Production process of liquid edible mushroom beverage

Abstract

The invention relates to the technical field of drinks, and discloses a preparation process of a liquid edible fungus drink, which comprises the following steps: S1, preparing a PDA culture medium; s2, mother strain preparation; s3, preparing a shake flask strain culture medium; s4, fermentation tank operation treatment; s5, filling treatment is conducted, potatoes serve as the raw material of the PDA culture medium, and the potatoes are cleaned, peeled and weighed; the method comprises the following steps: slicing, shredding and dicing potatoes, weighing 1000ml of water by using a measuring cylinder, adding the water into a stainless steel pot, and starting to heat; according to the preparation process of the liquid edible mushroom beverage, edible mushrooms contain various nutritional ingredients such as amino acids, proteins, lipids, vitamins, mineral elements and nucleotides, so that the liquid edible mushroom beverage has the characteristics of high protein, low sugar, low fat and coexistence of various amino acids; and through further treatment of cellulase and papain, small molecular substances which are more easily absorbed and utilized by a human body are obtained.

Description

Technical Field

[0001] The present invention relates to the technical field of beverages, and particularly to a production process of a liquid edible mushroom beverage. Background Art

[0002] A liquid edible mushroom beverage is a beverage made by liquid culture and processing technologies of the effective components of edible mushrooms. With the continuous improvement of people's awareness of health and health care, the liquid edible mushroom beverage has broad development prospects.

[0003] In the prior art, the liquid edible mushroom beverage has relatively single components and only has simple functions of supplementing vitamins and proteins, resulting in poor functionality of the liquid edible mushroom beverage. In view of this, we have proposed a production process of a liquid edible mushroom beverage. Summary of the Invention

[0004] The purpose of the present invention is to provide a production process of a liquid edible mushroom beverage to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A production process of a liquid edible mushroom beverage, including the following steps:

[0006] S1. Preparation of PDA medium;

[0007] S2. Preparation of mother culture;

[0008] S3. Preparation of shake flask strain medium;

[0009] S4. Fermenter operation and treatment;

[0010] S5. Filling treatment;

[0011] The raw material of the PDA medium is potatoes. Wash the potatoes, peel them, and weigh them. The potatoes can be sliced, shredded, or diced. Measure 1000 ml of water with a measuring cylinder and add it to a stainless steel pot, then start heating. Add 200 grams of potatoes and heat until boiling, then turn to low heat to maintain a gentle boil until the potatoes are cooked but not mushy. Filter with 8 layers of gauze and take the filtrate and add it back to a clean pot. Make up the water to 1000 ml for the first time and continue heating, then add 20 grams of agar. Stir while heating until the agar is completely dissolved. Make up the water to 1000 ml for the second time, add the weighed other medicines, and heat with low heat until the medicines are completely dissolved. Subpackage: The subpackage amount for each test tube is 1 / 5 to 2 / 5 of the test tube length, or the subpackage amount can also be determined according to the required slope length;

[0012] For the preparation of the mother culture, select robust fruiting bodies that are seven to eight mature and exhibit the typical characteristics of the variety; cut off the mushroom stalk with a scalpel; squeeze out most of the alcohol from the alcohol cotton ball and then wipe the surface of the mushroom body; in a laminar flow hood, wipe the surface of the mushroom body in the same way; under aseptic operation, take a tissue block from the center of the mushroom cap to the center of the slant. The tissue block is a square with a side length of 2-3 mm, and then cultivate it.

[0013] The mother culture includes: Pleurotus ostreatus, Flammulina velutipes, Lentinula edodes, Pleurotus eryngii, Auricularia auricula-judae, Hericium erinaceus, Cordyceps militaris, Pleurotus citrinopileatus, Armillaria mellea, Ganoderma lucidum, Hypsizygus marmoreus, Hypsizygus tessellatus, Pleurotus pulmonarius, Morchella esculenta.

[0014] Optionally, S1 further includes: performing in a sterile room; placing a wooden strip with a thickness of 1-2 cm on the table and pressing heavy objects at both ends; placing the test tubes after drying the cotton plugs on the wooden strip, ensuring that the culture medium does not stick to the cotton plugs; covering the placed test tubes with a clean towel; it will solidify in about 12 hours; use it within 5 days and do not store it for a long time.

[0015] Optionally, S2 includes: observing the mother culture, detecting the growth trend and contamination situation every day, promptly picking out those with contamination and poor growth to avoid being covered by hyphae in a timely manner; when the hyphae grow to the point where the tip hyphae can be picked with an inoculation hook, pick the tip hyphae for tube transfer (tip detoxification), which can be done 1-3 times; gradually eliminate those with poor growth, and conduct a formal tube transfer confirmation for those that meet the requirements to confirm that they conform to the characteristics of the mother culture stage of the variety.

