A method for artificial planting and management of trillium govanianum wall.

Abstract

The application provides a dew grass artificial planting management method and relates to the dew grass planting technical field. The dew grass artificial planting management method mainly comprises the following steps: after the dew grass seeds are disinfected, the dew grass seeds are treated by fumigation with alpha-terpineol; pea vine extract is prepared by soaking pea vines in salt water, grinding, filtering and drying; and the dew grass is treated with the pea vine extract and other fertilizers during the seedling period and the transplanting period. The application overcomes the defects of the prior art, effectively improves the growth efficiency of the early dew grass seedlings, further promotes the growth and expansion of the dew grass roots and the accumulation of ecdysone, and comprehensively improves the yield and quality of the artificial dew grass planting.

Description

Technical Field

[0001] This invention relates to the field of dewgrass cultivation technology, specifically to a method for the artificial cultivation and management of dewgrass. Background Technology

[0002] Dewdrop, also known as pearl dewdrop, lung-changing powder, and cockscomb ginseng, is a perennial herb belonging to the genus *Cymbidium* of the Commelinaceae family. In my country, it mainly grows in mountain valleys, forest edges, or forests at altitudes of 1100-2700m in Yunnan, Guizhou, Guangxi, Guangdong, and Fujian provinces. Its main active part is the root, which contains ecdysone, a hormone that promotes cell growth, stimulates dermal cell division, and promotes human protein synthesis. It has good prospects in the medical field.

[0003] Because of the high overall survival rate of dewgrass, most existing dewgrass cultivation practices are quite extensive, usually involving direct sowing in the field. There is a lack of detailed research on the actual cultivation and management of dewgrass, which easily leads to inconsistent yields and quality in artificial cultivation, affecting the economic benefits of actual cultivation. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a method for the artificial cultivation and management of Dewgrass, which effectively promotes the growth of Dewgrass roots and further enhances the accumulation of molting hormones in the roots, thereby comprehensively improving the yield and economic benefits of artificially cultivated Dewgrass.

[0005] To achieve the above objectives, the technical solution of the present invention is implemented through the following technical solution:

[0006] A method for artificially cultivating and managing Dewgrass, the method comprising the following steps:

[0007] S1. Seed pretreatment: After disinfecting mature dewgrass seeds, fumigate them in an environment with an α-terpineol content of 0.2-0.5 g / L for 1-2 hours to obtain pretreated seeds for later use.

[0008] S2. Seed sowing treatment: After disinfecting the planting soil, mix it with base fertilizer, then sow the pretreated seeds and water thoroughly.

[0009] S3. Preparation of pea vine extract: After cutting pea vines into sections, mix them with 1%-1.5% saline solution and treat them at 50-60℃ for 15-20 minutes. Then filter the mixture, add water to the pea vines, grind and filter to obtain pea vine extract.

[0010] S4. Seedling Management: After sowing and seedling emergence, thoroughly water the seedlings once with a fertilizer solution containing 100-200 mg / L pea vine extract and 10-15 g / L urea.

[0011] S5, transplant management: after fertilizing the transplant field, plowing, then transplanting the dew grass seedlings with 5-6 leaves to the field, using the pea vine extract with a concentration of 100-200 mg / L as the rooting water to irrigate thoroughly;

[0012] S6, fertilization management: conventional fertilization treatment, and using the pea vine extract with a concentration of 100-200 mg / L to supplement irrigation once after transplanting for 2-3 months.

[0013] Preferably, the seed disinfection method in step S1 is to air dry for 2-3 days, then immerse in 3000-5000 times potassium permanganate solution or 1000-5000 times benomyl solution for 5-8 hours.

[0014] Preferably, the planting soil in step S2 is at least one of ordinary garden soil, red soil, brown soil or sandy soil, and the disinfection method is to spray the planting soil with 0.05%-0.1% potassium permanganate solution, then cover the film and stand for 1-2 days, then uncover the film and air dry for 5-7 days.

[0015] Preferably, the base fertilizer in step S2 is manure, and the mixing ratio of the planting soil and the base fertilizer is 4-6:1.

[0016] Preferably, the mass ratio of the pea vine to the water for grinding and filtering in step S3 is 1:3, and the grinding and filtering method is to grind and treat at a speed of 12000-18000 r / min for 5-10 minutes.

[0017] Preferably, the temperature during the seed sowing and germination is controlled to be 12-20℃.

