Vitamin B12-containing scalp care composition with hair growth promoting effect

Abstract

The invention relates to a vitamin B12-containing scalp care composition with a hair growth promoting effect. The vitamin B12-containing scalp care composition is characterized by containing 0.0001-0.0003% by mass of vitamin B12. The vitamin B12 essence further consists of the following components in percentage by weight: 0.0001 to 0.0003 percent of vitamin B12, 5.0 to 20.0 percent of compound active matter, 0.01 to 0.5 percent of ginger essential oil, 0.02 to 0.50 percent of lavender essential oil, 0.02 to 0.50 percent of rosemary essential oil, 5.0 to 20.0 percent of polyhydric alcohol, 0.02 to 2.0 percent of pH regulator, 0.1 to 2.0 percent of solubilizer and the balance of pure water. The composition disclosed by the invention has an effect of promoting hair growth, and particularly has a repairing effect on DNA damage of hair papilla cells and an anti-oxidation (ROS) effect of the hair papilla cells.

Description

Technical Field

[0001] The present invention belongs to the field of daily chemical products, and in particular, relates to a product containing vitamin B 12 A scalp care composition having a hair growth promoting effect. Background Art

[0002] Due to factors such as genetics, environment, excessive mental stress, staying up late, and drugs (such as chemotherapy-induced hair loss), the proportion of modern people losing hair is increasing, and hair loss is becoming more and more common among young people. According to the 2017 "China Hair Loss Population Survey Report", there are currently about 200 million people with hair loss in my country, and the rate is increasing at a rate of at least 15%. Most of them are men, with a prevalence rate of about 21.3%, and the prevalence rate of women is about 6.0%. For male hair loss, common types include androgenic alopecia and alopecia areata, of which more than 90% is androgenic alopecia.

[0003] Faced with problems such as hair loss, hair loss and hair thinning, most people tend to focus directly on strategies to prevent hair loss, but often ignore the importance of hair follicles. Hair follicles are the source of hair growth, and the integrity of their structure is not only required for the body's metabolism, but also has important significance for a person's appearance. Hair follicles have the characteristics of self-renewal and cyclical growth, which is closely related to a group of mesenchymal cells in the hair bulb, namely, hair papilla cells, which play an important inductive role in hair follicle development and the postnatal hair follicle cycle. Without hair papilla cells, hair follicles cannot be formed; in the postnatal hair follicle cycle, hair papilla cells directly or indirectly induce the cyclical growth of hair follicles by interacting with the surrounding hair follicle epithelial cells. Therefore, maintaining or restoring the hair follicle induction function of hair papilla cells has become a focus of research on hair loss treatment.

[0004] At present, the quality of anti-hair loss products containing natural plant or traditional Chinese medicine extracts on the market varies greatly, or contain high concentrations of ethanol, which can easily irritate the scalp and aggravate hair loss. Therefore, it is of great practical significance to develop an effective composition that can maintain hair follicles, nourish hair roots, protect hair, and prevent hair loss. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a vitamin B 12 The scalp care composition has the function of promoting hair growth, and the composition has the function of repairing DNA damage of hair papilla cells and anti-oxidation (ROS) of hair papilla cells.

[0006] In order to solve the above technical problems, the present invention adopts the following technical scheme: a 12 A scalp care composition having a hair growth promoting effect, characterized in that it contains 0.0001 to 0.0003% by mass of vitamin B12 .

[0007] Further, as a further improvement of the present invention, a vitamin B 12 The scalp care composition having the effect of promoting hair growth is characterized in that it is composed of the following components in percentage by mass: 0.0001-0.0003% of vitamin B 12 , 5.0~20.0% compound active ingredients, 0.01~0.5% ginger essential oil, 0.02~0.50% lavender essential oil, 0.02~0.50% rosemary essential oil, 5.0~20.0% polyol, 0.02~2.0% pH adjuster, 0.1~2.0% solubilizer, and the balance is pure water; the compound active ingredient is one of panthenol, niacinamide, pyridoxine hydrochloride, biotin, or a mixture thereof in any mass ratio.

[0008] Furthermore, the mass ratio of the rosemary essential oil, the lavender essential oil and the ginger essential oil is 3:12:10.

[0009] The polyol includes one of glycerol, 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, propylene glycol, butylene glycol, caprylyl glycol, ethylhexylglycerol, or a mixture thereof in any mass ratio.

[0010] The pH regulator is one of citric acid, sodium citrate, arginine, tromethamine, aminomethyl propanol, or a mixture thereof in any mass ratio.

