Aquaculture water body purification filler and preparation process thereof

Abstract

The application provides a filler for purifying water bodies for aquaculture and a preparation process thereof, and the filler comprises aerobic filler A, anoxic filler B, autotrophic nitrifying bacteria, autotrophic denitrifying bacteria and water; the aerobic filler A comprises a granular mixture and volcanic rock particles; the granular mixture comprises diatomite, vermiculite powder, bentonite, activated white clay, wollastonite, potassium feldspar powder, dolomite powder, zeolite powder, manganese sandstone particles, quartz sand particles and a coagulant; and the anoxic filler B comprises calcium-magnesium-based diatomite, expanded vermiculite powder, calcium carbonate powder and elemental sulfur powder.The filler for purifying water bodies for aquaculture and the preparation process thereof can simultaneously remove ammonia nitrogen, nitrite and nitrate in the water body, and can provide a suitable habitat for autotrophic nitrifying bacteria and sulfur autotrophic denitrifying bacteria through the characteristics of the filler, so that ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in the water can be effectively removed without additional carbon sources.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of water purification, and particularly relates to a filler for aquaculture water purification and a preparation process thereof. BACKGROUND

[0002] The nitrogen in aquaculture water mainly comes from residual feed, metabolic products of cultured animals and aquatic plant and animal debris. According to research reports, 60% to 70% of the nitrogen in feed is excreted into water. The excessive content of nitrogen in aquaculture can cause the following hazards:

[0003] (1) Toxic effect of ammonia nitrogen. The increase of ammonia nitrogen in water can inhibit the excretion of fish white body ammonia, increase the concentration of ammonia in blood and tissue, reduce the blood oxygen carrying capacity, and increase the blood CO2 concentration. NH3 is not charged and has high lipid solubility, which can easily penetrate the cell membrane to directly cause fish poisoning, resulting in increased secretion and death of fish groups. NH3 can cause damage to fish gill epithelial cells and reduce the immunity of fish.

[0004] (2) Toxic effect of nitrite nitrogen. High concentration of NO2 can change the blood hemoglobin containing divalent iron (reduced hemoglobin) into high iron hemoglobin containing trivalent iron, thereby affecting the function of blood carrying oxygen, causing tissue hypoxia, and affecting the health of fish groups and even growth, thus creating conditions for the invasion of pathogens.

[0005] (3) Harm of nitrate nitrogen. Excessive content of nitrate nitrogen can lead to water eutrophication, affecting the health of fish and shrimp. Excessive nitrate nitrogen can form local short-range denitrification in the water environment, thereby increasing the concentration of nitrite and affecting the health of fish and shrimp. The main substances harmful to fish in water are nitrogen and nitrite. The water quality standard for aquaculture in China stipulates that the nitrogen is less than 0.5 mg / L and the nitrite is less than 0.2 mg / L.

[0006] Although the traditional chemical treatment method can effectively reduce the concentration of harmful substances in water, long-term use can destroy the ecological balance of water quality and cause potential harm to aquatic organisms. At the same time, the continuous input of chemical drugs also increases the operating cost. The biological method has low cost input, but the heterotrophic microorganisms need external carbon source, which is economically poor. At the same time, the external carbon source may cultivate pathogenic bacteria, which poses a threat to the survival of aquatic animals. Moreover, the traditional biological method can usually only solve one or two indicators of ammonia nitrogen, nitrite and nitrate nitrogen.

[0007] Therefore, how to remove ammonia nitrogen, nitrite and nitrate nitrogen in water without additional carbon source has become a technical problem to be solved in the present application. SUMMARY

[0008] The application aims to provide a filler for purifying aquaculture water and a preparation process thereof, and solve the technical problem of simultaneously removing ammonia nitrogen, nitrite and nitrate nitrogen in water without additional carbon source. The filler itself can provide a suitable habitat for autotrophic nitrifying bacteria and sulfur autotrophic denitrifying bacteria, effectively remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water, maintain clean water quality and ecological balance, and is suitable for water quality purification of aquaculture water.

