Egg laying synergistic composite additive for laying hens and preparation method thereof

Abstract

The invention provides an egg laying synergistic composite additive for laying hens, which comprises the following components in parts by weight: 15-25 parts of alfalfa meal, 8-15 parts of dandelion extract, 10-18 parts of purslane powder, 12-20 parts of pine needle meal, 6-12 parts of folium artemisiae argyi powder, 10-18 parts of astragalus extract, 8-15 parts of codonopsis pilosula extract, 5-10 parts of angelica sinensis extract, 6-12 parts of motherwort extract, 4-8 parts of acanthopanax senticosus extract and 50-80 parts of a carrier. The enteric coated pellets are based on conventional pellets, polysaccharide fragments on the surfaces of the enteric coated pellets are combined with intestinal epithelial cell receptors, plant extracts are accurately released, intestinal health is maintained, the pellets are wrapped by the plant extracts and mixed with other components during stirring, and the effects of resisting bacteria, enhancing immunity and the like are achieved by means of targeted transportation of the pellets. The health and egg laying performance of the laying hens are improved, the pellet polysaccharide fragments maintain intestinal epithelial cell functions, the plant extracts regulate intestinal flora and improve intestinal microecology, and digestion, absorption and egg laying efficiency of the laying hens are guaranteed.

Description

Technical Field

[0001] The present invention belongs to the field of additive processing, and specifically relates to a compound additive for enhancing egg production in laying hens and a preparation method thereof. Background Art

[0002] Additives made from herbal plants can significantly improve the health and egg production performance of laying hens. It can supplement comprehensive nutrition for laying hens, enhance the body's immunity, effectively resist the invasion of pathogens, reduce the occurrence of diseases. At the same time, it can regulate the intestinal microecology of laying hens, promote digestion and absorption, and ensure nutrient supply. In addition, it can also improve the function of the reproductive system, promote follicle development, improve the quality and production rate of eggs, and help laying hens achieve efficient production.

[0003] For example, a feed additive for improving the egg production performance of laying hens and a preparation method thereof disclosed in the patent document CN115918783B are prepared from the following components in parts by weight: 13 - 18 parts of Caulis Spatholobi, 18 - 23 parts of Radix Panacis Quinquefolii, 6 - 12 parts of Semen Allii Tuberosi, 10 - 15 parts of Rosa rugosa Thunb. f. plena, 44 - 49 parts of malt beer distillers' grains. The feed additive prepared by the present invention is added and mixed evenly in the complete diet during the breeding of laying hens. It can not only enable laying hens to exert the maximum egg production performance, but also ensure their physiological health, reduce the incidence and mortality. However, it is composed of Caulis Spatholobi, Radix Panacis Quinquefolii, Semen Allii Tuberosi, Rosa rugosa Thunb. f. plena and malt beer distillers' grains. However, the cost of Radix Panacis Quinquefolii is high, and the malt beer distillers' grains contain alcohol, which will cause abnormal egg production in laying hens; and there is a lack of synergistic effect among the components to improve the egg production performance and health of laying hens. At the same time, the release of the additive itself lacks precision and cannot exert its effect on specific areas of the intestinal tract of laying hens. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides a compound additive for enhancing egg production in laying hens and a preparation method thereof to solve the problems raised in the background art.

[0005] A compound additive for enhancing egg production in laying hens includes the following components in parts by weight: 15 - 25 parts of alfalfa meal, 8 - 15 parts of dandelion extract, 10 - 18 parts of purslane powder, 12 - 20 parts of pine needle powder, 6 - 12 parts of mugwort leaf powder, 10 - 18 parts of astragalus extract, 8 - 15 parts of codonopsis pilosula extract, 5 - 10 parts of angelica extract, 6 - 12 parts of motherwort extract, 4 - 8 parts of acanthopanax extract, and 50 - 80 parts of a carrier.

[0006] Preferably, the sum of the parts by weight of the alfalfa meal and the dandelion extract is 30 - 35 parts.

[0007] Preferably, the total sum of the parts by weight of the purslane powder, the pine needle powder, and the mugwort leaf powder is 32 - 40 parts.

[0008] Preferably, the weight ratio of the astragalus extract, codonopsis pilosula extract, and angelica sinensis extract is 1.8 - 2.2:1.4 - 1.6:1.

[0009] Preferably, the carrier is a starch-based carrier treated by spray drying, and its water content is less than 5%.

[0010] Preferably, the weight ratio of the acanthopanax senticosus extract to the carrier is 1:10 - 1:12.

[0011] Preferably, the weight of the dandelion extract, motherwort extract, and acanthopanax senticosus extract satisfies: dandelion extract - motherwort extract + acanthopanax senticosus extract = 10 - 12 parts.

