Alcohol beverage containing multifunctional active ingredients and preparation method thereof

Abstract

The invention relates to the technical field of functional foods, in particular to an alcoholic beverage containing multifunctional active ingredients and a preparation method of the alcoholic beverage. The medicinal liquor comprises the following components in parts by weight: 2.25 g of ultramicro chondroitin, 0.05 to 0.1 part of a mesenchymal stem cell (MSCs) derivative, 0.02 to 0.05 part of immune globulin (IgG / IgA), 0.05 to 0.15 part of a growth factor, 0.05 to 0.15 part of targeted ligand B cells, 0.01 to 0.03 part of curcumin, 1 to 3 parts of temperature-sensitive and acid-sensitive double-response hydrogel, 0.1 to 0.3 part of a nanoscale protective layer material, 0.05 to 0.1 part of an antioxidant, 0.05 to 0.1 part of a preservative and 450 ml of alcohol base liquor, the temperature-sensitive-acid-sensitive double-response hydrogel is combined with a nano-scale protective layer targeted release mechanism system, and ultramicro chondroitin and high-quality bioactive components thereof are added into an alcohol product for the first time, so that the bioactive components of the ultramicro chondroitin can still keep the stability and functionality of the active components in the alcohol beverage.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and specifically to an alcoholic beverage containing multifunctional active ingredients and a preparation method thereof. Background Art

[0002] Functional alcoholic beverages are beverages that have specific functional ingredients added to traditional alcoholic beverages. These ingredients may include vitamins, minerals, herbal extracts or other substances that have health benefits. These beverages are usually designed to provide additional health benefits beyond the effects of alcohol, such as increased energy levels, improved sleep quality or enhanced immunity.

[0003] At present, the existing functional alcoholic beverages have poor stability of active ingredients, single functionality, and low survival rate of active ingredients in high alcohol environments, which need to be solved. Therefore, there is an urgent need for a functional alcoholic beverage that can maintain stability and high efficiency at different alcohol concentrations and a preparation method thereof. Summary of the invention

[0004] In view of the problems existing in the prior art, the purpose of the present invention is to provide an alcoholic beverage containing multifunctional active ingredients and a preparation method and application thereof. The present invention provides a method for adding super-microcarnitine and its high-quality bioactive ingredients to alcoholic products for the first time based on a temperature-sensitive and acid-sensitive dual-responsive hydrogel combined with a nano-level protective layer targeted release mechanism system, so that the bioactive ingredients can play a multifunctional application in alcoholic beverages; the application of super-microcarnitine and its high-quality bioactive ingredients in alcoholic beverages is an innovative technology. Super-microcarnitine has obvious advantages in stability, porous purification, and high efficiency in ingredient structure, production process, bioavailability, functional effect, and safety, and optimizes its taste, aroma, clarity, and shelf life.

[0005] To achieve the above object, the present invention provides the following technical solutions: An alcoholic beverage containing multifunctional active ingredients, comprising the following components in weight: 0.1-0.3 parts of super microcartilage, 0.05-0.1 parts of mesenchymal stem cell (MSCs) derivatives, 0.02-0.05 parts of immunoglobulins (IgG / IgA), 0.05-0.15 parts of growth factors, 0.05-0.15 parts of targeting ligand B cells, 0.01-0.03 parts of curcumin, 1-3 parts of temperature-sensitive and acid-sensitive dual-responsive hydrogels, 0.1-0.3 parts of nanoscale protective layer materials, 0.05-0.1 parts of antioxidants, 0.05-0.1 parts of preservatives, and 100-120 parts of alcohol base wine.

[0006] As a further preferred embodiment of the present invention, the mesenchymal stem cells (MSCs) derivatives are exosomes secreted by mesenchymal stem cells (MSCs); The growth factor is selected from epidermal growth factor EGF; The antioxidant is selected from at least one of vitamin C and vitamin E; The preservative is selected from at least one of potassium benzoate and potassium sorbate; The nano-scale protective layer material is selected from at least one of polylactic acid, polyethylene glycol, and hyaluronic acid; The alcohol content of the alcohol base wine is 8-53%.

