A hair pore astringent composition containing phellinus baumii and its preparation method and application

Abstract

This invention discloses a pore-tightening composition containing *Polyporus medica*, its preparation method, and its application, belonging to the field of cosmetic technology. The pore-tightening composition provided by this invention comprises the following components in parts by weight: 0.01-2 parts gentian root extract, 0.1-10 parts barley flour, 0.1-10 parts *Polyporus medica* extract, 0.01-5 parts red clover flower extract, and 0.01-5 parts flaxseed extract. By selecting appropriate parts by weight, this invention achieves synergistic effects among the components, effectively regulating sebum secretion, promoting keratinocyte metabolism, and promoting collagen regeneration. When applied to the human body, it effectively refines pores, reduces oil secretion, and enhances the skin barrier. Furthermore, the effects of refining pores, reducing oil secretion, and enhancing the skin barrier are relatively long-lasting, providing a long-lasting pore-refining effect. Moreover, the pore-tightening composition provided by this invention is gentle and non-irritating, suitable for people with sensitive skin.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and in particular relates to a pore-tightening composition containing medicinal fumonis, its preparation method and application. Background Technology

[0002] Large pores are a common skin problem in modern society, especially noticeable in oily and combination skin. With age, environmental pollution, and stress, pores gradually enlarge, reducing skin firmness and smoothness. This phenomenon is mainly related to excessive sebum secretion, buildup of keratin within pores, collagen loss, and decreased skin elasticity. In particular, excessive sebum secretion leads to its accumulation within pores, easily causing enlargement and blockage, which in turn triggers inflammation and blackheads.

[0003] Existing technologies and products targeting enlarged pores primarily rely on chemical ingredients to achieve astringent or exfoliating effects. Many products use formulas containing acids (such as salicylic acid and fruit acids). While effective, long-term use may damage the skin barrier or cause allergies, especially for those with sensitive skin. Furthermore, some products temporarily shrink pores using single physical astringent ingredients (such as alcohol or certain astringents), but the effects are not lasting and can irritate the skin. Therefore, developing a gentle pore-tightening composition that can regulate skin function from multiple angles—effectively refining pores without irritating the skin—is a pressing need in the current skincare industry. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a pore-tightening composition containing medicinal fumonis, which can effectively regulate sebum secretion, promote keratin metabolism around hair follicles and promote collagen regeneration, and when applied to the human body, can effectively refine pores, reduce sebum secretion and enhance the skin barrier function, as well as the preparation method and application thereof.

[0005] To achieve the above objectives, in a first aspect, the present invention provides a pore-tightening composition comprising the following components in parts by weight:

[0006] Gentian root extract 0.01-2 parts, barley flour 0.1-10 parts, medicinal fusiforme extract 0.1-10 parts, red clover flower extract 0.01-5 parts, flaxseed extract 0.01-5 parts.

[0007] This invention, by selecting appropriate proportions of components, achieves synergistic effects among the components, effectively regulating sebum secretion, promoting keratin metabolism around hair follicles, and promoting collagen regeneration. When applied to the human body, it effectively refines pores, reduces sebum secretion, and enhances the skin barrier. Moreover, the effects of refining pores, reducing sebum secretion, and enhancing the skin barrier are relatively long-lasting, providing a long-lasting pore-refining effect. Furthermore, the pore-refining composition provided by this invention is gentle and non-irritating, making it suitable for people with sensitive skin.

[0008] Specifically, gentian root extract is rich in gentianin, which has a significant immediate astringent effect, quickly shrinking skin pores and effectively relieving skin inflammation, preventing enlarged pores caused by inflammation. Barley flour is rich in natural polysaccharides, which can absorb excess oil and dirt on the skin surface through physical adsorption, helping to clean pores, keep skin fresh, and prevent pore blockage. Medicinal spore fungus extract contains abundant triterpenes and polysaccharides, effectively regulating sebum secretion, helping to balance the skin's oil and water levels, thereby reducing pore enlargement caused by excessive oil, and also has a repairing effect, repairing damaged skin barriers. Red clover flower extract is rich in natural plant polyphenols, which can promote keratin metabolism around hair follicles, helping to remove dead skin cells, preventing keratin buildup that can clog pores, and making skin smoother and more delicate. Flaxseed extract is rich in plant Omega-3 fatty acids and antioxidants, which can promote collagen regeneration, enhance skin elasticity, and repair enlarged pores caused by skin laxity. The inventors speculate that, in addition to the aforementioned effects and other effects not mentioned in this invention, the components also have unpredictable synergistic effects, resulting in excellent overall effects and significant benefits when compounded within the mass fraction range given in this invention.

