Full-automatic circulating tumor cell enrichment device

Abstract

The invention discloses a full-automatic circulating tumor cell enrichment device, and belongs to the technical field of circulating tumor cell enrichment. A concave frame, a positioning rack, a movable frame, a positioning gear, a fixed clamping block, a movable connecting rod and a jacking spring are arranged, a limiting plate extrudes the jacking spring to deform, and the movable frame is pushed to move on one side of the concave frame; a positioning gear rolls on the surface of a positioning rack until a movable frame moves to a proper height on one side of a concave frame, a movable connecting rod is loosened, a limiting plate is jacked up in a mounting groove under the springback effect of a jacking spring, and the movable connecting rod drives a fixed clamping block to move upwards in a rectangular sliding groove to be clamped on the surface of the positioning gear; a fixing nut is screwed down to clamp and fix a movable connecting rod, so that a fixed clamping block is clamped and fixed on the surface of a positioning gear and cannot be randomly separated, a movable frame is clamped and fixed at a proper height on the surface of a concave frame, and a fixed clamp can be clamped and fixed at the top ends of test tubes with different lengths.

Description

Technical Field

[0001] The invention relates to the technical field of circulating tumor cell enrichment, and in particular to a fully automatic circulating tumor cell enrichment device. Background Art

[0002] Circulating tumor cells refer to tumor cells that have shed from primary tumors or metastatic tumors and entered the peripheral blood circulation. As a general term for various types of tumor cells present in the peripheral blood, they are shed from solid tumor lesions (primary lesions, metastatic lesions) spontaneously or during diagnostic and treatment operations. Most circulating tumor cells undergo apoptosis or are phagocytosed after entering the peripheral blood. A few are able to escape and anchor and develop into metastatic lesions, increasing the risk of death in patients with malignant tumors. During tumor treatment, regular testing of the number and characteristic changes of circulating tumor cells in the blood can provide real-time information on the tumor's response to treatment, thereby adjusting the treatment plan in a timely manner. When cells are tested, it is not convenient to detect circulating tumor cells due to their extremely low content in the blood. In order to improve the sensitivity of circulating tumor cell detection, discover trace amounts of tumor cells in the early stages of the tumor, and achieve early screening and diagnosis, cell enrichment devices are often used to enrich circulating tumor cells. Circulating tumor cells enriched by the cell enrichment device have a complete cell morphology, and contain information such as the genome, transcriptome, and proteome of the tumor cells. By analyzing the enriched circulating tumor cells, more comprehensive tumor characteristics can be obtained, providing more basis for tumor diagnosis.

[0003] When the existing fully automatic circulating tumor cell enrichment device clamps and fixes the test tube containing circulating tumor cells, since the test tubes have different lengths, the fixed clamp is stuck on the surface of the test tube during clamping and fixing. It is difficult to arbitrarily adjust the position of the fixed clamp according to the length of the test tube so that the fixed clamp is stuck in the central position of the test tube surface. When the test tube is operated inside the cell enrichment device, the test tube is prone to tilt, causing the circulating tumor cells inside the test tube to shake, affecting the cell enrichment device from enriching the circulating tumor cells inside the test tube. At the same time, a cleaning liquid storage box and a waste liquid storage box are provided on the surface of the cell enrichment device. The cleaning liquid storage box and the waste liquid storage box are generally placed on the same desktop with the cell enrichment device. When the cell enrichment device is moved on the desktop, the cleaning liquid storage box and the waste liquid storage box are prone to tip over on the desktop under the pull of the catheter. Therefore, improvement is needed.

[0004] Based on this, the present invention designs a fully automatic circulating tumor cell enrichment device to solve the above problems. Summary of the invention

[0005] The object of the present invention is to provide a fully automatic circulating tumor cell enrichment device to solve the problem that the existing fully automatic circulating tumor cell enrichment device proposed in the above background technology is that when clamping and fixing a test tube containing circulating tumor cells, the test tubes have different lengths, and the fixed clamp is clamped on the surface of the test tube during clamping and fixing. It is difficult to arbitrarily adjust the position of the fixed clamp according to the length of the test tube so that the fixed clamp is clamped at the central position of the test tube surface. When the test tube is operated inside the cell enrichment device, the test tube is prone to tilt, causing the circulating tumor cells inside the test tube to shake, affecting the cell enrichment device to enrich the circulating tumor cells inside the test tube. At the same time, a cleaning liquid storage box and a waste liquid storage box are provided on the surface of the cell enrichment device. The cleaning liquid storage box and the waste liquid storage box are generally placed on the same desktop with the cell enrichment device. When the cell enrichment device is moved on the desktop, the cleaning liquid storage box and the waste liquid storage box are prone to tipping over on the desktop under the pull of the catheter.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A fully automatic circulating tumor cell enrichment device comprises a cell enrichment instrument body, a placement rack is symmetrically and rotatably installed on one side of the cell enrichment instrument body, a cleaning liquid storage box and a waste liquid storage box are respectively arranged inside two groups of the placement racks, and the cleaning liquid storage box and the waste liquid storage box are connected with the cell enrichment instrument body through a conduit, the cell enrichment instrument body is located at a central position between the two groups of placement racks and is inlaid with a fixed structure, a concave rack is fixedly installed inside the cell enrichment instrument body, a mobile rack is slidably installed on one side of the concave rack, a clamping block is fixedly installed at the bottom of the mobile rack, and two groups of test tubes are respectively clamped on both sides of the mobile rack.

