A high-efficiency composting accelerating fungicide and its preparation method and application

Abstract

The present invention relates to a high-efficiency compost-promoting microbial agent, a preparation method, and an application thereof, belonging to the technical field of bio-agriculture and related industries. The microbial agent is composed of the following strains: Bacillus licheniformis DY-1, Bacillus massiliense MS, Bacillus aeruginosa MB, and Paenibacillus aeruginosa XWS-70; the viable cell count of each strain is greater than or equal to 5×10 8 CFU / g; the total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g. Its preparation method includes: strain activation, strain expansion culture, bacterial powder preparation, and strain compounding steps. Its application in rapid composting of organic waste has the following advantages: not only do the four strains not produce antagonism between each other, but they can also significantly accelerate cellulose degradation and improve the degree of composting.

Description

Technical Field

[0001] The invention belongs to the technical field of bio-agriculture and related industries, and particularly relates to a high-efficiency compost-promoting bacterium agent and a preparation method and application thereof. Background Art

[0002] The intensive production of agriculture and animal husbandry in my country has led to a large accumulation of agricultural organic waste. Composting is the primary method for effectively treating and utilizing organic waste. It can transform organic waste into organic fertilizer, decompose difficult-to-use high-molecular substances such as cellulose, and promote the formation of usable small molecules such as fulvic acid (FA) and humic acid (HA). Therefore, accelerating the degradation of cellulose into stable humus is a key issue in achieving high-quality compost.

[0003] Organic waste treatment methods primarily utilize physical, chemical, and biological technologies. Currently, biotechnology is often used to improve the quality of organic fertilizer production, by adding microbial agents to enhance compost efficiency and quality. Existing technologies primarily focus on single bacterial strains, such as those involved in cellulose degradation, while few composite agents address the challenges of efficient composting. Therefore, developing a composite microbial agent that can rapidly decompose agricultural organic waste and reduce resource waste is an effective approach to achieving high-quality production. Summary of the Invention

[0004] The first purpose of the present invention is to disclose a high-efficiency compost decay-promoting fungus agent.

[0005] The second purpose of the present invention is to disclose a preparation method of the above-mentioned high-efficiency decay-promoting fungus agent.

[0006] The third object of the present invention is to disclose the application of the above-mentioned high-efficiency decay-promoting fungi agent.

[0007] The purpose of the present invention is achieved through the following technical solutions:

[0008] A high-efficiency compost-promoting microbial agent, wherein: the microbial agent is composed of Bacillus licheniformis ( Bacillus lichen-like )CGMCC No.32099, Ureibacillus massiliensis CGMCC No.32598, Bacillus aeruginosa ( Bacillus aryabhattai ) CGMCC No.32597 and Paenibacillus odorifer CGMCC No. 33451 composition;

[0009] The mass ratio between the strains is one of A, B, C, D, E or F, where:

[0010] A is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus alteiraceus: Paenibacillus fragrant=1.5:3:2:1;

[0011] B is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus alteiraceus: Paenibacillus fragrant =1.5:1.5:2:1;

[0012] C is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus alteiraceus: Paenibacillus fragrant =1.5:1.5:3:1;

[0013] D is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus alteiraceus: Paenibacillus fragrant =1.5:2:3:1;

[0014] E is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus alteiraceus: Paenibacillus fragrant =1.5:2:1:1; or

[0015] F is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus alteiraceus: Paenibacillus fragrant =1.5:3:1:1;

[0016] The number of viable cells of each strain was ≥5×10 8 CFU / g; the total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g.

[0017] The method for preparing the high-efficiency compost-promoting bacterium agent described in the above technical solution comprises the following steps:

[0018] (1) Strain activation:

[0019] Bacillus licheniformis, Ureibacillus massiliensis , Bacillus arguta, Paenibacillus fragrant were inoculated into LB liquid medium and cultured to the logarithmic growth phase to obtain Bacillus licheniformis, Ureibacillus massiliensis , Bacillus arguta, Paenibacillus odorifer Seed liquid;

[0020] (2) Expansion culture of bacteria:

[0021] The Bacillus licheniformis obtained in step (1) Ureibacillus massiliensis , Bacillus arguta, Paenibacillus odorifer The seed solution was transferred to a new LB liquid medium at a 10% (v / v) inoculation rate, and the corresponding bacterial solution was obtained by expansion culture;