[0009] As a preferred embodiment of the pore-tightening composition of the present invention, the pore-tightening composition comprises the following components in parts by weight: 0.05-0.5 parts of gentian root extract, 0.5-5 parts of barley flour, 1-5 parts of medicinal styracifolium extract, 0.1-3 parts of red clover flower extract, and 0.1-2 parts of flaxseed extract.

[0010] As a preferred embodiment of the pore-tightening composition of the present invention, the pore-tightening composition comprises the following components in parts by weight: 0.2-0.3 parts of gentian root extract, 1-2 parts of barley flour, 2-3 parts of medicinal styracifolium extract, 1-2 parts of red clover flower extract, and 0.5-1 parts of flaxseed extract.

[0011] The present invention has found that the mass fraction of the components has a significant impact on the overall performance of the pore-tightening composition. When the mass fraction of the components is further selected within the above-mentioned preferred and more preferred ranges, the overall effect of the obtained pore-tightening composition is even better.

[0012] As a preferred embodiment of the pore-tightening composition of the present invention, the preparation method of the red clover flower extract includes the following steps: adding dried and pulverized red clover flowers to an aqueous solution of a natural eutectic solvent for extraction, centrifuging after extraction, collecting the supernatant and concentrating it to obtain red clover flower extract; the natural eutectic solvent includes betaine and glycerol.

[0013] This invention has found that different extraction methods result in variations in the effective substances and their content in the extracts of red clover flowers, thus affecting the interaction between the red clover flower extract and other components. Extensive research has shown that when extracted using a natural eutectic solvent composed of betaine and glycerin as described in this invention, subsequent addition to the pore-refining composition results in a more effective pore-refining composition that reduces sebum secretion, promotes keratin metabolism around hair follicles, and stimulates collagen regeneration, thereby achieving a better effect on refining pores.

[0014] In a preferred embodiment of the pore-tightening composition of the present invention, the mass ratio of betaine to glycerin is 1:(2-4).

[0015] For example, the mass ratio of betaine to glycerol can be any point value or any two points between 1:(2-4), such as 1:2, 1:2.5, 1:3, 1:3.5, 1:4, etc.

[0016] In a preferred embodiment of the pore-tightening composition of the present invention, the natural eutectic solvent aqueous solution contains 70-80% by mass of the natural eutectic solvent.

[0017] For example, in the aqueous solution of the natural eutectic solvent, the mass percentage of the natural eutectic solvent can be any point value or any two points between 70% and 80%, such as 70%, 72%, 74%, 76%, 78%, 80%, etc.

[0018] As a preferred embodiment of the pore-tightening composition of the present invention, the mass-to-volume ratio of the red clover flower and the natural eutectic solvent aqueous solution is (30-50) mg: 1 mL.

[0019] For example, the mass-volume ratio of the red clover flowers and the natural eutectic solvent aqueous solution can be any point value or any two points between (30-50) mg:1 mL, such as 30 mg:1 mL, 35 mg:1 mL, 40 mg:1 mL, 45 mg:1 mL, 50 mg:1 mL, etc.

[0020] It should be noted that the present invention does not limit the extraction method; any conventional extraction method in the art can be used, such as ultrasonic extraction, heating reflux extraction, or microwave extraction.

[0021] For example, when ultrasonic extraction is used, the extraction temperature is 40-60℃, the ultrasonic frequency is 80-120W, and the extraction time is 25-45min.

[0022] For example, when using heating reflux extraction, the extraction temperature is 60-80℃ and the time is 0.5-1.5h.

[0023] For example, when microwave extraction is used, the microwave power is 200-400W and the extraction time is 10-20min.

[0024] In a preferred embodiment of the pore-tightening composition of the present invention, the average particle size of the barley flour is 140-160 μm.

[0025] It should be noted that the average particle size of the barley flour was measured using a microscope.

[0026] For example, the average particle size of the barley flour can be any point value or any two points between 140-160μm, such as 140μm, 142μm, 145μm, 148μm, 150μm, 152μm, 155μm, 158μm, 160μm, etc.

[0027] As a preferred embodiment of the pore-tightening composition of the present invention, the sum of the mass of the barley flour and the medicinal Fomitopsis pinnatifida extract accounts for 56-75% of the total mass of the pore-tightening composition.