[0012] A preparation method of a compound additive for enhancing egg production in laying hens includes the following steps:

[0013] S1: Select specific plant raw materials without mildew and less impurities, rinse them 3 - 5 times with flowing clear water to remove impurities, put them into a blast drying oven at 60 - 70 °C, dry for 8 - 10 hours until the water content is less than 10%, and then crush them to 80 - 100 meshes with a high-speed crusher to ensure uniform particles, completing the pretreatment and preparing for subsequent extraction;

[0014] S2: Put the pretreated and crushed raw materials into an ultrasonic extraction device, add an 8 - 10-fold volume of ethanol solution with a volume fraction of 70% - 80%, perform ultrasonic extraction at 40 - 50 °C and a power of 300 - 400 W for 30 - 40 minutes, and after extraction, concentrate it with a decompression concentration device at 60 - 70 °C and a vacuum degree of 0.08 - 0.09 MP to obtain a concentrated plant extract solution for standby;

[0015] S3: Take an appropriate amount of probiotic preparations such as bacillus subtilis and bifidobacterium, inoculate them into a liquid medium containing specific nutrient components and ratios, adjust the pH to 7.0 - 7.2, place them in a constant temperature shaker at 37 °C, and oscillate and culture at a rotation speed of 150 - 200 r / min for 12 - 16 hours to make the viable bacteria count not less than 1×10 9 CFU / mL, completing the preparation for standby;

[0016] S4: Accurately weigh vitamins, minerals, amino acids, etc. with an electronic balance, and premix them into a basic mixture by low-speed stirring for 10 - 15 minutes. During stirring, simultaneously add the concentrated plant extract solution and specially modified enteric-coated pellets;

[0017] S5: Slowly add the concentrated plant extract solution to the basic mixture, increase the stirring speed to 300 - 400 r / min, stir for 20 - 30 minutes, utilize the liquid-solid mixing characteristics to promote wrapping and infiltration, and for components such as those prone to oxidation, make microcapsules with edible biopolymer materials to wrap them, and trigger dissolution and fusion under specific conditions;

[0018] S6: Add the activated probiotic preparation, adjust the stirring speed to 50 - 100 r / min, stir for 15 - 20 minutes to ensure uniform distribution without damage, maintain a temperature of 10 - 15 °C throughout the mixing process, maintain sterility with the help of an air purification device, and use intelligent control to ensure that the parameters meet the standards to obtain the composite additive.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. In the use of the present invention, the enteric-coated pellets are based on conventional pellets and also include acrylic resin, ensuring stability in gastric acid and the ability to dissolve in the alkaline intestinal environment. Moreover, the polysaccharide fragments on its surface bind to intestinal epithelial cell receptors to precisely release the plant extract, maintaining intestinal health. When stirring, the plant extract wraps around the pellets and mixes with other components. With the help of the targeted transportation of the pellets, it exerts antibacterial, immune-enhancing and other effects, improving the health and egg-laying performance of laying hens. The polysaccharide fragments of the pellets maintain the function of intestinal epithelial cells, and the plant extract regulates the intestinal flora, improving the intestinal microecology, and ensuring the digestion, absorption and egg-laying efficiency of laying hens.

[0021] 2. In the use of the present invention, liquid-solid mixing promotes affinity, and the microcapsules wrap and protect the easily oxidized components. Specific temperature and pH trigger dissolution, enabling the components to stably fuse, reducing adverse reactions between components, improving the stability and effectiveness of the additive, and ensuring that each component plays its best role.

[0022] 3. In the use of the present invention, low-speed stirring prevents damage to live bacteria, ensures uniform distribution of probiotics, and environmental control and real-time monitoring ensure the mixing quality, timely adjust deviations, ensure the quality stability of each batch of additive, and improve the overall performance of the additive. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic structural diagram of the flow chart for processing plant raw materials of the compound additive for enhancing egg production efficiency of laying hens in a specific embodiment of the present invention;

[0024] Figure 2 It is a schematic structural diagram of the flow chart for activating and culturing probiotics of the compound additive for enhancing egg production efficiency of laying hens in a specific embodiment of the present invention;

[0025] Figure 3 It is a schematic structural diagram of the flow chart for mixing and preparing components of the compound additive for enhancing egg production efficiency of laying hens in a specific embodiment of the present invention. SPECIFIC EMBODIMENTS

[0026] The following further describes in detail the embodiments of the present invention in conjunction with the drawings and examples. The following examples are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0027] A compound additive for improving egg production efficiency in laying hens, comprising the following components in parts by weight: 15-25 parts of alfalfa meal, 8-15 parts of dandelion extract, 10-18 parts of purslane powder, 12-20 parts of pine needle powder, 6-12 parts of mugwort powder, 10-18 parts of astragalus extract, 8-15 parts of codonopsis pilosula extract, 5-10 parts of angelica extract, 6-12 parts of motherwort extract, 4-8 parts of acanthopanax senticosus extract, and 50-80 parts of a carrier.

