On-site visual fluorescence detection reagent for synthesizing cannabinoid-MDMB

Abstract

The invention relates to an on-site visual fluorescence detection reagent for synthesizing cannabinoid MDMB, the detection reagent is composed of (E)-N '-(2-hydroxy-4-diethylaminobenzene) pyridine formylhydrazine and an organic solvent, (E)-N'-(2-hydroxy-4-diethylaminobenzene) pyridine formylhydrazine and synthesized cannabinoid MDMB are reacted, under irradiation of a 365 nm ultraviolet lamp, the fluorescence intensity of the synthesized cannabinoid MDMB can be detected, the fluorescence intensity of the synthesized cannabinoid MDMB can be detected, and the fluorescence intensity of the synthesized cannabinoid MDMB can be detected. The fluorescence of the solution is changed from light brown to yellow green, the detection of the synthetic cannabinoid MDMB is realized, and the response time is lt; 2 s. The detection reagent provided by the invention can be used for rapid preliminary screening and analysis of synthetic cannabinoid MDMB drugs in on-site drug investigation and drug-related cases, and has the characteristics of simple operation, strong anti-interference performance, rapid response, sensitive reaction, convenient visual observation and the like; the reagent provides an effective visual on-site detection technical means for detection of new sperm and live drug synthesized cannabinoid MDMB substances, has a wide application prospect, provides a good research basis for the field of drug detection, and can provide an effective technical means for rapid on-site drug investigation of public security departments.

Description

Technical Field

[0001] The present invention belongs to the field of trace substance analysis, and provides a on-site visual fluorescence detection reagent for synthetic cannabinoid MDMB. Background Art

[0002] Synthetic cannabinoids are substances chemically designed to mimic the effects of natural cannabis, accounting for approximately one-third of the total emerging psychoactive substances in the current market. Different from natural cannabinoids, synthetic cannabinoids have a stronger affinity for CB1 and CB2 receptors, resulting in more intense hallucinogenic effects. Inhaling synthetic cannabinoids can produce a series of adverse effects, including mood disorders such as restlessness or sedation, altered perception, and psychiatric symptoms such as delusional disorder and thought dissociation. In addition, the use of these substances may cause nausea, vomiting, epilepsy, elevated blood pressure, ischemic stroke, suicidal ideation, and sudden death. Synthetic cannabinoid MDMB is a typical and widely abused synthetic cannabinoid drug. Therefore, there is an urgent need to develop specific, sensitive, and accurate on-site visual detection methods for such drugs.

[0003] Currently, scholars at home and abroad have developed a series of methods for the detection of synthetic cannabinoids, mainly including high-performance liquid chromatography-mass spectrometry (HPLC-MS), gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR), surface-enhanced Raman spectroscopy (SERS), and electrochemical methods. However, the above methods still have limitations. For example, they require expensive instruments, long detection times, complex sample pretreatment, and need to be operated by professionals. Summary of the Invention

[0004] The object of the present invention is to provide a on-site visual fluorescence detection reagent for synthetic cannabinoid MDMB. The detection reagent is composed of (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)picolinohydrazide and an organic solvent. By using the reaction of (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)picolinohydrazide with synthetic cannabinoid MDMB, under the irradiation of a 365 nm ultraviolet lamp, the fluorescence of the solution changes from light brown to yellowish green, and the response time is <2 s. The detection reagent described in the present invention can be used for on-site drug seizure and rapid preliminary screening and analysis of synthetic cannabinoid MDMB in drug-related cases. It has the characteristics of simple operation, strong anti-interference ability, rapid response, sensitive reaction, and convenient visual observation. This reagent provides an effective visual on-site detection technical means for the detection of the new psychoactive substance synthetic cannabinoid MDMB, has broad application prospects, and at the same time provides a good research basis for the field of drug detection, and can provide an effective technical means for the rapid on-site seizure of drugs by the public security department.

