A long-lasting deodorizing cat litter composition, its preparation method and application

Abstract

This invention relates to the field of pet cat litter technology, specifically to a long-lasting deodorizing cat litter composition, its preparation method, and its application. The composition comprises probiotics (Lactobacillus rhamnosus (ATCC53103) and Lactobacillus salivarius (CICC23174), 1-5%), a complex enzyme preparation (cellulase + protease + chitinase, 0.5-2%), polypeptides (EQKMFQIVGG), diatomaceous earth carrier (85-95%), 0.1-0.5% lemongrass essential oil, and 0.5-2% trehalose. This composition inhibits pathogenic bacteria such as Escherichia coli and Staphylococcus aureus through the secretion of bacteriocins by the probiotics, and the complex enzymes directionally decompose odor-causing substances such as hydrogen sulfide and ammonia. This long-lasting deodorizing cat litter composition solves the problems of poor safety, limited functionality, and insufficient long-lasting effect of traditional cat litter chemical additives, and is suitable for the field of pet excrement treatment.

Description

Technical Field

[0001] This invention relates to the field of pet cat litter technology, and more specifically, to a long-lasting deodorizing cat litter composition, its preparation method, and its application. Background Art

[0002] With the increasing popularity of pet cat ownership, cat litter, as a core product for handling pet excrement, faces increasingly stringent performance requirements. Currently, most cat litters on the market rely on chemical deodorizers (such as nano-silver and tea polyphenols) or single antibacterial ingredients (such as bamboo fiber flavonoid extract) to achieve antibacterial and deodorizing effects. However, these methods have significant limitations:

[0003] 1. Safety issues of chemical additives: Although heavy metal compounds such as silver titanium powder have significant antibacterial effects (≥99.999%), they are expensive and may pose potential risks to pets' health; traditional antifungal agents require higher doses because the natural nutrients in plant fibers can easily accelerate the growth of microorganisms, which may cause respiratory irritation.

[0004] 2. Functional limitations: Plant fiber cat litter (such as tofu cat litter and bentonite cat litter) relies on physical adsorption and natural fragrance for deodorization, and is not efficient enough in decomposing odorous substances such as hydrogen sulfide and methanethiol unique to cat feces (deodorization rate is only about 80%); bamboo fiber substrate has a narrow antibacterial spectrum and has no repellent effect on parasites such as Demodex mites.

[0005] 3. Insufficient long-term effectiveness: Cat litter can be stored in the litter box for up to 14 days, and chemical antibacterial agents are prone to failure due to changes in humidity and temperature, leading to secondary pollution.

[0006] To address the above issues, existing technologies attempt to improve performance through composite formulations. For example:

[0007] The prior art CN111149710A discloses an odor-removing and antibacterial diatomaceous earth cat litter, which achieves antibacterial effect by loading silver ions and odor removal effect by modifying diatomaceous earth; however, silver ion bactericides have small particle size and strong penetrability, which may have acute toxic effects on pets, and the raw material cost is high.

[0008] The existing technology CN112841050A uses bamboo fiber flavonoid extract as a natural antibacterial agent, achieving a sterilization rate of 97%-98% against common bacteria such as Escherichia coli, but it does not involve mite repellency and is relatively expensive.

[0009] The existing technology CN119096891A innovatively introduces nano zinc chloride combined with probiotics to decompose odor substances through enzymatic hydrolysis, but it does not solve the potential toxicity problem of nanomaterials.

[0010] Furthermore, industry technology trends indicate that biotechnology and artificial intelligence are becoming key areas for breakthroughs:

[0011] (1) Bioenzyme technology: such as cellulase and protease can decompose fecal organic matter in a targeted manner and reduce ammonia release;

[0012] (2) Synergistic effect of probiotics: Lactobacillus rhamnosus (ATCC53103) and other strains inhibit the growth of pathogens by secreting bacteriocins, while also being tolerant of acidic environments;

[0013] (3) Application of environmentally friendly materials: Porous silica gel air purification particles (CN104258806A) have a removal rate of over 96% for odorous substances such as ammonia, and require no additional preservatives.

