Application of phycocyanin or fermentation product of phycocyanin in preparation of skin care product and skin care product

Abstract

The invention discloses application of phycocyanin in preparation of a skin care product and the skin care product, and belongs to the technical field of cosmetics. The invention researches the application of the phycocyanin and the fermentation product thereof in the skin care direction, and provides the application of the phycocyanin or the fermentation product thereof in the preparation of a skin care product. The phycocyanin takes polyaspartic acid as a main chain and arginine as a side chain, and the molar ratio of the aspartic acid to the arginine is 1: (0.9-1.1); the skin care products include products that help improve skin moisture conditions and / or resist aging. The invention finds that the cystin with specific molecular weight can promote the vitality of keratinocytes, improve the water content of an epidermal structure and have anti-aging and moisturizing effects, the cystin leavening can improve the concentration of spermidine on the skin and play an anti-aging effect, and the cystin is a novel anti-aging skin care mode.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to the application of cyanophycin or its fermented product in the preparation of skin care products and a skin care product. Background Art

[0002] Disclosing the information of this background art section is only intended to enhance the understanding of the overall background of the present invention, and is not necessarily to be regarded as an admission or any form of implication that this information constitutes the prior art already known to those of ordinary skill in the art.

[0003] Cyanophycin is a class of amino acid polymers with polyaspartic acid as the main chain and basic amino acids or their derivatives as side chains, belonging to the category of biopolymers. In terms of molecular structure, cyanophycin is composed of aspartic acid forming an amide bond through the α-position amino group and carboxyl group to form the main chain, and the β-position carboxyl group forms an amide bond with the α-position amino group of basic amino acids, and basic amino acids form the side chain. At physiological pH, the carboxyl group and basic group on the basic amino acid molecule have positive and negative charges respectively, making cyanophycin an amphoteric ion polymer in structure. Due to the presence of opposite charges within the molecule to form internal salts, cyanophycin with a relatively large molecular weight has very poor water solubility and is only soluble in acidic or alkaline aqueous solutions. Reducing the molecular weight can improve water solubility. The diversity of the types and proportions of side chain amino acids of naturally sourced cyanophycin is very high.

[0004] Cyanophycin is generally produced by cyanophyceae algae (also known as cyanobacteria), especially species from the genera Spirulina, Nostoc, Scytonema, Synechococcus, Synechocystis, and Anabaena are commonly reported in research. Under optimized culture conditions, cyanophycin can reach more than 20% of the dry weight of the algal body. Some bacteria can also produce cyanophycin, such as strains from the genera Acinetobacteria and Bordetella. The cyanophycin synthase gene from cyanobacteria can also be expressed in models such as Escherichia coli for the production of cyanophycin.

[0005] In recent years, due to the development of green chemistry, environmentally friendly bioactive materials have been widely studied in the field of cosmetics. Among them, polyaspartic acid is a biocompatible and environmentally friendly polymer, an ionic polypeptide containing carboxylic acid side chains, and has become a highly potential alternative in the field of cosmetics. Patent CN113321808A discloses a multifunctional polyaspartic acid derivative. By coupling the polyaspartic acid derivative with L-lysine and coordinating with an alkyl chain, an L-lysine-polyaspartic acid derivative with amphiphilic molecules is formed, which synergistically increases skin permeability and promotes collagen production. Cyanophycin is also a polyaspartic acid derivative. It has been reported as a biodegradable biomaterial and a nanoparticle encapsulation material, but has not been applied to the field of skin care. Summary of the Invention

[0006] To solve the deficiencies of the prior art, the purpose of the present invention is to provide the application of cyanophycin or its ferment in the preparation of skin care products and a skin care product. The inventors have studied cyanophycin and its application in the field of skin care and found that specific cyanophycin can promote the viability of keratinocytes, increase the water content of the epidermal structure, and has anti-aging and moisturizing effects. The cyanophycin ferment can increase the concentration of spermidine on the skin and exert an anti-aging effect.

[0007] To achieve the above purpose, the technical solution of the present invention is as follows:

[0008] In the first aspect of the present invention, there is provided the application of cyanophycin or its ferment in the preparation of skin care products;

[0009] The cyanophycin has a polyaspartic acid main chain and an arginine side chain, and the molar ratio of aspartic acid to arginine is 1:0.9 - 1.1;

[0010] The skin care products include products that help improve the skin moisture condition and / or have anti-aging effects.

