Immunohistochemical staining device

Abstract

The invention relates to an immunohistochemical staining device, which relates to the technical field of biological detection.The immunohistochemical staining device comprises an incubation box body, a plurality of incubation cavities are formed in the incubation box body through partition plates, each incubation cavity is internally provided with a glass slide used for placing a glass slide, a vibration plate used for driving the glass slide to vibrate is arranged below the glass slide, and the glass slide is arranged in the incubation box body. A supporting plate is arranged below the vibrating plate, and a transmission mechanism used for driving the vibrating plate to vibrate is arranged below the supporting plate. A water tank is further arranged in each incubation chamber, a clean water chamber and a buffer solution chamber are arranged in each water tank, clean water dropping from the clean water chambers is used for driving the transmission mechanism to vibrate, and buffer solution dropping from the buffer solution chambers is used for flushing incubated slides. The device integrates an incubation assembly and a flushing assembly, incubation of the section is efficiently completed, auxiliary tiny vibration in the incubation process is beneficial to improving the diffusion and combination efficiency of an antibody on the tissue section, the reaction speed of the antigen and the antibody is increased, combination is more sufficient, and the dyeing effect is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological detection, and specifically relates to an immunohistochemical staining device. Background Art

[0002] Immunohistochemical staining is an important technique in the field of pathology. It uses the specific binding reaction between antigens and antibodies, and through a chemical reaction, a chromogenic agent (such as fluorescein, enzyme, metal ions, etc.) labeled on the antibody is made to develop color to determine the localization, qualitative and quantitative analysis of antigens (such as proteins, polypeptides, enzymes, etc.) in tissue cells, so as to diagnose and study diseases.

[0003] During the existing immunohistochemical staining incubation process, the primary antibody and secondary antibody reagents generally penetrate into the tissue by natural diffusion. However, in this way, the reagents are difficult to evenly cover the surface of the tissue section, and the permeability is not ideal. In order to improve the diffusion and binding efficiency of the antibody on the tissue section, shorten the incubation time, and improve the staining effect, a vibration component is used to assist diffusion. However, the intensity of the vibration component is too intense and easily damages the tissue section. In the existing incubation device during the immunohistochemical staining process, the cleaning of the section after incubation is not convenient and fast enough, and it is difficult to ensure that all the reagents on the section are completely washed away.

[0004] Therefore, we provide an immunohistochemical staining device to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to provide an immunohistochemical staining device for the problems in the background art.

[0006] The present invention achieves the above purpose through the following technical solutions:

[0007] An immunohistochemical staining device includes an incubation box body. Multiple incubation chambers are formed in the incubation box body through partition plates. A glass slide carrier for placing glass slides is provided in each incubation chamber. A vibration plate for driving it to vibrate is provided below the glass slide carrier. A support plate is provided below the vibration plate. A transmission mechanism for driving the vibration plate to vibrate is provided below the support plate. A water tank is also provided in each incubation chamber. A purified water chamber and a buffer solution chamber are provided in the water tank. The purified water dripping in the purified water chamber is used to drive the transmission mechanism to vibrate, and the buffer solution dripping in the buffer solution chamber is used to wash the glass slide after incubation.

[0008] As a further optimized solution of the present invention, a liquid collection tray is provided on the inner bottom surface of the incubation box body. A box door is provided at the front opening of the incubation box body. A box cover and a control member are provided on the top of the incubation box body, and a plurality of indicator lights corresponding to the incubation chambers one by one are also provided on the top of the incubation box body.

[0009] As a further optimized solution of the present invention, a placement groove for placing a glass slide and a water tank for holding the dripping buffer solution are provided at the top of the glass slide carrier; notches are provided on both sides of the placement groove, and elastic clamping blocks for clamping the glass slide are provided in the notches. A plurality of water outlet holes are arranged on the bottom surface of the placement groove. A plurality of flow guide grooves for evenly distributing water flow are arranged at equal intervals between the water tank and the placement groove; a sliding plate is provided below the glass slide carrier, and the glass slide carrier is slidably arranged on the sliding plate. A pulling hole for pushing and pulling the glass slide carrier is provided at the outer end of the top of the glass slide carrier.