[0014] However, existing compound formulations still face stability challenges: probiotics are easily inactivated during high-temperature granulation, and enzyme preparations are easily adsorbed by carrier materials, reducing their activity. Therefore, developing a cat litter composition that combines long-lasting deodorization, broad-spectrum antibacterial properties, and mite repellency remains a technological bottleneck that the industry urgently needs to overcome. Summary of the Invention

[0015] To address the aforementioned problems, the first objective of this invention is to provide a long-lasting deodorizing cat litter composition comprising probiotics, a compound enzyme preparation, polypeptides, a diatomaceous earth carrier, lemongrass essential oil, and trehalose.

[0016] In some embodiments, the long-lasting deodorizing cat litter composition comprises, by weight percentage, 1-5% probiotics, 0.5-2% compound enzyme preparation, 0.01-0.05% polypeptides, 85-95% diatomaceous earth carrier, 0.1-0.5% lemongrass essential oil and 0.5-2% trehalose.

[0017] In some embodiments, the probiotics include Lactobacillus rhamnosus (ATCC53103) and Lactobacillus salivarius (CICC23174).

[0018] In some embodiments, the amino acid sequence of the polypeptide is EQKMFQIVGG.

[0019] In some embodiments, the complex enzyme preparation comprises cellulase, protease, and chitinase.

[0020] In some embodiments, the long-lasting deodorizing cat litter composition contains an enzyme-activating protectant.

[0021] In some embodiments, the enzyme activity protectant is trehalose or polyethylene glycol.

[0022] The second objective of this invention is to provide an application of the above-mentioned pet-use long-lasting deodorizing cat litter composition, wherein the application is to prepare a product for inhibiting cat-related pathogens and mites;

[0023] In some embodiments, the mite is the feline anal mite.

[0024] Compared with the prior art, the present invention has at least the following beneficial effects:

[0025] This invention relates to the field of pet cat litter technology, specifically to a long-lasting deodorizing cat litter composition, its preparation method, and its application. The composition comprises probiotics (Lactobacillus rhamnosus (ATCC53103) and Lactobacillus salivarius (CICC23174), 1-5%), a compound enzyme preparation (cellulase + protease + chitinase, 0.5-2%), and a diatomaceous earth carrier (85-95%). This composition inhibits pathogenic bacteria such as Escherichia coli and Staphylococcus aureus through the secretion of bacteriocins by the probiotics, and the compound enzymes directionally decompose odor-causing substances such as hydrogen sulfide and ammonia. This long-lasting deodorizing cat litter composition solves the problems of poor safety, limited functionality, and insufficient long-lasting effect of traditional cat litter chemical additives, and is suitable for the field of pet excrement treatment. Attached Figure Description

[0026] Figure 1 This is a diagram for identifying the amino acid sequence of the polypeptide. Detailed Implementation

[0027] To make the technical problems, technical solutions and advantages of the present invention clearer, a detailed description will be given below in conjunction with the accompanying drawings and specific embodiments.

[0028] Lemongrass essential oil was purchased from Jiangxi Wanhua Fragrance Co., Ltd.

[0029] Escherichia coli (ATCC 25922), Lactobacillus rhamnosus (ATCC53103), and Lactobacillus salivarius (CICC23174) were purchased from the China Industrial Microbial Culture Collection Center.

[0030] Example 1: Extraction of Artemisia argyi polypeptide:

[0031] (1) Take 100g of fresh Artemisia argyi H.Lév. & Vaniot tender leaves, wash with ddH2O to remove mud and impurities, drain and cut into pieces for easy grinding; quickly place the cut plant tissue into liquid nitrogen to freeze it completely; place the frozen plant tissue into a pre-cooled mortar, add a small amount of liquid nitrogen, and grind quickly into a fine powder. During grinding, avoid the liquid nitrogen from drying out to ensure that the sample is fully broken down. Soak in 2 times the volume of PBS buffer, stir for 2 hours, filter with two layers of gauze, and retain the residue;

[0032] (2) Transfer the filter residue from step (1) to a large beaker, add 500 mL of deionized water to the large beaker, then place the beaker in a water bath, stir at 55°C for 2 hours, then cool to 45°C and add a compound enzyme preparation (weight ratio of 1:1:1) consisting of cellulase (100000 u / g), papain (200000 u / g) and neutral protease (500000 u / g). The amount of compound enzyme is 1% of the weight of the raw material. Enzymatic hydrolysis is carried out at pH 6.5 and temperature 50°C for 3 hours; centrifuge at 3000g for 15 min to obtain the supernatant.

