Selenium-rich compound formula with anti-aging function for dogs

Abstract

The invention relates to the technical field of food science, and provides a selenium-rich compound formula with an anti-aging function for dogs. Comprising the following components: 5-12% of selenium-enriched yeast powder, 8-15% of compound probiotic freeze-dried powder, 25-30% of euphausia superba powder, 2-5% of a yucca extract, 4-8% of cranberry polyphenol, 5-10% of xylooligosaccharide, 10-15% of trehalose, 1-3% of carboxymethyl cellulose and 0.5-2% of phospholipid. The selenium-enriched yeast powder is used as a source of selenium, and the selenium exists in the form of selenomethionine, so that the bioavailability of the selenium is improved, and the toxicity risk is remarkably reduced. Compared with the prior art, the selenium-enriched yeast powder is added, so that the activity of an Nrf2 pathway in a dog body is up-regulated, the activity of superoxide dismutase (SOD) is further improved to 2.3 times of the original activity, free radicals are more efficiently removed, and senescence is delayed.

Description

Technical Field

[0001] The present invention relates to the field of food science and technology, and specifically, to a selenium-rich compound formula for dogs with anti-aging function. Background Art

[0002] With the upgrading of the concept of pet-raising, pet dogs are gradually regarded as family members, and people's demand for pet health is increasing day by day, especially the increasing attention to anti-aging. However, there are still many deficiencies in the functionality and safety of existing anti-aging foods for dogs. Most commercially available products use a single antioxidant component, such as vitamin E or glutathione. Although they can scavenge free radicals and reduce oxidative stress damage to a certain extent, they fail to effectively improve the imbalance of intestinal microecology, which is an important inducement for accelerating aging.

[0003] In addition, among the existing dog foods in the prior art, there are selenium supplements and probiotic powders:

[0004] Traditional selenium supplements (such as sodium selenite) have the problem of low bioavailability, with an actual absorption rate of only 40%, and have a relatively high toxicity risk (LD50 is 7mg / kg), and long-term use may lead to selenium poisoning.

[0005] For probiotic powders, during the high-temperature processing, the survival rate of probiotics is generally low. In many products using the extrusion process, the viable bacteria count is often less than 20%. This technical bottleneck seriously restricts the actual application effect of probiotics in dog foods. In view of this, the present invention proposes a selenium-rich compound formula for dogs with anti-aging function. Summary of the Invention

[0006] The present invention proposes a selenium-rich compound formula for dogs with anti-aging function, which solves the problems of low safety of selenium supplements and low activity of probiotic powders in the prior art.

[0007] The technical solution of the present invention is as follows: A selenium-rich compound formula for dogs with anti-aging function, calculated by mass percentage, includes the following components: selenium-rich yeast powder 5-12%, compound probiotic freeze-dried powder 8-15%, Antarctic krill powder 25-30%, yucca extract 2-5%, cranberry polyphenols 4-8%, xylo-oligosaccharide 5-10%, trehalose 10-15%, carboxymethyl cellulose 1-3%, phospholipid 0.5-2%.

[0008] Preferably, the compound probiotic includes Bifidobacterium animalis CP-9, Lactobacillus reuteri LR6 and Lactobacillus johnsonii LJ-23, and the mass ratio of the three components is 2:1:1.

[0009] Preferably, the preparation process of the selenium-rich compound formula for dogs with anti-aging function specifically comprises the following steps:

[0010] S1. Inoculate the compound probiotics into the MRS medium optimized with xylo-oligosaccharide, and anaerobically ferment at 38 °C for 24 hours;

[0011] S2. Centrifuge to collect the bacterial cells, then mix them with trehalose at a mass ratio of 1:1, and obtain the compound probiotic freeze-dried powder through vacuum freeze-drying;

[0012] S3. Through W / O / W double emulsion co-encapsulation, the inner aqueous phase contains compound probiotics and selenium-enriched yeast, the oil phase is phospholipid, and the outer aqueous phase is carboxymethyl cellulose solution. After high-pressure homogenization, stable microcapsules are formed;

[0013] S4. Mix the microcapsules in S3 with Antarctic krill powder, yucca extract, and cranberry polyphenols, and perform twin-screw extrusion granulation at 60 - 70 °C;

[0014] S5. Put the granules in S4 into a vacuum dryer and vacuum dry at 45 °C and -0.08 MPa until the water content ≤ 0.5%.