[0007] A method for preparing an alcoholic beverage containing a multifunctional active ingredient, the specific method is as follows: 1) After fully mixing the super-microostigmin, mesenchymal stem cell (MSCs) derivatives, immunoglobulins (IgG / IgA) and growth factors according to weight proportions, the super-microostigmin, the mesenchymal stem cell (MSCs) derivatives, the immunoglobulins (IgG / IgA) and the growth factors are wrapped with a temperature-sensitive-acid-sensitive dual-responsive hydrogel, and the nano-scale protective layer material is used for secondary wrapping, and then the targeting ligand B cells are added to obtain a composite gel; 2) Add the composite gel to the alcohol-based wine, stir at 500-800r / min for 10-20min, then add curcumin, antioxidants and preservatives, filter the resulting mixture through a filter with a pore size of 0.20-0.22 μm, transfer the filtered liquid to a sterilizer, sterilize it with high-pressure steam at 121-123°C for 20-30min, cool it naturally to room temperature after sterilization, seal it in a light-proof condition, and store it at room temperature.

[0008] As a further preferred embodiment of the present invention, the temperature-sensitive and acid-sensitive dual-responsive hydrogel is prepared by using a temperature-sensitive material and an acid-sensitive material as raw materials, dissolving them in deionized water, adding a cross-linking agent, and initiating polymerization under ultraviolet light; The temperature-sensitive material is selected from poly(N-isopropylacrylamide); The acid-sensitive material is selected from polyacrylic acid; The cross-linking agent is selected from N, N, -methylenebisacrylamide; The ratio of the temperature-sensitive material, the acid-sensitive material, the deionized water and the cross-linking agent is (5-10) g: (5-10) g: (100-200) mL: (0.5-1.0) g; The specific preparation method is as follows: 1) Place (5-10) g of poly(N-isopropylacrylamide) and (5-10) g of polyacrylic acid in a container, add (100-200) mL of deionized water, and stir until completely dissolved. Then add (0.5-1.0) g of N,N,-methylenebisacrylamide to the above solution, and continue stirring until fully mixed to obtain a mixed solution; 2) Slowly pour the mixed solution into the mold, making sure the surface is flat and free of bubbles. Let it stand for 5-10 minutes to allow the solution to be evenly distributed. Place the mold under a 365nm UV lamp, 10-15cm away from the light source, turn on the UV lamp, and irradiate for 10-15 minutes until the solution is completely solidified into a transparent gel. 3) Take out the obtained transparent gel, rinse it repeatedly with deionized water for 3-5 times to remove the unreacted monomers and cross-linking agents, then soak it in deionized water and store it in a refrigerator at 3-5℃ for later use.

[0009] As a further preferred embodiment of the present invention, the preparation method of the composite gel is as follows: 1) Mix super-microostigmin, mesenchymal stem cell (MSCs) derivatives, immunoglobulin (IgG / IgA) and growth factors according to weight proportions to obtain a mixture, draw it with a syringe, and slowly add it into the temperature-sensitive-acid-sensitive dual-responsive hydrogel at a speed of 500-800 r / min while stirring. After the addition is completed, continue stirring for 10-20 minutes; 2) Add the nano-scale protective layer material to the above mixture, continue stirring for 5-10 minutes, and after mixing evenly, add the targeting ligand B cell red to the above mixture, continue stirring for 2-5 minutes, and then let it stand at room temperature for 30-50 minutes, then soak it in deionized water and store it in a refrigerator at 3-5°C.

[0010] Compared with the prior art, the present invention has the following beneficial effects: In the present invention, active ingredients such as super-soft droitin are released in a targeted manner, and the bioavailability is significantly improved by the targeted release in the oral-gastric dual stages. By incorporating high-quality active biological ingredients such as super-soft droitin and high-quality mesenchymal stem cell (MSCs) derivatives, alcoholic beverages are endowed with a variety of beneficial health effects such as strengthening bones, delaying aging, enhancing resistance, protecting the liver, and assisting in lowering blood sugar, etc., and the preparation process is mature, the processing materials are common, suitable for large-scale industrial production, and the cost is controllable; and an optimized packaging design is adopted, the shelf life of the product is maintained for 18 months when the seal is intact, low temperature and light-proof and dry, and 12 months when stored at room temperature. DETAILED DESCRIPTION

[0011] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention.

