Preparation method and application of yeast culture medium containing traditional Chinese medicine residue extract

Abstract

The invention discloses a preparation method and application of a yeast culture medium containing traditional Chinese medicine residue extracts, the yeast culture medium is a yeast liquid culture medium and contains traditional Chinese medicine residue polysaccharide extracts, urea and sodium chloride, and the mass ratio of the traditional Chinese medicine residue polysaccharide extracts to the urea to the sodium chloride is (4-25): (6-40): (2-13). Tests prove that when the culture medium is used for culturing the saccharomycetes, the culture medium is simple in ingredient and low in price, so that the culture medium has good practical application value.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological fermentation, and particularly relates to a yeast culture medium prepared from traditional Chinese medicine residue extract, a preparation method thereof, and an application thereof. Background Art

[0002] Yeast (saccharomyces) is a single-celled organism belonging to higher microbial fungi, and has the characteristics of being harmless and easy to grow.

[0003] Yeast contains a variety of nutrients and has a relatively large cell body, and is widely used in the production of single-cell protein (SCP). The cell protein content of yeast is 30% - 60%, and it is rich in amino acids, meeting the standards of the Food and Agriculture Organization for proteins and amino acids in human nutrition. In addition to providing protein, yeast can also provide B vitamins. Compared with animal protein and plant protein, yeast has a short production cycle, a wide range of raw material sources, high energy utilization efficiency, and little impact on and dependence on the environment, and can be used as an alternative source of animal and plant protein foods. Yeast also contains a variety of vitamins, unsaturated fatty acids, and inorganic salts, and has high nutritional value, and can be used to produce functional foods, and can also be used as a food additive in food processing.

[0004] However, the conventional culture medium components of yeast are complex and expensive, increasing the production cost of yeast. The commonly used yeast culture medium at present is the YPD culture medium, and the main components are glucose, peptone, and yeast extract powder, with a total cost of 25.46 yuan per liter, and the cost is relatively high.

[0005] With the development of traditional Chinese medicine in China, the amount of traditional Chinese medicine waste generated in the process of the traditional Chinese medicine big health industry is increasing day by day. Effectively recycling a large amount of waste residue generated in the industrialization process of traditional Chinese medicine resources in a reasonable manner, enabling it to exert the maximum resource value, improving the resource utilization efficiency, while saving resources and extending the resource economic industrial chain, also plays a role in energy conservation and emission reduction, reducing pollution to the ecological environment and social pressure. Summary of the Invention

[0006] The purpose of the present invention is to promote the comprehensive utilization of traditional Chinese medicine residue and reduce the production and utilization cost of yeast, so as to provide a cheap yeast liquid culture medium using traditional Chinese medicine residue extract to replace the carbon source in the culture medium. It uses low-cost and easily available materials, and can be prepared in a laboratory with general strain culture equipment.

[0007] Another purpose of the present invention is to provide a preparation method of a yeast culture medium containing traditional Chinese medicine residue extract. This method has a streamlined production process and greatly reduces the production cost of the culture medium.

[0008] Another purpose of the present invention is to provide an application of a liquid culture medium containing traditional Chinese medicine residue extract in culturing yeast.

[0009] The object of the present invention can be achieved by the following measures:

[0010] A yeast culture medium containing an extract of traditional Chinese medicine residues is a liquid yeast culture medium, which contains a polysaccharide extract of traditional Chinese medicine residues, urea and sodium chloride, and the mass ratio of the polysaccharide extract of traditional Chinese medicine residues, urea and sodium chloride is 4-25:6-40:2-13.

[0011] The liquid yeast culture medium in the present invention can be composed only of a polysaccharide extract of traditional Chinese medicine residues, urea, sodium chloride and water, or other components can be further added, such as ammonium sulfate, ammonium chloride, etc.

[0012] In a preferred embodiment, in the yeast culture medium of the present invention, the mass ratio of the polysaccharide extract of traditional Chinese medicine residues, urea and sodium chloride is 5-20:10-30:2-10.

[0013] In a preferred embodiment, in the yeast culture medium of the present invention, the mass ratio of the polysaccharide extract of traditional Chinese medicine residues, urea and sodium chloride is 5-15:14-26:3-8.

