Automatic endotoxin detection device and detection method thereof

Abstract

The invention relates to the technical field of detection devices and detection methods thereof, and provides an automatic endotoxin detection device and a detection method thereof, which can form a plurality of relatively independent reaction spaces, can sequentially form endotoxin detection operations of a plurality of samples while ensuring basic endotoxin detection, and has the advantages of high automation degree and high detection efficiency. The device is more convenient to use, higher in efficiency and smaller in energy consumption and comprises a base, a constant-temperature box and a plurality of carrier boxes, the base is provided with an installation strip opening, the constant-temperature box is provided with two covering pipes, the two covering pipes are communicated with a water inlet pipe and a water return pipe respectively, and a circulating pump and a heater are installed in the base; the water inlet pipe and the water return pipe are communicated with a pump inlet and a pump outlet of the circulating pump respectively, the heater is matched with the water inlet pipe to form coating heating, a partition isolation structure is installed in the constant-temperature box and divides the constant-temperature box into a plurality of placement openings, each carrier box comprises a bottom shell and a top cover, and placement fixing frames are arranged in the bottom shells.

Description

Technical Field

[0001] The present invention relates to the technical field of detection devices and their detection methods, and particularly relates to an endotoxin automatic detection device and its detection method. Background Art

[0002] As is well known, the gel clotting reagent of horseshoe crab is an important biological reagent mainly used for detecting endotoxin. Its working principle is based on the specific reaction between the coagulation protein in the blood of the marine organism "horseshoe crab" and bacterial endotoxin, resulting in the formation of a gel. The speed of this reaction and the firmness of the gel are related to the concentration of endotoxin. By observing the formation of the gel, the content of endotoxin in the bacterial sample can be detected. To facilitate the automatic detection of endotoxin, we propose an endotoxin automatic detection device and its detection method.

[0003] After retrieval, the patent with the Chinese patent publication number CN218646986U and the patent with the Chinese patent publication number CN210442378U respectively disclose a serum endotoxin detection device and an endotoxin detection device for the horseshoe crab reagent method. The former is roughly described as including a base, the base is connected with a detection box, the detection box is connected with a cover body, the cover body is provided with a buckle and an LED lamp, the detection box is provided with a detection plate, a recovery groove, a water bath box and a heating device, the detection plate is provided with a handle and is provided with test tube holes, an arc-shaped clamp is arranged in the test tube holes, the arc-shaped clamp is connected with a connecting rod, the detection plate is provided with a spring hole, the spring hole is connected with a spring, the detection plate is connected with a lifting plate, the bottom of the lifting plate is connected with a hydraulic rod, the base is provided with a control panel, and the control panel is provided with a digital display thermometer, a temperature adjustment knob, a power switch, a light switch, a pause switch, a rise switch and a fall switch. When in use, the temperature adjustment knob is adjusted to control the temperature of the water in the water bath box within a suitable range. The prepared horseshoe crab reagent, the sample to be tested and the comparison sample are added into the test tubes according to certain proportions as required by the experiment. Then the test tubes are respectively placed into the test tube holes. The arc of the arc-shaped clamp fits the size of the test tube, and the test tube is clamped tightly under the elastic force of the spring to prevent the test tube from falling out of the test tube hole. The cover body is covered, and the water bath is heated for the required time of the experiment. The control switch of the hydraulic rod is adjusted. After the detection plate rises to a certain height, the handle is held and the detection plate is rotated to a certain angle. The light switch is turned on, and the gel shedding situation is observed with the assistance of the LED lamp. The latter is roughly described as including an ampoule rack, a constant temperature water bath device, a camera device and a display device electrically connected to the camera device. The ampoule rack includes a rotating rod for placing ampoules, a clamping mechanism for clamping the ampoules is arranged on the rotating rod, and the rotating rod is connected with a driving device for driving the rotating rod to rotate. When in use, after the ampoule is water-bathed for one hour, the ampoule is inverted by the detection device, and images are taken before and after the inversion.

[0004] Although the above two prior art solutions can both achieve the detection of endotoxin, in the actual detection process, there are usually multiple detections of different samples. And to ensure the reliability of the detection data, sometimes the same sample is also detected multiple times. However, the detection spaces in the above two technical solutions are relatively fixed and single, that is, once loaded, it needs to be taken out after a complete water bath reaction. Since long-term water bath insulation is required during the endotoxin detection process, the usability is poor. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the present invention provides an automated endotoxin detection device and its detection method, which can form multiple relatively independent reaction spaces, and can successively form the endotoxin detection operations of multiple samples while ensuring the basic endotoxin detection. It has a higher degree of automation, is more convenient to use, has a higher detection efficiency, and consumes less energy.

[0006] To achieve the above object, the present invention provides the following technical solution: An automated endotoxin detection device, including a base, further including a constant temperature box and a plurality of carrier boxes. An installation strip opening is provided on the base. Two covering tubes are provided in the constant temperature box. The two covering tubes are respectively communicated with a water inlet pipe and a water return pipe. A circulation pump and a heater are installed in the base. The water inlet pipe and the water return pipe are respectively communicated with the pump inlet and the pump outlet of the circulation pump. The heater is configured to form a wrapped heating with the water inlet pipe. A partition isolation structure is installed in the constant temperature box. The partition isolation structure divides the constant temperature box into a plurality of placement openings. Each of the plurality of carrier boxes includes a bottom shell and a top cover. An insertion contact seal is provided between each of the plurality of top covers and each of the plurality of bottom shells. A placement fixing frame is provided in each of the plurality of bottom shells. The plurality of bottom shells respectively match the plurality of placement openings. A pick-and-place opening is provided on the constant temperature box. A curved plate door is rotatably connected in the pick-and-place opening. A cutting follow-up structure is installed on the curved plate door. Insertion openings matching the cutting follow-up structure are provided on each of the plurality of top covers. An automatic lifting and transporting structure is provided in the constant temperature box. The automatic lifting and transporting structure is used for the movement and adjustment of the plurality of bottom shells. Two side brackets are fixedly connected to the base. The two side brackets are used for the bracket installation of the automatic lifting and transporting structure.

[0007] Preferably, the constant temperature box includes a water storage pool and a heat preservation cover. The water storage pool is fixedly connected in the installation strip opening. The heat preservation cover is fixedly connected to the base. A contact seal is provided between the heat preservation cover and the water storage pool. The two covering tubes are both provided in the water storage pool. The pick-and-place opening is provided on the heat preservation cover.