[0005] The on-site visual fluorescence detection reagent for synthetic cannabinoid MDMB described in the present invention is carried out according to the following steps:

[0006] Preparation of detection reagent:

[0007] a. Add 4 - diethylamino - 2 - hydroxybenzaldehyde and 2 - pyridinecarboxylic acid hydrazide to 20 mL of absolute ethanol in a molar ratio of 1:1, and react at 85 °C for 12 h to obtain a mixture;

[0008] b. After the reaction is completed, cool the mixture in step a to room temperature, and yellow precipitate will form. Filter to obtain the crude product;

[0009] c. Wash the crude product obtained in step b 5 times with absolute ethanol to obtain a solid, and then vacuum - dry the solid at 40 °C for 12 h to obtain the solid probe (E) - N'-(2 - hydroxy - 4 - diethylaminobenzylidene)pyridinecarboxylic acid hydrazide;

[0010] d. Dissolve the solid probe (E) - N'-(2 - hydroxy - 4 - diethylaminobenzylidene)pyridinecarboxylic acid hydrazide obtained in step c in absolute ethanol or absolute methanol, and ultrasonically dissolve and dilute to obtain a (E) - N'-(2 - hydroxy - 4 - diethylaminobenzylidene)pyridinecarboxylic acid hydrazide fluorescence detection reagent with a concentration of 1 mM;

[0011] Preparation of analyte solution:

[0012] e. Prepare a standard solution of synthetic cannabinoid MDMB with a concentration of 1 mg / mL using methanol;

[0013] Fluorescence detection of MDMB - type synthetic cannabinoids:

[0014] f. Add 100 μL of the detection reagent obtained in step d to a test bottle, add 100 μL of the synthetic cannabinoid MDMB standard solution prepared in step e, and add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, a change from light brown to yellow - green can be observed within 2 s.

[0015] A on - site visual fluorescence detection reagent for synthetic cannabinoid MDMB according to the present invention, the chemical name of this reagent is (E) - N'-(2 - hydroxy - 4 - diethylaminobenzylidene)pyridinecarboxylic acid hydrazide, which is obtained by the reaction of 4 - diethylamino - 2 - hydroxybenzaldehyde and 2 - pyridinecarboxylic acid hydrazide, and the chemical structural formula (I) is:

[0016]

[0017] A on - site visual fluorescence detection reagent for synthetic cannabinoid MDMB according to the present invention, the synthetic cannabinoid MDMB for on - site visual fluorescence detection by this reagent is purchased from Shanghai Yuansi Standard Substance Technology Co., Ltd., and it includes:

[0018] MDMB-4en-PINACA (methyl 3,3-dimethyl-2-[(1-pent-4-enyl-1H-indazole-3-carbonyl)amino]butanoate);

[0019] 5F-MDMB-PINACA (methyl 2-[[1-(5-fluoropentyl)-1H-indazole-3-carbonyl]amino]-3,3-dimethylbutanoate); MDMB-BUTINACA (methyl 2-[(1-butyl-1H-indazole-3-carbonyl)amino]-3,3-dimethylbutanoate);

[0020] 4F-MDMB-BINACA (methyl 2-[[1-(4-fluorobutyl)-1H-indazole-3-carbonyl]amino]-3,3-dimethylbutanoate);

[0021] 5F-MDMB-BICA (methyl 2-[[1-(5-fluoropentyl)-1H-indole-3-carbonyl]amino]-3,3-dimethylbutanoate); MDMB-CHMICA (methyl 2-[[1-(cyclohexylmethyl)-1H-indole-3-carbonyl]amino]-3,3-dimethylbutanoate).

