Hydrophilic gel and preparation method thereof

Abstract

The invention relates to the field of medical cosmetic materials, and discloses a hydrophilic gel and a preparation method thereof, and the hydrophilic gel comprises the following components in percentage by weight: 0.5%-1.0% of carbomer, 1%-2% of polyvinyl alcohol, 0.1%-0.5% of hydroxy propyl cellulose, 0.1%-0.5% of sodium hyaluronate, 3%-5% of glycerol, 0.05%-0.1% of EDTA-2Na and the balance of purified water. The hydrophilic gel prepared by a cold-phase preparation method has the characteristics of simplicity and convenience in operation, low energy consumption, heat-sensitive component protection, excellent mechanical property and multifunctional application, and is suitable for adhesion, isolation, hemostasis and healing promotion of tiny wounds.

Description

Technical Field

[0001] The present invention relates to the technical field of medical cosmetic materials, and in particular to a hydrophilic gel and a preparation method thereof. Background Art

[0002] Hydrophilic gels have been widely used in medicine, wound care and other fields due to their excellent biocompatibility and moisturizing properties. However, the traditional method for preparing hydrophilic gels usually involves a chemical cross-linking process under high temperature conditions, which not only has high energy consumption and complex operation, but also may cause the degradation of heat-sensitive components, thereby affecting the quality and performance of the gel. Specifically, the prior art has significant defects in the following aspects: First, the traditional method requires high temperature conditions, resulting in high energy consumption and increased production costs. Secondly, the complex chemical reaction steps increase the difficulty of operation and time cost, making the entire preparation process cumbersome and difficult to control. In addition, high temperature conditions can easily lead to the degradation of heat-sensitive components, which has a negative impact on the quality and performance of the final product. More importantly, the gels prepared by traditional methods perform poorly in terms of mechanical support, especially on tiny wounds where fluids often pass through and flush, and are prone to losing structural integrity and cannot effectively provide isolation, hemostasis and healing functions.

[0003] These defects limit the effectiveness and scope of hydrophilic gels in practical applications. For example, when treating tiny wounds, if the gel cannot provide sufficient mechanical support, it cannot effectively adhere to the wound surface, nor can it prevent external pollutants from entering the wound surface, thereby affecting the healing process. In addition, due to the destruction of heat-sensitive components by high temperature conditions, many active ingredients with potential therapeutic effects may not function in the final product, further reducing the practical application value of the gel. Summary of the invention

[0004] In view of the above-mentioned technical deficiencies, the technical problem to be solved by the present invention is to provide a hydrophilic gel and a preparation method thereof, aiming to solve the problems of high energy consumption, complex operation, easy degradation of heat-sensitive components and insufficient mechanical support existing in the prior art.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solution: The present invention provides a hydrophilic gel, comprising, by weight percentage, 0.5%-1.0% carbomer, 1%-2% polyvinyl alcohol, 0.1%-0.5% hydroxypropyl cellulose, 0.1%-0.5% sodium hyaluronate, 3%-5% glycerol, 0.05%-0.1% EDTA-2Na, and the balance is purified water.

[0006] A method for preparing a hydrophilic gel comprises the following steps: S1. Dissolve the polymer: dissolve carbomer, polyvinyl alcohol and sodium hyaluronate in purified water and stir evenly; S2, cross-linking reaction, adding an appropriate amount of triethanolamine, adjusting the pH to a set range, and forming a preliminary cross-linking network; S3, pouring the mixture into a mold and performing a freeze-thaw cycle to obtain a prepared gel; S4, post-treatment, soaking the prepared gel in deionized water for 24 hours to remove unreacted components.

[0007] Furthermore, in step S1, ultrasonic dispersion is performed for 30 minutes to ensure uniform distribution.

[0008] Furthermore, in step S2, the pH is adjusted to 4.5-6.5.

[0009] Furthermore, in step S3, the sample is frozen at -40°C for 12 hours, then thawed to room temperature for 24 hours, and the freeze-thaw cycle is repeated three times.

[0010] The beneficial effects of the present invention are as follows: the hydrophilic gel prepared by the cold phase preparation method of the present invention has the characteristics of simple operation, low energy consumption, protection of heat-sensitive components, excellent mechanical properties and multifunctional application, and is suitable for adhesion, isolation, hemostasis and promotion of healing of micro wounds. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0012] Figure 1 A schematic flow chart of a method for preparing a hydrophilic gel provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0013] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example

[0014] like Figure 1 As shown, this embodiment provides a method for preparing a hydrophilic gel, comprising the following steps: S1. Dissolve the polymers: dissolve carbomer, polyvinyl alcohol and sodium hyaluronate in purified water, stir well, and disperse by ultrasound for 30 minutes to ensure uniform distribution; S2, cross-linking reaction, adding an appropriate amount of triethanolamine, adjusting the pH to 4.5-6.5, and forming a preliminary cross-linking network; S3, pouring the mixture into a mold, freezing it at -40°C for 12 hours, and then thawing it to room temperature for 24 hours, repeating the freeze-thaw cycle three times to obtain the prepared gel; S4, post-treatment, soaking the prepared gel in deionized water for 24 hours to remove unreacted components.

[0015] This embodiment also provides a hydrophilic gel, comprising: in terms of weight percentage, 0.5%-1.0% carbomer in the gel as the main gel matrix; 1%-2% polyvinyl alcohol, which forms a microcrystalline structure through freeze-thaw cycles; 0.1%-0.5% hydroxypropyl cellulose, 0.1%-0.5% sodium hyaluronate, which provide moisture retention and biocompatibility; 3%-5% glycerin, a humectant, to prevent the gel from drying; 0.05%-0.1% EDTA-2Na, a chelating agent, to prevent metal ion interference; the remainder is purified water as a solvent.

