All-natural component lipstick and preparation method thereof

Abstract

The invention discloses an all-natural component lipstick and a preparation method thereof. The all-natural component lipstick is prepared from the following components: alpha-bisabolol, white beeswax, candelilla wax, natural functional components, plant essential oil and the balance of natural grease, the natural functional components consist of a rhodiola rosea extract, vitamin E, a ginseng extract and rhizoma acori graminei oil. Experiments prove that the lipstick with all natural components has remarkable moisturizing effect and antibacterial effect, is suitable for all skin types including sensitive skin, and is a safe, effective and environment-friendly lip care product.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to a lip balm with all-natural ingredients and a preparation method thereof. Background Art

[0002] Currently, most lip balms on the market contain chemically synthesized ingredients, which are extremely likely to cause adverse reactions such as allergies and peeling to the lip skin. Notably, some products contain menthol, which, in addition to bringing a cooling sensation, may also have an inhibitory effect on the central nervous system, posing potential health risks. In addition, most lip balms on the market mainly focus on basic moisturization, but the moisturizing effect is often unsatisfactory, and significant improvement is difficult to achieve even with long-term use.

[0003] With the continuous improvement of the public's health awareness, consumers' demands for lip balm products have shifted from a single aesthetic effect to a dual pursuit of health and safety. The market demand for safe and effective lip balms is increasing day by day.

[0004] In addition, synthetic biology can efficiently produce natural compounds that are difficult to obtain by traditional methods, such as α-bisabolol and squalene, through genetic engineering and metabolic engineering means. However, there is currently no report on preparing lip balm using the biosynthetic raw material α-bisabolol as a raw material. Summary of the Invention

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a lip balm with all-natural ingredients that has significant moisturizing and antibacterial effects.

[0006] The second purpose of the present invention is to provide a preparation method of a lip balm with all-natural ingredients.

[0007] The technical solution of the present invention is outlined as follows:

[0008] A lip balm with all-natural ingredients is made of the following components by mass percentage: 0.5% - 1% of α-bisabolol, 15% - 20% of white beeswax, 4% - 10% of candelilla wax, 4% - 10% of natural functional ingredients, 0.5% - 1% of plant essential oil, and the balance is natural oil; the natural functional ingredients are composed of rhodiola rosea extract, vitamin E, ginseng extract, and acorus tatarinowii oil; the α-bisabolol is a biosynthetic raw material, which is obtained by fermenting a genetically engineered yeast strain with a mutant recombinant plasmid, and has naturalness and sustainability.

[0009] Preferably, the natural functional ingredients are composed of rhodiola rosea extract, vitamin E, ginseng extract, and acorus tatarinowii oil in a mass ratio of 1:1:1:1.

[0010] Preferably, the plant essential oil is sweet orange essential oil, rose essential oil, lemon essential oil, or lavender essential oil.

[0011] Preferably, the natural oil is sweet almond oil, perilla seed oil or shea butter.

[0012] A method for preparing a lip balm with all-natural ingredients, comprising the following steps:

[0013] 1) Weigh by mass percentage: 0.5% - 1% of α-bisabolol, 15% - 20% of white beeswax, 4% - 10% of candelilla wax, 4% - 10% of natural functional ingredients, 0.5% - 1% of plant essential oil, and the balance is natural oil; the natural functional ingredients are composed of rhodiola rosea extract, vitamin E, ginseng extract and acorus tatarinowii oil; the α-bisabolol is obtained by fermentation of a genetically engineered yeast strain with a mutated recombinant plasmid;

[0014] 2) Mix the α-bisabolol, natural functional ingredients, plant essential oil and natural oil in step 1) evenly and grind for 30 - 40 minutes;

[0015] 3) Heat up to 70 - 90 °C, melt the white beeswax and candelilla wax into a liquid, add it to the material obtained in step 2), defoam using a vacuum defoamer, filter, pour the filtrate into a mold, cool, demold, and obtain a lip balm with all-natural ingredients.

[0016] Preferably, the natural functional ingredients are composed of rhodiola rosea extract, vitamin E, ginseng extract and acorus tatarinowii oil in a mass ratio of 1:1:1:1.