[0009] The filler for purifying aquaculture water comprises aerobic filler A, anoxic filler B, autotrophic nitrifying bacteria, autotrophic denitrifying bacteria and water. The aerobic filler A comprises a particle mixture and volcanic rock particles. The particle mixture comprises diatomite, vermiculite powder, bentonite, activated white clay, wollastonite, potassium feldspar powder, dolomite powder, zeolite powder, manganese sandstone particles, quartz sand particles and a coagulant.

[0010] The anoxic filler B comprises calcium-magnesium-based diatomite, expanded vermiculite powder, calcium carbonate powder and elemental sulfur powder.

[0011] The mass ratio of the aerobic filler A to the anoxic filler B is (1-3):(1.5-3).

[0012] The content of each component in the particle mixture is 30-60g of diatomite, 30-60g of vermiculite powder, 20-40g of bentonite, 20-40g of activated white clay, 10-30g of wollastonite, 20-40g of potassium feldspar powder, 10-30g of dolomite powder, 10-30g of zeolite powder, 10-20g of manganese sandstone particles, 50-60g of quartz sand particles and 20-50g of foamed magnesite cement.

[0013] The mass ratio of the particle mixture to the volcanic rock particles is 1:0.5-3, and the content of autotrophic nitrifying bacteria is 1000ppm / 1kg of aerobic filler A, so that the content of sodium nitrate and sodium nitrite is between 80-200ppm. The particle size of the volcanic rock particles is 1.5-3cm.

[0014] The mass fraction of (diatomite+vermiculite powder) / bentonite is 0.5-3.

[0015] The mass fraction of (activated white clay+wollastonite) / bentonite is 0.5-3.

[0016] The mass fraction of (potassium feldspar powder+dolomite powder+zeolite powder+manganese sandstone particles+quartz sand particles) / bentonite is 0.5-10.

[0017] The mass fraction of foamed magnesite cement / particle mixture is 10%-20%.

[0018] The content of each component in the anoxic filler B is 40-50 g of calcium-magnesium-based diatomite, 40-50 g of expanded vermiculite powder, 50-100 g of calcium carbonate powder, and 50-100 g of elemental sulfur powder, by mass.

[0019] The content of the autotrophic denitrifying bacteria is 1000 ppm per 1 kg of the aerobic filler B, by mass, so that the content of sodium nitrate and sodium nitrite is between 80-200 ppm.

[0020] The mass ratio of (calcium-magnesium-based diatomite + expanded vermiculite powder + calcium carbonate powder) to elemental sulfur powder is 1-5.

[0021] A preparation process of a filler for purifying water bodies for aquaculture, specifically comprising the following steps:

[0022] Step S1: Mix each component in the granular mixture, add 10-30 g of water, and press into spherical particles with a particle size of 1.5-3 cm;

[0023] Step S2: Mix the spherical particles in step S1 with volcanic rock particles at a mass ratio of 1:0.5-3 to obtain the aerobic filler A;

[0024] Step S3: Submerge the aerobic filler A in step S2 in water, add 1000 ppm of autotrophic nitrifying bacteria per 1 kg of filler, maintain the content of sodium nitrate and sodium nitrite between 80-200 ppm, and form a biofilm on the surface of the filler;

[0025] Step S4: Place the material obtained in step S3 in a 0-4°C refrigerator for preservation to obtain the finished product;

[0026] Step S5: Mix each component in the anoxic filler B uniformly and place it in a sandwich pot to heat to 115-120°C until the elemental sulfur powder is fully dissolved and uniformly mixed with other components;

[0027] Step S6: Granulate the material obtained in step S5 to obtain particles with a particle size of 1-5 cm, and cool for standby use;

[0028] Step S7: Submerge the granular filler obtained in step S6 in water, add 1000 ppm of autotrophic denitrifying bacteria per 1 kg of filler, maintain the content of sodium nitrate and sodium nitrite between 80-200 ppm, and form a biofilm on the surface of the filler;

[0029] Step S8: Place the material in step S7 in a 0-4°C refrigerator for preservation to obtain the finished product.

[0030] Step S9: Finally, mix the finished product obtained in step S4 with the finished product obtained in step S8 at a mass ratio of (1-2):(1.5-3) to obtain the filler.

