Traditional Chinese medicine ointment rich in organic selenium and small-molecular oligopeptide as well as preparation method and application of traditional Chinese medicine ointment

Abstract

The invention relates to a traditional Chinese medicine ointment rich in organic selenium and small molecule oligopeptide and a preparation method and application thereof. The traditional Chinese medicine ointment comprises the following components in percentage by mass: 50-70% of a traditional Chinese medicine extract; 0.05 to 0.2 percent of organic selenium; 5-15% of small molecule peptide; and 10-20% of an auxiliary material. The traditional Chinese medicine extract is prepared from the following raw materials in parts by weight: 30g of astragalus membranaceus, 25g of poria cocos, 15g of angelica sinensis and 10g of fructus lycii. The organic selenium is selected from one of selenium-enriched yeast or selenocystine, the small molecule peptide is selected from one of collagen peptide or soybean peptide, and the molecular weight of the small molecule peptide is 1t; the auxiliary material is fructo-oligosaccharide, the small molecule peptide is subjected to sodium alginate-chitosan microencapsulation treatment, and the encapsulation efficiency is greater than or equal to 85%. By constructing a'traditional Chinese medicine-selenium-peptide 'ternary synergistic system and by virtue of an innovative process, active ingredients of medicinal materials are efficiently reserved, the immunity, oxidation resistance and metabolism functions are synergistically improved, and the selenium-peptide health-care food is suitable for conditioning sub-health states such as prediabetes and chronic fatigue syndrome.

Description

Technical Field

[0001] The invention relates to the technical field of the intersection of traditional Chinese medicine compound preparations and functional foods, and in particular to a traditional Chinese medicine paste prescription rich in organic selenium and small molecule short peptides, and a preparation method and application thereof. Background Art

[0002] Chinese medicine paste is a unique dosage form in traditional Chinese medicine. It belongs to the category of traditional pastes and has a long history and rich clinical applications. Chinese medicine paste (also known as "paste") is based on the theory of traditional Chinese medicine. It is a semi-fluid or solid preparation made by repeatedly decocting and concentrating a variety of Chinese medicinal materials, adding auxiliary materials such as honey, donkey-hide gelatin, and crystal sugar. It has a thick texture and a sweet taste. It is usually used for long-term conditioning or nourishment.

[0003] In the existing technology, such as the Chinese patent with publication number CN104621434A, it discloses a formula of a dietary therapy qi-invigorating and blood-nourishing paste, which is composed of 1.5 parts of angelica, 1.5 parts of astragalus, 2 parts of donkey-hide gelatin, 1 part of red date slices, 2 parts of black sesame, 1 part of walnut kernels, 1 part of rock sugar, and an appropriate amount of rice wine. When making it, first mix the angelica, astragalus, donkey-hide gelatin, red date slices, black sesame, and rock sugar into powder, then mix them with walnut kernels with rice wine to form a semi-wet paste, put them into a flat-bottomed container, and flatten the top, then put the container into a steamer and steam it in water, requiring a high temperature of more than 100°C, and steam for about 1.5 hours. At this time, the water in the paste is basically steamed out, take it out of the cage and dry it, and use a knife to cut the paste into matchbox size for consumption. Two to three pieces can be eaten every day. The paste can nourish blood, moisturize dryness, replenish qi and promote blood circulation, especially for weak constitution and the recovery of chronic diseases. It has a good recovery effect.

[0004] Another example is the Chinese patent with publication number CN108066712A, which discloses a Chinese medicine composition for invigorating qi and nourishing blood, the raw materials of which include: ginseng, polygonatum, red dates, black dates, sour dates, black sesame, mulberry, raspberry, rose, pine pollen, daidai flower, bergamot, cassia seed, light black beans, orange peel, cinnamon, malt, charred hawthorn, almond, lotus seed, black-necked snake, agkistrodon, turmeric, galangal, citron, lily and donkey-hide gelatin. The present invention also provides a prescription for invigorating qi and nourishing blood prepared with the above-mentioned Chinese medicine composition for invigorating qi and nourishing blood. The Chinese medicine composition for invigorating qi and nourishing blood of the present invention has a rigorous formula and accurate compatibility, has the effects of invigorating qi and nourishing blood, regulating the balance of qi and blood yin and yang, resisting fatigue, and improving immunity, and can be used to prepare products for improving qi and blood deficiency. The two Chinese medicine pastes are similar to traditional Chinese medicine pastes. They are mainly based on tonic herbs (such as donkey-hide gelatin and ginseng), which are concentrated by decoction to form a semi-fluid dosage form, which has the effect of nourishing qi and blood and regulating internal organs. However, they have the following defects: Singleness: The above pastes only target a single syndrome (such as qi deficiency and blood deficiency) and lack the ability to intervene in multiple targets of modern sub-health conditions (such as metabolic syndrome and chronic fatigue). Insufficient retention of active ingredients: Traditional high-temperature boiling process (usually above 100°C) easily destroys heat-sensitive ingredients (such as polyphenols and peptides), resulting in reduced bioavailability. Summary of the invention

