Radix astragali-blue antitoxic composition, preparation method and traditional Chinese medicine preparation

Abstract

The invention provides a radix astragali and blue antitoxic composition, a preparation method and a traditional Chinese medicine preparation. The astragalus membranaceus and indigowoad leaf antitoxic composition comprises an active component and an absorption enhancer, wherein the active component is prepared from the following components: astragalus membranaceus, indigowoad root, indigowoad leaf, rehmannia, hawthorn, codonopsis pilosula, bighead atractylodes rhizome and chrysanthemum. According to the present invention, the raw material types are subjected to addition and subtraction compatibility, such that the herbs supplement each other, the composition prepared from the 8 drug extracts provides the significant effect in the treatment of the chicken infectious bursal disease, and the absorption enhancer is added to discover that the sodium alginate grafted beta-cyclodextrin can promote the intestinal tract to absorb the active substances so as to provide the significant treatment effect on the chicken infectious bursal disease; the treatment effect is better. The medicine prepared from the composition can quickly stop diarrhea and bleeding, shorten the recovery time of animals and improve the treatment efficiency. In addition, compared with western medicines, the medicine has small side effects on animals, is easy to store, and has better safety and tolerance.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese veterinary medicine, and particularly relates to a Qilan anti-virus composition, a preparation method and a traditional Chinese medicine preparation. Background Art

[0002] Infectious bursal disease (IBD) in chickens, also known as infectious bursitis in chickens, is an infectious disease caused by Infectious Bursal Disease Virus (IBDV). This disease mainly affects poultry, especially young chickens. After being infected, chickens show lethargy, ruffled feathers, and excrete white or yellow loose stools. They may also show lameness or unsteadiness when standing. The growth of the chicken flock is slow, the feed conversion rate is low, the immunity decreases, and it is easy to be secondary to other infections. It is one of the multiple infectious diseases that endanger the chicken farming industry.

[0003] Traditional Chinese medicine believes that when chickens are invaded by external pathogens, wind pathogens, poor ventilation or heavy moisture, the wind pathogens invade the chicken body; damp pathogens, and the functions of the zang-fu organs are disordered; heat pathogens, and heat accumulates in the chicken body, making it prone to inflammation. Qi deficiency, insufficient vital qi, decreased resistance, insufficient yang qi, and decreased immunity. The zang-fu organs are disordered. The spleen is responsible for transporting and transforming water and dampness. When the spleen is weak, water and dampness accumulate internally, leading to dampness accumulation due to spleen deficiency, exuberant liver fire, the liver fire invading the spleen, and resulting in damp-heat pouring downward.

[0004] The drugs disclosed in the prior art for preventing and treating this disease mainly include domestic and imported vaccines, biological products (such as high-titer serum, high-titer egg yolk liquid), western medicines (such as amantadine, moroxydine, etc.) and traditional Chinese medicines (such as Qilan anti-virus drink, cystic disease clear, etc.). However, vaccines, antiserum and egg yolk antibodies are greatly affected by the environment such as preparation, preservation and transportation. Antiserum and egg yolk antibodies often have problems such as uncertain effects. Western anti-viral medicines only relieve the symptoms without treating the root cause. The therapeutic effects of the above preparations are not ideal. Although traditional Chinese medicine can fundamentally regulate the whole body, prevent and control infectious bursal disease in chickens by adjusting the environment, enhancing the physique and adjusting the functions of the zang-fu organs, generally speaking, the effect is slow to take effect and the therapeutic effect cannot be seen immediately.

[0005] Therefore, it is imperative to find a new therapeutic drug from Chinese herbal medicines that has a fast absorption rate, quick effect, can effectively prevent and treat infectious bursal disease in chickens, reduce chicken defecation, and reduce internal bleeding in chickens. Summary of the Invention

[0006] Object of the Invention: The object of the present invention is to provide a Qilan anti-virus composition, a preparation method and a traditional Chinese medicine preparation, so as to solve the problems of slow effect, poor medicinal effect and long recovery time of chickens in the traditional Chinese medicine preparations for treating infectious bursal disease in the existing market.

[0007] Technical Solution of the Present Invention:

[0008] In one aspect, the present invention provides a Qilan anti-virus composition, comprising an active ingredient and an absorption enhancer; the active ingredient is made from the following components:

[0009] Astragalus membranaceus, Isatis indigotica, Folium Isatidis, Rehmannia glutinosa, Crataegus pinnatifida, Codonopsis pilosula, Atractylodes macrocephala, Chrysanthemum morifolium.

