Formula and preparation method of peptide-containing compound amino acid solid beverage

Abstract

The invention discloses a peptide-containing compound amino acid solid beverage formula and a preparation method. The solid beverage is prepared from the following raw materials in percentage by weight: 10 to 31 percent of compound amino acid, 8 to 16 percent of gamma-aminobutyric acid, 6 to 10 percent of Chinese yam peptide, 6 to 10 percent of ginseng peptide, 6 to 10 percent of sea cucumber peptide, 6 to 8 percent of yeast beta-glucan, 1 to 5 percent of taurine, 1 to 2 percent of rice germ powder, 0.5 percent of stevioside, 0.2 to 0.6 percent of inulin cellulose and 2 to 8 percent of epigallocatechin gallate (EGCG). The preparation method comprises extraction and purification of a mixture of Chinese yam, ginseng and sea cucumber peptide, extraction and purification of the additive and a freeze-drying technology of the solid beverage.

Description

Technical Field

[0001] The present application relates to the technical field of solid beverages, and in particular to a formula and a preparation method of a solid beverage containing a peptide-containing composite amino acid. Background Art

[0002] Solid beverages are solid products in granular, powdered or block form that are provided to consumers for drinking in the form of mixing or brewing. They are mainly made from food raw materials, food additives and other related auxiliary materials.

[0003] With the accelerated pace of modern life, the intensified social competition and the increased pressure of survival, various sub-health problems have also arisen.

[0004] Prior art CN 102524872 B discloses a ginseng peptide amino acid beverage, which solves the problem of relieving fatigue but cannot improve the digestion and absorption effect.

[0005] Therefore, solid beverages with certain health functions that can enhance the body's absorption effect when the digestion and absorption function is weak have come into being. Summary of the invention

[0006] The present application provides a formula and preparation method for a peptide-containing composite amino acid solid beverage. In order to solve the problems of energy loss after exercise and poor absorption due to weak stomach and intestines, the present application provides a solid beverage that can quickly provide energy and improve digestion and absorption. It uses composite amino acids, γ-aminobutyric acid, yeast β-glucan, taurine, and rice germ as raw materials, does not add various preservatives, and has scientifically proportioned various nutritional components, thereby solving the problems of rapid energy replenishment and improved absorption effect after exercise.

[0007] The first aspect of the present application provides a formula for a peptide-containing composite amino acid solid beverage, which consists of composite amino acids, γ-aminobutyric acid, yam peptides, ginseng peptides, sea cucumber peptides, yeast β-glucan, taurine, rice germ powder, steviol glycosides, inulin cellulose and additives.

[0008] Preferably, the formula of the peptide-containing composite amino acid solid beverage is as follows by weight percentage: composite amino acids 10-31%, γ-aminobutyric acid 8-16%, yam peptide 6-10%, ginseng peptide 6-10%, sea cucumber peptide 6-10%, yeast β-glucan 6-8%, taurine 1-5%, rice germ powder 1-2%, steviol glycoside 0.5%, inulin cellulose 0.2-0.6% and epigallocatechin gallate (EGCG) 2-8%.

[0009] Preferably, the complex amino acids are derived from soybeans.

[0010] Preferably, the additive is epigallocatechin gallate (EGCG), cyclodextrin and betaine, and their ratio is 1:1:1; the EGCG is derived from green tea leaves; the cyclodextrin is derived from corn starch; the betaine is derived from beet molasses.

[0011] Preferably, the compound amino acids include essential amino acids and non-essential amino acids. The essential amino acids include lysine, tryptophan, phenylalanine, methionine, threonine, leucine, isoleucine and valine; the non-essential amino acids include glycine, alanine, serine and glutamic acid.

[0012] Preferably, the mass ratio of the essential amino acids to the non-essential amino acids is 2:1; the mass ratio of lysine, tryptophan, phenylalanine, methionine, threonine, leucine, isoleucine and valine is 1:1:1:1:1:1:1:1; the mass ratio of glycine, alanine, serine and glutamic acid is 1:1:1:1.

[0013] On the other hand, the present application provides a method for preparing a peptide-containing compound amino acid solid beverage. Vacuum processing and mixing are adopted to reduce the contact between raw materials and air and reduce oxidation problems. Freeze-drying technology is used to solve the loss of nutritional components during the production process and retain the taste and nutrition of raw materials to the greatest extent.

[0014] Preferably, the method for preparing a peptide-containing compound amino acid solid beverage is as follows:

[0015] (1) Raw material treatment: Mix the compound amino acids, γ-aminobutyric acid, yeast β-glucan, taurine, and rice germ powder raw materials. For sufficient mixing, the raw materials must pass through a 100-mesh sieve to obtain a mixed raw material. Add deionized water with a volume 10 times that of the mixed raw material, set the rotation speed at 100 rpm / min, and stir and mix evenly at room temperature for 20 min to obtain a mixed stock solution.

