Acaricidal composition

Abstract

The invention belongs to the technical field of pesticides, and relates to an acaricidal composition. The effective components of the acaricidal composition comprise an active component A and an active component B, the active ingredient A is flufenacet thioether, and the active ingredient B is selected from Acynonapyr. The acaricidal composition or the preparation thereof can enhance the pesticide effect, enlarge the acaricidal spectrum, reduce the use cost, reduce the dosage, prolong the lasting period and delay the development of pesticide resistance.

Description

Technical Field

[0001] The invention relates to the technical field of pesticide compounding, in particular to an acaricidal composition. The acaricidal composition or its preparation can enhance drug efficacy, reduce drug dosage, and at the same time improve quick-acting property, prolong the duration of effect and delay the development of drug resistance. Background Art

[0002] In the process of agricultural production, pests and diseases have always been one of the main disasters. According to incomplete statistics, the world's grain and cotton production loses more than 10% every year due to pests and diseases. These diseases not only cause a reduction in crop yields, but also pose a threat to the quality and safety of crop products and their international trade to a certain extent. Among them, herbivorous spider mites have seriously threatened the quality and safety of agricultural products, causing a lot of losses to farmers.

[0003] Fluomethiocarb is chemically named (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide, and its chemical structure is as follows:

[0004]

[0005] Acynonapyr is a novel miticide with a cyclic amine skeleton developed by Japan Soda Co., Ltd. It acts on inhibitory glutamate receptors (IGluRs), interferes with the neurotransmission of mites, causes muscle cells to lose their original motor ability, and exhibits a state similar to paralysis, which in turn affects eating or movement, and ultimately kills mites. It has a good preventive effect on mites in fruit trees, vegetables and tea trees.

[0006] In the agricultural production process, chemical agents are the most effective means of preventing and controlling pests and diseases. Long-term continuous high-dose use of a single chemical agent can easily cause a series of problems such as resistance of mites and environmental pollution. Reasonable compounding or mixing of chemical agents has the positive characteristics of expanding the insecticide spectrum, improving the control effect, extending the appropriate period of application, reducing the amount of medicine used, reducing drug damage, reducing residues, and delaying the occurrence of pest resistance and resistance. The applicant was surprised to find through indoor toxicity tests and field efficacy tests that the compound combination of fluazifop and Acynonapyr has a significant synergistic effect, and the acaricide composition and application of fluazifop and Acynonapyr have not been reported yet. Summary of the invention

[0007] Based on the above situation, the purpose of the present invention is to provide a miticide composition and its preparation, which are mainly used for controlling herbivorous mites. The miticide composition or its preparation can enhance the efficacy, reduce the dosage, and at the same time can prolong the lasting effect and delay the development of drug resistance.

[0008] In order to achieve the above object, the present invention provides the following technical solution: a miticide composition comprising an active ingredient A, thiofluanid, and an active ingredient B, aceynonapyr;

[0009] Furthermore, the mass ratio of fluazifop to aceynonapyr is 1:30 to 35:1;

[0010] Furthermore, the mass ratio of fluazifop to aceynonapyr is 1:20 to 25:1;

[0011] Furthermore, the mass ratio of fluazifop to Acynonapyr is 1:30 to 35:1;

[0012] Furthermore, the mass ratio of fluazifop to acynomolgus pyr is 1:30, 1:20, 1:15, 2:15, 1:3, 3:4, 15:2, 15:1, 25:1, 35:1;

[0013] Furthermore, the mass ratio of fluazifop to aceynonapyr is 1:20 to 25:1;

[0014] Furthermore, the mass ratio of fluazifop to aceynonapyr is 1:20, 1:15, 2:15, 1:3, 3:4, 15:2, 15:1, 25:1;

[0015] Furthermore, the mass ratio of fluazifop to aceynonapyr is 1:20, 1:12, 2:17, 1:6, 1:2, 8:1, 12:1, 25:1;

[0016] Furthermore, the mass ratio of fluazifop to acynonapyr is 2:17 to 12:1;

[0017] Furthermore, the mass ratio of fluazifop to acynonapyr is 2:17, 1:6, 1:2, 8:1, and 12:1.