[0028] Specifically, alfalfa meal is rich in high-quality plant protein, providing the basic raw materials for the construction and repair of the laying hen's body tissues, and contains a variety of vitamins (such as vitamins A, D, E, K groups and B-group vitamins) that participate in the synthesis and metabolism of various enzymes in the body, as well as abundant minerals such as calcium, phosphorus, iron, and zinc. Among them, calcium and phosphorus are related to eggshell formation, iron participates in oxygen transport, and zinc affects the development of the reproductive system and immune function; the active ingredients such as taraxasterol and taraxacin in dandelion extract can inhibit the growth of pathogenic bacteria such as Escherichia coli and Staphylococcus aureus, regulate the inflammatory signaling pathway to reduce inflammation, and promote the decomposition and excretion of toxins in the body; purslane powder contains antioxidant vitamins such as vitamin C, E, and β-carotene to scavenge free radicals and enhance antioxidant capacity, and its minerals such as potassium, magnesium, and calcium play roles in maintaining osmotic pressure balance, activating enzymes, and ensuring the health of eggshells and bones; pine needle powder contains more than 18 kinds of amino acids, including a variety of essential amino acids, providing raw materials for laying hens to synthesize biological macromolecules, and is rich in various vitamins, which help in maintaining visual and reproductive system functions, enhancing immunity and antioxidant capacity, and improving egg production performance; the volatile oil of mugwort powder, such as cineole and camphor, has antibacterial effects and regulates the intestinal microecology, and its active ingredients can also promote blood circulation and provide nutrients for various organs of the body; astragalus polysaccharide in astragalus activates immune cells, enhances immune responses, and promotes antibody production; codonopsis saponins and polysaccharides in codonopsis pilosula regulate hormone levels, improve digestive function, and increase feed conversion rate; ferulic acid and angelica polysaccharide in angelica regulate the reproductive endocrine system, promote follicle development, improve oviduct function, and enhance egg quality; leonurine in motherwort promotes the development of reproductive organs, regulates the secretion of reproductive hormones, improves the function of reproductive organs, and increases egg production rate; acanthopanax polysaccharide and acanthopanax glycosides in acanthopanax senticosus regulate the level of stress hormones, enhance the regulatory function of the nervous system, and improve the adaptability of laying hens to environmental changes.

[0029] In addition, the carrier can not only effectively carry other active ingredients, but also ensure the uniform distribution of the additive in the feed, improve the stability and bioavailability of each component, enable laying hens to more fully absorb and utilize nutrients, and exert the maximum efficacy of the additive.

[0030] A preparation method of a compound additive for improving egg production efficiency in laying hens, comprising the following steps:

[0031] S1: Select specific plant raw materials without mildew and few impurities, rinse them 3 - 5 times with flowing clean water to remove impurities, put them into a blast drying oven at 60 - 70 °C, dry for 8 - 10 hours until the moisture content is below 10%, and then crush them to 80 - 100 meshes with a high-speed crusher to ensure uniform particles, completing the pretreatment and preparing for subsequent extraction;

[0032] S2: Put the pretreated and crushed raw materials into an ultrasonic extraction device, add an 8 - 10 times volume of ethanol solution with a volume fraction of 70% - 80%, and perform ultrasonic extraction at 40 - 50 °C and a power of 300 - 400 W for 30 - 40 minutes. After extraction, concentrate it with a vacuum concentration device at 60 - 70 °C and a vacuum degree of 0.08 - 0.09 MP to obtain a concentrated plant extract solution for standby;

[0033] S3: Take appropriate amounts of probiotic preparations such as Bacillus subtilis and Bifidobacterium, inoculate them into a liquid medium containing specific nutrient components and ratios, adjust the pH to 7.0 - 7.2, place them in a constant temperature shaker at 37 °C, and oscillate and culture at a rotation speed of 150 - 200 r / min for 12 - 16 hours to make the viable count not less than 1×10 9 CFU / mL, completing the preparation for standby;

[0034] S4: Accurately weigh vitamins, minerals, amino acids, etc. with an electronic balance, and premix them into a basic mixture by low-speed stirring for 10 - 15 minutes. During stirring, simultaneously add the concentrated plant extract solution and specially modified enteric-coated pellets;

[0035] When stirring the basic mixture, simultaneously add the concentrated plant extract solution and enteric-coated pellets modified by a special process. These pellets are based on conventional pellets, coated with acrylic resin on the outside, and incorporated with polysaccharide fragments of beneficial microorganisms in the laying hen's intestine to construct a targeted delivery carrier for the plant extract. This process realizes the preliminary uniform distribution of components such as vitamins in the basic mixture, ensures that each component fully exerts its synergistic effect in the subsequent process, and improves the overall efficiency of the compound additive. At the same time, with the special structure and components of the enteric-coated pellets, the plant extract remains intact in the acidic environment of the laying hen's stomach and is precisely and slowly released to the target site in the alkaline environment of the intestine, significantly improving the effect on the laying hen's intestine and enhancing the improvement effect on the laying hen's health and egg production performance.

