Traditional Chinese medicine composition for treating skin furuncle, furuncle, carbuncle and carbuncle and preparation method thereof

Abstract

The invention belongs to the technical field of medical treatment, and particularly relates to a traditional Chinese medicine composition for treating skin furuncle, furuncle, carbuncle, and the traditional Chinese medicine composition for treating skin furuncle, furuncle, carbuncle comprises the following components: rosin, camphor, vermilion, borneol, venenum bufonis, and beeswax. The ointment is prepared from the following components in percentage by weight: 70 to 75 percent of rosin, 20 to 25 percent of camphor, 0 to 1.5 percent of vermilion, 1 to 1.5 percent of borneol, 0.5 to 1 percent of venenum bufonis and 2 to 2.5 percent of beewax. The six traditional Chinese medicinal materials are combined to achieve the effects of clearing away fire toxin, resisting bacteria and relieving swelling and pain; tests show that the traditional Chinese medicinal material mixture can effectively reduce release of local inflammation mediator of abscess, achieve the effects of expelling toxin and pus, removing slough and dispersing blood stasis, and promoting tissue regeneration and healing sore, improve the micro-ecology of abscess skin, and can directly fade after mild bacterial infection of skin without a sore surface; for abscesses with formed sore surfaces, pus discharge can be accelerated, and recovery and healing of the sore surfaces are promoted.

Description

Technical Field

[0001] The invention relates to the field of medical technology, in particular to a traditional Chinese medicine composition for treating skin furuncle, boil, carbuncle and carbuncle and a preparation method thereof. Background Art

[0002] Furuncle, carbuncle, ulcer and carbuncle are names for a class of surgical diseases in traditional Chinese medicine. They are diseases that occur at different locations and depths on the body surface. Such diseases were first recorded in the Yellow Emperor's Internal Classic, in which the Suwen·Original Theory of the Qi Tong Tian states: "When sweat comes out and moisture appears, acne and carbuncle will appear. The change of rich food and rich food will cause large dings on the feet, and the pain will be like holding emptiness. Exhaustion and sweating in the wind will cause thin and cold ulcers, and depression will cause acne." The Lingshu·Carbuncle states: "When the cold evil invades the meridians, the blood will cry, and when the blood cries, the meridians will be blocked. When the meridians are blocked, the defensive Qi will return and cannot be restored, so carbuncle will appear. Cold turns into heat, and when the heat prevails, the flesh will rot, and when the flesh rots, it will become pus." The pathogenesis and clinical manifestations of such diseases are also recorded in the Various Diseases and Origin Theory. The symptoms of carbuncle are: "The swelling is one inch long. When it reaches two inches, it is called a furuncle. It is also like carbuncle fever. After a long time, pus will form. When the pus and blood are squeezed out, it will heal. It is also caused by the wind and cold air invading the skin, causing blood and qi to stagnate. In all carbuncle and carbuncle, the pus and blood cannot be squeezed out, and the wound will close. The foul juice is inside. Although it is healed, it can eventually break out again and become a fistula. "; Carbuncle: "Carbuncle will occur when the five internal organs are not in harmony. It is also caused by the cold invading the skin, damaging the blood and qi, causing blood and qi to stagnate and stagnate. It is similar to carbuncle in general, but the difference is that the skin on it is like the skin of a cow's collar but hard. Carbuncle is superficial, while carbuncle is deep. As for the transformation into pus and ulcer, it is more serious than carbuncle, injuring bones and rotting tendons, and even leading to death."

[0003] Traditional Chinese medicine believes that this disease is mostly caused by the six external evils (heat and dampness, fire poison, and heat poison), which contain dampness, heat, and fire poison inside, resulting in disharmony between the Ying and Wei, leading to poor Qi movement, obstruction of Qi and blood flow, and fumigation of the skin. "Lingshu·Carbuncle" records that "when the Ying and Wei are retained in the meridians, the blood will cry and not flow, and if it does not flow, the Wei Qi will follow and not flow, and it will be blocked and cannot flow, so it is hot. If the heat does not stop, the heat will prevail and the flesh will rot, and the rotten flesh will become pus." Therefore, all swellings and itches are heat syndromes, especially fire poison and heat poison. In traditional Chinese medicine treatment, "clearing heat and detoxifying, reducing swelling and relieving pain, removing rot and regenerating flesh" are the main treatments.