[0008] Preferably, the partition isolation structure includes a fixing strip, which is fixedly connected in the water storage tank. The fixing strip is fixedly connected with a plurality of partition plates, and all of the plurality of partition plates are fixedly connected in the water storage tank. The heights of the plurality of partition plates are all less than the depth of the water storage tank, and the tops of the plurality of partition plates are flush with the top of the water storage tank. The placing port is the area between two adjacent partition plates. A rotating isolation plate is rotatably connected between every two adjacent partition plates. A reset spring is fixedly connected to the bottom ends of the plurality of rotating isolation plates, and all of the plurality of reset springs are fixedly connected in the water storage tank.

[0009] Preferably, the cutting follow-up structure includes an external bracelet, an internal sliding frame and a rotating hook rod. The external bracelet is fixedly connected to the curved plate door. An installation cavity is formed in the curved plate door, and a track groove is formed in the installation cavity. The internal sliding frame is slidably connected in the track groove, and an elastic spring is fixedly connected in the track groove. The elastic spring is fixedly connected to the internal sliding frame. A rotating cylinder is fixedly connected to the rotating hook rod. The rotating cylinder is rotatably connected to the internal sliding frame. The rotating cylinder is located in the installation cavity. A screw-thread driving rod is slidably connected to the internal sliding frame. A through plate is arranged in the screw-thread driving rod. The through plate is fixedly connected in the rotating cylinder. A progressive driving mechanism is installed in the curved plate door, and the progressive driving mechanism is used for driving the screw-thread driving rod.

[0010] Preferably, the progressive driving mechanism includes a swinging sleeve and a track ring sleeve. The swinging sleeve is rotatably connected in the curved plate door. A telescopic strip is slidably connected in the swinging sleeve. The telescopic strip is fixedly connected with a connecting spring. The connecting spring is fixedly connected in the swinging sleeve. The telescopic strip is fixedly connected with a transmission rod. The transmission rod is inserted into the track ring sleeve. The track ring sleeve is fixedly connected to the internal sliding frame. The swinging sleeve is fixedly connected with an externally extending operating rod. A first rotating connecting piece is fixedly connected to the externally extending operating rod. The first rotating connecting piece is fixedly connected with a limiting spring. The limiting spring is fixedly connected with a second rotating connecting piece. The second rotating connecting piece is rotatably connected to the external bracelet.

[0011] Preferably, the automatic lifting and conveying structure includes a servo motor, a lead screw, and a plurality of optical bars. The lead screw is rotatably connected between the two side brackets, and the plurality of optical bars are fixedly connected between the two side brackets. The servo motor is installed on one of the two side brackets, and the output shaft of the servo motor is fixedly connected to the lead screw. A nut sleeve is threadedly connected to the lead screw, and a moving frame is fixedly connected to the outside of the nut sleeve. A plurality of optical holes are formed in the moving frame, and the plurality of optical holes respectively match the plurality of optical bars. A lifting frame is slidably connected to the moving frame. An external pressure sensor is installed on the moving frame. The top end of the lifting frame is provided with a plane matching the external pressure sensor. A magnetic driving component is installed between the lifting frame and the moving frame. The lifting frame is fixedly connected to a strip hook cylinder, and the strip hook cylinder is provided with a plurality of track members, and the plurality of track members are respectively fixedly connected to the plurality of bottom shells. Two pressure alignment components are installed on the lifting frame.

[0012] Preferably, the magnetic driving component includes a permanent magnet and a plurality of electromagnets. An installation groove is formed in the lifting frame, the permanent magnet is fixedly connected in the installation groove, and the plurality of electromagnets are all fixedly connected to the moving frame and all match the permanent magnet.

[0013] Preferably, the pressure alignment component includes two lifting hooks and two built-in pressure sensors. The two built-in pressure sensors are both installed on the lifting frame. The two lifting hooks are both slidably connected to the lifting frame. Auxiliary springs are fixedly connected to the two lifting hooks, and auxiliary pushing bars are fixedly connected to the two auxiliary springs. The two auxiliary pushing bars respectively match the two built-in pressure sensors, and the two auxiliary pushing bars are both slidably matched with the lifting frame.

[0014] Preferably, two upper gradient slopes are provided at the top end of the track member, and the two upper gradient slopes respectively match the two lifting hooks. Two lower gradient slopes are provided at the bottom end of the track member, and the two lower gradient slopes respectively match the two ends of the strip hook cylinder. A limiting surface matching the rotating isolation plate is provided on the fixed strip, and a holding ball is rotatably connected to the front end of the extended operating rod.

[0015] A detection method for an endotoxin automatic detection device: S1. Before use, install a control circuit for the endotoxin automatic detection device, and pre-install transfer warm water in the water storage tank. The circulation pump is powered on to work to suck the transfer warm water in the water storage tank out through the return pipe, and pump the sucked transfer warm water back into the water storage tank through the water inlet pipe. When the transfer warm water passes through the water inlet pipe, it will be heated by the heater to perform a temperature increase treatment on the transfer warm water to ensure that the temperature of the transfer warm water in the water storage tank is maintained; S2. During use, the operation of lifting and moving a single bottom shell among multiple bottom shells can be achieved through the operation of the automatic feeding structure, enabling the bottom shell to form a position movement adjustment between the placement port and the pick-and-place port. When it is necessary to load samples into the carrier box, the carrier box is transferred to the pick-and-place port through the operation of the automatic feeding structure. By adjusting the cutting and following structure, the top cover at the pick-and-place port forms an internal and external connection with the bent plate door. Then, during the process of opening the bent plate door, the top cover can be synchronously opened along with the opening of the bent plate door. After opening, the test tube pre-loaded with samples and horseshoe crab reagent is inserted into the placement fixing rack. S3. After the test tubes are loaded, the bent plate door is closed relative to the pick-and-place port, causing the top cover to be synchronously closed with the corresponding bottom shell. By adjusting the cutting and following structure to reset, the mutually fixed top cover and the bent plate door are separated. Thereafter, the automatic feeding structure continues to operate to control the overall carrier box formed by the bottom shell and the top cover to reset and move from the pick-and-place port to the corresponding placement port. When the bottom shell is placed into the placement port, the bottom of the bottom shell contacts the heat transfer water in the water storage tank, and under the action of heat conduction, the reaction heating of the samples and horseshoe crab reagent in the bottom shell is formed. S4. According to the above-mentioned step sequence, multiple batches of samples are respectively loaded into multiple carrier boxes. When the heat preservation reaction of the samples in the carrier box is completed, the corresponding carrier box is transferred from the placement port to the pick-and-place port again through the automatic feeding structure. Finally, the samples and horseshoe crab reagent after the reaction are taken out, and the samples and horseshoe crab reagent in the test tubes after the reaction are compared and judged to form the detection of endotoxin.