[0036] S5: Slowly add the concentrated plant extract solution to the basic mixture, increase the stirring speed to 300 - 400 r / min, and stir for 20 - 30 minutes. Utilize the liquid-solid mixing characteristics to promote wrapping and infiltration. For components such as those prone to oxidation, encapsulate them with edible biopolymer materials to trigger dissolution and fusion under specific conditions;

[0037] For the components in vitamins, minerals, and amino acids that are prone to oxidation and interaction, microcapsules are made of edible biopolymer materials for encapsulation, and triggered to dissolve under the conditions of 30 - 35°C and a pH value of 6.5 - 7.0. This operation not only protects the stability of sensitive components during preparation and storage, but also ensures their slow and precise release and fusion in a suitable environment. High-speed stirring improves the uniformity of component dispersion and the tightness of binding, enhances the stability and synergistic effect of the composite additive, and helps it efficiently promote egg production in laying hens; the microcapsule encapsulation technology guarantees the activity of sensitive components, precisely controls their release, and improves the pertinence and effectiveness of the composite additive in improving the health and egg production performance of laying hens.

[0038] S6: Add the activated probiotic preparation, adjust the stirring speed to 50 - 100 r / min, and stir for 15 - 20 minutes to ensure uniform distribution and no damage. Maintain 10 - 15°C throughout the mixing process, maintain sterility with the help of an air purification device, and use intelligent control to ensure that the parameters meet the standards to obtain the composite additive;

[0039] After the preliminary construction of the previous mixing system is completed, add the activated probiotic preparation, and precisely adjust the stirring speed to 50 - 100 r / min, and stir for 15 - 20 minutes to ensure the uniform distribution of probiotics in the entire composite additive system, while avoiding damage to live bacteria by high-speed stirring, maintaining their activity, and ensuring the subsequent probiotic function. Throughout the mixing process, strictly control the environmental temperature at 10 - 15°C, and maintain a sterile environment with the help of a professional air purification device. Through the sensors and intelligent control system integrated in the mixing equipment, key parameters such as component ratio and particle size distribution are monitored and regulated in real time to ensure that the preparation process meets the preset quality standards, and strict environmental control and intelligent regulation reduce external interference, ensure the quality stability and batch uniformity of the composite additive, enhance the product reliability and market competitiveness, and provide strong support for improving the health and egg production of laying hens.

[0040] Specifically, select plant raw materials such as Astragalus membranaceus, Angelica sinensis, and Leonurus japonicus, and use professional impurity detection instruments to ensure that the raw materials are free of mildew and the impurity content is less than 0.5%. Rinse with flowing water at a flow rate of 2 liters per second for 3 times, and then place them in a blast drying oven at 65°C for 8 hours. With the help of a high-precision moisture detector, accurately reduce the moisture content to 8%. Use a high-speed crusher with a motor speed of 20,000 revolutions per minute, equipped with an 80-mesh sieve, to crush the dried raw materials to 80 - 100 meshes;

[0041] Secondly, put the crushed raw materials into an ultrasonic extraction device, and accurately add 9 times the volume of 75% ethanol solution by mass-volume ratio. At 45 °C, extract with an ultrasonic power of 350 W for 35 minutes, and set the ultrasonic frequency to 40 kHz. After the extraction is completed, concentrate under the conditions of 65 °C and a vacuum degree of 0.085 MP through a vacuum concentration device, and use an online concentration detector to control the concentration of the concentrated solution at the preset 1.2 g / mL;

[0042] Specifically, probiotics such as Bacillus subtilis and Bifidobacterium are inoculated into a liquid medium containing 2.00% glucose, 1.00% peptone, 0.50% beef extract, 0.50% sodium chloride, and a pH value of 7.1. Place the inoculated medium in a constant temperature shaker at 37 °C and shake it at a speed of 180 r / min for 14 hours. Use the plate counting method to make the viable bacteria count reach 1×10 9 CFU / mL;

[0043] Specifically, use a high-precision balance with an accuracy of 0.0001 g to weigh the nutritional components, place them in a low-speed stirrer, and stir at a speed of 120 r / min for 12 minutes to complete the preliminary premixing. The enteric-coated pellets are based on conventional pellets and use the fluidized bed coating technology. The coating thickness is controlled with enteric acrylic resin at 20 μm, and the polysaccharide fragments extracted from the cell walls of symbiotic beneficial microorganisms in the intestinal tract of laying hens are incorporated into the coating at a ratio of 0.5%. During stirring, through an automated addition device, the modified enteric-coated pellets and the plant extract concentrate are added to the base mixture simultaneously.

