A pdx model tumor specimen implantation device

Abstract

The present application relates to specimen implantation device technical field, especially in a kind of PDX model tumor specimen implantation device;Operator first mouse is fixed on operation table, then the device is placed on operation table and close to mouse;Rotating implantation cylinder to implantation cylinder is aligned to the position of mouse to be implanted, the position and angle of implantation cylinder are then locked by first locking component, then implantation cylinder and piston rod are simultaneously pushed to embed sharp head into the position of mouse to be implanted;During pushing process, sharp head will pierce mouse skin to form wound, and simultaneously drive implantation cylinder to extend into mouse body;Piston rod is extracted, then sharp head on piston head is unscrewed, and plug screw is installed on piston head, then piston head is re-embedded into implantation cylinder, specimen is placed into implantation cylinder through inlet, and then piston rod is manually pushed, so as to implant specimen into mouse body;After implantation is completed, implantation cylinder is pulled out from mouse body;Whole operation process is safer, more convenient and efficient.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of specimen implantation devices, and particularly relates to a PDX model tumor specimen implantation device. BACKGROUND

[0002] Patient-derived xenograft (PDX) is an animal model that reappears the biological characteristics and microenvironment of a patient's tumor by directly transplanting the patient's tumor tissue into an immunodeficient mouse. Compared with other models (such as cells, organoids and cell-derived xenotransplantation animal models), the PDX model can highly retain the inherent characteristics of the patient's tumor, including tumor heterogeneity, genomic stability and clinical drug response correlation. At present, the PDX model is constructed by surgery in the industry, which requires a certain degree of incision of the mouse skin or peritoneum, and the specimen is implanted into the mouse body by hand-held forceps and then sutured. The whole process is relatively cumbersome, time-consuming and laborious, and has a large trauma, which is easy to cause the death of the mouse due to surgical complications during or after the operation. SUMMARY

[0003] The main purpose of the present application is to provide a PDX model tumor specimen implantation device, which aims to solve the problem that the current process of constructing a PDX model by surgery is relatively cumbersome, time-consuming, laborious and traumatic.

[0004] To achieve the above-mentioned purpose, the technical scheme provided by the present application is as follows:

[0005] A PDX model tumor specimen implantation device, comprising an implantation cylinder, a piston head, a piston rod, a plug, a sharp head, a connecting frame, a support frame, a universal ball, a first locking component, a support column and a base; both ends of the implantation cylinder are open; the piston head is connected to one end of the piston rod; the sharp head is detachably connected to one end of the piston head away from the piston rod; the sharp head is conical, and the maximum diameter of the sharp head is consistent with the diameter of the piston head; the piston head is slidingly embedded in the implantation cylinder; one end of the piston rod away from the sharp head protrudes from the opening of the implantation cylinder; a material inlet communicating with the inside of the implantation cylinder is formed on the side of the implantation cylinder close to the sharp head; a material inlet cylinder covering the material inlet is connected to the outer wall of the implantation cylinder; the plug is detachably connected to one end of the piston head away from the piston rod; the implantation cylinder is detachably connected to the connecting frame; the connecting frame is slidingly connected to the support frame; the universal ball is connected to the support frame; the universal ball is movably connected to the top of the support column; the first locking component is arranged on the support column to lock the universal ball, thereby locking the angle of the implantation cylinder relative to the support column; the bottom of the support column is provided with the base.

[0006] Preferably, the support column top is formed with a first connecting block and a first inner cavity below the first connecting block; the support column is provided with a second connecting block below the first inner cavity; the support column is formed with a second inner cavity below the second connecting block; the first connecting block is provided with an arc-shaped through hole; and the universal ball is movably embedded in the arc-shaped through hole.

[0007] Preferably, the inner wall of the arc-shaped through hole is in contact with the outer wall of the universal ball; the first connecting block comprises a first outer wall and a second outer wall parallel to each other; the two ends of the arc-shaped through hole pass through the first outer wall and the second outer wall respectively; the first outer wall and the second outer wall are symmetrical about the center of the universal ball; the center of the universal ball is on the vertical central axis of the arc-shaped through hole; the top of the universal ball protrudes upward from the first outer wall; the bottom of the universal ball protrudes downward from the second outer wall, and the bottom of the universal ball is in the first inner cavity.