[0010] As a further optimized solution of the present invention, one end of the bottom of the sliding plate is hinged to the vibrating plate, and the other end is hinged with an electric telescopic rod for driving it to turn to an inclined flushing state. The other end of the electric telescopic rod is hinged to the vibrating plate.

[0011] As a further optimized solution of the present invention, first springs are fixedly arranged at the four corners of the bottom of the vibrating plate, and the bottom ends of the first springs are fixedly connected to the support plate.

[0012] As a further optimized solution of the present invention, the transmission mechanism includes a mounting seat and a lever rotatably arranged in the mounting seat; one end of the lever is hinged with a vibration transmission rod, the vibration transmission rod passes through the support plate and is fixedly connected to the center of the bottom of the vibrating plate, and a drip plate is fixedly arranged at the other end of the lever.

[0013] As a further optimized solution of the present invention, a first fixing seat is provided below the water tank. A first movable rod passing through the first fixing seat is fixedly arranged at the bottom of the water tank. A second spring is sleeved on the first movable rod between the first fixing seat and the water tank; a first solenoid valve is further provided at the bottom of the water tank. A drip pipe with a plurality of holes is provided on the first solenoid valve. The drip pipe is located directly above the drip plate. A second solenoid valve is provided on the side surface of the water tank. A flushing pipe with a plurality of holes is provided on the second solenoid valve.

[0014] As a further optimized solution of the present invention, it further includes a liquid suction assembly located on the side of the water tank for sucking the buffer solution to further rinse the slide after incubation; the liquid suction assembly includes a liquid storage tank integrally formed with the water tank and a liquid suction pipe, the liquid storage tank is communicated with the buffer solution chamber, a liquid suction port is provided at the top of the liquid storage tank, a closing plate is slidably provided above the liquid suction port, a jack matching the liquid suction port is provided on the closing plate, and a spring column for controlling the closing plate to be in a normally closed state is provided on the side of the closing plate; a fixing rod is further fixed on the top of the liquid storage tank, a placing plate for storing the liquid suction pipe is movably sleeved on the fixing rod, and a fourth spring is sleeved on the fixing rod between the placing plate and the liquid storage tank; a resisting rod is fixed at the bottom of the placing plate, a first wedge-shaped block is fixed at the bottom end of the resisting rod, a second wedge-shaped block is fixed at the end of the closing plate, and when the first wedge-shaped block descends, it squeezes the second wedge-shaped block to push the closing plate to move and open the liquid suction port.

[0015] As a further optimized solution of the present invention, an induction mechanism electrically connected to the control member is provided at the side of the water tank; the induction mechanism includes a second fixing seat fixed on the water tank and a third fixing seat fixed on the inner wall of the incubation box, a second movable rod penetrates through the second fixing seat, a first mounting plate is fixed at the top end of the second movable rod, a first metal sheet is provided on the top of the first mounting plate, a third spring is sleeved on the second movable rod between the first mounting plate and the second fixing seat, a second mounting plate is hinged in the third fixing seat through a torsion spring, a second metal sheet is provided at the bottom of the second mounting plate, and the second metal sheet abuts against the first metal sheet to connect the control member.

[0016] The beneficial effects of the present invention are as follows:

[0017] 1. By setting the glass slide, the vibrating plate, the water tank and the air curtain assembly, the staining device integrates the incubation and rinsing components, efficiently completes the incubation of the section, and the vibrating plate helps to improve the diffusion and binding efficiency of the antibody on the tissue section, speeds up the antigen-antibody reaction speed, shortens the incubation time, makes the binding more sufficient, and improves the staining effect.

[0018] 2. By setting the transmission mechanism, the water tank and the induction mechanism, during the incubation process of the section, the impact force of the water droplets generates minute vibrations, which also avoids damage to the tissue section caused by larger mechanical vibrations. At the same time, the timing device is combined with the vibrating device. On the one hand, the water droplets drive the vibrating plate to vibrate slightly, and on the other hand, as the water weight in the water tank gradually decreases, it triggers the induction mechanism, realizing the timing function.

