Traditional Chinese medicine composition as well as preparation method and application thereof in preparation of external tincture

Abstract

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly discloses a traditional Chinese medicine composition as well as a preparation method and application thereof in preparation of external tincture, the traditional Chinese medicine composition comprises the following components in parts by weight: 20-40 parts of scutellaria baicalensis, 10-30 parts of angelica sinensis, 20-40 parts of rheum officinale, 20-40 parts of coptis chinensis, 10-30 parts of pseudobulbus cremastrae seu pleiones, 20-40 parts of golden cypress, 10-30 parts of pericarpium citri reticulatae and 10-30 parts of liquorice. The external tincture of the traditional Chinese medicine composition has excellent functions of stopping bleeding, diminishing inflammation, relieving pain, removing blood stasis, astringing and promoting tissue regeneration, has a good treatment effect on slight trauma, small wounds, pain and bleeding, mosquito bite and bee venom, and has the effects of counteracting toxic substances, removing stasis, diminishing swelling, expelling pus, healing sores, stopping bleeding, promoting wound healing and the like; the traditional Chinese medicine composition has a remarkable treatment effect on large and small deteriorated abscesses, finger and toe paronychia and other innominate pyogenic infections.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition, a preparation method thereof, and an application thereof in the preparation of an external tincture. Background Art

[0002] In modern medicine, external tinctures are widely used in the treatment of various skin diseases, traumas, inflammations and other diseases due to their advantages such as convenient use, relatively fast efficacy, and no side effects. Especially in the treatment of hemostasis, anti - inflammation, pain relief, removing stasis, etc., external tinctures are gradually favored by more and more people. With the characteristics of rapid penetration through the skin and direct action on the lesion site, external tinctures have become an important treatment means for treating traumas, skin diseases, muscle pains and other diseases.

[0003] However, most of the existing external tinctures use single chemical drugs or a certain type of natural drugs, with relatively single effects, and may stimulate the skin during drug use, and even produce certain side effects. Therefore, how to develop a new traditional Chinese medicine composition that can achieve multiple treatment effects such as hemostasis, anti - inflammation, pain relief, removing stasis, astringency, and promoting granulation through the synergistic effect of multiple drugs, and can be safely and effectively used in the preparation of external tinctures is a technical problem to be solved urgently. Summary of the Invention

[0004] The present invention provides a traditional Chinese medicine composition, a preparation method thereof, and an application thereof in the preparation of an external tincture. The traditional Chinese medicine composition tincture has excellent functions of hemostasis, anti - inflammation, pain relief, removing stasis, astringency, and promoting granulation.

[0005] The present invention solves its technical problems by adopting the following technical solutions: A traditional Chinese medicine composition comprising the following components in parts by weight: 20 - 40 parts of Scutellaria baicalensis Georgi, 10 - 30 parts of Angelica sinensis (Oliv.) Diels, 20 - 40 parts of Rheum palmatum L., 20 - 40 parts of Coptis chinensis Franch., 10 - 30 parts of Cremastra appendiculata (D. Don) Makino, 20 - 40 parts of Phellodendron amurense Rupr., 10 - 30 parts of Citrus reticulata Blanco, and 10 - 30 parts of Glycyrrhiza uralensis Fisch.

[0006] Scutellaria baicalensis Georgi: Scutellaria baicalensis Georgi can clear heat and detoxify, has a certain hemostatic effect on the control of inflammatory reactions and bleeding caused by damp - heat, and can enhance the anti - inflammatory effects of other drugs.

[0007] Angelica sinensis (Oliv.) Diels: Angelica sinensis (Oliv.) Diels can promote blood circulation to remove stasis, improve blood flow, and at the same time has the effect of enriching blood, which helps the hemostasis process.

[0008] Rheum palmatum L.: Rheum palmatum L. can clear heat and detoxify, remove the heat toxin in the body, dispel stasis, and has a certain pain - relieving effect. It can help clear the damp - heat in the body, promote blood circulation, and reduce inflammatory reactions.

[0009] Coptis chinensis Franch.: Coptis chinensis Franch. can further improve the inhibitory effect on inflammation and damp - heat, and enhance the hemostatic effect.

[0010] Cremastra appendiculata: Cremastra appendiculata helps to relieve the inflammatory reaction caused by tumors and nodules, and further enhances the effects of anti - inflammation, pain relief, and swelling reduction.