[0022] (3) Preparation of bacterial powder:

[0023] The Bacillus licheniformis obtained in step (2) Ureibacillus massiliensis , Bacillus arguta, Paenibacillus odorifer The bacterial suspension was centrifuged at 6000 rpm for 5 min at 4°C to collect the bacterial precipitate, which was freeze-dried to prepare bacterial powder of the four strains;

[0024] (4) Bacterial strain compounding:

[0025] The licheniformis bacteria obtained in step (3) are detected Ureibacillus massiliensis , Bacillus arguta, Paenibacillus odorifer The number of live bacteria contained in the bacterial powder is Ureabacillus from Marseille , Bacillus arguta, Paenibacillus odorifer By mixing according to the mass ratio described in claim 1, a high-efficiency compost-promoting fungus agent is obtained.

[0026] The preparation method described in the above technical solution, wherein: the culture conditions in steps (1) and (2) are:

[0027] Bacillus licheniformis DY-1 was cultured at 30°C and 180 rpm in a shaking incubator for 13–15 h;

[0028] Bacillus massiliense MS was cultured at 30°C and 180 rpm in a shaking incubator for 12–17 h;

[0029] Bacillus aeruginosa MB was cultured at 30°C and 180 rpm in a shaking incubator for 13–16 h;

[0030] Paenibacillus aerogenes XWS-70 was cultured at 30°C and shaken at 180 rpm for 12-15 hours.

[0031] The preparation method described in the above technical solution, wherein: the number of viable bacteria in the bacterial powder in step (4) is:

[0032] The number of viable cells of each strain was ≥5×10 8 CFU / g; the number of viable bacteria in the mixed agent is ≥10 9 CFU / g.

[0033] The application of the high-efficiency compost-promoting bacteria agent described in the above technical solution in the rapid decomposition and composting of organic waste.

[0034] Bacillus licheniformis ( Bacillus licheniformis ) CGMCC No.32099 (hereinafter referred to as: Bacillus licheniformis DY-1) was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies are light yellow, opaque, oval, slightly raised, and single or paired, rod-shaped, and have spores.

[0035] Ureibacillus massiliensis CGMCC No. 32598 (hereinafter referred to as Bacillus Marseille MS) was screened from mushroom residue and grown on beef extract peptone agar. The colonies were light yellow, translucent, round, slightly raised, smooth with neat edges, moist, rod-shaped, and had spores.

[0036] Bacillus arguta ( Bacillus aryabhattai ) CGMCC No.32597 (hereinafter referred to as: Bacillus arnoldii MB) was screened from mushroom residue and grew on beef extract peptone solid medium. The colonies were light yellow, opaque, round, slightly without protrusions, with a smooth surface and neat edges, moist, rod-shaped, and with spores.

[0037] Paenibacillus odorifer CGMCC No.33451 (hereinafter referred to as: Paenibacillus aerogenes XWS-70) was screened from mushroom residue and grown on beef extract peptone agar medium. The colonies were milky white, translucent, round, slightly raised, with a smooth surface and neat edges, moist, smooth texture, and spores.

[0038] The present invention has the following beneficial effects:

[0039] 1. The present invention screened and obtained a strain of Bacillus licheniformis DY-1, which has excellent β-glucosidase activity.

[0040] 2. The present invention screened and obtained a strain of Bacillus massiliense MS, which has excellent CMC enzyme activity.

[0041] 3. The present invention screened and obtained a strain of Bacillus agglomerans MB, which has excellent filter paper enzyme activity.

[0042] 4. The present invention screened and obtained a Paenibacillus aeruginosa XWS-70, which has excellent CMC enzyme activity.

[0043] 5. The present invention is to Bacillus licheniformis DY-1 ( Bacillus licheniformis DY-1), Bacillus massiliense MS ( Ureibacillus massiliensis MS), Bacillus arguta MB ( Bacillus aryabhattai MB), Paenibacillus aerogenes XWS-70 ( Paenibacillus odorifer XWS-70) were mixed and compounded, and it was found that not only did the four strains not produce antagonism among each other, but they could significantly accelerate the degradation of cellulose and form stable humus. After actual testing, the CMC enzyme activity, β-glucosidase activity and filter paper enzyme activity of the four strains after compounding reached 69.74±5.42 U / mL, 70.13±7.11 U / mL and 65.51±7.31 U / mL, respectively.