[0015] Preferably, in S1, the MRS medium comprises the following components: xylo-oligosaccharide (2% w / v), soy peptone 1.5%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.02%, manganese sulfate 0.005%.

[0016] Preferably, in S2, the specific implementation process of centrifuging to collect the bacterial cells is as follows:

[0017] A1. Aliquot the fermentation broth in S1 into centrifuge tubes, with each tube filled to ≤ 80% of the volume;

[0018] A2. Pre-cool the centrifuge rotor to 4 °C, then start the centrifuge and centrifuge at 8,000 × g for 15 minutes;

[0019] A3. Use a sterile pipette to aspirate the supernatant, and retain the precipitated bacterial sludge;

[0020] A4. Add physiological saline with a volume half of the original fermentation broth to the centrifuge tube, and gently stir with a sterile spatula until the bacterial sludge is completely suspended;

[0021] A5. Repeat centrifugation and washing 2 - 3 times, and take out the bacterial sludge to obtain the bacterial cells.

[0022] Preferably, in S2, the specific implementation process of vacuum freeze-drying is as follows:

[0023] B1. Spread the mixture of bacterial cells and trehalose on the freeze-drying tray, with a thickness ≤ 1 cm;

[0024] B2. Place the freeze-drying tray tiled with the mixture of bacteria and trehalose into the freeze dryer, and then start the pre-freezing program. Among them, the cooling rate is 5°C / min, the end temperature is -40°C, and the holding time is 2 - 2.5 hours to ensure that the moisture in the bacteria is completely converted into ice crystals;

[0025] B3. Sublimate the ice crystals under vacuum to remove the free water of the bacteria. Among them, the vacuum degree is 0.1 mbar, the baffle temperature is -35°C, and the holding time is 10 - 12 hours;

[0026] B4. Start the heating program, and the baffle temperature rises from -35°C to 25°C at a rate of 0.5°C / min. Among them, the vacuum degree is 0.05 mbar, and the holding time is 8 - 12 hours;

[0027] B5. Slowly fill the freeze-drying chamber with sterile nitrogen to normal pressure, and then take samples for testing to ensure that the viable bacteria count ≥ 1×10^10 CFU / g and the water content ≤ 5%.

[0028] Preferably, in S3, the specific implementation process of the W / O / W double emulsion co-embedding is as follows:

[0029] C1. Mix the freeze-dried powder of compound probiotics and selenium-enriched yeast powder, add PBS solution containing 5% trehalose, and then magnetically stir until completely dissolved to obtain the inner aqueous phase W1;

[0030] C2. Heat the medium-chain triglyceride to 50°C, then add soy lecithin. The mass ratio of medium-chain triglyceride to soy lecithin is 89:10, and magnetically stir until completely dissolved to obtain the oil phase O, and then cool to 30°C for standby;

[0031] C3. Slowly drop the inner aqueous phase W1 into the oil phase O, magnetically stir at 500 rpm for 5 minutes, and then homogenize under high pressure at 30 MPa to obtain the prepared W / O emulsion;

[0032] C4. Dissolve 2% sodium carboxymethylcellulose and 0.5% sodium alginate in deionized water to obtain the outer aqueous phase W2, and cool to 25°C after sterilization;

[0033] C5. Slowly add the W / O emulsion to the outer aqueous phase W2, magnetically stir at 300 rpm for 10 minutes, and then homogenize under high pressure at 15 MPa to form the W / O / W emulsion;

[0034] C6. Drop the W / O / W emulsion into the 2% CaCl2 solution. The volume ratio of the W / O / W emulsion to the 2% CaCl2 solution is 1:10, and magnetically stir at 200 rpm for 30 minutes. Sodium alginate and Ca 2 + forms a gel film to obtain the microcapsule.