[0008] Preferably, the first locking component comprises a locking block, a first spring, a first sliding rod and a second sliding rod; the locking block and the first spring are arranged in the first inner cavity; the first sliding rod and the second sliding rod are slidably arranged in the second connecting block; the first sliding rod is parallel to the second sliding rod, and the first sliding rod and the second sliding rod are symmetrical about the center of the universal ball; one end of the first sliding rod is connected to the locking block, and the other end of the first sliding rod extends into the second inner cavity; one end of the second sliding rod is connected to the locking block, and the other end of the second sliding rod extends into the second inner cavity; one end of the first spring is connected to the side of the second connecting block facing the first connecting block, and the other end of the first spring is connected to the side of the locking block away from the universal ball; the first spring is always in a compressed state; the side of the locking block facing the universal ball is provided with a groove; the groove is in contact with the bottom of the universal ball to fix the universal ball by the elastic force of the first spring.

[0009] Preferably, the first locking component further comprises a connecting plate and an electromagnet; the connecting plate and the electromagnet are in the second inner cavity; the first sliding rod and the second sliding rod are connected to the connecting plate; the connecting plate is parallel to the first outer wall; the electromagnet is arranged on the side of the connecting plate away from the second connecting block; the electromagnet is used to attract the connecting plate; when the electromagnet attracts the connecting plate, the locking block and the universal ball are separated; the first spring is between the first sliding rod and the second sliding rod.

[0010] Preferably, the support column further comprises a control switch and a battery; the control switch is arranged on the outer wall of the support column; the battery is arranged in the second inner cavity; the battery is used for powering the electromagnet; the control switch is used for controlling the start and stop of the electromagnet.

[0011] Preferably, the support column further comprises a second locking component; the connecting frame comprises a first connecting arm, a second connecting arm, a third sliding rod and a fourth sliding rod; the first connecting arm is parallel to the second connecting arm; the third sliding rod is parallel to the fourth sliding rod; the two ends of the third sliding rod are connected to the first connecting arm and the second connecting arm respectively; the two ends of the fourth sliding rod are connected to the first connecting arm and the second connecting arm respectively; the middle part of the first connecting arm is provided with a first through hole, and the middle part of the second connecting arm is provided with a second through hole; the first through hole and the second through hole are opposite to each other; the first connecting arm is perpendicular to the third sliding rod; the implanting cylinder is used for sequentially penetrating through the first through hole and the second through hole; the second locking component is used for fixedly connecting the implanting cylinder to the connecting frame.

[0012] Preferably, the support frame comprises a connecting rod, a third connecting block, a fourth connecting block and a fifth connecting block; the fourth connecting block and the fifth connecting block are connected to the two ends of the third connecting block respectively; the fourth connecting block and the fifth connecting block are opposite to each other; one end of the connecting rod is connected to the side of the third connecting block away from the fourth connecting block; the other end of the connecting rod is connected to the universal ball; the third sliding rod is slidably arranged in the fourth connecting block; the fourth sliding rod is slidably arranged in the fifth connecting block.

[0013] Preferably, the support frame further comprises a reinforcing rod, a sleeve ring, a threaded rod and a screw cap; the third connecting block is further connected with a third connecting arm; the third connecting arm is parallel to the third sliding rod; the central axis of the implanting cylinder is parallel to the third sliding rod; the sleeve ring is slidably sleeved on the support column; one end of the reinforcing rod is universally connected to the end of the third connecting arm away from the third connecting block; the other end of the reinforcing rod is universally connected to the outer wall of the sleeve ring; the sleeve ring is provided with a threaded hole; the threaded rod is cooperatively screwed in the threaded hole; the end of the threaded rod away from the support column is provided with the screw cap; the threaded rod is used for abutting against the outer wall of the support column to fix the sleeve ring.

[0014] Preferably, the second locking component comprises a first connecting column, a second connecting column, a rotating shaft, a fifth sliding rod, a second spring, a first baffle and a second baffle; the outer wall of the implanting cylinder is sleeved with an annular baffle; the first connecting column and the second connecting column are both arranged at the top of the first connecting arm; the first connecting column is parallel to the second connecting column; the two ends of the rotating shaft are rotationally connected to the first connecting column and the second connecting column respectively; the rotating shaft is perpendicular to the third sliding rod, and the rotating shaft is perpendicular to the supporting column; the fifth sliding rod is slidingly arranged in the rotating shaft, and the fifth sliding rod is perpendicular to the rotating shaft; the first baffle and the second baffle are vertically connected to the two ends of the fifth sliding rod respectively, and the first baffle and the second baffle are respectively located on the two sides of the rotating shaft; the second spring is sleeved on the fifth sliding rod; one end of the second spring is connected to the rotating shaft, and the other end of the second spring is connected to the second baffle; the second spring is always in a compressed state; the elastic force of the second spring makes the first baffle have a tendency to abut against the rotating shaft; the first baffle is used for abutting against the annular baffle, so that the annular baffle abuts against the first connecting arm, so as to fixedly connect the implanting cylinder to the connecting frame.