[0033] (3) The supernatant was concentrated by filtration using membranes with molecular weight cutoffs of 2 kDa and 0.5 kDa, either individually or in combination, to separate and purify peptide solutions of three molecular weights: A (>2 kDa), B (<0.5 kDa), and C (0.5 kDa-2 kDa). The peptide solutions and supernatant of the above three molecular weights were collected separately and desalted using a dialysis bag (100 Da). After concentration by rotary evaporation, they were freeze-dried to obtain four types of lyophilized powders (A, B, C, and supernatant), which were stored separately at -20°C for later use.

[0034] Example 2

[0035] The four freeze-dried powders prepared in Example 1 were tested according to T / CIAA017-2022 "Test Method and Evaluation of Anti-mite and Anti-mite Performance of Household Cleaning Products" (the standard test uses dust mites, house dust mites, or nymphs). Additionally, cat anal mites were tested. The samples from Example 1 of this invention were exposed to the solution for 48 hours, and the number of dead mites was recorded and observed to examine their anti-mite performance. The test results are shown in Table 1 below:

[0036] Table 1. Acaricide effect of each component

[0037] Component (20 ng / ul) Dust mite eradication rate (%) Cat anal mite eradication rate (%) A 42.3±1.4 18.1±0.4 B 31.1±0.2 10.6±0.8 C 91.3±1.2 50.6±2.1 Supernatant 60.1±2.1 30.1±1.2

[0038] As shown in Table 1, the C (0.5 kDa-2 kDa) lyophilized powder had a significantly higher mite-killing effect compared to other groups (p < 0.01). Mass spectrometry analysis of the C component revealed its amino acid sequence to be EQKMFQIVGG. (See Table 1 for details.) Figure 1 .

[0039] Example 3: Preparation of cat litter composition

[0040] Cat litter composition formula (by weight): probiotics (3%) + compound enzyme preparation (1.5%) + polypeptide EQKMFQIVGG freeze-dried powder (0.02%) + diatomaceous earth (93.98%) + lemongrass essential oil (0.2%) + trehalose (1.3%);

[0041] The probiotic is Lactobacillus rhamnosus (ATCC53103) (live count ≥ 1.0 × 10⁻⁶). 11 CFU / g) and Lactobacillus salivarius (CICC23174) (live count ≥1.0×10⁻⁶) 11 CFU / g), calculated by weight as 1:1.

[0042] The probiotic combination showed the following antibacterial properties: an inhibition zone diameter of 18.2 ± 0.5 mm against *Escherichia coli* (ATCC 25922) and 18.0 ± 0.6 mm against *Staphylococcus aureus* (ATCC 6538). Acid resistance test: The survival rate of the strains was ≥85% in an environment with pH 3.0-6.0 (determined by plate counting method).

[0043] The compound enzyme preparation comprises cellulase (2500 U / g), protease (1500 U / g), chitinase (1800 U / g), and polyethylene glycol (2% w / w), wherein the cellulase, protease, and chitinase are in a weight ratio of 1:1:1. The compound has undergone stability testing: after being stored at 4°C for 7 days, the enzyme activity retention rate is ≥80%.

[0044] (2) Granulation process:

[0045] (2.1) Mixing: Add each component to a double helix cone mixer and mix for 30 minutes.

[0046] (2.2) Granulation: Granulation is carried out by a disc granulator (2mm aperture). The granules are dried at 50℃ for 2 hours and then screened to obtain uniform granules.

[0047] Example 4

[0048] The difference from Example 3 is that the formula (by weight) is: probiotics (2.5%) + compound enzyme preparation (2%) + polypeptide EQKMFQIVGG freeze-dried powder (0.02%) + diatomaceous earth (93.98%) + lemongrass essential oil (0.2%) + trehalose (1.3%).