[0011] The solubilizer is one of PEG-40 hydrogenated castor oil, cocoether-7, PPG-1-PEG-9 lauryl glycol ether, or a mixture thereof in any mass ratio.

[0012] The present invention also provides a kind of vitamin B 12 The scalp care composition is prepared by the following method, comprising the following steps: First, accurately weigh each raw material according to the mass percentage; then, at room temperature, mix the weighed pure water, compound active ingredients, and polyols, and stir until dissolved; then add a pH adjuster, stir and dissolve to obtain phase A; evenly mix the weighed solubilizer with lavender essential oil, rosemary essential oil, and ginger essential oil, then add the polyol and stir evenly to obtain phase B; pour phase A into phase B while stirring, and mix evenly to obtain a vitamin B-containing 12 Scalp care composition.

[0013] The present invention provides a kind of vitamin B containing 0.0001-0.0003% 12 Mass fraction of scalp care composition, vitamin B in the composition 12 (Vitamin B 12), also known as cobalamin, is a general term for cobalt-containing corrin compounds. Vitamin B 12 The appearance is a red crystalline solid with no special smell. Its aqueous solution is neutral and its properties are stable. The applicant has confirmed through in vitro experiments that vitamin B 12 It can inhibit the generation of reactive oxygen species (ROS) in the oxidation of human hair papilla cells, and has a protective and repairing effect on human hair papilla cells affected by oxidative damage. It can prevent human hair papilla cells from apoptosis and promote hair growth.

[0014] Niacinamide, also known as vitamin B 3 , which can promote cell metabolism. Niacinamide will be converted into niacin in the body, penetrate into the lower blood vessels through the hair follicles, promote vascular dilation and blood circulation, and apply it to shampoo and hair care products to promote hair follicle microcirculation, promote hair growth, and improve the effect of hair loss.

[0015] Panthenol is a B vitamin 5 Panthenol is a precursor of hydroxybenzoic acid, which penetrates into the hair and is replaced by sebum, so the hair does not become heavy and the hairstyle can last longer. In hair care products, panthenol has the following advantages: long-term moisturizing of hair; improving hair combing; reducing the formation of split ends; improving hair damage; thickening hair and brightening the color.

[0016] Pyridoxine hydrochloride is vitamin B 6 , which can be quickly converted into coenzymes in the body - pyridoxal phosphate and pyridoxamine. It plays an important role in protein metabolism, and can resist sebum and promote hair regeneration. 6 It can reduce the hormone prolactin, thereby alleviating the atrophy of hair follicles, normalizing the protein and amino acid metabolism of the head skin, effectively adjusting the vitality of the sebaceous glands of the head skin, treating seborrheic dermatitis, and improving the condition of oily hair.

[0017] Biotin is vitamin B 7 , is a coenzyme of carboxylase and plays an important role in the metabolism of glucose, amino acids and fatty acids in mammals. It plays an important role in the metabolism of all macronutrients through four biotin-dependent carboxylases involved in fatty acid synthesis, gluconeogenesis and amino acid metabolism. Biotin deficiency may cause zinc or EFA deficiency, including hair loss, rash and central nervous system dysfunction.

[0018] Plant essential oils can promote scalp cell regeneration and balance scalp oil secretion. The preferred lavender essential oil, rosemary essential oil, ginger essential oil, etc. of the present invention are all natural plant essential oils commonly used in hair care products. The main component of plant "essential oils" for hair is fatty acid glyceride, which has a polar head. Therefore, plant essential oils have a stronger adsorption capacity for polar keratin in hair fibers and a better penetration effect. Some can even penetrate into the CMC layer of the hair cuticle. They protect the hair cuticle from swelling and falling off by strengthening the hydrophobic effect of the lipoprotein layer, thereby maintaining the integrity of the hair shaft.

[0019] Polyols are commonly used moisturizers in cosmetics. The hydrophilic groups in them can capture water molecules and have both moisturizing and antibacterial functions. According to research reports, polyols exert their antibacterial effects by interfering with the physiological environment of the cell membrane, destroying the cell membrane of microorganisms, and then forming vesicles or rupturing them. Polyols such as 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, propylene glycol, butanediol, caprylyl glycol, and ethylhexylglycerin with alcoholic hydroxyl groups are also good preservative promoters that can promote the preservative effects of a variety of traditional preservatives.