[0044] In addition, slowly add the plant extract concentrate to the base mixture through a peristaltic pump at a flow rate of 5 mL per minute, and at the same time increase the stirring speed to 350 r / min and stir for 25 minutes. For easily oxidized and interacting components, use the spray drying method to microencapsulate them with chitosan, and control the microcapsule particle size at 50 μm. Trigger the dissolution of the microcapsules in an environment of 32 °C and a pH value of 6.8.

[0045] Example 1:

[0046] Taking the production of 10 kg of additive as an example, the main raw materials selected are:

[0047] 970.8 g of alfalfa meal;

[0048] 582.48 g of dandelion extract;

[0049] 728.1 g of purslane powder;

[0050] 776.64 g of pine needle powder;

[0051] 436.86 g of mugwort powder;

[0052] 679.56 g of astragalus extract;

[0053] 582.48 g of Codonopsis pilosula extract;

[0054] 388.32 g of Angelica sinensis extract;

[0055] 436.86 g of Leonurus japonicus extract;

[0056] 291.24 g of Eleutherococcus senticosus extract;

[0057] 3155.1 g of carrier;

[0058] Operate on the raw materials prepared in the above embodiments according to the established process preparation steps.

[0059] Example 2:

[0060] Taking the production of 10 kg of additive as an example, the main raw materials selected are:

[0061] 1058.76 g of alfalfa meal;

[0062] 588.2 g of Taraxacum mongolicum extract;

[0063] 764.66 g of Portulaca oleracea powder;

[0064] 823.48 g of pine needle powder;

[0065] 470.56 g of Artemisia argyi powder;

[0066] 705.84 g of Astragalus membranaceus extract;

[0067] 588.2 g of Codonopsis pilosula extract;

[0068] 411.74 g of Angelica sinensis extract;

[0069] 470.56 g of Leonurus japonicus extract;

[0070] 294.1 g of Eleutherococcus senticosus extract;

[0071] 3823.3 g of carrier.

[0072] S1: Pretreatment of plant raw materials: Brush plant raw materials such as Astragalus membranaceus with a soft brush under running water to remove impurities. After washing, spread them flat on a drying tray, 2 - 3 cm thick, and dry them in a hot air circulation drying oven at 50 - 60 °C for 4 - 6 hours, turning them over once per hour until the water content is below 10%. Put them into a pulverizer and pulverize them with an 80 - 100 mesh sieve, and collect them in a clean and sealed container to prevent moisture absorption and secondary pollution.

[0073] S2: Add deionized water to the crushed raw materials at a liquid-to-solid ratio of 1:8 - 1:10, and perform micro-boiling extraction in a multi-functional extraction tank at 80 - 90 °C for 2 - 3 hours, with stirring every 30 minutes. Filter while it is hot, extract the residue once again, and combine the filtrates. In a vacuum concentrator, perform vacuum concentration at -0.06 to -0.08 MPa and 50 - 60 °C until the volume is reduced to 1 / 5 - 1 / 4 of the original volume, obtaining a concentrated solution with a relative density of 1.15 - 1.20 (60 °C), and cool it for standby.

[0074] S3: Prepare suitable culture media for Bacillus subtilis and Bifidobacterium respectively. Use nutrient broth peptone medium for Bacillus subtilis and modified MRS medium for Bifidobacterium. After preparation, dispense them into Erlenmeyer flasks and sterilize at 121 °C under high pressure for 15 - 20 minutes.

[0075] S4: Inoculate the freeze-dried powders of the two bacteria into the corresponding activation culture media respectively. Culture Bacillus subtilis at 37 °C for 18 - 24 hours, and culture Bifidobacterium anaerobically at 37 °C for 24 - 36 hours. When the bacterial liquid becomes turbid and there are many viable bacteria under microscopic examination, transfer them to the enlarged culture medium at an inoculation amount of 1:10 - 1:15 and culture until the number of viable bacteria reaches the standard.

[0076] S5: Weigh vitamins, minerals, amino acids, etc. using an electronic balance with a precision of 0.01 g, and place them on clean weighing papers respectively. Pour them into a stirring kettle, stir at 100 - 150 revolutions per minute, slowly add the concentrated plant extract, stir for 15 - 20 minutes, then add the activated probiotic preparation, and stir at 80 - 120 revolutions per minute for 30 - 40 minutes, and store in a sterile container after sealing.