[0035] Preferably, in S4, the specific implementation process of the twin-screw extrusion granulation is as follows:

[0036] D1. Dry-mix the microcapsules, Antarctic krill powder, yucca extract, and cranberry polyphenols for 5 minutes to ensure uniform dispersion, then spray 8-12% deionized water and continue mixing until the material is in a wet and agglomerated state;

[0037] D2. Uniformly feed the mixture into the inlet of the extruder through a loss-in-weight feeder, and then mix and compress it through the screw. Among them, the temperature of the mixing section is 35°C, and the temperature of the compression section is 40°C;

[0038] D3. The molten material is extruded through a porous die at 45°C to form a continuous strip, and then cooled to 25°C by an air-cooled conveyor belt and cut into 1-3 mm particles by a rotating blade.

[0039] The working principle and beneficial effects of the present invention are as follows:

[0040] 1. The present invention uses selenium-enriched yeast powder as the source of selenium, in which selenium exists in the form of selenomethionine, which not only improves the biological utilization rate of selenium but also significantly reduces the toxicity risk. Compared with the prior art, the addition of selenium-enriched yeast powder upregulates the activity of the Nrf2 pathway in dogs, and further increases the activity of superoxide dismutase (SOD) to 2.3 times the original level, thus more efficiently scavenging free radicals and delaying aging;

[0041] 2. Through the W / O / W double emulsion encapsulation technology, the survival rate of probiotics in simulated gastric juice is ≥80% (compared with ≤0.002% of non-encapsulated ones), and live bacteria are continuously released in intestinal juice (the final survival rate is 56%). The composite probiotics (Bifidobacterium CP-9, Lactobacillus reuteri LR6, Lactobacillus johnsonii LJ-23) synergistically proliferate, and the proportion of intestinal Bifidobacterium in the experimental group reaches 19.2%, significantly optimizing the intestinal microecology;

[0042] 3. Cranberry polyphenols scavenge free radicals and activate the Nrf2 pathway, increasing the serum SOD activity to 65.8 U / mL. Yucca extract inhibits the NF-κB pathway, reduces inflammation, and promotes the skin collagen density to increase to 75.9%. Antarctic krill powder provides ω-3 fatty acids and astaxanthin, synergistically delaying the aging of multiple organs;

[0043] 4. The microcapsule structure realizes the slow release of selenium, and the targeted release rate in intestinal juice reaches 95%, reducing gastric loss and improving the utilization rate of ingredients;

[0044] 5. Xylo-oligosaccharides optimize the culture medium, vacuum freeze-drying process, and twin-screw low-temperature extrusion granulation (60-70°C) to avoid high-temperature damage to active ingredients. The water content of the granules is ≤0.5%, extending the shelf life. Specific implementation mode

[0045] Next, in combination with the embodiments of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of protection of the present invention.

[0046] Embodiment 1:

[0047] This embodiment provides a selenium-rich compound formula for dogs with anti-aging function. Calculated by mass percentage, it includes the following components: selenium-enriched yeast powder 5%, compound probiotic freeze-dried powder (Bifidobacterium animalis subsp. lactis CP-9, Lactobacillus reuteri LR6, and Lactobacillus johnsonii LJ-23 with a mass ratio of 2:1:1) 8%, Antarctic krill powder 25%, yucca schidigera extract 2%, cranberry polyphenols 4%, xylooligosaccharide 5%, trehalose 10%, carboxymethyl cellulose 1%, phospholipid 0.5%.