[0015] Compared with the prior art, the present application has at least the following beneficial effects:

[0016] The PDX model tumor specimen implanting device can implant the specimen into the mouse body more safely, conveniently and quickly; when the device is operated, the operator first fixes the mouse on the operation table, then places the device on the operation table and close to the mouse; rotates the implanting cylinder to align the implanting cylinder with the position to be implanted in the mouse, then locks the position and angle of the implanting cylinder through the first locking component, then synchronously pushes the implanting cylinder and the piston rod to embed the sharp head into the position to be implanted in the mouse (since the connecting frame is slidingly connected to the supporting frame, the implanting cylinder can be moved forward or backward while keeping the angle of the implanting cylinder relative to the supporting column), and the position to be implanted in the mouse can be the abdominal cavity or subcutaneous; during the pushing process, the sharp head will pierce the mouse skin to form a wound and synchronously drive the implanting cylinder to extend into the mouse body; then stop moving the implanting cylinder and pull out the piston rod backward, then unscrew the sharp head on the piston head and screw the plug onto the piston head, then re-embed the piston head into the implanting cylinder and make the piston head behind the feeding port, then put the specimen into the implanting cylinder through the feeding port, and then manually push the piston rod to push the specimen forward, so that the specimen is pushed out from the end of the implanting cylinder in the mouse body to implant into the mouse body; after the implantation is completed, the implanting cylinder and the piston rod are pulled in the reverse direction to pull the implanting cylinder out of the mouse body; the whole operation process is safer, more convenient and more efficient than the traditional PDX model tumor specimen implanting operation. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or prior art description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by persons of ordinary skill in the art without creative effort based on these drawings.

[0018] Figure 1 Structure diagram of the implantation device for PDX model tumor specimen according to an embodiment of the present application;

[0019] Figure 2 Structure diagram of the implantation device for PDX model tumor specimen according to an embodiment of the present application; Figure 1 Structure diagram of the implantation device for PDX model tumor specimen according to an embodiment of the present application;

[0020] Figure 3 Structure diagram of the implantation device for PDX model tumor specimen according to an embodiment of the present application; Figure 1 Structure diagram of the implantation device for PDX model tumor specimen according to an embodiment of the present application;

[0021] Figure 4 Structure diagram of the implantation device for PDX model tumor specimen according to an embodiment of the present application;

[0022] Figure 5 Structure diagram of the implantation device for PDX model tumor specimen according to an embodiment of the present application;

[0023] Figure 6 Structure diagram of the implantation device for PDX model tumor specimen according to an embodiment of the present application;

[0024] Figure 7 Structure diagram of the implantation device for PDX model tumor specimen according to an embodiment of the present application.

[0025] Legend of reference signs:

[0026] 110, implant barrel; 120, piston rod; 130, end cap; 140, pointed head; 150, first connecting arm; 160, second connecting arm; 170, third sliding rod; 180, fourth sliding rod; 190, fourth connecting block; 210, fifth connecting block; 220, third connecting block; 230, third connecting arm; 240, connecting rod; 250, universal ball; 260, support column; 270, base; 280, reinforcing rod; 290, collar; 310, threaded rod; 320, screw cap; 330, feed barrel; 340, annular baffle; 350, first connecting column; 360, rotating shaft; 370, fifth sliding rod; 380, second spring; 390, first baffle; 410, second baffle; 420, plug; 430, piston head; 440, rubber sleeve; 450, feed port; 460, arc-shaped through hole; 470, first connecting block; 480, first outer wall; 490, second outer wall; 510, first inner cavity; 520, second inner cavity; 530, second connecting block; 540, locking block; 550, groove; 560, first spring; 570, first sliding rod; 580, second sliding rod; 590, connecting plate; 610, electromagnet.

[0027] The implementation, functional features and advantages of the present application will be further described with reference to the embodiments and the accompanying drawings. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0029] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present application are only used to explain the relative positional relationship, movement condition, etc. between components in a certain posture (as shown in the drawings), and if the certain posture changes, the directional indications also change accordingly.