[0016] Compared with the prior art, the present invention provides an automatic endotoxin detection device and its detection method, which have the following beneficial effects: (1) In the present invention, through the design of the constant temperature box, a corresponding heat preservation space is formed in cooperation with multiple carrier boxes to facilitate the heat preservation operation of multiple samples in multiple carrier boxes. Since multiple samples can be heat-preserved simultaneously, the detection efficiency is higher. Through the design of multiple carrier boxes, multiple relatively independent reaction spaces for multiple samples are formed, enabling the endotoxin detection operations of multiple samples to be carried out successively while ensuring the basic endotoxin detection.

[0017] (2) In the present invention, through the provision of the partition isolation structure, the water storage tank is correspondingly divided into multiple placement ports. On the one hand, it is convenient for the corresponding placement of multiple bottom shells, and on the other hand, it is also convenient for the follow-up closing of the water storage tank after the bottom shell is lifted, reducing the temperature loss in the water storage tank and consuming less energy.

[0018] (3) In the present invention, through the design of the automatic feeding structure, the relative position movement adjustment between the pick-and-place port and the corresponding placement port is formed in cooperation with the carrier box to facilitate the pick-and-place operation of the samples in the carrier box, and the degree of automation is relatively high.

[0019] (4) In the present invention, through the design of the cutting-in follow-up structure, the connection adjustment between the top cover and the bent plate door at the access opening can be realized, thereby facilitating the synchronous opening of the top cover and the bent plate door and making it more convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a three-dimensional structure schematic diagram of the whole of the present invention; Figure 2 of the present invention Figure 1 is a partial enlarged structure schematic diagram at A in the present invention; Figure 3 is a three-dimensional structure schematic diagram of the cooperation of the fixing strip, the partition board and the rotating isolation board, etc. of the present invention; Figure 4 of the present invention Figure 3 is a partial enlarged structure schematic diagram at B in the present invention; Figure 5 is a three-dimensional structure schematic diagram of the partial sectional decomposition of the cooperation of the strip hook barrel, the track member and the lifting hook, etc. of the present invention; Figure 6 is a three-dimensional structure schematic diagram of another angle of the whole of the present invention; Figure 7 of the present invention Figure 6 is a partial enlarged structure schematic diagram at C in the present invention; Figure 8 is a three-dimensional structure schematic diagram of the stepped section of the cooperation of the bent plate door, the internal sliding frame and the rotating hook rod, etc. of the present invention; Figure 9 is a three-dimensional structure schematic diagram of the cooperation of the internal sliding frame, the elastic spring and the screw-thread driving rod, etc. of the present invention; Figure 10 is a three-dimensional structure schematic diagram of the partial section of the cooperation of the swinging sleeve, the track ring sleeve and the telescopic strip, etc. of the present invention; Figure 11 is a three-dimensional structure schematic diagram of the decomposition of the cooperation of the bottom shell, the top cover and the insertion fixing frame, etc. of the present invention; Figure 12 is a three-dimensional structure schematic diagram of the section of the cooperation of the water storage tank, the covering pipe and the water inlet pipe, etc. of the present invention; Figure 13 is a three-dimensional structure schematic diagram of the partial section of the rotating hook rod relative to the rotating hook state of the top cover of the present invention; Figure 14 is a three-dimensional structure schematic diagram of the stepped section of the cooperation of the bent plate door, the rotating hook rod and the extending operating rod, etc. of the present invention from the bottom view; Figure 15 is a three-dimensional structure schematic diagram of the decomposition of the cooperation of the through plate, the swinging sleeve and the track ring sleeve, etc. of the present invention; Figure 16 is a three-dimensional structure schematic diagram of the bottom view of the cooperation of the fixing strip, the partition board and the rotating isolation board, etc. of the present invention; Figure 17 This is a schematic diagram of the arrangement order of the test tubes of the present invention on the placement fixing rack.

[0021] In the figure: 1. Base; 2. Water storage tank; 3. Heat preservation cover; 4. Covering tube; 5. Water inlet pipe; 6. Water return pipe; 7. Circulation pump; 8. Heater; 9. Bottom shell; 10. Top cover; 11. Placement fixing rack; 12. Bent plate door; 13. Insertion port; 14. Side support; 15. Fixing strip; 16. Partition board; 17. Rotating isolation board; 18. Reset spring; 19. External bracelet; 20. Internal sliding rack; 21. Rotating hook rod; 22. Installation cavity; 23. Track groove; 24. Elastic spring; 25. Rotating cylinder; 26. Threaded mouth drive rod; 27. Through plate; 28. Oscillating sleeve; 29. Track ring sleeve; 30. Telescopic strip; 31. Connecting spring; 32. Transmission rod; 33. Extended operating rod; 34. First rotating connection piece; 35. Limiting spring; 36. Second rotating connection piece; 37. Servo motor; 38. Lead screw; 39. Optical bar; 40. Threaded sleeve; 41. Moving rack; 42. Lifting rack; 43. External pressure sensor; 44. Strip hook cylinder; 45. Track piece; 46. Permanent magnet; 47. Electromagnet; 48. Lifting hook; 49. Built-in pressure sensor; 50. Auxiliary spring; 51. Auxiliary pushing strip; 52. Upper gradient slope; 53. Lower gradient slope; 54. Limiting surface; 55. Holding ball. Specific embodiments