[0011] Cyanophycin has high diversity in its side chain composition. After analyzing the side chain components from the perspectives of skin characteristics and the amino acid metabolism pathway of skin flora, the inventors found that cyanophycin with arginine as the main component in the side chain and a molar ratio of aspartic acid to arginine close to 1:1 in the molecule has better skin care value.

[0012] It should be noted that the cyanophycin can be obtained from cyanophycin-producing algal species or strains, or can be purchased. The present invention does not limit the source of cyanophycin, nor the biological species of the cyanophycin source, as long as the cyanophycin structure meets the above requirements.

[0013] In some embodiments of the present invention, the molecular weight of the cyanophycin is less than or equal to 20 kD. Cyanophycin with a relatively large molecular weight has poor water solubility and is not convenient for use. While cyanophycin with a molecular weight less than or equal to 20 kD has good water solubility.

[0014] In some embodiments of the present invention, the molecular weight of the cyanophycin fermentate is 100 Da - 200 kDa, and more preferably 100 Da - 20 kDa.

[0015] In some embodiments of the present invention, the cyanophycin fermentate is a fermentate obtained by fermenting cyanophycin using a Lactobacillus with protease activity. Preferably, the protease includes any one or more of extracellular protease and cell wall protease.

[0016] The inventors found through research that adding a water-soluble cyanophycin fermentate within a specific molecular weight range, obtained by fermenting cyanophycin using a Lactobacillus with protease activity, to an in vitro culture of human skin flora can promote the production of spermidine, increase the concentration of spermidine in the skin, and exert an anti-aging effect.

[0017] It should be noted that there are various Lactobacilli with protease activity, such as Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus rhamnosus, and Lactobacillus bulgaricus, etc. The present invention does not limit the type of Lactobacillus, and other strains with protease activity can also be used.

[0018] In some embodiments of the present invention, the preparation method of the cyanophycin fermentate specifically includes the following steps:

[0019] Add cyanophycin to a medium containing sugars, inoculate Lactobacillus into the medium, culture at 30 - 50 °C for 4 - 48 h, and control the pH to be 4.0 - 7.0 during the culture; after the culture is completed, raise the temperature to inactivate Lactobacillus, centrifuge, and filter to obtain the cyanophycin fermentate.

[0020] Preferably, the content of cyanophycin in the medium is 1 - 5% w / w.

[0021] Preferably, the content of sugars in the medium is 0.1 - 2.0% w / w.

[0022] Preferably, the saccharide is a monosaccharide or a disaccharide; more preferably, the saccharide is one of glucose or sucrose.

[0023] Preferably, the concentration of Lactobacillus in the medium is 1E6 - 1E9 CFU / mL, preferably 1E8 - 1E9 CFU / mL.

[0024] Preferably, the culture temperature is 35 - 45 °C and the culture time is 12 - 24 h.

[0025] Preferably, the inactivation is to raise the temperature to 70 - 80 °C and keep warm for 10 - 20 min.

[0026] Preferably, the filtration is to use a membrane with a specific cut-off molecular weight to collect components within a specific molecular weight range, and the molecular weight range is 100 Da - 200 kDa, preferably 100 Da - 20 kDa.

[0027] In some embodiments of the present invention, in the cyanophycin, the molar ratio of aspartic acid to arginine is 1:0.95 - 1.05. Cyanophycin with an aspartic acid:arginine molar ratio close to 1:1 in the molecule has better skin care value.

[0028] In some embodiments of the present invention, the skin care product includes at least one of lotion, emulsion, essence, serum, mask, sunscreen, cream, eye cream, hand cream or body cream.

[0029] Preferably, the mask is a patch mask or a smear mask.

[0030] Preferably, the essence is essence water, essence oil or essence gel.

[0031] In some embodiments of the present invention, the content of cyanophycin in the skin care product is 0.01 - 5.0 wt%, preferably 0.1 - 1.0 wt%.

[0032] In some embodiments of the present invention, the improvement of skin moisture condition includes increasing the water content of the skin.

[0033] In some embodiments of the present invention, the anti-aging includes increasing the viability of keratinocytes or increasing the content of spermidine on the skin surface.