[0011] Phellodendron amurense: Phellodendron amurense can remove damp - heat toxins in the body, and together with other components, improve the treatment effect on damp - heat and toxins, and reduce inflammation.

[0012] Tangerine peel: Tangerine peel helps to regulate qi, harmonize the spleen and stomach, promote qi and blood circulation, and relieve the pain and discomfort caused by qi stagnation.

[0013] Licorice root: Licorice root can harmonize other drugs, reduce adverse reactions, enhance the drug effect, and at the same time helps with anti - inflammation, pain relief, and enhancing immunity.

[0014] The present invention creatively combines the above - mentioned raw medicinal materials in a specific ratio. Under the synergy of each component, a traditional Chinese medicine composition with excellent functions of hemostasis, anti - inflammation, pain relief, stasis removal, astringency, and granulation promotion is obtained. The tincture of the traditional Chinese medicine composition has a good therapeutic effect on minor traumatic wounds, bleeding from wounds and pains, mosquito bites, and bee stings. At the same time, it has the effects of attacking toxins and resolving masses, detumescence and discharging pus, astringing sores and stopping bleeding, and promoting wound healing, and has a significant therapeutic effect on large and small suppurating sores and paronychia of fingers and toes (commonly known as snake - head fingers) and other unknown toxic swellings.

[0015] The present invention creatively adopts the ingredients of strengthening the healthy qi and eliminating pathogenic factors, fully reflecting the treatment method of traditional medicine of eliminating pathogenic factors while strengthening the healthy qi. By improving the patient's own resistance and immunity, it provides a practical new drug for the efficient treatment of patients with various diseases such as minor traumatic wounds.

[0016] As a preferred embodiment of the present invention, it comprises the following components in parts by weight: Scutellaria baicalensis 30 parts, Angelica sinensis 20 parts, Rheum palmatum 30 parts, Coptis chinensis 30 parts, Cremastra appendiculata 20 parts, Phellodendron amurense 30 parts, Tangerine peel 20 parts, Licorice root 20 parts.

[0017] The present invention also provides an application of a traditional Chinese medicine composition in the preparation of an external tincture.

[0018] As a preferred embodiment of the present invention, the external tincture is an external tincture with drugs for promoting blood circulation to remove stasis, detumescence and pain relief and / or hemostasis.

[0019] As a preferred embodiment of the present invention, the preparation method of the external tincture comprises the following steps: Weigh each raw material medicine according to the ratio. Cut Scutellaria baicalensis, Angelica sinensis, Rheum palmatum, Coptis chinensis, Cremastra appendiculata, Phellodendron amurense, Citrus reticulata, and Glycyrrhiza uralensis into thin slices, put them into a sealed container together, add ethanol solution, cover it, soak, shake, stir, let it stand, and pour out the supernatant; add ethanol solution to the medicinal residues again, cover it, soak, shake, stir, let it stand, and pour out the supernatant; combine the supernatants, let it stand still, and filter to obtain the external tincture.

[0020] As a preferred embodiment of the present invention, the mass concentration of the ethanol solution is 50 - 75%.

[0021] As a preferred embodiment of the present invention, the solid-liquid ratio of the materials in the sealed container to the ethanol solution is 1 g : (2 - 10) mL.

[0022] As a preferred embodiment of the present invention, the solid-liquid ratio of the medicinal residues to the ethanol solution is 1 g : (2 - 10) mL.

[0023] As a preferred embodiment of the present invention, the standing time is 6 - 12 days.

[0024] As a preferred embodiment of the present invention, the standing still time is 12 - 36 h.

[0025] The beneficial effects of the present invention: The tincture of the traditional Chinese medicine composition of the present invention has excellent functions of hemostasis, anti-inflammatory, analgesic, removing stasis, astringing, and promoting granulation. The tincture of the traditional Chinese medicine composition has a good therapeutic effect on minor traumatic small wounds, bleeding from wounds and pains, mosquito bites, and bee stings. At the same time, it has the effects of attacking toxins and dissipating nodules, detumescence and discharging pus, astringing sores and stopping bleeding, and promoting wound healing, and has a significant therapeutic effect on large and small worsening boils and unknown swelling toxins such as paronychia of fingers and toes (commonly known as snake head fingers). Specific Embodiments

[0026] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0027] In the present invention, among the technically characterized described in an open-ended manner, a closed technical solution composed of the listed features is included, and an open technical solution of the listed features is also included.