[0005] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a traditional Chinese medicine paste recipe rich in organic selenium and small molecule short peptides, and a preparation method and application thereof. It constructs a "traditional Chinese medicine-selenium-peptide" ternary synergistic system and achieves efficient retention of the active ingredients of medicinal materials through innovative processes, synergistically improves the immune, antioxidant and metabolic functions, and is suitable for the conditioning of sub-health conditions such as prediabetes and chronic fatigue syndrome.

[0006] The above-mentioned object of the present invention is achieved through the following technical solutions: A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides, comprising the following components by mass percentage: Chinese herbal medicine extract: 50-70%; Organic selenium: 0.05-0.2%; Small molecule peptides: 5-15%; Auxiliary materials: 10-20%.

[0007] As a further technical solution of the present invention: the Chinese medicine extract includes a preparation made from the following raw materials in parts by weight: 30g of Astragalus, 25g of Poria, 15g of Angelica, and 10g of Lycium barbarum.

[0008] As a further technical solution of the present invention: the organic selenium is selected from one of selenium-enriched yeast and selenocystine.

[0009] As a further technical solution of the present invention: the small molecule peptide is selected from collagen peptide or soybean peptide.

[0010] As a further technical solution of the present invention: the molecular weight of the small molecule peptide is <1000Da.

[0011] As a further technical solution of the present invention: the auxiliary material is oligofructose.

[0012] As a further technical solution of the present invention: the small molecule peptide is processed by sodium alginate-chitosan microencapsulation, and the encapsulation rate is ≥85%.

[0013] The method for preparing the above-mentioned Chinese medicine paste rich in organic selenium and small molecule short peptides comprises the following steps: Step 1: Mixing, adding water to the Chinese herbal medicine extract for decoction, decoction twice (1.5 hours for the first time and 1 hour for the second time) to extract water-soluble polysaccharides (such as astragalus polysaccharide) and saponins (such as pachymic acid); Step 2, enzymatic hydrolysis, adding 0.5-1.5% w / w cellulase to the decocted medicinal residue, enzymatic hydrolysis at 45°C for 2 hours to release fat-soluble components (such as angelica lactone), and then concentrating to form a concentrate; Step 3: Microencapsulation of the peptide, using sodium alginate-chitosan composite wall material, spray drying, air inlet temperature 160°C, air outlet temperature 80°C, to prepare a small molecule peptide, with an encapsulation rate ≥ 85%, and then adding the small molecule peptide to the concentrate, stirring and mixing at 60°C; Step 4: Add selenium at low temperature, concentrate in a vacuum low temperature environment (temperature ≤ 60°C), add organic selenium and auxiliary materials to the concentrate for homogenization to avoid oxidative inactivation of selenium; Step 5, freeze drying, pre-freeze to -40°C, sublimation drying for 24 hours, moisture ≤5%, to obtain a Chinese medicine paste recipe.

[0014] As a further technical solution of the present invention: in step 4, the vacuum low-temperature environment is: pressure -0.08MPa, temperature 50-55°C, concentrated to a relative density of 1.20-1.30.

[0015] For example, the above-mentioned Chinese medicine paste recipe rich in organic selenium and small molecule short peptides can be used in any one of the following aspects: conditioning of sub-healthy conditions, prevention and treatment of chronic diseases, and support for postoperative or postpartum rehabilitation.

