Traditional Chinese medicine composition for treating HPV infection after conization and preparation method and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for treating HPV infection after conization and a preparation method and application thereof. The traditional Chinese medicine composition comprises the following raw materials: raw radix astragali, roasted rhizoma atractylodis macrocephalae, codonopsis pilosula, radix bupleuri, rhizoma cyperi, gordon euryale seed, ginkgo, cortex phellodendri, semen plantaginis, angelica sinensis, salvia chinensis, honey-fried licorice root, polygonum cuspidatum, edible tulip and receptaculum nelumbinis, the compatibility of the whole formula is proper, spleen qi is vigorous, damp turbidity is transformed, pathogenic heat is cleared, and stasis is eliminated, and the traditional Chinese medicine composition has a good treatment effect on HPV infection after conization. Virus infection can be prevented from developing into precancerous lesions of cervical cancer again or even developing into cervical cancer.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for treating HPV infection after conization, a preparation method thereof, and an application thereof. Background Art

[0002] Cervical cancer is one of the common malignant tumors in gynecology. Generally speaking, cervical precancerous lesions are an inevitable process in the occurrence of cervical cancer. Cervical precancerous lesions refer to cervical intraepithelial neoplasia and cervical carcinoma in situ, which belong to benign lesions. Most of the cells show atypical hyperplasia, without qualitative changes and without showing the characteristics of cancer cells. 31-50% of untreated patients with cervical precancerous lesions can progress to cervical cancer. However, it usually takes about 10 years for cervical precancerous lesions to develop into cervical cancer. Therefore, cervical precancerous lesions should be treated in a timely manner through drugs, surgical resection, etc. to prevent the development of cervical precancerous lesions and thus truncate the process of cervical cancer.

[0003] HPV (human papillomavirus) infection is one of the main causes of cervical precancerous lesions. If not treated in a timely and effective manner, it may progress to cervical precancerous lesions. When patients with HPV infection develop into cervical precancerous lesions, resection treatment needs to be performed locally on the cervix. However, surgical resection can only remove the local lesion site and cannot clear the virus infection. If the virus persists in infection, it may develop into cervical precancerous lesions again or even progress to cervical cancer. The main treatment methods for cervical precancerous lesions are drug treatment and surgical treatment. For low-grade intraepithelial lesions, drugs such as interferon can be used for treatment or laser treatment can be adopted. However, interferon has adverse reactions such as fever, headache, decreased white blood cell count, and increased transaminase. If the above methods cannot effectively control the development of the disease and progress to high-grade intraepithelial lesions, surgical treatment is required. Cervical resection treatment is the standardized treatment plan for cervical precancerous lesions recommended by the guidelines. However, surgical treatment has the risk of causing surgical site infection, only solves the local lesion, there is still a possibility of residual lesions, and it cannot clear the HPV virus infection in the patient's body. Therefore, it is particularly important to prevent HPV infection from progressing to cervical precancerous lesions. Summary of the Invention

[0004] In view of the above technical problems, the present invention provides a traditional Chinese medicine composition for treating HPV infection after conization, a preparation method thereof, and an application thereof. The traditional Chinese medicine composition can effectively treat HPV infection after conization, thereby preventing the virus infection from progressing to cervical precancerous lesions or even progressing to cervical cancer.

[0005] To achieve the above invention purpose, the present invention adopts the following technical solutions: On the one hand, the present invention provides a traditional Chinese medicine composition for treating HPV infection after conization, and the raw materials are proportioned by mass as follows: 24-36 parts of astragalus membranaceus, 8-12 parts of stir-fried atractylodes macrocephala, 12-18 parts of codonopsis pilosula, 5-7 parts of bupleurum chinense, 8-12 parts of cyperus rotundus, 12-18 parts of euryale ferox, 8-12 parts of ginkgo biloba, 5-7 parts of phellodendron amurense, 8-12 parts of plantago asiatica, 5-7 parts of angelica sinensis, 12-18 parts of salvia chinensis, 5-7 parts of roasted licorice root, 12-18 parts of polygonum cuspidatum, 4-6 parts of pleione bulbocodioides, and 8-12 parts of lotus receptacle.