[0016] Optionally, S2 further includes:

[0017] S21. Wipe the inside of the laminar flow hood with a 75% alcohol cotton ball, place items such as an alcohol lamp, alcohol cotton, forceps, lighter, inoculation hook, etc., and wipe and disinfect them with alcohol.

[0018] S22. Close the glass window, turn on the fan and ultraviolet light.

[0019] S23. After 30 minutes, prepare for inoculation.

[0020] S24. Outside the laminar flow hood, wear gloves and wipe your hands and the mother culture with an alcohol cotton ball.

[0021] S25. Turn off the ultraviolet light, turn on the fluorescent lamp, and keep the fan on.

[0022] S26. Open the glass window, put your hands and the mother culture into the laminar flow hood, place the mother culture to one side, take out several alcohol cotton balls with forceps and place them on the laminar flow hood.

[0023] S27. Wipe your hands, the mother culture, the inoculation hook, and the tabletop of the operation area. Do not wipe other items again.

[0024] S28. Light the alcohol lamp.

[0025] S29. Pick up the inoculation hook with the right hand, heat the inoculation hook until it turns red with the outer flame, pick up the stock culture with the left hand, remove the cotton plug with the right hand under the protection of the flame, and place the cotton plug on the laminar flow hood;

[0026] S210. Hold the stock culture with the left hand, quickly pass the mouth of the test tube through the outer flame, burn the inoculation hook again with the right hand, then insert the inoculation hook into the stock culture test tube, and let the inoculation hook cool by sticking to the upper wall of the test tube;

[0027] S211. After the inoculation hook cools down, use the inoculation hook to remove the 1 - 2 cm part at the front end of the slant, remove the climbing hyphae and aerial hyphae with the inoculation hook, and then temporarily place the inoculation hook inside the test tube;

[0028] S212. Take a test tube with the right hand, place it parallel to the left hand and the stock culture test tube with the mouths of the test tubes aligned, with both mouths under the protection of the flame, remove the cotton plug with the right hand, and hold it with the little finger of the right hand;

[0029] S213. Cut a 3×3 mm piece of spawn with the inoculation hook and quickly place it in the center of the slant;

[0030] S214. Then, quickly put the inoculation hook back into the original stock culture test tube, pass the cotton plug through the flame, and plug it onto the test tube that was just inoculated under the protection of the flame;

[0031] S215. Put the inoculated test tube aside, pick up another one with the right hand at the same time, and continue the transfer until all inoculations are completed;

[0032] S216. Paste a label on the inoculated stock culture, indicating the variety, date, generation number, etc., and put it into the incubator;

[0033] S217. Clean the laminar flow hood, and wipe the laminar flow hood and all items with alcohol cotton;

[0034] S218. Turn on the ultraviolet lamp and the fan, and disinfect for at least 30 minutes;

[0035] Optionally, the culture temperatures of different stock cultures are as follows: Pleurotus ostreatus: 22 - 24°C; Flammulina velutipes: 19°C; Lentinula edodes: 24 - 25°C; Pleurotus eryngii: 22°C; Auricularia auricula: 24 - 25°C; Hericium erinaceus: 22°C; Cordyceps militaris: 18°C; Pleurotus citrinopileatus: 22°C; Armillaria mellea: 24 - 25°C; Ganoderma lucidum: 24 - 25°C; Hypsizygus marmoreus, Hypsizygus tessellatus, and Hypsizygus ulmarius: 22°C; Pleurotus pulmonarius: 22 - 24°C; Morchella esculenta: 15°C.

[0036] Optionally, the said S3 includes:

[0037] S31. Calculate the dosage of each raw material for 1 shake flask according to the formula;

[0038] S32. Weigh each raw material and drug separately;

[0039] S33. Add the weighed medicines into Erlenmeyer flasks respectively;

[0040] S34. Add 1 magnetic stir bar;

[0041] S35. Measure 600 ml of water with a measuring cylinder;

[0042] S36. First add about 200 ml into the Erlenmeyer flask, shake well, and then add the remaining water;

[0043] S37. Add 1 small drop of defoamer and shake well;

[0044] S38. Wipe the inner wall of the bottle mouth clean;

[0045] S39. Make a cotton plug or a silica gel plug and wrap it with a layer of kraft paper;

[0046] S310. Sterilize at 121 °C for 50 minutes;

[0047] S311. Dry the cotton plug for 10 minutes;

[0048] S312. Place it in the pre-prepared ultra-clean bench to cool;

[0049] S313. Inoculate in time after cooling.