[0016] In summary, the present invention includes at least one of the following beneficial technical effects: The present invention discloses a Chinese medicine paste rich in organic selenium and small molecule short peptides, and a preparation method and application thereof. The paste is a composite paste that integrates active ingredients of Chinese medicine, organic selenium and small molecule peptides. Among them, organic selenium (such as selenocystine) can activate glutathione peroxidase (GSH-Px) and enhance antioxidant capacity; small molecule peptides (molecular weight <1000Da) have the function of promoting cell repair and regulating immunity. The activity of medicinal materials is maximized by staged extraction technology, and the peptide microencapsulation and selenium low-temperature addition process are combined to achieve a selenium retention rate of ≥95% and a peptide activity retention rate of ≥90%. The paste can synergistically improve immune, antioxidant and metabolic functions by activating the TLR4 / NF-κB pathway, enhancing GSH-Px activity and promoting IL-2 secretion, and is suitable for conditioning sub-health conditions such as prediabetes and chronic fatigue syndrome. The core of the present invention is to construct a ternary synergistic system of "Chinese medicine-selenium-peptide" and achieve efficient retention of active ingredients through innovative processes. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the embodiments of the present application; obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application without making creative work are within the scope of protection of the present application. Example

[0018] A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides, comprising the following components by mass percentage: Chinese herbal medicine extract: 65%; Organic selenium: 0.1%; Small molecule peptides: 15%; Auxiliary materials: 19.9%.

[0019] The Chinese medicine extract includes a preparation made from the following raw materials by weight: 30g of Astragalus, 25g of Poria, 15g of Angelica, and 10g of Lycium barbarum. Organic selenium is selected from one of selenium-enriched yeast or selenocysteine. In this embodiment, organic selenium is preferably selenium-enriched yeast. The small molecule peptide is selected from one of collagen peptide or soybean peptide. In this embodiment, the small molecule peptide is preferably collagen peptide, the molecular weight of the small molecule peptide is <1000Da, and the auxiliary material is oligofructose. The small molecule peptide is microencapsulated by sodium alginate-chitosan, and the encapsulation rate is ≥85%.

[0020] The method for preparing the above-mentioned Chinese medicine paste rich in organic selenium and small molecule short peptides comprises the following steps: Step 1: Mixing, adding water to the Chinese herbal medicine extract for decoction, decoction twice (1.5 hours for the first time and 1 hour for the second time) to extract water-soluble polysaccharides (such as astragalus polysaccharide) and saponins (such as pachymic acid); Step 2, enzymatic hydrolysis, adding 0.5-1.5% w / w cellulase to the decocted medicinal residue, enzymatic hydrolysis at 45°C for 2 hours to release fat-soluble components (such as angelica lactone), and then concentrating to form a concentrate; Step 3: Microencapsulation of the peptide, using sodium alginate-chitosan composite wall material, spray drying, air inlet temperature 160°C, air outlet temperature 80°C, to prepare a small molecule peptide, with an encapsulation rate ≥ 85%, and then adding the small molecule peptide to the concentrate, stirring and mixing at 60°C; Step 4: Add selenium at low temperature, concentrate in a vacuum low temperature environment (temperature ≤ 60°C), the vacuum low temperature environment is: pressure -0.08MPa, temperature 50-55°C, concentrate to a relative density of 1.20-1.30, add organic selenium and auxiliary materials to the concentrate for homogenization to avoid selenium oxidation inactivation; Step 5, freeze drying, pre-freeze to -40°C, sublimation drying for 24 hours, moisture ≤5%, to obtain a Chinese medicine paste recipe. Example

[0021] A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides, comprising the following components by mass percentage: Chinese herbal medicine extract: 68%; Organic selenium: 0.05%; Small molecule peptides: 11.95%; Auxiliary materials: 20%.

[0022] The Chinese medicine extract includes a preparation made from the following raw materials by weight: 30g of Astragalus, 25g of Poria, 15g of Angelica, and 10g of Lycium barbarum. Organic selenium is selected from one of selenium-enriched yeast or selenocysteine. In this embodiment, organic selenium is preferably selenium-enriched yeast. The small molecule peptide is selected from one of collagen peptide or soybean peptide. In this embodiment, the small molecule peptide is preferably collagen peptide, the molecular weight of the small molecule peptide is <1000Da, and the auxiliary material is oligofructose. The small molecule peptide is microencapsulated by sodium alginate-chitosan, and the encapsulation rate is ≥85%.