[0006] Astragalus membranaceus is warm in nature, sweet in taste, and belongs to the lung and spleen meridians; it supplements qi and consolidates the exterior, promotes diuresis and expels toxins, discharges pus, and heals sores and generates flesh. Stir-fried atractylodes macrocephala is warm in nature, bitter and sweet in taste, and belongs to the spleen and stomach meridians; it supplements qi and strengthens the spleen, dries dampness and promotes diuresis, and stops sweating. Codonopsis pilosula is neutral in nature, sweet in taste, and belongs to the spleen and lung meridians; it replenishes the middle-jiao and benefits qi, quenches thirst, strengthens the spleen and nourishes the lung, and nourishes blood and promotes fluid production. Bupleurum chinense is slightly cold in nature, bitter and pungent in taste, and belongs to the liver, gallbladder, and lung meridians; it relieves exterior syndrome and dispels cold, soothes the liver and relieves depression, raises yang and lifts the sinking, and expels malaria and reduces fever. Cyperus rotundus is neutral in nature, pungent, slightly bitter, and slightly sweet in taste, and belongs to the liver, spleen, and triple energizer meridians; it soothes the liver and relieves depression, regulates menstruation and alleviates pain, and regulates qi and harmonizes the middle-jiao. Euryale ferox is neutral in nature, sweet and astringent in taste, and belongs to the spleen and kidney meridians; it tonifies the kidney and consolidates essence, strengthens the spleen and stops diarrhea, and eliminates dampness and stops leukorrhea. Ginkgo biloba is warm in nature, sweet, slightly bitter and astringent in taste, slightly poisonous, and belongs to the lung and kidney meridians; it astringes the lung and relieves asthma, stops leukorrhea and reduces urination. Phellodendron amurense is cold in nature, bitter in taste, and belongs to the kidney, bladder, and large intestine meridians; it clears heat and dries dampness, purges fire and removes steaming, and detoxifies and treats sores. Plantago asiatica is cold in nature, sweet in taste, and belongs to the liver, kidney, lung, and small intestine meridians; it clears heat and promotes diuresis, percolates dampness and stops diarrhea, clears the liver and improves eyesight, and reduces phlegm and relieves cough. Angelica sinensis is warm in nature, sweet and pungent in taste, and belongs to the liver, heart, and large intestine meridians; it nourishes blood and regulates menstruation, promotes blood circulation and alleviates pain, and moistens the intestine and promotes defecation. Salvia chinensis is slightly cold in nature, pungent and bitter in taste, and belongs to the liver and spleen meridians; it promotes blood circulation to remove stasis, clears heat and promotes diuresis, and dissipates stasis and reduces swelling. Roasted licorice root is neutral in nature, sweet in taste, and belongs to the heart, lung, spleen, and stomach meridians; it invigorates the spleen and replenishes qi, clears heat and detoxifies, reduces phlegm and relieves cough, alleviates spasm and pain, and harmonizes various herbs. Polygonum cuspidatum is slightly cold in nature, slightly bitter in taste, and belongs to the liver, gallbladder, and lung meridians; it promotes diuresis and reduces jaundice, clears heat and detoxifies, disperses stasis and relieves pain, and reduces phlegm and relieves cough. Pleione bulbocodioides is cool in nature, sweet and slightly pungent in taste, and belongs to the liver and spleen meridians; it clears heat and detoxifies, dissipates carbuncles and reduces masses. Lotus receptacle is warm in nature, bitter and astringent in taste, and belongs to the liver meridian; it dissipates stasis, stops bleeding, and removes dampness.