[0018] A mite-killing composition containing fluazifop-sulphonate and acynonapyr, wherein the total weight of the mite-killing composition is 100wt%, the sum of the contents of the active ingredient A and the active ingredient B in the mite-killing composition is 1-95wt%,

[0019] Furthermore, based on the total mass of the acaricidal composition being 100 wt%, the sum of the contents of fluazifop and aceynonapyr in the acaricidal composition is 5 to 50 wt%;

[0020] Furthermore, based on the total mass of the acaricidal composition being 100wt%, the sum of the contents of fluazifop and acynonapyr in the acaricidal composition is 10wt%, 15wt%, 20wt%, 25wt%, 40wt%, and 45wt%;

[0021] Furthermore, the acaricide composition further comprises an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist and a carrier;

[0022] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0023] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0024] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0025] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0026] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0027] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0028] The defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C 8 -C10 One or more of fatty alcohols; and / or

[0029] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0030] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0031] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0032] The synergist is selected from synergist phosphite, synergist ether; and / or

[0033] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0034] The invention optimizes the content of active ingredients and auxiliary agents in the acaricide composition to achieve a better balance between toxicity and residue, thereby enhancing the efficacy, reducing the dosage and lowering the cost.

[0035] Furthermore, the pesticide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation;

[0036] Furthermore, the solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions.

[0037] The specific implementation scheme of the pesticide formulation prepared by the acaricide composition of the present invention is as follows:

[0038] When the acaricide composition is a wettable powder, the mass proportions of the components are: 1 to 40 parts of fluazifop-sulfuron; 1 to 40 parts of acynonapyr; 3 to 10 parts of dispersant; 1 to 5 parts of wetting agent; and the balance is made up of filler.

[0039] The specific production steps of the wettable powder are as follows: according to the above formula, the active ingredients, dispersant, wetting agent and filler are mixed, stirred evenly in a mixer, and mixed evenly after air flow crushing to prepare the wettable powder of the composition of the present invention.

[0040] When the acaricide composition is a water-dispersible granule, the components are as follows by weight: 1-40 parts of fluazifop-sulfuron; 1-40 parts of acynonapyr; 3-10 parts of dispersant; 1-10 parts of wetting agent; 1-5 parts of disintegrant; and the balance is made up of fillers.

[0041] The specific production steps of the water dispersible granules are as follows: according to the above formula, the active ingredients and the dispersant, the wetting agent, the disintegrant and the filler are uniformly mixed, crushed with an ultra-fine airflow pulverizer, kneaded, and then added into a fluidized bed granulation dryer for granulation, drying, sieving and sampling and analysis, thereby preparing the water dispersible granules of the composition of the present invention.

[0042] When the acaricide composition is an emulsifiable concentrate preparation, the mass proportions of the components are: 1 to 40 parts of fluazifop; 1 to 40 parts of acynonapyr; 10 to 30 parts of a conventional emulsifier; 20 to 50 parts of a conventional solvent; and 1 to 5 parts of a conventional synergist.

[0043] The specific production steps of the emulsifiable concentrate preparation are: firstly, the effective ingredients are added into a solvent and completely dissolved, then an emulsifier and a synergist are added and stirred evenly to form a uniform and transparent oily liquid, and then the emulsifiable concentrate preparation of the composition of the present invention is prepared by filling.

[0044] When the acaricide composition is an aqueous emulsion, the components are as follows by weight: 1 to 40 parts of fluazifop-sulfuron; 1 to 40 parts of acynomolgus; 3 to 30 parts of emulsifier; 5 to 15 parts of solvent; 2 to 15 parts of stabilizer; 1 to 5 parts of antifreeze; 0.1 to 8 parts of defoamer; 0.2 to 2 parts of thickener; and the balance is made up of deionized water.

[0045] The specific production steps of the aqueous emulsion are: firstly, the active ingredient, solvent, emulsifier and cosolvent are added together to dissolve into a uniform oil phase; deionized water, antifreeze agent, antimicrobial agent and other pesticide adjuvants are mixed together to form a uniform water phase; while stirring at high speed in a reaction kettle, the oil phase is added into the water phase, and a shearing machine is turned on for high-speed shearing for about half an hour to form an oil-in-water aqueous emulsion, thereby preparing the aqueous emulsion of the composition of the present invention.