[0008] As a further scheme of the present invention, a placement groove is symmetrically provided on one side of the cell enrichment instrument body, arc-shaped limit grooves are symmetrically provided on the bottom of both sides of the inner wall of the placement groove, a protective door panel is symmetrically rotatably installed on one side of the cell enrichment instrument body away from the placement groove, first rubber pads are fixedly installed on the top and bottom of both sides of the cell enrichment instrument body, and the positions of the two groups of first rubber pads correspond to each other, and second rubber pads are fixedly installed at the central position of one side of the top and inner bottom of the cell enrichment instrument body, and the first rubber pad and the second rubber pad correspond to the positions of the protective door panel.

[0009] As a further solution of the present invention, first limiting protrusions are fixedly installed on both sides of the placement rack, the first limiting protrusions correspond to the positions of the arc-shaped limiting grooves, and the first limiting protrusions are slidably connected to the inside of the arc-shaped limiting grooves.

[0010] As a further solution of the present invention, the fixed structure includes a circular mounting block, an arc-shaped slide groove is symmetrically provided on one side of the circular mounting block, an arc-shaped slide rod is fixedly installed inside the arc-shaped slide groove, a fixed clamping plate is rotatably installed at the central position of the surface of the circular mounting block between the two groups of arc-shaped slide grooves, a hand-holding ring is fixedly installed at the central position of the fixed clamping plate away from the side of the circular mounting block, a sliding block is symmetrically fixedly installed on the side of the fixed clamping plate close to the circular mounting block, and the position of the sliding block corresponds to the arc-shaped slide groove, and the sliding block is slidably connected to the surface of the arc-shaped slide rod, and the surface of the arc-shaped slide rod on one side of the sliding block is provided with an arc-shaped reset spring.

[0011] As a further solution of the present invention, T-shaped slide grooves are symmetrically provided on both sides of the concave frame, a positioning rack is fixedly installed at the central position inside the concave frame, an elastic pad is fixedly installed on one side of the bottom of the concave frame, and two supporting slots are respectively provided on both sides of the top of the elastic pad, and the supporting slots correspond to the positions of the test tubes.

[0012] As a further solution of the present invention, the movable frame includes two groups of fixed clips, and the fixed clips correspond to the positions of the supporting slots, a T-shaped limit slider is fixedly installed on one side of the fixed clip, the T-shaped limit slider corresponds to the position of the T-shaped slide slot, and the T-shaped limit slider is slidably connected to the inside of the T-shaped slide slot, a mounting frame is fixedly installed between the two groups of fixed clips, a positioning gear is rotatably installed inside the mounting frame, and the surface of the positioning gear is meshed with the positioning rack.

[0013] As a further solution of the present invention, an installation groove is provided inside the clamping block, a sliding groove is connected to the bottom of the installation groove, a rectangular slide groove is connected to the top of the installation groove, limiting slide grooves are symmetrically provided on both sides of the inner wall of the rectangular slide groove, a fixed card block is slidably installed inside the rectangular slide groove, and second limiting protrusions are symmetrically fixedly installed on both sides of the fixed card block, the second limiting protrusion corresponds to the position of the limiting slide groove, and the second limiting protrusion is slidably connected to the inside of the limiting slide groove.