[0028] The present invention has found that, by further balancing the mass ratio of the components in the pore-tightening composition, and selecting a total mass percentage of 56-75% for the barley flour and medicinal Fomitopsis pilosula extract in the pore-tightening composition, the overall effect of the resulting pore-tightening composition is better.

[0029] For example, the percentage of the sum of the barley flour and the medicinal Fomitopsis extract, based on the total mass of the pore-tightening composition, can be any point value or any two-point range value between 56% and 75%, such as 56%, 58%, 60%, 62%, 64%, 66%, 68%, 70%, 72%, 75%, etc.

[0030] Preferably, the total mass of the barley flour and the medicinal Fomitopsis extract accounts for 58-63% of the total mass of the pore-tightening composition.

[0031] In a second aspect, the present invention provides the use of the pore-tightening composition in the preparation of cosmetics.

[0032] The pore-tightening composition provided by this invention can effectively regulate sebum secretion, promote keratin metabolism and collagen regeneration around hair follicles, and thus can be widely used in the preparation of cosmetics to achieve long-lasting and powerful pore-refining effects, reduce sebum secretion, and enhance skin barrier function.

[0033] As a preferred embodiment of the application described in this invention, the cosmetic includes any one of toner, lotion, cream, mask, serum, and spray.

[0034] In a third aspect, the present invention provides an emulsion comprising the following components by weight percentage: 0.05-0.3% thickener, 0.5-1% humectant, 0.01-0.3% pH adjuster, 0.5-5% emulsifier, 0.5-3% preservative, 1-10% of the pore-tightening composition, and the balance being deionized water.

[0035] As a preferred embodiment of the emulsion of the present invention, the emulsion comprises the following components by mass percentage: 0.18-0.22% thickener, 0.8-0.95% humectant, 0.02-0.05% pH adjuster, 3.5-4.5% emulsifier, 1-1.2% preservative, 4-6% of the pore-tightening composition, and the balance being deionized water.

[0036] In a preferred embodiment of the emulsion described in this invention, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol.

[0037] In a preferred embodiment of the emulsion described in this invention, the moisturizer includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerin, budding cleavage enzyme polysaccharide, and ceramide.

[0038] In a preferred embodiment of the emulsion described in this invention, the pH adjuster includes at least one of arginine, tromethamine, and disodium EDTA.

[0039] In a preferred embodiment of the emulsion described in this invention, the emulsifier includes at least one of the following: caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate.

[0040] In a preferred embodiment of the emulsion described in this invention, the preservative includes at least one of 1,2-propanediol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylate / C10-30 alkanol acrylate crosslinking polymers.

[0041] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0042] This invention, by selecting appropriate proportions of components, achieves synergistic effects among the components, effectively regulating sebum secretion, promoting keratin metabolism around hair follicles, and promoting collagen regeneration. When applied to the human body, it effectively refines pores, reduces sebum secretion, and enhances the skin barrier. Moreover, the effects of refining pores, reducing sebum secretion, and enhancing the skin barrier are relatively long-lasting, providing a long-lasting pore-refining effect. Furthermore, the pore-refining composition provided by this invention is gentle and non-irritating, making it suitable for people with sensitive skin. Detailed Implementation

[0043] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0044] Unless otherwise specified, the reagents, methods and equipment used in this invention are all conventional reagents, methods and equipment in the field; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch.

[0045] Gentian root extract: purchased from Shaanxi Haochen Biotechnology Co., Ltd.

[0046] Medicinal Fomitopsis extract: purchased from BASF, Germany.

[0047] Flaxseed extract: purchased from Shaanxi Yinuo Biotechnology Co., Ltd.

[0048] Barley flour 1: Average particle size is 140μm;

[0049] Barley flour 2: Average particle size is 160μm;

[0050] Barley flour 3: Average particle size is 120μm;

[0051] Barley flour 4: Average particle size is 180μm;

[0052] Barley flour 1-4 were prepared by grinding and sieving barley flour purchased from Baoji Liupanyun Biotechnology Co., Ltd.

[0053] Red clover flower extract 1: Homemade, the preparation method includes the following steps:

[0054] (1) Collect the flowers of red clover, clean them, dry them in a 50℃ constant temperature oven until constant weight, then take them out, grind them into powder and pass them through an 80-mesh sieve, and collect the sieve material.