[0022] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0023] A on-site visual fluorescence detection reagent for synthesizing cannabinoid MDMB according to the present invention, aiming at the structural feature of cannabinoid MDMB - having two carbonyl groups, using the terminal chain dicarbonyl of its molecular structure as the recognition site, designing and preparing a fluorescent probe with phenolic hydroxyl and amide groups as recognition groups, and providing a on-site visual fluorescence detection reagent for synthesizing cannabinoid MDMB. Based on the multiple weak interactions between cannabinoid MDMB and the (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)picolinohydrazide probe, blocking the ESIPT process of the (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)picolinohydrazide probe, charge transfer occurs inside the (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)picolinohydrazide probe, and the fluorescence changes from light brown to yellowish green, achieving the purpose of detecting cannabinoid MDMB, and realizing the rapid, sensitive, specific and visual detection of cannabinoid MDMB. This reagent is simple to prepare, low in cost, fast in response speed, can be directly observed with the naked eye, and has strong anti-interference ability, providing an excellent technical means for the on-site detection of cannabinoid MDMB and a good research basis for the field of detecting new psychoactive substances.

[0024] This reagent has the characteristics of high sensitivity, high specificity, strong anti-interference ability and second-level response, and can meet the low-cost visual detection requirements of cannabinoid MDMB. Brief Description of the Drawings

[0025] Figure 1 Fluorescence spectra and visualization pictures of the detection reagent of the present invention for MDMB-4en-PINACA;

[0026] Figure 2 Fluorescence spectra and visualization pictures of the detection reagent of the present invention for 5F-MDMB-PINACA;

[0027] Figure 3 Fluorescence spectra and visualization pictures of the detection reagent of the present invention for MDMB-BUTINACA;

[0028] Figure 4 Fluorescence spectra and visualization pictures of the detection reagent of the present invention for 4F-MDMB-BINACA;

[0029] Figure 5 Fluorescence spectra and visualization pictures of the detection reagent of the present invention for 5F-MDMB-BICA;

[0030] Figure 6 Fluorescence spectra and visualization pictures of the detection reagent of the present invention for MDMB-CHMICA;

[0031] Figure 7 Visualization pictures of the fluorescence response of the detection reagent of the present invention to a series of concentrations of MDMB-4en-PINACA;

[0032] Figure 8 Fluorescence spectra of the detection reagent of the present invention for a series of concentrations of MDMB-4en-PINACA;

[0033] Figure 9 Specific visualization pictures of the reagent of the present invention for MDMB-4en-PINACA, common drugs, structural analogs and potential coexisting substances;

[0034] Figure 10 Specific fluorescence emission spectra of the reagent of the present invention for MDMB-4en-PINACA, common drugs, structural analogs and potential coexisting substances;

[0035] Figure 11 Anti-interference visualization pictures of the reagent of the present invention for MDMB-4en-PINACA, common drugs, structural analogs and potential coexisting substances;

[0036] Figure 12 Anti-interference fluorescence emission spectra of the reagent of the present invention for MDMB-4en-PINACA, common drugs, structural analogs and potential coexisting substances. Detailed implementation manners

[0037] The present invention will be further described below through specific embodiments, but the invention is not limited to these embodiments.

[0038] Example 1

[0039] Preparation of the detection reagent:

[0040] a. 4-Diethylamino-2-hydroxybenzaldehyde and 2-pyridinecarboxylic acid hydrazide were added to 20 mL of absolute ethanol in a molar ratio of 1:1 and reacted at 85 °C for 12 h.

[0041] b. After the reaction was completed, the reaction solution in step a was cooled to room temperature, and a yellow precipitate was formed. The crude product was obtained by filtration.

[0042] c. The crude product obtained in step b was washed 5 times with absolute ethanol to obtain a solid. The obtained solid was vacuum-dried at 40 °C for 12 h to obtain the solid probe (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)pyridinecarboxylic acid hydrazide.

[0043] 1 H NMR (400 MHz, DMSO-d 6 ) δ 12.17 (s, 1H), 11.58 (s, 1H), 8.71 (d, J = 4.3 Hz, 1H), 8.62 (s, 1H), 8.11 (d, J = 7.8 Hz, 1H), 8.07–8.02 (m, 1H), 7.67–7.63 (m, 1H), 7.12 (d, J = 8.8 Hz, 1H), 6.27 (dd, J = 8.8, 2.5 Hz, 1H), 6.13 (d, J = 2.4 Hz, 1H), 3.37–3.31 (m, 4H), 1.10 (t, J = 7.0 Hz, 6H). 13 C NMR (101 MHz, DMSO-d 6 ) δ 160.3, 160.1, 151.9, 150.6, 149.9, 148.9, 138.4, 132.3, 127.3, 123.0, 106.9, 104.1, 97.9, 44.2, 13.0. HRMS m / z: [M+H] + calculated for 313.1659; Found 313.1664.