[0018] Preferably, the timing of using the water and fertilizer in step S4 is after the germination rate reaches more than 50%.

[0019] Preferably, the amount of the field fertilization in step S5 is 1200-1500 kg / acre, and the fertilizer for the field fertilization is manure, superphosphate, wood ash and potassium sulfate with a mass ratio of 100-120:0.3-0.5:0.4-0.6:0.1-0.3.

[0020] Preferably, the conventional fertilization treatment method in step S6 is to use manure water + 10-15 g / L urea to supplement the fertilizer when transplanting for one month and three months.

[0021] The dew grass artificial planting management method provided by the application has the advantages of:

[0022] The present application effectively improves the growth efficiency of the early seedling of the Trifolium repens by early fumigation of the seed with alpha-terpineol and subsequent auxiliary treatment of the seedling and transplanting of the Trifolium repens with the extract of the pea vine, further promotes the growth and expansion of the root of the Trifolium repens and the accumulation of the ecdysone, comprehensively improves the yield and quality of the artificial planting of the Trifolium repens, increases the economic benefit of the planting, is suitable for the large-scale artificial planting, and guarantees the overall planting quality. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is a schematic view of the planting soil filled in the seedling tray in the embodiment of the present application.

[0024] Figure 2 It is a schematic view of the growth of the Trifolium repens in each experimental group 70 days after sowing in the embodiment of the present application.

[0025] Figure 3 It is a schematic view of the flowering of the Trifolium repens in the experimental group 1 after transplanting in the embodiment of the present application.

[0026] Figure 4 It is a comparative schematic view of the root of the Trifolium repens after harvesting in the experimental groups 1-3 in the embodiment of the present application.

[0027] Figure 5 It is a comparative schematic view of the root of the Trifolium repens after harvesting in the experimental groups 4-6 in the embodiment of the present application.

[0028] Figure 6 It is a comparative schematic view of the root of the Trifolium repens after harvesting in the experimental groups 7-8 in the embodiment of the present application. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme of the embodiments of the present application is described clearly and completely below in combination with the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the present application.

[0030] Embodiment:

[0031] Artificial planting experiment of the Trifolium repens:

[0032] 1. Seed pretreatment:

[0033] Select the same batch of mature Trifolium repens seeds, dry them for 3 days, then immerse them in the 5000 times liquid of the chlorothalonil for 8 hours to obtain the pretreated seeds SD-1.

[0034] Take part of the SD-1 seeds to the environment with the content of alpha-terpineol being 0.2g / L for fumigation treatment for 2 hours to obtain the pretreated seeds SD-2.

[0035] Take part of the SD-1 seed to fumigation treatment in the environment with α-terpineol content of 0.5 g / L for 1 h, and obtain pretreated seed SD-3;

[0036] Take part of the SD-1 seed to fumigation treatment in the environment with α-terpineol content of 1 g / L for 1 h, and obtain pretreated seed SD-4;

[0037] 2, preparation of extract of Pueraria montana var. lobata:

[0038] (1) After cutting Pueraria montana var. lobata into segments, it is treated in 1.5% salt water at 60°C water bath temperature for 15 min, then filtered. The filter residue is added with 3 times mass of clean water, and grinded by a grinder at a speed of 15000 r / min for 10 min, then pressed and filtered. The filtrate is dried at 40°C constant temperature to obtain PE-1;

[0039] (2) Pueraria montana var. lobata is added with 3 times mass of clean water, and grinded by a grinder at a speed of 15000 r / min for 10 min, then pressed and filtered. The filtrate is dried at 40°C constant temperature to obtain PE-2;

[0040] 3, sowing treatment:

[0041] Select ordinary garden soil as planting soil, spray 0.08% potassium permanganate solution, then cover film and stand for 2 days, then uncover the film and air dry for 7 days. Then mix the sheep manure at a mass ratio of 5:1, then fill the mixed sheep manure into 4x8 grid seedling trays (as shown in Figure 1 Control the temperature of the greenhouse at 12-20°C (24h temperature difference ≤5°C);

[0042] Sow the pretreated seeds into the seedling trays at 2-3 seeds per grid, then cover a layer of planting soil, and pour clear water. After 15 days, irrigate water and fertilizer once (the emergence rate is above 50%);

[0043] 4, transplanting management:

[0044] Set up 8 planting areas in Xiping County, Yunnan. After plowing, apply base fertilizer (sheep manure, superphosphate, wood ash, potassium sulfate at a mass ratio of 120:0.4:0.5:0.1) at 1500 kg / mu. Then transplant the 75d seedlings (grown to 5-6 leaves) to each planting area on April 10, 2024, and pour root-fixing water;

[0045] After one month of transplanting, apply the first supplementary fertilizer at 600 kg / mu. After three months of transplanting, apply the second supplementary fertilizer at 600 kg / mu.