[0016] (2) Preparation of peptide-containing mixed stock solution: Add the mixed peptide powder of yam, ginseng and sea cucumber and the additive extraction mixture to the mixed stock solution, set the rotation speed at 100 rpm / min, and stir and mix evenly at room temperature for 15 min to obtain a peptide-containing mixed stock solution.

[0017] (3) Flavoring: Add the stevioside and inulin cellulose to the peptide-containing mixed solution for flavoring, set the rotation speed at 120 rpm / min, and stir and mix evenly at room temperature for 30 min to obtain a peptide-containing compound amino acid beverage; Add the stevioside and inulin cellulose to the peptide-containing mixed solution for flavoring, set the rotation speed at 120 rpm / min, and stir and mix evenly at room temperature for 30 min to obtain a peptide-containing compound amino acid beverage.

[0018] (4) Pre-freezing: Place the peptide-containing compound amino acid beverage into the freezer, and rapidly freeze the material through a low-temperature refrigeration system at a temperature of -150°C for 3 hours to obtain the pre-frozen beverage;

[0019] (5) Drying: Place the pre-frozen beverage into the drying chamber of a freeze dryer. After evacuating to a certain extent, heat it through a heating plate to directly sublimate the ice crystals into water vapor for sublimation drying. Set the temperature at -10°C for 24 hours to control the moisture content below 10%. After the sublimation drying is completed, there is still a certain amount of bound water in the material, and then perform desorption drying at a temperature of 0°C for 4 hours to obtain the freeze-dried peptide-containing compound amino acid solid beverage;

[0020] (6) Packaging: Sub-pack and package the freeze-dried peptide-containing compound amino acid solid beverage to prevent it from absorbing moisture in the air and getting damp and deteriorating.

[0021] Preferably, the preparation method of the mixed peptide powder of yam, ginseng and sea cucumber is as follows:

[0022] (1) Pretreatment: Use the yam, ginseng and sea cucumber as raw materials. First, wash them to remove the soil and impurities on the surfaces of the yam, ginseng and sea cucumber, cut them into slices about 2 cm thick, and then pulverize them with a pulverizer to obtain a powdered mixture of yam, ginseng and sea cucumber;

[0023] (2) Mixed extraction: Extract the powdered mixture of yam, ginseng and sea cucumber. Add normal-temperature deionized water to the powdered mixture of yam, ginseng and sea cucumber at a mass-to-volume ratio of material:liquid = 1:10, and perform stirring extraction at an extraction temperature of 60°C, a rotation speed of 150 rpm / min for 2.5 hours to fully dissolve components such as proteins in water and obtain an extraction solution of the powdered mixture of yam, ginseng and sea cucumber;

[0024] (3) Enzymolysis: Filter the extraction solution to remove insoluble impurities to obtain a clarified extraction solution. Adjust its pH to 6.5 - 8.0, add protease for enzymolysis. The addition amount of protease is 2% of the protein mass of the powdered mixture of yam, ginseng and sea cucumber. The enzymolysis temperature is 50°C, the time is 7 hours, and the rotation speed is 100 rpm / min. At the end of the time, inactivate the protease by adjusting the temperature to 90°C for 10 minutes to obtain small-molecule mixed peptides of yam, ginseng and sea cucumber;

[0025] (4) Separation and purification: Separate and purify the mixed peptides of yam, ginseng and sea cucumber. Remove large particle impurities and unreacted substances through filtration and centrifugation to obtain a relatively pure solution of the mixed peptides of yam, ginseng and sea cucumber;

[0026] (5) Drying: The purified mixed peptide solution of Chinese yam, ginseng, and sea cucumber is dried by a drying technique at 50 °C to sublimate the excess moisture, obtaining a powder of the mixed peptide of Chinese yam, ginseng, and sea cucumber.

[0027] Preferably, the preparation method of the additive extraction mixture is as follows:

[0028] (1) EGCG pretreatment: Select green tea leaves rich in EGCG as raw materials, and crush them into a particle size of about 1 cm to facilitate full contact between the solvent and the tea leaves during the extraction process, obtaining EGCG raw materials;

[0029] (2) EGCG extraction: Mix the EGCG raw materials with deionized water at a mass-to-volume ratio of 1:10, with an extraction temperature of 45 °C and an extraction time of 2 h to obtain an EGCG extract; filter and centrifuge at 5000 rpm / min for 15 min to remove large particle impurities and unreacted substances, obtaining a pure EGCG extract;

[0030] (3) Cyclodextrin extraction: The cyclodextrin is derived from corn starch. Prepare a 10% starch milk, add an appropriate amount of cyclodextrin glucosyltransferase (CGT enzyme), with an enzyme addition amount of 20 U of enzyme per gram of starch, and carry out an enzymatic hydrolysis reaction at a temperature of 40 °C and a pH value between 6.0 and 8.0. The enzymatic hydrolysis time is 24 h, and after completion, the temperature is adjusted to 80 °C for 15 min to inactivate the enzyme, obtaining a cyclodextrin extract;