[0046] When the acaricide composition is a microemulsion, the mass parts of the components are: 1-40 parts of fluazifop-butyl; 1-40 parts of acynonapyr; 10-30 parts of emulsifier; 1-8 parts of antifreeze; 0.5-10 parts of stabilizer; and 20-50 parts of conventional solvent cosolvent.

[0047] The specific production steps of the microemulsion are: completely dissolving the active ingredient with a cosolvent, then adding other ingredients such as an emulsifier, an antifreeze stabilizer, etc., mixing them evenly, and finally adding water, and stirring them fully to prepare the microemulsion.

[0048] When the acaricide composition is a suspension, the components are as follows in parts by weight: 1 to 40 parts of fluazifop-sulfuron; 1 to 40 parts of acynonapyr; 1 to 10 parts of a wetting and dispersing agent; 1 to 5 parts of an antifreeze agent; 0.1 to 2 parts of a thickener; 0.1 to 0.8 parts of a defoaming agent; 0 to 10 parts of a solvent; 1 to 5 parts of a stabilizer; and the balance is made up of deionized water.

[0049] The specific production steps of the suspension are: using zirconium beads, wet grinding with a sand mill until D90 (90% of the particle size) is less than 5 μm, and the suspension preparation of the composition of the present invention can be prepared.

[0050] When the insecticide composition is a suspoemulsion, the components are as follows in parts by weight: 1 to 40 parts of fluazifop-sulfuron; 1 to 40 parts of acynomolgus; 1 to 10 parts of a wetting and dispersing agent; 1 to 5 parts of an antifreeze agent; 0.1 to 2 parts of a thickener; 0.1 to 0.8 parts of a defoaming agent; 1 to 40 parts of a solvent; 1 to 5 parts of a stabilizer; and the balance is made up of deionized water.

[0051] The specific production steps of the suspoemulsion are: Acynonapyr is completely dissolved with a solvent to prepare an oil phase; fluazifop, a wetting dispersant, an antifreeze agent, a defoamer, deionized water, etc. are wet-grinded to a suspension mother liquor with a D90 less than 5 μm; the oil phase is added to the suspension mother liquor and stirred evenly, and then a thickener is added and stirred and sheared evenly to prepare a suspoemulsion preparation of the composition of the present invention.

[0052] When the insecticide composition is a dispersible oil suspension, the components are as follows in parts by weight: 1 to 40 parts of fluazifop-sulfuron; 1 to 40 parts of acynonapyr; 1 to 10 parts of a wetting and dispersing agent; 0.1 to 2 parts of a thickener; 0.1 to 0.8 parts of a defoaming agent; 0 to 10 parts of a solvent; 1 to 5 parts of a stabilizer; and the balance is made up of vegetable oil.

[0053] The specific production steps of the dispersible oil suspension are: using zirconium beads, wet grinding with a sand mill until D90 (90% of the particle size) is less than 5 μm, and the dispersible oil suspension preparation of the composition of the present invention can be prepared.

[0054] Application of the acaricidal composition of the present invention in preventing and controlling harmful mites;

[0055] Furthermore, the harmful mites are herbivorous harmful mites;

[0056] Furthermore, the herbivorous mites are Tetranychus cinnabarinus, Tetranychus truncatus, Tetranychus two-spotted, and Citrus red spider;

[0057] Furthermore, the acaricidal composition and / or its preparation is applied at an effective dose to the harmful mites to be controlled and / or the medium where they grow.

[0058] Compared with the prior art, the beneficial effects of the technical solution of the present invention are as follows:

[0059] (1) The compound acaricide of the present invention has a significant synergistic effect compared with a single agent, thereby improving the control effect;

[0060] (2) The compound acaricide of the present invention can improve the control effect while reducing the dosage, cost and residue;

[0061] (3) The compound acaricide of the present invention is compounded from two agents with different action mechanisms, has no cross-resistance, and is beneficial for delaying the development of drug resistance in mites. DETAILED DESCRIPTION

[0062] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0063] Indoor toxicity assay

[0064] Test insect source: citrus red spider (raised in the R&D center);

[0065] Preparation of test materials: Select mites (adult female mites or nymphs) raised indoors and in the same physiological state. Cut the double-sided tape into 2 cm long pieces and stick them on one end of the slide. Then select healthy mites and stick their backs on the double-sided tape. Put 30 mites per slide into a container padded with a wet sponge, cover it with a lid, and place it at (25±1)℃. After 2 hours, examine under a microscope, remove dead and injured individuals, and make up 30 mites per slide.