[0048] The present invention uses selenium-enriched yeast powder as the source of selenium, in which selenium exists in the form of selenomethionine, which not only improves the biological utilization rate of selenium but also significantly reduces the toxicity risk. Compared with the prior art, the addition of selenium-enriched yeast powder upregulates the activity of the Nrf2 pathway in dogs, thereby increasing the activity of superoxide dismutase (SOD) to 2.3 times the original, so as to more efficiently scavenge free radicals and delay aging.

[0049] Embodiment 2:

[0050] This embodiment provides a selenium-rich compound formula for dogs with anti-aging function. Calculated by mass percentage, it includes the following components: selenium-enriched yeast powder 10%, compound probiotic freeze-dried powder (Bifidobacterium animalis subsp. lactis CP-9, Lactobacillus reuteri LR6, and Lactobacillus johnsonii LJ-23 with a mass ratio of 2:1:1) 10%, Antarctic krill powder 28%, yucca schidigera extract 4%, cranberry polyphenols 6%, xylooligosaccharide 7%, trehalose 13%, carboxymethyl cellulose 2%, phospholipid 1%.

[0051] Embodiment 3:

[0052] This embodiment provides a selenium-rich compound formula for dogs with anti-aging function. Calculated by mass percentage, it includes the following components: selenium-enriched yeast powder 12%, compound probiotic freeze-dried powder (Bifidobacterium animalis subsp. lactis CP-9, Lactobacillus reuteri LR6, and Lactobacillus johnsonii LJ-23 with a mass ratio of 2:1:1) 15%, Antarctic krill powder 30%, yucca schidigera extract 5%, cranberry polyphenols 8%, xylooligosaccharide 10%, trehalose 15%, carboxymethyl cellulose 3%, phospholipid 2%.

[0053] Embodiment 4:

[0054] This embodiment presents a preparation process for a selenium-rich compound formula for dogs with anti-aging function, and the specific steps are as follows:

[0055] S1. Inoculate the compound probiotics into the MRS medium optimized with xylooligosaccharide, and anaerobically ferment at 38 °C for 24 hours;

[0056] S2. Centrifuge to collect the bacterial cells, then mix them with trehalose at a mass ratio of 1:1, and then obtain the compound probiotic freeze-dried powder through vacuum freeze-drying;

[0057] S3. Through W / O / W double emulsion co-encapsulation, the inner aqueous phase contains compound probiotics and selenium-rich yeast, the oil phase is phospholipid, and the outer aqueous phase is carboxymethyl cellulose solution. After high-pressure homogenization, stable microcapsules are formed;

[0058] S4. Mix the microcapsules in S3 with Antarctic krill powder, yucca extract, and cranberry polyphenols, and carry out twin-screw extrusion granulation at 60 °C;

[0059] S5. Put the granules in S4 into a vacuum dryer and vacuum dry at 45 °C and -0.08 MPa until the water content ≤ 0.5%.

[0060] Comparative Example 1:

[0061] This comparative example presents a selenium-rich formula for dogs. The only difference from Example 1 is that the selenium-rich yeast powder is replaced with sodium selenite;

[0062] Comparative Example 2:

[0063] This comparative example presents a formula for dogs with anti-aging function. The only difference from Example 1 is that the yucca extract and cranberry polyphenols are replaced with vitamin E;

[0064] Comparative Example 3:

[0065] This comparative example presents a preparation process for a selenium-rich compound formula for dogs with anti-aging function, which is basically the same as the steps of Example 4. The only difference is that the compound probiotics and selenium-rich yeast are not encapsulated by the W / O / W emulsion method, but directly mixed with Antarctic krill powder, yucca extract, and cranberry polyphenols for low-temperature extrusion granulation;

[0066] Test Example 1:

[0067] This test example is used to detect the selenium utilization rate of dogs after eating the formula foods of Example 1 and Comparative Example 1. The specific implementation process is as follows:

[0068] (1). Select healthy adult beagle dogs (weighing 10 - 15 kg) and divide them into three groups, with 8 dogs in each group;

[0069] (2) Two groups were respectively fed the formulated foods of Example 1 and Comparative Example 1, and another group was used as Blank Group 1 and fed the basal diet (selenium content ≤ 0.1 mg / kg). They were accurately fed daily according to the body weight (0.3 mg Se / kg BW) to ensure that the selenium intakes of the experimental groups fed with the foods of Example 1 and Comparative Example 1 were the same.