[0010] In some embodiments, the active ingredient is made from the following components in parts by weight:

[0011] Astragalus membranaceus 100 - 150, Isatis indigotica 90 - 120, Folium Isatidis 80 - 110, Rehmannia glutinosa 50 - 90, Crataegus pinnatifida 40 - 70, Codonopsis pilosula 50 - 90, Atractylodes macrocephala 40 - 70, Chrysanthemum morifolium 20 - 50.

[0012] Preferably, the active ingredient is made from the following components in parts by weight:

[0013] Astragalus membranaceus 100 - 120, Isatis indigotica 100 - 120, Folium Isatidis 80 - 100, Rehmannia glutinosa 70 - 90, Crataegus pinnatifida 40 - 60, Codonopsis pilosula 70 - 90, Atractylodes macrocephala 40 - 60, Chrysanthemum morifolium 30 - 50.

[0014] More preferably, the active ingredient is made from the following components in parts by weight:

[0015] Astragalus membranaceus 100, Isatis indigotica 100, Folium Isatidis 80, Rehmannia glutinosa 70, Crataegus pinnatifida 40 - 50, Codonopsis pilosula 70 - 80, Atractylodes macrocephala 40 - 50, Chrysanthemum morifolium 30 - 40.

[0016] In some embodiments, the active ingredient is made from the following components in parts by weight: 100 parts by weight of Astragalus membranaceus extract, 100 parts by weight of Isatis indigotica extract, 80 parts by weight of Folium Isatidis extract, 70 parts by weight of Rehmannia glutinosa extract, 40 - 50 parts by weight of Crataegus pinnatifida extract, 70 - 80 parts by weight of Codonopsis pilosula extract, 40 - 50 parts by weight of Atractylodes macrocephala extract, and 30 - 40 parts by weight of Chrysanthemum morifolium extract.

[0017] In some embodiments, the active ingredient is made from the following components in parts by weight: 100 parts by weight of Astragalus membranaceus alcohol extract, 100 parts by weight of Isatis indigotica water extract, 80 parts by weight of Folium Isatidis water extract, 70 parts by weight of Rehmannia glutinosa alcohol extract, 40 - 50 parts by weight of Crataegus pinnatifida alcohol extract, 70 - 80 parts by weight of Codonopsis pilosula alcohol extract, 40 - 50 parts by weight of Atractylodes macrocephala alcohol extract, and 30 - 40 parts by weight of Chrysanthemum morifolium alcohol extract.

[0018] In some embodiments, the extract is prepared by the following method: Wash, disinfect, and dry the raw materials, mix and soak them in a solvent (water, ethanol, or a mixed solution of the two) for 1 - 2 h, and obtain the extract by reduced pressure distillation.

[0019] For example, when the raw material is Astragalus membranaceus, the solvent selected is ethanol: Take Astragalus membranaceus, add 8 - 12 V / W times the amount of the solvent (ethanol), and obtain the Astragalus membranaceus extract by vacuum distillation.

[0020] For example, when the raw material is Isatis indigotica Fort., the solvent selected is water: Take Isatis indigotica Fort., add 8 - 12 V / W times the amount of the solvent (water), and obtain the Isatis indigotica Fort. extract by vacuum distillation.

[0021] Since traditional Chinese medicine is a relatively complex system, the active ingredients in each Chinese herbal medicine are composed of multiple substances, including alkaloids, flavonoids, glycosides, volatile oils, polysaccharides, etc. The chemical components of traditional Chinese medicine are complex and diverse, so the pharmacological effects are also diverse. Therefore, the effects of different chemical substances extracted by water or alcohol are different. There may be components that play a positive role and components that play a negative role. Therefore, it is particularly important to select a suitable solvent.

[0022] In some embodiments, the active ingredient is made of the following components in parts by weight: 100 parts by weight of Astragalus membranaceus alcohol extract, 100 parts by weight of Isatis indigotica Fort. water extract, 80 parts by weight of Isatis tinctoria water extract, 70 parts by weight of Rehmannia glutinosa alcohol extract, 40 - 50 parts by weight of Crataegus pinnatifida alcohol extract, 70 - 80 parts by weight of Codonopsis pilosula alcohol extract, 40 - 50 parts by weight of Atractylodes macrocephala alcohol extract, and 30 - 40 parts by weight of Chrysanthemum morifolium Ramat. alcohol extract.

[0023] In some embodiments, the absorption enhancer is sodium alginate grafted with β - cyclodextrin.