[0031] (4) Cyclodextrin separation and purification: Separate and purify the cyclodextrin extract, filter and centrifuge at 5000 rpm / min for 15 min to remove large particle impurities and unreacted substances, obtaining a pure cyclodextrin extract;

[0032] (5) Betaine extraction: The betaine is derived from beet molasses. Mix the beet molasses with ethanol at a mass-to-volume ratio of 1:10, with an extraction temperature of 50 °C, a rotation speed of 200 rpm / min, and an extraction time of 2 h to obtain a crude betaine extract;

[0033] (6) Betaine distillation: Filter the crude betaine extract and centrifuge at 000 rpm / min for 20 min to remove residues, and then distill the extract to remove the ethanol solvent, obtaining a betaine extract;

[0034] (7) Preparation of mixed crystals: Mix the EGCG extract, cyclodextrin extract, and betaine extract in a ratio of 1:1:1, and then refine the mixture to remove impurities and salts. Slowly heat the mixed solution to 70 °C to completely dissolve it into a saturated solution. Place the saturated solution in a cooling device and cool it at a cooling rate of 4 °C / h. When the temperature drops to 20 °C, crystals will gradually precipitate. Separate the crystals and the mother liquor by filtration to obtain a mixed crystal of EGCG, cyclodextrin, and betaine. Wash the crystals with deionized water and dry them at a low temperature to obtain an edible mixed crystal of EGCG, cyclodextrin, and betaine.

[0035] The beneficial effects of the embodiments of this application are as follows:

[0036] (1) This application provides a formula and preparation method for a peptide-containing compound amino acid solid beverage. The raw materials of compound amino acids, γ-aminobutyric acid, yeast β-glucan, taurine, and rice germ powder can provide energy for the body. When the body lacks carbohydrates and fats to provide energy, amino acids can, through deamination, convert the nitrogen-containing part into urea and excrete it from the body, while the nitrogen-free part can be oxidized and decomposed into carbon dioxide and water, releasing energy at the same time; γ-aminobutyric acid can lower blood pressure, regulate the opening of ion channels, reduce the excitability of nerve cells, thus playing a role in sedation and calming the nerves, helping to relieve anxiety and tension, and improving sleep quality; yeast β-glucan can stimulate the body's immune system, activate immune cells such as macrophages and natural killer cells, thereby enhancing the body's resistance. It can also act as a prebiotic to promote the growth and reproduction of beneficial intestinal bacteria, maintain the balance of the intestinal microecosystem, help improve intestinal digestion and absorption functions, prevent intestinal problems such as constipation and diarrhea, and can also bind to cholesterol, reducing the absorption of cholesterol in the intestine, and thus helping to lower the cholesterol level in the blood, which is beneficial to cardiovascular health; taurine has a certain blood pressure-lowering and blood lipid-lowering effect; rice germ powder is rich in various nutrients such as protein, vitamins (such as vitamin E, B vitamins, etc.), minerals (such as iron, zinc, magnesium, etc.), and dietary fiber, and can provide comprehensive nutritional support for the human body to meet the body's needs for various nutrients.

[0037] (2) Secondly, add yam, ginseng, and sea cucumber peptides. Yam peptides can strengthen the spleen and benefit the stomach, regulate gastrointestinal function, promote digestion and absorption, and have a certain improvement effect on symptoms such as weak spleen and stomach and indigestion; ginseng peptides can enhance the body's endurance and anti-fatigue ability, and improve the body's resistance to various harmful stimuli; sea cucumber peptides are rich in various nutrients such as protein, polysaccharides, and sea cucumber saponins. Sea cucumber peptides retain these nutrients, are easily absorbed by the human body, can provide rich nutrition for the body, help enhance physical fitness, improve the body's immune function, enhance the body's resistance, promote the body's metabolism, accelerate the excretion of fatigue substances, improve the body's endurance and anti-fatigue ability, and enable people to recover physical strength faster after exercise or work.

[0038] (3) Then add the mixed crystal of EGCG, cyclodextrin, and betaine. EGCG is a polyphenol with antioxidant activity, but it is easily oxidized and degraded in the external environment and has poor stability. Cyclodextrin has a unique cyclic molecular structure and can include EGCG in its cavity to form an inclusion compound. This inclusion effect can reduce the contact between EGCG and the external environment, reduce the possibility of its oxidation, and thus improve the stability of EGCG. Betaine has good water solubility and moisture retention, and it can play a role in regulating water activity in the system, reducing the degradation reaction of EGCG caused by water, and working together with cyclodextrin to improve the stability of EGCG in the product from different angles.