[0050] Optionally, the shake flask strain inoculation treatment in S3 includes the following steps:

[0051] T1. Prepare the inoculation items;

[0052] T2. Wipe the inside of the ultra-clean bench and the items with alcohol cotton;

[0053] T3. Turn on the fan and ultraviolet light and keep for at least 30 minutes;

[0054] T4. After 30 minutes, outside the ultra-clean bench, put on sterile gloves and wipe the mother culture and both hands with alcohol cotton;

[0055] T5. Reach both hands into the ultra-clean bench;

[0056] T6. First take out several pieces of alcohol cotton from the wide-mouth bottle and set aside;

[0057] T7. Wipe both hands, the mother culture, the inoculation hook and the operating area tabletop with alcohol cotton;

[0058] T8. Light the alcohol lamp;

[0059] T9. Under the protection of the flame, pull out the cotton plug of the mother culture, and at the same time keep the test tube mouth under the protection of the flame;

[0060] T10. After disinfecting the inoculation hook with the flame, enter the test tube and cool it along the inner wall of the test tube;

[0061] T11. After the inoculation hook has cooled down, first remove the wall-climbing and aerial hyphae, and then cut off about 2 cm from the front end of the slant and discard it.

[0062] T12. Use the right hand to pull out the cotton plug of the Erlenmeyer flask and hold it with the little finger.

[0063] T13. Use the inoculation hook to pick 6 - 8 inoculation blocks of 3×3 mm and put them into the Erlenmeyer flask.

[0064] T14. Burn the cotton plug, cover it, and then cover it with kraft paper.

[0065] T15. Write down the inoculation date and variety.

[0066] Optionally, S4 includes the following steps:

[0067] S41. Wash the tank: Wash it on the same day it is used up.

[0068] S42. Inspect: Inspect the water circuit, electric circuit, air circuit, valves, pressure gauges, rubber gaskets, etc. The key point is the filter element, and inspect it every time.

[0069] S43. Add most of the water into the tank, fill the jacket with water, drain 5 liters, and start heating both the tank and the jacket simultaneously.

[0070] S44. Calculate the dosage of raw materials according to the formula, weigh them, add them into the stainless - steel bucket, add enough warm water into the stainless - steel bucket, and stir evenly until the white sugar is completely dissolved.

[0071] S45. When the fermenter is heated to 60 - 70 °C, add the mixed raw materials into the tank, and at the same time, introduce air, stir while heating, make up the water volume to the fixed - volume position, add defoamer, measure the pH, cover the lid, tighten the screws, and open the exhaust valve.

[0072] S46. Start sterilization at 122 °C for 70 minutes; during the sterilization process, drain the liquid 3 times for the "dead corners" in the same way; the filter is sterilized by introducing steam, and there is always a slight drainage at all the ends of the filter.

[0073] S47. Operations of important valves during the sterilization process.

[0074] Intake valve of the fermenter: Before 100 °C: Open; After 100 °C: Close.

[0075] Exhaust valve of the fermenter: Before 100 °C: Fully open; At 100 °C: Start to gradually decrease; At 122 °C: Slightly drain.

[0076] Steam outlet valve of the jacket: Before 100 °C: Fully open (to drain cold air); At 100 °C: Close; At 122 °C: Open, introduce it into the filter to sterilize the filter.

[0077] S48. The sterilization is completed, and the power supply is turned off;

[0078] S49. Slightly open the exhaust valve a little more;

[0079] S410. Close the steam introduced into the filter (close the valve starting from the end);

[0080] S4. Pass clean air into the filter to dry the filter element (important), for at least 10 minutes;

[0081] S411. Drain the steam in the jacket, introduce cold water into the jacket, and start cooling;

[0082] S412. The temperature and pressure start to drop. When the pressure in the fermenter drops to 0.02 - 0.05 Mpa, pass clean air into the fermenter, and the steam positive pressure changes to air positive pressure (positive pressure throughout the process);

[0083] S413. The temperature continues to drop. When the temperature drops to the inoculation temperature, close the cold water, and the cooling is completed.

[0084] S414. Prepare for inoculation.

[0085] Optionally, the S5 includes: filling the fermented liquid in S4 and storing the packaged products in the warehouse.