[0055] (2) Take 1g of the sieved material and add it to 20mL of natural eutectic solvent aqueous solution (the natural eutectic solvent is 75% by mass based on the total mass of the natural eutectic solvent aqueous solution; the natural eutectic solvent is composed of betaine and glycerol in a mass ratio of 1:3) and extract it by ultrasonic extraction at 100W power and 50℃ for 35min.

[0056] (3) After ultrasonic extraction, centrifuge, collect the supernatant and concentrate under reduced pressure to obtain red clover flower extract 1;

[0057] Red clover flower extract 2: self-made, the only difference between the preparation method and red clover flower extract 1 is that in step (2) the extraction is carried out by microwave at 300W for 15 minutes.

[0058] Red clover flower extract 3: Homemade, the only difference between the preparation method and red clover flower extract 1 is that the natural eutectic solvent is composed of betaine and glycerin in a mass ratio of 1:0.6;

[0059] Red clover flower extract 4: Homemade, the only difference between the preparation method and red clover flower extract 1 is that the natural eutectic solvent is composed of betaine and glycerin in a mass ratio of 1:8.

[0060] Red clover flower extract 5: Homemade, the only difference between the preparation method and that of red clover flower extract 1 is that the mass percentage of the natural eutectic solvent is 90% based on the total mass of the aqueous solution of the natural eutectic solvent.

[0061] Red clover flower extract 6: Homemade, the only difference between the preparation method and red clover flower extract 1 is that the natural eutectic solvent is composed of glycerol and choline chloride in a mass ratio of 1:3;

[0062] Red clover flower extract 7: Homemade, the only difference between this preparation method and that of red clover flower extract 1 is that a 75% ethanol aqueous solution is used instead of a natural eutectic solvent aqueous solution.

[0063] Examples 1-7 and Comparative Examples 1-8

[0064] The present invention provides a pore-tightening composition in the embodiments and comparative examples, the components (parts by mass) of the pore-tightening composition are shown in Table 1;

[0065] Table 1

[0066]

[0067]

[0068] The method for preparing the pore-tightening composition provided in Example 1 is as follows: the components are mixed evenly to obtain the pore-tightening composition.

[0069] The preparation methods of the pore-tightening compositions provided in Examples 2-7 and Comparative Examples 1-8 are consistent with those in Example 1.

[0070] Example 8

[0071] This invention provides a pore-tightening composition, the only difference between the pore-tightening composition and Example 1 is that barley flour 2 is used instead of barley flour 1.

[0072] Example 9

[0073] This invention provides a pore-tightening composition, the only difference between the pore-tightening composition and Example 1 is that barley flour 3 is used instead of barley flour 1.

[0074] Example 10

[0075] This invention provides a pore-tightening composition, the only difference between the pore-tightening composition and Example 1 is that barley flour 4 is used instead of barley flour 1.

[0076] Example 11

[0077] This invention provides a pore-tightening composition, the only difference between the pore-tightening composition and Example 1 is that red clover flower extract 2 is used instead of red clover flower extract 1.

[0078] Example 12

[0079] This invention provides a pore-tightening composition, the only difference between the pore-tightening composition and Example 1 is that red clover flower extract 3 is used instead of red clover flower extract 1.

[0080] Example 13

[0081] This invention provides a pore-tightening composition, the only difference between the pore-tightening composition and Example 1 is that red clover flower extract 4 is used instead of red clover flower extract 1.

[0082] Example 14

[0083] This invention provides a pore-tightening composition, the only difference between the pore-tightening composition and Example 1 is that red clover flower extract 5 is used instead of red clover flower extract 1.

[0084] Example 15

[0085] This invention provides a pore-tightening composition, the only difference between the pore-tightening composition and Example 1 is that red clover flower extract 6 is used instead of red clover flower extract 1.

[0086] Example 16

[0087] This invention provides a pore-tightening composition, the only difference between the pore-tightening composition and Example 1 is that red clover flower extract 7 is used instead of red clover flower extract 1.

[0088] Comparative Example 9

[0089] The present invention provides a pore-tightening composition in a comparative example, the only difference between the pore-tightening composition and Example 1 is that Coptis chinensis extract (purchased from Shaanxi Huike Plant Development Co., Ltd.) is used instead of Gentiana scabra root extract.