[0028] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are considered continuous and include the minimum and maximum values of the range, as well as every value between such minimum and maximum values. Further, when the range refers to integers, every integer between the minimum and maximum values of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0029] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0030] The reagents or instruments used in the present invention that are not indicated by the manufacturer are all conventional products that can be obtained through commercial purchase; the raw materials used in each comparative example and the raw materials used in the parallel experiments of each example are the same commercially available products unless otherwise specified.

[0031] Example 1 An external tincture of a traditional Chinese medicine composition, comprising the following components in parts by weight: 30 parts of Scutellaria baicalensis, 20 parts of Angelica sinensis, 30 parts of Rheum palmatum, 30 parts of Coptis chinensis, 20 parts of Cremastra appendiculata, 30 parts of Phellodendron amurense, 20 parts of Citrus reticulata, 20 parts of Glycyrrhiza uralensis.

[0032] The preparation method of the external tincture of the traditional Chinese medicine composition comprises the following steps: Weigh each raw material according to the ratio, cut Scutellaria baicalensis, Angelica sinensis, Rheum palmatum, Coptis chinensis, Cremastra appendiculata, Phellodendron amurense, Citrus reticulata, and Glycyrrhiza uralensis into thin slices, put them together into a closed container, add a 60 wt% ethanol solution according to the ratio of 1 g: 5 mL, cover the container and soak, shake and stir, leave for 10 days, and pour out the supernatant; add a 60 wt% ethanol solution to the medicinal residues according to the solid-liquid ratio of 1 g: 5 mL, cover the container and soak, shake and stir, leave for 10 days, and pour out the supernatant; combine the supernatants, let stand for 24 h, filter, and obtain the external tincture of the traditional Chinese medicine composition.

[0033] Example 2 An external tincture of a traditional Chinese medicine composition, comprising the following components in parts by weight: 20 parts of Scutellaria baicalensis, 30 parts of Angelica sinensis, 20 parts of Rheum palmatum, 40 parts of Coptis chinensis, 10 parts of Cremastra appendiculata, 40 parts of Phellodendron amurense, 10 parts of Citrus reticulata, 30 parts of Glycyrrhiza uralensis.

[0034] The preparation method of the external tincture of the traditional Chinese medicine composition comprises the following steps: Weigh each raw material medicine according to the ratio, cut Scutellaria baicalensis, Angelica sinensis, Rheum palmatum, Coptis chinensis, Cremastra appendiculata, Phellodendron amurense, Citrus reticulata Blanco, and Glycyrrhiza uralensis Fisch. into thin slices, put them together into a sealed container, add 60wt% ethanol solution according to the ratio of 1g:5mL, cover it, soak, shake and stir, leave it for 10 days, and pour out the supernatant; add 60wt% ethanol solution to the medicinal residues according to the solid-liquid ratio of 1g:5mL, cover it, soak, shake and stir, leave it for 10 days, and pour out the supernatant; combine the supernatants, let it stand for 24h, filter, and obtain the external tincture of the traditional Chinese medicine composition.

[0035] Example 3 An external tincture of a traditional Chinese medicine composition, comprising the following components in parts by weight: 40 parts of Scutellaria baicalensis, 10 parts of Angelica sinensis, 40 parts of Rheum palmatum, 20 parts of Coptis chinensis, 30 parts of Cremastra appendiculata, 20 parts of Phellodendron amurense, 30 parts of Citrus reticulata Blanco, and 10 parts of Glycyrrhiza uralensis Fisch.

[0036] The preparation method of the external tincture of the traditional Chinese medicine composition comprises the following steps: Weigh each raw material medicine according to the ratio, cut Scutellaria baicalensis, Angelica sinensis, Rheum palmatum, Coptis chinensis, Cremastra appendiculata, Phellodendron amurense, Citrus reticulata Blanco, and Glycyrrhiza uralensis Fisch. into thin slices, put them together into a sealed container, add 60wt% ethanol solution according to the ratio of 1g:5mL, cover it, soak, shake and stir, leave it for 10 days, and pour out the supernatant; add 60wt% ethanol solution to the medicinal residues according to the solid-liquid ratio of 1g:5mL, cover it, soak, shake and stir, leave it for 10 days, and pour out the supernatant; combine the supernatants, let it stand for 24h, filter, and obtain the external tincture of the traditional Chinese medicine composition.