[0014] In a preferred embodiment, in the yeast culture medium of the present invention, the mass ratio of the polysaccharide extract of traditional Chinese medicine residues, urea and sodium chloride is 8-12:14-18:4-6.

[0015] In a preferred embodiment, in the yeast culture medium of the present invention, the mass ratio of the polysaccharide extract of traditional Chinese medicine residues, urea and sodium chloride is 10:16:5.

[0016] The liquid yeast culture medium of the present invention can be configured into a directly usable liquid culture medium, or can be formulated into a concentrate and diluted before use.

[0017] In a directly usable liquid culture medium, the mass concentration of the polysaccharide extract of traditional Chinese medicine residues, urea and sodium chloride is: the polysaccharide extract of traditional Chinese medicine residues is 0.4-2.5%, urea is 0.6-4%, and sodium chloride is 0.2-1.3%.

[0018] In another directly usable liquid culture medium, the mass concentration of the polysaccharide extract of traditional Chinese medicine residues, urea and sodium chloride is: the polysaccharide extract of traditional Chinese medicine residues is 0.5-2%, urea is 1-3%, and sodium chloride is 0.2-1%.

[0019] In another directly usable liquid culture medium, the mass concentration of the polysaccharide extract of traditional Chinese medicine residues, urea and sodium chloride is: the polysaccharide extract of traditional Chinese medicine residues is 0.5-1.5%, urea is 1.4-2.6%, and sodium chloride is 0.3-0.8%.

[0020] In another directly usable liquid medium, the mass concentrations of the polysaccharide extract from traditional Chinese medicine residue, urea, and sodium chloride are as follows: the polysaccharide extract from traditional Chinese medicine residue is 1%, urea is 1.6%, and sodium chloride is 0.5%.

[0021] The traditional Chinese medicine residue in the present invention can be selected from peony seed meal residue, palm meal residue, wolfberry residue, notoginseng residue, or astragalus residue, preferably peony seed meal residue. Correspondingly, the polysaccharide extract from traditional Chinese medicine residue is one or more of the polysaccharide extract from peony seed meal residue, the polysaccharide extract from palm meal residue, the polysaccharide extract from wolfberry residue, the polysaccharide extract from notoginseng residue, or the polysaccharide extract from astragalus residue, preferably the polysaccharide extract from peony seed meal residue.

[0022] The solvent of the yeast liquid medium in the present invention is water.

[0023] The pH value of the yeast liquid medium in the present invention is natural.

[0024] The present invention provides a preparation method of a yeast medium containing an extract from traditional Chinese medicine residue, which includes the following steps:

[0025] (1) Pretreatment of the fermentation substrate: Crushing the selected traditional Chinese medicine residue and passing it through a sieve.

[0026] (2) Preparation of the fermentation substrate: Taking the pretreated traditional Chinese medicine residue and performing secondary extraction with water.

[0027] (3) Extracting polysaccharides: After the extract is cooled, filtering, collecting the supernatant in the extract, and performing spray drying to obtain the polysaccharide extract from traditional Chinese medicine residue.

[0028] (4) Preparation of the fermentation medium: Mixing the polysaccharide extract from traditional Chinese medicine residue and sodium chloride in water, then sterilizing, cooling, and adding urea or a urea solution that has been sterilized by filtration, and mixing in a laminar flow hood to obtain the product.

[0029] In the preparation method, the sterilization operation can be sterilization at 120 - 125 °C for 15 - 25 min, preferably sterilization at 121 °C for 20 min. The cooling after sterilization generally needs to be cooled to room temperature.

[0030] In the preparation method, the dosage of each component meets the dosage or concentration required in the above content.

[0031] The present invention discloses the application of a yeast medium containing an extract from traditional Chinese medicine residue in culturing yeast.

[0032] In a specific application, it may include the following steps: inoculating the seed liquid at a volume inoculation amount of 4 - 6% with a seed liquid concentration of 10 6 - 10 8Yeast with a concentration of

[0033] 10 6 -10 8 CFU / mL was inoculated into a yeast liquid medium and cultured at 29-31 °C and 150-200 rpm for 48-96 hours.

[0034] In a specific application, it may include the preparation of a yeast seed solution: The activated yeast was inoculated into a YPD liquid medium and cultured in an oscillating incubator to prepare a yeast seed solution, and the concentration of the seed solution was controlled to be 10 6 -10 8 CFU / mL.