[0014] As a further solution of the present invention, a movable connecting rod is fixedly installed on the bottom of the fixed block, and the bottom end of the movable connecting rod extends to the sliding slot through the mounting groove, and a limiting plate is fixedly installed on the surface of the movable connecting rod at the top of the mounting groove, and a lifting spring is provided on the surface of the movable connecting rod located inside the mounting groove, and one end of the movable connecting rod located inside the sliding groove passes through the sliding groove and is fixedly installed with a fixed stud, and a fixing nut is provided on the surface of the fixed stud.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention is provided with a concave frame, a positioning rack, a moving frame, a positioning gear, a fixed card block, a moving connecting rod and a jacking spring, and the fixing nut is loosened to press the moving connecting rod downward, so that the moving connecting rod drives the fixed card block to move down inside the rectangular slide groove and disengage from the positioning gear. At the same time, the moving connecting rod drives the limit plate to move down inside the installation groove to squeeze the jacking spring to deform, and pushes the moving frame to move on one side of the concave frame, so that the positioning gear rolls on the surface of the positioning rack until the moving frame moves to a suitable height on one side of the concave frame. The moving connecting rod is loosened and the limit plate is lifted up inside the installation groove under the rebound action of the jacking spring, so that the moving connecting rod drives the fixed card block to move up inside the rectangular slide groove and is clamped on the surface of the positioning gear, and the fixing nut is tightened to clamp and fix the moving connecting rod, ensuring that the fixed card block is clamped and fixed on the surface of the positioning gear and will not detach at will, thereby the moving frame is clamped and fixed at a suitable height on the surface of the concave frame, ensuring that the fixed clamp can be clamped and fixed on the top of test tubes of different lengths.

[0017] 2. The present invention provides a placement groove, a cleaning liquid storage box, a waste liquid storage box, a placement rack, an arc-shaped return spring and a fixed card plate. The hand-held ring is rotated to drive the fixed card plate to rotate a certain angle on one side of the circular mounting block to detach from the placement rack. At the same time, the fixed card plate drives the sliding block to slide on the surface of the arc-shaped slide rod when rotating, squeezing the arc-shaped return spring to deform, and rotating the placement rack to make the placement rack tilt inside the placement groove. The cleaning liquid storage box and the waste liquid storage box are respectively placed in the corresponding placement racks, and then the placement rack is lifted up and inserted into the placement groove. The cleaning liquid storage box and the waste liquid storage box are placed in the corresponding placement grooves, and the hand-held ring is released to rotate on the arc-shaped return spring. The sliding block is lifted up and slides on the surface of the arc-shaped sliding rod under the rebound effect, so that the sliding block is reset inside the arc-shaped sliding groove. At the same time, the sliding block drives the fixed clamping plate to rotate on the surface of the circular mounting block and clamp on one side of the two sets of placement racks, so as to facilitate the placement rack to be clamped and fixed inside the placement groove, to ensure that the placement rack is clamped inside the placement groove and will not rotate at will, to ensure that the cleaning liquid storage box or the waste liquid storage box is clamped inside the placement groove and can be moved together with the cell enrichment instrument body, to avoid the cleaning liquid storage box or the waste liquid storage box being dumped on the desktop under the pull of the catheter when the cell enrichment instrument body is moved, causing the cleaning liquid storage box or the waste liquid storage box to leak liquid inside the card. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0019] Figure 1 It is a cross-sectional structural schematic diagram of the present invention;

[0020] Figure 2It is a schematic diagram of the expanded structure of the present invention;

[0021] Figure 3 It is a schematic diagram of the expanded cross-sectional structure of the main body and the placement rack of the cell enrichment instrument of the present invention;

[0022] Figure 4 For the present invention Figure 3 A schematic diagram of the enlarged structure at A in the middle;

[0023] Figure 5 It is a schematic diagram of the structure of the circular mounting block and the arc-shaped sliding rod of the present invention;

[0024] Figure 6 It is a cross-sectional structural schematic diagram of the circular mounting block, the arc-shaped return spring and the fixed clamping plate of the present invention;

[0025] Figure 7 It is a cross-sectional structural schematic diagram of the concave frame, the movable frame and the clamping block of the present invention;

[0026] Figure 8 It is a schematic cross-sectional view of the positioning gear, the clamping block and the lifting spring of the present invention.

[0027] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0028] 1. Cell enrichment instrument body; 101. Placement slot; 102. Arc-shaped limit slot; 103. Protective door panel; 104. First rubber pad; 105. Second rubber pad; 2. Placement rack; 201. First limit convex block; 3. Cleaning liquid storage box; 4. Waste liquid storage box; 5. Fixed structure; 501. Round mounting block; 502. Arc-shaped slide; 503. Arc-shaped slide bar; 504. Fixed card plate; 505. Hand-held ring; 506. Sliding block; 507. Arc-shaped return spring; 6. Concave rack; 601. T-shaped slide; 602, positioning rack; 603, elastic pad; 604, supporting slot; 7, moving frame; 701, fixed clamp; 702, T-type limit slider; 703, mounting frame; 704, positioning gear; 8, clamping block; 801, mounting slot; 802, sliding slot; 803, rectangular slide slot; 804, limit slide slot; 805, fixed block; 806, second limit protrusion; 807, moving connecting rod; 808, limit plate; 809, lifting spring; 810, fixing stud; 811, fixing nut; 9, test tube. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] See also Figure 1-Figure 4 , the present invention provides a technical solution:

[0031] A fully automatic circulating tumor cell enrichment device comprises a cell enrichment instrument body 1, a placement rack 2 is symmetrically and rotatably mounted on one side of the cell enrichment instrument body 1, two groups of placement racks 2 are respectively provided with a cleaning liquid storage box 3 and a waste liquid storage box 4, and the cleaning liquid storage box 3 and the waste liquid storage box 4 are connected to the cell enrichment instrument body 1 through a catheter.