[0030] In addition, the description such as "first", "second" and the like in the present application is only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "multiple" is at least two, such as two, three, etc., unless otherwise specifically limited.

[0031] In the present application, unless otherwise explicitly specified and limited, the terms "connection", "fixation" and the like shall be understood in a broad sense, for example, "fixation" can be fixed connection, or detachable connection, or integral; can be mechanical connection, or electrical connection; can be direct connection, or indirect connection through intermediate medium, can be internal communication of two elements or interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0032] In addition, the technical solutions among various embodiments of the present application can be combined with each other, but it must be based on the fact that a person skilled in the art can realize it, when the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist, and is not within the protection scope required by the present application.

[0033] The present application provides a PDX model tumor specimen implantation device.

[0034] As shown in the accompanying Figure 1 -attached Figure 7 In an embodiment of the PDX model tumor specimen implantation device provided by the present application, the PDX model tumor specimen implantation device comprises an implantation cylinder 110, a piston head 430, a piston rod 120, a plug 420, a sharp head 140, a connecting frame, a supporting frame, a universal ball 250, a first locking component, a supporting column 260 and a base 270; both ends of the implantation cylinder 110 are open; the piston head 430 is connected to one end of the piston rod 120; the sharp head 140 is detachably connected to the end of the piston head 430 away from the piston rod 120; the sharp head 140 is conical, and the maximum diameter of the sharp head 140 is consistent with the diameter of the piston head 430 (since the size of the tumor specimen is generally 5-8 mm, and the size of the mouse incision is generally 1 cm, the maximum diameter of the sharp head 140 in this embodiment is 5-8 mm, and the maximum diameter of the sharp head 140 is consistent with the inner diameter of the implantation cylinder 110); the piston head 430 is slidingly embedded in the implantation cylinder 110; the end of the piston rod 120 away from the sharp head 140 protrudes out of the opening of the implantation cylinder 110; a feeding port 450 communicating with the inside of the implantation cylinder 110 is formed on the side of the implantation cylinder 110 close to the sharp head 140; a feeding cylinder 330 covering the feeding port 450 is connected to the outer wall of the implantation cylinder 110; the plug 420 is detachably connected to the end of the piston head 430 away from the piston rod 120; the implantation cylinder 110 is detachably connected to the connecting frame; the connecting frame is slidingly connected to the supporting frame; the universal ball 250 is connected to the supporting frame; the universal ball 250 is movably connected to the top of the supporting column 260; the first locking component is arranged on the supporting column 260 to lock the universal ball 250, thereby locking the angle of the implantation cylinder 110 relative to the supporting column 260; the bottom of the supporting column 260 is provided with the base 270.

[0035] Specifically, as shown in Figs. Figure 1 , 4 , 6 and 7, the end of the piston head 430 away from the piston rod 120 is provided with a threaded hole (not shown), the flat end of the sharp head 140 is provided with a first threaded rod (not labeled) capable of being screwed into the threaded hole, one side of the plug 420 is provided with a second threaded rod (not labeled) capable of being screwed into the threaded hole; the end of the piston rod 120 protruding from the implanting cylinder 110 is detachably provided with an end cap 130; the piston rod 120 is further sleeved with a rubber sleeve 440; the rubber sleeve 440 is in sliding abutment with the inner wall of the implanting cylinder 110; when the sharp head 140 just protrudes from one end of the implanting cylinder (as shown in Fig. Figure 4 ), the rubber sleeve 440 is located at the side of the material inlet 450 close to the end cap 130 (i.e. the material inlet 450 is between the piston head 430 and the rubber sleeve 440); the piston head 430 and the rubber sleeve 440 are both made of rubber.