[0044] d. The solid probe (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)pyridinecarboxylic acid hydrazide obtained in step c was dissolved in methanol, and after ultrasonic dissolution and dilution, a (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)pyridinecarboxylic acid hydrazide fluorescence detection reagent with a concentration of 1 mM was obtained.

[0045] Preparation of the analyte solution:

[0046] e. Prepare a standard solution of synthetic cannabinoid MDMB - 4en - PINACA with a concentration of 1 mg / mL using methanol.

[0047] Fluorescence detection of synthetic cannabinoid MDMB - 4en - PINACA:

[0048] f. Add 100 μL of the detection reagent obtained in step d to a test bottle, add 100 μL of the MDMB - 4en - PINACA standard solution prepared in step e, and add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, a change from light brown to yellow - green can be observed within 2 s. Subsequently, measure with a fluorescence spectrometer. At an excitation wavelength of 365 nm, the emission peak at 630 nm in the emission spectrum disappears, and a new peak appears at 526 nm. This phenomenon has a rapid response and obvious fluorescence changes, indicating that the detection system contains synthetic cannabinoid MDMB - 4en - PINACA. The corresponding fluorescence color change and fluorescence spectrogram are as Figure 1 shown.

[0049] Example 2

[0050] Preparation of the detection reagent:

[0051] a. Add 4 - diethylamino - 2 - hydroxybenzaldehyde and 2 - pyridinecarboxylic acid hydrazide to 20 mL of absolute ethanol in a molar ratio of 1:1, and react at 85 °C for 12 h.

[0052] b. After the reaction is completed, lower the reaction solution in step a to room temperature. Yellow precipitate is formed. Filter to obtain the crude product.

[0053] c. Wash the crude product obtained in step b 5 times with absolute ethanol to obtain a solid. Vacuum - dry the obtained solid at 40 °C for 12 h to obtain the solid probe (E) - N'-(2 - hydroxy - 4 - diethylaminobenzylidene)pyridinecarboxylic acid hydrazide.

[0054] d. Dissolve the solid probe (E) - N'-(2 - hydroxy - 4 - diethylaminobenzylidene)pyridinecarboxylic acid hydrazide obtained in step c in absolute ethanol, and ultrasonically dissolve and dilute to obtain a fluorescence detection reagent of (E) - N'-(2 - hydroxy - 4 - diethylaminobenzylidene)pyridinecarboxylic acid hydrazide with a concentration of 1 mM.

[0055] Preparation of the solution to be measured:

[0056] e. Prepare a standard solution of synthetic cannabinoid MDMB - 4en - PINACA with a concentration of 1 mg / mL using methanol.

[0057] Fluorescence detection of synthetic cannabinoid MDMB - 4en - PINACA:

[0058] f. Add 100 μL of the detection reagent obtained in step d into a test bottle, add 100 μL of the MDMB-4en-PINACA standard solution prepared in step e, add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, a change from light brown to yellowish green of the solution can be observed within 2 s; subsequently, measure with a fluorescence spectrometer. At an excitation wavelength of 365 nm, the emission peak at 630 nm in the emission spectrum disappears, and a new peak appears at 526 nm; this phenomenon has a rapid response and obvious fluorescence change, indicating that the detection system contains the synthetic cannabinoid MDMB-4en-PINACA.