[0019] 3. By providing a liquid suction assembly, after the initial flushing by the flushing tube, the buffer solution is sucked out by the liquid suction assembly and manually dropped onto the section for more detailed cleaning of the section, ensuring that the staining agent on the section is completely removed, avoiding the influence of staining agent residue on subsequent staining steps, and the suction of the buffer solution is relatively convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a three-dimensional schematic diagram of the overall structure of the present invention;

[0021] Figure 2 is an assembly schematic diagram of the glass slide, vibration plate, support plate, transmission mechanism, water tank and induction mechanism of the present invention;

[0022] Figure 3 is an exploded view of the structure of the glass slide, vibration plate, support plate and transmission mechanism of the present invention Figure 1 ;

[0023] Figure 4 is an exploded view of the structure of the glass slide, vibration plate, support plate and transmission mechanism of the present invention Figure 2 ;

[0024] Figure 5 is a schematic diagram of the structure of the water tank and induction mechanism of the present invention;

[0025] Figure 6 is a schematic diagram of the structure of the liquid suction assembly of the present invention.

[0026] In the figure:

[0027] 1. Incubation box; 101. Liquid collection tray; 102. Box door; 103. Box cover; 104. Control member; 105. Indicator light; 2. Glass slide carrier; 201. Elastic clamping block; 202. Water tank; 203. Flow guide groove; 204. Water outlet hole; 205. Pull hole; 206. Slide plate; 207. Electric telescopic rod; 3. Vibration plate; 301. First spring; 4. Support plate; 5. Driving mechanism; 501. Mounting seat; 502. Lever; 503. Vibration transmission rod; 504. Dripping plate; 6. Water tank; 601. Purified water chamber; 602. Buffer solution chamber; 603. First movable rod; 604. First fixed seat; 605. Second spring; 606. First solenoid valve; 607. Drip pipe; 608. Second solenoid valve; 609. Flushing pipe; 7. Induction mechanism; 701. Second fixed seat; 702. Second movable rod; 703. First mounting plate; 704. First metal sheet; 705. Third spring; 706. Third fixed seat; 707. Second mounting plate; 708. Second metal sheet; 8. Liquid absorption assembly; 801. Liquid storage tank; 802. Sealing plate; 803. Jack; 804. Spring column; 805. Fixed rod; 806. Fourth spring; 807. Placing plate; 808. Liquid absorption pipe; 809. Pushing rod; 810. First wedge block; 811. Second wedge block. Detailed implementation manners

[0028] The following further describes the present application in detail with reference to the accompanying drawings. It is necessary to point out here that the following specific implementation manners are only used to further illustrate the present application and cannot be construed as limiting the protection scope of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application according to the above application content.

[0029] Embodiment 1

[0030] In order to solve the problem that the existing immunohistochemical staining incubation device is difficult to integrate incubation and flushing together, and generally infiltrates into tissues by natural diffusion. However, in this way, the reagent is difficult to evenly cover the surface of tissue sections, the permeability is not ideal enough, the incubation time is relatively long, and the staining effect is not ideal enough. Please refer to Figure 1 - Figure 2 The immunohistochemical staining device provided by the present invention includes an incubation box 1. Multiple incubation chambers are formed in the incubation box 1 through partition plates. A glass slide carrier 2 for placing glass slides is provided in each incubation chamber. A temperature control component for controlling the temperature is provided in the incubation chamber. A vibration plate 3 for driving the vibration of the glass slide carrier 2 is provided below the glass slide carrier 2. A support plate 4 is provided below the vibration plate 3. A transmission mechanism 5 for driving the vibration of the vibration plate 3 is provided below the support plate 4; A water tank 6 for flushing the incubated glass slides is also provided in each incubation chamber.