[0031] The mechanism of action of the biofilm autotrophic nitrifying bacteria in this solution is as follows:

[0032] Nitrosation reaction: NH4+ 1.5O2→ NO2 - + 2H + + H2O;

[0033] Nitrification reaction: NO2- + 0.5O2→ NO3 - ;

[0034] Overall reaction: NH4 + + 2O2→ NO3 - + 2H + + H2O;

[0035] The overall reaction equation for nitrification reaction and cell biomass production can also be written as:

[0036] Nitrosation reaction: 55NH4++ 5CO2+ 76O2→ C5H2NO2+ 54NO2-+ 52H2O + 109H + ;

[0037] Nitrification reaction: 400NO2 - + 5CO2+ NH4 + + 195O2+ 2H2O→ C5H7NO2+ 400NO3 - + H + ;

[0038] Overall reaction: NH4 + + 1.83O2+ 1.97HCO3 - → 0.0244C5H7NO2+ 0.976NO3 - + 2.90H2O

[0039] + 1.86CO2;

[0040] According to the above chemical formula, for every 1 g of ammonia converted to nitrate nitrogen, 4.1 g of dissolved oxygen and 7.05 g of alkalinity (1.69 g of inorganic carbon) are consumed, and 0.20 g of microbial biomass (0.105 g of organic carbon) and 5.85 g of CO2 (1.59 g of inorganic carbon) are produced.

[0041] The mechanism of action of the autotrophic denitrifying bacteria in this solution is as follows:

[0042] 10NO3 - + 11S+ 4.1HCO3 - + 0.5CO2+ 1.71NH4 + + 2.54H2O→ 0.92C5H7NO2+ 11SO 42- +

[0043] 5.4N2+962H + ;

[0044] According to the above chemical formula, every 1g of nitrate nitrogen is converted into nitrogen gas, which consumes 1.97g of alkalinity (calculated as carbonate) and 2.51g of sulfur, and produces N2.

[0045] Through the autotrophic nitrifying bacteria and autotrophic denitrifying bacteria in the filler of the application, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen can be removed in aerobic and anoxic environments, and finally converted into nitrogen gas.

[0046] The above chemical reaction formula provided in the present scheme is a basic principle introduction, and various filler components are added to provide trace elements, and the trace elements are made into fillers which can be released into the water body and continuously provide nutrient components for functional bacteria, wherein the diatomite, vermiculite powder and other components have the functions of the diatomite, vermiculite powder, bentonite, activated white clay, wollastonite, potassium feldspar powder, dolomite powder and zeolite powder, and the porous structure of the diatomite, vermiculite powder, bentonite, activated white clay, wollastonite, potassium feldspar powder, dolomite powder and zeolite powder is helpful for the attachment and colonization of microorganisms; calcium carbonate provides inorganic carbon source and balances ph; elemental sulfur powder provides sulfur source.

[0047] In the step S3, after the autotrophic nitrifying bacteria are added, the mixed solution is subjected to electric pulse treatment, the electric pulse intensity is 5-10V / cm, and the pulse width is 20-40 microseconds.

[0048] In the step S7, after the autotrophic denitrifying bacteria are added, the mixed solution is subjected to electric pulse treatment, the electric pulse intensity is 8-12V / cm, and the pulse intensity is 15-25 microseconds.

[0049] The present application achieves the following remarkable effects:

[0050] (1) The aerobic filler A and the anoxic filler B in the present scheme play a synergistic role, the aerobic filler A is mainly used for culturing autotrophic nitrifying bacteria, the bacteria need to utilize the nutrients in the filler and the water body under aerobic conditions to occur nitrification, which can convert ammonia nitrogen in the water body into nitrite, and then into nitrate, the anoxic filler B is mainly used for culturing autotrophic denitrifying bacteria, the bacteria need to utilize the nutrients in the filler and the water body under anoxic conditions to occur denitrification, which can convert nitrate and nitrite in the water body into nitrogen gas; through the two fillers, two conditions (aerobic and anoxic) are created to remove ammonia nitrogen, nitrite nitrogen and nitrate nitrogen;

[0051] (2) The natural diatomite has the characteristics of fine, loose, light, porous, strong water absorption and permeability, and can reach 19-65m 2 / g to provide more specific surface area, thereby increasing the amount of microorganisms attached and improving the autotrophic denitrification rate.