[0090] Comparative Example 10

[0091] The present invention provides a pore-tightening composition in a comparative example. The only difference between the pore-tightening composition and Example 1 is that oat flour (purchased from Shaanxi Angsheng Biomedical Technology Co., Ltd.) is used instead of barley flour 1.

[0092] Comparative Example 11

[0093] The present invention provides a pore-tightening composition in a comparative example. The only difference between the pore-tightening composition and Example 1 is that the medicinal F. sp. 'Ceratophyllum demersum' (purchased from Shaanxi Haosen Biotechnology Co., Ltd.) is used instead of the medicinal F. sp. 'Ceratophyllum demersum' extract.

[0094] Comparative Example 12

[0095] The present invention provides a pore-tightening composition in a comparative example. The only difference between the pore-tightening composition and Example 1 is that Lysimachia christinae extract (purchased from Shaanxi Huike Plant Development Co., Ltd.) is used instead of Trifolium repens flower extract 1.

[0096] Comparative Example 13

[0097] The present invention provides a pore-tightening composition in a comparative example, the only difference between the pore-tightening composition and Example 1 is that grape seed extract (purchased from Shaanxi Huike Plant Development Co., Ltd.) is used instead of flaxseed extract.

[0098] Application Example 1-18, Comparative Application Example 1-14

[0099] The present invention provides an emulsion in application examples and comparative application examples, the components (mass percentage) of which are shown in Table 2; wherein, the pore-tightening compositions used in application examples 1-16 are the pore-tightening compositions prepared in examples 1-16 respectively, and the pore-tightening compositions used in comparative application examples 1-13 are the pore-tightening compositions prepared in comparative examples 1-13 respectively; the pore-tightening compositions used in application examples 17-18 are the pore-tightening compositions prepared in example 1.

[0100] Table 2

[0101]

[0102]

[0103] The method for preparing the emulsion provided in Example 1 includes the following steps:

[0104] (1) Mix the humectant, thickener and part of the pH adjuster (EDTA-disodium) with water and stir. Heat to 85°C and homogenize at 1300 rpm for 4 min. After homogenization, keep warm for later use to obtain pre-prepared component A.

[0105] (2) Mix the emulsifier, heat to 75°C, and homogenize at 1300 rpm for 4 min. After homogenization, keep warm for later use to obtain the pre-prepared component B.

[0106] (3) Mix the preservatives and heat to 60°C to melt them to obtain pre-prepared component C;

[0107] (4) Heat the pre-prepared component A to 80°C, add the pre-prepared component B at 300 rpm, stir and mix, then cool down to 60°C, add the pre-prepared component C at 300 rpm and stir and mix, then cool down to below 45°C, add the pore-tightening composition and continue stirring for 8 minutes, finally add the remaining pH adjuster (arginine) to adjust the pH to 5.5-7.0, then stop stirring, discharge the material, and obtain the emulsion;

[0108] The preparation method of the emulsion provided in Application Example 2-18 is consistent with that in Application Example 1-14.

[0109] Example 1

[0110] The efficacy of the pore-tightening compositions prepared in Examples 1-16 and Comparative Examples 1-13 of this invention is investigated in the following aspects:

[0111] 1. Ability to regulate sebum secretion

[0112] The cell line used was human sebaceous gland cells SZ95 (Shanghai Qingqi Biotechnology, BFN60807569), passaged 7 times. The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured according to groups, followed by testing, specifically a quantitative PCR assay for neutral lipids. The testing method is as follows:

[0113] (1) Seed the cell suspension into 96-well cell culture plates at a density of 1.0 x 10⁶ cells per well. 4 Add 100 μL of DMEM medium (Gibco, C11965500BT) to each well and incubate for 18-24 h.

[0114] (2) Discard the supernatant. Add 100 μL of culture medium containing 10 ng / mL hydrocortisone (Shanghai Yuanye Biotechnology, S31360) to the control group. Add 100 μL of culture medium containing 10 ng / mL hydrocortisone and 0.2% w / v (g / mL) of the pore-tightening composition prepared in the examples and comparative examples to the sample groups respectively.

[0115] (3) After incubation for 48 hours, the supernatant was discarded, and the mixture was washed twice with PBS. Nile red dye (Shanghai Yuanye Biotechnology, S02N11G129713) was added and incubated at 37°C in the dark for 15 minutes. The mixture was washed twice with PBS and the fluorescence was detected using a multi-functional microplate reader. The excitation wavelength was 485 nm and the absorption wavelength was 565 nm.