[0004] According to the research of Western medicine on bacterial skin diseases, human skin is covered with about one thousand kinds of bacteria. The bacteria on the skin are of various types, including aerobic cocci, aerobic and anaerobic rod-shaped cocci, Gram-negative bacteria, etc., including Staphylococcus, Pseudomonas aeruginosa, Candida albicans, Staphylococcus epidermidis, etc. According to the classification and characteristics of bacteria on the skin, some bacteria will complete the complete growth cycle and reproduction process on the skin. Such bacteria are usually harmless or even beneficial to the human body; but some bacteria are temporarily living on the skin and may disappear within a certain period, such as Staphylococcus aureus and Streptococcus. Such bacteria may come from the external environment or from other parts of the human body. These bacteria live on the skin, coexist with other microorganisms on the skin, and form a microecology and achieve ecological balance. Microorganisms help the host prevent skin infections by producing fatty acids that have a killing effect on many bacteria. However, under the influence of certain internal and external factors, the delicate balance between the host and the microorganisms is destroyed, which may lead to skin diseases or infections and form coccal skin diseases. Furuncles, carbuncles, ulcers, and carbuncles are mostly caused by infections with Staphylococcus aureus and a small amount of Staphylococcus albicans. In Western medicine, they are mostly treated with antibiotics and antiviral drugs. For severe suppuration, surgical drainage and removal of necrotic tissue are required. Summary of the invention

[0005] The object of the present invention is to provide a Chinese medicine composition for treating skin furuncle, carbuncle, ulcer and carbuncle and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical scheme: a Chinese medicinal composition for treating skin furuncle, furuncle, carbuncle and carbuncle, the Chinese medicinal composition for treating skin furuncle, furuncle, carbuncle and carbuncle comprises the following components:

[0007] Rosin, camphor, vermilion, borneol, toad venom, beeswax, according to mass proportion, the specific proportion of each component is as follows:

[0008] Rosin 70-75%, camphor 20-25%, vermilion 0-1.5%, borneol 1-1.5%, toad venom 0.5-1%, beeswax 2-2.5%.

[0009] Preferably, the proportions of the rosin, camphor, vermilion, borneol, toad venom and beeswax by weight are as follows: rosin 71.94%, camphor 21.58%, vermilion 1.44%, borneol 1.44%, toad venom 0.72% and beeswax 2.16%.

[0010] Preferably, the proportions of the rosin, camphor, vermilion, borneol, toad venom and beeswax by weight are as follows: rosin 74.5%, camphor 21%, vermilion 1%, borneol 1%, toad venom 0.5% and beeswax 2%.

[0011] Preferably, the proportions of the rosin, camphor, vermilion, borneol, toad venom and beeswax by weight are as follows: rosin 70%, camphor 23.5%, vermilion 1.5%, borneol 1.5%, toad venom 1% and beeswax 2.5%.

[0012] A method for preparing a Chinese medicinal composition for treating skin furuncle, boil, carbuncle and carbuncle, the method for preparing a Chinese medicinal composition for treating skin furuncle, boil, carbuncle and carbuncle;

[0013] The preparation method of the Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle is as follows:

[0014] Step 1: Grind camphor, vermilion, borneol and toad venom into powder respectively;

[0015] Step 2: Place the rosin in a clean cooking container and melt it over a low heat. The temperature should not be too high. After all the rosin is melted, boil for 5 minutes, then remove from the heat and cool to about 80 degrees.

[0016] Step 3: Add camphor and vermilion in turn, adding in small amounts several times, stirring slowly until completely dissolved;

[0017] Step 4: Add toad venom and beeswax in sequence, and stir slowly until they are completely dissolved to form the plaster of the present invention;

[0018] Step 5: Apply the plaster on the kraft paper and let it cool.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The combination of the six Chinese medicinal materials has the effects of clearing away heat toxins, resisting bacteria, reducing swelling and relieving pain. Experiments have shown that the Chinese medicinal material mixture of the present invention can effectively reduce the release of local inflammatory mediators of abscesses, achieve the effects of expelling toxins and draining pus, removing rotten flesh and removing blood stasis, promoting tissue regeneration and healing sores, and improve the microecology of the skin of abscesses. Mild bacterial infections of the skin that have not formed sores can be directly eliminated after medication; for abscesses that have already formed sores, it can accelerate the drainage of pus and promote the recovery and healing of the sore surface. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a sample completion diagram of the present invention;

[0022] Figure 2 A graph showing absorbance detection results;

[0023] Figure 3 Figure 2 is the absorbance detection result. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0026] See also Figure 1-3 The present invention provides a technical solution: a traditional Chinese medicine composition for treating skin furuncle, furuncle, carbuncle and carbuncle, the traditional Chinese medicine composition for treating skin furuncle, furuncle, carbuncle and carbuncle comprising the following components: rosin, camphor, vermilion, borneol, toad venom and beeswax, wherein the proportion of each component by weight is as follows: rosin 70-75%, camphor 20-25%, vermilion 0-1.5%, borneol 1-1.5%, toad venom 0.5-1% and beeswax 2-2.5%.