[0052] (3) The calcium-magnesium-based diatomite has strong cation exchange and water absorption and expansion properties, and can exchange ions with hydrogen ions generated in the process of sulfur autotrophy and autotrophic nitrification, thereby balancing the pH value; the water absorption and expansion property improves the hydrophilicity of the surface layer of the filter material, which is more conducive to the adhesion of microorganisms.

[0053] (4) The expanded vermiculite powder has high cation exchange capacity and cation adsorption capacity, can adsorb harmful substances in water, and the slow release of elements such as nitrogen, phosphorus, potassium, aluminum, iron, magnesium, and silicon contained therein can provide trace elements for the growth of health organisms; calcium carbonate and magnesium carbonate provide inorganic carbon sources for the growth of microorganisms, and can neutralize hydrogen ions generated in the processes of nitrification and denitrification; elemental sulfur powder provides an electron donor for sulfur autotrophic denitrification.

[0054] (5) The most important and innovative point of the present scheme, which is different from the prior art, is that the method for removing ammonia nitrogen and salt in the aquaculture water body is usually autotrophic nitrifying bacteria or autotrophic nitrifying bacteria + carrier method, and the present scheme creatively adopts the method of autotrophic nitrifying bacteria + autotrophic denitrifying bacteria, which can remove ammonia nitrogen, salt, and nitrate nitrogen.

[0055] (6) The present scheme increases the electric pulse treatment in the preparation process, and achieves the following technical effects:

[0056] First, the electric pulse treatment enhances the activity of microorganisms on the aerobic filler, promotes the growth and reproduction of the microorganisms, which helps to improve the efficiency of wastewater treatment;

[0057] Second, the electric pulse treatment helps to change the properties of the surface of the filler, improve the oxygen transmission rate, and thereby enhance the effect of aerobic reaction;

[0058] Third, the electric pulse treatment changes the microstructure of the filler, improves its strength and toughness;

[0059] Fourth, the electric pulse treatment accelerates the chemical reactions in the filler and improves its performance. BRIEF DESCRIPTION OF DRAWINGS

[0060] Figure 1 The purification effect curve diagram of the carrier prepared in Example 1 in a freshwater fish tank.

[0061] Figure 2 The purification effect curve diagram of the carrier prepared in Example 1 in a seawater fish tank.

[0062] Figure 3 The purification effect curve diagram of the carrier prepared in Example 1 in a South American prawn farm.

[0063] Figure 4The purification effect curve of the carrier prepared in Example 1 in a freshwater prawn farm.

[0064] Figure 5 The purification effect curve of the carrier prepared in Example 1 in a freshwater prawn farm. DETAILED DESCRIPTION

[0065] In order to more clearly illustrate the technical features of the present scheme, the present scheme will be described below through specific embodiments. Example 1

[0066] Reference Figure 1 The freshwater fish tank is filled with freshwater, and the breeding density is 2.5 kg / 100 L, and the water temperature is 25-28℃.

[0067] Step 1: Put the filler A (the filler includes: diatomite 30g, vermiculite powder 30g, bentonite 20g, activated white clay 20g, wollastonite 10g, potassium feldspar powder 20g, dolomite powder 10g, zeolite powder 10g, manganese sandstone particles 10g, quartz sand particles 50g, and condensation agent (foamed magnesite cement 50g) into the first two boxes of the 4-box filter of the aquarium fish tank, and install an aeration device at the bottom of the filter.

[0068] Step 2: Put the filler B (calcium-magnesium-based diatomite 40g, expanded vermiculite powder 40g, calcium carbonate powder 50g, and elemental sulfur powder 50g) into the last two boxes of the 4-box filter of the aquarium fish tank.

[0069] Step 3: Connect the configured aquarium fish tank filter to the water in the freshwater fish tank, and perform the cycle filtration.

[0070] At this time, for the filler A, the mass fraction of (diatomite + vermiculite powder) / bentonite = 3;

[0071] The mass fraction of (activated white clay + wollastonite) / bentonite = 1.5;

[0072] The mass fraction of (potassium feldspar powder + dolomite powder + zeolite powder + manganese sandstone particles + quartz sand particles) / bentonite = 5;

[0073] The total weight of the foamed magnesite cement / particle mixture = 19.2%.

[0074] For the filler B, the mass of (calcium-magnesium-based diatomite + expanded vermiculite powder + calcium carbonate powder) / elemental sulfur powder = 2.6.