[0116] (4) Results were calculated using fluorescence OD values. The reduction rate of intracellular neutral lipids (%) = (1 - OD value of sample group / OD value of control group) * 100%;

[0117] The results are shown in Table 3.

[0118] 2. Ability to promote the metabolism of keratinocytes around hair follicles

[0119] Experimental materials: The cell line used was human keratinocytes (HaCaT, Guangzhou Genio Biotechnology Co., Ltd.). The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by testing, specifically the expression detection of keratin metabolism-related genes KLK5 and Caspase-14. The specific testing methods are as follows:

[0120] (1) The cell suspension was seeded into a 96-well cell culture plate at a density of 2000 cells / well, and 100 μL of culture medium was added to each well. The cells were cultured for 24 h.

[0121] (2) Discard the supernatant, add 100 μL of DMEM medium (2105341, Gibco) to the blank group, and add 100 μL of medium containing 0.2% w / v (g / mL) of the pore-tightening composition prepared in the examples and comparative examples to the sample groups respectively.

[0122] (3) After incubation for 48 hours, the supernatant was discarded, and RNA was extracted from the cells using an RNA extraction kit (TRIzol, Thermo Fisher Scientific). The RNA was then reverse transcribed into cDNA using a reverse transcription kit (RevertAid RT, Thermo Fisher Scientific). The synthesized cDNA was used as a template for real-time quantitative PCR (TaqMan, Thermo Fisher Scientific).

[0123] (4) The expression levels of KLK5 and Caspase-14 genes in HaCaT cells of each group were detected by real-time quantitative PCR. GAPDH was used as an internal reference gene. The KLK5 primer sequence used was:

[0124] 5'-CCTGTTCTTGCTGGGGATGT-3',

[0125] 5'-ACTTGGAATCCGTGCTGAGG-3';

[0126] The Caspase-14 primer sequence used is:

[0127] 5'-GATGAGGTTGCTGTGCTAA-3',

[0128] 5'-GAACACATCCGTCAGGGTCT-3';

[0129] The GAPDH primer sequences used are:

[0130] 5'-ACTTTGTCAAGCTCATTTCC-3',

[0131] 5'-TGCAGCGAACTTTATTGATG-3';

[0132] Kleptin protease KLK5 promotes the shedding of keratinocytes, while caspase-14 promotes apoptosis and maturation of keratinocytes; both work together to promote keratinocyte renewal. Therefore, after sample treatment, higher expression levels of KLK5 and caspase-14 indicate a higher level of keratinocyte metabolism and a stronger ability of the composition to promote keratinocyte metabolism. The ability of the composition to promote keratinocyte metabolism is represented by the increase in the expression levels of KLK5 and caspase-14 genes, calculated using the following formula:

[0133] KLK5 / Caspase-14 gene expression increase rate = (gene expression level) 样品组 / Gene expression level 空白组 (×100%)-1;

[0134] The results are shown in Table 3.

[0135] 3. Ability to promote collagen regeneration

[0136] The cell line used was human dermal fibroblast HDF (catalog number PC-202h, Wuhan Saisuo Biotechnology Co., Ltd.). The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by testing, specifically the determination of type I collagen content. The specific testing method is as follows:

[0137] (1) Inoculation: Inoculate cells into culture flasks at a density of 1×10⁶ cells / year. 6 The cells / mL were incubated in DMEM high glucose medium (Gibco) containing 10% fetal bovine serum at 37°C for 24 h in a 5% CO2 incubator.

[0138] (2) Induction: Treat with PBS buffer containing 50 μM hydrogen peroxide for 30 minutes, discard the PBS buffer containing hydrogen peroxide, and then wash with PBS 3 times.

[0139] (3) Sample feeding: The blank group was added with DMEM high glucose culture medium containing 10% fetal bovine serum, and the sample group was added with DMEM high glucose culture medium containing 10% fetal bovine serum and 0.2% w / v (g / mL) of the pore-tightening composition prepared in the examples and comparative examples. The cells were collected after being cultured in a 37°C, 5% CO2 incubator for 24 hours.

[0140] (4) Detection of type I collagen content: Cells were lysed with RIPA cell lysis buffer containing protease inhibitors. The lysed samples were centrifuged at 12000g for 5 minutes, and the supernatant was collected. The type I collagen content of the cell supernatant in each group was detected using a human type I collagen (Col I) ELISA kit (catalog number ml057630, ELISA).