[0059] Example 3

[0060] Prepare the detection reagent according to Example 1;

[0061] Prepare the solution to be measured:

[0062] Prepare a standard solution of the synthetic cannabinoid 5F-MDMB-PINACA with a concentration of 1 mg / mL using methanol;

[0063] Fluorescent detection of the synthetic cannabinoid 5F-MDMB-PINACA:

[0064] Add 100 μL of the detection reagent obtained in step d of Example 1 into a test bottle, add 100 μL of the prepared 5F-MDMB-PINACA standard solution at 1 mg / mL, add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, a change from light brown to yellowish green of the solution can be observed within 2 s; subsequently, measure with a fluorescence spectrometer. At an excitation wavelength of 365 nm, the emission peak at 630 nm in the emission spectrum disappears, and a new peak appears at 530 nm; this phenomenon has a rapid response and obvious fluorescence change, indicating that the detection system contains the synthetic cannabinoid 5F-MDMB-PINACA, and the corresponding fluorescence color change and fluorescence spectrogram are as Figure 2 shown.

[0065] Example 4

[0066] Prepare the detection reagent according to Example 1;

[0067] Prepare the solution to be measured:

[0068] Prepare a standard solution of the synthetic cannabinoid MDMB-BUTINACA with a concentration of 1 mg / mL using methanol;

[0069] Fluorescent detection of the synthetic cannabinoid MDMB-BUTINACA:

[0070] Add 100 μL of the detection reagent obtained in step d of Example 1 to a test bottle, add 100 μL of the prepared MDMB-BUTINACA standard solution at 1 mg / mL, add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, a change from light brown to yellow-green can be observed within 2 s. Subsequently, using a fluorescence spectrometer to measure, at an excitation wavelength of 365 nm, the emission peak at 630 nm in the emission spectrum disappears, and a new peak appears at 522 nm. This phenomenon has a rapid response and obvious fluorescence changes, indicating that the detection system contains the synthetic cannabinoid MDMB-BUTINACA. The corresponding fluorescence color change and fluorescence spectrogram are as shown in Figure 3 shown.

[0071] Example 5

[0072] Prepare the detection reagent according to Example 1;

[0073] Prepare the solution to be tested:

[0074] Prepare a standard solution of the synthetic cannabinoid 4F-MDMB-BINACA at a concentration of 1 mg / mL with methanol;

[0075] Fluorescent detection of the synthetic cannabinoid 4F-MDMB-BINACA:

[0076] Add 100 μL of the detection reagent obtained in step d of Example 1 to a test bottle, add 100 μL of the prepared 4F-MDMB-BINACA standard solution at 1 mg / mL, add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, a change from light brown to yellow-green can be observed within 2 s. Subsequently, using a fluorescence spectrometer to measure, at an excitation wavelength of 365 nm, the emission peak at 630 nm in the emission spectrum disappears, and a new peak appears at 531 nm. This phenomenon has a rapid response and obvious fluorescence changes, indicating that the detection system contains the synthetic cannabinoid 4F-MDMB-BINACA. The corresponding fluorescence color change and fluorescence spectrogram are as shown in Figure 4 shown.

[0077] Example 6

[0078] Prepare the detection reagent according to Example 1;

[0079] Prepare the solution to be tested:

[0080] Prepare a standard solution of the synthetic cannabinoid 5F-MDMB-BICA at a concentration of 1 mg / mL with methanol;

[0081] Fluorescent detection of the synthetic cannabinoid 5F-MDMB-BICA:

[0082] Add 100 μL of the detection reagent obtained in step d of Example 1 to a test bottle, add 100 μL of the prepared 5F-MDMB-BICA standard solution at 1 mg / mL, add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, a change from light brown to yellow-green can be observed within 2 s. Subsequently, using a fluorescence spectrometer, at an excitation wavelength of 365 nm, the emission peak at 630 nm in the emission spectrum disappears, and a new peak appears at 532 nm. This phenomenon has a rapid response and obvious fluorescence changes, indicating that the detection system contains the synthetic cannabinoid 5F-MDMB-BICA. The corresponding fluorescence color change and fluorescence spectrogram are as shown in Figure 5 shown.