[0075] The mass ratio of the aerobic filler A and the anoxic filler B is 1.44.

[0076]

[0077] As Figure 1As shown in Table 1, in the experimental group of Example 1 applying the filler, ammonia nitrogen can be removed by 96% within 1 week and maintained at a low level; nitrite nitrogen can be removed within 2 days and always stably maintained at about 0, which is much better than the blank group without applying the filler, nitrate nitrogen can be removed by more than 96% within 2 days and maintained at a low level, while the control group without applying the filler is in a state of continuously rising nitrate nitrogen.

[0078] The above verifies that the carrier for water purification provided by the present application can quickly start nitrification and denitrification reaction in a freshwater fish tank, saves application time by early biofilm formation, and has the performance of continuous denitrification, which has a significant contribution to solving the water quality problem of freshwater aquaculture. Example 2

[0079] The seawater fish tank is filled with seawater, and the breeding density is 2.5 kg / 100 L, and the water temperature is 25-28℃.

[0080] First step: Put the filler A (the filler includes: diatomite 60g, vermiculite powder 60g, bentonite 40g, activated white clay 40g, wollastonite 30g, potassium feldspar powder 40g, dolomite powder 30g, zeolite powder 30g, manganese sandstone particles 20g, quartz sand particles 60g, coagulant: foamed magnesite cement 50g) into the first two boxes of the 4-box filter of the aquarium fish tank, and install an aeration device at the bottom of the filter.

[0081] Second step: Put the filler B (calcium-magnesium-based diatomite 50g, expanded vermiculite powder 50g, calcium carbonate powder 100g, elemental sulfur powder 100g) into the last two boxes of the 4-box filter of the aquarium fish tank.

[0082] Third step: Connect the configured aquarium fish tank filter to the water in the seawater fish tank for circulating filtration.

[0083] At this time, for the filler A, the mass fraction of (diatomite + vermiculite powder) / bentonite is 3;

[0084] The mass fraction of (activated white clay + wollastonite) / bentonite is 1.75;

[0085] The mass fraction of (potassium feldspar powder + dolomite powder + zeolite powder + manganese sandstone particles + quartz sand particles) / bentonite is 4.5;

[0086] The total weight of foamed magnesite cement / particle mixture is 10.8%.

[0087] For the filler B, the mass of (calcium-magnesium-based diatomite + expanded vermiculite powder + calcium carbonate powder) / elemental sulfur powder is 2.

[0088] The mass ratio of the aerobic filler A to the anoxic filler B is 1.53.

[0089]

[0090] As Figure 2 As shown in Table 2, in the experimental group of Example 2 applying the filler, ammonia nitrogen can be removed by 94% within 1 week and maintained at a low level; nitrite nitrogen can be removed within 3 days and always stably maintained at about 0, which is much better than the blank group without applying the filler; nitrate nitrogen can be removed by more than 96% within 3 days and maintained at a low level, while the control group without applying the filler is in a state of continuously increasing nitrate nitrogen.

[0091] The above verifies that the carrier for water purification provided by the present application can quickly start nitrification and denitrification reaction in a seawater fish tank, saves application time by early biofilm formation, and has the performance of continuous denitrification, which has a significant contribution to solving the water quality problem of seawater aquaculture. Example 3

[0092] A South American prawn farm in Rizhao, Shandong, a cement aquaculture pond with a capacity of 100m 3 , salinity 1.5%-2%, stocking density: 0.5kg / 100L, water temperature 20-25℃. One round-shaped aeration barrel and one autotrophic denitrification filter barrel are made, and the bottoms of the two barrels are connected by pipes.

[0093] First step: Put the filler A (the filler includes: diatomite 120kg, vermiculite powder 120kg, bentonite 80kg, activated white clay 80kg, wollastonite 60kg, potassium feldspar powder 80kg, dolomite powder 60kg, zeolite powder 60kg, manganese sand particles 40kg, quartz sand particles 120kg, coagulant: foamed magnesite cement 100kg) into the aeration barrel and fill it with seawater, and aerate it with a fan.

[0094] Second step: Put the filler B (calcium-magnesium-based diatomite 100kg, expanded vermiculite powder 100kg, calcium carbonate powder 200kg, elemental sulfur powder 200kg) into the denitrification filter barrel and fill it with seawater.