[0039] (4) Finally, add stevioside and inulin cellulose for flavoring. Stevioside meets people's demand for sweetness while reducing calorie intake, and is suitable for diabetics, obese people, etc. Inulin cellulose can promote intestinal peristalsis, reduce the occurrence of constipation, and help maintain the normal function of the intestine. Description of the Drawings

[0040] Figure 1 It is the extraction process flow chart of the mixed peptides of yam peptides, ginseng peptides, and sea cucumber peptides of the present invention;

[0041] Figure 2 It is the extraction and preparation process flow chart of the mixed crystal of EGCG, cyclodextrin, and betaine of the present invention;

[0042] Figure 3 It is the preparation process flow chart of the finished product of the present invention;

[0043] Figure 4 It is the bar chart of the average postprandial blood glucose change rate of the examples and the control group of the present invention. Detailed Embodiments

[0044] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0045] For a better understanding of the present invention rather than limiting its scope, all numbers representing amounts, percentages, and other numerical values used in this application should be understood to be modified by the word "about" in all cases. Each numerical parameter should be regarded as obtained at least according to the reported significant figures and by the conventional rounding method.

[0046] All components used in the present invention can be obtained by commercial purchase.

[0047] The present invention will be further illustrated by the following embodiments, but the embodiments do not limit the present invention in any way.

[0048] To achieve the first above-mentioned purpose, the present application proposes a formula for a peptide-containing compound amino acid solid beverage by weight percentage: compound amino acid 10-31%, γ-aminobutyric acid 8-16%, yam peptide 6-10%, ginseng peptide 6-10%, sea cucumber peptide 6-10%, yeast β-glucan 6-8%, taurine 1-5%, rice germ powder 1-2%, stevioside 0.5%, inulin cellulose 0.2-0.6%, and epigallocatechin gallate (EGCG) 2-8%.

[0049] Example 1

[0050] The peptide-containing compound amino acid solid beverage of the present invention is made of raw materials in the following weight percentage ranges: compound amino acid 28%, γ-aminobutyric acid 8%, yam peptide 7%, ginseng peptide 7%, sea cucumber peptide 7%, yeast β-glucan 6%, taurine 2%, rice germ powder 1%, stevioside 0.5%, inulin cellulose 0.4%, and epigallocatechin gallate (EGCG) 2%, and the rest is deionized water.

[0051] The preparation process of the above-mentioned mixed peptides of yam, ginseng, and sea cucumber is as Figure 1 shown, and the method steps are:

[0052] (1) Pretreatment: Using the yam, ginseng, and sea cucumber as raw materials, first wash them to remove the soil impurities on the surface of the yam, ginseng, and sea cucumber, cut them into slices about 2 cm thick, and pulverize them with a pulverizer to obtain a powdery mixture of yam, ginseng, and sea cucumber;

[0053] (2) Mixed extraction: Extract the powder mixture of yam, ginseng and sea cucumber. Add room temperature deionized water to the powder mixture of yam, ginseng and sea cucumber, with the mass-to-volume ratio of material: liquid being 1:10. Stir and extract at an extraction temperature of 60 °C, a rotation speed of 150 rpm / min, and a time of 2.5 h to fully dissolve components such as proteins in water, obtaining an extract of the powder mixture of yam, ginseng and sea cucumber;

[0054] (3) Enzymatic hydrolysis: Filter the extract to remove insoluble impurities, obtaining a clarified extract. Adjust its pH to 6.5 - 8.0, add protease for enzymatic hydrolysis. The addition amount of protease is 2% of the protein mass of the powder mixture of yam, ginseng and sea cucumber. The enzymatic hydrolysis temperature is 50 °C, the time is 7 h, and the rotation speed is 100 rpm / min. Inactivate the protease at the end of the time by adjusting the temperature to 90 °C for 10 min, obtaining a small molecule mixed peptide of yam, ginseng and sea cucumber;

[0055] (4) Separation and purification: Separate and purify the mixed peptide of yam, ginseng and sea cucumber. Remove large particle impurities and unreacted substances by filtration and centrifugation to obtain a relatively pure solution of the mixed peptide of yam, ginseng and sea cucumber;

[0056] (5) Drying: Dry the purified solution of the mixed peptide of yam, ginseng and sea cucumber using a drying technology at 50 °C to sublimate excess water, obtaining a powder of the mixed peptide of yam, ginseng and sea cucumber.

[0057] The above preparation process of the additive is as Figure 2 shown, and its method steps are:

[0058] (1) EGCG pretreatment: Select green tea leaves rich in EGCG as raw materials, and crush them into a particle size of about 1 cm to facilitate full contact between the solvent and the tea leaves during the extraction process, obtaining EGCG raw materials;

[0059] (2) EGCG extraction: Mix the EGCG raw materials with deionized water at a mass-to-volume ratio of 1:10, extract at an extraction temperature of 45 °C for 2 h to obtain an EGCG extract; Remove large particle impurities and unreacted substances by filtration and centrifugation at 5000 rpm / min for 15 min to obtain a pure EGCG extract;

[0060] (3) Cyclodextrin extraction: The cyclodextrin is derived from corn starch. Prepare a 10% starch milk, add an appropriate amount of cyclodextrin glucosyltransferase (CGT enzyme), with the addition amount of the enzyme being 20 U per gram of starch, and carry out an enzymatic reaction at a temperature of 40 °C and a pH value between 6.0 - 8.0. The enzymatic reaction time is 24 h, and after the end, adjust the temperature to 80 °C for 15 min to inactivate the enzyme to obtain a cyclodextrin extract;