[0046] Referring to the above artificial planting method, set up experimental groups 1-8 as shown in Table 1:

[0047] Table 1

[0048]

[0049] The fecal water in the above table is sheep fecal water with a mass concentration of 15%.

[0050] 5. Experimental results verification:

[0051] (1) The growth of the 70d seedlings of the experimental groups is as shown in Table 2 below: Figure 2 The height of each group of 10 randomly selected seedlings was measured (the height of the longest leaf of the aboveground part), and the average value was calculated. The specific results are shown in Table 2 below:

[0052] Table 2

[0053]

[0054] From the above Table 2 and Figure 2 It can be seen that the seedlings of experimental group 1, experimental group 2, experimental group 3, experimental group 5, experimental group 6, and experimental group 7 have good growth effects, while the seeds in experimental group 4 are not treated with α-terpineol fumigation and the subsequent water and fertilizer do not add PE-1 (equivalent to a blank control group), the overall seedling growth effect is poor, that is, α-terpineol fumigation and the addition of PE-1 can improve the growth of seedlings to a certain extent, while the addition of PE-2 in experimental group 8, the overall growth of seedlings is significantly lower than that of experimental group 4, and in Figure 2 some seedlings appear yellowing and wilting, which indicates that the PE-2 obtained by not soaking the extract of the pea vine in salt water has a certain inhibitory effect on the growth of the seedlings of the grass.

[0055] (2) Each group of grasses began to bloom about 3 months after transplanting Figure 3 The flowering of experimental group 1 after transplanting is shown), and on November 15, 2024, the grasses in each group were harvested, the upper stems and leaves were removed, the roots were retained, and the soil around the roots was removed as much as possible. 10 plants were randomly selected from each group, and the number of roots, root length (measured by the longest root of a single plant), and root weight (fresh weight) were counted (averaged), and the final results are shown in Table 3 below:

[0056] Table 3

[0057]

[0058] From the above Table 3 and Figures 4-6As shown, the seeds in experimental group 4 were not fumigated, and the subsequent water and fertilizer and fertilizer were not added to the extract of pea vine, which is equivalent to the blank control group. The root growth of experimental group 1 and experimental group 2 was significantly improved in root number, root length and root weight compared with experimental group 4. The fumigation of seeds in experimental group 1 and experimental group 2 and the use of PE-1 in subsequent stages can significantly improve the growth of roots. Although experimental group 3 has progress in root growth compared with experimental group 4, there is a large difference in root number and root weight compared with experimental group 1 and experimental group 2. The use of high concentration of a-pinene fumigation seed (1g / L) in experimental group 3 is not as good as the effect of 0.2-0.5g / L concentration fumigation in experimental group 1-2. In experimental group 5, low concentration (50mg / L) of PE-1 is used to assist planting, and finally the root growth of experimental group 4 is not much different, that is, low concentration of PE-1 has little effect on the root growth of experimental group 4. In experimental group 6, high concentration (300mg / L) of PE-1 is used to assist planting, and finally the root growth effect is significantly higher than that of experimental group 4, but still lower than that of experimental group 1-2. The seeds in experimental group 7 are not fumigated, and PE-1 is used in subsequent planting. The overall root growth is significantly higher than that of experimental group 4, but slightly lower than that of experimental group 1-2. Although the seeds in experimental group 8 are fumigated, PE-2 (extract of pea vine prepared without salt water treatment) is used in subsequent planting. The overall root growth effect is significantly lower than that of experimental group 4, that is, PE-2 has a certain inhibitory effect on the root growth of experimental group 4.