[0012] In the embodiment of the present invention, the mesenchymal stem cell (MSCs) derivative is an exosome secreted by the mesenchymal stem cells (MSCs); the growth factor is selected from epidermal growth factor EGF; the antioxidant is selected from vitamin C; the preservative is selected from potassium sorbate; the nanoscale protective layer material is selected from polylactic acid; and the alcohol content of the alcohol base wine is 53%. Example 1

[0013] An alcoholic beverage containing multifunctional active ingredients, characterized in that it comprises the following components in parts by weight: 0.1 parts of super-soft ostrich, 0.05 parts of exosomes secreted by mesenchymal stem cells (MSCs), 0.02 parts of immunoglobulin (IgG / IgA), 0.05 parts of epidermal growth factor EGF, 0.05 parts of targeting ligand B cells, 0.01 parts of curcumin, 1 part of temperature-sensitive and acid-sensitive dual-responsive hydrogel, 0.1 parts of polylactic acid, 0.05 parts of vitamin C, 0.05 parts of potassium sorbate, and 100 parts of alcohol base wine.

[0014] A method for preparing an alcoholic beverage containing a multifunctional active ingredient, the specific method is as follows: 1) Place 5g of poly(N-isopropylacrylamide) and 5g of polyacrylic acid in a container, add 100mL of deionized water, and stir until completely dissolved. Then add 0.5g of N,N,-methylenebisacrylamide to the above solution, and continue stirring until fully mixed to obtain a mixed solution; 2) Slowly pour the mixed solution into the mold, making sure the surface is flat and free of bubbles. Let it stand for 5 minutes to allow the solution to be evenly distributed. Place the mold under a 365nm UV lamp, 10 cm away from the light source, turn on the UV lamp, and irradiate for 10 minutes until the solution is completely solidified into a transparent gel. 3) Take out the obtained transparent gel, rinse it repeatedly with deionized water for 3 times to remove the unreacted monomer and cross-linking agent, then soak it in deionized water and store it in a refrigerator at 3°C ​​for later use, and a temperature-sensitive and acid-sensitive dual-responsive hydrogel can be obtained; 4) Mix ultra-microostigmin, exosomes secreted by mesenchymal stem cells (MSCs), immunoglobulin (IgG / IgA) and epidermal growth factor (EGF) according to weight proportions to obtain a mixture, draw it with a syringe, and slowly add it into the temperature-acid-sensitive dual-responsive hydrogel at a speed of 500 r / min while stirring. After the addition is completed, continue stirring for 10 minutes; 5) Add polylactic acid to the above mixture and continue stirring for 5 minutes. After the mixture is evenly mixed, add the targeting ligand B cell red to the above mixture and continue stirring for 2 minutes. Then, let it stand at room temperature for 30 minutes, soak it in deionized water, and store it in a refrigerator at 3°C ​​to obtain a composite gel. 6) Add the composite gel to the alcohol-based wine, stir at 500r / min for 10min, then add curcumin, vitamin C and potassium sorbate, filter the resulting mixture through a filter with a pore size of 0.20 μm, transfer the filtered liquid to a sterilizer, and sterilize it with high-pressure steam at 121°C for 20min. After sterilization, cool it naturally to room temperature, seal it in a light-proof condition, and store it at room temperature. Example 2

[0015] An alcoholic beverage containing multifunctional active ingredients, characterized in that it comprises the following components in parts by weight: 0.2 parts of super-soft ostrich, 0.08 parts of exosomes secreted by mesenchymal stem cells (MSCs), 0.03 parts of immunoglobulin (IgG / IgA), 0.10 parts of epidermal growth factor EGF, 0.10 parts of targeting ligand B cells, 0.02 parts of curcumin, 2 parts of temperature-sensitive and acid-sensitive dual-responsive hydrogel, 0.2 parts of polylactic acid, 0.08 parts of vitamin C, 0.08 parts of potassium sorbate, and 110 parts of alcohol base wine.