[0061] (4) Cyclodextrin separation and purification: The cyclodextrin extract is separated and purified. By filtration and centrifugation at 5000 rpm / min for 15 min, large particle impurities and unreacted substances are removed to obtain a pure cyclodextrin extract;

[0062] (5) Betaine extraction: The betaine is derived from beet molasses. The beet molasses and ethanol are mixed at a mass - to - volume ratio of 1:10. The extraction temperature is 50 °C, the rotation speed is 200 rpm / min, and the extraction is carried out for 2 h to obtain a crude betaine extract;

[0063] (6) Betaine distillation: The crude betaine extract is filtered and centrifuged at 5000 rpm / min for 20 min to remove residues. Then the extract is distilled to remove the ethanol solvent to obtain a betaine extract;

[0064] (7) Preparation of mixed crystals: The EGCG extract, cyclodextrin extract, and betaine extract are mixed in a ratio of 1:1:1 and then refined to remove impurities and salts. The mixed solution is slowly heated to 70 °C to completely dissolve it into a saturated solution. The saturated solution is placed in a cooling device and cooled at a rate of 4 °C / h. When the temperature drops to 20 °C, crystals gradually precipitate. The crystals and the mother liquor are separated by filtration to obtain a mixed crystal of EGCG, cyclodextrin, and betaine. The crystals are washed with deionized water and dried at low temperature to obtain an edible mixed crystal of EGCG, cyclodextrin, and betaine.

[0065] Example 2

[0066] The solid beverage containing peptide - complex amino acids of the present invention is made from raw materials in the following weight percentage ranges: complex amino acids 28%, γ - aminobutyric acid 8%, yam peptide 7%, yeast β - glucan 6%, taurine 2%, rice germ powder 1%, stevioside 0.5%, inulin cellulose 0.4%, and epigallocatechin gallate (EGCG) 2%, and the rest is deionized water.

[0067] The above - mentioned preparation method of yam peptide is as follows:

[0068] (1) Pretreatment: Using the yam as the raw material, first wash it to remove the soil impurities on the surface of the yam, cut it into slices about 2 cm thick, and pulverize it with a pulverizer to obtain a yam powder;

[0069] (2) Extraction: The yam powder is extracted. Add normal - temperature deionized water, and the mass - to - volume ratio of material to liquid is 1:10. Stir and extract at an extraction temperature of 60 °C, a rotation speed of 150 rpm / min, and a time of 2.5 h to fully dissolve components such as proteins in water to obtain a yam extract;

[0070] (3) Enzymatic hydrolysis: Filter the extraction solution to remove insoluble impurities, obtaining a clarified extraction solution. Adjust its pH to 6.5 - 8.0, add protease for enzymatic hydrolysis. The addition amount of protease is 2% of the mass of yam protein. The enzymatic hydrolysis temperature is 50°C, the time is 7h, and the rotation speed is 100rpm / min. Inactivate the protease at the end of the time by adjusting the temperature to 90°C for 10min to obtain small molecule yam peptides.

[0071] (4) Separation and purification: Separate and purify the yam peptides, remove large particle impurities and unreacted substances by filtration and centrifugation to obtain a relatively pure yam peptide solution.

[0072] (5) Drying: Dry the purified yam peptide solution using a drying technology at 50°C to sublimate the excess water and obtain yam peptide powder.

[0073] The preparation method of the above additives is the same as that of the additives in Example 1.

[0074] Example 3

[0075] The peptide-containing compound amino acid solid beverage of the present invention is made of raw materials in the following weight percentage ranges: compound amino acids 28%, γ-aminobutyric acid 8%, yam peptides 7%, ginseng peptides 7%, sea cucumber peptides 7%, yeast β-glucan 6%, taurine 2%, rice germ powder 1%, stevioside 0.5%, inulin cellulose 0.4%, and the rest is deionized water.

[0076] The preparation method of the above mixed peptides of yam, ginseng and sea cucumber is the same as that of the mixed peptides of yam, ginseng and sea cucumber in Example 1.

[0077] Example 4

[0078] The peptide-containing compound amino acid solid beverage of the present invention is made of raw materials in the following weight percentage ranges: compound amino acids 28%, γ-aminobutyric acid 8%, yam peptides 7%, ginseng peptides 7%, sea cucumber peptides 7%, yeast β-glucan 6%, taurine 2%, rice germ powder 1%, stevioside 0.5%, inulin cellulose 0.4% and EGCG, and the rest is deionized water.

[0079] The preparation method of the above mixed peptides of yam, ginseng and sea cucumber is the same as that of the mixed peptides of yam, ginseng and sea cucumber in Example 1.

[0080] The preparation method of the above additives is as follows:

[0081] (1) EGCG pretreatment: Select green tea leaves rich in EGCG as raw materials, crush them into a particle size of about 1 cm to facilitate the full contact of the solvent with the tea leaves during the extraction process, and obtain EGCG raw materials.