[0059] (3) The roots of experimental group 1-8 were washed and placed in a hot air drying machine at 50°C, and dried to a water content of ≤12%. The β-ecdysone content in the dried roots of each group was detected, as shown in Table 4:

[0060] Table 4

[0061]

[0062] From the above table 4, it can be seen that the content of β-ecdysone in the dry weight of the roots of the experimental group 4 (equivalent to the control group) is 3.24%, while the content of β-ecdysone in the experimental groups 1 and 2 is more than 5.5%, which has a significant progress compared with the experimental group 4; while in the experimental group 3, the seeds are fumigated with high concentration of α-terpineol (1 g / L), and the final content of β-ecdysone is higher than that of the experimental group 4, but significantly lower than that of the experimental groups 1-2; in the experimental groups 5-6, PE-1 is used for treatment at low and high concentrations respectively, but the final content of β-ecdysone has little difference compared with the experimental groups 1-2; in the experimental group 7, the seeds are not fumigated, and the final content of β-ecdysone has little difference compared with the experimental group 4; while in the experimental group 8, the seeds are fumigated, and although PE-2 is used for treatment subsequently, the overall content of β-ecdysone still has a certain improvement compared with the experimental group 4.

[0063] In summary, in the present application, the seeds are fumigated with a certain concentration of α-terpineol, and the pea vine extract prepared after the subsequent salt water treatment can significantly improve the root growth effect of the droplet grass, increase the yield and the content of β-ecdysone in the roots, and comprehensively improve the economic benefit of planting.

[0064] The above examples are only used to illustrate the technical solutions of the present application, but not to limit it; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that the technical solutions recorded in the foregoing examples can be modified, or some technical features can be replaced by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for promoting the growth and swelling of the root of dew grass and the accumulation of ecdysone in artificial planting management, characterized by, The artificial planting management method comprises the following steps: S1, seed pretreatment: after disinfecting mature dew grass seeds, fumigation treatment is carried out in an environment with an α-terpineol content of 0.2-0.5 g / L for 1-2 h, and the pretreated seeds are obtained for standby; S2, seed sowing treatment: after disinfecting the planting soil and adding base fertilizer, the pretreated seeds are sown and then watered with clear water; S3, preparation of pea vine extract: after cutting the pea vine and mixing with salt water with a concentration of 1%-1.5% at a temperature of 50-60 ℃ for 15-20 min, filtration is carried out, then the pea vine is added to clear water for grinding and filtration, and the filtrate is dried to obtain the pea vine extract; the mass ratio of the pea vine added to clear water for grinding and filtration is 1:3, and the grinding and filtration is carried out at a speed of 12000-18000 r / min for 5-10 min; S4, seedling management: after sowing and germination, water and fertilizer with a formula of pea vine extract 100-200 mg / L+urea 10-15 g / L are used for watering once; S5, transplanting management: after fertilizing and plowing the transplanting field, dew grass seedlings growing to 5-6 leaves are transplanted into the field, and pea vine extract with a concentration of 100-200 mg / L is used as rooting water for watering; S6, fertilization management: conventional fertilization treatment is carried out, and pea vine extract with a concentration of 100-200 mg / L is used for supplemental irrigation once after 2-3 months of transplanting.

2. The artificial planting management method according to claim 1, characterized in that: In step S1, the dew grass seeds are first dried for 2-3 days, then soaked in a potassium permanganate solution with a concentration of 3000-5000 times or a chlorothalonil solution with a concentration of 1000-5000 times for 5-8 h.

3. The artificial planting management method according to claim 1, characterized in that: In step S2, the planting soil is selected from at least one of ordinary garden soil, red soil, brown soil or sandy soil, and the disinfection method is to spray the planting soil with a potassium permanganate solution with a concentration of 0.05%-0.1%, then cover the film and stand for 1-2 days, then uncover the film and dry naturally for 5-7 days.

4. The artificial planting management method according to claim 1, characterized in that: The base fertilizer in step S2 is manure, and the mixing ratio of the planting soil and the base fertilizer is 4-6:

1.

5. The artificial planting management method according to claim 1, characterized in that: The temperature of the seeds during sowing and germination is controlled at 12-20 ℃.

6. The artificial planting management method according to claim 1, characterized in that: In step S4, the water and fertilizer are used after the germination rate reaches more than 50%.

7. The artificial planting management method according to claim 1, characterized in that: In step S5, the amount of field fertilization is 1200-1500 kg / acre, and the fertilizer for field fertilization is a mixture of manure, superphosphate, wood ash, potassium sulfate with a mass ratio of 100-120:0.3-0.5:0.4-0.6:0.1-0.

3.

8. The artificial planting management method according to claim 1, characterized in that: In step S6, the conventional fertilization treatment is to use manure water+10-15 g / L urea for supplemental fertilization at one month and three months after transplanting.

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