[0007] In this Chinese medicine composition, Astragalus, Codonopsis and Atractylodes all have the function of nourishing and strengthening the body. Astragalus is sweet and warm, and can nourish the middle qi; Codonopsis is sweet and mild, and enters the lungs and spleen. In addition to nourishing qi, it can also nourish blood and produce body fluid. "Ben Cao Zheng Yi" records that this medicine "is particularly valuable, because it can strengthen the spleen without being dry, and nourish the stomach yin without being damp"; Atractylodes is good at nourishing qi, strengthening the spleen and eliminating dampness, and is often fried in the prescription to enhance its spleen-tonifying power; the three are used together to nourish the deficiency without being greasy. Bupleurum can dissipate and raise yang, and Ginkgo can astringe and lower qi. The two medicines are used together to dissipate and astringe qi, so that the liver qi can be dispersed and the spleen qi can be strong. "Ben Cao Jing Shu" says: "Cyperus rotundus can treat women with leucorrhea and irregular menstruation, all of which can lower qi, regulate qi, disperse knots, and regulate stagnation." The combined use of Cyperus rotundus to regulate qi and relieve depression can enhance the function of regulating liver qi. Huangbai clears kidney fire to relieve the heat of Ren channel; Plantago seed clears heat and promotes diuresis to penetrate dampness and resolve turbidity; Polygonum cuspidatum clears heat and promotes diuresis, activates blood circulation and dredges the meridians, which not only eliminates dampness and heat to allow the evil qi to have a way out, but also promotes diuresis to remove blood stasis, resolves accumulation and disperses nodules. Gorgon fruit is sweet and astringent, which can replenish the deficiency of Ren channel, remove dampness and stop leukorrhea; with Angelica sinensis, Lithospermum officinale, Trichosanthes kirilowii and Lotus pod, Angelica sinensis harmonizes blood without hurting the body. The "Compendium of Materia Medica" says that it "treats Chong channel and Dai channel diseases, and is a blood-qi medicine, which can clear blood stasis, replenish blood deficiency, moisten blood dryness, and soothe blood disorder, so that qi and blood can each have their own place, dissipate internal cold, replenish deficiency, remove blood stasis and generate new blood"; Lithospermum officinale is bitter and pungent, and has strong ability to clear heat, promote blood circulation and disperse nodules. The combination of the two medicines can achieve the effect of eliminating symptoms and dispersing nodules; the mountain aconite has strong heat-clearing and detoxifying power, and is good at eliminating carbuncle and dispersing nodules; the lotus pod is warm and astringent, and can eliminate blood stasis, remove dampness, and resist cancer toxins. The Compendium of Materia Medica says that this medicine "eliminates blood stasis and disperses blood", and the Wuling Materia Medica says that this medicine "burns ash to stop metrorrhagia, fetal leakage, hematuria, etc." The four medicines can clear heat, activate blood circulation and dissipate blood stasis, remove old and rotten things, and can dissipate symptoms, and also harmonize blood and promote body recovery; this is because "women are born with liver", and the Lingshu says: "The liver foot Jueyin meridian starts at the tuft of hair on the big toe... along the thigh pubic region, enter the hair, circle the genitals, and reach the lower abdomen", and the foot Jueyin liver meridian runs close to the cervix, which is closely related to leucorrhea. Therefore, the prescription compatibility selects Cyperus rotundus, Polygonum cuspidatum, Lithospermum officinale, mountain aconite, and lotus pod to enter the foot Jueyin liver meridian, which can help the medicine reach the diseased area directly. Add roasted licorice to harmonize the medicines. The whole formula is properly matched to strengthen the spleen, transform dampness and turbidity, clear away evil heat, and eliminate symptoms and accumulations.

[0008] The Chinese medicine composition eliminates the virus by improving the body's immunity, preventing persistent viral infection from developing into cervical precancerous lesions or even progressing into cervical cancer. Clinical efficacy observation data proves that compared with the existing commonly used drug human interferon α-2b, the Chinese medicine composition of the present invention can more effectively treat persistent HPV infection after conization, and has significant differences, and can more effectively prevent viral infection from developing into cervical precancerous lesions or even progressing into cervical cancer.

[0009] Preferably, the raw materials of the traditional Chinese medicine composition are proportioned by mass parts and include: 27-33 parts of Astragalus membranaceus, 9-11 parts of stir-fried Atractylodes macrocephala, 13.5-16.5 parts of Codonopsis pilosula, 5.5-6.5 parts of Bupleurum chinense, 9-11 parts of Cyperus rotundus, 13.5-16.5 parts of Euryale ferox, 9-11 parts of Ginkgo biloba, 5.5-6.5 parts of Phellodendron amurense, 9-11 parts of Plantago asiatica, 5.5-6.5 parts of Angelica sinensis, 13.5-16.5 parts of Salvia chinensis, 5.5-6.5 parts of roasted Glycyrrhiza uralensis, 13.5-16.5 parts of Polygonum cuspidatum, 4.5-5.5 parts of Cremastra appendiculata, and 9-11 parts of Nelumbo nucifera receptacle.