[0086] Compared with the prior art, the present invention provides a production process of a liquid edible mushroom drink, having the following beneficial effects:

[0087] The production process of this liquid edible mushroom drink, because the edible mushroom contains various nutritional components such as amino acids, proteins, polysaccharides, lipids, vitamins, mineral elements, nucleotides, etc., has the characteristics of high protein, low sugar, low fat, and coexistence of multiple amino acids, and obtains small molecule substances that are more easily absorbed and utilized by the human body through further treatment with cellulase and papain. Specific embodiments

[0088] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0089] The present invention provides a technical solution: a production process of a liquid edible mushroom drink, including the following steps:

[0090] S1. Preparation of PDA medium: Conducted in a sterile room. Place a wooden strip with a thickness of 1 - 2 cm on the table and press heavy objects at both ends. Arrange the test tubes with dried cotton plugs on the wooden strip, ensuring that the medium does not stick to the cotton plugs. Cover the arranged test tubes with a clean towel. It will solidify in about 12 hours and should be used within 5 days and not stored for a long time.

[0091] S2. Preparation of stock culture: Observe the stock culture, detect the growth trend and contamination situation every day, and promptly pick out those with contamination and poor growth to avoid being covered by hyphae in a timely manner. When the hyphae grow to the point where the tip hyphae can be picked with an inoculation hook, pick the tip hyphae for tube transfer (tip detoxification), which can be done 1 - 3 times. Gradually eliminate those with poor growth, and conduct formal tube transfer confirmation for those meeting the requirements to confirm that they conform to the characteristics of the stock culture stage of this variety.

[0092] S21. Wipe the inside of the laminar flow hood with 75% alcohol cotton balls, place items such as alcohol lamps, alcohol cotton, forceps, lighters, inoculation hooks, etc. inside, and disinfect them by wiping with alcohol.

[0093] S22. Close the glass window, turn on the blower and ultraviolet light.

[0094] S23. After 30 minutes, prepare for inoculation.

[0095] S24. Outside the laminar flow hood, wear gloves and wipe your hands and the stock culture with alcohol cotton.

[0096] S25. Turn off the ultraviolet light, turn on the fluorescent lamp, and keep the blower on.

[0097] S26. Open the glass window, put your hands and the stock culture into the laminar flow hood, place the stock culture aside, take out several pieces of alcohol cotton with forceps, and place them on the laminar flow hood.

[0098] S27. Wipe your hands, the stock culture, the inoculation hook, and the tabletop of the operation area. Other items do not need to be wiped again.

[0099] S28. Light the alcohol lamp.

[0100] S29. Pick up the inoculation hook with your right hand, heat the inoculation hook red with the outer flame, pick up the stock culture with your left hand, remove the cotton plug with your right hand under the protection of the flame, and place the cotton plug on the laminar flow hood.

[0101] S210. Hold the stock culture with your left hand, quickly burn the test tube mouth with the outer flame, burn the inoculation hook again with your right hand, then insert the inoculation hook into the stock culture test tube, and let the inoculation hook cool by sticking to the upper wall of the test tube.

[0102] S211. After the inoculation hook cools, remove the 1 - 2 cm part at the front end of the slant with the inoculation hook, remove the climbing hyphae and aerial hyphae with the inoculation hook, and then temporarily place the inoculation hook in the test tube.

[0103] S212. Take a test tube with your right hand and place it parallel to the mother culture test tube in your left hand, aligning the tube mouths, with both tube mouths under the protection of the flame. Pull out the cotton plug with your right hand and hold it between the little finger of your right hand.

[0104] S213. Cut a 3×3 mm piece of the strain with an inoculation hook and quickly place it in the center of the slant.

[0105] S214. Then, quickly put the inoculation hook back into the original mother culture test tube. Pass the cotton plug over the flame and, under the protection of the flame, plug it onto the test tube that was just inoculated.

[0106] S215. Place the inoculated test tube aside. Pick up another one with your right hand at the same time and continue the transfer until all inoculations are completed.

[0107] S216. Paste a label on the inoculated mother culture, indicating the variety, date, generation number, etc., and place it in the incubator.

[0108] S217. Clean the laminar flow hood and wipe the laminar flow hood and all items with alcohol cotton.

[0109] S218. Turn on the ultraviolet lamp and the blower and disinfect for at least 30 minutes.

[0110] S3. Preparation of the shake flask strain medium.

[0111] S31. Calculate the dosage of each raw material for 1 shake flask according to the formula.

[0112] S32. Weigh each raw material and drug separately.

[0113] S33. Add the weighed drugs into the Erlenmeyer flask respectively.

[0114] S34. Add 1 magnetic stir bar.

[0115] S35. Measure 600 ml of water with a measuring cylinder.

[0116] S36. First, add about 200 ml to the Erlenmeyer flask, shake well, and then add the remaining water.