[0034] Spermidine is a multifunctional bioactive substance and is generally considered to have anti-aging effects through multiple mechanisms. By applying the cyanophycin ferment to the skin, the concentration of spermidine on the skin can be increased, and the anti-aging effect can be exerted, which is a novel anti-aging skin care method.

[0035] The second aspect of the present invention provides a skin care product containing 0.01 - 5.0 wt% of phycocyanin or its ferment, wherein the phycocyanin has a polyaspartic acid main chain and an arginine side chain, and the molecular weight is less than or equal to 20 kD; the ferment of the phycocyanin is a ferment obtained by fermenting phycocyanin with a protease-active Lactobacillus, and the molecular weight is 100 Da - 200 kDa.

[0036] In some embodiments of the present invention, the skin care product includes at least one of lotion, emulsion, essence lotion, essence, facial mask, sunscreen, cream, eye cream, hand cream or body cream.

[0037] Preferably, the facial mask is a patch facial mask or a smear facial mask.

[0038] Preferably, the essence is essence water, essence oil or essence gel.

[0039] In some embodiments of the present invention, the skin care product further includes cosmetically acceptable excipients.

[0040] Preferably, the excipients are one or more of solvents, thickeners, humectants, film-forming agents, liposomes, preservatives, colorants, pH regulators and fragrances.

[0041] Preferably, the solvent is water or an alcohol solvent.

[0042] The beneficial effects of the present invention are as follows:

[0043] The present invention studies the application of phycocyanin in skin care, and finds that phycocyanin has high diversity in side chain composition. After analyzing the side chain components of phycocyanin from the perspectives of skin characteristics and the amino acid metabolism pathway of skin flora, it is found that phycocyanin with an arginine-based side chain and a molar ratio of aspartic acid to arginine in the molecule close to 1:1 has better skin care value. It can promote the vitality of keratinocytes, increase the water content of the epidermal structure, and has anti-aging and moisturizing effects.

[0044] The present invention also studies the application of the ferment of phycocyanin in skin care, and finds that adding a water-soluble ferment of phycocyanin with a specific molecular weight range obtained by fermenting phycocyanin with a protease-active Lactobacillus strain to an in vitro culture of human skin flora can promote the production of spermidine. This ferment can be used in skin care products under the name of "Lactobacillus / phycocyanin extract fermentation product". Spermidine is a multifunctional bioactive substance and is generally considered to have anti-aging effects through multiple mechanisms. By applying a precursor substance - the ferment of phycocyanin that can promote the production of spermidine to skin flora, increasing the concentration of spermidine on the skin and exerting anti-aging effects is a novel anti-aging skin care method. Description of the Drawings

[0045] The accompanying drawings of the specification, which form a part of the present invention, are used to provide a further understanding of the present invention. The schematic embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention.

[0046] Figure 1 The effects of the water-soluble cyanophycin prepared in Example 1 of the present invention on skin cells at different concentrations. Among them, A is a picture of skin cells in the solvent control group after incubation, B is a picture of skin cells in the 0.078 mg / mL cyanophycin sample group after incubation, C is a picture of skin cells in the 0.156 mg / mL cyanophycin sample group after incubation, D is a picture of skin cells in the 0.315 mg / mL cyanophycin sample group after incubation, E is a picture of skin cells in the 0.625 mg / mL cyanophycin sample group after incubation, F is a picture of skin cells in the 1.250 mg / mL cyanophycin sample group after incubation, G is a picture of skin cells in the 2.500 mg / mL cyanophycin sample group after incubation, H is a picture of skin cells in the 5.000 mg / mL cyanophycin sample group after incubation, and I is a picture of skin cells in the 10.000 mg / mL cyanophycin sample group after incubation. Detailed implementation manners

[0047] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below with specific embodiments.

[0048] Example 1: Screening of cyanophycin-producing cyanobacteria (cyanobacteria) and preparation of cyanophycin

[0049] The collected soil samples, fresh river water samples, seawater samples, etc. are serially diluted with a pH 6.0, 0.05 N phosphate buffer solution, spread on BG11 solid medium plates and cultured. The culture conditions are 25 °C, 1000 lux white light source, 14 h - 10 h light cycle. After culturing for 1 - 2 weeks, green colonies are picked and streaked for purification to obtain pure single colonies, which are then inoculated into BG11 liquid medium for standby culture.