[0022] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0023] Embodiment, please refer to Figures 1 - 17, An endotoxin automatic detection device, including a base 1, also including a constant temperature box and multiple carrier boxes. There is an installation strip opening on the base 1. There are two covering tubes 4 arranged in the constant temperature box. The constant temperature box includes a water storage pool 2 and a heat preservation cover 3. The water storage pool 2 is fixedly connected in the installation strip opening, and the heat preservation cover 3 is fixedly connected to the base 1. There is a contact seal between the heat preservation cover 3 and the water storage pool 2. Both of the two covering tubes 4 are arranged in the water storage pool 2. The two covering tubes 4 are respectively communicated with a water inlet pipe 5 and a water return pipe 6. A circulation pump 7 and a heater 8 are installed in the base 1. The water inlet pipe 5 and the water return pipe 6 are respectively communicated with the pump inlet and the pump outlet of the circulation pump 7, which can realize the circulating drive of the heat transfer water in the constant temperature box. The heater 8 is matched with the water inlet pipe 5 to form a wrapped heating. Through the design of the constant temperature box, multiple carrier boxes are matched to form a corresponding water bath heat preservation space to facilitate the water bath heat preservation operation of multiple samples in the multiple carrier boxes. Since multiple samples can carry out the heat preservation operation synchronously, the detection efficiency is higher. A partition isolation structure is installed in the constant temperature box. The partition isolation structure divides the constant temperature box into multiple placement openings to provide a necessary placement space for multiple bottom shells 9. The partition isolation structure includes a fixed strip 15. The fixed strip 15 is fixedly connected in the water storage pool 2. The fixed strip 15 is fixedly connected with multiple partition plates 16. Multiple partition plates 16 are all fixedly connected in the water storage pool 2. The height of multiple partition plates 16 is less than the depth of the water storage pool 2. The tops of multiple partition plates 16 are flush with the top of the water storage pool 2, so that there is a space through which the heat transfer water can flow between the bottom end of the partition plate 16 and the inner bottom wall of the water storage pool 2. The placement opening is the area between two adjacent partition plates 16. A rotating isolation plate 17 is rotatably connected between every two adjacent partition plates 16. The bottom ends of multiple rotating isolation plates 17 are all fixedly connected with a return spring 18. Multiple return springs 18 are all fixedly connected in the water storage pool 2. Through the configuration of the partition isolation structure, the water storage pool 2 is correspondingly divided into multiple placement openings. On the one hand, it is convenient to match the corresponding placement of multiple bottom shells 9. On the other hand, it is also convenient for the water storage pool 2 to be closed following the lifting of the bottom shell 9, reducing the temperature loss in the water storage pool 2 and consuming less energy. The fixed strip 15 is provided with a limiting surface 54 matching the rotating isolation plate 17. When the bottom shell 9 is lifted out of the water storage pool 2, the downward pressure acting on the rotating isolation plate 17 fails. Under the elastic reset action of the return spring 18, the rotating isolation plate 17 rotates and rises in the water storage pool 2. The limiting surface 54 forms a limit for the rotating isolation plate 17 after it rotates and rises to ensure that the rotating isolation plate 17 just covers and seals the placement opening where it is located after rotating and rising. Multiple carrier boxes all include a bottom shell 9 and a top cover 10. There is an insertion contact seal between multiple top covers 10 and multiple bottom shells 9 respectively. Multiple placement fixing frames 11 are arranged in multiple bottom shells 9. Through the design of multiple carrier boxes, multiple relatively independent reaction spaces for multiple samples are formed, and the endotoxin detection operations of multiple samples can be carried out successively while ensuring the basic endotoxin detection. Multiple bottom shells 9 respectively match multiple placement openings.

[0024] It should be further noted that an access opening is provided on the incubator. The access opening is provided on the heat preservation cover 3. A bent plate door 12 is rotatably connected inside the access opening, which can form a necessary closure for the access opening. An insertion following structure is installed on the bent plate door 12. Insertion openings 13 matching the insertion following structure are provided on multiple top covers 10. The insertion following structure includes an external hand ring 19, an internal sliding frame 20, and a rotating hook rod 21. The external hand ring 19 is fixedly connected to the bent plate door 12. An installation cavity 22 is provided on the bent plate door 12. A track groove 23 is provided inside the installation cavity 22. The internal sliding frame 20 is slidably connected in the track groove 23, and an elastic spring 24 is fixedly connected inside the track groove 23. The elastic spring 24 is fixedly connected to the internal sliding frame 20. A rotating cylinder 25 is fixedly connected to the rotating hook rod 21. The rotating cylinder 25 is rotatably connected to the internal sliding frame 20. The rotating cylinder 25 is located inside the installation cavity 22. A screw-thread driving rod 26 is slidably connected to the internal sliding frame 20. A through plate 27 is arranged inside the screw-thread driving rod 26. The through plate 27 is fixedly connected inside the rotating cylinder 25. A progressive driving mechanism is installed inside the bent plate door 12. The progressive driving mechanism is used to drive the screw-thread driving rod 26. Through the design of the insertion following structure, the connection adjustment between the top cover 10 entering the access opening and the bent plate door 12 can be realized, and then the synchronous opening of the top cover 10 and the bent plate door 12 is facilitated, making the use more convenient. The progressive driving mechanism includes a swinging sleeve 28 and a track ring sleeve 29. The swinging sleeve 28 is rotatably connected inside the bent plate door 12. A telescopic strip 30 is slidably connected inside the swinging sleeve 28. A connecting spring 31 is fixedly connected to the telescopic strip 30. The connecting spring 31 is fixedly connected inside the swinging sleeve 28. The telescopic strip 30 is fixedly connected to a transmission rod 32. The transmission rod 32 is inserted into the track ring sleeve 29. The track ring sleeve 29 is fixedly connected to the internal sliding frame 20. The swinging sleeve 28 is fixedly connected to an externally extending operating rod 33. A first rotating connecting piece 34 is fixedly connected to the externally extending operating rod 33. A limiting spring 35 is fixedly connected to the first rotating connecting piece 34. The limiting spring 35 is fixedly connected to a second rotating connecting piece 36. The second rotating connecting piece 36 is rotatably connected to the external hand ring 19, realizing the auxiliary limit after the rotation and elevation of the rotating hook rod 21, and can also control the auxiliary limit after the lowering and turning down of the rotating hook rod 21. A holding ball 55 is rotatably connected to the front end of the externally extending operating rod 33, facilitating the auxiliary grasping when applying force to the externally extending operating rod 33.

[0025] It should be further noted that an automatic lifting and conveying structure is provided inside the incubator. The automatic lifting and conveying structure is used for the movement and adjustment of multiple bottom shells 9. Two side brackets 14 are fixedly connected to the base 1, and the two side brackets 14 are used for the bracket installation of the automatic lifting and conveying structure. The automatic lifting and conveying structure includes a servo motor 37, a lead screw 38, and multiple optical bars 39. The lead screw 38 is rotatably connected between the two side brackets 14, and the multiple optical bars 39 are all fixedly connected between the two side brackets 14. Through holes corresponding to the lead screw 38 and the multiple optical bars 39 are provided on the heat preservation cover 3. The servo motor 37 is installed on one of the two side brackets 14, and the output shaft of the servo motor 37 is fixedly connected to the lead screw 38. A nut sleeve 40 is threadedly connected to the lead screw 38. A moving frame 41 is fixedly connected to the outside of the nut sleeve 40. Multiple light holes are provided on the moving frame 41, and the multiple light holes respectively match the multiple optical bars 39. A lifting frame 42 is slidably connected to the moving frame 41. An external pressure sensor 43 is installed on the moving frame 41. A plane matching the external pressure sensor 43 is provided at the top of the lifting frame 42. A magnetic drive assembly is installed between the lifting frame 42 and the moving frame 41. The lifting frame 42 is fixedly connected to a strip hook cylinder 44. Multiple track members 45 are provided for the strip hook cylinder 44, and the multiple track members 45 are respectively fixedly connected to the multiple bottom shells 9. Two pressure alignment components are installed on the lifting frame 42. The magnetic drive assembly includes a permanent magnet 46 and multiple electromagnets 47. An installation groove is provided on the lifting frame 42, and the permanent magnet 46 is fixedly connected in the installation groove. The multiple electromagnets 47 are all fixedly connected to the moving frame 41, and the multiple electromagnets 47 all match the permanent magnet 46. The pressure alignment components include two lifting hooks 48 and two internal pressure sensors 49. The two internal pressure sensors 49 are both installed on the lifting frame 42. The two lifting hooks 48 are both slidably connected to the lifting frame 42. Auxiliary springs 50 are fixedly connected to the two lifting hooks 48. Auxiliary push bars 51 are fixedly connected to the two auxiliary springs 50, and the two auxiliary push bars 51 respectively match the two internal pressure sensors 49. The two auxiliary push bars 51 are both slidably matched with the lifting frame 42. Two upper gradient slopes 52 are provided at the top of the track member 45, and the two upper gradient slopes 52 respectively match the two lifting hooks 48. Two lower gradient slopes 53 are provided at the bottom of the track member 45, and the two lower gradient slopes 53 respectively match the two ends of the strip hook cylinder 44. Through the design of the automatic lifting and conveying structure, the relative position between the self-taking and placing opening and the corresponding placing opening of the supporting carrier box is adjusted, so as to facilitate the taking and placing operations of the samples in the carrier box, and the degree of automation is relatively high.