[0117] S37. Add 1 small drop of antifoaming agent and shake well.

[0118] S38. Wipe the inner wall of the bottle mouth clean.

[0119] S39. Make a cotton plug or a silicone plug and wrap it with a layer of kraft paper.

[0120] S310. Sterilize at 121 °C for 50 minutes.

[0121] S311. Dry the cotton plug for 10 minutes.

[0122] S312. Place it in the pre-prepared laminar flow hood to cool.

[0123] S313. Inoculate in time after cooling.

[0124] Perform shake flask strain inoculation treatment, including the following steps:

[0125] T1. Prepare the inoculation items;

[0126] T2. Wipe the inside of the laminar flow hood and the items with alcohol cotton;

[0127] T3. Turn on the fan and ultraviolet light and keep for at least 30 minutes;

[0128] T4. After 30 minutes, outside the laminar flow hood, put on sterile gloves and wipe the mother culture and both hands with alcohol cotton;

[0129] T5. Reach both hands into the laminar flow hood;

[0130] T6. First take out a few pieces of alcohol cotton from the wide-mouth bottle for standby;

[0131] T7. Wipe both hands, the mother culture, the inoculation hook and the tabletop of the operation area with alcohol cotton;

[0132] T8. Light the alcohol lamp;

[0133] T9. Under the protection of the flame, pull out the cotton plug of the mother culture while keeping the test tube mouth under the protection of the flame;

[0134] T10. After disinfecting the inoculation hook with the flame, enter the test tube and cool it along the inner wall of the test tube;

[0135] T11. After the inoculation hook cools down, first remove the climbing and aerial hyphae, and then cut off about 2 cm at the front end of the inclined surface and remove it;

[0136] T12. Use the right hand to pull out the cotton plug of the Erlenmeyer flask and hold it with the little finger;

[0137] T13. Use the inoculation hook to pick 6 - 8 inoculation blocks of 3×3 mm into the Erlenmeyer flask;

[0138] T14. Burn the cotton plug, cover it, and cover it with kraft paper;

[0139] T15. Write down the inoculation date and variety.

[0140] S4. Fermenter operation treatment;

[0141] S4 includes the following steps:

[0142] S41. Wash the tank: Wash it on the same day when it is used up;

[0143] S42. Check: Check the water circuit, electric circuit, gas circuit, valves, pressure gauges, rubber gaskets, etc., with a focus on the filter element for each check;

[0144] S43. Add most of the water into the tank, fill the interlayer with water, drain 5 liters, and start heating the tank and the interlayer simultaneously;

[0145] S44. Calculate the dosage of raw materials according to the formula, weigh them, add them into a stainless steel barrel, add enough water (warm water) into the stainless steel barrel, and stir evenly until the white sugar is completely dissolved;

[0146] S45. When the fermentation tank is heated to 60 - 70 °C, add the mixed raw materials into the tank, and at the same time, introduce air, stir while heating, make up the water volume to the fixed volume position, add defoamer, measure the pH, cover the lid, tighten the screws, and open the exhaust valve;

[0147] S46. Start sterilization at 122 °C for 70 minutes; during the sterilization process, drain the liquid 3 times at the "dead corners" in the same way; the filter is sterilized by introducing steam, and all the ends of the filter are always slightly drained;

[0148] S47. Operations of important valves during the sterilization process;

[0149] Intake valve of the fermentation tank: Before 100 °C: Open; After 100 °C: Close;

[0150] Exhaust valve of the fermentation tank: Before 100 °C: Fully open; At 100 °C: Start to gradually decrease; At 122 °C: Slightly drain;

[0151] Steam outlet valve of the interlayer: Before 100 °C: Fully open (to drain cold air); At 100 °C: Close; At 122 °C: Open, introduce it into the filter to sterilize the filter;

[0152] S48. After sterilization, turn off the power supply;

[0153] S49. Slightly open the exhaust a little more;

[0154] S410. Close the steam introduced into the filter (close the valve starting from the end);

[0155] Introduce clean air into the S4 filter to dry the filter element (important), for at least 10 minutes;

[0156] S411. Drain all the steam in the interlayer, introduce cold water into the interlayer, and start cooling;

[0157] S412. The temperature and pressure start to drop. When the pressure in the fermentation tank drops to 0.02 - 0.05 Mpa, introduce clean air into the fermentation tank, and change the steam positive pressure to air positive pressure (positive pressure throughout the process);

[0158] S413. The temperature continues to drop. When the temperature drops to the inoculation temperature, close the cold water, and the cooling ends.

[0159] S414. Prepare for inoculation.