[0066] Preparation of drugs: The original drug was prepared into a mother solution with acetone, and the mother solution was prepared into test drugs with different proportions using 0.1% Tween-80 aqueous solution. The specific proportions and concentration gradients are as follows:

[0067] Table 1 Concentration gradients of flubendiamide and Acynonapyr alone and in combination

[0068] Serial number Test treatment Dilution concentration (mg / L) 1 Fluometra 0.75、1.5、3、6、12 2 Acynonapyr 0.5、1、2、4、8 3 Flumethoxane:Acynonapyr=1:30 0.5、1、2、4、8 4 Flumethoxane:Acynonapyr=1:20 0.5、1、2、4、8、16 5 Flumethoxane:Acynonapyr=1:15 0.5、1、2、4、8 6 Flumethoxane:Acynonapyr=1:12 1、2、4、8、16 7 Flumethoxane:Acynonapyr=2:17 1、2、4、8、16 8 Flumethoxane:Acynonapyr=2:15 0.5、1、2、4、8 9 Flumethoxane:Acynonapyr=1:6 0.5、1、2、4、8 10 Flumethoxane:Acynonapyr=1:3 0.5、1、2、4、8 11 Flumethoxane:Acynonapyr=1:2 0.5、1、2、4、8 12 Flumethoxane:Acynonapyr=3:4 0.25、0.5、1、2、4 13 Flumethoxane:Acynonapyr=15:2 0.25、0.5、1、2、4 14 Flumethoxane:Acynonapyr=8:1 0.5、1、2、4、8 15 Flumethoxane:Acynonapyr=12:1 0.5、1、2、4、8 16 Flumethoxane:Acynonapyr=15:1 0.25、0.5、1、2、4 17 Flumethoxane:Acynonapyr=25:1 0.5、1、2、4、8、16 18 Flumethoxane:Acynonapyr=35:1 0.5、1、2、4、8

[0069] Drug treatment: Immerse the slide in the drug solution and shake it gently for 5 seconds, then take it out, absorb the excess drug solution with absorbent paper, place it in a white porcelain plate with a wet sponge, cover it with a plastic film with good light transmittance, and place it at (25±1)℃ for observation. Each treatment is repeated 4 times, and a treatment without drug is set as a blank control.

[0070] Test investigation: 48 hours after treatment, check the death of test insects, record the total number of insects and the number of dead insects, and calculate the mortality rate:

[0071]

[0072] Where:

[0073] P——mortality rate, in percentage (%);

[0074] K——indicates the number of dead insects, in units of heads;

[0075] N——represents the total number of insects treated, in heads.

[0076]

[0077] Where:

[0078] P 1 ——Adjusted mortality rate, expressed as percentage (%);

[0079] P t ——Treatment mortality rate, expressed as percentage (%);

[0080] P 0 ——Blank control mortality rate, in percentage (%).

[0081] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0082] The LC of each drug was calculated by linear regression analysis between the probability value of mortality and the logarithmic value of the series concentration. 50 The Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC) of the mixture to evaluate the activity of the test agent against the test insects; the specific calculation formula is as follows:

[0083]

[0084] Where:

[0085] ATI——Actual Toxicity Index of Mixture;

[0086] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0087] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0088] TTI=TI A *P A +TI B *PB

[0089] Where:

[0090] TTI – Theoretical Toxicity Index of Mixtures;

[0091] TI A ——A: toxicity index of the agent;

[0092] P A ——The percentage of agent A in the mixture, in percentage (%);

[0093] TI B ——Toxicity index of agent B;

[0094] P B ——The percentage of agent B in the mixture, in percentage (%).

[0095]

[0096] Where:

[0097] CTC – Co-toxicity coefficient;

[0098] ATI——Actual Toxicity Index of Mixture;

[0099] TTI - Theoretical Toxicity Index of Mixture.

[0100] According to the classification standard of combined effects: a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect, a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect, and a co-toxicity coefficient (CTC) 80 < CTC < 120 indicates an additive effect.