[0095] Third step: Connect the configured filter system to the aquaculture pond for circulating filtration.

[0096] At this time, for filler A, the mass fraction of (diatomite + vermiculite powder) / bentonite = 3;

[0097] The mass fraction of (activated white clay + wollastonite) / bentonite = 1.75;

[0098] The mass fraction of (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand particles + quartz sand particles) / bentonite = 4.5;

[0099] The total weight of foamed magnesite cement / particle mixture = 10.8%.

[0100] For the filler B, the mass ratio of (calcium-magnesium-based diatomite + expanded vermiculite powder + calcium carbonate powder) / elemental sulfur powder is 2.

[0101] The mass ratio of the aerobic filler A to the anoxic filler B is 1.53.

[0102]

[0103] As Figure 3 As shown in Table 3, in the experimental group of Example 3 applying the filler, ammonia nitrogen can be removed by 90% within 1 week and maintained at a low level; nitrite nitrogen can be removed within 3 days and always stably maintained at about 0, which is far superior to the blank group without applying the filler; nitrate nitrogen can be removed by more than 96% within 3 days and maintained at a low level, while the control group without applying the filler is in a state of continuously rising nitrate nitrogen.

[0104] The above verifies that the carrier for water purification provided by the present application can quickly start nitrification and denitrification reaction in South American shrimp farms, save application time by early biofilm formation, and has the performance of continuous denitrification, which has a significant effect on solving the water quality problem of South American shrimp farms. Example 4

[0105] A grouper factory farm in Enshi, Hubei, a cement breeding pond with a pool capacity of 150m 3 , salinity 0.2%-0.6%, stocking density: 1.5kg / 100L, water temperature 20-25℃. One round-shaped aeration barrel and one autotrophic denitrification filter barrel are made, and the bottoms of the two barrels are connected by pipeline.

[0106] First step: Put the filler A (the filler includes: diatomite 120kg, vermiculite powder 120kg, bentonite 80kg, activated white clay 80kg, wollastonite 60kg, potassium feldspar powder 80kg, dolomite powder 60kg, zeolite powder 60kg, manganese sand particles 40kg, quartz sand particles 120kg, coagulant: foamed magnesite cement 100kg) into the aeration barrel and fill it with fresh water, and aerate it with a fan.

[0107] Second step: Put the filler B (calcium-magnesium-based diatomite 100kg, expanded vermiculite powder 100kg, calcium carbonate powder 200kg, elemental sulfur powder 200kg) into the denitrification filter barrel and fill it with seawater.

[0108] Third step: Connect the configured filter system to the breeding pond for circulating filtration.

[0109] At this time, for the filler A, the mass fraction of (diatomite + vermiculite powder) / bentonite is 3;

[0110] The mass fraction of (activated white clay + wollastonite) / bentonite is 1.75.

[0111] (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand particles + quartz sand particles) / bentonite = 4.5;

[0112] The total weight of the foamed magnesium oxychloride cement / granular mixture is 10.8%.

[0113] For filler B, by mass, (calcium-magnesium diatomaceous earth + expanded vermiculite powder + calcium carbonate powder) / elemental sulfur powder = 2.

[0114] The mass ratio of the aerobic packing material A to the anoxic packing material B is 1.53.

[0115]

[0116] like Figure 4 As shown in Table 4, in the experimental group using the packing material in Example 4, ammonia nitrogen was removed by 95% within one week and remained at a low level; nitrite nitrogen was removed within four days and remained stable at around 0, which was far better than the blank group without packing material. Nitrate nitrogen was removed by more than 98% within six days and remained at a low level, while nitrate nitrogen in the control group without packing material was constantly increasing.

[0117] The above verifies that the carrier for water purification provided by the present invention can quickly initiate nitrification and denitrification reactions in freshwater grouper farms. Because it saves application time by attaching the biofilm in advance and has continuous denitrification performance, it has a significant effect on solving the water quality problem in freshwater grouper farms. Example 5

[0118] A koi farm in Zhoukou, Henan Province, has cement breeding ponds with a volume of 150m³. 3 Salinity 0.05%-0.11%, stocking density: 2kg / 100L, water temperature 20-25℃. Make one round plant-shaped aeration tank and one autotrophic denitrification filter tank, and connect the bottoms of the two tanks through pipes.