[0010] Preferably, the raw materials of the traditional Chinese medicine composition are proportioned by mass parts and include: 30 parts of Astragalus membranaceus, 10 parts of stir-fried Atractylodes macrocephala, 15 parts of Codonopsis pilosula, 6 parts of Bupleurum chinense, 10 parts of Cyperus rotundus, 15 parts of Euryale ferox, 10 parts of Ginkgo biloba, 6 parts of Phellodendron amurense, 10 parts of Plantago asiatica, 6 parts of Angelica sinensis, 15 parts of Salvia chinensis, 6 parts of roasted Glycyrrhiza uralensis, 15 parts of Polygonum cuspidatum, 5 parts of Cremastra appendiculata, and 10 parts of Nelumbo nucifera receptacle.

[0011] In actual clinical use, the composition is based on 1 g corresponding to each weight part, and 1-2 doses are used every day according to the condition.

[0012] Second, the present invention also provides a preparation method of the above traditional Chinese medicine composition: soak the above raw materials in water and then decoct and extract, and take the obtained water extract to obtain the composition.

[0013] Preferably, decoct and extract twice. First, bring to a boil over high heat, and then simmer over low heat for 30-40 min, filter, and take the filtrate; add water to the obtained medicinal residues, bring to a boil over high heat, and then simmer over low heat for 20-30 min, filter, and take the filtrate; combine the filtrates to obtain the composition. More preferably: first bring to a boil over high heat and then simmer over low heat for 35 min for the first time, and bring to a boil over high heat and then simmer over low heat for 25 min for the second time.

[0014] When decocting and extracting twice, the amount of water used for soaking should just cover the above raw materials.

[0015] Preferably, the water temperature for soaking is 30-45 °C, the soaking time is 20-40 min, and more preferably 30 min.

[0016] Preferably, the preparation method further includes concentrating and / or drying the obtained water extract for easy transportation, storage, and use.

[0017] Third, the present invention also provides the application of the above traditional Chinese medicine composition or the traditional Chinese medicine composition prepared by the above preparation method in the preparation of a drug for treating HPV infection after conization.

[0018] Preferably, the drug is an oral preparation, including but not limited to powder, granule, capsule, tablet, pill, oral liquid, etc.

[0019] Preferably, the above oral preparation further comprises a pharmaceutically acceptable excipient, such as a filler, a binder, a disintegrant, an anti-adhesive, a glidant, a flavoring agent, etc. Detailed implementation manners

[0020] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

[0021] Astragalus membranaceus, Codonopsis pilosula, Cyperus rotundus, Euryale ferox, Ginkgo biloba, Phellodendron amurense, Plantago asiatica, Angelica sinensis, Radix Glycyrrhizae Preparata, Polygonum cuspidatum, Cremastra appendiculata and Nymphaea tetragona used in the following examples all meet the requirements of each drug item in the Chinese Pharmacopoeia (Part I). Stir-fried Atractylodes macrocephala meets the requirements of each item under "Stir-fried Atractylodes macrocephala" in the Shaanxi Traditional Chinese Medicine Decoction Pieces Standard - Volume I, and Salvia chinensis meets the requirements of each item under "Salvia chinensis" in the Guangdong Chinese Medicinal Materials Standard - Volume III.

[0022] Example 1 An embodiment of the present invention provides a traditional Chinese medicine composition for treating HPV infection after conization. Each dose is made from the following raw materials: 30 g of Astragalus membranaceus, 10 g of stir-fried Atractylodes macrocephala, 15 g of Codonopsis pilosula, 6 g of Bupleurum chinense, 10 g of Cyperus rotundus, 15 g of Euryale ferox, 10 g of Ginkgo biloba, 6 g of Phellodendron amurense, 10 g of Plantago asiatica, 6 g of Angelica sinensis, 15 g of Salvia chinensis, 6 g of Radix Glycyrrhizae Preparata, 15 g of Polygonum cuspidatum, 5 g of Cremastra appendiculata and 10 g of Nymphaea tetragona.