[0077] Operate on the raw materials prepared in the above embodiments according to the general process preparation steps given in this embodiment.

[0078] Example 3:

[0079] Taking the production of 10 kg of additive as an example, the main raw materials selected are:

[0080] 1071.45 g of ordinary alfalfa meal;

[0081] 571.44 g of ordinary dandelion extract;

[0082] 714.3 g of ordinary purslane powder;

[0083] 857.16 g of ordinary pine needle powder;

[0084] 428.58 g of ordinary mugwort powder;

[0085] 714.3 g of ordinary astragalus extract;

[0086] 571.44 g of ordinary codonopsis pilosula extract;

[0087] 357.15 g of ordinary angelica extract;

[0088] 428.58 grams of common motherwort extract;

[0089] 285.72 grams of common acanthopanax extract;

[0090] 4000 grams of carrier;

[0091] Operate on the raw materials prepared in the above embodiments according to the established process preparation steps.

[0092] Example 4:

[0093] Taking the production of 10 kg of additive as an example, the main raw materials selected are:

[0094] 1121 grams of alfalfa meal;

[0095] 672.6 grams of dandelion extract;

[0096] 807.12 grams of purslane powder;

[0097] 896.8 grams of pine needle powder;

[0098] 538.08 grams of mugwort powder;

[0099] 807.12 grams of astragalus extract;

[0100] 672.6 grams of codonopsis extract;

[0101] 448.4 grams of angelica extract;

[0102] 538.08 grams of motherwort extract;

[0103] 358.72 grams of acanthopanax extract;

[0104] 3138.8 grams of carrier;

[0105] Operate on the raw materials prepared in the above embodiments according to the established process preparation steps.

[0106] Apply the composite additives prepared in Examples 1 to 4 and the composite additives in Comparative Examples 1 and 2 to the laying hen feeding experiment respectively, and operate according to the same laying hen feeding steps.

[0107] By comparing the number of eggs laid, total egg weight / kg, egg breakage, and laying rate of every 100 laying hens raised for 70 days, among which Comparative Example 1 uses the composite additive generated according to the background technology of this article, and Comparative Example 2 uses the common composite additive on the market.

[0108] Table 1: Egg production comparison table of each example and comparative example

[0109] Group Number of eggs laid (pieces) Total egg weight (kg) Number of broken eggs (pieces) Egg production rate (%) Example 1 5800 500 12 93.1 Example 2 5700 480 22 82.7 Example 3 5600 475 25 81.2 Example 4 5500 460 32 78.6 Control Group 1 4800 420 41 61.2 Control Group 2 5000 389 39 60.8

[0110] In summary, the compound additive prepared by the present invention has obvious advantages compared with the common compound additives on the market and the compound additives proposed in the background technology of this article in terms of the number of eggs laid, total egg weight / kg, egg breakage amount, and egg production rate. It can be seen that the number of eggs laid in Examples 1 to 4 are 5,800, 5,700, 5,600, and 5,500 respectively, all higher than 4,800 in Comparative Example 1 and 5,000 in Comparative Example 2. This shows that the compound additive and its preparation method of the present invention help to increase the egg production quantity of laying hens. Due to the optimization of the raw material ratio and preparation process in Example 1, the number of eggs laid is the largest;

[0111] Among them, the total egg weight of Example 1 is 500 kg, that of Example 2 is 480 kg, that of Example 3 is 475 kg, that of Example 4 is 460 kg, while that of Comparative Example 1 is 420 kg and that of Comparative Example 2 is 389 kg. The compound additive of the present invention promotes the laying hens to produce eggs with a greater total weight, reflecting the positive effect of the additive on improving the egg production weight of laying hens. And due to the differences in raw materials and process details in different examples, there is a certain gradient in the total egg weight;

[0112] At the same time, the number of egg breakages in Example 1 is 12, that in Example 2 is 22, that in Example 3 is 25, and that in Example 4 is 32, significantly lower than 41 in Comparative Example 1 and 39 in Comparative Example 2. This shows that the compound additive of the present invention has a positive impact on improving the eggshell quality of eggs, reducing the breakage rate of eggs during production and subsequent processing, and Example 1 has the best effect;

[0113] In addition, the egg production rate of Example 1 reaches 93.1%, that of Example 2 is 82.7%, that of Example 3 is 81.2%, and that of Example 4 is 78.6%, far higher than 61.2% in Comparative Example 1 and 60.8% in Comparative Example 2. This directly reflects that the compound additive of the present invention can effectively improve the egg production rate of laying hens. Example 1 reaches the highest level of egg production rate due to its specific formula and process.