[0119] Step 1: Put filler A (which includes: 120kg diatomaceous earth, 120kg vermiculite powder, 80kg bentonite, 80kg activated clay, 60kg wollastonite, 80kg potassium feldspar powder, 60kg dolomite powder, 60kg zeolite powder, 40kg manganese sand particles, 120kg quartz sand particles, and 100kg foamed magnesium oxychloride cement as coagulant) into the aeration tank, fill it with fresh water, and aerate it with a blower.

[0120] Step 2: Place packing material B (100kg calcium magnesium diatomaceous earth, 100kg expanded vermiculite powder, 200kg calcium carbonate powder, and 200kg elemental sulfur powder) into the denitrification filter tank and fill it with seawater.

[0121] Step 3: Connect the configured filtration system to the aquaculture pond for circulating filtration.

[0122] At this point, for filler A, by mass fraction, (diatomaceous earth + vermiculite powder) / bentonite = 3;

[0123] (Activated clay + wollastonite) / bentonite = 1.75;

[0124] (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand particles + quartz sand particles) / bentonite = 4.5;

[0125] The total weight of the foamed magnesium oxychloride cement / granular mixture is 10.8%.

[0126] For filler B, by mass, (calcium-magnesium diatomaceous earth + expanded vermiculite powder + calcium carbonate powder) / elemental sulfur powder = 2.

[0127] The mass ratio of the aerobic packing material A to the anoxic packing material B is 1.53.

[0128]

[0129] like Figure 5 As shown in Table 5, in the experimental group using the packing material in Example 5, ammonia nitrogen was removed by 95% within one week and maintained at a low level; nitrite nitrogen was removed completely within 6 days and remained stable at around 0, which was far superior to the blank group without packing material. Nitrate nitrogen was removed by more than 99% within 7 days and maintained at a low level, while the control group without packing material was inferior to the packing material group.

[0130] The above verifies that the carrier for water purification provided by the present invention can quickly initiate nitrification and denitrification reactions in koi farms. Because it saves application time by attaching the biofilm in advance and has continuous denitrification performance, it has a significant effect on solving the water quality problems in koi farms.

[0131] The above examples sufficiently demonstrate that the packing material provided in this solution can simultaneously remove ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen from water. Through its inherent properties, the packing material provides a suitable habitat for autotrophic nitrifying bacteria and sulfur-autotrophic denitrifying bacteria, effectively removing ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen from water without the need for an additional carbon source. Numerous combinations of components can be designed based on the above proportions to verify the results; these will not be detailed further in this solution.

[0132] It should be noted that the above-mentioned various embodiments all involve fillers A and fillers B, and the manufacturing methods thereof all include a biofilm formation process, and the biofilm formation is a bacteria cultivation process, and the successful biofilm formation means that the filler manufacturing is successful; the bacteria hung on the filler A are autotrophic nitrifying bacteria, and the bacteria hung on the filler B are autotrophic denitrifying bacteria, and the following embodiments all use the filler A (autotrophic nitrifying bacteria are hung thereon) or the filler B (autotrophic denitrifying bacteria are hung thereon) to carry out relevant experiments, and the relevant bacteria will not be described separately.

[0133] The technical features not described in the present application can be realized by or using the prior art, which will not be described here. Of course, the above description is not a limitation of the present application, and the present application is not limited to the above examples. Changes, modifications, additions or substitutions made by ordinary skilled persons in the technical field within the essential scope of the present application should also be within the protection scope of the present application.