[0036] The PDX model tumor specimen implanting device provided by the application can more safely, conveniently and quickly implant the specimen into the mouse body; when operating the device, the operator first fixes the mouse on the operation table, then places the device on the operation table and close to the mouse; rotates the implanting cylinder 110 to align the implanting cylinder 110 with the position to be implanted in the mouse, then locks the position and angle of the implanting cylinder 110 through the first locking member, and then synchronously pushes the implanting cylinder 110 and the piston rod 120 to embed the sharp head 140 into the position to be implanted in the mouse (since the connecting frame is slidingly connected to the support frame, the implanting cylinder 110 can be moved forward or backward while keeping the angle of the implanting cylinder 110 relative to the support column 260), the position to be implanted in the mouse can be the abdominal cavity or subcutaneous, during the pushing process, the sharp head 140 will pierce the mouse skin to form a wound, and simultaneously drive the implanting cylinder 110 to extend into the mouse body; then stop moving the implanting cylinder 110 and pull out the piston rod 120 backward, then unscrew the sharp head 140 on the piston head 430, and screw the plug 420 onto the piston head 430, then re-embed the piston head 430 into the implanting cylinder 110, and make the piston head 430 behind the material inlet 450, then put the specimen into the implanting cylinder 110 through the material inlet 450, and then manually push the piston rod 120 to push the specimen forward, and then push the specimen out of the end of the implanting cylinder 110 in the mouse body to implant into the mouse body; after the implantation is completed, the implanting cylinder 110 is pulled out of the mouse body in the reverse direction; the whole operation process is more safe, convenient and efficient compared with the traditional PDX model tumor specimen implanting operation, and the wound is smaller compared with the traditional surgical incision since the sharp head is used to pierce the mouse.

[0037] In addition, as shown in Figs. Figure 2As shown, the top of the support column 260 is formed with a first connecting block 470 and a first inner cavity 510 below the first connecting block 470; the inside of the support column 260 is provided with a second connecting block 530 below the first inner cavity 510; the support column 260 is formed with a second inner cavity 520 below the second connecting block 530; the first connecting block 470 is provided with an arc-shaped through hole 460; and the universal ball 250 is movably embedded in the arc-shaped through hole 460.

[0038] Meanwhile, the inner wall of the arc-shaped through hole 460 is in contact with the outer wall of the universal ball 250; the first connecting block 470 includes a first outer wall 480 and a second outer wall 490 which are parallel to each other; the two ends of the arc-shaped through hole 460 pass through the first outer wall 480 and the second outer wall 490 respectively; the first outer wall 480 and the second outer wall 490 are symmetrical about the center of the universal ball 250; the center of the universal ball 250 is on the vertical central axis of the arc-shaped through hole 460; the top of the universal ball 250 protrudes upward from the first outer wall 480; the bottom of the universal ball 250 protrudes downward from the second outer wall 490, and the bottom of the universal ball 250 is in the first inner cavity 510. Through the above technical scheme, the specific structure of the universal ball 250 connected to the support column 260 is improved.

[0039] In addition, the first locking member includes a locking block 540, a first spring 560, a first sliding rod 570 and a second sliding rod 580; the locking block 540 and the first spring 560 are arranged in the first inner cavity 510; the first sliding rod 570 and the second sliding rod 580 are slidably arranged in the second connecting block 530; the first sliding rod 570 is parallel to the second sliding rod 580, and the first sliding rod 570 and the second sliding rod 580 are symmetrical about the center of the universal ball 250; one end of the first sliding rod 570 is connected to the locking block 540, and the other end of the first sliding rod 570 extends into the second inner cavity 520; one end of the second sliding rod 580 is connected to the locking block 540, and the other end of the second sliding rod 580 extends into the second inner cavity 520; one end of the first spring 560 is connected to the side of the second connecting block 530 facing the first connecting block 470, and the other end of the first spring 560 is connected to the side of the locking block 540 away from the universal ball 250; the first spring 560 is always in a compressed state; the side of the locking block 540 facing the universal ball 250 is provided with a groove 550 (a rubber anti-slip layer is arranged in the groove 550); the elastic force of the first spring 560 makes the groove 550 abut against the bottom of the universal ball 250 to fix the universal ball 250.

[0040] Meanwhile, the first locking component also includes a connecting plate 590 and an electromagnet 610; both the connecting plate 590 and the electromagnet 610 are located in the second inner cavity 520; the first slide rod 570 and the second slide rod 580 are both connected to the connecting plate 590; the connecting plate 590 is parallel to the first outer wall 480; the electromagnet 610 is located on the side of the connecting plate 590 opposite to the second connecting block 530; the electromagnet 610 is used to attract the connecting plate 590; when the electromagnet 610 attracts the connecting plate 590, the locking block 540 and the universal ball 250 separate; the first spring 560 is located between the first slide rod 570 and the second slide rod 580. This PDX model tumor specimen implantation device also includes a control switch and a battery; the control switch is located on the outer wall of the support column 260; the battery is located in the second inner cavity 520; the battery is used to power the electromagnet 610; the control switch is used to control the start and stop of the electromagnet 610.