[0016] A method for preparing an alcoholic beverage containing a multifunctional active ingredient, the specific method is as follows: 1) Place 8g of poly(N-isopropylacrylamide) and 8g of polyacrylic acid in a container, add 160mL of deionized water, and stir until completely dissolved. Then add 0.8g of N,N,-methylenebisacrylamide to the above solution, and continue stirring until fully mixed to obtain a mixed solution; 2) Slowly pour the mixed solution into the mold, making sure the surface is flat and free of bubbles. Let it stand for 7 minutes to evenly distribute the solution. Place the mold under a 365nm UV lamp, 12cm away from the light source, turn on the UV lamp, and irradiate for 13 minutes until the solution is completely solidified into a transparent gel. 3) Take out the obtained transparent gel, rinse it repeatedly with deionized water for 4 times to remove the unreacted monomer and cross-linking agent, then soak it in deionized water and store it in a refrigerator at 4°C for later use, and a temperature-sensitive and acid-sensitive dual-responsive hydrogel can be obtained; 4) Mix ultra-microostigmin, exosomes secreted by mesenchymal stem cells (MSCs), immunoglobulin (IgG / IgA) and epidermal growth factor (EGF) according to weight proportions to obtain a mixture, draw it with a syringe, and slowly add it into the temperature-acid-sensitive dual-responsive hydrogel at a speed of 700 r / min while stirring. After the addition is completed, continue stirring for 15 minutes; 5) Add polylactic acid to the above mixture and continue stirring for 7 minutes. After the mixture is evenly mixed, add the targeting ligand B cell red to the above mixture and continue stirring for 3 minutes. Then, let it stand at room temperature for 40 minutes, soak it in deionized water, and store it in a refrigerator at 4°C to obtain a composite gel. 6) Add the composite gel to the alcohol-based wine, stir at 700r / min for 15min, then add curcumin, vitamin C and potassium sorbate, filter the resulting mixture through a filter with a pore size of 0.22 μm, transfer the filtered liquid to a sterilizer, sterilize it with high-pressure steam at 122°C for 25min, cool it naturally to room temperature after sterilization, seal it in a light-proof condition, and store it at room temperature. Example 3

[0017] An alcoholic beverage containing multifunctional active ingredients, characterized in that it comprises the following components in parts by weight: 0.3 parts of super-soft ostrich, 0.1 parts of exosomes secreted by mesenchymal stem cells (MSCs), 0.05 parts of immunoglobulin (IgG / IgA), 0.15 parts of epidermal growth factor EGF, 0.15 parts of targeting ligand B cells, 0.03 parts of curcumin, 3 parts of temperature-sensitive and acid-sensitive dual-responsive hydrogel, 0.3 parts of polylactic acid, 0.1 parts of vitamin C, 0.1 parts of potassium sorbate, and 120 parts of alcohol base wine.

[0018] A method for preparing an alcoholic beverage containing a multifunctional active ingredient, the specific method is as follows: 1) Place 10g of poly(N-isopropylacrylamide) and 10g of polyacrylic acid in a container, add 200mL of deionized water, stir until completely dissolved, then add 1g of N,N,-methylenebisacrylamide to the above solution, continue stirring until fully mixed, and obtain a mixed solution; 2) Slowly pour the mixed solution into the mold, making sure the surface is flat and free of bubbles. Let it stand for 10 minutes to evenly distribute the solution. Place the mold under a 365nm UV lamp, 15 cm away from the light source, turn on the UV lamp, and irradiate for 15 minutes until the solution is completely solidified into a transparent gel. 3) Take out the obtained transparent gel, rinse it repeatedly with deionized water for 5 times to remove the unreacted monomer and cross-linking agent, then soak it in deionized water and store it in a refrigerator at 5°C for later use, and a temperature-sensitive and acid-sensitive dual-responsive hydrogel can be obtained; 4) Mix ultra-microostigmin, exosomes secreted by mesenchymal stem cells (MSCs), immunoglobulin (IgG / IgA) and epidermal growth factor (EGF) according to weight proportions to obtain a mixture, draw it with a syringe, and slowly add it into the temperature-acid-sensitive dual-responsive hydrogel at a speed of 800 r / min while stirring. After the addition is completed, continue stirring for 20 minutes; 5) Add polylactic acid to the above mixture and continue stirring for 10 minutes. After the mixture is evenly mixed, add the targeting ligand B cell red to the above mixture and continue stirring for 5 minutes. Then, let it stand at room temperature for 50 minutes, soak it in deionized water, and store it in a refrigerator at 5°C to obtain a composite gel. 6) Add the composite gel to the alcohol-based wine, stir at 800r / min for 20min, then add curcumin, vitamin C and potassium sorbate, filter the resulting mixture through a filter with a pore size of 0.22 μm, transfer the filtered liquid to a sterilizer, sterilize it with high-pressure steam at 123°C for 30min, cool it naturally to room temperature after sterilization, seal it in a light-proof condition, and store it at room temperature.