[0035] Compared with the prior art, the beneficial effects of the present invention are as follows: The yeast liquid medium and its culture method provided by the present invention have simple components and low prices, can be widely promoted and used on a large scale, and conform to the background of low-carbon economy and circular economy, which helps to achieve the healthy and sustainable development of Chinese medicine residue resources.

[0036] Compared with the traditional culture medium and culture method, the production method of the present invention is simple, the growth condition of yeast is good, and it can be efficiently applied to the culture of yeast.

[0037] The present invention develops a preparation method and application of a yeast medium prepared from an extract of Chinese medicine residues to reduce the production cost of yeast and improve the effect and yield of the medium. This medium uses the soluble polysaccharides extracted from Chinese medicine residues as the main carbon source and optimizes the nitrogen source in the traditional medium for the culture of yeast. It has been proved by experiments that when the medium of the present invention is used to culture yeast, the medium has simple ingredients and low prices, so it has good practical application value.

[0038] Compared with the traditional single-cell protein culture method in the laboratory, the present invention reuses the waste after the extraction of Chinese medicinal materials, obtains the polysaccharide components in Chinese medicine residues, replaces expensive raw materials such as traditional peptone and yeast extract powder, and is used for yeast propagation. It has good proliferation effect, low cost, strong operability and short fermentation time, and is an effective yeast fermentation method. Description of the Drawings

[0039] Figure 1 It is the culture result of yeast on a liquid medium with ammonium dihydrogen phosphate as the nitrogen source.

[0040] Figure 2 It is the culture result of yeast on a liquid medium with diammonium hydrogen phosphate as the nitrogen source.

[0041] Figure 3 It is the culture result of yeast on a liquid medium with ammonium sulfate as the nitrogen source.

[0042] Figure 4 It is the culture result of yeast on a liquid medium with urea as the nitrogen source.

[0043] Figure 5 It is the culture result of yeast on a liquid medium with ammonium chloride as the nitrogen source. Detailed implementation manners

[0044] It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs.

[0045] The present invention will now be further described in conjunction with specific examples. The following examples are only for explaining the present invention and do not limit its content. If the specific experimental conditions are not specified in the examples, they are usually in accordance with conventional conditions or the conditions recommended by the reagent company; for the reagents, consumables, etc. used in the following examples, unless otherwise specified, they can all be obtained from commercial channels.

[0046] The present invention will now be further described in conjunction with specific embodiments and the accompanying drawings. The advantages and features of the present invention will become clearer as the description progresses. However, the embodiments are exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but such modifications and replacements all fall within the protection scope of the present invention.

[0047] The materials involved in each embodiment are as follows:

[0048] 1 Strains and media

[0049] 1.1 Strains

[0050] The test strains are all yeasts.

[0051] Candida utilis, No.: ACCC 20060, purchased from Beijing Biological Collection Center;

[0052] Saccharomyces cerevisiae, No.: ATCC2601, purchased from Shanghai Collection of Biotechnology Center;

[0053] Kluyveromyces marxianus, No.: SHBCC D57014, purchased from Shanghai Collection of Biotechnology;

[0054] Pichia Pastoris, No.: SHBCC D25036, purchased from Shanghai Collection of Biotechnology;

[0055] Candida tropicalis, No.: SZ1, purchased from Beijing Collection of Biology;

[0056] Marine red yeast, No.: SHBCC D25171, purchased from Shanghai Collection of Biotechnology.

[0057] 1.2 Culture medium

[0058] Strain activation medium: 1% yeast powder, 2% peptone, 2% glucose, natural pH.

[0059] Example 1

[0060] The preparation of yeast seed liquid and the preparation of yeast liquid medium respectively include the following steps:

[0061] (1) Preparation of yeast seed liquid: Inoculate the activated yeast into YPD liquid medium and culture it in an oscillating incubator to prepare yeast seed liquid, controlling the seed liquid concentration to be 10 6 ~10 8 CFU / mL;

[0062] (2) Pretreatment of fermentation substrate: Crush the selected peony seed meal, palm meal, wolfberry fruit, notoginseng, and astragalus residue respectively and pass through a sieve;

[0063] (3) Preparation of fermentation substrate: Weigh the peony seed meal, palm meal, wolfberry fruit, notoginseng, and astragalus residue in step (2) respectively according to parts by weight and perform secondary extraction with water.