[0032] As a further solution of the present invention, a placement groove 101 is symmetrically provided on one side of the main body 1 of the cell enrichment instrument, and arc-shaped limiting grooves 102 are symmetrically provided on both sides of the inner wall of the placement groove 101, and first limiting protrusions 201 are fixedly installed on both sides of the placement rack 2, respectively, and the first limiting protrusions 201 correspond to the positions of the arc-shaped limiting grooves 102, and the first limiting protrusions 201 are slidably connected to the inside of the arc-shaped limiting grooves 102. The rotation of the placement rack 2 drives the first limiting protrusion 201 to slide inside the corresponding arc-shaped limiting grooves 102, thereby limiting the placement rack 2, ensuring that the placement rack 2 does not rotate excessively when rotating inside the placement groove 101, so that the placement rack 2 is tilted inside the placement groove 101, which facilitates the tilting of the cleaning liquid storage box 3 and the waste liquid storage box 4 into the placement rack 2, and avoids the cleaning liquid storage box 3 and the waste liquid storage box 4 from tilting too much when being inserted into the placement rack 2, resulting in leakage of the liquid inside the cleaning liquid storage box 3 and the waste liquid storage box 4.

[0033] A protective door plate 103 is symmetrically installed on one side of the cell enrichment instrument body 1 away from the placement groove 101, and first rubber pads 104 are fixedly installed on the top and bottom of both sides of the cell enrichment instrument body 1, and the positions of the two groups of first rubber pads 104 correspond to each other; second rubber pads 105 are fixedly installed at the central position of one side of the top and bottom of the cell enrichment instrument body 1, and the first rubber pad 104 and the second rubber pad 105 correspond to the positions of the protective door plate 103.

[0034] After the test tube 9 is clamped and fixed at a suitable position inside the cell enrichment instrument body 1, the protective door panel 103 is rotated and closed on the front side of the cell enrichment instrument body 1, so that the two sets of protective door panels 103 are pressed against the surface of the second rubber pad 105, and the second rubber pad 105 is squeezed to deform, thereby clamping and fixing the protective door panels 103 on the front side of the cell enrichment instrument body 1, ensuring that the protective door panels 103 will not open at will when the cell enrichment instrument body 1 is working.

[0035] When the test tube 9 needs to be taken out or adjusted, the protective door panel 103 is rotated open, and the protective door panel 103 is tightly attached to the two sides of the cell enrichment instrument body 1, so that the protective door panel 103 is stuck on the surface of the first rubber pad 104, squeezing the first rubber pad 104 to deform, thereby clamping and fixing the protective door panel 103 on both sides of the cell enrichment instrument body 1, ensuring that when the movable rack 7 inside the cell enrichment instrument body 1 is adjusted or the test tube 9 is clamped and fixed, the protective door panel 103 will not rotate at will and collide with the operator.

[0036] As a further embodiment of the present invention, Figure 5-6 The cell enrichment instrument body 1 shown is located at the central position between the two groups of placement racks 2 and is inlaid with a fixed structure 5, which includes a circular mounting block 501, and an arc-shaped slide groove 502 is symmetrically arranged on one side of the circular mounting block 501, and an arc-shaped slide rod 503 is fixedly installed inside the arc-shaped slide groove 502. A fixed card plate 504 is rotatably installed at the central position between the two groups of arc-shaped slide grooves 502 on the surface of the circular mounting block 501, and a hand-held ring 505 is fixedly installed at the central position of the fixed card plate 504 away from the circular mounting block 501; a sliding block 506 is symmetrically fixedly installed on the side of the fixed card plate 504 close to the circular mounting block 501, and the sliding block 506 corresponds to the position of the arc-shaped slide groove 502, and the sliding block 506 is slidably sleeved and connected to the surface of the arc-shaped slide rod 503, and the surface of the arc-shaped slide rod 503 on one side of the sliding block 506 is provided with an arc-shaped return spring 507, and the card plate 504 is in the shape of a long strip, and the two sides of the card plate 504 are respectively clamped in the corresponding placement rack 2 to clamp and fix the placement rack 2. After the hand-held ring 505 is rotated to drive the card plate 504 to rotate 90 degrees, the card plate 504 is separated from the placement rack 2, thereby releasing the placement rack 2.