[0019] Test experiment: Verification of the multifunctional health benefits of functional alcoholic beverages Objective: To verify the health effects of the functional alcoholic beverage of the present invention in strengthening bones, delaying aging, enhancing immunity, etc.; Materials Needed: Functional alcoholic beverage 1 L; Experimental animals (mice) 30; Cell culture medium (including skin cells) 50 mL; Human volunteers (healthy adults) 30.

[0020] Specific steps: 1. Bone strengthening effect verification (animal experiment): The experimental animals were randomly divided into three groups: a normal control group, a blank control group (ordinary liquor), and an experimental group (the beverage of Example 1); the normal control group, the blank control group, and the experimental group were fed with water, ordinary liquor, and the beverage of Example 1 (2 μL / g calculated based on body weight) every day for 30 days, and then the L-L5 lumbar vertebrae were taken, the surrounding muscles were removed, and the bone density was measured using a DPX-L dual-energy X-ray absorptiometry instrument (unit: g / cm2, instrument accuracy of 1%). The experimental results are shown in Table 1.

[0021] Table 1 Comparison of lumbar spine bone density in each group of mice (DEXA method, g / cm2) Group N / pcs <![CDATA[Lumbar bone density g / cm 2 > Normal control group 10 0.295±0.013 Blank control group (ordinary liquor) 10 0.297±0.016 Experimental group (drinks of Example 1) 10 0.329±0.021 It can be seen from Table 1 that the beverage of the present invention has a good bone strengthening effect and can effectively improve bone density.

[0022] 2. Verification of anti-aging efficacy (cell experiment): Skin cells were made into cell suspension, and the cell concentration was adjusted. 4 × 104 cells were inoculated in each well of a 24-well plate, and 800 μL was inoculated in each well. Three replicate wells were set up in each group. After 4 hours, the cells were divided into a normal control group, a hydrogen peroxide treatment group, a blank control group, and an experimental group. 100 μL of serum-free culture medium was added to the normal group and the hydrogen peroxide treatment group, ordinary liquor was added to the blank control group, and the beverage of Example 1 was added to the experimental group. The addition amount was 100 μmol / L. After culturing at 37°C for 72 hours, the cell antioxidant capacity (superoxide dismutase SOD activity) was calculated and detected. The experimental results are shown in Table 2.

[0023] Table 2 Content of superoxide dismutase (SOD) in each group (U / mg) Group Concentration μmol / L SOD (U / mg) Normal control group - 31.5±2.5 Hydrogen peroxide treatment group - 18.1±1.5 Blank control group 100 18.3±1.2 Experimental Group 2 100 25.3±2.7 It can be seen from Table 2 that the beverage of the present invention has the effect of protecting cells from oxidative damage and can effectively increase cell vitality.

[0024] 3. Verification of the efficacy of enhancing immunity (human clinical trial) Human volunteers were randomly divided into three groups: a normal control group, a blank control group (ordinary liquor), and an experimental group (the beverage of Example 1). The normal control group, the blank control group, and the experimental group were fed with water, ordinary liquor, and the beverage of Example 1 (2 μL / g calculated by body weight) every day for 30 days. Then, 15 mL of venous sterile blood was collected and coagulated with heparin to prepare plasma. The immunoglobulin IgG determination kit (ELISA, product number: M1245L96) was used to determine the content of immunoglobulin IgG in the plasma of each group using a fully automatic blood biochemical analyzer. The experimental results are shown in Table 3.

[0025] Table 3 Immunoglobulin IgG content in each group g / L Group Immunoglobulin IgG content g / L Normal control group 8.6 Blank control group 8.7 Experimental Group 17.63 It can be seen from Table 3 that the beverage of the present invention can significantly enhance the immune function of cells in the human body.

[0026] In summary, the functional alcoholic beverage of the present invention has significant effects in strengthening bones, delaying aging, enhancing immunity, etc.