[0023] Preparation method: Put the above raw materials into warm water (35 - 40 °C) for soaking, with the water covering the medicinal materials. Start decocting after 30 min: first bring to a boil over high heat, then simmer over low heat for 35 min, filter, and collect the filtrate; add water to cover the medicinal residues after decocting, bring to a boil over high heat, then simmer over low heat for 25 min, filter, and collect the filtrate; mix the filtrates obtained from the two decoctions to obtain the product.

[0024] Example 2 An embodiment of the present invention provides a traditional Chinese medicine composition for treating HPV infection after conization. Each dose is made from the following raw materials: 27 g of Astragalus membranaceus, 9 g of stir-fried Atractylodes macrocephala, 13.5 g of Codonopsis pilosula, 5.5 g of Bupleurum chinense, 9 g of Cyperus rotundus, 13.5 g of Euryale ferox, 9 g of Ginkgo biloba, 5.5 g of Phellodendron amurense, 9 g of Plantago asiatica, 5.5 g of Angelica sinensis, 13.5 g of Salvia chinensis, 5.5 g of Radix Glycyrrhizae Preparata, 13.5 g of Polygonum cuspidatum, 4.5 g of Cremastra appendiculata and 9 g of Nymphaea tetragona.

[0025] The preparation method is the same as that in Example 1.

[0026] Example 3 An embodiment of the present invention provides a traditional Chinese medicine composition for treating HPV infection after conization. Each dose is made from the following raw materials: 33 g of Astragalus membranaceus, 11 g of stir-fried Atractylodes macrocephala, 16.5 g of Codonopsis pilosula, 6.5 g of Bupleurum chinense, 11 g of Cyperus rotundus, 16.5 g of Euryale ferox, 11 g of Ginkgo biloba, 6.5 g of Phellodendron amurense, 11 g of Plantago asiatica, 6.5 g of Angelica sinensis, 16.5 g of Salvia chinensis, 6.5 g of roasted Glycyrrhiza uralensis, 16.5 g of Polygonum cuspidatum, 5.5 g of Cremastra appendiculata, and 11 g of Nelumbo nucifera receptacle.

[0027] The preparation method is the same as that in Example 1.

[0028] Example 4 An embodiment of the present invention provides a traditional Chinese medicine composition for treating HPV infection after conization. Each dose is made from the following raw materials: 24 g of Astragalus membranaceus, 8 g of stir-fried Atractylodes macrocephala, 12 g of Codonopsis pilosula, 5 g of Bupleurum chinense, 8 g of Cyperus rotundus, 12 g of Euryale ferox, 8 g of Ginkgo biloba, 5 g of Phellodendron amurense, 8 g of Plantago asiatica, 5 g of Angelica sinensis, 12 g of Salvia chinensis, 5 g of roasted Glycyrrhiza uralensis, 12 g of Polygonum cuspidatum, 4 g of Cremastra appendiculata, and 8 g of Nelumbo nucifera receptacle.

[0029] Preparation method: Put the above raw materials into warm water (30 - 35 °C) for soaking, with the water covering the medicinal materials. Start decocting after 40 min: first bring to a boil over high heat, then simmer over low heat for 30 min, filter, and collect the filtrate; add water to cover the medicinal residues after decocting, bring to a boil over high heat, then simmer over low heat for 30 min, filter, and collect the filtrate; mix the filtrates obtained from the two decoctions to obtain the product.

[0030] Example 5 An embodiment of the present invention provides a traditional Chinese medicine composition for treating HPV infection after conization. Each dose is made from the following raw materials: 36 g of Astragalus membranaceus, 12 g of stir-fried Atractylodes macrocephala, 18 g of Codonopsis pilosula, 7 g of Bupleurum chinense, 12 g of Cyperus rotundus, 18 g of Euryale ferox, 12 g of Ginkgo biloba, 7 g of Phellodendron amurense, 12 g of Plantago asiatica, 7 g of Angelica sinensis, 18 g of Salvia chinensis, 7 g of roasted Glycyrrhiza uralensis, 18 g of Polygonum cuspidatum, 6 g of Cremastra appendiculata, and 12 g of Nelumbo nucifera receptacle.