[0044] Strain deposit information:

[0045] 1. Bacillus licheniformis DY-1 of the present invention is classified and named Bacillus licheniformis Bacillus lichen-like It has been deposited in the General Microbiology Center of China Culture Collection Administration. The address of the depository is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC NO.32099 and the deposit date is September 29, 2024.

[0046] 2. The present invention's Bacillus massiliense MS is classified and named Ureibacillus massiliensis It has been deposited in the General Microbiology Center of China Culture Collection of Microorganisms (CGMCC). The address of the depository is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC NO.32598 and the deposit date is November 12, 2024.

[0047] 3. The present invention's Bacillus arguta MB is classified and named Bacillus arguta Bacillus aryabhattai It has been deposited in the General Microbiology Center of China Culture Collection Administration (CGMCC). The address of the depository is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC NO.32597 and the deposit date is November 12, 2024.

[0048] 4. The aerogenes bacillus XWS-70 of the present invention is classified and named

[0049] Paenibacillus odorifer

[0050] It has been deposited in the General Microbiology Center of China Culture Collection of Microorganisms (CGMCC). The address of the depository is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC NO.33451 and the deposit date is January 17, 2025. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 It is the process of strain screening and composite bacterial agent preparation;

[0052] Figure 2 is the evolutionary tree of strain DY-1;

[0053] Figure 3 is the evolutionary tree of strain MS;

[0054] Figure 4 is the evolutionary tree of strain MB;

[0055] Figure 5is the evolutionary tree of strain XWS-70;

[0056] Figure 6 These are the results of pairwise antagonism experiments between strains. DETAILED DESCRIPTION

[0057] To facilitate understanding of the technical solution of the present invention, the following further describes an efficient compost-promoting bacterium agent, a preparation method thereof, and applications of the present invention in conjunction with specific embodiments.

[0058] The process of strain screening and preparation of high-efficiency compost-promoting bacteria is shown in Figure 1 The specific preparation process is as described in the examples.

[0059] Example 1: Isolation of strains:

[0060] Take 10 g of mushroom residue and put it into 50 mL of sterilized water. Shake it at 30°C and 180 rpm for 30 minutes. Let it stand for 30 minutes. Take 1 mL of the supernatant and dilute it to 10 mL with sterilized water. -1 Continuously make 10-fold gradient dilutions. Pipette 0.5 mL of the suspension at different dilution multiples and spread it on the surface of beef extract peptone agar medium. Incubate at 30°C for 24-36 hours. Based on the morphology and color changes of the strains, select strains with different morphologies for streak culture on CMC-Na medium, β-glucoside medium, and filter paper strip medium. Select strains with good growth and store them at low temperatures.

[0061] The culture medium and its formula in the above embodiment are as follows:

[0062] Beef extract peptone agar medium: peptone: 10 g, beef extract: 5 g, NaCl: 5 g, agar: 20 g, distilled water: 1000 mL, pH: 7.2;

[0063] CMC-Na medium: CMC-Na: 15 g, MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, agar: 20 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0064] β-Glucoside medium: salicin: 5 g, MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, agar: 20 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0065] Filter paper strip culture medium: 30 filter paper strips (1×2 cm), MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, agar: 20 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0066] Example 2: Screening and identification of strains:

[0067] 1. Screening of strains:

[0068] The strains were screened by cellulase activity. The specific steps are as follows:

[0069] 1.1. Seed solution preparation:

[0070] Use a sterile inoculating loop to pick a single colony of the strain that grows well on the solid culture medium from the slant, inoculate it into LB liquid culture medium, place it at 30°C, shake it at 180 rpm, and culture it to the logarithmic growth phase to prepare the seed solution.

[0071] 1.2. Screening of cellulose-degrading bacteria:

[0072] The various sub-liquids were inoculated into CMC-Na liquid medium, β-glucosidase liquid medium and filter paper strip liquid medium at a 10% (v / v) inoculation rate, respectively, and cultured continuously at 30°C and 180 rpm for 7 days. The activities of CMCase, β-glucosidase and filter paper strip enzyme were determined by DNS method.