[0064] (4) After the extract in step (3) is cooled, perform filtration treatment, collect the supernatant in the reaction solution and perform spray drying to obtain the polysaccharide component in the traditional Chinese medicine residue, and set aside;

[0065] (5) Preparation of fermentation medium: Use the polysaccharide of the traditional Chinese medicine residue obtained in step (4) as the carbon source component in the medium, mix sodium chloride in water, make up the volume, sterilize at 121 °C for 20 min and cool to room temperature, filter-sterilize urea, and mix in a clean bench to obtain the yeast liquid medium.

[0066] Example 2

[0067] Prepare the yeast liquid medium by the method of Example 1, where the mass concentration of each component is as follows: polysaccharide extract from peony seed meal residue 1%, urea 1.6%, sodium chloride 0.5%, and the pH value is natural.

[0068] Example 3

[0069] Prepare the yeast liquid medium by the method of Example 1, where the mass concentration of each component is as follows: polysaccharide extract from peony seed meal residue 0.8%, urea 1.8%, sodium chloride 0.5%, and the pH value is natural.

[0070] Example 4

[0071] Prepare the yeast liquid medium by the method of Example 1, where the mass concentration of each component is as follows: polysaccharide extract from peony seed meal residue 1.2%, urea 1.5%, sodium chloride 0.6%, and the pH value is natural.

[0072] Example 5, Screening of carbon sources

[0073] Crush peony seed meal, palm kernel meal, wolfberry fruit, notoginseng, and astragalus residue respectively and pass through a sieve. Weigh peony seed meal, palm kernel meal, wolfberry fruit, notoginseng, and astragalus residue according to parts by weight and perform secondary extraction with water. After the extract is cooled, carry out filtration treatment, collect the supernatant in the reaction solution for spray drying, and obtain the polysaccharide component in the traditional Chinese medicine residue. Determine the polysaccharide content in the secondary extraction of the traditional Chinese medicine residue by the phenol-sulfuric acid method. It can be seen from the data in Table 1 that the polysaccharide extracted from peony seed meal residue has the advantages of high polysaccharide content and high extraction rate. Therefore, the polysaccharide extract from peony seed meal residue is preferred, and it is determined that the carbon source concentration in the fermentation medium is preferably 10 g / L.

[0074] Table 1 Polysaccharide content in the secondary extraction of traditional Chinese medicine residue

[0075]

[0076]

[0077] Example 6, Screening of nitrogen sources

[0078] The following formula is adopted in the yeast liquid medium: polysaccharide extract from peony seed meal residue (carbon source) 1% wt, nitrogen source 1.6%, sodium chloride 0.5% wt, and the pH value is natural.

[0079] Select ammonium chloride, urea, ammonium sulfate, diammonium hydrogen phosphate, and ammonium dihydrogen phosphate as nitrogen sources respectively. According to the components of 1% yeast extract and 2% peptone in the YPD medium, the total nitrogen content in the medium can be obtained to be about 3.8 g / L. To ensure that the nitrogen element content in each medium is equal, calculations are carried out for each nitrogen source:

[0080] Ammonium chloride NH 4 Cl, molecular mass = 14.007(N) + 4 * 1.008(H) + 35.45(Cl) = 53.49 g / mol. There is one nitrogen atom in ammonium chloride, so 0.271 mol of ammonium chloride is needed, which is equivalent to 0.271 mol * 53.49 g / mol = 14.48 g of ammonium chloride. The concentration is 14.48 g / L.

[0081] Urea (NH 2 ) 2 CO, molecular mass = 2 * (14.007(N) + 2 * 1.008(H)) + 12.01(C) + 15.999(O) = 60.06 g / mol. There are two nitrogen atoms in urea, so 0.271 / 2 = 0.136 mol of urea is needed, which is equivalent to 0.136 mol * 60.06 g / mol = 8.15 g of urea. The concentration is 8.15 g / L.