[0024] In some embodiments, the preparation method of the sodium alginate grafted with β - cyclodextrin includes:

[0025] Add 10 - 20 g of β - cyclodextrin into a three - necked flask, successively add 30 - 40 mL of dimethyl sulfoxide and 30 - 40 mL of isopropanol, dropwise add 0.5 - 0.8 mL of epichlorohydrin under stirring, and immediately dropwise add 50 - 100 mL of 0.5 mol / L NaOH solution. Stir at room temperature for 4 - 5 h, then add 2 - 10 g of sodium alginate, continue to stir and react at room temperature for 4 - 5 h, filter, wash until neutral, and dry to obtain sodium alginate grafted with β - cyclodextrin.

[0026] On the other hand, the present application also provides a preparation method of the Qilan anti - virus composition, including:

[0027] S1. Preparation of extracts; Prepare Astragalus membranaceus extract, Isatis indigotica Fort. extract, Isatis tinctoria extract, Rehmannia glutinosa extract, Crataegus pinnatifida extract, Codonopsis pilosula extract, Atractylodes macrocephala extract, and Chrysanthemum morifolium Ramat. extract respectively;

[0028] S2. After cooling the extract, weigh astragalus extract, isatis root extract, folium isatidis extract, rehmannia root extract, hawthorn extract, codonopsis pilosula extract, atractylodes macrocephala extract and chrysanthemum extract according to parts by weight, mix them evenly, inoculate with fermentation strains, seal and ferment at a constant temperature to obtain a fermented bacterial liquid; filter, concentrate and sterilize to obtain an active substance; then add an absorption promoter to obtain the Qilan anti-toxic composition.

[0029] In some embodiments, the fermentation strains are one or more combinations of bacillus subtilis, lactobacillus bulgaricus, and lactobacillus casei; preferably, the fermentation strains are a mixed strain of bacillus subtilis, lactobacillus bulgaricus, and lactobacillus casei, and the mixing weight ratio is 2-10:0.5-4:0.2-1; more preferably, the fermentation strains are a mixed strain of bacillus subtilis, lactobacillus bulgaricus, and lactobacillus casei, and the mixing weight ratio is 5:2:1.

[0030] In some embodiments, the mass ratio of the absorption promoter to the active substance is 2-3:7-8.

[0031] On the other hand, the present invention also provides a traditional Chinese medicine preparation comprising the above-mentioned Qilan anti-toxic composition.

[0032] In some embodiments, the traditional Chinese medicine preparation of the present invention further includes pharmaceutically acceptable excipients: binders (such as microcrystalline cellulose), fillers (such as starch, glucose, anhydrous lactose and lactose beads), disintegrants (such as cross-linked PVP, cross-linked sodium carboxymethyl starch, cross-linked sodium carboxymethyl cellulose, low-substituted hydroxypropyl cellulose), lubricants (such as magnesium stearate), as well as absorption promoters, adsorption carriers, flavoring agents, sweetening agents, excipients, diluents, wetting agents, etc.

[0033] In some embodiments, the traditional Chinese medicine preparation of the present invention can be prepared by conventional methods in the art and can be administered by enteral, parenteral or topical routes; enteral preparations include capsules, tablets, oral liquids, granules, pills, powders, pills, plasters, etc.; parenteral administration preparations include injections, etc.; topical route administration preparations include creams, patches, ointments, sprays, etc. Preferably, it is an enteral preparation.