[0017] Preferably, the plant essential oil is sweet orange essential oil, rose essential oil, lemon essential oil or lavender essential oil.

[0018] Preferably, the natural oil is sweet almond oil, perilla seed oil and shea butter.

[0019] Advantages of the present invention:

[0020] The raw materials of the lip balm with all-natural ingredients of the present invention are selected from synthetic biology raw materials and natural extracts, without any chemical additives, with simple ingredients and not easily causing allergic reactions.

[0021] α-Bisabolol, as a synthetic biology raw material, is synthesized by genetically engineered Saccharomyces cerevisiae using renewable bio-based raw materials in its synthesis process. It features low energy consumption and less pollution during production, conforming to the concept of sustainable development and reducing the environmental burden. Replacing the chemical raw materials added in similar products on the market, it not only has good efficacy but also ensures its naturalness and green safety, greatly reducing the occurrence of lip problems for users. It has biological activities such as anti-inflammatory, antibacterial, and anti-irritant, can reduce skin inflammation, has anti-allergic effects, and can improve the skin's anti-irritant ability. It is a natural ingredient with good stability and high biocompatibility with the skin. In addition, α-bisabolol produced by synthetic biology technology has high production efficiency and stability, can meet the needs of large-scale production, and ensure the continuous supply of raw materials.

[0022] Rhodiola rosea extract has the effects of enhancing immune function, anti-fatigue, and repairing and protecting the body.

[0023] Vitamin E is a powerful antioxidant that can help repair damaged skin cells and reduce lip chapping.

[0024] Ginseng extract contains ginsenosides and other active ingredients, which can maintain the healthy state of cells, promote collagen production and improve cell activity, fade lip lines, promote epidermal cell renewal, and maintain the youthful state of the lip muscles.

[0025] Acorus tatarinowii oil has the effect of promoting blood circulation to remove blood stasis, can effectively promote lip blood circulation, prevent the precipitation of melanin, and at the same time has the effects of calming the nerves and opening the orifices to restore consciousness, and has a good protective effect on brain tissue and nerve cells.

[0026] Sweet almond oil, perilla seed oil, and shea butter, as natural oils, all contain essential fatty acids and vitamins, which can provide nourishment and moisture for lip skin, help lock in moisture, prevent dryness, and at the same time can help repair damaged lip skin and reduce lip chapping.

[0027] Experiments have proved that a lip balm with all-natural ingredients of the present invention has significant moisturizing and antibacterial effects, is suitable for all skin types including sensitive skin, and is a safe, effective, and environmentally friendly lip care product. Description of the Drawings

[0028] Figure 1 It is the antibacterial experiment of a lip balm with all-natural ingredients against Escherichia coli. From left to right are the antibacterial effects of Examples 6 to 10.

[0029] Figure 2 It is the antibacterial experiment of a lip balm with all-natural ingredients against Staphylococcus aureus. From left to right are the inhibitory effects of Examples 6 to 10. Detailed Description of the Invention

[0030] The present invention will be further described below in conjunction with specific embodiments.

[0031] The embodiments of the present invention are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

[0032] The source information of the raw materials involved in the following embodiments is shown in Table 1: The source of each raw material in Table 1 is only for the convenience of those skilled in the art to implement, but it is not limited thereto. Similar raw materials produced by other enterprises can also be used in the present invention.

[0033] Table 1

[0034]

[0035] Preparation of natural functional components: Weigh the same mass of rhodiola rosea extract, vitamin E, ginseng extract, and acorus tatarinowii oil, and mix them evenly.

[0036] The raw material ratios of Examples 1 - 5 are shown in Table 2.

[0037] Table 2

[0038]

[0039] Example 6

[0040] A method for preparing a fully natural ingredient lip balm, comprising the following steps:

[0041] 1) Weigh the raw materials according to Example 1 of Table 2;

[0042] 2) Mix α-bisabolol, natural functional components, plant essential oils, and natural oils in step 1) evenly, and grind for 40 minutes to make them uniform;

[0043] 3) Heat up to 70 °C, melt white beeswax and candelilla wax into a liquid, add it to the material obtained in step 2), use a vacuum degassing machine to degas, filter, pour the filtrate into a mold, cool, and demold to obtain a fully natural ingredient lip balm.