[0083] Example 7

[0084] Prepare the detection reagent according to Example 1;

[0085] Prepare the solution to be tested:

[0086] Prepare a standard solution of the synthetic cannabinoid MDMB-CHMICA in methanol at a concentration of 1 mg / mL;

[0087] Fluorescence detection of the synthetic cannabinoid MDMB-CHMICA:

[0088] Add 100 μL of the detection reagent obtained in step d of Example 1 to a test bottle, add 100 μL of the prepared MDMB-CHMICA standard solution at 1 mg / mL, add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, a change from light brown to yellow-green can be observed within 2 s. Subsequently, using a fluorescence spectrometer, at an excitation wavelength of 365 nm, the emission peak at 630 nm in the emission spectrum disappears, and a new peak appears at 521 nm. This phenomenon has a rapid response and obvious fluorescence changes, indicating that the detection system contains the synthetic cannabinoid MDMB-CHMICA. The corresponding fluorescence color change and fluorescence spectrogram are as shown in Figure 6 shown.

[0089] Example 8

[0090] Prepare the detection reagent according to Example 1;

[0091] Prepare the solution to be tested:

[0092] Prepare a standard solution of the synthetic cannabinoid MDMB-4en-PINACA in methanol at a concentration of 1 mg / mL;

[0093] Preparation of the standard solution of the synthetic cannabinoid MDMB-4en-PINACA: Take 1 mg / mL of the MDMB-4en-PINACA standard solution and dilute it with methanol to obtain the standard solutions of the synthetic cannabinoid MDMB-4en-PINACA with concentrations of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, and 0.9 mg / mL respectively;

[0094] Fluorescence detection of the synthetic cannabinoid MDMB-4en-PINACA:

[0095] Add 100 μL of the detection reagent obtained in step d of Example 1 to a test bottle, add 100 μL of the prepared standard solutions of the synthetic cannabinoid MDMB-4en-PINACA with a series of concentrations, and add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, within 2 s, it can be observed that as the concentration of the synthetic cannabinoid MDMB-4en-PINACA increases, the color of the detection reagent gradually changes from light brown to yellowish green, and the color gradually deepens; Subsequently, it is measured with a fluorescence spectrometer. At an excitation wavelength of 365 nm, the emission peak at 630 nm in the emission spectrum gradually disappears, and a new peak gradually appears at 526 nm, and the fluorescence intensity shows a gradient increase; The corresponding fluorescence color change is as Figure 7 shown, and the fluorescence spectrogram is as Figure 8 shown.

[0096] Example 9

[0097] Prepare the detection reagent according to Example 1;

[0098] Prepare the solution to be tested:

[0099] Prepare a standard solution of the synthetic cannabinoid MDMB-4en-PINACA with a concentration of 1 mg / mL using methanol;

[0100] Preparation of the solutions of other substances: Select the structural analogs of the synthetic cannabinoid MDMB (indazole, indole, ethyl acetate), potential coexisting substances (propylene glycol, glycerol, acetone, benzene, polyethylene glycol), and common drugs (methamphetamine, cannabis, heroin, ketamine, cocaine, magic mushrooms, opium, ecstasy, morphine, phenobarbital) as the substances to be tested, and prepare standard solutions with a concentration of 10 mg / mL using methanol as the solvent;

[0101] Fluorescence detection of the synthetic cannabinoid MDMB-4en-PINACA:

[0102] Add 100 μL of the detection reagent obtained in step d of Example 1 to a test bottle, add 100 μL of the prepared synthetic cannabinoid MDMB-4en-PINACA solution at 1 mg / mL and 100 μL of the remaining substance solution at 10 mg / mL respectively, add 800 μL of ultrapure water with pH = 7. Under the irradiation of a 365 nm ultraviolet lamp, it can be observed within 2 s that when only synthetic cannabinoid MDMB-4en-PINACA is present, the detection reagent changes from light brown to yellowish green, and when other substances are present, the color of the detection reagent does not change; subsequently, measured with a fluorescence spectrometer, at an excitation wavelength of 365 nm, when only synthetic cannabinoid MDMB-4en-PINACA is present, the emission peak at 630 nm in the emission spectrum disappears, and a new peak appears at 526 nm. When other substances are present, the fluorescence emission intensity at 630 nm does not change, and no new peak appears at 526 nm; the corresponding fluorescence color change is as Figure 9 shown, and the fluorescence spectrogram is as Figure 10 shown.