[0073] The culture medium and formula used in the above screening steps are as follows:

[0074] LB liquid medium: peptone: 10 g, yeast powder: 5 g, NaCl: 5 g, pH: 7.2;

[0075] CMC-Na liquid medium: CMC-Na: 5 g, MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0076] β-Glucoside liquid medium: salicin: 5 g, MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0077] Filter paper strip liquid culture medium: filter paper strips (1×2 cm): 30 strips, MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0078] According to the above screening steps, 4 strains with strong CMC enzyme activity (U / mL), β-glucosidase activity (U / mL) and filter paper enzyme activity (U / mL) were screened. The decay-promoting ability of each strain is shown in Table 1:

[0079] Table 1 Cellulase degradation activity of each strain

[0080]

[0081] 2. Identification of bacterial species:

[0082] 2.1 Physiological and biochemical tests:

[0083] 2.1.1. Inoculate the four strains obtained in step 1 onto beef extract peptone agar medium and culture at 30°C for 48 h. Observe and record the growth and morphology of the colonies. Observe and record the bacterial morphology under a microscope.

[0084] Bacillus licheniformis DY-1 was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, opaque, oval, slightly raised, and single or paired, rod-shaped, with spores.

[0085] Bacillus massiliense MS was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, translucent, round, slightly protruding, smooth and with neat edges, moist, rod-shaped, and had spores.

[0086] Bacillus arnoldii MB was screened from mushroom residue and grown on beef extract peptone solid culture medium. The colonies were light yellow, opaque, round, slightly without protrusions, with a smooth surface and neat edges, moist, rod-shaped, and with spores.

[0087] Paenibacillus aeruginosa XWS-70 was screened from mushroom residue and grown on beef extract peptone agar medium. The colonies were milky white, translucent, round, slightly convex, with a smooth surface and neat edges, moist, smooth texture, and spores.

[0088] 2.1.2. Perform physiological and biochemical tests on each strain using a multi-phase bacterial identification method. The specific procedures are as follows:

[0089] (1) Reagent strips used:

[0090] API 50CH reagent strips, API 20E reagent strips

[0091] (2) Supporting culture medium:

[0092] API 50 CHB / E medium, API 0.85% NaCl medium

[0093] (3) Preparation of bacterial suspension:

[0094] Use an inoculation tool to pick an appropriate amount of colonies from the plate and add them to API 50 CHB / E medium or API 0.85% NaCl medium, grind them carefully to make a uniform bacterial suspension.

[0095] (4) Prepare the training environment:

[0096] Prepare a culture box and pour about 5 mL of distilled water into the honeycomb cavity of the dish to create a wet chamber.

[0097] (5) Inoculation reagent strips:

[0098] Place the API 50CH or API 20E reagent strip in an incubation chamber and pipette the bacterial suspension into the vials of the strip. For the CIT, VP, and GEL vials in the API 20E reagent strip, ensure that the bacterial suspension fills the tube and cup. For the ADH, LDC, ODC, H2S, and URE vials, add mineral oil to create an anaerobic environment.

[0099] (6) Cultivation:

[0100] Place the inoculated reagent strips in an incubator and incubate at 36°C ± 2°C for 18-24 hours.

[0101] (7) Interpretation of results:

[0102] Observe the color changes of each tube on the test strip and refer to the test strip instructions to determine whether it is positive, weakly positive or negative.

[0103] The physiological and biochemical test results of the four strains obtained are shown in Table 2.

[0104] Table 2 Physiological and biochemical characteristics of strains

[0105]

[0106] Note: “+”: positive; “w”: weak positive; “-”: negative

[0107] 2.2 Molecular Validation

[0108] Each of the four strains was inoculated into LB liquid medium and incubated at 30°C in a shaker for 24 hours. The cells were then harvested by centrifugation at 12,000 rpm. DNA from strains DY-1, MS, MB, and XWS-70 was extracted using an Ezup column-based bacterial genomic DNA extraction kit. PCR amplification of strains DY-1, MS, MB, and XWS-70 was performed using primers 27F and 1492R; the nucleotide sequences of the primers are as follows:

[0109] 27F: 5'-AGAGTTTGATCCTGGCTCAG-3'

[0110] 1492R:5'-AGAGTTTGATCCTGGCTCAG-3'

[0111] After purification, the PCR product was sent to Shanghai Bioengineering Co., Ltd. for sequencing, and the sequencing results were compared with the sequences in the NCBI website database for homology analysis. Figure 2 The evolutionary tree of strain MS is shown in Figure 3 The evolutionary tree of strain MB is shown in Figure 4 The evolutionary tree of strain XWS-70 is shown in Figure 5 shown.