[0160] S5, Filling process: The fermented liquid in S4 is filled, and the packaged products are stored in the warehouse.

[0161] The raw material of the PDA medium is potatoes. Wash the potatoes, peel them, and weigh them. The potatoes can be sliced, shredded, or diced. Measure 1000 ml of water with a graduated cylinder and add it to a stainless steel pot, then start heating. Add 200 grams of potatoes and heat until boiling, then turn to low heat to maintain a gentle boil until the potatoes are cooked but not mushy. Filter with 8 layers of gauze and pour the filtrate back into a clean pot. Add water for the first time to make it up to 1000 ml and continue heating. Add 20 grams of agar. Stir while heating until the agar is completely dissolved. Add water for the second time to make it up to 1000 ml, add the other weighed drugs, and heat over low heat until the drugs are completely dissolved. Subpackage: The subpackage amount for each test tube is 1 / 5 to 2 / 5 of the test tube length, or the subpackage amount can also be determined according to the required slope length.

[0162] For the preparation of the mother culture, select healthy fruiting bodies that are seven or eight mature and have the typical characteristics of the variety. Cut off the mushroom stalk with a scalpel. Squeeze out most of the alcohol from the alcohol cotton and then wipe the surface of the mushroom body. In the ultra-clean bench, wipe the surface of the mushroom body in the same way. Under aseptic operation, take a tissue block from the center of the mushroom cap to the center of the slope. The tissue block is a 2-3 mm square, and then culture it.

[0163] The mother cultures include: Pleurotus ostreatus, Flammulina velutipes, Lentinula edodes, Pleurotus eryngii, Auricularia auricula, Hericium erinaceus, Cordyceps militaris, Pleurotus citrinopileatus, Armillaria mellea, Ganoderma lucidum, Hypsizygus marmoreus, Hypsizygus tessellatus, Pleurotus pulmonarius, Morchella esculenta.

[0164] The culture temperatures for different mother cultures are as follows: Pleurotus ostreatus: 22 - 24 °C; Flammulina velutipes: 19 °C; Lentinula edodes: 24 - 25 °C; Pleurotus eryngii: 22 °C; Auricularia auricula: 24 - 25 °C; Hericium erinaceus: 22 °C; Cordyceps militaris: 18 °C; Pleurotus citrinopileatus: 22 °C; Armillaria mellea: 24 - 25 °C; Ganoderma lucidum: 24 - 25 °C; Hypsizygus marmoreus, Hypsizygus tessellatus: 22 °C; Pleurotus pulmonarius: 22 - 24 °C; Morchella esculenta: 15 °C.

[0165] As an application of this embodiment: In the production process of this liquid edible mushroom drink, the edible mushrooms contain various nutrients such as amino acids, proteins, polysaccharides, lipids, vitamins, mineral elements, nucleotides, etc., and have the characteristics of high protein, low sugar, low fat, and coexistence of multiple amino acids. Through further treatment with cellulase and papain, small molecule substances that are more easily absorbed and utilized by the human body are obtained.

[0166] The above has generally described the present invention in detail, but based on the present invention, some modifications or improvements can be made, which are obvious to those of ordinary skill in the technical field. Therefore, modifications or improvements made without departing from the spirit of the present invention are within the protection scope of the present invention.

Claims

1. A process for producing a liquid edible fungus beverage, characterized in that: The steps include: S1, preparation of PDA medium; S2, preparation of mother stock; S3, preparation of shake flask culture medium; S4, fermentation tank operation and processing; S5, filling process; The raw material of the PDA culture medium is potatoes. The potatoes are washed, peeled, and weighed; the potatoes can be sliced, shredded, or diced. 1000 ml of water is weighed with a measuring cylinder, added to a stainless steel pot, and heating is started; 200 grams of potatoes are added, heated to boiling, and then the heat is reduced to maintain a slight simmer until the potatoes are cooked but not mushy; filtered with 8 layers of gauze, and the filtrate is added back to a clean pot; the water is added to 1000 ml for the first time, and the heating is continued, and 20 grams of agar is added; the agar is stirred while heating until the agar is completely dissolved; the water is added to 1000 ml for the second time, and other weighed drugs are added, and the drugs are heated on a low heat until the drugs are completely dissolved; Packaging: The packaging volume of each test tube is 1 / 5 to 2 / 5 of the length of the test tube. The packaging volume can also be determined according to the required inclined surface length; The mother seed preparation comprises selecting robust fruiting bodies with typical characteristics of the variety and being about 70% to 80% mature; cutting off the mushroom stem with a scalpel; squeezing out most of the alcohol from the alcohol cotton and then wiping the surface of the mushroom body; wiping the surface of the mushroom body in the same way in a super-cleaning environment; Aseptically take a tissue block from the center of the cap to the center of the bevel. The tissue block should be 2-3 mm square and then cultured. The mother species include: oyster mushroom, enoki mushroom, shiitake mushroom, king oyster mushroom, black fungus, velvet antler mushroom, cordyceps militaris, citronella mushroom, honey fungus, ganoderma lucidum, shiitake mushroom, white jade mushroom, seafood mushroom, pleurotus geesteranus, and morel.