[0101] Test results:

[0102] The combined toxicity test of flumethoxetine and Acynonapyr in different ratios against citrus red spider mites (female adult mites) is shown in Table 2. 50 The value is 3.518mg / L, and the LC value of Acynonapyr to citrus spider mite 50 The value is 1.963mg / L.

[0103] There was no antagonistic effect between flumethoxetine and Acynonapyr in the range of 1:30-35:1 on the control of citrus red spider mites. Flumethoxetine and Acynonapyr showed synergistic effect in the range of 1:20-25:1 on the control of citrus red spider mites. When flumethoxetine and Acynonapyr were 3:4, LC 50 The value was 1.068 mg / L, and the co-toxicity coefficient was the highest, which was 226.705.

[0104] Table 2 Indoor activity test results of fluazifop-sulphonate and Acynonapyr against citrus red spider mites (female adult mites)

[0105]

[0106] The combined toxicity test of flumethoxetine and Acynonapyr in different ratios against citrus red spider (nymph) is shown in Table 3. 50 The value is 3.971mg / L, and the LC value of Acynonapyr to citrus spider mite 50 The value was 5.428 mg / L. Flumethoxam and Acynonapyr showed synergistic effect on the control of citrus red spider mites in the range of 1:20-25:1. When flumethoxam and Acynonapyr = 1:2, the co-toxicity coefficient was the highest, which was 232.117, and LC 50 The value is 2.083mg / L.

[0107] Table 3 Indoor activity test results of fluazifop-sulphonate and Acynonapyr against citrus red spider mites (nymphs)

[0108]

[0109] Formulation Examples

[0110] Preparation Example 1: 21% thiofluanidazole·Acynonapyr suspension (9+12)

[0111] Preparation formula: flumethoxane 9%, Acynonapyr 12%, sodium dioctyl sulfosuccinate 2%, EO-PO block copolymer 2%, styrylphenol polyoxyethylene ether phosphate 3%, polycarboxylic acid sodium salt 1%, silicone defoamer 0.5%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 5%, sodium benzoate 0.5%, deionized water is added to 100%;

[0112] Preparation method: Add flumethoxane and Acynonapyr to the wetting and dispersing agent and defoaming agent, stir evenly, and use zirconium beads and a sand mill to wet grind to D 90 (90% of the particle size) <5 μm, to obtain a crushed slurry; add a thickener, an antifreeze agent, and a preservative to the crushed slurry and mix them evenly, add deionized water to 100%, and high-speed shear to obtain a suspension. Preparation Example 2: 24% thiofluanid·Acynonapyr suspension (8+16)

[0113] Preparation formula: fluazifop 8%, acynoonapyr 16%, alkyl naphthalene sulfonate formaldehyde condensate 3%, sodium lignin sulfonate 2%, EO-PO block copolymer 1%, sodium dodecyl sulfate 2%, organosilicon defoamer 0.5%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 3%, glycerol 2%, sodium benzoate 0.5%, deionized water is added to 100%;

[0114] Preparation method: Add flumethoxetine and Acynonapyr to the wetting dispersant and defoamer, stir evenly, use zirconium beads, and use a sand mill to wet grind until D90 (90% of the particle size) is less than 5μm to obtain a crushed slurry; add a thickener, antifreeze agent, and preservative to the crushed slurry and mix evenly, add deionized water to 100%, and high-speed shear to obtain a suspension. Preparation Example 3: 40% flumethoxetine·Acynonapyr wettable powder (10+30)

[0115] Preparation formula: Fluomethiocarb 10%, Acynonapyr 30%, Sodium lignin sulfonate 15%, Sodium lauryl sulfate 3%, Bentonite 6%, Kaolin added to 100%;

[0116] Preparation method: pre-crush and mix evenly, then grind by air flow mill to a fineness that satisfies the requirement that at least 98wt% passes through a 45μm test sieve to obtain a wettable powder.

[0117] Preparation Example 4: 45% fluazifop-Acynonapyr wettable powder (20+25)

[0118] Preparation formula: Fluomethiocarb 20%, Acynonapyr 25%, Sodium lignin sulfonate 12%, Sodium lauryl sulfate 3%, Starch 10%, Kaolin added to 100%;

[0119] Preparation method: pre-crush and mix evenly, then grind by air flow mill to a fineness that satisfies the requirement that at least 98wt% passes through a 45μm test sieve to obtain a wettable powder.