[0026] The servo motor 37, electromagnetic body 47, circulation pump 7, heater 8, external pressure sensor 43, and internal pressure sensor 49 in this embodiment are all conventional devices that are commercially available and well-known to those skilled in the art. In the present invention, we only use them and do not improve their structures and functions. For those skilled in the art, their setting methods, installation methods, and electrical connection methods can be debugged and operated as long as the requirements of their user manuals are followed, and thus will not be elaborated herein.

[0027] In summary, the working principle of the endotoxin automatic detection device and its detection method is as follows. Before use, a control circuit is installed for the endotoxin automatic detection device, and circulating water is pre-loaded in the water storage tank 2. The circulating pump 7 is powered on to suck the circulating water in the water storage tank 2 out through the return pipe 6, and then pump the sucked circulating water back into the water storage tank 2 through the inlet pipe 5. When the circulating water passes through the inlet pipe 5, it will be heated by the heater 8 to raise the temperature of the circulating water, so as to ensure the temperature of the circulating water in the water storage tank 2. During use, through the operation of the automatic lifting and conveying structure, the lifting and moving operations of a single bottom shell 9 among multiple bottom shells 9 can be realized, so that the bottom shell 9 can be adjusted in position between the placement port and the access port. When it is necessary to load samples into the carrier box, the carrier box is transferred to the access port through the operation of the automatic lifting and conveying structure. By adjusting the cutting-in follow-up structure, the top cover 10 at the access port is connected to the bent plate door 12 inside and outside. Then, when the bent plate door 12 is opened, the top cover 10 can be synchronously opened along with the opening of the bent plate door 12. After opening, the test tubes pre-loaded with samples and horseshoe crab reagents are inserted into the placement fixing frame 11. And for the convenience of synchronously loading multiple test tubes, the placement fixing frame 11 and the bottom shell 9 adopt a structure that can be quickly disassembled. Multiple test tubes are pre-inserted into the placement fixing frame 11 outside, and by installing the placement fixing frame 11 relative to the bottom shell 9, multiple test tubes can be quickly loaded into the bottom shell 9 synchronously. After the test tubes are loaded, the bent plate door 12 is closed relative to the access port, so that the top cover 10 and the corresponding bottom shell 9 are synchronously closed, and by resetting the cutting-in follow-up structure, the mutually fixed top cover 10 and bent plate door 12 are separated. Thereafter, the automatic lifting and conveying structure continues to work to control the overall carrier box formed by the bottom shell 9 and the top cover 10 to reset and move from the access port to the corresponding placement port. When the bottom shell 9 is placed in the placement port, the bottom of the bottom shell 9 contacts the circulating water in the water storage tank 2, and the samples and horseshoe crab reagents in the bottom shell 9 are heated by heat conduction to react. According to the above steps, multiple batches of samples are respectively loaded into multiple carrier boxes. When the samples in the carrier box complete the heat preservation reaction, the corresponding carrier box is transferred from the placement port to the access port again through the automatic lifting and conveying structure, and finally the samples and horseshoe crab reagents after the reaction are taken out, and the samples and horseshoe crab reagents in the test tubes after the reaction are compared and judged to form the detection of endotoxin. For the convenience of observing the reaction state of the test tubes in the carrier box and recording the reaction process, the heat preservation cover 3 and multiple top covers 10 are made of transparent materials, and multiple cameras are installed in the heat preservation cover 3 to form video records of the interiors of multiple carrier boxes.

[0028] Further, the operating principle of the automatic feeding structure is as follows: when the servo motor 37 is powered on and operates, it realizes the rotational adjustment of the lead screw 38 relative to the two side brackets 14. Under the cooperation of the lead screw 38 and the nut sleeve 40 and the guiding effect of the optical bar 39 on the moving frame 41, the rotating lead screw 38 can realize the position movement of the moving frame 41 along the optical bar 39. Since the lifting frame 42 is slidably connected to the moving frame 41 in the vertical direction, the lifting frame 42 will move synchronously with the position movement of the moving frame 41 along the optical bar 39. When none of the multiple electromagnets 47 is energized to generate an electromagnetic field, under the action of the self-gravity of the carrier box, the carrier box falls into the corresponding placement opening. Since the rail member 45 is fixedly connected to the bottom case 9, the rail member 45 will also fall when the bottom case 9 falls into the placement opening. After multiple bottom cases 9 respectively fall into multiple placement openings, multiple rail members 45 will also be at the same height with similar heights. Due to the self-gravity of the lifting frame 42, it will also fall relative to the moving frame 41. And since multiple rail members 45 are all slidably engaged with the strip hook cylinder 44, in the state where none of the multiple electromagnets 47 generates an electromagnetic field, the strip hook cylinder 44 can form a sliding switching engagement with multiple rail members 45. The two lifting hooks 48 are pre-adjusted to enter the upper gradient slopes 52 at both ends of the same rail member 45 respectively. By comparing and observing the readings of the two built-in pressure sensors 49, due to the action of the two auxiliary springs 50, the two lifting hooks 48 will respectively form contact fits with the two upper gradient slopes 52, maintaining the contact fit state of the two lifting hooks 48 with the upper gradient slopes 52 at both ends of the same rail member 45 respectively, and finely adjusting the relative positions of the two lifting hooks 48 relative to the rail member 45 until the two built-in pressure sensors 49 have similar readings within a certain range, and this range value is set. After that, as long as both of the two built-in pressure sensors 49 enter this range value, the positions of the two lifting hooks 48 can be judged. In this position, the strip hook cylinder 44 completely covers the single rail member 45 inside it and does not form a connection with other rail members 45. In this state, the multiple electromagnets 47 are correspondingly energized. Under the relative cooperation of the electromagnets 47 and the permanent magnet 46, the relative position of the lifting frame 42 relative to the moving frame 41 can be increased. To ensure the lifting effect of the lifting frame 42, the N pole and S pole of the permanent magnet 46 are arranged vertically opposite to each other. The multiple electromagnets 47 are energized in sequence from bottom to top to form an electromagnetic field acting on the permanent magnet 46, controlling the magnetic pole direction of the electromagnets 47, so that the electromagnets 47 above the permanent magnet 46 generate an attractive force on the permanent magnet 46, and the electromagnets 47 below the permanent magnet 46 generate a repulsive force on the permanent magnet 46, so that the lifting frame 42 has an upward pulling and downward pushing force to ensure the lifting of the lifting frame 42 relative to the moving frame 41. When the plane on the lifting frame 42 contacts the external pressure sensor 43 and makes the external pressure sensor 43 have a pressure reading, it means that the lifting frame 42 has been lifted in place. In this state, the bottom case 9 connected to the rail member 45 located in the strip hook cylinder 44 is lifted synchronously.Moreover, there is a safety distance in height between the bottom end of the lifted bottom case 9 and the other top covers 10 that are not lifted. After that, when the servo motor 37 operates, the lifted bottom case 9 can be moved within the heat preservation cover 3, facilitating the adjustment of the entry of the lifted bottom case 9 relative to the pick-and-place opening.