[0070] (3) Then, blood selenium concentration, liver selenium content, kidney selenium content and fecal selenium content were sampled and detected on days 0, 7, 14 and 28 respectively. The specific detection results are shown in the following table.

[0071]

[0072] As can be seen from the above table, the blood selenium concentration of the experimental group fed with the food of Example 1 (selenium-enriched yeast powder) increased rapidly, reaching 130 μg / L on the 28th day (73% higher than the sodium selenite group). It had a long metabolic half-life and showed a sustained-release characteristic. The total selenium excretion amount only accounted for 15 - 20% of the intake, indicating that the biological utilization rate was as high as 80 - 85%. Thus, it can be known that the biological utilization rate of selenium in the experimental group fed with the food of Example 1 (selenium-enriched yeast powder) was significantly higher than that of the experimental group fed with the food of Comparative Example 1 (sodium selenite). In addition, due to the higher toxicity of inorganic selenium in the sodium selenite group, the selenium accumulation amount in the kidneys was still significantly lower than that in the selenium-enriched yeast group (2.1 vs 3.5 μg / g). Therefore, it was judged that the food of Example 1 (selenium-enriched yeast powder) had low toxicity and higher safety.

[0073] Test Example 2:

[0074] This test example was used to detect the anti-aging effect of dogs after eating the formulated foods of Example 1 and Comparative Example 2. The specific implementation process was as follows:

[0075] (1) Elderly dogs (7 - 10 years old) were selected and divided into three groups, with 10 dogs in each group.

[0076] (2) Two of the groups were respectively fed the formulated foods of Example 1 and Comparative Example 2, and another group was used as Blank Group 2 and fed the basal dog food (without anti-aging ingredients).

[0077] (3) After 12 weeks of feeding, blood samples were taken respectively to detect the SOD activity, skin collagen density and intestinal Bifidobacterium proportion. The detection results are shown in the following table.

[0078]

[0079] As can be seen from the above table, the SOD activity, skin collagen density and intestinal Bifidobacterium proportion in the experimental group fed with the formulated food of Example 1 all maintained relatively high levels. Among them, yucca extract reduced inflammation by inhibiting the NF-κB pathway and promoted the proliferation of intestinal Bifidobacterium. Cranberry polyphenols: scavenged free radicals and activated the Nrf2 pathway (SOD↑37%). Therefore, it can be judged that the formulated food of Example 1 could significantly delay the aging of multiple organs.

[0080] Test Example 3:

[0081] This test example is used to detect the protective effect of the foods prepared in Example 4 and Comparative Example 3 in gastric juice. The specific implementation process is as follows:

[0082] (1) Respectively place equal amounts of the foods prepared in Example 4 and Comparative Example 3 in simulated gastric fluid (SGF), oscillate at 37 °C (100 rpm) for 2 hours, and take samples for detection every 30 minutes;

[0083] (2) Then detect the viable bacteria count by the plate counting method and detect the selenium content in the dissolution solution by ICP-MS, and calculate the gastric selenium release rate.

[0084] The test results are as follows in the table;

[0085]

[0086] As can be seen from the above table, in the gastric juice stage, the viable bacteria survival rate of the food prepared in Example 4 ≥ 80%, while the viable bacteria survival rate of the unembedded food prepared in Comparative Example 3 ≤ 0.002%. Due to the phospholipid bilayer barrier of the W / O / W emulsion blocking gastric acid and pepsin, the selenium release rate of the food prepared in Example 4 is only 10%, while the unembedded food prepared in Comparative Example 3 releases 75% due to direct exposure. This shows that the W / O / W embedding technology significantly improves the gastric acid tolerance of probiotics, and at the same time, selenium has good release characteristics and can improve the bioavailability of selenium.