[0082] (2) EGCG extraction: Mix the EGCG raw material with deionized water at a mass-to-volume ratio of 1:10. The extraction temperature is 45 °C, and the extraction is carried out for 2 h to obtain an EGCG extract. Through filtration and centrifugation at 5000 rpm / min for 15 min, large particle impurities and unreacted substances are removed to obtain a pure EGCG extract.

[0083] The difference between Example 1 and Example 2 is that Example 1 is a mixed peptide of Chinese yam, ginseng, and sea cucumber, while Example 2 is a Chinese yam peptide;

[0084] The difference between Example 1 and Example 3 is that Example 1 has an additive, while Example 3 does not have an additive;

[0085] The difference between Example 1 and Example 4 is that the additive in Example 1 is a mixture of EGCG, cyclodextrin, and betaine, while the additive in Example 4 is only EGCG;

[0086] The control group is deionized water.

[0087] Test Example 1: Animal digestion and absorption test - blood glucose monitoring

[0088] Experimental materials: The peptide-containing compound amino acid beverage of the present invention, deionized water;

[0089] Experimental animals: 50 SPF-grade SD rats were purchased from the Guangdong Provincial Medical Experimental Animal Center, with half males and half females, weighing between 200 - 250 g, 8 weeks old, 10 rats in each group, with half males and half females.

[0090] Test scheme:

[0091] Before the experiment, the rats were raised in a standard animal room with the temperature controlled at 22 ± 3 °C, humidity 40% - 60%, 12 h light / dark cycle, free access to water and food, and allowed to adapt to the environment for 3 - 5 d. 12 h before the start of the experiment, the rats were fasted but allowed to drink water, and the fasting body weight and the body weight before each day's experiment were recorded;

[0092] The peptide-containing amino acid beverage was concentrated 10-fold according to the adult recommended dose (50 ml / 60 Kg person) and prepared into a solution for gavage according to the rat body weight. The control group was given the same volume of deionized water, and the experiment was carried out for 30 d; Feed was provided at 9:00 and 15:00 every day. Blood was collected from the tip of the tail before feeding to record the blood glucose; After feeding, gavage was carried out, and blood was collected from the tip of the tail 30 min and 2 h after the end of gavage to record the blood glucose values, and the average blood glucose of each group was calculated.

[0093] The experimental results of the blood glucose values are shown in Table 1:

[0094] Table 1: Average blood glucose and average body weight gain and growth status of rats in 30 d

[0095]

[0096] The calculation formula for the blood glucose change rate is: Blood glucose change rate = (post-meal blood glucose value - pre-meal blood glucose value) / pre-meal blood glucose value × 100%, and the blood glucose change rate is as Figure 4 shown;

[0097] As shown in the above table, the pre-meal and post-meal blood glucose change rates in Example 1 are higher than those in Example 2, Example 3, Example 4 and the control group, and the post-meal blood glucose increase values are higher than those in Example 2, Example 3, Example 4 and the control group; the average weight gain in Example 1 is higher than that in Example 2, Example 3, Example 4 and the control group. Therefore, the digestion and absorption effect of Example 1 is higher than that of Example 2, Example 3, Example 4 and the control group.

[0098] Test Example 2: Animal toxicology experiment

[0099] The results of the acute toxicity test of the ginseng peptide amino acid beverage of the present invention are MTD > 25.0 g / kg·bw (equivalent to 700 times the recommended daily dose for humans). According to the acute toxicity classification, this product belongs to the non-toxic class. The results of the micronucleus test of rat bone marrow cells, the sperm aberration test of mice, and the Ames test are all negative. After 30 days of feeding test: Rats were fed for 30 days at 25, 50, and 100 times the recommended human dose. During the test period, the animals in each group grew and moved normally. At the end of the experiment, there was no significant difference in the body weight, weight gain, food intake, food utilization rate, and organ-body ratio of the animals in each dose group of the sample compared with the control group. There were significant differences in the blood biochemical and blood routine indexes of some dose groups of the sample compared with the control group, but they were all within the normal range and had no biological significance. There was no significant difference in the blood routine and blood biochemical indexes of the animals in other dose groups compared with the control group. Histopathological examination of the tested organs showed no specific lesions.

[0100] To achieve the above second object, the present application also proposes a preparation method of a peptide-containing compound amino acid solid beverage. The preparation process of the finished product is as Figure 3 shown, and its method steps are:

[0101] (1) Raw material treatment: Mix the compound amino acid, γ-aminobutyric acid, yeast β-glucan, taurine, and rice germ powder raw materials. For sufficient mixing, the raw materials must pass through a 100-mesh sieve to obtain a mixed raw material. Add deionized water with a volume 10 times that of the mixed raw material, set the rotation speed at 100 rpm / min, and stir and mix evenly at room temperature for 20 minutes to obtain a mixed stock solution;

[0102] (2) Preparation of peptide-containing mixed stock solution: Add the mixed powder of yam, ginseng, and sea cucumber peptides and the additive extraction mixture to the mixed stock solution, set the rotation speed at 100 rpm / min, and stir and mix evenly at room temperature for 15 minutes to obtain a peptide-containing mixed stock solution;