[0037] The rotating hand-held ring 505 drives the fixed card plate 504 to rotate on the surface of the circular mounting block 501, so that the fixed card plate 504 drives the sliding block 506 to slide inside the corresponding arc-shaped slide groove 502, and the sliding block 506 slides on the surface of the arc-shaped slide bar 503 to squeeze the arc-shaped return spring 507 to deform, until the fixed card plate 504 rotates to a suitable angle on the surface of the circular mounting block 501 to detach from the placement rack 2. The placement rack 2 can be rotated freely inside the placement slot 101, and the placement rack 2 is rotated out of the placement slot 101, so that the cleaning liquid storage box 3 and the waste liquid storage box 4 can be inserted into the placement rack 2, and then the placement rack 2 is reversed and pushed to be inserted into the placement slot 101.

[0038] When it is necessary to clamp and fix the placement rack 2, the hand-held ring 505 is loosened and the sliding block 506 is lifted up to slide on the surface of the arc slide rod 503 under the rebound action of the arc return spring 507. The fixed clamping plate 504 is rotated on the surface of the circular mounting block 501 under the drive of the sliding block 506 until the fixed clamping plate 504 is lifted up and reset and clamped on the surface of the placement rack 2. Under the rebound action of the arc return spring 507, the sliding block 506 is lifted up and reset, so that the sliding block 506 drives the clamping plate 502 to rotate and reset from the vertical state to the horizontal state, so that the clamping plate 503 is clamped on the surface of the placement rack 2, thereby clamping and fixing the placement rack 2 inside the placement groove 101, ensuring that the placement rack 2 is stuck in the placement groove 101 and will not rotate out and tilt at will, so that the cleaning liquid storage box 3 and the waste liquid storage box 4 are fixed inside the placement groove 101 with the placement rack 2, and can be synchronously transported and moved with the cell enrichment instrument main body 1.

[0039] As a further embodiment of the present invention, Figure 7 As shown, a concave frame 6 is fixedly installed inside the main body 1 of the cell enrichment instrument, and T-shaped slide grooves 601 are symmetrically provided on both sides of the concave frame 6. A positioning rack 602 is fixedly installed at the central position inside the concave frame 6, and an elastic pad 603 is fixedly installed on one side of the bottom of the concave frame 6. Two supporting grooves 604 are respectively provided on both sides of the top of the elastic pad 603, and the supporting grooves 604 correspond to the positions of the test tubes 9.

[0040] A mobile rack 7 is slidably mounted on one side of the concave rack 6, and two groups of test tubes 9 are respectively clamped on both sides of the mobile rack 7. The mobile rack 7 includes two groups of fixed clamps 701, and the fixed clamps 701 correspond to the positions of the support slots 604; a T-shaped limit slider 702 is fixedly mounted on one side of the fixed clamp 701, and the T-shaped limit slider 702 corresponds to the position of the T-shaped slide slot 601, and the T-shaped limit slider 702 is slidably connected to the inside of the T-shaped slide slot 601. A mounting rack 703 is fixedly mounted between the two groups of fixed clamps 701, and a positioning gear 704 is rotatably mounted inside the mounting rack 703, and the surface of the positioning gear 704 is meshed with the positioning rack 602;

[0041] When the test tube 9 is stuck in the fixed clamp 701, the bottom end of the test tube 9 rests on the top of the supporting slot 604, so that the elastic pad 603 supports the bottom end of the test tube 9, to avoid that when the test tube 9 is stuck in the fixed clamp 701, the bottom end of the test tube 9 directly contacts with the cell enrichment instrument body 1, and the cell enrichment instrument body 1 causes wear to the bottom end of the test tube 9, and at the same time, to avoid that the test tube 9 is suspended and fixed in the fixed clamp 701, so that the fixed clamp 701 is subjected to excessive force, which affects the service life of the fixed clamp 701.

[0042] Pushing the fixed clamp 701 drives the T-shaped limiting slider 702 to slide inside the corresponding T-shaped slide groove 601, thereby limiting the up and down movement of the fixed clamp 701, ensuring that the fixed clamp 701 will not be tilted and stuck when moving up and down on the surface of the concave frame 6. At the same time, in the process of the fixed clamp 701 moving up and down, the positioning gear 704 rolls on the surface of the positioning rack 602. By clamping and fixing the positioning gear 704, the positioning gear 704 is clamped and meshed at different heights of the positioning rack 602, so that the fixed clamp 701 is clamped and fixed at different heights on the surface of the concave frame 6.