[0082] Ammonium sulfate (NH 4 ) 2 SO 4 , molecular mass = 2 * (14.007(N) + 4 * 1.008(H)) + 32.06(S) + 4 * 15.999(O) = 132.14 g / mol. There are two nitrogen atoms in ammonium sulfate, so 0.271 / 2 = 0.136 mol of ammonium sulfate is needed, which is equivalent to 0.136 mol * 132.14 g / mol = 17.97 g of ammonium sulfate. The concentration is 17.97 g / L.

[0083] Diammonium hydrogen phosphate NH 4 H 2 PO 4 , molecular mass = 14.007(N) + 4 * 1.008(H) + 1.008(H) + 2 * 15.999(O) + 30.974(P) = 80.0 g / mol. There is one nitrogen atom in diammonium hydrogen phosphate, so 0.271 mol of diammonium hydrogen phosphate is needed, which is equivalent to 0.271 mol * 80.0 g / mol = 21.68 g of diammonium hydrogen phosphate. The concentration is 21.68 g / L.

[0084] Ammonium dihydrogen phosphate NH 4 HPO 4 , molecular mass = 14.007(N) + 4 * 1.008(H) + 1.008(H) + 3 * 15.999(O) + 30.974(P) = 96.0 g / mol. There is one nitrogen atom in ammonium dihydrogen phosphate, so 0.271 mol of ammonium dihydrogen phosphate is needed, which is equivalent to 0.271 mol * 96.0 g / mol = 26.02 g of ammonium dihydrogen phosphate. The concentration is 26.02 g / L.

[0085] Sterilize the carbon source and nitrogen source separately. Wait until it cools slightly, then transfer it into the laminar flow hood. Since urea is easily decomposed by heating, filter sterilization is used. Prepare 11 sterile test tubes, each filled with 10 mL of polysaccharide solution. No nitrogen source is added to the blank test tube, 10 mL of ammonium chloride solution is added to test tubes 1 and 2, 10 mL of urea solution is added to test tubes 3 and 4, 10 mL of ammonium sulfate solution is added to test tubes 5 and 6, 10 mL of diammonium hydrogen phosphate solution is added to test tubes 7 and 8, and 10 mL of ammonium dihydrogen phosphate solution is added to test tubes 9 and 10. Then, add 200 μL of bacterial liquid to each test tube, cover the lid, and place it in a constant temperature shaker at 30 °C and 180 rpm for 72 hours. Take it out every 24 hours and change the air in the laminar flow hood (the culture results are as Figures 1 - 5 shown).

[0086] Table 2 Components of each culture medium before fermentation culture

[0087]

[0088]

[0089] Put all the samples into a centrifuge at 4000 rpm for 20 minutes. Pour off the supernatant, only take the precipitate, add pure water equal to the original volume, and vortex on a vortex mixer. Then put it into the centrifuge again for centrifugation. Repeat this step three times to dissolve and remove the small water-soluble sugars in the system. Resuspend the collected solid with the same amount of water. The OD values of the resuspended samples measured at 600 nm again are shown in Table 3:

[0090] Table 3 Growth of yeast in different nitrogen source components

[0091] nitrogen source ammonium chloride urea ammonium sulfate diammonium hydrogen phosphate ammonium dihydrogen phosphate average OD value 0.6493 0.7336 0.6944 0.5393 0.5632

[0092] It can be seen that in the single-factor experiment with a fixed carbon source and only the nitrogen source changed, the growth of yeast is the best in the culture medium with urea as the nitrogen source, followed by ammonium sulfate and ammonium chloride, and the growth of diammonium hydrogen phosphate and ammonium dihydrogen phosphate is the worst. Therefore, urea is used as the preferred nitrogen source component in the culture medium.

[0093] Example 7. Culture method

[0094] Streak the preserved strain onto the activation medium and culture it with shaking at 30 °C and 180 rpm for 72 h.

[0095] Prepare a cheap culture medium for yeast. Mix 1% of polysaccharide extracted from peony seed meal residue and 0.5% of sodium chloride in water according to the formulated amount, make up the volume, sterilize at 121 °C for 20 min and cool to room temperature, and filter sterilize 1.6% of urea. Mix them in the laminar flow hood to obtain the yeast liquid culture medium. According to the inoculation amount of 5% by volume, the seed liquid concentration is 106 ~10 8 Yeast with a concentration of ~10 CFU / mL was inoculated into the cheap yeast medium and cultured at 30 °C and 180 rpm for 72 h.