[0037] Comparative Example 1

[0038] The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not add Scutellaria baicalensis and Angelica sinensis, and the others are the same.

[0039] An external tincture of a traditional Chinese medicine composition, comprising the following components in parts by weight: 30 parts of Rheum palmatum, 30 parts of Coptis chinensis, 20 parts of Cremastra appendiculata, 30 parts of Phellodendron amurense, 20 parts of Citrus reticulata Blanco, and 20 parts of Glycyrrhiza uralensis Fisch.

[0040] Comparative Example 2

[0041] The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 does not add Rheum palmatum and Cremastra appendiculata, and the others are the same.

[0042] An external tincture of a traditional Chinese medicine composition, comprising the following components in parts by weight: 30 parts of Scutellaria baicalensis, 20 parts of Angelica sinensis, 30 parts of Coptis chinensis, 30 parts of Phellodendron amurense, 20 parts of Citrus reticulata Blanco, and 20 parts of Glycyrrhiza uralensis Fisch.

[0043] Comparative Example 3

[0044] The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not add Phellodendron amurense and Coptis chinensis, and the others are the same.

[0045] An external tincture of a traditional Chinese medicine composition, comprising the following components in parts by weight: 30 parts of Scutellaria baicalensis, 20 parts of Angelica sinensis, 30 parts of Rheum palmatum, 20 parts of Cremastra appendiculata, 20 parts of Citrus reticulata, 20 parts of Glycyrrhiza uralensis.

[0046] Comparative Example 4

[0047] The difference between Comparative Example 4 and Example 1 is that in Comparative Example 4, an equal amount of Panax notoginseng is used to replace Scutellaria baicalensis, and an equal amount of Angelica dahurica is used to replace Angelica sinensis, and the others are the same.

[0048] An external tincture of a traditional Chinese medicine composition, comprising the following components in parts by weight: 30 parts of Panax notoginseng, 20 parts of Angelica dahurica, 30 parts of Rheum palmatum, 30 parts of Coptis chinensis, 20 parts of Cremastra appendiculata, 30 parts of Phellodendron amurense, 20 parts of Citrus reticulata, 20 parts of Glycyrrhiza uralensis.

[0049] Comparative Example 5

[0050] The difference between Comparative Example 5 and Example 1 is that in Comparative Example 5, an equal amount of Poria cocos is used to replace Angelica sinensis, and an equal amount of Bletilla striata is used to replace Cremastra appendiculata, and the others are the same.

[0051] An external tincture of a traditional Chinese medicine composition, comprising the following components in parts by weight: 30 parts of Scutellaria baicalensis, 20 parts of Poria cocos, 30 parts of Rheum palmatum, 30 parts of Coptis chinensis, 20 parts of Bletilla striata, 30 parts of Phellodendron amurense, 20 parts of Citrus reticulata, 20 parts of Glycyrrhiza uralensis.

[0052] Test Example

[0053] 1. Establish a rabbit traumatic bleeding model: Select adult rabbits with an average body weight between 4 kg and 4.5 kg, and inflict a wound 4 cm long and 1.5 cm deep on the hind limbs of the adult rabbits to establish a bleeding model for the rabbits.

[0054] Select 120 successfully modeled adult rabbits, with 15 rabbits in each group, and apply the external tincture of the traditional Chinese medicine composition of the example and the control group respectively. After hemostasis, put the adult rabbits into the cage, and continuously apply the medicine (each plaster contains 2 g of the external tincture of the traditional Chinese medicine composition) for 3 days, once a day, and statistically analyze the wound healing area and calculate the wound healing rate.

[0055]

[0056] It can be seen from Table 1 that the external tincture of the traditional Chinese medicine composition of the present invention has excellent hemostatic, stasis-removing and astringent effects, can effectively promote wound healing and improve the wound healing rate.

[0057] 2. Experimental samples: Fix the limbs of 6-8 week-old SPF-grade ICR mice on a flat plate and shave their abdomens (ten mice in each group, using the external tincture of the traditional Chinese medicine composition of the examples and comparative examples respectively), and then apply the patch (each patch contains 2 g of the external tincture of the traditional Chinese medicine composition) on the abdomen of the mice. Within 10 minutes of observation, count the number of twists of the mice (abdomen contraction and concavity, hind limb extension, hip elevation, creeping), and record the average value of the number of twists. The test results are shown in Table 2.