[0044] Example 7

[0045] A method for preparing a fully natural ingredient lip balm, comprising the following steps:

[0046] 1) Weigh the raw materials according to Example 2 of Table 2;

[0047] 2) Mix α-bisabolol, natural functional components, plant essential oils, and natural oils in step 1) evenly, and grind for 35 minutes to make them uniform;

[0048] 3) Heat up to 80 °C, melt white beeswax and candelilla wax into liquid, add them to the material obtained in step 2), defoam using a vacuum defoaming machine, filter, pour the filtrate into a mold, cool, and demold to obtain a lip balm with all-natural ingredients.

[0049] Example 8

[0050] A method for preparing a lip balm with all-natural ingredients, comprising the following steps:

[0051] 1) Weigh the raw materials according to Example 3 in Table 2;

[0052] 2) Mix α-bisabolol, natural functional ingredients, plant essential oils, and natural oils in step 1) evenly, and grind for 30 minutes to make them uniform;

[0053] 3) Heat up to 90 °C, melt white beeswax and candelilla wax into liquid, add them to the material obtained in step 2), defoam using a vacuum defoaming machine, filter, pour the filtrate into a mold, cool, and demold to obtain a lip balm with all-natural ingredients.

[0054] Example 9

[0055] A method for preparing a lip balm with all-natural ingredients, comprising the following steps:

[0056] 1) Weigh the raw materials according to Example 4 in Table 2;

[0057] 2)-3) The same as 2)-3) in Example 7.

[0058] Example 10

[0059] A method for preparing a lip balm with all-natural ingredients, comprising the following steps:

[0060] 1) Weigh the raw materials according to Example 5 in Table 2;

[0061] 2)-3) The same as 2)-3) in Example 7.

[0062] Before entering vacuum defoaming in Examples 6-10, different proportions of natural pigments (such as natural cochineal) can also be added to make colored lip balms with all-natural ingredients in different colors.

[0063] Experimental Example 1 Antibacterial Experiment

[0064] The antibacterial activity of the lip balm with all-natural ingredients was measured by the disc diffusion method.

[0065] Pour the nutrient agar medium into a sterile petri dish to make a plate: After cooling, divide it into 3 equal areas. Pipette 100 μl of the Escherichia coli suspension of ATCC 25922 and the Staphylococcus aureus suspension of ATCC 6538 onto the above plate and spread them. Dry at room temperature for 5 min. Melt the all-natural ingredient lipsticks prepared in Examples 6 - 10 into a liquid state. Use sterile forceps to pick up filter paper discs with a diameter of 5 mm and immerse them in the melted lipsticks. Stick the impregnated filter paper discs on the corresponding 3 areas of the plate and place them in an incubator at 37°C for cultivation. After 24 h, take out the plate and observe the bacterial growth status around the filter paper discs in the 3 areas. Judge its antibacterial effect by the diameter of the inhibition zone. If the diameter of the inhibition zone is greater than 6 mm, it is determined to have an antibacterial effect; if the diameter of the inhibition zone is less than 6 mm, it is determined to have no antibacterial effect.

[0066] The experimental results are shown in Figure 1 、 Figure 2 and Table 3: Figure 1 From left to right, corresponding to the inhibition zones of the all-natural ingredient lipsticks of Examples 6 - 10 in Table 3 against Escherichia coli, Figure 2 From left to right, corresponding to the inhibition zones of the all-natural ingredient lipsticks of Examples 6 - 10 against Staphylococcus aureus.

[0067] It can be seen from Table 3 that the lipsticks of the present invention have good antibacterial effects.