[0043] like Figure 8 As shown, a clamping block 8 is fixedly installed at the bottom of the movable frame 7, and an installation groove 801 is provided inside the clamping block 8. The bottom end of the installation groove 801 is connected to a sliding groove 802, and the top of the installation groove 801 is connected to a rectangular slide groove 803, and limiting slide grooves 804 are symmetrically provided on both sides of the inner wall of the rectangular slide groove 803; a fixed clamping block 805 is slidably installed inside the rectangular slide groove 803, and second limiting protrusions 806 are symmetrically fixedly installed on both sides of the fixed clamping block 805, the second limiting protrusion 806 corresponds to the position of the limiting slide groove 804, and the second limiting protrusion 806 is slidably connected to the inside of the limiting slide groove 804, and the limiting plate 808 slides inside the installation groove 801 and is clamped at the top of the installation groove 801, and cannot pass through the installation groove 801 to enter the inside of the rectangular slide groove 803, thereby blocking and limiting the limiting plate 808.

[0044] A movable link 807 is fixedly installed at the bottom of the fixed block 805, and the bottom end of the movable link 807 passes through the installation slot 801 and extends to the sliding slot 802. A limiting plate 808 is fixedly installed on the surface of the movable link 807 located at the top of the installation slot 801, and a lifting spring 809 is provided on the surface of the movable link 807 located inside the installation slot 801; one end of the movable link 807 located inside the sliding slot 802 passes through the sliding slot 802 and is fixedly installed with a fixed stud 810, and a fixing nut 811 is provided on the surface of the fixed stud 810.

[0045] When the movable connecting rod 807 is pushed to drive the fixed block 805 to slide inside the rectangular slide groove 803, the fixed block 805 drives the second limiting protrusion 806 to slide inside the corresponding limiting slide groove 804, thereby limiting the fixed block 805, ensuring that the fixed block 805 will not be tilted and stuck when sliding up and down inside the rectangular slide groove 803, affecting the clamping and fixing of the positioning gear 704 by the fixed block 805. At the same time, the fixed block 805 is clamped and fixed to the bottom of the positioning gear 704, ensuring that the positioning gear 704 will not rotate at will inside the mounting frame 703, and preventing the positioning gear 704 from rolling at will on the surface of the positioning rack 602.

[0046] Push the movable connecting rod 807 to drive the limit plate 808 to move downward inside the installation groove 801, squeeze the lifting spring 809 to deform, and at the same time drive the fixed block 805 to disengage from the positioning gear 704, until the movable frame 7 is moved to a suitable position on the surface of the concave frame 6, release the movable connecting rod 807, and under the rebound action of the lifting spring 809, the limit plate 808 is lifted inside the installation groove 801, so that the limit plate 808 drives the movable connecting rod 807 to move upward until the fixed block 805 is meshed and clamped on the surface of the positioning gear 704, tighten the fixing nut 811 to fit it tightly on the surface of the clamping block 8, thereby fixing the movable connecting rod 807 to ensure that the fixed block 805 is meshed and clamped on the surface of the positioning gear 704 and will not disengage at will.

[0047] During operation, after rotating the placement rack 2 to insert the cleaning liquid storage box 3 and the waste liquid storage box 4 into the placement rack 2, the placement rack 2 is reversed and the placement rack 2 is inserted into the placement groove 101 on the surface of the cell enrichment instrument main body 1. At the same time, the cleaning liquid storage box 3 and the waste liquid storage box 4 are connected to the cell enrichment instrument main body 1 through the conduit, and then the movable rack 7 is lifted up and slides up and down on the surface of the concave rack 6 until the movable rack 7 is moved to a suitable height on the surface of the concave rack 6, and the fixing nut 811 is tightened to make the fixed clamping block 805 inside the clamping block 8 clamp and fix the positioning gear 704, so that the positioning gear 704 is clamped and fixed at a suitable height on the surface of the positioning rack 602 to ensure that the movable rack 7 will not slide freely on the surface of the concave rack 6, and then the test tube 9 is clamped and fixed on one side of the fixed clamp 701, so that the bottom end of the test tube 9 is placed on the supporting clamping groove 604 on the top of the elastic pad 603, and the protective door panel 103 is closed to start the cell enrichment instrument main body 1 to enrich the cells in the test tube 9.