[0050] Take 10 mL of the liquid culture of the isolated cyanobacterial strain, centrifuge to collect the cells, add a little liquid nitrogen and grind to break the cells. After the liquid nitrogen evaporates, add 1 mL of 0.1 N hydrochloric acid solution, shake and then centrifuge to take the supernatant. Use the Sakaguchi reaction to measure the arginine content in the supernatant on a spectrophotometer. The algal species with a high arginine content can be confirmed as the cyanophycin-producing strain. The data of some strains are shown in Table 1. Strain CP1 is selected for further study. According to 16S rDNA sequencing, strain CP1 is identified as Anabaena variabilis.

[0051] Table 1 Arginine content after treatment with different algal cells

[0052]

[0053] For the amplification fermentation of cyanophycin by A. variabilis CP1, on the BG11 medium supplemented with precursor amino acids, after 120 h of cultivation, the cell yield was 10 g / L of cell dry weight. After cell disruption, the extract was leached with hydrochloric acid solution, and the pH of the leaching solution was adjusted to neutral to precipitate cyanophycin. After drying, the crude product was obtained with a yield of 2.2 - 2.5 g / L.

[0054] Example 2: Preparation of water-soluble cyanophycin

[0055] The cyanophycin obtained in Example 1 had poor water solubility due to its large molecular weight, which was not convenient for use. It was dissolved in a citric acid buffer solution with a pH of 3.0 at a concentration of 1.0% w / w, and ultrasonic cleavage treatment was carried out for 30 minutes. Samples were taken every 5 minutes and the pH was adjusted to ~7.0 to observe whether there was precipitation to judge the water solubility of the product. For the water-soluble product at pH ~7.0, SDS-PAGE was used to measure the molecular weight distribution. The results showed that the molecular weight of the water-soluble cyanophycin was mainly distributed below 20 kD. After adjusting the pH of the water-soluble cyanophycin solution to ~7.0, it was vacuum concentrated to about three times the concentration. Three volumes of absolute ethanol were added to each volume of the concentrated solution to precipitate cyanophycin. After filtration to obtain the solid precipitate, it was washed several times with 95% ethanol and then vacuum freeze-dried to obtain a low-molecular-weight cyanophycin product with a white powder appearance, with a molecular weight ≤20 kD. Amino acid composition analysis showed that the molar ratio of aspartic acid to arginine was 1:1.04.

[0056] Application Example 1: Effect of cyanophycin on skin cells

[0057] For the water-soluble cyanophycin prepared in Example 2, its effect on human keratinocytes was tested. The cryopreserved cells were inoculated into a culture flask for cultivation after resuscitation. When the plating rate reached about 60%, the cells were inoculated into a 96-well plate and incubated overnight in a CO 2 incubator (37 °C, 5% CO 2 ). The cells were divided into a zero-adjustment group, a solvent control group, and a sample group. The sample group had 8 concentration gradients of water-soluble cyanophycin, as shown in the test concentration table (Table 2), and 3 replicate wells were set at each concentration gradient. Different concentrations of sample working solutions were prepared according to the test concentration table (Table 2). When the plating rate of the cells in the 96-well plate reached 50% - 60%, drug administration was carried out. The solvent control group added 200 μL of culture medium to each well; the sample group added 200 μL of culture medium containing the corresponding concentration of the sample to be tested to each well; the zero-adjustment group had no cell inoculation and only added 200 μL of cell culture medium. After drug administration, the 96-well plate was placed in a CO 2 incubator (37 °C, 5% CO 2Cultivate for 24 h. After 24 h of cell incubation, discard the supernatant, add MTT working solution (0.5 mg / mL), incubate at 37 °C in the dark for 4 h. After incubation, discard the supernatant, add 150 μL of DMSO to each well, and read the OD value at 490 nm. The relative cell viability is calculated according to the following formula:

[0058] Relative cell viability (%) = (OD of sample well - OD of blank well) / (OD of solvent control well - OD of blank well) × 100%.

[0059] Cell photos were taken after discarding the supernatant under an inverted microscope after incubation.

[0060] Cell viability tests showed that at concentrations of 0.078 - 10 mg / mL, water-soluble phycocyanin could improve the viability of keratinocytes, and the optimal concentration in the test was 5 mg / mL. As an amino acid polymer, water-soluble phycocyanin can release amino acids as nutrients under the action of cell proteases and peptidases. Its zwitterionic nature will not cause pH and ion concentration imbalance, and it has high safety and efficacy.