[0096] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description and ideas. It is not necessary and impossible to list all implementation manners here. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A yeast culture medium containing an extract of traditional Chinese medicine residues, characterized in that it is a yeast liquid culture medium, which contains an extract of traditional Chinese medicine residue polysaccharide, urea and sodium chloride, and the mass ratio of the extract of traditional Chinese medicine residue polysaccharide, urea and sodium chloride is 4-25:6-40:2-13.

2. The yeast culture medium containing an extract of traditional Chinese medicine residues according to claim 1, characterized in that the mass ratio of the extract of traditional Chinese medicine residue polysaccharide, urea and sodium chloride is 5-20:10-30:2-10, preferably 5-15:14-26:3-8, and more preferably 10:16:

5.

3. The yeast culture medium containing an extract of traditional Chinese medicine residues according to claim 1, characterized in that the mass concentrations of the extract of traditional Chinese medicine residue polysaccharide, urea and sodium chloride in the culture medium are: the extract of traditional Chinese medicine residue polysaccharide 0.4-2.5%, urea 0.6-4%, and sodium chloride 0.2-1.3%.

4. The yeast culture medium containing an extract of traditional Chinese medicine residues according to claim 3, characterized in that the mass concentrations of the extract of traditional Chinese medicine residue polysaccharide, urea and sodium chloride in the culture medium are: the extract of traditional Chinese medicine residue polysaccharide 0.5-2%, urea 1-3%, and sodium chloride 0.2-1%, preferably the extract of traditional Chinese medicine residue polysaccharide 0.5-1.5%, urea 1.4-2.6%, and sodium chloride 0.3-0.8%, and more preferably the extract of traditional Chinese medicine residue polysaccharide 1%, urea 1.6%, and sodium chloride 0.5%.

5. The yeast culture medium containing an extract of traditional Chinese medicine residues according to claim 1, characterized in that the extract of traditional Chinese medicine residue polysaccharide is one or more of the extract of peony seed meal residue polysaccharide, palm meal residue polysaccharide, wolfberry residue polysaccharide, notoginseng residue polysaccharide or astragalus residue polysaccharide, preferably the extract of peony seed meal residue polysaccharide; the solvent of the yeast liquid culture medium is water, and the pH value of the yeast liquid culture medium is natural.

6. A preparation method of the yeast culture medium containing an extract of traditional Chinese medicine residues according to claim 1, characterized in that it includes the following steps: (1) Pretreatment of the fermentation substrate: crushing the selected traditional Chinese medicine residues and passing them through a sieve; (2) Preparation of the fermentation substrate: taking the pretreated traditional Chinese medicine residues and performing secondary extraction with water; (3) Extracting polysaccharide: after the extract is cooled, filtering, collecting the supernatant in the extract and performing spray drying to obtain the extract of traditional Chinese medicine residue polysaccharide; (4) Preparation of the fermentation culture medium: mixing the extract of traditional Chinese medicine residue polysaccharide and sodium chloride in water, then sterilizing, cooling and adding urea or a urea solution that has been sterilized by filtration, and mixing in a laminar flow hood to obtain it.

7. The preparation method of the yeast culture medium containing an extract of traditional Chinese medicine residues according to claim 6, characterized in that the sterilization operation is sterilization at 120-125°C for 15-25 minutes.

8. The preparation method of the yeast culture medium containing an extract of traditional Chinese medicine residues according to claim 6, characterized in that The traditional Chinese medicine residue is peony seed meal residue, palm meal residue, wolfberry residue, notoginseng residue or astragalus residue, preferably peony seed meal residue; the mass ratio of the polysaccharide extract of the traditional Chinese medicine residue, urea and sodium chloride in the culture medium is 5-20:10-30:2-10, preferably 5-15:14-26:3-8, and further preferably 10:16:

5.

9. Use of the yeast culture medium containing the extract of traditional Chinese medicine residue according to claim 1 for culturing yeast.

10. According to the use described in claim 9, characterized in that Inoculate the yeast solution with a concentration of 10 6 ~10 8 CFU / mL into the yeast liquid medium at an inoculation volume of 4-6% by volume, and culture at 29-31°C and 150-200 rpm for 48-96 hours.

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