[0058] Table 2

[0059] As can be seen from Table 2, the external tincture of the traditional Chinese medicine composition described in the present invention has excellent analgesic effects.

[0060] 3. HaCaT (human keratinocytes) are cultured in Medium154 medium containing 10% fetal bovine serum, 100 U / ml penicillin, and 100 μg / ml streptomycin at 37°C and 5% CO 2 under saturated humidity of 98%. Logarithmic growth phase cells are selected and inoculated into a 96-well culture plate. After the cells are completely adherent after 24 h of culture, the medium is replaced with Medium154 medium containing the external tincture of the traditional Chinese medicine composition (final concentration 0.12 mg / mL) (the negative control group is not replaced). After incubation for 12 h, irradiate with UVB at a dose of 25 mJ / cm 2 for 10 min. After irradiation, collect the cell culture supernatant, and detect the concentration of endothelin-1 in the supernatant using a human ELISA kit. Each experiment is repeated three times, and the average value is taken as the final result. The results are shown in Table 3.

[0061] Endothelin-1 inhibition rate (%) = (endothelin-1 expression level in the sample / endothelin-1 expression level in the negative control group) × 100%.

[0062] Table 3

[0063] As can be seen from Table 3, the external tincture of the traditional Chinese medicine composition described in the present invention has excellent anti-inflammatory effects.

[0064] In summary, the external tincture of the traditional Chinese medicine composition of the present invention has excellent functions of hemostasis, anti-inflammatory, analgesic, removing stasis, astringing, and promoting granulation. It has good therapeutic effects on minor traumatic wounds, bleeding from wounds and pains, mosquito bites, and bee stings. At the same time, it has the effects of attacking toxins and resolving masses, detumescence and discharging pus, astringing sores and stopping bleeding, and promoting wound healing. It has significant therapeutic effects on large and small suppurating sores and unnamed toxic swellings such as paronychia of fingers and toes.

[0065] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A Chinese medicine composition, characterized in that: The invention comprises the following components in parts by weight: 20-40 parts of scutellaria baicalensis, 10-30 parts of angelica sinensis, 20-40 parts of rhubarb, 20-40 parts of coptis chinensis, 10-30 parts of trillium, 20-40 parts of phellodendron chinense, 10-30 parts of tangerine peel and 10-30 parts of liquorice.

2. The Chinese medicine composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 30 parts of scutellaria baicalensis, 20 parts of angelica sinensis, 30 parts of rhubarb, 30 parts of coptis chinensis, 20 parts of trillium, 30 parts of phellodendron chinense, 20 parts of tangerine peel and 20 parts of liquorice.

3. Use of the Chinese medicine composition according to any one of claims 1 to 2 in the preparation of external tinctures.

4. The use according to claim 3, characterized in that: The external tincture is an external tincture with the effects of promoting blood circulation, removing blood stasis, reducing swelling, relieving pain and / or stopping bleeding.

5. The use according to claim 3, characterized in that: The preparation method of the external tincture comprises the following steps: Weigh each raw material medicine according to the ratio, cut scutellaria baicalensis, angelica sinensis, rhubarb, coptis chinensis, trillium, phellodendron, tangerine peel and liquorice into thin slices, put them into a sealed container together, add ethanol solution, cover the container, immerse, shake, stir, leave, and pour out the supernatant; add ethanol solution to the residue again, cover the container, immerse, shake, stir, leave, and pour out the supernatant; combine the supernatants, let stand, filter, and obtain a tincture for external use.

6. The use according to claim 5, characterized in that: The mass concentration of the ethanol solution is 50-75%.

7. The use according to claim 5, characterized in that: The solid-to-liquid ratio of the material in the sealed container to the ethanol solution is 1 g: (2-10) mL.

8. The use according to claim 5, characterized in that: The solid-liquid ratio of the medicinal residue to the ethanol solution is 1 g: (2-10) mL.

9. The use according to claim 5, characterized in that: The placement time is 6 to 12 days.

10. The use according to claim 5, characterized in that: The standing time is 12 to 36 hours.

Images