[0041] The above technical solution further improves the structure and function of the first locking component. When it is necessary to adjust the angle of the implantation cylinder 110 relative to the support column 260 to align with the implantation position of the mouse, the electromagnet 610 is activated by the control switch, thereby attracting the connecting plate 590 to separate the locking block 540 and the universal ball 250. At this time, the angle of the implantation cylinder 110 relative to the support column 260 can be rotated normally. After the adjustment is completed, the electromagnet 610 is stopped by the control switch. Under the action of the first spring 560, the locking block 540 abuts against the bottom of the universal ball 250 (specifically, the groove 550 fits against the bottom of the universal ball 250) to fix the universal ball 250, thereby fixing the angle of the implantation cylinder 110 relative to the support column 260.

[0042] In addition, as attached Figure 1 and attached Figure 5 As shown, the PDX model tumor specimen implantation device also includes a second locking component; the connecting frame includes a first connecting arm 150, a second connecting arm 160, a third sliding rod 170, and a fourth sliding rod 180; the first connecting arm 150 is parallel to the second connecting arm 160; the third sliding rod 170 is parallel to the fourth sliding rod 180; the two ends of the third sliding rod 170 are respectively connected to the first connecting arm 150 and the second connecting arm 160; the two ends of the fourth sliding rod 180 are respectively connected to the first connecting arm 150 and the second connecting arm 160; a first through hole is opened in the middle of the first connecting arm 150, and a second through hole is opened in the middle of the second connecting arm 160; the first through hole and the second through hole are directly opposite each other; the first connecting arm 150 is perpendicular to the third sliding rod 170; the implantation cylinder 110 is used to pass through the first through hole and the second through hole in sequence; the second locking component is used to fix the implantation cylinder 110 to the connecting frame.

[0043] Meanwhile, the support frame includes a connecting rod 240, a third connecting block 220, a fourth connecting block 190, and a fifth connecting block 210; the fourth connecting block 190 and the fifth connecting block 210 are respectively connected to the two ends of the third connecting block 220; the fourth connecting block 190 and the fifth connecting block 210 are facing each other; one end of the connecting rod 240 is connected to the side of the third connecting block 220 away from the fourth connecting block 190; the other end of the connecting rod 240 is connected to the universal ball 250; the third sliding rod 170 slides through the fourth connecting block 190; the fourth sliding rod 180 slides through the fifth connecting block 210.

[0044] The above technical solution improves the specific structure of the support frame and the connecting frame. Because the third slide rod 170 slides through the fourth connecting block 190 and the fourth slide rod 180 slides through the fifth connecting block 210, the connecting frame can slide relative to the support frame as a whole, thus driving the implantation tube 110 to move axially so that the tip 140 can be embedded in the mouse's body for specimen implantation.

[0045] In addition, as attached Figure 1 As shown, the PDX model tumor specimen implantation device also includes a reinforcing rod 280, a collar 290, a threaded rod 310, and a cap 320; the third connecting block 220 is also connected to a third connecting arm 230; the third connecting arm 230 is parallel to the third sliding rod 170; the central axis of the implantation cylinder 110 is parallel to the third sliding rod 170; the collar 290 is slidably sleeved on the support column 260; one end of the reinforcing rod 280 is universally hinged to the end of the third connecting arm 230 away from the third connecting block 220; the other end of the reinforcing rod 280 is universally hinged to the outer wall of the collar 290; the collar 290 has a through threaded hole; the threaded rod 310 is screwed into the threaded hole; the end of the threaded rod 310 away from the support column 260 is provided with a cap 320; the threaded rod 310 is used to abut against the outer wall of the support column 260 to fix the collar 290.

[0046] With the above technical solution, when adjusting the angle of the implantation tube 110 relative to the support column 260, the threaded rod 310 is loosened, allowing the collar 290 to slide freely vertically relative to the support column 260. Since the reinforcing rod 280, the collar 290, and the third connecting arm 230 are all universally hinged, the reinforcing rod 280 will not restrict the rotation of the implantation tube 110. After the angle of the implantation tube 110 is adjusted, the threaded rod 310 is tightened, so that the threaded rod 310 abuts against the outer wall of the support column 260 to fix the position of the collar 290. Thus, the reinforcing rod 280 provides a more stable support effect for the support frame, connecting frame, and implantation tube 110.