[0016] The cold phase preparation of the hydrophilic gel used in this embodiment is a method of forming a gel network by physical or chemical crosslinking under low temperature conditions. The advantages of this method include simple operation, low energy consumption, and friendliness to heat-sensitive components, in order to obtain better mechanical support for the gel. Example

[0017] This embodiment is the second embodiment of the present invention. This embodiment is different from the first embodiment in that it provides an animal clinical experiment design of a hydrophilic gel and a preparation method thereof, evaluates the safety and effectiveness of the hydrophilic gel provided by the present invention in an animal model, and verifies the application effect of the gel in wound healing, hemostasis, isolation, etc.: The experimental rats were divided into three groups: experimental group, control group and blank group, with 8 rats in each group, for a total of 24 rats; the experimental group used hydrophilic gel to treat the wound; the control group used traditional dressings or other gels to treat the wound; the blank group did not receive any treatment and served as a negative control.

[0018] Furthermore, standardized wounds were prepared on the animals (select the outer edge of the sole of the rat's hind limb, make an incision from the heel to the toe, the incision length is generally about 1 cm, the depth is about 3-5 mm, avoid damaging the main blood vessels and nerves of the sole, and the operation is sterile). In the experimental group, the hydrophilic gel was evenly applied to the wound surface; in the control group, oil gauze was used; in the blank group, no treatment was performed.

[0019] The skin reactions, allergic symptoms and infection conditions of the animals were recorded. Wound tissue samples were collected on the 1st, 3rd, 7th and 14th days and stained with Masson staining to observe the changes in collagen fibers. At the same time, wound photos were taken to record the wound healing conditions. The changes in wound area of ​​the three groups were measured on the photos (% of the initial area). The healing rates of the three groups were calculated. Statistical software was used for data analysis to compare the variance differences between the experimental group and the control group. The chi-square test was used to evaluate the significance of each indicator. The results are shown in Tables 1 to 3.

[0020] Table 1: Comparison of wound area changes (% initial area, mean ± SD).

[0021] Time Experimental group (n=8) Control group (n=8) Blank group (n=8) Day 1 100.0±0.0 100.0±0.0 100.0±0.0 Day 3 78.2±4.5 86.1±5.3 95.3±4.1 Day 7 40.7±6.1 59.8±7.6 81.5±7.2 Day 14 11.6±3.2 28.9±5.8 50.4±8.6 Table 2: Table of skin reactions, allergic symptoms and infections. Table 3: Masson's stained collagen density (% area, mean ± SD).

[0022] Time Experimental group (n=8) Control group (n=8) Blank group (n=8) Day 3 19.3±3.5 12.8±2.7 8.1±1.9 Day 7 41.6±5.4 29.1±4.3 15.7±3.2 Day 14 64.8±6.7 46.5±5.6 25.3±4.8 According to Table 1, the healing rate was calculated to show that the healing rate of the experimental group on the 14th day was 88.4%, which was significantly higher than 71.1% (p<0.001) of the control group and 49.6% (p<0.001) of the blank group. The wounds treated with the hydrophilic gel of the present invention healed about 20% faster than those treated with traditional dressings and about 37% faster than those treated without treatment. On the 14th day, the wound surface areas of the experimental group and the control group were compared in pairs, with F=5.34 and p=0.032. The fluctuation of the healing rate data of the experimental group was significantly smaller than that of the control group, indicating that the treatment effect of the experimental group was more stable.

[0023] According to Table 2, combined with data analysis, the skin reactions and infection cases of the experimental group and the blank group were compared, and it was found that χ²=4.57, p=0.033, which shows that the hydrophilic gel of the present invention can significantly reduce the risk of skin reactions and infections compared with no treatment.

[0024] According to Table 3, combined with data analysis, it can be seen that the collagen density of the experimental group on the 14th day was 64.8%, which was significantly higher than that of the control group 46.5% and the blank group 25.3% (p<0.001), indicating that the hydrophilic gel of the present invention significantly accelerates tissue repair.

[0025] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A hydrophilic gel, characterized in that: In terms of weight percentage, the gel contains 0.5%-1.0% carbomer, 1%-2% polyvinyl alcohol, 0.1%-0.5% hydroxypropyl cellulose, 0.1%-0.5% sodium hyaluronate, 3%-5% glycerol, 0.05%-0.1% EDTA-2Na, and the remainder is purified water.

2. A method for preparing a hydrophilic gel, characterized in that: The following steps are involved: S1. Dissolve the polymer: dissolve carbomer, polyvinyl alcohol and sodium hyaluronate in purified water and stir evenly; S2, cross-linking reaction, adding an appropriate amount of triethanolamine, adjusting the pH to a set range, and forming a preliminary cross-linking network; S3, pouring the mixture into a mold and performing a freeze-thaw cycle to obtain a prepared gel; S4, post-treatment, soaking the prepared gel in deionized water for 24 hours to remove unreacted components.

3. The method for preparing the hydrophilic gel according to claim 2, characterized in that: In the step S1, ultrasonic dispersion is performed for 30 minutes to ensure uniform distribution.

4. The method for preparing the hydrophilic gel according to claim 2, characterized in that: In the step S2, the pH is adjusted to 4.5-6.

5.

5. The method for preparing the hydrophilic gel according to claim 2, characterized in that: In step S3, the sample is frozen at -40°C for 12 hours, then thawed to room temperature for 24 hours, and the freeze-thaw cycle is repeated three times.

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