[0048] Working principle of the present invention:

[0049] The hand-held ring 505 is held and rotated to rotate the fixed card plate 504, so that the fixed card plate 504 drives the sliding block 506 to slide on the surface of the corresponding arc slide rod 503 to squeeze the arc return spring 507 to deform, until the fixed card plate 504 is rotated to a suitable angle to disengage from the placement rack 2, and the placement rack 2 is pulled to rotate the placement rack 2 out of the placement groove 101, so that the placement rack 2 drives the first limiting protrusion 201 to slide inside the corresponding arc limiting groove 102, until the placement rack 2 rotates out of the placement groove 101 and tilts at a certain angle, and the cleaning liquid storage box 3 and the waste liquid storage box 4 are inserted into the two sets of corresponding placement racks 2, and the cleaning liquid storage box 3 and the waste liquid storage box 4 are inserted into the two sets of corresponding placement racks 2 through the catheter. The waste liquid storage box 4 is connected to the main body 1 of the cell enrichment instrument, and the placement rack 2 is pushed to rotate into the placement groove 101. After driving the cleaning liquid storage box 3 and the waste liquid storage box 4 to be placed into the corresponding placement groove 101, the hand-held ring 505 is released, and the sliding block 506 is lifted up to slide on the surface of the arc slide bar 503 under the rebound effect of the arc return spring 507, so that the sliding block 506 drives the fixed card plate 504 to rotate on the surface of the circular mounting block 501 until the fixed card plate 504 rotates and is clamped on the surface of the placement rack 2, and the placement rack 2 is clamped and fixed in the placement groove 101, so that the cleaning liquid storage box 3 and the waste liquid storage box 4 are placed in the corresponding placement groove 101;

[0050] The protective door plate 103 is rotated to be close to both sides of the cell enrichment instrument body 1, so that the protective door plate 103 is stuck on the surface of the first rubber pad 104, the first rubber pad 104 is squeezed and deformed, and the protective door plate 103 is clamped and fixed on both sides of the cell enrichment instrument body 1, and the fixing nut 811 is loosened, and the fixing stud 810 is pressed to drive the moving connecting rod 807 to slide inside the installation groove 801, so that the moving connecting rod 807 drives the limiting plate 808 to move downward inside the installation groove 801 to squeeze the lifting spring 809 to deform, and at the same time, the moving connecting rod 807 drives the fixed card block 805 to move downward inside the rectangular slide groove 803 to disengage from the positioning gear 704, and push the fixed clamp 701 to drive the T-shaped limiting slider 702 to slide inside the corresponding T-shaped slide groove 601, and at the same time drive the positioning gear 704 to mesh and roll on the surface of the positioning rack 602, until the fixed clamp 701 is lifted to a suitable height on the surface of the concave frame 6;

[0051] The fixing stud 810 is loosened and the limit plate 808 is lifted up inside the installation groove 801 under the rebound effect of the lifting spring 809, so that the movable connecting rod 807 drives the fixed clamping block 805 to move up inside the rectangular slide groove 803 and clamp it at the bottom of the positioning gear 704, so that the fixed clamping block 805 is meshed and clamped with the positioning gear 704, and the fixing nut 811 is tightened to lock and fix the fixing stud 810, so that the movable connecting rod 807 will not slide freely inside the installation groove 801, and the fixed clamping block 805 is clamped and fixed on the surface of the positioning gear 704 to clamp and fix the positioning gear 704, so that the positioning gear 704 is clamped and fixed. The movable rack 7 is fixed at a suitable height on the surface of the concave rack 6 and will not slide at will. Then the test tube 9 is inserted into the fixed clamp 701, so that the bottom end of the test tube 9 is pressed against the top of the elastic pad 603. The protective door panel 103 is rotated to separate from the first rubber pad 104 and press against the surface of the second rubber pad 105. The second rubber pad 105 is squeezed and deformed, so that the protective door panel 103 is clamped on the surface of the cell enrichment instrument body 1, and the inside of the cell enrichment instrument body 1 is shielded and protected. The cell enrichment instrument body 1 is started to process the tumor cells inside the test tube 9.

Claims

1. A fully automatic circulating tumor cell enrichment device, comprising a cell enrichment instrument body (1), characterized in that: A placement rack (2) is symmetrically rotatably mounted on one side of the cell enrichment instrument body (1), and a cleaning liquid storage box (3) and a waste liquid storage box (4) are respectively arranged inside the two groups of the placement racks (2), and the cleaning liquid storage box (3) and the waste liquid storage box (4) are connected to the cell enrichment instrument body (1) through a conduit. A fixed structure (5) is inlaid and fixedly mounted on the cell enrichment instrument body (1) at a central position between the two groups of the placement racks (2), and a concave rack (6) is fixedly mounted inside the cell enrichment instrument body (1), and a movable rack (7) is slidably mounted on one side of the concave rack (6), and a clamping block (8) is fixedly mounted on the bottom of the movable rack (7), and two groups of test tubes (9) are respectively clamped on both sides of the movable rack (7).