[0114] Details of the comparison effects through feed-to-egg ratio, incidence rate of laying hens, egg yolk color, and Haugh unit are shown in Table 2:

[0115] Table 2: Comparison table of the effects of laying hens consuming compound additives

[0116] Group Feed-to-egg ratio Incidence rate of laying hens (%) Yolk color (Roche color fan) Haugh unit Example 1 2:0:1 3 10 85 Example 2 2:1:1 4 9 83 Example 3 2:1:1 4 9 82 Example 4 2:2:1 5 8 80 Control Group 1 2:5:1 8 7 75 Control Group 2 2:4:1 7 7 76

[0117] Among them, the feed-to-egg ratio represents the amount of feed consumed by laying hens for every 1 kg of eggs produced. The lower the ratio, the higher the feed utilization rate. The feed-to-egg ratios of Examples 1 to 4 are all better than those of the comparative examples, indicating that the compound additive of the present invention helps to improve the feed utilization efficiency. Example 1 has the best effect. The incidence of laying hens reflects the proportion of laying hens getting sick during the breeding period. The incidences of Examples 1 to 4 are significantly lower than those of the comparative examples, indicating that the compound additive enhances the immunity of laying hens and reduces the risk of disease. Example 1 is outstanding in ensuring the health of laying hens. The yolk color can be measured by the Roche color fan. The higher the value, the darker the yolk color, representing better egg quality. The yolk color scores of Examples 1 to 4 are higher than those of the comparative examples, indicating that the additive has a positive effect on improving the yolk color. Example 1 makes the yolk color darker and the quality better. The Haugh unit is used to measure the freshness of eggs and the quality of egg white. The higher the value, the fresher the egg and the better the quality of the egg white.

[0118] The Haugh units of Examples 1 to 4 are higher than those of the comparative examples, reflecting the improvement of the compound additive in the freshness of eggs and the quality of egg white. The eggs in Example 1 have the best freshness and egg white quality.

[0119] Taking the digestive effect of laying hens' gastrointestinal tracts and the content of additive materials in feces as the comparison effect details, see Table 3:

[0120] Table 3: Comparison Table of Digestive Indexes of Laying Hens' Intestines and Digestibility of Additives

[0121]

[0122] Among them, the intestinal villus length: The intestinal villus length is an important indicator reflecting the digestive and absorptive function of laying hens' intestines. Longer intestinal villi can increase the absorption area of the intestine and improve the absorption efficiency of nutrients. The intestinal villus lengths of Examples 1 to 4 are significantly longer than those of the comparative examples, indicating that the compound additive of the present invention helps to promote the growth and development of intestinal villi and improve the intestinal digestive and absorptive function. Example 1 has the most significant effect;

[0123] The activity of digestive enzymes determines the decomposition and digestion ability of laying hens for the nutritional components in feed. The higher the activity, the stronger the digestion ability. The intestinal digestive enzyme activities of Examples 1 to 4 are all higher than those of the comparative examples, indicating that the compound additive can effectively improve the activity of intestinal digestive enzymes and enhance the digestive function of laying hens. Example 1 performs best in enhancing the activity of digestive enzymes;

[0124] The content of undigested additive materials in feces reflects the degree of digestion and utilization of additive materials by laying hens. The lower the content, the better the digestion and absorption of additives by laying hens. The content of undigested additive materials in feces of Examples 1 to 4 is significantly lower than that of the comparative examples, indicating that the compound additive of the present invention is more easily digested and absorbed by laying hens, reducing the waste of materials. Example 1 has the best effect in promoting the digestion and absorption of additives.

[0125] In Example 1 above, the preparation method and raw materials proposed by the present invention were adopted. In Example 2, the raw materials proposed by the present invention and the general formula method were adopted. In Example 3, the preparation method proposed by the present invention and general raw materials were adopted. In Example 4, the raw materials of the present invention (changing the formulation ratio) were adopted. It can be seen from the above four examples and two comparative examples that the innovative steps and formulations improve the effect of the composite additive, which is difficult to compare with general processes and raw materials.

[0126] In terms of the preparation steps of the present invention, the raw material pretreatment controls the flushing flow rate, drying temperature and time, and moisture content, and uses a high-speed pulverizer and a specific sieve to lay a foundation for extraction and improve the extraction rate of active ingredients. Ultrasonic extraction precisely controls the temperature, power, time, and ethanol concentration, and is more efficient than general methods in extracting plant active ingredients, improving the quality of the extract. The probiotic culture precisely controls the conditions and requires the number of viable bacteria to ensure high activity and high quality of the probiotic preparation. In the mixing stage, enteric-coated pellets are added synchronously to achieve targeted release of plant extracts. High-speed stirring promotes coating infiltration, and microcapsule encapsulation precisely controls the release of sensitive ingredients, enhancing the stability and synergistic effect of the composite additive. The temperature is strictly controlled throughout the process, a sterile environment is maintained, and the parameters are intelligently regulated to ensure the stability of product quality and batch uniformity.