[0031] On the inner bottom surface of the incubation box body 1, there is a liquid collection tray 101. At the front opening of the incubation box body 1, there is a box door 102. On the top of the incubation box body 1, there are a box cover 103 and a control member 104. The box cover 103 is opened to facilitate the water addition to the water tank 6, and on the top of the incubation box body 1, there are also a plurality of indicator lights 105 corresponding to the incubation chambers one by one.

[0032] As Figure 3 - Figure 4 shown, on the top of the glass slide carrier 2, there is a placement groove for placing the glass slide and a water tank 202 for holding the dripping buffer solution; on both sides of the placement groove, there are notches, and in the notches, there are elastic clamping blocks 201 for clamping the glass slide. The elastic clamping blocks 201 can conveniently fix the glass slide to prevent it from moving during the staining process. On the bottom surface of the placement groove, there are a plurality of water outlet holes 204. Between the water tank 202 and the placement groove, there are a plurality of flow guiding grooves 203 evenly distributed at equal intervals for evenly distributing the water flow; below the glass slide carrier 2, there is a sliding plate 206, and the glass slide carrier 2 is slidably arranged on the sliding plate 206. At the outer end of the top of the glass slide carrier 2, there is a pulling hole 205 for pushing and pulling the glass slide carrier 2; at one end of the bottom of the sliding plate 206, it is hinged to the vibrating plate 3, and at the other end, there is an electric telescopic rod 207 hinged for driving it to turn to the inclined flushing state. The other end of the electric telescopic rod 207 is hinged to the vibrating plate 3. The drawer-type structure composed of the glass slide carrier 2 and the sliding plate 206 facilitates the pulling out of the glass slide carrier 2 to drop the reagent; during flushing, the electric telescopic rod 207 is used to drive the glass slide carrier 2 to turn to the inclined state, and the buffer solution in the water tank 6 drips into the water tank 202 and is evenly distributed to the surface of the glass slide carrier 2 through a plurality of flow guiding grooves 203 to flush the glass slide multiple times.

[0033] As Figure 3 - Figure 4 shown, at the four corners of the bottom of the vibrating plate 3, there are first springs 301 fixedly arranged, and the bottom ends of the first springs 301 are fixedly connected to the support plate 4. The first springs 301 are micro springs, and their spring constant can be set to 10 N / mm, which can provide a stable supporting force and at the same time have a certain vibration effect. The vibrating plate 3 helps to improve the diffusion and binding efficiency of the antibody on the tissue section, accelerate the antigen-antibody reaction speed, shorten the incubation time, make the binding more sufficient, and improve the staining effect.

[0034] Embodiment Two

[0035] On the basis of Embodiment One, in order to solve the problem that in the existing device, in order to improve the diffusion and binding efficiency of the antibody on the tissue section, a vibration component is generally used to assist in diffusion, but the intensity of the vibration component is too intense and it is easy to damage the tissue section, as Figure 4 - Figure 5 shown, in the water tank 6, there are a purified water chamber 601 and a buffer solution chamber 602. The purified water dripping in the purified water chamber 601 is used to drive the transmission mechanism 5 to vibrate, and the buffer solution dripping in the buffer solution chamber 602 is used to flush the incubated glass slide.

[0036] The transmission mechanism 5 includes a mounting base 501 and a lever 502 rotatably disposed within the mounting base 501; one end of the lever 502 is hinged with a vibration transmission rod 503, the vibration transmission rod 503 passes through the support plate 4 and is fixedly connected to the center of the bottom of the vibration plate 3, and a drip plate 504 is fixedly provided at the other end of the lever 502.

[0037] A first fixing seat 604 is provided below the water tank 6, a first movable rod 603 penetrating through the first fixing seat 604 is fixedly provided at the bottom of the water tank 6, and a second spring 605 is sleeved on the first movable rod 603 between the first fixing seat 604 and the water tank 6; a first electromagnetic valve 606 is further provided at the bottom of the water tank 6, a drip pipe 607 with a plurality of holes is provided on the first electromagnetic valve 606, the drip pipe 607 is located directly above the drip plate 504, a second electromagnetic valve 608 is provided on the side surface of the water tank 6, and a flushing pipe 609 with a plurality of holes is provided on the second electromagnetic valve 608.