[0020] In summary, vitamin B 12 In the case of having a hair growth effect, the inventors compounded other ingredients with hair growth activity, and the appropriately compounded composition has better hair growth function, better anti-hair loss effect, and more comprehensive functional effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Contains vitamin B 12 Contains vitamin B 12 A product photo illustration of a scalp care composition; Figure 2 Vitamin B 12 For H 2 O 2 Comet assay fluorescence image of DNA damage repair induced by human dermal papilla cells; Figure 3 Vitamin B 12 Schematic diagram of the statistical results of the antioxidant (ROS) level of hair papilla cells; Figure 4 Schematic diagram of fluorescence contrast of dermal papilla cells in vitro. DETAILED DESCRIPTION

[0022] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0023] Test 1: Contains Vitamin B 12 Test on the repair efficacy of 0.0001~0.0003% mass concentration aqueous solution on DNA damage in dermal papilla cells The culture medium was 10% fetal bovine serum at 37.0 ± 1 °C and 5 ± 1% CO. 2 Dermal papilla cells were cultured under (v / v) and saturated humidity conditions.

[0024] MTT cytotoxicity assay: Vitamin B 12 Ultrapure water was used to prepare a series of concentrations of 0.000316%, 0.001%, 0.00316%, 0.01%, 0.0316%, and 0.1% for subsequent MTT experiments. The experimental steps were as follows: cells were inoculated in a 96-well plate, 0.1 mL / well (cell density was about 1.0-1.3×10 5 / mL), 37°C, 5% CO 2 Culture for 24 h. Discard the culture medium, add 0.1 mL of different culture medium to each well, add maintenance culture medium to the control group, and continue to culture for 24 h. Add 20 μL of MTT solution to each well and incubate for 3 h, then discard the liquid in each well, add 100 μL DMSO, shake in the dark for 15 min, and measure the absorbance (OD value) at 570 nm. The OD value of each well was corrected with the blank well. The test results are shown in Table 1: Vitamin B 12 Results of toxicity tests on dermal papilla cells.

[0025]

[0026] Table 1 Vitamin B 12 Results of toxicity test on dermal papilla cells

[0027] Note: Vitamin B 12 Purchased from DSM Nutritional Products Ltd., the concentration is 0.1% by mass.

[0028] H 2 O 2 Stimulate single cell gel electrophoresis test of dermal papilla cells: Inoculate cells in 6-well plates, 2 mL / well (cell density is about 1.0-1.3×10 5 / mL), 37°C, 5% CO 2 Culture for 24 h. Discard the culture medium, add 2 mL of culture medium containing samples of different concentrations to each well, add the test medium to the control group, and continue to culture for 2 h. 2 O 2After 1 h of treatment with 100 μM, the cells were harvested after replacing the maintenance medium with or without the test substance and continuing to culture for 1 h. The original culture medium was discarded, 200 μL of 0.25% trypsin-EDTA was added to the 6-well plate, and after digestion for 1 to 2 min, 400 μL of culture medium containing fetal bovine serum was added to terminate the digestion. The cell suspensions of each well were collected in 1.5 mL EP tubes, centrifuged at 1500 rpm / min for 10 min, and the supernatant was discarded. Wash with PBS twice, then add 100 μL PBS to resuspend the cells to make a cell suspension. After mixing the cell suspension with low melting point agar (1:10), 50 μL was added to a special glass slide and placed at 4°C for 10 min to solidify. The glass slide containing the cells was placed in the lysis solution and lysed at 4°C overnight. The glass slide was placed in the lysis solution and placed at room temperature away from light for 20 min. The slide was then placed in an electrophoresis tank containing about 850 mL of precooled alkaline electrophoresis solution and electrophoresed at 21 V for 30 min. The slide was removed and immersed in pure water for 5 min, repeated once, and then immersed in 70% ethanol for 5 min. After the slide was dried, the stain was added to the stain for 30 min in the dark, washed with pure water, and dried at 37°C. Observed under a fluorescence microscope (Ex494 nm, Em520 nm), and pictures were collected and analyzed using a dedicated comet analysis software. 100 cells were analyzed in each group.

[0029] Vitamin B 12 Three samples with mass concentrations of 0.0001%, 0.0002%, and 0.0003%, as well as negative controls and model controls were tested in parallel, and the analysis results were analyzed for significant differences. The statistical analysis results are shown in Table 2: Table 2 Results of DNA percentage in the tail of comet assay

[0030] # / ## / ###: Compared with the negative control, P<0.05 / 0.01 / 0.001, the difference is statistically significant; * / ** / ***: Compared with the model control, P<0.05 / 0.01 / 0.001, the difference is statistically significant.

