas environment in the cultivation. By rotating the porous sealing ring 309, the size of the gas inlet and outlet of the irregular air inlet 308 is adjusted to realize gas adjustment and ensure the stability of gas inlet and outlet.

[0054] The 35mm glass-bottomed confocal culture dish in this application has a glass diameter of 15mm, is circular, and is of standard size. The dish body and lid are made of high-quality polystyrene (PS) material, and the glass bottom is made of high-transparency borosilicate glass. The inner surface of the glass-bottomed dish is TC treated. The glass-bottomed dish combines the convenience of a 35mm culture dish with the imaging advantages of a coverslip. It is suitable for observation scenarios that require high magnification, such as cell observation experiments using laser confocal microscopy, fluorescence microscopy, live cell imaging, phase contrast microscopy, phase contrast interference microscopy, and fluorescence in situ hybridization (FISH), and can easily achieve high-quality imaging.

[0055] Product specifications: Lid diameter 33.61mm, lid height 6.18mm, glass bottom diameter 15mm, glass bottom height 10mm;

[0056] Durable and reliable materials: The body and lid of the culture dish are made of high-quality polystyrene (PS) material, and the bottom surface of the culture dish is TC treated, making it suitable for cell culture;

[0057] High optical performance: The glass base is made of highly transparent borosilicate glass with a high degree of flatness. When paired with a high-resolution microscope, observation and photography are clearer.

[0058] Non-cytotoxic: It is bonded to the culture dish with UV-resistant adhesive, and the glass base is firmly attached to the culture dish, which is non-toxic to cells;

[0059] Rigorous packaging: Produced in a dust-free workshop, sterilized by radiation irradiation, and individually aseptically packaged for simple and convenient use;

[0060] High cleanliness: sterile, enzyme-free, pyrogen-free, and endotoxin-free.

[0061] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A smart co-culture dish with adjustable weight, comprising a processing fixed barrel (1), characterized in that: The processing fixed bucket (1) is equipped with an operating component (2); The operating component (2) includes a limiting support ring (201); The processing fixed barrel (1) is equipped with a limiting support ring (201) on the inner side. Several locking limiting posts (202) are welded at equal intervals to the top of the limiting support ring (201). A fixed support plate (203) is sleeved on the top of the limiting support ring (201). Several insertion connection ports (204) are opened at equal intervals on the top of the fixed support plate (203). The outer end of the processing fixing barrel (1) is provided with a snap-fit ​​threaded groove (205). The top of the outer side of the processing fixing barrel (1) is connected by a snap-fit ​​threaded ring (206) through a thread. The inner side of the snap-fit ​​threaded ring (206) is rotatably connected to an operating combination ring (207). Several limiting support boxes (208) are welded at equal intervals at the bottom end of the operating combination ring (207). Several compression springs (209) are welded at equal intervals at the top end of the inner side of the limiting support box (208). A pressing processing box (210) is welded to the bottom end of the multiple pressing springs (209). An anti-slip processing pad (211) is glued to the bottom end of the pressing processing box (210). The outer end of the snap-fit ​​threaded ring (206) is welded with a number of inner hole damping blocks (212) at equal intervals. The inner hole damping blocks (212) are sleeved with a combined processing column (213). The top of the multiple combined processing columns (213) is welded with a limiting fit frame (214). The inner side of the limiting frame (214) is rotatably connected to a locking limit cover (215), and a sealing ring (216) is glued to the bottom of the outer side of the locking limit cover (215). The top of the locking limit cover (215) is equidistantly connected to several exhaust fixing pipes (217), and one end of each of the exhaust fixing pipes (217) is connected to a filter purification bucket (218). The top of the filter purification bucket (218) is connected to an inlet / outlet operation pipe (219). The processing fixed bucket (1) is provided with a matching component (3); The mating component (3) includes a limiting magnetic ring (301); The outer end of the fixed barrel (1) is fitted with a limiting magnetic ring (301), the top of the limiting magnetic ring (301) is rotatably connected to a connecting frame (302), a number of counterweights (303) are equidistantly fitted on the inner side of the connecting frame (302), and a number of support buffer blocks (304) are equidistantly bonded to the inner end of the connecting frame (302). A permanent magnet ring (305) is fixed in the middle of the inner side of the locking and limiting cover (215). A connecting magnetic ring (306) is magnetically connected to the bottom of the permanent magnet ring (305). An isolation mesh plate (307) is fixed at the top and bottom of the inner side of the connecting magnetic ring (306). A shaped air inlet hole (308) is opened at the top of the inner side of the locking and limiting cover (215). A multi-hole sealing ring (309) is rotatably connected to the top of the inner side of the shaped air inlet hole (308).

2. The smart co-culture dish with adjustable counterweight according to claim 1, wherein, The locking and limiting post (202) is sleeved inside the insertion connection port (204), and the longitudinal section of the fixed support plate (203) is T-shaped.

3. The smart co-culture dish with adjustable counterweight according to claim 1, wherein, The top of the side end of the fixed support plate (203) is attached to the inner end of the processing fixed barrel (1), and the bottom end of the operation combination ring (207) is attached to the top end of the processing fixed barrel (1).

4. The smart co-culture dish with adjustable counterweight according to claim 1, wherein, The pressing treatment box (210) and the limiting support box (208) are slidably fitted together, and the bottom end of the anti-slip treatment pad (211) is attached to the top end of the fixed support plate (203).

5. The smart co-culture dish with adjustable counterweight according to claim 1, wherein, The anti-slip treatment pad (211) has an arc-shaped cross-section, and the bottom end of the limiting fit bracket (214) is engaged with the top end of the engagement threaded ring (206).

6. The smart co-culture dish with adjustable counterweight according to claim 1, wherein, The filter purification bucket (218) is snapped onto the top of the locking limit cover (215), and the side end of the sealing fixing ring (216) is respectively attached to the side end of the limiting fitting frame (214) and the side end of the operation combination ring (207).

7. The smart co-culture dish with adjustable counterweight according to claim 1, wherein, The outer diameter of the connecting magnetic ring (306) is equal to the inner diameter of the locking limit cover (215), and there are two isolation mesh plates (307).

8. The smart co-culture dish with adjustable counterweight according to claim 1, wherein, The longitudinal section of the counterweight (303) is T-shaped, and two adjacent counterweights (303) fit together.

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