2. The process for producing a liquid edible fungus beverage according to claim 1, characterized in that: The S1 further comprises: performing the process in a sterile room; placing a wooden bar with a thickness of 1-2 cm on the table, with weights pressed on both ends; placing the test tubes with dried cotton plugs on the wooden bar, and preventing the culture medium from sticking to the cotton plugs; covering the placed test tubes with a clean towel; solidifying in about 12 hours; using within 5 days and not storing for a long time.

3. The process for producing a liquid edible fungus beverage according to claim 1, characterized in that: The S2 includes: observing the mother strain, checking the growth and contamination conditions every day, picking out the contaminated and poorly growing strains in time to avoid being covered by mycelium due to delay; when the mycelium grows to the point where the tip can be picked up with an inoculation hook, picking up the tip and transferring it to a tube (detoxifying the tip), which can be done 1-3 times; gradually eliminating those with poor growth, and formally transferring those that meet the requirements to confirm that they meet the mother strain stage characteristics of the variety.

4. The process for producing a liquid edible fungus beverage according to claim 3, characterized in that: The S2 further comprises: S21. Wipe the ultra-clean interior with 75% alcohol cotton balls, put in alcohol lamps, alcohol cotton balls, tweezers, lighters, inoculation hooks and other items, and wipe with alcohol for disinfection; S22, close the glass windows, turn on the fan and ultraviolet light; S23, 30 minutes later, prepare for vaccination; S24. Outside the cleanroom, wear gloves and wipe your hands and mother stock with alcohol cotton; S25, turn off the ultraviolet light, turn on the fluorescent lamp, and keep the fan on; S26, open the glass window, put the mother seed into the clean room with both hands, put the mother seed aside, take out a few pieces of alcohol cotton with tweezers, and put them on the clean room; S27. Wipe your hands and the mother seed, inoculation hook, and the table in the operation area. Other items do not need to be wiped again; S28, light the alcohol lamp; S29. Pick up the inoculation hook with your right hand, burn it red with an external flame, pick up the mother seed with your left hand, pull out the cotton plug with your right hand under the protection of the flame, and place the cotton plug on the super-cleaner; S210, holding the mother seed in the left hand, quickly burn the mouth of the test tube with an external flame, and burn the inoculation hook again with the right hand, and then the inoculation hook enters the mother seed test tube, and the inoculation hook is attached to the upper wall of the test tube to cool; S211. After the inoculation hook is cooled, use the inoculation hook to remove the 1-2 cm portion of the front end of the bevel, use the inoculation hook to remove the wall-climbing hyphae and aerial hyphae, and then temporarily place the inoculation hook in a test tube; S212. Take a test tube with your right hand and place it in your left hand parallel to the mother test tube, with the test tube openings aligned and both under the protection of the flame. Remove the cotton plug with your right hand and hold it in your hand with your right little finger; S213, use the inoculation hook to cut a 3×3 mm bacterial seed block and quickly place it in the center of the slope; S214, then quickly put the inoculation hook back into the original mother seed test tube, pass the cotton plug over the flame, and then plug it into the test tube just inoculated under the protection of the flame; S215. Put the connected test tube aside, pick up another one with your right hand, and continue to transfer until all the inoculations are completed; S216. The inoculated mother stock is labeled with the variety, date, generation, etc., and placed in an incubator; S217, clean the super clean room, wipe the super clean room and all items with alcohol cotton; S218. Turn on the ultraviolet lamp and fan and disinfect for at least 30 minutes.

5. The process for producing a liquid edible fungus beverage according to claim 1, characterized in that: The cultivation temperatures of different mother strains are: Oyster mushroom: 22-24℃; Flammulina velutipes 19℃; Shiitake mushroom: 24-25℃; Pleurotus eryngii: 22℃; Auricularia auricula: 24-25℃; Pleurotus velutipes: 22℃; Cordyceps militaris: 18℃; Pleurotus citrinopileatus: 22℃; Honey fungus: 24-25℃; Ganoderma lucidum: 24-25℃; Oyster mushroom, Pleurotus ostreatus, and Pleurotus ostreatus: 22℃; Pleurotus geesteranus: 22-24℃; Morel: 15℃.