2. A fully automatic circulating tumor cell enrichment device according to claim 1, characterized in that: A placement groove (101) is symmetrically provided on one side of the cell enrichment instrument body (1), and arc-shaped limit grooves (102) are symmetrically provided on the bottom of both sides of the inner wall of the placement groove (101). A protective door plate (103) is symmetrically rotatably installed on one side of the cell enrichment instrument body (1) away from the placement groove (101). First rubber pads (104) are fixedly installed on the top and bottom of both sides of the cell enrichment instrument body (1), and the positions of the two groups of first rubber pads (104) correspond to each other. Second rubber pads (105) are fixedly installed at the central position of one side of the top and bottom of the cell enrichment instrument body (1), and the first rubber pad (104) and the second rubber pad (105) correspond to the positions of the protective door plate (103).

3. A fully automatic circulating tumor cell enrichment device according to claim 2, characterized in that: First limiting protrusions (201) are fixedly mounted on both sides of the placement rack (2), the first limiting protrusions (201) correspond to the positions of the arc-shaped limiting grooves (102), and the first limiting protrusions (201) are slidably connected inside the arc-shaped limiting grooves (102).

4. A fully automatic circulating tumor cell enrichment device according to claim 1, characterized in that: The fixed structure (5) comprises a circular mounting block (501), one side of the circular mounting block (501) is symmetrically provided with an arc-shaped slide groove (502), an arc-shaped slide rod (503) is fixedly installed inside the arc-shaped slide groove (502), a fixed clamping plate (504) is rotatably installed at a central position between two groups of arc-shaped slide grooves (502) on the surface of the circular mounting block (501), a hand-held ring (505) is fixedly installed at a central position of the fixed clamping plate (504) away from the circular mounting block (501), a sliding block (506) is symmetrically fixedly installed on one side of the fixed clamping plate (504) close to the circular mounting block (501), and the position of the sliding block (506) corresponds to the position of the arc-shaped slide groove (502), and the sliding block (506) is slidably connected to the surface of the arc-shaped slide rod (503), and an arc-shaped return spring (507) is provided on the surface of the arc-shaped slide rod (503) located on one side of the sliding block (506).

5. A fully automatic circulating tumor cell enrichment device according to claim 1, characterized in that: T-shaped slide grooves (601) are symmetrically arranged on both sides of the concave frame (6); a positioning rack (602) is fixedly installed at the central position inside the concave frame (6); an elastic pad (603) is fixedly installed on one side of the bottom of the concave frame (6); two supporting slots (604) are respectively arranged on both sides of the top of the elastic pad (603), and the supporting slots (604) correspond to the positions of the test tubes (9).

6. A fully automatic circulating tumor cell enrichment device according to claim 5, characterized in that: The movable frame (7) comprises two groups of fixed clamps (701), and the positions of the fixed clamps (701) and the supporting slots (604) correspond to each other; a T-shaped limiting slider (702) is fixedly mounted on one side of the fixed clamps (701); the T-shaped limiting slider (702) corresponds to the position of the T-shaped slide groove (601), and the T-shaped limiting slider (702) is slidably connected to the inside of the T-shaped slide groove (601); a mounting frame (703) is fixedly mounted between the two groups of fixed clamps (701); a positioning gear (704) is rotatably mounted inside the mounting frame (703), and the surface of the positioning gear (704) is meshed with the positioning rack (602).

7. A fully automatic circulating tumor cell enrichment device according to claim 1, characterized in that: The clamping block (8) is provided with an installation groove (801) inside, the bottom end of the installation groove (801) is connected to a sliding groove (802), the top end of the installation groove (801) is connected to a rectangular sliding groove (803), the inner walls of the rectangular sliding groove (803) are symmetrically provided with limiting sliding grooves (804), a fixed clamping block (805) is slidably installed inside the rectangular sliding groove (803), and second limiting protrusions (806) are symmetrically fixedly installed on both sides of the fixed clamping block (805), the second limiting protrusions (806) correspond to the positions of the limiting sliding grooves (804), and the second limiting protrusions (806) are slidably connected to the inside of the limiting sliding grooves (804).

8. A fully automatic circulating tumor cell enrichment device according to claim 7, characterized in that: A movable connecting rod (807) is fixedly installed at the bottom of the fixed block (805), and the bottom end of the movable connecting rod (807) passes through the installation groove (801) and extends to the sliding groove (802). A limit plate (808) is fixedly installed on the surface of the movable connecting rod (807) located at the top of the installation groove (801). A lifting spring (809) is provided on the surface of the movable connecting rod (807) located inside the installation groove (801). One end of the movable connecting rod (807) located inside the sliding groove (802) passes through the sliding groove (802) and is fixedly installed with a fixed stud (810), and a fixed nut (811) is provided on the surface of the fixed stud (810).

Images