[0103] The above results show that this detection reagent has good visual discrimination and detection ability for MDMB-4en-PINACA and common drugs, structural analogs and potential coexisting substances.

[0104] Example 10

[0105] Prepare the detection reagent according to Example 1;

[0106] Prepare the solution to be tested:

[0107] Prepare a standard solution of synthetic cannabinoid MDMB-4en-PINACA at a concentration of 1 mg / mL with methanol;

[0108] Preparation of the remaining substance solution: Select structural analogs of the synthetic cannabinoid MDMB type (indazole, indole, ethyl acetate), potential coexisting substances (propylene glycol, glycerol, acetone, benzene, polyethylene glycol), common drugs (methamphetamine, cannabis, heroin, ketamine, cocaine, ecstasy, opium, MDMA, morphine, phenobarbital) as the substances to be tested, and prepare standard solutions at a concentration of 10 mg / mL with methanol as the solvent;

[0109] Fluorescence detection of synthetic cannabinoid MDMB-4en-PINACA:

[0110] Add 100 μL of the detection reagent obtained in step d of Example 1 into a test bottle, add 100 μL of a mixed solution of synthetic cannabinoid MDMB-4en-PINACA at 1 mg / mL and the remaining substances at 10 mg / mL, with a volume ratio of 1:1, add 800 μL of ultrapure water with a pH of 7. Under the irradiation of a 365 nm ultraviolet lamp, a change in the color of all system solutions from light brown to yellowish green can be observed within 2 s; subsequently, measured with a fluorescence spectrometer, at an excitation wavelength of 365 nm, the emission peaks at 630 nm in the emission spectra of all system solutions disappear, and new peaks appear at 526 nm; the corresponding fluorescence color change is as Figure 11 shown, and the fluorescence spectrogram is as Figure 12 shown.

[0111] The above results indicate that the detection phenomenon of this detection reagent for synthetic cannabinoid MDMB-4en-PINACA is not interfered by common drugs, structural analogs, and potential coexisting substances.

Claims

1. A field visualization fluorescent detection reagent for the synthetic cannabinoid MDMB, characterized in that The test reagent is carried out according to the following steps: Prepare detection reagents: a. Add 4-diethylamino-2-hydroxybenzaldehyde and 2-pyridinecarboxylic acid hydrazide to 20 mL of anhydrous ethanol at a molar ratio of 1:1, and react at 85 °C for 12 h to obtain a mixture; b. After the reaction is completed, the mixture in step a is cooled to room temperature, and a yellow precipitate is generated, which is filtered to obtain a crude product; c. The crude product obtained in step b was washed with anhydrous ethanol for 5 times to obtain a solid, and then the solid was vacuum dried at 40° C. for 12 h to obtain a solid probe (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)picolinohydrazide; d. Dissolve the solid probe (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)picolinohydrazide obtained in step c in anhydrous ethanol or anhydrous methanol, and dissolve and dilute by ultrasonication to obtain a (E)-N'-(2-hydroxy-4-diethylaminobenzylidene)picolinohydrazide fluorescence detection reagent with a concentration of 1 mM; Prepare the test solution: e. Prepare the synthetic cannabinoid MDMB into a standard solution with a concentration of 1 mg / mL using methanol; Fluorescence detection of the synthetic cannabinoid MDMB: f. Add 100 µL of the detection reagent obtained in step d into the test bottle, add 100 µL of the synthetic cannabinoid MDMB standard solution prepared in step e, add 800 µL of ultrapure water with a pH of 7, and under 365 nm ultraviolet light, the solution can be observed to change from light brown to yellow-green within 2 seconds.

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