Claims

1. A packing material for purifying aquatic aquaculture water, characterized in that, It includes aerobic packing material A, anoxic packing material B, autotrophic nitrifying bacteria, autotrophic denitrifying bacteria, and water. The aerobic packing material A includes a granular mixture and volcanic rock particles. The granular mixture includes diatomaceous earth, vermiculite powder, bentonite, activated clay, wollastonite, potassium feldspar powder, dolomite powder, zeolite powder, manganese sand particles, quartz sand particles, and a coagulant. The anoxic filler B includes calcium-magnesium diatomaceous earth, expanded vermiculite powder, calcium carbonate powder, and elemental sulfur powder; The mass ratio of the aerobic packing material A to the anoxic packing material B is (1-3):(1.5-3). By mass, the content of each component in the particulate mixture is as follows: diatomaceous earth 30-60g, vermiculite powder 30-60g, bentonite 20-40g, activated clay 20-40g, wollastonite 10-30g, potassium feldspar powder 20-40g, dolomite powder 10-30g, zeolite powder 10-30g, manganese sand particles 10-20g, quartz sand particles 50-60g, and the coagulant is foamed magnesium oxychloride cement 20-50g; The mass ratio of the particulate mixture to the volcanic rock particles is 1:0.5-3, and the content of autotrophic nitrifying bacteria is 1000ppm / 1kg aerobic packing A, so that the content of sodium nitrate and sodium nitrite is between 80-200ppm; the particle size of the volcanic rock particles is 1.5-3cm. By mass fraction, (diatomaceous earth + vermiculite powder) / bentonite = 0.5-3; (Activated clay + wollastonite) / bentonite = 0.5-3; (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand particles + quartz sand particles) / bentonite = 0.5-10; The total weight of the foamed magnesium oxychloride cement / granular mixture is 10%-20%.

2. The packing material for purifying aquatic water bodies according to claim 1, characterized in that, By mass, the contents of each component in the anoxic filler B are as follows: 40-50g of calcium magnesium-based diatomaceous earth, 40-50g of expanded vermiculite powder, 50-100g of calcium carbonate powder, and 50-100g of elemental sulfur powder.

3. The packing material for purifying aquatic water bodies according to claim 2, characterized in that, The content of the autotrophic denitrifying bacteria is 1000 ppm / 1 kg aerobic packing material B by mass, so that the content of sodium nitrate and sodium nitrite is between 80-200 ppm.

4. The packing material for purifying aquatic water bodies according to claim 3, characterized in that, By mass, (calcium-magnesium diatomaceous earth + expanded vermiculite powder + calcium carbonate powder) / elemental sulfur powder = 1-5.

5. A preparation process for a filler material for purifying aquatic aquaculture water, comprising the filler material for purifying aquatic aquaculture water as described in any one of claims 1-4, characterized in that, Specifically, the steps include the following: Step S1: Mix all the components in the granular mixture, add 10-30g of water, and press into spherical granules with a particle size of 1.5-3cm; Step S2: Mix the spherical particles from step S1 with volcanic rock particles at a mass ratio of 1:0.5-3 to form aerobic packing material A; Step S3: Immerse the aerobic packing material A from step S2 in water, add 1000 ppm of autotrophic nitrifying bacteria per 1 kg of packing material, and keep the content of sodium nitrate and sodium nitrite between 80-200 ppm until a biofilm forms on the surface of the packing material. Step S4: Place the material obtained in step S3 in a cold storage at 0-4℃ to obtain the finished product; Step S5: Mix all the components in the oxygen-deficient filler B evenly, and put it into a jacketed kettle and heat it to 115-120℃ until the elemental sulfur powder is fully dissolved and mixed evenly with the other components. Step S6: Granulate the material obtained in step S5 to form particles with a particle size of 1-5 cm, and cool them for later use. Step S7: Immerse the granular packing material obtained in step S6 in water, add 1000ppm of autotrophic denitrifying bacteria per 1kg of packing material, and keep the content of sodium nitrate and sodium nitrite between 80-200ppm until a biofilm forms on the surface of the packing material. Step S8: Place the materials from step S7 in a cold storage at 0-4℃ to obtain the finished product; Step S9: Finally, the finished product obtained in step S4 and the finished product obtained in step S8 are thoroughly mixed at a mass ratio of (1-3):(1.5-3) to obtain the filler.

6. The preparation process of a packing material for purifying aquatic water bodies according to claim 5, characterized in that, In step S3, after adding autotrophic nitrifying bacteria, the mixture is subjected to electrical pulse treatment with an electrical pulse intensity of 5-10V / cm and a pulse width of 20 to 40 microseconds.

7. The preparation process of a packing material for purifying aquatic water bodies according to claim 5, characterized in that, In step S7, after adding autotrophic denitrifying bacteria, the mixture is subjected to electrical pulse treatment with an electrical pulse intensity of 8-12V / cm and a pulse width of 15-25 microseconds.

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