[0023] The method for preparing the above-mentioned Chinese medicine paste rich in organic selenium and small molecule short peptides comprises the following steps: Step 1: Mixing, adding water to the Chinese herbal medicine extract for decoction, decoction twice (1.5 hours for the first time and 1 hour for the second time) to extract water-soluble polysaccharides (such as astragalus polysaccharide) and saponins (such as pachymic acid); Step 2, enzymatic hydrolysis, adding 0.5-1.5% w / w cellulase to the decocted medicinal residue, enzymatic hydrolysis at 45°C for 2 hours to release fat-soluble components (such as angelica lactone), and then concentrating to form a concentrate; Step 3: Microencapsulation of the peptide, using sodium alginate-chitosan composite wall material, spray drying, air inlet temperature 160°C, air outlet temperature 80°C, to prepare a small molecule peptide, with an encapsulation rate ≥ 85%, and then adding the small molecule peptide to the concentrate, stirring and mixing at 60°C; Step 4: Add selenium at low temperature, concentrate in a vacuum low temperature environment (temperature ≤ 60°C), the vacuum low temperature environment is: pressure -0.08MPa, temperature 50-55°C, concentrate to a relative density of 1.20-1.30, add organic selenium and auxiliary materials to the concentrate for homogenization to avoid selenium oxidation inactivation; Step 5, freeze drying, pre-freeze to -40°C, sublimation drying for 24 hours, moisture ≤5%, to obtain a Chinese medicine paste recipe. Example

[0024] A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides, comprising the following components by mass percentage: Chinese herbal medicine extract: 70%; Organic selenium: 0.2%; Small molecule peptides: 15%; Auxiliary materials: 14.8%.

[0025] The Chinese medicine extract includes a preparation made from the following raw materials by weight: 30g of Astragalus, 25g of Poria, 15g of Angelica, and 10g of Lycium barbarum. Organic selenium is selected from one of selenium-enriched yeast or selenocysteine. In this embodiment, organic selenium is preferably selenium-enriched yeast. The small molecule peptide is selected from one of collagen peptide or soybean peptide. In this embodiment, the small molecule peptide is preferably collagen peptide, the molecular weight of the small molecule peptide is <1000Da, and the auxiliary material is oligofructose. The small molecule peptide is microencapsulated by sodium alginate-chitosan, and the encapsulation rate is ≥85%.

[0026] The method for preparing the above-mentioned Chinese medicine paste rich in organic selenium and small molecule short peptides comprises the following steps: Step 1: Mixing, adding water to the Chinese herbal medicine extract for decoction, decoction twice (1.5 hours for the first time and 1 hour for the second time) to extract water-soluble polysaccharides (such as astragalus polysaccharide) and saponins (such as pachymic acid); Step 2, enzymatic hydrolysis, adding 0.5-1.5% w / w cellulase to the decocted medicinal residue, enzymatic hydrolysis at 45°C for 2 hours to release fat-soluble components (such as angelica lactone), and then concentrating to form a concentrate; Step 3: Microencapsulation of the peptide, using sodium alginate-chitosan composite wall material, spray drying, air inlet temperature 160°C, air outlet temperature 80°C, to prepare a small molecule peptide, with an encapsulation rate ≥ 85%, and then adding the small molecule peptide to the concentrate, stirring and mixing at 60°C; Step 4: Add selenium at low temperature, concentrate in a vacuum low temperature environment (temperature ≤ 60°C), the vacuum low temperature environment is: pressure -0.08MPa, temperature 50-55°C, concentrate to a relative density of 1.20-1.30, add organic selenium and auxiliary materials to the concentrate for homogenization to avoid selenium oxidation inactivation; Step 5, freeze drying, pre-freeze to -40°C, sublimation drying for 24 hours, moisture ≤5%, to obtain a Chinese medicine paste recipe.

[0027] Animal experiment example: Animal experiments (verification of immune regulation and metabolic improvement): Subjects: Healthy male BALB / c mice (immunomodulation experiment, n = 10 per group); Prediabetic model rats (metabolic improvement experiment, n=8 per group); Experimental groups: Control group 1: traditional paste formula (without selenium and peptide); Control group 2: paste formula + selenium (without peptide); Control group 3: paste formula + peptide (without selenium); Experimental group: paste formula + selenium + peptide (preferably the preparation method and formula of Example 3 of the present invention).