[0031] As shown in Table 2, Vitamin B 12 At a mass concentration of 0.0001~0.0003%, H 2 O 2 The induced DNA damage in human dermal papilla cells has a good repair effect.

[0032] Test Example 2: Containing Vitamin B 12Test of the antioxidant (ROS) efficacy of 0.0001~0.0003% mass concentration aqueous solution on dermal papilla cells The cells were seeded in 6-well plates at 2 mL / well (cell density was approximately 1.0-1.3×10 5 / mL), 37°C, 5% CO 2 Culture for 24 h. Discard the culture medium, add 2 mL of culture medium containing samples of different concentrations (0.1%, 0.3%) to each well, and add maintenance culture medium to the control group, and continue to culture for 24 h. 2 O 2 (200 μM) for 1 h. After adding the fluorescent probe for 30 min, the cells were washed with PBS. Image acquisition / analysis: The cells were observed under a fluorescence microscope (Ex494 nm, Em520 nm), and images were collected. The fluorescence intensity of the images was analyzed using Image J. Three fields of view were randomly photographed in each group. The test results are shown in Table 3.

[0033] Table 3 Results of the anti-oxidation (ROS) test of samples on dermal papilla cells

[0034] # / ## / ###: Compared with the negative control, P<0.05 / 0.01 / 0.001, the difference is statistically significant; * / ** / ***: Compared with the model control, P<0.05 / 0.01 / 0.001, the difference is statistically significant.

[0035] As shown in Table 3, Vitamin B 12 The inhibition of ROS was concentration-dependent. 12 When the concentration was 0.0003%, the ROS inhibition rate reached 37.3%. 2 O 2 ) compared with the control group, the ROS content was significantly reduced, and it was believed that Vitamin B 12 At 0.0001% and 0.0003%, H 2 O 2 Induced oxidative damage in human dermal papilla cells has a protective effect. Example

[0036] Table 4 Scalp care composition 1# formula

[0037] A vitamin B 12 The scalp care composition, the mass ratio of each component is shown in Table 4, and is prepared by the following method: 1) Accurately weigh each material according to the mass percentage in Table 4 above; 2) Weigh pure water and vitamin B 12 , panthenol, niacinamide, 1,2-pentanediol, and 1,2-propylene glycol, and stir until dissolved; then add citric acid and sodium citrate, and stir to dissolve to obtain phase A; 3) Evenly mix the weighed PEG-40 hydrogenated castor oil with lavender essential oil, rosemary essential oil and ginger essential oil, then add butylene glycol and stir evenly to obtain phase B; 4) Pour phase A into phase B while stirring, and you will get a natural and pleasant fragrance, turmeric yellow, transparent and uniform, low viscosity, and vitamin B that has the function of hair growth. 12 Scalp care composition. Example

[0038] Table 5 Scalp care composition 2# formula

[0039] A vitamin B 12 The scalp care composition, the mass ratio of each component is shown in Table 5, and is prepared by the following method: 1) Accurately weigh each material according to the mass percentage in Table 5 above; 2) Weigh pure water and vitamin B 12 , pyridoxine hydrochloride, biotin, 1,2-pentanediol, and 1,2-propylene glycol are mixed and stirred until dissolved; then sodium citrate is added and stirred to dissolve to obtain phase A; 3) Evenly mix the weighed PEG-40 hydrogenated castor oil with lavender essential oil, rosemary essential oil and ginger essential oil, and then add butylene glycol to disperse to obtain phase B; 4) Pour phase A into phase B while stirring, and you will get a natural and pleasant fragrance, turmeric yellow, transparent and uniform, low viscosity, and vitamin B that has the function of hair growth. 12 Scalp care composition. Example

[0040] Table 6 Scalp care composition 3# formula

[0041] A vitamin B 12 The scalp care composition, the mass ratio of each component is shown in Table 6, and is prepared by the following method: 1) Accurately weigh each material according to the mass percentage in Table 6 above; 2) Weigh pure water and vitamin B 12 , nicotinamide, pyridoxine hydrochloride, 1,2-pentanediol, and 1,2-hexanediol, and stir until dissolved; then add sodium citrate, and stir to dissolve to obtain phase A; 3) Evenly mix the weighed PEG-40 hydrogenated castor oil with lavender essential oil, rosemary essential oil and ginger essential oil, and then add butylene glycol to disperse to obtain phase B; 4) Pour phase A into phase B while stirring, and you will get a natural and pleasant fragrance, turmeric yellow, transparent and uniform, low viscosity, and vitamin B that has the function of hair growth. 12 Scalp care composition. Example