[0027] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. An alcoholic beverage containing a multifunctional active ingredient, characterized in that: The invention comprises the following components in parts by weight: 0.1-0.3 parts of super microostigmin, 0.05-0.1 parts of mesenchymal stem cell (MSCs) derivatives, 0.02-0.05 parts of immunoglobulins (IgG / IgA), 0.05-0.15 parts of growth factors, 0.05-0.15 parts of targeting ligand B cells, 0.01-0.03 parts of curcumin, 1-3 parts of temperature-sensitive and acid-sensitive dual-responsive hydrogels, 0.1-0.3 parts of nanoscale protective layer materials, 0.05-0.1 parts of antioxidants, 0.05-0.1 parts of preservatives, and 100-120 parts of alcohol-based liquor.

2. An alcoholic beverage containing a multifunctional active ingredient according to claim 1, characterized in that: The mesenchymal stem cells (MSCs) derivatives are exosomes secreted by mesenchymal stem cells (MSCs); The growth factor is selected from epidermal growth factor EGF; The antioxidant is selected from at least one of vitamin C and vitamin E; The preservative is selected from at least one of potassium benzoate and potassium sorbate; The nano-scale protective layer material is selected from at least one of polylactic acid, polyethylene glycol, and hyaluronic acid; The alcohol content of the alcohol base wine is 8-53%.

3. A method for preparing an alcoholic beverage containing a multifunctional active ingredient according to claim 1, characterized in that: The specific method is as follows: 1) After fully mixing the super-microostigmin, mesenchymal stem cell (MSCs) derivatives, immunoglobulins (IgG / IgA) and growth factors according to weight proportions, the super-microostigmin, the mesenchymal stem cell (MSCs) derivatives, the immunoglobulins (IgG / IgA) and the growth factors are wrapped with a thermosensitive-acid-sensitive dual-responsive hydrogel, and the nanoscale protective layer material is used for secondary wrapping, and then the targeting ligand B cells are added to obtain a composite gel; 2) Add the composite gel to the alcohol-based wine, stir at 500-800 r / min for 10-20 min, then add curcumin, antioxidants and preservatives, filter the resulting mixed solution through a filter with a pore size of 0.20-0.22 μm, transfer the filtered liquid to a sterilizer, sterilize it with high-pressure steam at 121-123° C. for 20-30 min, cool it naturally to room temperature after sterilization, seal it in a light-proof condition, and store it at room temperature.

4. The method for preparing an alcoholic beverage containing a multifunctional active ingredient according to claim 3, characterized in that: The temperature-sensitive and acid-sensitive dual-responsive hydrogel is prepared by using a temperature-sensitive material and an acid-sensitive material as raw materials, dissolving them in deionized water, adding a crosslinking agent, and initiating polymerization under ultraviolet light.

5. The method for preparing an alcoholic beverage containing a multifunctional active ingredient according to claim 4, characterized in that: The temperature-sensitive material is selected from poly(N-isopropylacrylamide); The acid-sensitive material is selected from polyacrylic acid; The cross-linking agent is selected from N, N ， -Methylenebisacrylamide; The ratio of the temperature-sensitive material, the acid-sensitive material, the deionized water and the cross-linking agent is (5-10) g: (5-10) g: (100-200) mL: (0.5-1.0) g.

6. The method for preparing an alcoholic beverage containing a multifunctional active ingredient according to claim 3, characterized in that: The composite gel, its preparation method is specifically as follows: 1) Mix super-microostigmin, mesenchymal stem cell (MSCs) derivatives, immunoglobulin (IgG / IgA) and growth factors according to weight proportions to obtain a mixture, draw it with a syringe, and slowly add it into the temperature-sensitive-acid-sensitive dual-responsive hydrogel at a speed of 500-800 r / min while stirring. After the addition is completed, continue stirring for 10-20 minutes; 2) Add the nano-scale protective layer material to the above mixture and continue stirring for 5-10 minutes. After the mixture is evenly mixed, add the targeting ligand B cell red to the above mixture and continue stirring for 2-5 minutes. Then, let it stand at room temperature for 30-50 minutes, soak it in deionized water, and store it in a refrigerator at 3-5°C.