[0112] Finally, the physiological and biochemical characteristics of the four strains were combined with molecular verification to confirm that the four strains DY-1, MS, MB, and XWS-70 involved in the present invention were Bacillus licheniformis DY-1 ( Bacillus licheniformis DY-1), Bacillus massiliense MS ( Ureibacillus massiliensis MS), Bacillus arguta MB ( Bacillus aryabhattai MB), Paenibacillus aerogenes XWS-70 ( Paenibacillus odorifer XWS-70).

[0113] Example 3: Compound of high-efficiency decomposing bacteria agent:

[0114] 1. Antagonism experiment:

[0115] The 4 strains obtained were tested for antagonism between each other on solid culture medium using the filter paper method. The bacteria smeared on the plate were the base bacteria, and the filter paper smeared and placed on the base bacteria were the test bacteria. The growth of the base bacteria and the test bacteria was observed. If there is no inhibition zone between the two bacteria, it means there is no antagonism. Otherwise, it means there is antagonism between the strains. The results are as follows. Figure 6 As shown, there is no antagonism between these four strains.

[0116] 2. Compounding of strains:

[0117] The four decay-promoting strains were inoculated into LB liquid medium and cultured on a shaker until the logarithmic growth phase to obtain seed solutions of Bacillus licheniformis DY-1, Bacillus massiliense MS, Bacillus alteiren MB, and Paenibacillus aerogenes XWS-70 (the specific method was as follows: Bacillus licheniformis DY-1 was cultured on a shaker at 30°C and 180 rpm for 14 h; Bacillus massiliense MS was cultured on a shaker at 30°C and 180 rpm for 15 h; Bacillus alteiren MB was cultured on a shaker at 30°C and 180 rpm for 14 h; Paenibacillus aerogenes XWS-70 was cultured on a shaker at 30°C and 180 rpm for rpm shaking culture for 13 hours to obtain seed liquids of four strains); inoculation and expansion culture followed by freeze-drying (the specific method is as follows: the seed liquids of Bacillus licheniformis DY-1, Bacillus massiliense MS, Bacillus alteiraceus MB, and Paenibacillus aerogenes XWS-70 were transferred to new LB liquid medium at a 10% (v / v) inoculation rate, Bacillus licheniformis DY-1 was cultured at 30°C and 180 rpm shaking for 14 hours; Bacillus massiliense MS was cultured at 30°C and 180 rpm shaking for 15 hours; Bacillus alteiraceus MB was cultured at 30°C and 180 rpm shaking for 14 hours; Paenibacillus aerogenes XWS-70 was cultured at 30°C and 180 rpm shaking for 13 hours, and the corresponding bacterial liquids were expanded; the obtained four bacterial liquids were centrifuged at 6000 rpm at 4°C for 5 minutes to collect the bacterial precipitates, and freeze-dried to prepare bacterial powders of the four strains), and the bacterial powders of the four strains (viable cell count ≥ 5×10 8 CFU / g); the following mass ratios were used for compounding: (Bacillus licheniformis DY-1: Bacillus massiliense MS: Bacillus aeruginosa MB: Paenibacillus aerogenes XWS-70) = A: (1.5:3:2:1); B: (1.5:1.5:2:1); C: (1.5:1.5:3:1); D: (1.5:2:3:1); E: (1.5:2:1:1); F: (1.5:3:1:1). Six compound combinations were inoculated into CMC-Na medium, β-glucosidase medium, and filter paper strip medium, respectively, for determination of CMC enzyme activity, β-glucosidase activity, and filter paper enzyme activity. The results are shown in Table 3. The results in Table 3 show that the CMC enzyme activity of combination A is higher than that of other combinations, the β-glucosidase activity of combination B is higher than that of other combinations, and the filter paper enzyme activity of combination C is higher than that of other combinations; the CMC enzyme activity and filter paper enzyme activity of combination D are higher than those of other combinations, and the three enzyme activities of combinations E and F are relatively high, higher than the CMC enzyme activity, β-glucosidase activity and filter paper enzyme activity of the compound decay-promoting agent of CN118421517A.