[0042] Table 7 Scalp care composition 4# formula

[0043] A vitamin B 12 The scalp care composition, the mass ratio of each component is shown in Table 7, and is prepared by the following method: 1) Accurately weigh each material according to the mass percentage in Table 7 above; 2) Mix the weighed pure water and vitamin B 12 , niacinamide, glycerin, 1,2-pentanediol, 1,2-propylene glycol, and cocoeth-7, and stir until dissolved; then add citric acid and sodium citrate, and stir to dissolve to obtain phase A; 3) Evenly mix the weighed PEG-40 hydrogenated castor oil with lavender essential oil, rosemary essential oil and ginger essential oil, and then add 1,3-propylene glycol to disperse to obtain phase B; 4) Pour phase A into phase B while stirring, and you will get a natural and pleasant fragrance, turmeric yellow, transparent and uniform, low viscosity, and vitamin B that has the function of hair growth. 12 Scalp care composition.

[0044] Test Example 3: Stability Test In order to verify the stability effect, the prepared Examples 1 to 3 were placed at room temperature and away from light for 24 hours, and then the odor, transparency uniformity, stability, and whether stratification occurred in the soothing and moisturizing compositions were observed. The specific results are shown in Table 8.

[0045] Table 8 Stability test results of the soothing and moisturizing compositions prepared in Examples 1 to 3

[0046] Test Example 4: Actual Usage Evaluation After washing your hair, dry your scalp or use it on dry hair. Take an appropriate amount every day and wipe your scalp. Use your fingertips to gently massage your scalp to promote absorption. After 20 people have used the sample for 28 consecutive days, they will use a questionnaire to evaluate their feelings after using the hair care composition. Self-assessment scoring standard (5-point system): 1 point is very disagree, 2 points are somewhat disagree, 3 points are neither agree nor disagree, 4 points are relatively agree, and 5 points are very agree. The statistical score of >4 points is the valid number of people. The satisfaction calculation method is: satisfaction = (number of people scoring 4 points + number of people scoring 5 points / total number of people).

[0047] Table 9 Subjects’ self-assessment results satisfaction table

[0048] Actual evaluation shows that volunteers who continued to use the product for 28 days had obvious hair growth effects, scalp nourishment and good overall product satisfaction. The present invention can effectively solve the problems of poor hair quality and hair loss.

[0049] To sum up, the above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A kind of vitamin B 12 A scalp care composition having a hair growth promoting effect, characterized in that: Contains 0.0001%~0.0003% of vitamin B by mass 12 .

2. A kind of vitamin B 12 A scalp care composition having a hair growth promoting effect, characterized in that: It is composed of the following components in percentage by mass: 0.0001%~0.0003% vitamin B 12 , 5.0~20.0% compound active ingredients, 0.01~0.5% ginger essential oil, 0.02~0.50% lavender essential oil, 0.02~0.50% rosemary essential oil, 5.0~20.0% polyol, 0.02~2.0% pH adjuster, 0.1~2.0% solubilizer, and the balance is pure water; the compound active ingredient is one of panthenol, niacinamide, pyridoxine hydrochloride, biotin, or a mixture thereof in any mass ratio.

3. The composition according to claim 2 containing vitamin B 12 A scalp care composition having a hair growth promoting effect, characterized in that: The mass ratio of the rosemary essential oil, the lavender essential oil and the ginger essential oil is 3:12:

10.

4. The composition according to claim 2 containing vitamin B 12 A scalp care composition having a hair growth promoting effect, characterized in that: The polyol includes one of glycerol, 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, propylene glycol, butylene glycol, caprylyl glycol, ethylhexylglycerol, or a mixture thereof in any mass ratio.

5. The vitamin B-containing composition according to claim 2 12 A scalp care composition having a hair growth promoting effect, characterized in that: The pH regulator is one of citric acid, sodium citrate, arginine, tromethamine, aminomethyl propanol, or a mixture thereof in any mass ratio.

6. The vitamin B-containing composition according to claim 2 12 A scalp care composition having a hair growth promoting effect, characterized in that: The solubilizer is one of PEG-40 hydrogenated castor oil, cocoether-7, PPG-1-PEG-9 lauryl glycol ether, or a mixture thereof in any mass ratio.

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