[0127] In addition, raw materials such as alfalfa meal and dandelion extract complement each other's advantages. For example, alfalfa meal provides nutrition, and dandelion extract has antibacterial and anti-inflammatory effects, synergistically improving the health and egg-laying performance of laying hens. General processes lack precise control in the aspects of raw material treatment, extraction, and mixing. The general raw material ratio does not consider synergistic effects and cannot give full play to the advantages of each component, resulting in poor effects on improving the egg-laying performance and health level of laying hens.

[0128] All modes of the present invention are within the scope of protection of this patent.

[0129] The embodiments of the present invention are given for the purposes of illustration and description. Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present invention.

Claims

1. A composite additive for enhancing egg production in laying hens, characterized in that: The composition includes the following components in parts by weight: 15-25 parts of alfalfa powder, 8-15 parts of dandelion extract, 10-18 parts of purslane powder, 12-20 parts of pine needle powder, 6-12 parts of wormwood powder, 10-18 parts of astragalus extract, 8-15 parts of codonopsis extract, 5-10 parts of angelica extract, 6-12 parts of motherwort extract, 4-8 parts of Acanthopanax senticosus extract and 50-80 parts of a carrier.

2. The composite additive for enhancing egg production in laying hens according to claim 1, characterized in that: The sum of the weight parts of the alfalfa powder and the dandelion extract is 30-35 parts.

3. The composite additive for enhancing egg production in laying hens according to claim 1, characterized in that: The total weight proportion of the purslane powder, pine needle powder and wormwood powder is 32-40 parts.

4. The composite additive for enhancing egg production in laying hens according to claim 1, characterized in that: The weight ratio of the astragalus extract, codonopsis extract and angelica extract is 1.8-2.2:1.4-1.6:

1.

5. The composite additive for enhancing egg production in laying hens according to claim 1, characterized in that: The carrier is a starch carrier that has been spray-dried and has a water content of less than 5%.

6. The composite additive for enhancing egg production in laying hens according to claim 1, characterized in that: The weight ratio of the Acanthopanax senticosus extract to the carrier is 1:10-1:

12.

7. The composite additive for enhancing egg production in laying hens according to claim 1, characterized in that: The weight proportions of the dandelion extract, the motherwort extract and the Acanthopanax senticosus extract satisfy: dandelion extract - motherwort extract + Acanthopanax senticosus extract = 10-12 parts.

8. A method for preparing a composite additive for enhancing egg production in laying hens as claimed in claims 1 to 7, characterized in that: The following steps are involved: S1: Select specific plant raw materials without mildew and less impurities, rinse with running water for 3-5 times to remove impurities, put into 60-70℃ forced air drying oven, dry for 8-10 hours until the moisture is less than 10%, and then grind into 80-100 mesh with high-speed pulverizer to ensure uniform particles, complete pretreatment, and prepare for subsequent extraction; S2: Put the pre-treated and crushed raw materials into an ultrasonic extraction device, add 8-10 times the amount of 70%-80% ethanol solution by volume, and perform ultrasonic extraction for 30-40 minutes at 40-50°C and 300-400W power. After extraction, concentrate the raw materials at 60-70°C and 0.08-0.09MP vacuum to obtain a concentrated plant extract for standby use; S3: Take an appropriate amount of probiotic preparations such as Bacillus subtilis and Bifidobacterium, inoculate them into a liquid culture medium containing specific nutrients and proportions, adjust the pH to 7.0-7.2, place them in a 37°C constant temperature shaker, and culture them at a speed of 150-200 r / min for 12-16 hours until the number of viable bacteria is not less than 1×10 9 CFU / mL, complete the preparation and set aside; S4: Use an electronic balance to accurately weigh vitamins, minerals, amino acids, etc., and stir at a low speed for 10-15 minutes to premix into a basic mixture. While stirring, add the plant extract concentrate and the specially modified enteric-coated micropellets at the same time; S5: Slowly add the plant extract concentrate to the basic mixture, increase the stirring speed to 300-400r / min, and stir for 20-30 minutes. Use the liquid-solid mixing characteristics to promote encapsulation and infiltration. For easily oxidizable ingredients, use edible biopolymer materials to make microcapsules to encapsulate them, and trigger dissolution and fusion under specific conditions. S6: Add activated probiotic preparation, adjust the stirring speed to 50-100r / min, stir for 15-20 minutes to ensure uniform distribution and no damage, maintain 10-15℃ during the mixing process, use air purification equipment to maintain sterility, and use intelligent control to ensure that the parameters meet the standards to obtain the composite additive.

Images