[0029] Furthermore, when the bottom case 9 enters the pick-and-place opening, the top cover 10 on the bottom case 9 also enters the pick-and-place opening. By controlling the number of turns of the servo motor 37 during rotation, the complete alignment of the bottom case 9 relative to the pick-and-place opening is achieved. After that, the servo motor 37 is controlled to stop, as shown in the appendix Figure 2As shown, under normal conditions, the swinging sleeve 28 is in a relatively elevated state relative to the exposed end of the bent plate door 12. In this state, the limiting spring 35 can limit the swinging sleeve 28. In this state, the screw-thread driving rod 26 is in a certain relative pulled-out state relative to the rotating cylinder 25, and the rotating hook rod 21 rotates and falls completely into the installation cavity 22. On the basis of the above state, the operator holds the holding ball 55 with the palm of the hand and applies force to the extending operating rod 33 through the holding ball 55, so that the extending operating rod 33 forms a rotational adjustment relative to the bent plate door 12 to control the rotating and rising of the telescopic strip 30. Due to the action of the connecting spring 31 and the elastic spring 24, at the initial stage of the rotating and rising of the telescopic strip 30, the sliding drive of the screw-thread driving rod 26 relative to the internal carriage 20 will be realized, that is, the screw-thread driving rod 26 forms a sliding insertion relative to the rotating cylinder 25. Under the transmission action of the through plate 27 and the screw-thread driving rod 26, during the insertion process of the screw-thread driving rod 26 relative to the rotating cylinder 25, the rotating cylinder 25 will form a rotation relative to the internal carriage 20, and finally achieve the rising drive of the rotating hook rod 21. The rotating and rising rotating hook rod 21 will form an insertion relative to the insertion port 13. And when the rotating hook rod 21 rotates to the limit position of the insertion port 13, the rotating hook rod 21 will not rotate and rise further, but further rotate and press down the swinging sleeve 28. Under the transmission action of the telescopic strip 30, the transmission rod 32 will be further elevated. Since the track ring sleeve 29 is fixedly connected to the screw-thread driving rod 26 and the screw-thread driving rod 26 cannot further move relative to the internal carriage 20, the track ring sleeve 29 cannot further approach the internal carriage 20. Thereafter, the elevated transmission rod 32 will drive the internal carriage 20 to rise along the track groove 23, and at the same time, the telescopic strip 30 will also overcome the elastic force of the connecting spring 31 and form a further insertion relative to the swinging sleeve 28. The rising of the internal carriage 20 will drive the rotating hook rod 21 to rise through the rotating cylinder 25. The rising rotating hook rod 21 will drive the top cover 10 hooked and connected by it to rise synchronously until the top cover 10 forms mutual fitting and pressing limit with the inner top wall of the bent plate door 12. After that, the top cover 10 cannot rise further, and the rotating hook rod 21 cannot rise further. During this process, the rising top cover 10 will form a relative pull-out relative to the bottom shell 9 originally inserted therein to avoid mutual interference with the originally inserted bottom shell 9 during the subsequent synchronous opening process of the top cover 10 and the bent plate door 12. The rotation angle of the swinging sleeve 28 in this state will cause the limiting spring 35 to enter the elongation state again after passing through the extreme compression state from the elongation state. The limiting spring 35 in this state can form auxiliary limit after the rising of the rotating and rising telescopic strip 30 to avoid the connection limit between the rotating hook rod 21 and the top cover 10 from failing. In this state, by applying a rotational opening force to the bent plate door 12 through the external hand ring 19, the opening of the bent plate door 12 can be realized, and the top cover 10 fixedly limited inside the bent plate door 12 will also form synchronous opening.

[0030] Continuing further, after the operation is completed, first close the curved plate door 12 and then lift the extended operating rod 33 upwards, then the top cover 10 can be inserted and closed relative to the bottom shell 9. Due to the elastic reset function of the elastic spring 24, the top cover 10 will first form a relative downward insertion relative to the bottom shell 9, and then the rotating hook rod 21 will rotate out and escape relative to the insertion port 13 on the top cover 10. And during this process, the limit spring 35 will pass through the extreme compression state again and enter the elongation state, so that the limit after the telescopic strip 30 rotates and lowers, that is, the limit when the rotating hook rod 21 is retracted into the installation cavity 22. After that, the servo motor 37 runs to realize the reset of the base 1 relative to the original corresponding placement port. Finally, the multiple electromagnets 47 are powered off in sequence from top to bottom, and the lifting frame 42 is reset to fall relative to the moving frame 41 to ensure that the bottom shell 9 falls smoothly into the placement port. After the bottom shell 9 falls into the placement port, it will drive the rotating isolation plate 17 to form a downward rotation drive, so the bottom end of the bottom shell 9 will contact the heat transfer water in the water storage tank 2 to ensure effective heat conduction. The covering pipe 4 connected to the water inlet pipe 5 is located on the side where the rotating isolation plate 17 falls. The heat transfer water entering the water storage tank 2 through the covering pipe 4 will be guided by the inclined rotating isolation plate 17 to facilitate the better contact between the heated heat transfer water and the bottom shell 9. And considering the influence of the water path lengths of the middle part and both ends of the covering pipe 4, the diameter of the flow holes on the covering pipe 4 adopts a structure that gradually decreases from both ends to the middle. When it is necessary to perform an oscillation operation on the test tube, by controlling one or two of the multiple electromagnets 47 located at the bottommost side to be repeatedly powered on and off, the reciprocating rise and fall of the test tube can be realized, so as to achieve the purpose of test tube oscillation.