[0103] (3) Flavoring: Add the steviol glycoside and inulin cellulose to the peptide-containing mixed solution for flavoring. Set the rotation speed at 120 rpm / min and stir evenly at room temperature for 30 min to obtain a peptide-containing compound amino acid beverage; Add the steviol glycoside and inulin cellulose to the peptide-containing mixed solution for flavoring. Set the rotation speed at 120 rpm / min and stir evenly at room temperature for 30 min to obtain a peptide-containing compound amino acid beverage;

[0104] (4) Pre-freezing: Place the peptide-containing compound amino acid beverage in a freezer and quickly freeze the material through a low-temperature refrigeration system at a temperature of 150 °C for 3 h to obtain a pre-frozen beverage;

[0105] (5) Drying: Place the pre-frozen beverage in the drying chamber of a freeze dryer. After evacuating to a certain extent, heat it with a heating plate to directly sublimate the ice crystals into water vapor for sublimation drying. Set the temperature at -10 °C for 24 h to control the moisture content below 10%; After the sublimation drying is completed, there is still a certain amount of bound water in the material, and desorption drying is carried out at a temperature of 0 °C for 4 h to obtain a freeze-dried peptide-containing compound amino acid solid beverage;

[0106] (6) Packaging: Subpackage and package the freeze-dried peptide-containing compound amino acid solid beverage to prevent it from absorbing moisture in the air and getting damp and deteriorated.

[0107] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. All equivalent changes and improvements made within the scope of the application of the present invention shall still fall within the scope covered by the patent of the present invention.

Claims

1. A formula for a peptide-containing composite amino acid solid beverage, characterized in that: The solid beverage is prepared from raw materials in the following weight percentage range: 10-31% of complex amino acids, 8-16% of gamma-aminobutyric acid, 6-10% of yam peptide, 6-10% of ginseng peptide, 6-10% of sea cucumber peptide, 6-8% of yeast beta-glucan, 1-5% of taurine, 1-2% of rice germ powder, 0.5% of steviol glycoside, 0.2-0.6% of inulin cellulose and 2-8% of epigallocatechin gallate (EGCG).

2. A peptide-containing composite amino acid solid beverage formula as claimed in claim 1, characterized in that: The complex amino acids are derived from soybeans.

3. A peptide-containing composite amino acid solid beverage formula as claimed in claim 1, characterized in that: The additives are epigallocatechin gallate (EGCG), cyclodextrin and betaine, and the ratio thereof is 1:1:

1.

4. A peptide-containing composite amino acid solid beverage formula as claimed in claim 1, characterized in that: The complex amino acids include essential amino acids and non-essential amino acids. The essential amino acids include lysine, tryptophan, phenylalanine, methionine, threonine, leucine, isoleucine and valine; and the non-essential amino acids include glycine, alanine, serine and glutamic acid.

5. A peptide-containing composite amino acid solid beverage formula as described in claim 4, characterized in that the mass ratio of the essential amino acids to the non-essential amino acids is 2:1; the mass ratio of lysine, tryptophan, phenylalanine, methionine, threonine, leucine, isoleucine and valine is 1:1:1:1:1:1:1:1:1; the mass ratio of glycine, alanine, serine and glutamate is 1:1:1:1:

1.

6. A peptide-containing composite amino acid solid beverage formula as claimed in claim 1, characterized in that: The yam peptide, ginseng peptide and sea cucumber peptide are prepared from yam, ginseng and sea cucumber respectively through purification processes.

7. A peptide-containing composite amino acid solid beverage formula as described in any one of claims 1 to 6, wherein the solid beverage is used for energy supplementation after exercise and for people with weak gastrointestinal absorption.

8. A method for preparing a peptide-containing composite amino acid solid beverage according to any one of claims 1 to 7, characterized in that: The preparation method comprises: (1) Raw material processing: the composite amino acid, γ-aminobutyric acid, yeast β-glucan, taurine, and rice germ powder are mixed to obtain a mixed raw material, deionized water in an amount of 10 times the volume of the mixed raw material is added, the rotation speed is set to 100 rpm / min, and the mixture is stirred at room temperature for 20 minutes to obtain a mixed stock solution; (2) Preparation of peptide-containing mixed stock solution: Add the mixed peptide powder of yam, ginseng and sea cucumber and the additive extraction mixture into the mixed stock solution, set the speed to 100 rpm / min, and stir and mix at room temperature for 15 minutes to obtain the peptide-containing mixed stock solution; (3) Seasoning: adding the stevioside and inulin cellulose to the peptide-containing mixed solution for seasoning, setting the rotation speed to 120 rpm / min, stirring and mixing at room temperature for 30 min to obtain a peptide-containing composite amino acid beverage; (4) pre-freezing: pre-freezing the peptide-containing composite amino acid beverage to obtain a pre-frozen peptide-containing composite amino acid solid beverage; (5) Drying: placing the pre-frozen beverage into a drying chamber of a freeze dryer for drying to obtain a freeze-dried peptide-containing composite amino acid solid beverage; (6) Packaging: The freeze-dried peptide-containing composite amino acid solid beverage is divided and packaged to prevent it from absorbing moisture in the air and being damp and deteriorating.