[0031] Preparation method: Put the above raw materials into warm water (40 - 45 °C) for soaking, with the water covering the medicinal materials. Start decocting after 20 min: first bring to a boil over high heat, then simmer over low heat for 40 min, filter, and collect the filtrate; add water to cover the medicinal residues after decocting, bring to a boil over high heat, then simmer over low heat for 20 min, filter, and collect the filtrate; mix the filtrates obtained from the two decoctions to obtain the product.

[0032] Effect Example 1 This effect example provides the statistical results of the clinical effect of the traditional Chinese medicine composition in Example 1 of the present invention in treating HPV infection after conization.

[0033] 1. Research subjects Case source: Patients with HPV infection after conization who visited the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine from October 2023 to October 2024, aged between 27 and 60 years old, a total of 72 cases.

[0034] 2. Treatment methods Treatment group: Orally take the traditional Chinese medicine composition in Example 1 of the present invention, one dose per day, taken separately in the morning and evening.

[0035] Control group: Given recombinant human interferon α-2b gel (Youjing'an) for external use.

[0036] One month is one course of treatment, and 3 courses of treatment are given.

[0037] 3. Efficacy evaluation The determination of HPV negative conversion efficacy is diagnosed in combination with the HPV reexamination results.

[0038] Cure: The originally infected HPV turns negative, and no new subtypes appear; Marked effect: At least one of the HPV subtypes detected turns negative, and no other types increase; Effective: The number of originally infected HPV subtypes decreases, but new types increase; Ineffective: The originally infected HPV subtype is still positive or new subtypes appear on the original basis.

[0039] Total effective rate = (cure + marked effect + effective) / total number of cases × 100% 4. Results The efficacy evaluation results of each group are shown in Table 1: Table 1 Results of efficacy and comparison between groups

[0040] Note: Compared with the effective rate of the control group, * P <0.05.

[0041] Clinical results show that: Compared with the existing Youjing'an, the traditional Chinese medicine composition provided by the present invention can more effectively treat HPV infection after conization, and the difference between the two is significant, indicating that the traditional Chinese medicine composition provided by the present invention can more effectively prevent the virus infection from developing into cervical intraepithelial neoplasia or even progressing to cervical cancer again.

[0042] Effect example 2 This effect example provides a typical case of treating HPV infection after conization with the traditional Chinese medicine composition in Example 1 of the present invention.

[0043] Li XX, female, 45 years old, married. Initial diagnosis on May 31, 2024. Increased leucorrhea for 3 years, slightly yellow in color. Examination findings: The patient had her first menstruation at the age of 14, with regular menstruation, a cycle of 30 days, a menstrual period of 3 - 4 days, moderate amount, dark red in color. She usually had soreness in the waist. The tongue was pale red with tooth marks, the tongue coating was slightly yellow, and the pulse was thready and weak. She had undergone HPV and TCT tests, and the results were HPV: 16, 18 + respectively, and TCT showed mild inflammation. The pathological examination of colposcopic cervical biopsy showed that at 12 o'clock of the cervix: partial squamous epithelium showed high - grade squamous intraepithelial lesion (CINⅡ - CINⅢ grade). After treatment, HPV typing test and TCT examination were regularly reviewed. Immunohistochemistry: P16 protein (+), Ki - 67 nuclear antigen scattered in basal and middle - layer cells - full - layer cells (+). At 3, 6, 9 o'clock of the cervix: chronic inflammation, squamous epithelial hyperplasia, vacuolar degeneration, and koilocytic cells were visible in focal intraepithelium. The scraping of the endocervical canal: The specimen was mucus and fragmented endocervical canal tissue, accompanied by inflammatory cell infiltration. Subsequently, the patient underwent cervical conization surgery, and the pathology showed multifocal high - grade squamous intraepithelial lesion (CINII - Ш grade) of the cervix. The resection margin was (-). HPV typing test and TCT examination were regularly reviewed. Immunohistochemistry: P16 protein (+), Ki - 67 nuclear antigen diffusely positive in all - layer squamous epithelium (+). Subsequently, the patient sought traditional Chinese medicine treatment and visited the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine. Western medicine diagnosis: Cervical intraepithelial neoplasia grade III. Traditional Chinese medicine diagnosis: Leukorrhea disease, syndrome of spleen deficiency and damp - heat. Treatment principle: Tonify the spleen and boost qi, clear heat and resolve dampness. Prescription: Treated with the traditional Chinese medicine composition provided in Example 1 of the present invention. One dose per day, taken separately in the morning and evening. After taking the medicine for 3 months, the medicine was stopped. On December 6, 2024, the re - examination of HPV showed (-), and both of the two high - risk types turned negative.