[0141] The higher the collagen content, the stronger the collagen regeneration promotion ability of the composition. The collagen regeneration promotion ability of the composition is represented by the increase in type I collagen, and the calculation formula is as follows: Collagen increase rate (%) = (Sample group - Blank group) / Blank group * 100%; the results are shown in Table 3.

[0142] Table 3

[0143]

[0144]

[0145] As can be seen from Table 3, when the technical solution provided by this invention is adopted, the obtained pore-tightening composition has excellent ability to regulate sebum secretion, promote keratinocyte metabolism, and promote collagen regeneration. Specifically, the obtained pore-tightening composition showed a neutral lipid reduction rate of over 15.3% in the sebum regulation test; in promoting keratinocyte metabolism, the KLK5 gene expression increase rate and the Caspase-14 gene expression increase rate were over 18.5% and 59.0%, respectively; and in promoting collagen production, the collagen increase rate was over 11.9%.

[0146] As can be seen from Examples 1-7, the mass fraction of the components and the mass percentage of some components in the pore-tightening composition will affect the overall performance of the pore-tightening composition. When the mass fraction of the components is further selected within the range given in this invention, the overall performance of the obtained pore-tightening composition is better, wherein the neutral lipid reduction rate is above 32.3%, the KLK5 gene expression increase rate and the Caspase-14 gene expression increase rate are above 35.9% and 123.2% respectively, and the collagen increase rate is above 23.1%.

[0147] As can be seen from Examples 1 and 8-10, the selection of parameters in the components also affects the overall performance of the pore-tightening composition. When the average particle size of the barley flour is within the preferred range of the present invention, the overall performance of the obtained pore-tightening composition is better. As can be seen from Examples 1 and 11-16, the preparation method of red clover flower extract also affects the overall performance of the pore-tightening composition.

[0148] As can be seen from Examples 1 and Comparative Examples 1-8, the effects of the present invention cannot be achieved when one or two of the components are not added, or when a component is not added and its mass fraction is added to another component; as can be seen from Examples 1 and Comparative Examples 9-13, the effects of the product obtained also show a certain downward trend when other components are used to replace the components in the present invention.

[0149] Example 2

[0150] The safety of the emulsions prepared in Application Examples 1-18 and Comparative Application Examples 1-14 was investigated using human skin patch tests, which included the following:

[0151] Thirty volunteers were recruited, 15 men and 15 women, aged 20-50 years. A closed patch test method was used. Equal volumes (0.020-0.025 mL) of the test sample were placed in a specific patch applicator, which was then applied to the volunteer's arm with hypoallergenic adhesive tape. The patch was gently pressed to ensure even application to the skin and left for 24 hours. The control group received distilled water. After 24 hours, the patch applicator was removed, and skin reactions were observed and recorded at 0.5 hours, 24 hours, and 48 hours. The severity of adverse skin reactions is shown in Table 4 below.

[0152] Table 4

[0153]

[0154] After testing, the lotions provided in the application examples and comparative application examples of this invention showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin.

[0155] Example 3

[0156] The efficacy of the emulsions prepared by the present invention in application examples 1-18 and comparative application examples 1-14 on the human body includes the following:

[0157] According to the "Cosmetic Safety Technical Specifications" (2015), 192 Asian adults aged 18-60 with sensitive skin were selected for the trial and randomly divided into 32 groups of 6 people each. Volunteers applied the sample (approximately 1-2g of lotion prepared from the application and control application examples) to their entire face twice daily, morning and evening. Data was collected on days 0, 7, and 28, and on day 5 after discontinuation of use. After each visit, volunteers washed their faces with facial cleanser and sat quietly for 30 minutes in an air-conditioned room with a temperature of 21±1℃ and humidity of 50±10%. The diameter of pores on the outer side of the nasolabial folds was measured using a digimiC800 skin image analyzer; the sebum content on the forehead was collected using a Sebumeter skin oil test probe; and the transepidermal water loss (TEWL) value on the cheekbone was measured using a TewaMeter skin moisture loss test probe.

[0158] The ability of a lotion to refine pores is represented by the reduction in pore size; its oil-controlling ability is represented by the reduction in forehead oil content; and its soothing and repairing ability is represented by the reduction in TEWL value. The formulas are as follows:

[0159] Improvement rate = (T0) 样品组 -Tx 样品组 ) / T0 样品组 *100%]-[(T0 对比应用例14 -Tx 对比应用例14 ) / T0 对比应用例14 *100%;

[0160] The results are shown in Table 5.