[0047] Meanwhile, as attached Figure 3As shown, the second locking component includes a first connecting column 350, a second connecting column, a rotating shaft 360, a fifth sliding rod 370, a second spring 380, a first baffle 390 and a second baffle 410; the outer wall of the implanting cylinder 110 is sleeved with an annular baffle 340; the first connecting column 350 and the second connecting column are both arranged at the top of the first connecting arm 150; the first connecting column 350 is parallel to the second connecting column; the two ends of the rotating shaft 360 are rotatably connected to the first connecting column 350 and the second connecting column respectively; the rotating shaft 360 is perpendicular to the third sliding rod 170, and the rotating shaft 360 is perpendicular to the supporting column 260; the fifth sliding rod 370 is slidably arranged in the rotating shaft 360, and the fifth sliding rod 370 is perpendicular to the rotating shaft 360; the first baffle 390 and the second baffle 410 are vertically connected to the two ends of the fifth sliding rod 370 respectively, and the first baffle 390 and the second baffle 410 are respectively located on the two sides of the rotating shaft 360; the second spring 380 is sleeved on the fifth sliding rod 370; one end of the second spring 380 is connected to the rotating shaft 360, and the other end of the second spring 380 is connected to the second baffle 410; the second spring 380 is always in a compressed state; the elastic force of the second spring 380 makes the first baffle 390 have a tendency to abut against the rotating shaft 360; the first baffle 390 is used for abutting against the annular baffle 340, so that the annular baffle 340 abuts against the first connecting arm 150, so as to fixedly connect the implanting cylinder 110 to the connecting frame.

[0048] Through the above technical scheme, the specific structure of the second locking component is improved. When it is needed to lock and fix the implanting cylinder 110 to the connecting frame, the implanting cylinder 110 is sequentially arranged through the first through hole and the second through hole, and the annular baffle 340 is arranged close to and abuts against the first connecting arm 150, then the second baffle 410 is pushed and the fifth sliding rod 370 is rotated, so that the first baffle 390 is arranged on the side of the annular baffle 340 away from the first connecting arm 150, then the second baffle 410 is released, and under the elastic force of the second spring 380, the first baffle 390 abuts against the annular baffle 340, so that the annular baffle 340 is tightly pressed against the first connecting arm 150, thereby fixing the implanting cylinder 110 to the connecting frame; when it is needed to release and disassemble the implanting cylinder 110 (for example, cleaning the implanting cylinder 110), the second baffle 410 is pushed again and the fifth sliding rod 370 is rotated, so that the first baffle 390 no longer abuts against the annular baffle 340 and is arranged above the annular baffle 340, at this time, the implanting cylinder 110 can be pulled out from the connecting frame.

[0049] The above only describes the preferred embodiments of the present application, and does not limit the patent scope of the present application, and any equivalent structural transformation made under the inventive concept of the present application, or direct / indirect application in other related technical fields is included in the patent protection scope of the present application.

Claims

1. A PDX model tumor specimen implantation device, characterized by, The utility model provides implanting cylinder, piston head, piston rod, plug, sharp head, connecting frame, support frame, universal ball, first locking part, support column and base are included, the piston head is connected with one end of piston rod, the sharp head and plug are respectively detachable connection with the one end of piston head away from piston rod, the sharp head is conical, and the maximum diameter of sharp head and the diameter of piston head are consistent, the one end of piston rod away from sharp head is protruding in the open mouth of implanting cylinder, the side of implanting cylinder close to sharp head is equipped with the feed inlet that communicates implanting cylinder inside, the outer wall of implanting cylinder is connected with the feed cylinder of cover feed inlet, implanting cylinder is detachable connection with connecting frame, connecting frame is connected with support frame in sliding, universal ball is connected with support frame and is connected with the top of support column, the bottom of support column is provided with base, the top of support column forms first connecting block and first inner chamber under first connecting block, the inside of support column is provided with second connecting block under first inner chamber, the second inner chamber is formed in support column under second connecting block, the first connecting block is equipped with arc through -hole, the universal ball is movably embedded in arc through -hole, The first locking part includes locking block, first spring, first sliding rod and second sliding rod, the locking block and first spring are arranged in first inner chamber, the first sliding rod and second sliding rod are slidably arranged in second connecting block, one end of first sliding rod and second sliding rod is connected with locking block, the other end is inserted into second inner chamber, one end of first spring is connected with the side of second connecting block towards first connecting block, the other end of first spring is connected with the side of locking block away from universal ball, The first locking part further includes connecting plate and electromagnet, the connecting plate and electromagnet are in second inner chamber, the first sliding rod and second sliding rod are connected with connecting plate, the electromagnet is used for attracting connecting plate, The connecting frame includes first connecting arm, second connecting arm, third sliding rod and fourth sliding rod, the first connecting arm is parallel to second connecting arm, the third sliding rod is parallel to fourth sliding rod, the middle part of first connecting arm and second connecting arm is respectively equipped with first through -hole and second through -hole, the first connecting arm is perpendicular to third sliding rod, implanting cylinder is used for passing through first through -hole and second through -hole in sequence, The second locking component comprises a first connecting column, a second connecting column, a rotating shaft, a fifth sliding rod, a second spring, a first baffle and a second baffle; the outer wall of the implanting cylinder is sleeved with an annular baffle; the first connecting column and the second connecting column are arranged in parallel on the top of the first connecting arm; the two ends of the rotating shaft are rotationally connected to the first connecting column and the second connecting column respectively; the fifth sliding rod is vertically and slidably arranged in the rotating shaft; the first baffle and the second baffle are vertically connected to the two ends of the fifth sliding rod respectively, and the first baffle and the second baffle are located on the two sides of the rotating shaft respectively; the second spring is sleeved on the fifth sliding rod; one end of the second spring is connected to the rotating shaft, and the other end of the second spring is connected to the second baffle; the first baffle is used for abutting against the annular baffle, so that the annular baffle abuts against the first connecting arm.