9. The method for preparing a peptide-containing composite amino acid solid beverage according to claim 8, characterized in that: The extraction process of the mixed peptide powder of yam, ginseng and sea cucumber is as follows: (1) Pretreatment: The Chinese yam, ginseng and sea cucumber are used as raw materials, first washed to remove dirt and impurities on the surface of the Chinese yam, ginseng and sea cucumber, cut into slices with a thickness of about 2 cm, and crushed by a grinder to obtain a powdered mixture of the Chinese yam, ginseng and sea cucumber; (2) Mixed extraction: extracting the powdered mixture of yam, ginseng and sea cucumber, adding deionized water at room temperature to the powdered mixture of yam, ginseng and sea cucumber, with a mass volume ratio of material: liquid = 1:10, stirring and extracting, the extraction temperature is 60°C, the rotation speed is 150 rpm / min, and the time is 2.5 hours, so that the protein and other components are fully dissolved in the water, and obtaining an extract of the powdered mixture of yam, ginseng and sea cucumber; (3) Enzymolysis: The extract is filtered to remove insoluble impurities to obtain a clarified extract, the pH of which is adjusted to 6.5-8.0, and protease is added for enzymolysis, the amount of protease added is 2% of the protein mass of the yam, ginseng and sea cucumber powder mixture, the enzymolysis temperature is 50°C, the time is 7h, and the speed is 100rpm / min; at the end of the time, the protease is inactivated, the temperature is adjusted to 90°C, and the time is 10min to obtain a small molecule yam, ginseng and sea cucumber mixed peptide; (4) separation and purification: separating and purifying the mixed peptides of Chinese yam, ginseng and sea cucumber, removing large particles of impurities and unreacted substances by filtering and centrifuging, and obtaining a relatively pure mixed peptide solution of Chinese yam, ginseng and sea cucumber; (5) Drying: The purified yam, ginseng and sea cucumber mixed peptide solution is subjected to a drying technique at 50° C. to sublimate excess water to obtain yam, ginseng and sea cucumber mixed peptide powder.

10. The method for preparing a peptide-containing composite amino acid solid beverage according to claim 8, characterized in that: The additive extraction mixture is epigallocatechin gallate (EGCG), cyclodextrin and betaine, wherein the preparation method of the additive extraction mixture is: (1) EGCG pretreatment: EGCG-rich green tea leaves were selected as raw materials and crushed into particles of about 1 cm in size so that the solvent and the tea leaves could fully contact during the extraction process to obtain EGCG raw materials; (2) EGCG extraction: The EGCG raw material was mixed with deionized water in a mass volume ratio of 1:10, the extraction temperature was 45° C., and the extraction was performed for 2 h to obtain an EGCG extract; large particle impurities and unreacted substances were removed by filtration and centrifugation at 5000 rpm / min for 15 min to obtain a pure EGCG extract; (3) Cyclodextrin extraction: The cyclodextrin is derived from corn starch. The starch is prepared into 10% starch milk, and an appropriate amount of cyclodextrin glucosyltransferase (CGT enzyme) is added. The amount of enzyme added is 20U per gram of starch. The enzymatic hydrolysis reaction is carried out at a temperature of 40°C and a pH value between 6.0 and 8.

0. After the enzymatic hydrolysis time is 24 hours, the temperature is adjusted to 80°C, and the enzyme is inactivated for 15 minutes to obtain a cyclodextrin extract; (4) Separation and purification of cyclodextrin: The cyclodextrin extract is separated and purified by filtering and centrifuging at 5000 rpm / min for 15 min to remove large particle impurities and unreacted substances to obtain a pure cyclodextrin extract; (5) betaine extraction: the betaine is derived from beet molasses. The beet molasses is mixed with ethanol in a mass volume ratio of 1:10, the extraction temperature is 50° C., the rotation speed is 200 rpm / min, and the extraction is performed for 2 h to obtain a betaine extraction crude liquid; (6) betaine distillation: filtering the betaine extract crude solution, centrifuging at 5000 rpm / min for 20 min to remove the residue, and then distilling the extract to remove the ethanol solvent to obtain a betaine extract solution; (7) Preparation of mixed crystals: The EGCG extract, cyclodextrin extract and betaine extract are mixed in a ratio of 1:1:1 and then refined to remove impurities and salt therein. The mixed solution is slowly heated to 70°C to completely dissolve it to form a saturated solution. The saturated solution is placed in a cooling device and cooled at a cooling rate of 4°C / h. When the temperature drops to 20°C, crystals will gradually precipitate. The crystals and the mother liquor are separated by filtration to obtain EGCG, cyclodextrin and betaine mixed crystals. The crystals are washed with deionized water and dried at low temperature to obtain edible EGCG, cyclodextrin and betaine mixed crystals.

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