6. The process for producing a liquid edible fungus beverage according to claim 1, characterized in that: The S3 includes: S31, calculating the amount of various raw materials for one shake bottle according to the formula; S32. Weigh each raw material and drug separately; S33, adding the weighed drugs into the conical flasks respectively; S34, add 1 stirring bar; S35. Weigh 600 ml of water using a measuring cylinder; S36, first add about 200ml of the conical flask, shake well, and then add the remaining water; S37, add 1 small drop of defoamer and shake well; S38, clean the inner wall of the bottle mouth; S39, make a cotton plug or a silicone plug and wrap it with a layer of kraft paper; S310, sterilization: 121°C, 50 minutes; S311, drying the cotton plug for 10 minutes; S312, put it into the ultra-clean place prepared in advance to cool; S313. Vaccinate promptly after the temperature drops.

7. The process for producing a liquid edible fungus beverage according to claim 1, characterized in that: The shake flask bacterial strain inoculation process in S3 includes the following steps: T1. Prepare the vaccination items; T2. Wipe the ultra-clean interior and items with alcohol cotton; T3, turn on the fan and ultraviolet light for at least 30 minutes; T4. After 30 minutes, put on sterile gloves outside the clean room and wipe the mother seed and hands with alcohol cotton; T5, hands deep into the ultra-clean; T6. Take out a few pieces of alcohol cotton from the wide-mouth bottle and set aside; T7. Wipe your hands, mother seed, inoculation hook and the table in the operation area with alcohol cotton; T8. Light the alcohol lamp; T9. Under flame protection, remove the cotton plug of the mother seed and keep the mouth of the test tube under flame protection; T10. After flame disinfection, the inoculation hook is placed in the test tube and cooled against the inner wall of the test tube; T11. After the inoculation hook has cooled, first remove the wall-climbing and aerial hyphae, then cut off about 2 cm from the front end of the bevel and remove it; T12. Remove the cotton plug from the triangular bottle with your right hand and hold it in your hand with your little finger; T13, use the inoculation hook to take 6-8 pieces of 3×3 mm inoculation blocks into the conical flask; T14, burn the cotton plug, cover it, and cover it with brown paper; T15. Write down the vaccination date and type.

8. The process for producing a liquid edible fungus beverage according to claim 1, characterized in that: The S4 comprises the following steps: S41, tank washing: After using it on the same day, wash it on the same day; S42, Inspection: Check the water circuit, circuit, gas circuit, valve, pressure gauge, rubber pad, etc., with emphasis on the filter element, and check every time; S43, add most of the water into the tank, fill the interlayer with water, release 5 liters, and start heating the tank and the interlayer at the same time; S44. Calculate the amount of raw materials according to the formula, weigh them, add them into a stainless steel bucket, add enough water (warm water) into the stainless steel bucket, and stir evenly until the sugar is completely dissolved; S45. When the fermentation tank is heated to 60-70℃, add the mixed raw materials into the tank, introduce air at the same time, stir while heating, add water to the fixed volume, add defoamer, measure pH, cover the lid, tighten the screws, and open the exhaust valve; S46, start sterilization, 122℃, 70 minutes; the sterilization process is also "dead corner" drainage 3 times; The filter is steam sterilized, and all ends of the filter are always micro-drained; S47. Operation of important valves during sterilization; Fermentation tank air inlet valve: before 100℃: open; after 100℃: close; Fermentation tank exhaust valve: before 100℃: fully open; 100℃: start to gradually decrease; 122℃: slightly exhaust; Interlayer steam outlet valve: before 100℃: fully open (exhaust cold air); 100℃: closed; 122℃: open, pass through the filter to sterilize the filter; S48, sterilization is completed, turn off the power; S49, open the exhaust slightly wider; S410, close the steam entering the filter (close the valve from the end); Let clean air flow into the S4 filter to dry the filter element (important) for at least 10 minutes; S411, release the steam in the interlayer, pass cold water into the interlayer, and start cooling; S412, the temperature and pressure begin to drop. When the pressure of the fermentation tank drops to 0.02-0.05Mpa, clean air is introduced into the fermentation tank, and the steam positive pressure is converted into air positive pressure (full positive pressure); S413, the temperature continues to drop. When the temperature drops to the inoculation temperature, the cold water is turned off and the cooling is completed. S414, prepare for vaccination.

9. The process for producing a liquid edible fungus beverage according to claim 1, characterized in that: The step S5 includes: filling the fermented liquid in S4 and warehousing the packaged product.