[0031] The preparation of the samples and the horseshoe crab reagent in the sample and horseshoe crab reagent test tubes is based on the following: λ is the labeled sensitivity (EU / ml) of the horseshoe crab reagent in the gel method, which is a known number. Due to different usage purposes, manufacturers have multiple specifications of labeled sensitivities, such as: 0.03 EU / ml, 0.06 EU / ml, 0.125 EU / ml, 0.25 EU / ml, 0.5 EU / ml, 1.0 EU / ml, 2.0 EU / ml.

[0032] During the use of λ, it is necessary to be based on the labeled sensitivity of the actually purchased horseshoe crab reagent.

[0033] The specific value of the labeled sensitivity is not specified here, and λ is used to replace (a sensitivity symbol commonly recognized in the industry) the labeled sensitivity. It can be directly regarded as a known number without affecting the calculation.

[0034] Reagent preparation (using the endotoxin working standard to prepare)

[0035] Experimental steps Preparation of reaction tubes (the arrangement order of the tubes on the fixing rack 11 is as shown in the appendix Figure 17 as shown)

[0036] Dissolution of Limulus reagent: Add water for bacterial endotoxin test to the Limulus reagent according to the labeled amount (the specification of the purchased reagent), gently shake to completely dissolve the Limulus reagent, pay attention not to cause bubbles, and the dissolved reagent should be used within 10 minutes (i.e., prepared and used immediately).

[0037] It is necessary that 0.1 ml / tube of Limulus reagent participates in the reaction (corresponding to the red marking in the table). If the amount is excessive, it should have the function of being dispensed into 0.1 ml / EP tubes.

[0038] Reaction on the instrument Seal the tube mouth, place it in the circulating water at 37 °C in the water storage tank and incubate for 60 minutes ± 2 minutes. The tube should be shaken at the initial stage when the base returns to the inlet position to facilitate the mixing of the sample and Limulus reagent in the tube, and vibration should be avoided during the subsequent incubation period.

[0039] Result determination and result observation are as follows: Gently take out the tube from the fixing rack 11, slowly invert it 180°. If the content after the reaction of the sample and Limulus reagent in the tube shows a firm gel, does not deform, and does not slip off the tube wall, it is positive, recorded as (+); If it does not form a gel or although a gel is formed but cannot remain intact and slips off the tube wall, it is negative, recorded as (-).

[0040] Result interpretation 1. Only when the results of all negative control tubes are negative, and the results of positive control tubes and test article positive control tubes are all positive, the test is valid, otherwise it is invalid.

[0041] 2. If the negative control tube is positive, it indicates that the Limulus reagent, water for bacterial endotoxin test, or experimental utensils may be contaminated. If the positive control tube is negative, it indicates that the activity of the Limulus reagent is lost, the titer of the endotoxin standard solution is reduced, the sensitivity of the Limulus reagent or the titer indication of the endotoxin is inaccurate, or the dilution of the endotoxin standard solution is incorrect.

[0042] 3. If the test article positive control tube is negative, it indicates that there are interfering factors in the reaction system.

[0043] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An automated endotoxin detection device, comprising a base (1), characterized in that: It also includes a thermostatic box and a plurality of carrier boxes. The base (1) is provided with a mounting strip. Two covering pipes (4) are arranged in the thermostatic box. The two covering pipes (4) are respectively connected to a water inlet pipe (5) and a water return pipe (6). A circulating pump (7) and a heater (8) are installed in the base (1). The water inlet pipe (5) and the water return pipe (6) are respectively connected to a pump inlet and a pump outlet of the circulating pump (7). The heater (8) is matched with the water inlet pipe (5) to form a covering heating. A partition isolation structure is installed in the thermostatic box. The partition isolation structure divides the thermostatic box into a plurality of inlets. The plurality of carrier boxes each include a bottom shell (9) and a top cover (10). The plurality of top covers (10) are respectively Insertion contact seals are provided between the multiple bottom shells (9), insertion fixing frames (11) are provided in the multiple bottom shells (9), the multiple bottom shells (9) are matched with the multiple insertion ports respectively, a take-in and put-out port is provided on the thermostatic box, a curved plate door (12) is rotatably connected in the take-in and put-out port, a cut-in follow-up structure is installed on the curved plate door (12), insertion ports (13) matching the cut-in follow-up structure are provided on the multiple top covers (10), an automatic lifting structure is provided in the thermostatic box, the automatic lifting structure is used for moving and adjusting the multiple bottom shells (9), two side brackets (14) are fixedly connected to the base (1), and the two side brackets (14) are used for bracket installation of the automatic lifting structure.

2. An automated endotoxin detection device according to claim 1, characterized in that: The thermostatic box comprises a water tank (2) and a heat-insulating cover (3); the water tank (2) is fixedly connected in the mounting strip opening; the heat-insulating cover (3) is fixedly connected to the base (1); a contact seal is provided between the heat-insulating cover (3) and the water tank (2); the two covering pipes (4) are both provided in the water tank (2); and the access opening is provided on the heat-insulating cover (3).

3. An automated endotoxin detection device according to claim 2, characterized in that: The partition isolation structure comprises a fixing bar (15), the fixing bar (15) being fixedly connected in the water tank (2), the fixing bar (15) being fixedly connected to a plurality of partition plates (16), the plurality of partition plates (16) being fixedly connected in the water tank (2), the height of the plurality of partition plates (16) being less than the depth of the water tank (2), the top ends of the plurality of partition plates (16) being flush with the top end of the water tank (2), the insertion port being an area between two adjacent partition plates (16), a rotating isolation plate (17) being rotatably connected between each two adjacent partition plates (16), the bottom ends of the plurality of rotating isolation plates (17) being fixedly connected to a return spring (18), the plurality of return springs (18) being fixedly connected in the water tank (2).