[0068] Table 3 Statistical results of antibacterial effects

[0069] Example 6 Example 7 Example 8 Example 9 Example 10 Inhibitory zone of Escherichia coli 2.03 cm 2.09 cm 2.07 cm 1.97 cm 1.99 cm Inhibitory zone of Staphylococcus aureus 2.17 cm 2.29 cm 2.20 cm 2.05 cm 2.10 cm

[0070] Experimental Example 2

[0071] Stability test

[0072] Place the all-natural ingredient lipsticks prepared in Examples 6 - 10 in an incubator at 35°C and a refrigerator at -20°C for 12 h respectively. After taking them out, the states of all the all-natural ingredient lipsticks do not show oiling, deformation, or delamination; therefore, it is proved that the all-natural ingredient lipsticks prepared in Examples 6 - 10 are stable under temperature changes.

[0073] Experimental Example 3

[0074] Effect evaluation of an all-natural ingredient lipstick

[0075] Test subjects: Recruit 50 volunteers, all female, including female college students, working women, and housewives, aged 20 - 55 years old. Randomly divide them into five groups evenly, with 10 people in each group.

[0076] Lipstick samples: One kind of all-natural ingredient lipsticks prepared in Examples 6 - 10, 10 pieces each.

[0077] Usage frequency: 2 times per person per day (apply lip balm to cover the lip surface), for a total of 90 days.

[0078] The total score for each usage effect is 5 points:

[0079] 5 points is the highest score, indicating very good and very satisfied;

[0080] 4 points means relatively good;

[0081] 3 points means acceptable;

[0082] 2 points means not good,

[0083] 1 point means unacceptable.

[0084] After three months of testing, the results in Table 4 below were obtained:

[0085] Table 4 Effect Evaluation Statistics

[0086] Example 6 Example 7 Example 8 Example 9 Example 10 Wrinkle reduction effect 3.6 3.4 3.7 4.1 4.8 Moisturizing effect 4.2 4 3.9 4.3 3.7 Gentleness level 5 5 5 5 5

Claims

1. A lipstick with all-natural ingredients, characterized in that The invention is prepared from the following components by mass percentage: 0.5% to 1% α-bisabolol, 15% to 20% white beeswax, 4% to 10% candelilla wax, 4% to 10% natural functional ingredients, 0.5% to 1% plant essential oil, and the balance is natural oil; the natural functional ingredients are composed of rhodiola rosea extract, vitamin E, ginseng extract and calamus oil; the α-bisabolol is obtained by fermenting brewer's yeast genetic engineering bacteria with a mutated recombinant plasmid.

2. The all-natural lipstick according to claim 1, characterized in that The natural functional ingredients consist of rhodiola rosea extract, vitamin E, ginseng extract and calamus oil in a mass ratio of 1:1:1:

1.

3. The all-natural lipstick according to claim 1, characterized in that The plant essential oil is sweet orange essential oil, rose essential oil, lemon essential oil or lavender essential oil.

4. The all-natural lipstick according to claim 1, characterized in that The natural oil is sweet almond oil, perilla seed oil or avocado tree butter.

5. A method for preparing a lipstick containing all-natural ingredients, characterized in that The steps include: 1) Weighing by mass percentage: 0.5%-1% of α-bisabolol, 15%-20% of white beeswax, 4%-10% of candelilla wax, 4%-10% of natural functional ingredients, 0.5%-1% of plant essential oil, and the balance of natural oils and fats; the natural functional ingredients are composed of rhodiola rosea extract, vitamin E, ginseng extract and calamus oil; the α-bisabolol is obtained by fermentation of genetically engineered yeast of mutated recombinant plasmid; 2) mixing α-bisabolol, natural functional ingredients, plant essential oils, and natural oils in step 1) evenly and grinding for 30-40 minutes; 3) heating to 70-90° C., melting white beeswax and candelilla wax into liquid, adding the liquid to the material obtained in step 2), degassing with a vacuum degassing machine, filtering, pouring the filtrate into a mold, cooling, and demolding to obtain an all-natural lipstick.

6. The preparation method according to claim 5, characterized in that The natural functional ingredients consist of rhodiola rosea extract, vitamin E, ginseng extract and calamus oil in a mass ratio of 1:1:1:

1.

7. The preparation method according to claim 5, characterized in that The plant essential oil is sweet orange essential oil, rose essential oil, lemon essential oil or lavender essential oil.

8. The preparation method according to claim 5, characterized in that The natural oils and fats are sweet almond oil, perilla seed oil and avocado tree butter.

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