[0038] During use, the purified water in the holes of the drip pipe 607 drips onto the drip plate 504, impacting the drip plate 504 to drive the lever 502 to vibrate slightly, the lever 502 drives the vibration transmission rod 503 to lift and lower slightly, the vibration transmission rod 503 drives the vibration plate 3 to lift and lower slightly, and under the action of the first spring 301, it vibrates slightly. Using the impact force of water droplets to generate slight vibrations helps to improve the diffusion and binding efficiency of antibodies on tissue sections, speeds up the antigen-antibody reaction rate, shortens the incubation time, makes the binding more sufficient, improves the staining effect, and also avoids damage to tissue sections caused by large mechanical vibrations. Moreover, the drip system can also play a certain role in maintaining the humidity inside the incubation box 1. The vibration intensity is adjustable. According to the requirements of different antibodies, by precisely controlling the volume of water in the water tank 6 and adjusting the water flow rate of the first electromagnetic valve 606, the incubation time can be set more accurately to adapt to various experimental conditions.

[0039] An induction mechanism 7 electrically connected to the control member 104 is provided at the side of the water tank 6; the induction mechanism 7 includes a second fixing seat 701 fixed on the water tank 6 and a third fixing seat 706 fixed on the inner wall of the incubation box 1. A second movable rod 702 penetrates through the second fixing seat 701. A first mounting plate 703 is fixedly provided at the top end of the second movable rod 702. A first metal sheet 704 is provided on the top of the first mounting plate 703. A third spring 705 is sleeved on the second movable rod 702 between the first mounting plate 703 and the second fixing seat 701. A second mounting plate 707 is hinged in the third fixing seat 706 through a torsion spring. A second metal sheet 708 is provided at the bottom of the second mounting plate 707. The second metal sheet 708 abuts against the first metal sheet 704 to turn on the control member 104.

[0040] During use, as the weight of the water in the water tank 6 gradually decreases, the water tank 6 gradually rises, driving the second fixed seat 701 to rise. The second fixed seat 701 drives the second movable rod 702, the first mounting plate 703 and the first metal sheet 704 to rise. When the first metal sheet 704 contacts the second metal sheet 708, the circuit of the control member 104 is connected. The control member 104 controls the electric telescopic rod 207 to drive the glass slide carrier 2 to turn to an inclined state and starts to wash by activating the second solenoid valve 608. As the washing progresses, since the weight of the water in the water tank 6 gradually decreases, it continues to rise. At this time, under the pressure of the first mounting plate 703, the second mounting plate 707 rotates, and the first metal sheet 704 separates from the second metal sheet 708 to stop washing, and controls the indicator light 105 to emit an indication signal; the setting of the sensing mechanism 7 enables the water tank 6 to have both a timing function and a vibration driving function. On the one hand, it drives the vibration plate 3 to vibrate slightly through water droplets, and on the other hand, the water tank 6 rises as the weight of the water in it gradually decreases to trigger the sensing mechanism 7, realizing the timing function and avoiding the control circuit being in an on state all the time, with better energy saving performance.

[0041] Embodiment Three

[0042] On the basis of Embodiment One and Embodiment Two, in order to further improve the cleaning effect and ensure the staining quality and section clarity, as Figure 6 shown, it further includes a liquid suction assembly 8 located on the side of the water tank 6 for sucking the buffer solution to further wash the glass slide after incubation is completed. After the flushing tube 609 completes the preliminary flushing, the buffer solution in the buffer solution chamber 602 is sucked out through the liquid suction assembly 8 and manually dropped on the section to perform a more detailed cleaning of the section, ensuring that the staining agent on the section is completely removed and avoiding the influence of staining agent residue on subsequent staining steps.