[0118] Table 3 Cellulase activity of the composite bacterial agent

[0119]

[0120] The above description is only a preferred embodiment of the present invention and does not impose any formal or substantial limitation on the present invention. Any technician familiar with the present profession can make use of the technical content disclosed above without departing from the scope of the technical solution of the present invention, and any equivalent changes, modifications and evolutions made by them are equivalent embodiments of the present invention. At the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A high-efficiency compost-promoting microbial agent, characterized in that: The bacterial agent is composed of Bacillus licheniformis ( Bacillus licheniformis ) CGMCC No.32099, Bacillus Marseilles ( Ureibacillus massiliensis ) CGMCC No.32598, Bacillus aeruginosa ( Bacillus aryabhattai ) CGMCC No.32597 and Paenibacillus aerogenes ( Paenibacillus odorifer ) CGMCC No.33451; The mass ratio between the strains is one of A, B, C, D, E or F, where: A is the ratio of Bacillus licheniformis: Bacillus massiliense: Bacillus alteiraceus: Paenibacillus aerogenes = 1.5:3:2:1; B is the Bacillus licheniformis: the Bacillus massiliense: the Bacillus alteiraceus: the Paenibacillus aerogenes = 1.5:1.5:2:1; C is the ratio of Bacillus licheniformis: Bacillus massiliense: Bacillus alteiraceus: Paenibacillus aerogenes = 1.5:1.5:3:1; D is the ratio of Bacillus licheniformis: Bacillus massiliense: Bacillus alteiraceus: Paenibacillus aerogenes = 1.5:2:3:1; E is the Bacillus licheniformis: the Bacillus massiliense: the Bacillus alteiraceus: the Paenibacillus aerogenes = 1.5:2:1:1; or F is the ratio of Bacillus licheniformis: Bacillus massiliense: Bacillus alteiraceus: Paenibacillus aerogenes = 1.5:3:1:1; The number of viable cells of each strain was ≥5×10 8 CFU / g; the total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g.

2. The method for preparing the high-efficiency compost-promoting microbial agent according to claim 1, comprising the following steps: (1) Strain activation: Bacillus licheniformis ( Bacillus licheniformis ) CGMCC No.32099, Bacillus Marseilles ( Ureibacillus massiliensis ) CGMCC No.32598, Bacillus aeruginosa ( Bacillus aryabhattai ) CGMCC No.32597 and Paenibacillus aerogenes ( Paenibacillus odorifer ) CGMCC No. 33451 were inoculated into LB liquid culture medium and cultured to the logarithmic growth phase to obtain seed solutions of the Bacillus licheniformis, the Bacillus massiliense, the Bacillus aeruginosa, and the Paenibacillus aeruginosa; (2) Expansion culture of bacteria: The seed liquid of the Bacillus licheniformis, the Bacillus massiliense, the Bacillus argei, and the Paenibacillus aeruginosa obtained in step (1) is transferred to a new LB liquid culture medium at an inoculum volume of 10% v / v, and expanded and cultured to obtain the corresponding bacterial liquid; (3) Preparation of bacterial powder: The bacterial suspensions of the Bacillus licheniformis, the Bacillus massiliense, the Bacillus agglomerans, and the Paenibacillus aeruginosa obtained in step (2) were centrifuged at 6000 rpm for 5 minutes at 4°C to collect bacterial precipitates, which were freeze-dried to prepare bacterial powders of the four strains; (4) Bacterial strain compounding: The number of live bacteria contained in the bacterial powder of the Bacillus licheniformis, the Bacillus massiliense, the Bacillus argyi and the Paenibacillus aeruginosa obtained in the detection step (3) is detected, and the Bacillus licheniformis, the Bacillus massiliense, the Bacillus argyi and the Paenibacillus aeruginosa are mixed according to the mass ratio of claim 1 to obtain a high-efficiency compost-promoting bacterial agent.

3. The preparation method according to claim 2, wherein: The culture conditions for steps (1) and (2) are: The Bacillus licheniformis was cultured at 30° C. and 180 rpm in a shaking incubator for 13-15 hours; The Bacillus massiliense was cultured at 30° C. and 180 rpm in a shaking incubator for 12-17 hours; The Bacillus aeruginosa was cultured at 30° C. and 180 rpm in a shaking incubator for 13-16 hours; The Paenibacillus aerogenes was cultured at 30° C. and 180 rpm in a shaking incubator for 12-15 hours.

4. Use of the high-efficiency compost-promoting fungus agent according to claim 1 in the rapid decomposition and composting of organic waste.

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