[0027] Rosin functions are drying dampness, removing toxins, promoting tissue regeneration, and relieving pain. It is mainly used to treat carbuncle, malignant ulcer, and has the effects of aromatic penetrating tendons and bones, warming and unblocking meridians, and can penetrate into the skin, dissolve turbid air, and dispel wind and cold. The present invention uses rosin as the main matrix of plaster, which helps the drug components to release and penetrate the skin, and is solid at room temperature, has good stability, has good viscosity and ductility when used, can ensure that the plaster is close to the skin, and will not cause skin damage when removed. In the production process, it can be melted by a temperature control device and mixed fully with other drug components, so that it is easy to process and produce different plaster shapes.

[0028] Rosin is the matrix of this scheme and accounts for a relatively high proportion. According to the needs of use, the rosin ratio of this scheme can be adjusted to reduce the rosin ratio and retain the pharmacological effects of rosin, and replace it with an emulsion-type matrix.

[0029] Camphor has the functions of opening the mind, eliminating filth, reducing swelling and relieving pain. It is mainly used to treat fever, coma, scabies, stubborn ringworm, and ecthyma, and has the effect of warming and dispersing pain. Camphor has a mild irritation to the skin, can play a mild anesthesia in external use, and can effectively enhance the analgesic effect when used in combination with rosin, and can improve the warming and unblocking effect of rosin, promote the absorption of other drug ingredients by the skin, help to dissipate local inflammation, treat carbuncle swelling, ulceration, pus and other symptoms. The present invention can further enhance the adhesion of plasters by adding a certain proportion of camphor, improve the ductility of plasters, and make it easier to apply and remove.

[0030] Toad venom has the functions of opening the orifices, detoxifying, reducing swelling and relieving pain. It is mainly used to treat carbuncle, malignant ulcer, and toothache caused by carbuncle. It has good detoxification, detumescence, anti-inflammatory and analgesic effects. The indole alkaloids in toad venom have a good anti-inflammatory effect. The sterol substances in toad venom can inhibit vascular permeability, have a good inhibitory effect on Staphylococcus aureus and type alpha hemolytic streptococci, and have obvious and rapid antibacterial effects. "Shennong's Herbal Classic" records: "Toad venom, its taste is pungent and sweet, and its temperature is dispersing. It can dissipate all wind-fire depression, high heat carbuncle, and is a magical medicine for removing carbuncle and dispersing poison." Toad venom and borneol are often used in combination in external preparations, which can effectively enhance its detoxification and analgesic effects, and are used to treat carbuncle, malignant ulcer, carbuncle, and back.

[0031] Silver Vermilion has the functions of attacking poison, drying dampness, and killing insects. It is mainly used to treat carbuncle, swelling, toxicity, malignant sore, ecthyma, and scabies. The "Compendium of Materia Medica" records that "Silver Vermilion and light powder have slightly different properties in terms of cold and warm, but their main treatments are the same. It is more dry and dispersing, and is good at external treatment, not suitable for internal use." Silver Vermilion is also often used in the prescriptions for treating carbuncle, swelling, toxicity, etc. recorded in "Yi Zong Jin Jian", "Jiu Ji Fang" and "Jian Ji Liang Fang".

[0032] Borneol has the functions of opening all the orifices, dispersing stagnant fire, reducing swelling and relieving pain. It is mainly used to treat red and swollen eyes, swollen and painful sores, and ulcers that do not heal. In a certain concentration, borneol has antibacterial and anti-inflammatory effects in external use. It has antibacterial effects on Staphylococcus aureus, beta-hemolytic Streptococcus, Pneumococcus and Escherichia coli, and has an inhibitory effect on the verification process of fluid exudation and tissue edema.

[0033] Beeswax is not used for its pharmacological effects in this solution. Its main function is to shape the plaster at room temperature and facilitate storage. According to the needs of use, this solution can be made into an ointment, so as to appropriately adjust the proportion of beeswax.

[0034] The following three groups of ratios can be used:

[0035] The first group of ratios:

[0036] The specific proportions of the rosin, camphor, vermilion, borneol, toad venom and beeswax by weight are: rosin 71.94%, camphor 21.58%, vermilion 1.44%, borneol 1.44%, toad venom 0.72% and beeswax 2.16%.