[0043] The liquid suction assembly 8 includes a liquid storage tank 801 integrally formed with the water tank 6 and a liquid suction tube 808. The liquid storage tank 801 is communicated with the buffer solution chamber 602. The top of the liquid storage tank 801 is provided with a liquid suction port. A closing plate 802 is slidably arranged above the liquid suction port. The closing plate 802 is provided with a jack 803 matching the liquid suction port. A spring column 804 for controlling the closing plate 802 to be in a normally closed state is arranged on the side of the closing plate 802; a fixed rod 805 is also fixedly arranged on the top of the liquid storage tank 801. A placement plate 807 for storing the liquid suction tube 808 is movably sleeved on the fixed rod 805. A fourth spring 806 is sleeved on the fixed rod 805 between the placement plate 807 and the liquid storage tank 801; a resisting rod 809 is fixedly arranged at the bottom of the placement plate 807. A first wedge-shaped block 810 is fixedly arranged at the bottom end of the resisting rod 809. A second wedge-shaped block 811 is fixedly arranged at the end of the closing plate 802. When the first wedge-shaped block 810 descends, it squeezes the second wedge-shaped block 811 to push the closing plate 802 to move and open the liquid suction port.

[0044] When it is necessary to suck the buffer solution in the liquid storage tank 801 through the pipette 808, press down the pipette 808. The pipette 808 drives the placement plate 807 to press down. At this time, the fourth spring 806 is compressed. During the downward pressing process of the placement plate 807, the abutting rod 809 and the first wedge block 810 are driven to move. The first wedge block 810 exerts pressure on the second wedge block 811 to push the closing plate 802 to move horizontally. At this time, the spring column 804 is compressed, and the jack 803 coincides with the liquid suction port to open the liquid storage tank 801. Continue to press down the pipette 808, and the pipette 808 is inserted into the liquid storage tank 801 through the jack 803 and the liquid suction port to suck the buffer solution. After the suction is completed, release the downward pressure of the pipette 808. Under the restoring force of the fourth spring 806 and the spring column 804, the entire liquid suction assembly 8 returns to the initial state. Removing the pipette 808 on the placement plate 807 can perform more refined cleaning on the slices.

[0045] The above-described embodiments merely represent one implementation manner of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patent of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations and improvements can still be made, and these all belong to the protection scope of the present invention.

Claims

1. An immunohistochemical staining device, comprising an incubation box (1), characterized in that: A plurality of incubation chambers are formed in the incubation box (1) by partitions, each of the incubation chambers is provided with a glass slide (2) for placing a glass slide, a vibration plate (3) for driving the glass slide to vibrate is provided below the glass slide (2), a support plate (4) is provided below the vibration plate (3), and a transmission mechanism (5) for driving the vibration plate (3) to vibrate is provided below the support plate (4); Each incubation chamber is also provided with a water tank (6), wherein the water tank (6) is provided with a clean water chamber (601) and a buffer chamber (602), wherein the clean water dripping from the clean water chamber (601) is used to drive the transmission mechanism (5) to vibrate, and the buffer chamber (602) is used to rinse the glass slides after incubation.

2. An immunohistochemical staining device according to claim 1, characterized in that: A liquid collecting tray (101) is provided on the inner bottom surface of the incubation box (1), a box door (102) is provided at the front opening of the incubation box (1), a box cover (103) and a control component (104) are provided on the top of the incubation box (1), and a plurality of indicator lights (105) corresponding to the incubation chambers are also provided on the top of the incubation box (1).

3. An immunohistochemical staining device according to claim 1, characterized in that: The top of the glass slide (2) is provided with a placement groove for placing a glass slide and a water groove (202) for containing dripping buffer solution, notches are provided on both sides of the placement groove, elastic clamping blocks (201) for clamping the glass slide are provided in the notches, a plurality of water outlet holes (204) are arranged on the bottom surface of the placement groove, and a plurality of guide grooves (203) for evenly distributing water flow are arranged between the water groove (202) and the placement groove at equal intervals; A slide plate (206) is provided below the glass carrier (2), the glass carrier (2) is slidably arranged on the slide plate (206), and a pulling hole (205) for pushing and pulling the glass carrier (2) is provided at the top outer end of the glass carrier (2).