[0044] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A Chinese medicine composition for treating HPV infection after conization, characterized in that: The raw materials of the traditional Chinese medicine composition include, by weight, 24-36 parts of astragalus, 8-12 parts of stir-fried atractylodes, 12-18 parts of codonopsis, 5-7 parts of bupleurum, 8-12 parts of cyperus, 12-18 parts of euryale, 8-12 parts of ginkgo, 5-7 parts of phellodendron, 8-12 parts of plantain, 5-7 parts of angelica, 12-18 parts of lithospermum, 5-7 parts of roasted liquorice, 12-18 parts of knotweed, 4-6 parts of trillium and 8-12 parts of lotus seed pods.

2. The Chinese medicine composition according to claim 1, characterized in that: The raw materials of the traditional Chinese medicine composition include, by weight, 27-33 parts of astragalus, 9-11 parts of stir-fried atractylodes, 13.5-16.5 parts of codonopsis, 5.5-6.5 parts of bupleurum, 9-11 parts of cyperus, 13.5-16.5 parts of euryale, 9-11 parts of ginkgo, 5.5-6.5 parts of phellodendron, 9-11 parts of plantain, 5.5-6.5 parts of angelica, 13.5-16.5 parts of lithospermum, 5.5-6.5 parts of roasted liquorice, 13.5-16.5 parts of knotweed, 4.5-5.5 parts of trillium and 9-11 parts of lotus seed pods.

3. The Chinese medicine composition according to claim 2, characterized in that: The raw materials of the traditional Chinese medicine composition include, by mass, 30 parts of astragalus, 10 parts of stir-fried atractylodes, 15 parts of codonopsis, 6 parts of bupleurum, 10 parts of cyperus, 15 parts of euryale, 10 parts of ginkgo, 6 parts of phellodendron, 10 parts of plantain seeds, 6 parts of angelica, 15 parts of lithospermum, 6 parts of roasted liquorice, 15 parts of knotweed, 5 parts of trillium and 10 parts of lotus seed pods.

4. The method for preparing the Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The raw materials are soaked in water, then boiled and extracted, and the obtained water extract is obtained.

5. The preparation method according to claim 4, characterized in that: Decoction extraction twice, first boil over high heat, then simmer over low heat for 30-40 minutes, filter, and take the filtrate; add water to the obtained medicinal residue, boil over high heat, then simmer over low heat for 20-30 minutes, filter, and take the filtrate; combine the filtrates to obtain; and / or The soaking water temperature is 30-45°C and the soaking time is 20-40 minutes; and / or The preparation method further comprises concentrating and / or drying the obtained water extract.

6. The preparation method according to claim 5, characterized in that: Decoction extraction twice, first boil over high heat, then simmer over low heat for 35 minutes, filter, and take the filtrate; add water to the obtained medicinal residue, boil over high heat, then simmer over low heat for 25 minutes, filter, and take the filtrate; combine the filtrates to obtain; and / or The soaking time is 30 minutes.

7. Use of the traditional Chinese medicine composition according to any one of claims 1 to 3 or the traditional Chinese medicine composition prepared by the preparation method according to any one of claims 4 to 6 in the preparation of a drug for treating HPV infection after conization.

8. The use according to claim 7, characterized in that: The medicine is an oral preparation.

9. The use according to claim 8, characterized in that: The oral preparation also includes a pharmaceutically acceptable excipient.

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