[0037] The second group of ratios:

[0038] The specific proportions of the rosin, camphor, vermilion, borneol, toad venom and beeswax by weight are: rosin 74.5%, camphor 21%, vermilion 1%, borneol 1%, toad venom 0.5% and beeswax 2%.

[0039] The third group ratio method:

[0040] The specific proportions of the rosin, camphor, vermilion, borneol, toad venom and beeswax by weight are: 70% rosin, 23.5% camphor, 1.5% vermilion, 1.5% borneol, 1% toad venom and 2.5% beeswax.

[0041] A method for preparing a Chinese medicinal composition for treating skin furuncle, boil, carbuncle and carbuncle, the method for preparing a Chinese medicinal composition for treating skin furuncle, boil, carbuncle and carbuncle;

[0042] The preparation method of the Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle is as follows:

[0043] Step 1: Grind camphor, vermilion, borneol and toad venom into powder respectively;

[0044] Step 2: Place the rosin in a clean cooking container and melt it over a low heat. The temperature should not be too high. After all the rosin is melted, boil for 5 minutes, then remove from the heat and cool to about 80 degrees.

[0045] Step 3: Add camphor and vermilion in turn, adding in small amounts several times, stirring slowly until completely dissolved;

[0046] Step 4: Add toad venom and beeswax in sequence, and stir slowly until they are completely dissolved to form the plaster of the present invention;

[0047] Step 5: Apply the plaster on the kraft paper and let it cool.

[0048] A drug (hereinafter referred to as MH) was prepared based on the above-described scheme, and the following experiments were performed on the drug:

[0049] Experimental materials and instruments

[0050] Main experimental consumables and instruments

[0051] Bacterial strains: purchased from Guangdong Microbiological Culture Collection Center, strain number: GDMCC1.1220 (i.e. ATCC6538) (skin furuncles, boils, carbuncles, and carbuncles are mainly infected by Staphylococcus aureus and other strains);

[0052] Culture medium: MH medium;

[0053] Test sample: Chinese medicine extract, provided by the customer (600 mg / mL);

[0054] Experimental consumables: 96-well plate, 1.5 mL centrifuge tube, 500 mL conical flask, glass test tube;

[0055] McFarland turbidimeter;

[0056] Micropipettes;

[0057] Biochemical incubator;

[0058] Alcohol lamp;

[0059] Experimental methods

[0060] Preparation of culture medium

[0061] Accurately weigh 4.2 g of MH (the drug prepared according to the formula of this scheme) powder, pour it into a clean conical flask containing 100 mL of ultrapure water, stir with a glass rod to fully dissolve, then add ultrapure water to make up to 200 mL, sterilize with high-pressure steam at 121°C for 20 min, and set aside after cooling.

[0062] Bacterial recovery

[0063] A frozen strain of Staphylococcus aureus was inoculated into MH medium, cultured at 37°C for 18-24h, and passed for three generations.

[0064] Bacterial solution concentration adjustment

[0065] The bacteria to be used were cultured to the logarithmic growth phase, and the concentration of the bacterial solution was adjusted to 0.5 McFarland concentration (about 1.5×108) using a McFarland turbidimeter, and then diluted to 1.5×105 with culture medium at 1:1000, and the bacterial inoculation was completed within 20 minutes.

[0066] Microdilution method for MIC detection

[0067] Preparation of gradient concentrations of test drugs

[0068] ① Add 100 μL MH medium to wells 1-6, add 200 μL medium to wells H4-6, and wells H4-6 serve as blank medium controls.

[0069] ② Add 200μL of 480mg / mL Chinese herbal medicine extract to wells A1-3, mix well, aspirate 100μL and add to wells B1-3, mix well, aspirate 100μL of drug from wells B1-3 and MH medium, aspirate 100μL of drug from wells B1-3 and mix well with MH medium in wells C1-3, and so on, 2-fold gradient dilution until wells G1-3, aspirate 100μL of liquid from well 7 and discard.

[0070] ③ Add alcohol of the same concentration as that of the Chinese medicine extract to A4-6 as a control, and dilute it in the same multiple ratio.

[0071] Add bacterial suspension

[0072] Add 100 μL of 1.5×105 bacterial suspension to wells 1-6.

[0073] Note: The MIC was tested by microdilution method with 3 replicates for each concentration gradient.

[0074] Ethanol Control Setup

[0075] 30% ethanol was diluted twice as above, and 100 μL of 1.5×105 bacterial suspension was added. Each concentration gradient was repeated 3 times.