[0061] Table 2 Test concentrations and cell viability detection results

[0062]

[0063] Cell photos are shown in Figure 1 , it can be seen that the cell morphology in each phycocyanin treatment group is normal, there is no difference from the control group, the cells grow well, and the cell density is higher than that of the control group.

[0064] Application Example 2: Effect of phycocyanin on epidermal water content

[0065] The water-soluble phycocyanin prepared in Example 2 was respectively formulated into 0.5%, 1.0% w / w aqueous solutions for standby. Five volunteers aged 27 - 45 years old rested in a room at 23 - 26 °C and RH 45 - 65% for 30 minutes, and then used 825 to measure the water content (a.u.) of the skin on the inner sides of the left and right forearms. After measurement, different concentrations of phycocyanin solutions were applied, and the application amount was 0.1 mL / 16 cm 2 (4 × 4 cm), allowed to dry naturally, and after resting for 30 minutes, the water content of the skin at the application site was measured. The results are shown in Table 3, and the skin water content data indicate that phycocyanin can increase the skin water content and has a moisturizing effect.

[0066] Table 3 Test values of volunteers' skin water content

[0067]

[0068] Example 3: Preparation of phycocyanin fermented by Lactobacillus

[0069] Using the Lactobacillus bulgaricus strain, which has extracellular protease and cell wall protease activities. The Lb. bulgaricus strain is activated in MRS liquid medium, sampled for culture counting after being cultured to the stationary phase. The viable cell count of this strain in the stationary phase is ~3E10 CFU / mL, stored refrigerated for use within 2 weeks. Prepare the liquid medium (%, w / w): 3.0% of the phycocyanin prepared in Example 1, 1.0% glucose, natural pH, sterilized at 115 °C for 15 min. The MRS culture liquid of the Lb. bulgaricus strain is inoculated into the medium at 2.0% v / v, cultured within the range of 35 - 40 °C for 24 h, and the pH is controlled within the range of 5.5 - 6.0 by feeding alkali solution; after the culture is completed, the temperature is raised to 80 °C and held for 15 min to inactivate the Lactobacillus bulgaricus cells; after centrifuging and separating the supernatant, a specific molecular weight range of components is collected by filtration using a membrane with a specific cut-off molecular weight, and the cut-off molecular weight range is 100 - 20000 Da to obtain the phycocyanin fermentate, and the resulting liquid is named "Lactobacillus / alginate extract fermentation product", and the solid content is about 3 - 4%.

[0070] Application Example 3; Phycocyanin fermentate promotes the production of spermidine in skin microbiota analogs

[0071] Since there is currently no in vitro culture model that is completely equivalent to the human skin microbiota, nor a skin-like structure model with skin microbiota cultured in vitro, in the present invention, an in vitro culture of skin microbiota sampling is used as a skin microbiota analog. After sampling the skin microbiota of volunteers, it is cultured in a liquid medium based on artificial sweat to obtain a skin microbiota analog. The "Lactobacillus / alginate extract fermentation product" based on phycocyanin is added to the culture system of this analog for incubation, and the change in the content of spermidine in the system is detected by sampling at different time points.

[0072] The specific experimental procedure is as follows:

[0073] The liquid medium was composed of 50% artificial sweat (MesGen MAP1065, pH 6.5), 0.1% tryptone, and 0.05% yeast extract powder. After filtration sterilization, it was used. After wiping the faces of volunteers back and forth with a sterile swab, the wiping end was cut off and immersed in the liquid medium. After culturing with shaking at 100 - 150 rpm at 37°C for 24 h, a skin microbiota analogue was obtained and stored refrigerated for later use. According to the ratio of 50% liquid medium, 40% skin microbiota analogue, and 10% v / v of the "Lactobacillus / algal extract fermentation product" prepared in Example 3, a reaction system (treatment group) was formulated and incubated with shaking at 100 - 150 rpm at 37°C. Samples were taken at different time points and the concentration of spermidine in the system was detected by the zinc reagent colorimetric method (Yasumasa Fukushima, 2019); the liquid medium was used to replace the "Lactobacillus / algal extract fermentation product" as a blank control (blank group), and other conditions were the same.