[0049] Example 5

[0050] The difference from Example 3 is that the formula (by weight) is: probiotics (3.5%) + compound enzyme preparation (1%) + polypeptide EQKMFQIVGG freeze-dried powder (0.02%) + diatomaceous earth (93.98%) + lemongrass essential oil (0.2%) + trehalose (1.3%).

[0051] Comparative Example 1

[0052] The difference from Example 3 is that probiotics are omitted, and the missing probiotic weight is replaced with an equal amount of diatomaceous earth, while the proportions of the remaining components remain unchanged.

[0053] Comparative Example 2

[0054] The difference from Example 3 is that only Lactobacillus rhamnosus (ATCC53103) is used in the probiotics, while the ratio of probiotics to other components remains unchanged.

[0055] Comparative Example 3

[0056] The difference from Example 3 is that only Lactobacillus salivarius (CICC23174) is used in the probiotics, while the ratio of probiotics to other components remains unchanged.

[0057] Comparative Example 4

[0058] The difference from Example 3 is that cellulase is omitted in the compound enzyme preparation, while the ratio of the compound enzyme preparation and the remaining components remains unchanged.

[0059] Comparative Example 5

[0060] The difference from Example 3 is that the protease is omitted in the compound enzyme preparation, while the ratio of the compound enzyme preparation and the remaining components remains unchanged.

[0061] Comparative Example 6

[0062] The difference from Example 3 is that chitinase is omitted from the compound enzyme preparation, while the ratio of the compound enzyme preparation and the remaining components remains unchanged.

[0063] Comparative Example 7

[0064] The difference from Example 3 is that the compound enzyme preparation is omitted, and the missing compound enzyme preparation is replaced by an equal amount of diatomaceous earth, while the proportions of the remaining components remain unchanged.

[0065] Comparative Example 8

[0066] The difference from Example 3 is that the polypeptide is omitted, and the missing polypeptide preparation is replaced with an equal amount of diatomaceous earth, while the proportions of the remaining components remain unchanged.

[0067] Example 6: Long-lasting performance test of cat litter

[0068] Experimental conditions

[0069] • Testing period: 14 days

[0070] • Environmental simulation: Temperature 25±2℃, humidity 60±5%. Cat litter from Examples 3-5, Comparative Examples 1-8, and commercially available ordinary diatomaceous earth cat litter were used. Fresh cat feces (containing Escherichia coli, Staphylococcus aureus, and mites) were added every 24 hours. The antibacterial rate, deodorization rate, and mite-killing rate were calculated on day 14. The antibacterial rate was calculated based on the content of Escherichia coli, and the deodorization rate was calculated based on the content of NH3 and H2S. The experimental results are shown in Table 2.

[0071] The control group consisted of commercially available ordinary diatomaceous earth cat litter;

[0072] Table 2. Long-lasting performance test of cat litter

[0073]

[0074] Table 2 shows that the cat litter of Examples 3-5 of the present invention has the best long-lasting deodorizing, antibacterial and anti-mite effect, which is significantly better than the control group (p<0.01).

[0075] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A long-lasting deodorizing cat litter composition, characterized in that, The long-lasting deodorizing cat litter composition contains, by weight percentage, 1-5% probiotics, 0.5-2% compound enzyme preparation, 0.01-0.05% polypeptides, 85-95% diatomaceous earth carrier, 0.1-0.5% lemongrass essential oil and 0.5-2% trehalose; The probiotics include Lactobacillus rhamnosus ATCC53103 and Lactobacillus salivarius CICC23174; The amino acid sequence of the polypeptide is EQKMFQIVGG; The compound enzyme preparation contains cellulase, protease, and chitinase.

2. The long-lasting deodorizing cat litter composition according to claim 1, characterized in that, The long-lasting deodorizing cat litter composition contains an enzyme-activating protectant.

3. The long-lasting deodorizing cat litter composition according to claim 2, characterized in that, The enzyme activity protectant is trehalose or polyethylene glycol.

4. The application of a long-lasting deodorizing cat litter composition as described in any one of claims 1-3, characterized in that, The application is to prepare products for inhibiting cat-related pathogens and mites; the mites are feline anal mites.

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