[0076] Absorbance detection

[0077] After adding the bacterial suspension, the absorbance was immediately measured at OD600nm.

[0078] Since the drug itself has color and drug precipitation appears in the sample well after the addition, the absorbance is detected at OD600nm after incubation at 37°C for 24h.

[0079] Experimental Results

[0080] like Figure 1 As shown, H1-3 is a negative control of bacterial culture, and the culture medium is turbid, indicating that the bacteria grow well. H4-6 is a culture medium control, and the culture medium is clear and transparent, indicating that there is no bacterial contamination and the operation is good.

[0081] like Figure 2 As shown, the drug itself has color and solubility issues, which have an impact on absorbance. The 30% alcohol dilution control group has no effect on absorbance. The absorbance readings in columns 1-3 are immediately detected after adding the drug, which is due to the crystallization of the Chinese medicine extract in the MH culture medium. The readings in columns 4-6 are the culture base.

[0082] like Figure 3 As shown, although the drug itself has an impact on absorbance due to color and solubility issues, the absorbance of the sample group changed significantly after Staphylococcus aureus was added, indicating that the drug has an effect on bacteria.

[0083] In the alcohol dilution group AG 4-6 wells, the absorbance did not change much, indicating that the 30%-2.5% alcohol concentration had no bactericidal effect on Staphylococcus aureus.

[0084] The absorbance of the Chinese herbal medicine extract group AF 1-3 columns decreased with the change of drug concentration. When the concentration reached 10 mg / mL, the bacterial solution became clear and the absorbance was the lowest, close to the absorbance of the negative control well. When the drug concentration was lower than 5 mg / mL (G1-3), the drug concentration was not enough to kill Staphylococcus aureus, and the reagent became turbid, causing the absorbance to begin to increase. The results of this experiment suggest that the lowest concentration of the drug for Staphylococcus aureus is 10 mg / mL (careful observation revealed that there was almost no drug crystal precipitation at the bottom of the test well).

[0085] Note: Figure 1 In the figure, columns 1-3 are experimental groups (A1-3-G1-3), columns 4-6 are ethanol controls (A4-6-F4-6), H4-6 are blank controls, and H1-3 are negative controls.

[0086] Figure 2 For immediate detection after sample addition, Figure 3 After adding the sample, incubate in a 37°C biochemical box for 24 hours for detection.

[0087] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention; therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is limited by the attached claims rather than the above description. Therefore, it is intended to include all changes within the meaning and scope of the equivalent elements of the claims in the present invention, and any figure marks in the claims should not be regarded as limiting the claims involved.

[0088] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle, characterized in that: The Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle comprises the following components: Rosin, camphor, vermilion, borneol, toad venom, beeswax, according to mass proportion, the specific proportion of each component is as follows: Rosin 70-75%, camphor 20-25%, vermilion 0-1.5%, borneol 1-1.5%, toad venom 0.5-1%, beeswax 2-2.5%.

2. A Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle according to claim 1, characterized in that: The specific proportions of the rosin, camphor, vermilion, borneol, toad venom and beeswax by weight are: rosin 71.94%, camphor 21.58%, vermilion 1.44%, borneol 1.44%, toad venom 0.72% and beeswax 2.16%.

3. A Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle according to claim 1, characterized in that: The specific proportions of the rosin, camphor, vermilion, borneol, toad venom and beeswax by weight are: rosin 74.5%, camphor 21%, vermilion 1%, borneol 1%, toad venom 0.5% and beeswax 2%.

4. A Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle according to claim 1, characterized in that: The specific proportions of the rosin, camphor, vermilion, borneol, toad venom and beeswax by weight are: 70% rosin, 23.5% camphor, 1.5% vermilion, 1.5% borneol, 1% toad venom and 2.5% beeswax.

5. A method for preparing a Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle, characterized in that: The preparation method is directed to the Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle as claimed in any one of claims 1 to 4; The preparation method of the Chinese medicinal composition for treating skin furuncle, carbuncle, ulcer and carbuncle is as follows: Step 1: Grind camphor, vermilion, borneol and toad venom into powder respectively; Step 2: Place the rosin in a clean cooking container and melt it over a low heat. The temperature should not be too high. After all the rosin is melted, boil for 5 minutes, then remove from the heat and cool to about 80 degrees. Step 3: Add camphor and vermilion in turn, adding in small amounts several times, stirring slowly until completely dissolved; Step 4: Add toad venom and beeswax in sequence, and stir slowly until they are completely dissolved to form the plaster of the present invention; Step 5: Apply the plaster on the kraft paper and let it cool.

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