[0074] The results (Table 4) showed that among the skin microbiota analogues from different volunteers, although the skin microbiota analogues of different volunteers had obvious differences in metabolic characteristics, within different time ranges from 0 to 8 h, compared with the blank group, the treatment group added with the "Lactobacillus / algal extract fermentation product" could promote the production of spermidine and increase the concentration of spermidine.

[0075] Table 4 The "Lactobacillus / algal extract fermentation product" promotes the production of spermidine

[0076]

[0077]

[0078] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. Application of phycocyanin or its fermentation products in the preparation of skin care products; The phycocyanin has polyaspartic acid as the main chain and arginine as the side chain, and the molar ratio of aspartic acid to arginine is 1:0.9-1.1; The skin care products include products that help improve skin moisture condition and / or anti-aging.

2. The use according to claim 1, characterized in that The molecular weight of the phycocyanin is less than or equal to 20 kDa; Preferably, the molecular weight of the phycocyanin fermentation product is 100Da-200kDa, more preferably 100Da-20kDa; Preferably, the phycocyanin fermentation product is a fermentation product obtained by fermenting phycocyanin using lactobacillus having protease activity; preferably, the protease includes any one or more of extracellular protease and cell wall protease.

3. The use according to claim 1 or 2, characterized in that: The preparation method of the phycocyanin fermentation product comprises the following steps: Add phycocyanin to a culture medium containing sugars, inoculate lactobacillus into the culture medium, and culture at 30-50° C. for 4-48 hours, controlling the pH value to be 4.0-7.0 during the culture; after the culture is completed, heat the culture medium to inactivate the lactobacillus, centrifuge, and filter to obtain a phycocyanin fermentation product; Preferably, the content of phycocyanin in the culture medium is 1-5% w / w; Preferably, the content of sugars in the culture medium is 0.1-2.0% w / w; Preferably, the sugar is a monosaccharide or a disaccharide; further preferably, the sugar is one of glucose or sucrose; Preferably, the concentration of lactobacilli in the culture medium is E6-1E9 CFU / mL, preferably 1E8-1E9 CFU / mL; Preferably, the culture temperature is 35-45°C and the culture time is 12-24h; Preferably, the inactivation is carried out by heating the mixture to 70-80°C and keeping the temperature for 10-20 minutes; Preferably, the filtration is performed by filtering with a specific molecular weight cut-off membrane to collect components within a specific molecular weight range, wherein the molecular weight range is 100 Da-200 kDa, preferably 100 Da-20 kDa.

4. The use according to claim 1, characterized in that In the phycocyanin, the molar ratio of aspartic acid to arginine is 1:0.95-1.

05.

5. The use according to claim 1, characterized in that The skin care product includes at least one of lotion, emulsion, base lotion, essence, mask, sunscreen, face cream, eye cream, hand cream or body cream; Preferably, the facial mask is a patch-type facial mask or a smear-type facial mask; Preferably, the essence is essence water, essence oil or essence gel.

6. The use according to claim 5, characterized in that The content of phycocyanin in the skin care product is 0.01 to 5.0 wt %, preferably 0.1 to 1.0 wt %.

7. The use according to claim 1, characterized in that Improving the skin's moisture condition includes increasing the skin's moisture content; Preferably, the anti-aging comprises increasing the activity of keratinocytes or increasing the content of spermidine on the skin surface.

8. A skin care product, characterized in that: Contains 0.01-5.0wt% of phycocyanin or its fermentation product, wherein the phycocyanin has polyaspartic acid as the main chain and arginine as the side chain, and the molecular weight is less than or equal to 20kD; the phycocyanin fermentation product is a fermentation product obtained by fermenting phycocyanin with lactobacillus having protease activity, and the molecular weight is 100Da-200kDa.

9. The skin care product according to claim 8, characterized in that The skin care product includes at least one of lotion, emulsion, base lotion, essence, mask, sunscreen, face cream, eye cream, hand cream or body cream; Preferably, the facial mask is a patch-type facial mask or a smear-type facial mask; Preferably, the essence is essence water, essence oil or essence gel.

10. The skin care product according to claim 8, characterized in that The skin care product also includes cosmetically acceptable excipients; Preferably, the auxiliary material is one or more of a solvent, a thickener, a humectant, a film former, a liposome, a preservative, a colorant, a pH adjuster and a flavor; Preferably, the solvent is water or an alcohol solvent.

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