[0087] Test Example 4:

[0088] This test example is used to detect the protective effect of the foods prepared in Example 4 and Comparative Example 3 in intestinal fluid. The specific implementation process is as follows:

[0089] (1) Respectively place equal amounts of the foods prepared in Example 4 and Comparative Example 3 in simulated intestinal fluid (SIF), oscillate at 37 °C (100 rpm) for 4 hours, and take samples for detection every 1 hour;

[0090] (2) Then detect the viable bacteria count by the plate counting method and detect the selenium content in the dissolution solution by ICP-MS, and calculate the intestinal selenium release rate, which is calculated by the following formula:

[0091] The test results are as follows in the table;

[0092]

[0093] As can be seen from the above table, in the intestinal fluid stage, the food prepared in Example 4 continuously releases viable bacteria (final survival rate 56%), while the viable bacteria of the unembedded food prepared in Comparative Example 3 are completely inactivated in the gastric juice stage (≤ 1.0×102 CFU / g). In addition, for the food prepared in Example 4, under the action of intestinal fluid pH (6.8) and bile salts, the outer oil phase dissolves, and 85-95% of selenium is target-released within 2-4 hours, which is consistent with the final release rate of the food prepared in Comparative Example 3 without embedding, but avoids the loss caused by premature release in the stomach.

[0094] The above are only the preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A selenium-rich composite formula for dogs with anti-aging function, characterized in that: Calculated by mass percentage, the invention includes the following ingredients: 5-12% selenium-enriched yeast powder, 8-15% compound probiotic freeze-dried powder, 25-30% Antarctic krill powder, 2-5% yucca extract, 4-8% cranberry polyphenols, 5-10% xylo-oligosaccharides, 10-15% trehalose, 1-3% carboxymethyl cellulose and 0.5-2% phospholipids.

2. The selenium-rich composite formula for dogs with anti-aging function according to claim 1, characterized in that: The composite probiotics include Bifidobacterium animalis CP-9, Lactobacillus reuteri LR6 and Lactobacillus johnsonii LJ-23, and the mass ratio of the three components is 2:1:

1.

3. The selenium-rich composite formula for dogs with anti-aging function according to claim 2, characterized in that: The specific steps of the preparation process of the selenium-rich composite formula for dogs with anti-aging function are as follows: S1. Inoculate the composite probiotics into the MRS medium containing optimized xylooligosaccharide and perform anaerobically fermentation at 38°C for 24 hours; S2, collecting the bacterial cells by centrifugation, then mixing them with trehalose at a mass ratio of 1:1, and then performing vacuum freeze drying to obtain a composite probiotic freeze-dried powder; S3, co-encapsulation by W / O / W double emulsion, the inner aqueous phase contains compound probiotics and selenium-enriched yeast, the oil phase is phospholipids, the outer aqueous phase is carboxymethyl cellulose solution, and stable microcapsules are formed after high-pressure homogenization; S4, mixing the microcapsules in S3 with Antarctic krill powder, yucca extract, and cranberry polyphenols, and granulating by twin-screw extrusion at 60-70°C; S5. Place the particles in S4 into a vacuum dryer and vacuum dry at 45° C. and -0.08 MPa until the moisture content is ≤0.5%.

4. The selenium-rich composite formula for dogs with anti-aging function according to claim 3, characterized in that: In S1, the MRS medium includes the following components: xylooligosaccharide (2% w / v), soy peptone 1.5%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.02%, and manganese sulfate 0.005%.