4. An automated endotoxin detection device according to claim 3, characterized in that: The cut-in follow-up structure comprises an external hand ring (19), an internal slide (20) and a rotating hook rod (21); the external hand ring (19) is fixedly connected to the curved plate door (12); a mounting cavity (22) is provided on the curved plate door (12); a track groove (23) is provided in the mounting cavity (22); the internal slide (20) is slidably connected in the track groove (23); an elastic spring (24) is fixedly connected in the track groove (23); the elastic spring (24) is fixedly connected to the internal slide (20); the rotating hook rod (21) A rotating cylinder (25) is fixedly connected to the movable hook rod (21), and the rotating cylinder (25) is rotatably connected to the internal slide (20). The rotating cylinder (25) is located in the installation cavity (22), and the internal slide (20) is slidably connected to a screw-type driving rod (26). A through plate (27) is provided in the screw-type driving rod (26), and the through plate (27) is fixedly connected in the rotating cylinder (25). A progressive driving mechanism is installed in the curved plate door (12), and the progressive driving mechanism is used to drive the screw-type driving rod (26).

5. An automated endotoxin detection device according to claim 4, characterized in that: The progressive drive mechanism comprises a swing sleeve (28) and a track ring sleeve (29), the swing sleeve (28) being rotatably connected in the curved plate door (12), a telescopic bar (30) being slidably connected in the swing sleeve (28), the telescopic bar (30) being fixedly connected to a connecting spring (31), the connecting spring (31) being fixedly connected in the swing sleeve (28), the telescopic bar (30) being fixedly connected to a transmission rod (32), the transmission rod (32) being inserted into the track ring sleeve (29), the track ring sleeve (29) being fixedly connected to the internal slide (20), the swing sleeve (28) being fixedly connected to an outwardly extending operating rod (33), the outwardly extending operating rod (33) being fixedly connected to a first transfer member (34), the first transfer member (34) being fixedly connected to a limit spring (35), the limit spring (35) being fixedly connected to a second transfer member (36), the second transfer member (36) being rotatably connected to the external hand ring (19).

6. An automated endotoxin detection device according to claim 5, characterized in that: The automatic lifting structure comprises a servo motor (37), a lead screw (38) and a plurality of optical rods (39); the lead screw (38) is rotatably connected between the two side brackets (14); the plurality of optical rods (39) are fixedly connected between the two side brackets (14); the servo motor (37) is mounted on one of the two side brackets (14); the output shaft of the servo motor (37) is fixedly connected to the lead screw (38); a thread sleeve (40) is threadedly connected to the lead screw (38); a movable frame (41) is fixedly connected to the outside of the thread sleeve (40); and a plurality of optical holes are opened on the movable frame (41); The plurality of light holes are matched with the plurality of light bars (39) respectively; the movable frame (41) is slidably connected with the lifting frame (42); the movable frame (41) is equipped with an external pressure sensor (43); a plane matching the external pressure sensor (43) is arranged at the top of the lifting frame (42); a magnetic drive component is installed between the lifting frame (42) and the movable frame (41); the lifting frame (42) is fixedly connected with a hook cylinder (44); the hook cylinder (44) is equipped with a plurality of track components (45); the plurality of track components (45) are fixedly connected with the plurality of bottom shells (9) respectively; and two pressure alignment components are installed on the lifting frame (42).

7. An automated endotoxin detection device according to claim 6, characterized in that: The magnetic drive assembly comprises a permanent magnet (46) and a plurality of electromagnets (47); a mounting groove is provided on the lifting frame (42); the permanent magnet (46) is fixedly connected in the mounting groove; the plurality of electromagnets (47) are all fixedly connected to the moving frame (41); and the plurality of electromagnets (47) are all matched with the permanent magnet (46).

8. An automated endotoxin detection device according to claim 7, characterized in that: The pressure alignment component comprises two lifting hooks (48) and two built-in pressure sensors (49). The two built-in pressure sensors (49) are both mounted on the lifting frame (42). The two lifting hooks (48) are both slidably connected to the lifting frame (42). The two lifting hooks (48) are both fixedly connected with auxiliary springs (50). The two auxiliary springs (50) are both fixedly connected with auxiliary push bars (51). The two auxiliary push bars (51) are matched with the two built-in pressure sensors (49) respectively. The two auxiliary push bars (51) are both slidably matched with the lifting frame (42).

9. An automated endotoxin detection device according to claim 8, characterized in that: The top end of the track member (45) is provided with two upper gradient slopes (52), which are matched with the two lifting hooks (48) respectively; the bottom end of the track member (45) is provided with two lower gradient slopes (53), which are matched with the two ends of the hook tube (44) respectively; the fixed bar (15) is provided with a limit surface (54) which is matched with the rotating isolation plate (17); and the front end of the extended operating rod (33) is rotatably connected with a holding ball (55).

10. A detection method of an automated endotoxin detection device, characterized in that: An automated endotoxin detection device according to any one of claims 1 to 9 is used, comprising the following steps: S1. Before use, a control circuit is installed for the automatic endotoxin detection device, and warm water is pre-installed in the water storage tank (2). The circulating pump (7) is powered on to suck the warm water out of the water storage tank (2) through the return pipe (6), and the sucked warm water is pumped back into the water storage tank (2) through the water inlet pipe (5). When the warm water passes through the water inlet pipe (5), it is heated by the heater (8) to form a temperature-raising treatment of the warm water, so as to ensure that the temperature of the warm water in the water storage tank (2) is maintained; S2. During use, the automatic lifting structure can be used to lift and move a single bottom shell (9) among the multiple bottom shells (9), so that the bottom shell (9) can be adjusted to move between the insertion port and the access port. When it is necessary to load a sample into the carrier box, the automatic lifting structure is used to transfer the carrier box to the access port. The cut-in follower structure is adjusted to form an internal and external connection between the top cover (10) located at the access port and the curved plate door (12). Then, in the process of opening the curved plate door (12), the top cover (10) can be opened synchronously with the opening of the curved plate door (12). After opening, the test tube pre-loaded with the sample and horseshoe crab reagent is inserted into the fixed frame (11); S3, after the test tube is loaded, the bent plate door (12) is closed relative to the take-in and put-out opening, so that the top cover (10) and the corresponding bottom shell (9) are synchronously closed, and the top cover (10) and the bent plate door (12) fixed to each other are separated by adjusting the cut-in follower structure to reset, and then the automatic lifting structure continues to work to control the bottom shell (9) and the top cover (10) to reset the carrier box as a whole from the take-in and put-out opening to the corresponding insertion opening. When the bottom shell (9) is placed in the insertion opening, the bottom of the bottom shell (9) contacts the temperature transfer water in the water storage tank (2), and the reaction heating of the sample and the horseshoe crab reagent in the bottom shell (9) is formed under the action of heat conduction; S4. Complete the loading of multiple batches of samples into multiple carrier boxes in sequence according to the aforementioned steps. When the sample insulation reaction in the carrier box is completed, the corresponding carrier box is transferred from the inlet to the outlet again through the automatic lifting structure, and finally the samples and horseshoe crab reagents after the reaction are taken out, and the samples and horseshoe crab reagents after the reaction in the test tube are compared and judged to form an endotoxin detection.

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