2. The PDX model tumor specimen implantation device of claim 1, wherein, The inner wall of the arc-shaped through hole is in contact with the outer wall of the universal ball; the first connecting block comprises a first outer wall and a second outer wall which are parallel to each other; the two ends of the arc-shaped through hole respectively penetrate the first outer wall and the second outer wall; the first outer wall and the second outer wall are symmetrical about the center of the universal ball; the center of the universal ball is located on the vertical central axis of the arc-shaped through hole; the top of the universal ball protrudes upward from the first outer wall; the bottom of the universal ball protrudes downward from the second outer wall, and the bottom of the universal ball is located in the first inner cavity.

3. The PDX model tumor specimen implantation device of claim 2, wherein, The first spring is always in a compressed state; the side of the locking block facing the universal ball is provided with a groove; the elastic force of the first spring makes the groove abut against the bottom of the universal ball to fix the universal ball.

4. The PDX model tumor specimen implantation device of claim 3, wherein, The connecting plate is parallel to the first outer wall; the electromagnet is arranged on the side of the connecting plate away from the second connecting block; when the electromagnet attracts the connecting plate, the locking block and the universal ball are separated; the first spring is between the first sliding rod and the second sliding rod.

5. The PDX model tumor specimen implantation device of claim 4, wherein, Further comprising a control switch and a storage battery; the control switch is arranged on the outer wall of the supporting column; the storage battery is arranged in the second inner cavity; the storage battery is used for supplying power to the electromagnet; the control switch is used for controlling the start and stop of the electromagnet.

6. The PDX model tumor specimen implantation device of claim 1, wherein, The two ends of the third sliding rod are respectively connected to the first connecting arm and the second connecting arm; the two ends of the fourth sliding rod are respectively connected to the first connecting arm and the second connecting arm.

7. The PDX model tumor specimen implantation device of claim 6, wherein, The supporting frame comprises a connecting rod, a third connecting block, a fourth connecting block and a fifth connecting block; the fourth connecting block and the fifth connecting block are respectively connected to the two ends of the third connecting block; the fourth connecting block and the fifth connecting block are opposite to each other; one end of the connecting rod is connected to the side of the third connecting block away from the fourth connecting block; the other end of the connecting rod is connected to the universal ball; the third sliding rod is slidably arranged in the fourth connecting block; the fourth sliding rod is slidably arranged in the fifth connecting block.

8. The PDX model tumor specimen implantation device of claim 7, wherein, The third connecting block is further connected with a third connecting arm; the third connecting arm is parallel to the third sliding rod; the middle axis of the implanting cylinder is parallel to the third sliding rod; the sleeve ring is slidingly sleeved on the supporting column; one end of the reinforcing rod is universally connected to the end of the third connecting arm away from the third connecting block; the other end of the reinforcing rod is universally connected to the outer wall of the sleeve ring; the sleeve ring is provided with a threaded hole penetrating therethrough; the threaded rod is cooperatively screwed in the threaded hole; the threaded rod is provided with the screw cap at the end away from the supporting column; the threaded rod is used for abutting against the outer wall of the supporting column to fix the sleeve ring.

9. The PDX model tumor specimen implantation device of claim 6, wherein, The second spring is always in a compressed state; the elastic force of the second spring makes the first baffle have a tendency to abut against the rotating shaft.

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