5. The selenium-rich composite formula for dogs with anti-aging function according to claim 3, characterized in that: In S2, the specific implementation process of collecting bacterial cells by centrifugation is as follows: A1, dispense the fermentation liquid in S1 into centrifuge tubes, with each tube filled ≤ 80% of the volume; A2. Precool the centrifuge rotor to 4°C, then start the centrifuge at 8,000×g for 15 minutes. A3. Use a sterile pipette to remove the supernatant and retain the precipitated bacterial sludge; A4. Add half the volume of the fermentation solution in normal saline to the centrifuge tube and stir gently with a sterile spatula until the bacterial sludge is completely suspended; A5. Repeat centrifugation and washing 2-3 times, and take out the bacterial sludge to obtain the bacterial cells.

6. The selenium-rich composite formula for dogs with anti-aging function according to claim 3, characterized in that: In S2, the specific implementation process of the vacuum freeze drying is as follows: B1. Spread the mixture of bacteria and trehalose on a freeze-drying tray with a thickness of ≤1 cm; B2. Place the freeze-drying tray with the mixture of bacteria and trehalose into the freeze dryer, and then start the pre-freezing program, wherein the cooling rate is 5°C / min, the end temperature is -40°C, and the maintenance time is 2-2.5 hours to ensure that the moisture of the bacteria is completely converted into ice crystals; B3, subliming ice crystals to remove free water from the bacteria under vacuum, wherein the vacuum degree is 0.1 mbar, the partition temperature is -35°C, and the maintenance time is 10-12 hours; B4. Start the temperature rising program, the temperature of the partition rises from -35°C to 25°C at 0.5°C / min, the vacuum degree is 0.05mbar, and the maintenance time is 8-12 hours; B5. Slowly fill the freeze-drying chamber with sterile nitrogen to normal pressure, then take samples for testing to ensure that the number of viable bacteria is ≥1×10^10 CFU / g and the water content is ≤5%.

7. The selenium-rich composite formula for dogs with anti-aging function according to claim 3, characterized in that: In S3, the specific implementation process of the W / O / W double emulsion co-embedding is as follows: C1, mixing the composite probiotic freeze-dried powder with selenium-enriched yeast powder, adding a PBS solution containing 5% trehalose, and then stirring by magnetic force until completely dissolved to obtain an inner water phase W1; C2, heat the medium chain triglyceride to 50°C, then add soybean lecithin, the mass ratio of medium chain triglyceride to soybean lecithin is 89:10, stir magnetically to completely dissolve to obtain oil phase O, and then cool to 30°C for use; C3, slowly drop the inner water phase W1 into the oil phase O, magnetically stir at 500 rpm for 5 minutes, and then homogenize under high pressure at 30 MPa to prepare a W / O emulsion; C4, dissolving 2% sodium carboxymethyl cellulose and 0.5% sodium alginate in deionized water to obtain an external aqueous phase W2, sterilizing and cooling to 25°C; C5, slowly adding the W / O emulsion into the external aqueous phase W2, stirring magnetically at 300 rpm for 10 minutes, and then high-pressure homogenizing at 15 MPa to form a W / O / W emulsion; C6. Add the W / O / W emulsion dropwise into the 2% CaCl2 solution. The volume ratio of the W / O / W emulsion to the 2% CaCl2 solution is 1:

10. Stir magnetically at 200 rpm for 30 minutes. 2 + Form a gel film to obtain microcapsules.

8. The selenium-rich composite formula for dogs with anti-aging function according to claim 3, characterized in that: In S4, the specific implementation process of the twin-screw extrusion granulation is as follows: D1. Dry mix the microcapsules, Antarctic krill powder, yucca extract, and cranberry polyphenols for 5 minutes to ensure uniform dispersion, then spray 8-12% deionized water and continue mixing until the materials are in a wet agglomerated state; D2, the mixed material is uniformly fed into the feed port of the extruder through a loss-in-weight feeder, and then mixed and compressed by a screw, wherein the temperature of the mixing section is 35°C and the temperature of the compression section is 40°C; D3. The molten material is extruded through a porous die at 45°C to form a continuous strip, then cooled to 25°C by an air-cooled conveyor belt and cut into 1-3 mm particles by a rotating blade.

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