[0028] Experimental steps: Step S1, adaptive feeding: the animals were adaptively fed under a standard environment (temperature 25±2°C, humidity 50±10%) for 7 days.

[0029] Step S2, drug administration: oral administration once a day, the dose is 0.5 g / kg (mice) or 1.0 g / kg (rat), for 28 consecutive days.

[0030] The ointment was diluted with an equal volume of saline in both the control group and the experimental group.

[0031] Index detection: Immune indicators: mouse serum IgG level (ELISA method); macrophage phagocytic activity (neutral red method).

[0032] Antioxidant indicators: serum SOD activity (xanthine oxidase method), MDA content (thiobarbituric acid method).

[0033] Metabolic indices: fasting blood glucose (glucose oxidase method), insulin level (radioimmunoassay) of rats, calculation of HOMA-IR index.

[0034] In vitro test example: In vitro test (antioxidant synergistic effect verification): Experimental materials: human hepatocyte cell line (HepG2), DPPH free radical, ABTS free radical, Fe²⁺-H2O2 system.

[0035] Test steps: Step A1, sample processing: dissolve the paste in PBS (pH 7.4), centrifuge and take the supernatant (sterilize by filtering with a 0.22 μm filter membrane).

[0036] Step A2, free radical scavenging experiment: DPPH method: Mix the sample with DPPH ethanol solution (0.1 mM), react for 30 min in the dark, and measure the absorbance at 517 nm.

[0037] ABTS method: ABTS free radical cation solution is mixed with the sample and the absorbance is measured at 734 nm.

[0038] Step A3, lipid peroxidation inhibition experiment: Hepatocytes were co-incubated with the Fe²⁺-H2O2 system, and the cellular MDA content was detected (thiobarbituric acid method).

[0039] The following is a data comparison table of animal experiment examples and in vitro test examples: Data comparison table: Animal experiments and in vitro tests have shown that the paste of the present invention has the following advantages: Immunomodulation: The serum IgG level of mice in the experimental group reached 12.5±1.2 mg / mL, an increase of 43.7% compared with the traditional paste formula (8.7±0.9 mg / mL).

[0040] Antioxidant synergistic effect: SOD activity was 152.3±10.5 U / mg prot, which was 44.2% higher than that of the single selenium group (105.6±8.7 U / mg prot).

[0041] Metabolic improvement: The HOMA-IR index of prediabetic rats decreased from 4.3±0.5 to 3.1±0.4 (P<0.01).

[0042] Application examples: The present invention also discloses the use of a Chinese medicine paste recipe rich in organic selenium and small molecule short peptides as in any one of the above embodiments 1-3 in any one of conditioning of sub-health status, prevention and treatment of chronic diseases, and postoperative or postpartum rehabilitation support.

[0043] Application in sub-health condition conditioning: Aiming at the common problems of low immunity, chronic fatigue syndrome, metabolic disorders, etc. in modern people, multi-target regulation is achieved through the "traditional Chinese medicine-selenium-peptide" ternary synergistic system. Experimental data show that the paste can increase the IgG level of mice by 40%, increase the SOD activity by 50%, and significantly improve sub-health related indicators.

[0044] Application in the prevention and treatment of chronic diseases: Prediabetes intervention: Delaying the progression of diabetes by improving insulin sensitivity (HOMA-IR index reduced by 29%).

[0045] Management of metabolic syndrome: Oligofructose regulates intestinal flora, synergizes with organic selenium and peptides to improve lipid metabolism, and reduces lipid peroxidation (MDA is reduced by 35%).

[0046] Treatment of gout in the non-acute phase: Similar prescriptions (such as Xiezhuo Tongbi Paste) have been clinically shown to lower uric acid to the standard range (mean 312 μmol / L) and reduce the recurrence of joint swelling and pain.

[0047] Application in postoperative / postpartum recovery support: Tonic ingredients such as astragalus and angelica are used to promote the recovery of Qi and blood, and microencapsulated small molecule peptides accelerate cell repair. It is suitable for scenarios such as Qi and blood deficiency after tumor surgery and postpartum.

[0048] This Chinese medicine ointment formula integrates traditional and modern technologies to achieve full-scenario application from chronic disease prevention to health management. Its core advantage lies in the triple innovation of "component-process-dosage form", such as phased extraction technology to improve the retention rate of active ingredients (selenium ≥ 95%, peptide ≥ 90%), and microencapsulation to solve the stability problem of peptides. In the future, it can be combined with transdermal drug delivery, intelligent customization and other directions to further expand the application boundaries.