4. An immunohistochemical staining device according to claim 3, characterized in that: One end of the bottom of the slide plate (206) is hinged to the vibration plate (3), and the other end is hinged to an electric telescopic rod (207) for driving it to a tilted flushing state, and the other end of the electric telescopic rod (207) is hinged to the vibration plate (3).

5. The immunohistochemical staining device according to claim 1, characterized in that: First springs (301) are fixedly provided at the four corners of the bottom of the vibration plate (3), and the bottom end of the first spring (301) is fixedly connected to the support plate (4).

6. The immunohistochemical staining device according to claim 1, characterized in that: The transmission mechanism (5) comprises a mounting seat (501) and a lever (502) rotatably arranged in the mounting seat (501); A vibration transmission rod (503) is hingedly provided at one end of the lever (502), and the vibration transmission rod (503) passes through the support plate (4) and is fixedly connected to the bottom center of the vibration plate (3). A drip plate (504) is fixedly provided at the other end of the lever (502).

7. An immunohistochemical staining device according to claim 6, characterized in that: A first fixed seat (604) is provided below the water tank (6), a first movable rod (603) penetrating the first fixed seat (604) is fixedly provided at the bottom of the water tank (6), and a second spring (605) is sleeved on the first movable rod (603) between the first fixed seat (604) and the water tank (6); A first solenoid valve (606) is also provided at the bottom of the water tank (6), and a drip pipe (607) having a plurality of holes is provided on the first solenoid valve (606), and the drip pipe (607) is located directly above the drip plate (504). A second solenoid valve (608) is provided on the side of the water tank (6), and a flushing pipe (609) having a plurality of holes is provided on the second solenoid valve (608).

8. The immunohistochemical staining device according to claim 1, characterized in that: It also includes a liquid aspiration component (8) located on the side of the water tank (6) for aspirating a buffer solution to further rinse the glass slide after the incubation is completed; The liquid suction assembly (8) comprises a liquid storage box (801) and a liquid suction tube (808) which are integrally formed with the water tank (6); the liquid storage box (801) is connected to the buffer chamber (602); a liquid suction port is provided on the top of the liquid storage box (801); a closing plate (802) is slidably provided above the liquid suction port; a plug hole (803) matching the liquid suction port is provided on the closing plate (802); a spring column (804) for controlling the closing plate (802) to be in a normally closed state is provided on the side of the closing plate (802); A fixing rod (805) is also fixedly provided on the top of the liquid storage tank (801), a placement plate (807) for storing a pipette (808) is movably sleeved on the fixing rod (805), and a fourth spring (806) is sleeved on the fixing rod (805) between the placement plate (807) and the liquid storage tank (801); A push rod (809) is fixedly provided at the bottom of the placement plate (807), a first wedge block (810) is fixedly provided at the bottom end of the push rod (809), and a second wedge block (811) is fixedly provided at the end of the closing plate (802), and when the first wedge block (810) descends, it squeezes the second wedge block (811) to push the closing plate (802) to move and open the liquid suction port.

9. The immunohistochemical staining device according to claim 2, characterized in that: A sensing mechanism (7) electrically connected to the control element (104) is provided on the side of the water tank (6); The sensing mechanism (7) comprises a second fixing seat (701) fixed on the water tank (6) and a third fixing seat (706) fixed on the inner wall of the incubation box (1); a second movable rod (702) is penetrated through the second fixing seat (701); a first mounting plate (703) is fixedly disposed at the top end of the second movable rod (702); a first metal sheet (704) is disposed at the top of the first mounting plate (703); a third spring (705) is sleeved on the second movable rod (702) between the first mounting plate (703) and the second fixing seat (701); a second mounting plate (707) is hingedly disposed in the third fixing seat (706) via a torsion spring; a second metal sheet (708) is disposed at the bottom of the second mounting plate (707); the second metal sheet (708) contacts the first metal sheet (704) to connect the control component (104).

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