[0120] Preparation Example 5: 32% thiofluanid · Acynonapyr water dispersible granules (16+16)

[0121] Preparation formula: fluazifop 16%, acynoonapyr 16%, lignin sulfonate 6%, naphthalene sulfonate formaldehyde condensate 10%, α-olefin sulfonate 2%, ammonium sulfate 2%, kaolin 20%, bentonite added to 100%;

[0122] Preparation method: first mix evenly, then grind to less than 15μm by air flow mill, mix evenly with water, granulate by rotary granulator, dry at 60℃ to a moisture content of ≤3%, select 20-mesh and 60-mesh test sieves for sieving, discard the large particles on the top and the dust filtered at the bottom, and prepare water-dispersible granules.

[0123] Preparation Example 6: 8% thiofluanidazole·Acynonapyr emulsifiable concentrate (5:3)

[0124] Preparation formula: flumethoxane 5%, Acynonapyr 3%, DMF 15%, cyclohexanone 15%, calcium dodecylbenzene sulfonate 3%, styrylphenol polyoxyethylene ether 12%, solvent oil to 100%;

[0125] Preparation method: Add the active ingredients into the co-solvent according to the formula ratio of the embodiment, and add the surfactant and other functional additives thereto, stir and mix them evenly in a stirring mixing kettle to obtain an emulsifiable concentrate product.

[0126] Preparation Example 7: 15% thiofluanidine·Acynonapyr suspoemulsion (10:5)

[0127] Preparation formula: flumet thioether 10%, Acynonapyr 5%, cyclohexanone 20%, sodium lignin sulfonate 2%, fatty amine polyoxyethylene ether 3%, polycarboxylate 2%, naphthalene sulfonate 5%, sodium p-hydroxybenzoate 0.5%, xanthan gum 0.2%, magnesium aluminum silicate 1%, propylene glycol 3.5%, glycerol 0.5%, silicone defoamer 0.2%, deionized water to 100%;

[0128] Preparation method: According to the formula ratio of the embodiment, Acynonapyr is completely dissolved in a solvent to prepare an oil phase; fluazifopylene sulfide, a wetting dispersant, an antifreeze agent, a defoaming agent, deionized water, etc. are wet-grinded to D 90 The suspension mother liquid is prepared by mixing the oil phase into the suspension mother liquid and stirring evenly, and then adding the thickener and stirring and shearing evenly to prepare the suspoemulsion preparation of the composition of the present invention.

[0129] Preparation Example 8: 14% fluazifop-Acynonapyr dispersible oil suspension (8:6)

[0130] Preparation formula: flumethoxane 8%, Acynonapyr 6%, sodium diethyl sulfonate succinate 2%, fatty amine polyoxyethylene ether 3%, castor oil polyoxyethylene ether 13%, calcium dodecylbenzene sulfonate 3%, organic bentonite 1%, corn oil to 100%;

[0131] Preparation method: according to the formula ratio of the embodiment, the active ingredients, surfactants and other functional additives are placed in a reaction kettle in sequence, solvent oil is added and mixed evenly, and the dispersible oil suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0132] Field trials

[0133] Field trials to control citrus red spider mites

[0134] Tested crops: citrus;

[0135] Test subjects: Citrus spider mite;

[0136] Trial time: late April 2022;

[0137] Experimental location: Nanfeng, Jiangxi;

[0138] Pest occurrence: Citrus red spider mites are quite harmful;

[0139] Experimental field: The soil fertility of the experimental field is moderate, the terrain is flat, the fertility is uniform, and the irrigation conditions are good. The cultivation conditions (soil type, fertilization, tillage, row spacing, etc.) of all experimental plots are uniform and consistent with the local agricultural cultivation practice (GAP).

[0140] Experimental design: The preparation treatment, control treatment and blank control were arranged in random blocks, with a plot area of ​​2 citrus trees and each treatment repeated 4 times.