[0049] The implementation principle of the present invention is as follows: the present invention discloses a Chinese medicine paste rich in organic selenium and small molecule short peptides, and its preparation method and application. It is a composite paste that integrates active ingredients of Chinese medicine, organic selenium and small molecule peptides. Among them, organic selenium (such as selenocystine) can activate glutathione peroxidase (GSH-Px) and enhance antioxidant capacity; small molecule peptides (molecular weight <1000Da) have the effect of promoting cell repair and regulating immunity. The activity of medicinal materials is maximized by staged extraction technology, and the peptide microencapsulation and selenium low-temperature addition process are combined to achieve a selenium retention rate of ≥95% and a peptide activity retention rate of ≥90%. The paste can synergistically improve immune, antioxidant and metabolic functions by activating the TLR4 / NF-κB pathway, enhancing GSH-Px activity and promoting IL-2 secretion, and is suitable for conditioning sub-health states such as prediabetes and chronic fatigue syndrome. The core of the present invention is to construct a ternary synergistic system of "Chinese medicine-selenium-peptide" and achieve efficient retention of active ingredients through innovative processes.

[0050] The embodiments of this specific implementation method are all preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Therefore, any equivalent changes made based on the structure, shape, and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides, characterized in that: The following components are included by mass percentage: Chinese herbal medicine extract: 50-70%; Organic selenium: 0.05-0.2%; Small molecule peptides: 5-15%; Auxiliary materials: 10-20%.

2. A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides according to claim 1, characterized in that: The Chinese medicine extract comprises a preparation made from the following raw materials in parts by weight: 30 g of astragalus, 25 g of tuckahoe, 15 g of angelica and 10 g of wolfberry.

3. A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides according to claim 1, characterized in that: The organic selenium is selected from one of selenium-enriched yeast and selenocystine.

4. A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides according to claim 1, characterized in that: The small molecule peptide is selected from collagen peptide or soybean peptide.

5. A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides according to claim 4, characterized in that: The molecular weight of the small molecule peptide is less than 1000Da.

6. A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides according to claim 1, characterized in that: The auxiliary material is oligofructose.

7. A Chinese medicinal paste prescription rich in organic selenium and small molecule short peptides according to claim 1, characterized in that: The small molecule peptide is processed by sodium alginate-chitosan microencapsulation, and the encapsulation rate is ≥85%.

8. A method for preparing a Chinese medicinal paste rich in organic selenium and small molecule short peptides as claimed in any one of claims 1 to 7, characterized in that: The following steps are involved: Step 1: Mixing, adding water to the Chinese herbal medicine extract for decoction, and extracting water-soluble polysaccharides and saponins after decoction twice; Step 2, enzymatic hydrolysis, adding 0.5-1.5% w / w cellulase to the decocted medicinal residue, enzymatic hydrolysis at 45° C. for 2 hours to release fat-soluble components, and then concentrating to form a concentrate; Step 3: Microencapsulation of the peptide, using sodium alginate-chitosan composite wall material, spray drying, air inlet temperature 160°C, air outlet temperature 80°C, to prepare a small molecule peptide, with an encapsulation rate ≥ 85%, and then adding the small molecule peptide to the concentrate, stirring and mixing at 60°C; Step 4: adding selenium at low temperature, concentrating in a vacuum low-temperature environment, adding organic selenium and auxiliary materials to the concentrate for homogenization to avoid oxidative inactivation of selenium; Step 5, freeze drying, pre-freeze to -40°C, sublimation drying for 24 hours, moisture ≤5%, to obtain a Chinese medicine paste recipe.

9. The method for preparing a Chinese medicinal paste rich in organic selenium and small molecule short peptides according to claim 8, characterized in that: In step 4, the vacuum low-temperature environment is: pressure -0.08MPa, temperature 50-55°C, and concentrated to a relative density of 1.20-1.

30.

10. Use of a traditional Chinese medicine paste rich in organic selenium and small molecule short peptides as described in any one of claims 1 to 7 in any one of conditioning of sub-healthy state, prevention and treatment of chronic diseases, and postoperative or postpartum rehabilitation support.

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