[0161] Table 5

[0162]

[0163]

[0164] As can be seen from Table 5, when the technical solution provided by this invention is adopted, the resulting emulsion has a long-lasting and powerful ability to refine pores, improve oil content, and enhance the skin barrier. Specifically, after 28 days of continuous use, the resulting emulsion showed an improvement rate of over 18.7% in pore diameter, over 15.8% in forehead oil content, and over 11.9% in TEWL (Treating and Reducing Oil Content). Five days after discontinuing use, the improvement rate of pore diameter was over 14.0%, the improvement rate of forehead oil content was over 11.7%, and the TEWL improvement rate was over 9.0%.

[0165] As can be seen from Application Examples 1-7, the mass fraction of the components in the added pore-tightening composition and the mass percentage of certain components in the pore-tightening composition both affect the efficacy of the pore-tightening composition in the emulsion, thereby affecting the overall performance of the emulsion. When the mass fraction of the components in the pore-tightening composition is further selected within the range given in this invention, the resulting emulsion has better overall performance. Specifically, after 28 days of continuous use, the resulting emulsion shows an improvement rate of over 37.5% in pore diameter, an improvement rate of over 32.4% in forehead oil content, and an improvement rate of over 22.1% in TEWL (Temperature-Reduced Oil). After 5 days of discontinuation of use, the improvement rate of pore diameter is over 32.0%, the improvement rate of forehead oil content is over 27.9%, and the improvement rate in TEWL is over 20.1%.

[0166] As can be seen from Application Example 1 and Comparative Application Examples 1-8, the effect of the present invention cannot be achieved when one or two of the components are not added to the pore-tightening composition, or when the mass fraction of one component is added to another component without adding the other component. As can be seen from Application Example 1 and Comparative Application Examples 9-13, the effect of the product also shows a certain downward trend when other components are used to replace the components in the present invention.

[0167] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A pore-tightening composition, characterized in that, The pore-tightening composition comprises the following components in parts by weight: Gentian root extract 0.05-0.5 parts, barley flour 0.5-5 parts, medicinal fusiforme extract 1-5 parts, red clover flower extract 0.1-3 parts, flaxseed extract 0.1-2 parts; The total mass of the pore-tightening composition comprises 56-75% of the sum of the mass of the barley flour and the medicinal Fomitopsis pinnatifida extract. The preparation method of the red clover flower extract includes the following steps: The dried and pulverized red clover flowers were added to a natural eutectic solvent aqueous solution for extraction. After extraction, the mixture was centrifuged, the supernatant was collected and concentrated to obtain red clover flower extract. The natural eutectic solvents include betaine and glycerol.

2. The pore-tightening composition according to claim 1, characterized in that, The pore-tightening composition comprises the following components in parts by weight: Gentian root extract 0.2-0.3 parts, barley flour 1-2 parts, medicinal fusiforme extract 2-3 parts, red clover flower extract 1-2 parts, flaxseed extract 0.5-1 parts.

3. The pore-tightening composition according to claim 1, characterized in that, The mass ratio of betaine to glycerol is 1:(2-4); And / or, in the aqueous solution of the natural eutectic solvent, the mass percentage of the natural eutectic solvent is 70-80%; And / or, the mass-to-volume ratio of the red clover flowers and the natural eutectic solvent aqueous solution is (30-50) mg: 1 mL.

4. The pore-tightening composition according to claim 1, characterized in that, The average particle size of the barley flour is 140-160 μm.

5. The use of the pore-tightening composition according to any one of claims 1-4 in the preparation of cosmetics.

6. An emulsion, characterized in that, The emulsion comprises the following components by weight percentage: 0.05-0.3% thickener, 0.5-1% moisturizer, 0.01-0.3% pH adjuster, 0.5-5% emulsifier, 0.5-3% preservative, 1-10% pore-tightening composition as described in any one of claims 1-4, and the balance being deionized water.

7. The emulsion according to claim 6, characterized in that, Satisfy at least one of the following: (1) The thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, ammonium acryloyl dimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; (2) The moisturizer includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerin, budding stalk polysaccharide, and ceramide; (3) The pH adjuster includes at least one of arginine, tromethamine, and disodium EDTA; (4) The emulsifier includes at least one of the following: caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate; (5) The preservative includes at least one of 1,2-propanediol, 1,2-hexanediol and p-hydroxyacetophenone.

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