[0141] Table 4 Experimental treatment and dosage

[0142] Processing number Pharmacy <![CDATA[Application rate of active ingredient g a.i / hm 2 > Process 1, 2 24% Fluomethiocarb·Acynonapyr Suspension 30、60 Process 3, 4 45% Cypermethrin·Acynonapyr WP 30、60 Process 5, 6 32% Cyproconazole Acynonapyr water dispersible granules 30、60 Comparison 1 20% Acynonapyr suspension 60 Comparison 2 20% sulfamethoxazole suspension 60 Comparison 3 Water blank control /

[0143] Spraying method: spray treatment, the spraying equipment is WS-16D Guardian electric sprayer, the nozzle is a single fan-shaped mist nozzle, the working pressure is 0.15-0.4Mpa, according to the dosage requirements of the pesticide and the area of ​​the plot, accurately measure the dosage.

[0144] The weather was good during the test. On the day of application, the weather was sunny with an average daily temperature of 22°C, a maximum temperature of 27°C, a minimum temperature of 19°C, an east wind of level 1, and a relative humidity of 65%.

[0145] Survey method: mark the tender shoots in the east, west, south, north and center of the tree in each plot, hang 20 leaves on the trees for investigation, and the number of mites should be no less than 200 before treatment; investigate the number of live mites 3d and 14d after application.

[0146] Calculation method of drug efficacy:

[0147]

[0148]

[0149] During the experiment, citrus fruits in each treatment plot grew well and no pesticide damage was observed in any treatment.

[0150] Field test results:

[0151] Table 5 Field test results of different tested pesticides on citrus red spider mites

[0152]

[0153] Note: The protective effects (%) in the above table are the average values ​​of each replicate. Capital letters represent significant differences at the 1% level.

[0154] The results of the field test showed (Table 5) that different tested agents had good control effects on citrus red spider mites. 3 days after application, the control effect of the acaricide composition obtained by combining flumethoxetine and Acynonapyr on citrus red spider mites was more than 80%, and each combination showed good rapid effectiveness. 14 days after application, the control effect of the acaricide composition obtained by combining flumethoxetine and Acynonapyr on citrus red spider mites was more than 85%, and each combination showed good persistence. The test data were processed using the Duncan method using IBM SPSS data processing software. The results of variance analysis showed that there were significant differences between each combination group and the control group at 0.01.

[0155] According to the test results, in the initial stage of citrus red spider mites, the use of various compound miticides can effectively control its damage in a short period of time and reduce the economic losses caused by pests to citrus. It is recommended to spray evenly when applying the pesticide, with emphasis on the back of the leaves.

[0156] Through indoor toxicity determination and field tests on citrus, the acaricidal composition compounded with flumetofen and Acynonapyr of the present invention showed good control effect on phytophagous mites. The acaricidal composition or its preparation obtained by compounding of the present invention has significant control effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging the lasting effect. In addition, no phytotoxicity of the compound agent to crops was found in the test, indicating that when the synergistic effect of acaricidal mites of the obtained acaricidal composition or preparation is improved, the production cost and use cost can be reduced, and it is safe for crops.

[0157] The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different options and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A mite-killing composition, Features: Includes active ingredient A thiosulfate and active ingredient B cynonapyr.

2. The acaricidal composition according to claim 1, It is characterized in that The mass ratio of fluazifop to acynonapyr is 1:30 to 35:

1.

3. The acaricidal composition according to claim 2, It is characterized in that The mass ratio of fluazifop to acynonapyr is 1:20 to 25:

1.

4. The acaricidal composition according to claim 1, It is characterized in that Based on the total weight of the acaricidal composition being 100 wt %, the sum of the contents of the active ingredient A and the active ingredient B in the acaricidal composition is 1 to 95 wt %; Preferably, the total content of the active ingredient A and the active ingredient B in the acaricidal composition is 5 to 45 wt %.

5. The acaricidal composition according to claim 1, It is characterized in that The acaricide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

6. The acaricidal composition according to claim 5, It is characterized in that The acaricidal composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation.

7. The acaricidal composition according to claim 6, It is characterized in that The solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions.

8. Use of the acaricidal composition according to any one of claims 1 to 7 for controlling harmful mites.

9. The use according to claim 8, It is characterized in that The harmful mites are herbivorous harmful mites; Preferably, the phytophagous pest mites are Tetranychus cinnabarinus, Tetranychus truncatus, Tetranychus two-spotted, and Citrus red spider.

10. The use according to claim 8, It is characterized in that The acaricidal composition and / or its preparation is applied at an effective dose to the harmful mites to be controlled and / or the medium where they grow.

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