[0034] Based on the theory of traditional Chinese veterinary medicine and combined with the disease characteristics of infectious bursal disease in chickens, the above formula was designed through syndrome differentiation. Infectious bursal disease in chickens can occur throughout the year, with an incidence rate as high as 74% and a mortality rate up to 64%. Initially affected chickens will peck at their cloacas, reduce food intake, be listless, have ruffled feathers, with their heads and wings drooping; be lethargic, with closed eyelids, stand in a corner, be reluctant to move, have an unsteady gait, lie on the ground, and tremble; have white or watery diarrhea, with soil and feces around the anus, and peck at the anus. Deaths will start to occur successively from the second day after infection, and the peak of deaths generally occurs on the 3rd to 4th day after infection. Chickens that survive the death period will start to recover on the 5th to 7th day. It is one of the multiple infectious diseases that endanger the chicken farming industry. Traditional Chinese veterinarians believe that external pathogens invade the chicken flock, including wind pathogens, poor ventilation or heavy humidity, and the wind pathogens invade the chicken body; damp pathogens, resulting in disharmony of zang-fu functions; heat pathogens, with heat accumulation in the chicken body, making it prone to inflammation. Qi deficiency, insufficient vital energy, decreased resistance, insufficient yang qi, and decreased immunity. Zang-fu disharmony, the spleen is responsible for transporting and transforming water and dampness. When the spleen is weak, water and dampness will accumulate internally, leading to spleen deficiency with excessive dampness, exuberant liver fire, and liver fire invading the spleen, resulting in damp-heat pouring downward. Therefore, from the perspective of traditional Chinese veterinary medicine, the therapeutic principles of strengthening the healthy qi and eliminating pathogenic factors, clearing heat and detoxifying, invigorating the spleen and replenishing qi, and supplementing qi and nourishing blood should be established. So in the formula, astragalus membranaceus, isatis root, folium isatidis are used as the main effective drugs to ensure that the composition of the present invention can clear heat and detoxify, invigorate the spleen and replenish qi, and dispel wind-cold pathogens; rehmannia glutinosa is selected to clear heat and promote fluid production, cool the blood and stop bleeding; codonopsis pilosula which invigorates the spleen and qi, strengthens the healthy qi and consolidates the root, hawthorn which promotes qi circulation, strengthens the stomach and promotes digestion, atractylodes macrocephala which tonifies the lung and qi, strengthens the spleen and dries dampness, and chrysanthemum which clears heat and detoxifies are used as adjuvant drugs to cooperate with the main drugs to clear and eliminate the infectious bronchitis virus in chickens and the remaining pathogenic factors and expel them from the fecal and urinary tracts. Therefore, the present invention has a higher clinical cure rate than related drugs in other existing public technologies, improves the immune function, and enables chickens to recover health faster.

[0035] Beneficial effects:

[0036] Based on the effects of 8 drug components including astragalus membranaceus, isatis root, folium isatidis, rehmannia glutinosa, hawthorn, codonopsis pilosula, atractylodes macrocephala, and chrysanthemum, the present invention reasonably adds and subtracts and combines them, making each drug flavor complement each other. The composition prepared from the extracts of 8 drugs has played an extremely significant effect in preventing and treating infectious bursal disease in chickens. By adding an absorption promoter, it is found that sodium alginate grafted β-cyclodextrin can promote the intestinal absorption of active substances, and the therapeutic effect is better. The drug prepared from this composition can quickly stop diarrhea and bleeding, shorten the recovery time of animals, and improve the treatment efficiency. In addition, this drug has relatively small side effects on animals compared with western medicines, is easy to store, and has better safety and tolerance. Specific implementation manners

[0037] The present invention will be described below in conjunction with specific implementation embodiments. It should be noted that the following examples are examples of the present invention, only for illustrating the present invention, and not for limiting the present invention. Without departing from the gist or scope of the present invention, other combinations and various improvements within the concept of the present invention can be made.

[0038] Unless otherwise specified, the chemical reagents used in the present invention are all ordinary commercially available analytical pure.

[0039] Sources of various traditional Chinese medicines used in the present invention:

[0040] Astragalus membranaceus: sourced from Anhui Xinyuan Traditional Chinese Medicine Technology Co., Ltd., brand Huixinyuan, without sulfur fumigation.

[0041] Isatis indigotica Fort.: sourced from Anhui Xinyuan Traditional Chinese Medicine Technology Co., Ltd., brand Huixinyuan, sulfur-free bulk goods.

[0042] Folium Isatidis: sourced from Anhui Kangweifu Pharmaceutical Co., Ltd.

[0043] Rehmannia glutinosa Libosch.: sourced from Bozhou Mingxutang Pharmaceutical Sales Co., Ltd., raw Rehmannia glutinosa slices.

[0044] Crataegus pinnatifida Bge.: sourced from Anhui Xinyuan Traditional Chinese Medicine Technology Co., Ltd., brand Huixinyuan, naturally sun-dried.

[0045] Codonopsis pilosula (Franch.) Nannf.: sourced from Anhui Xinyuan Traditional Chinese Medicine Technology Co., Ltd., brand Huixinyuan, thick slices.

[0046] Atractylodes macrocephala Koidz.: sourced from Anhui Xinyuan Traditional Chinese Medicine Technology Co., Ltd., brand Huixinyuan, in original form.

[0047] Chrysanthemum morifolium Ramat.: sourced from Anhui Xinyuan Traditional Chinese Medicine Technology Co., Ltd., brand Huixinyuan, Huangshan tribute chrysanthemum.

[0048] Bacillus subtilis and Lactobacillus bulgaricus are sourced from Huiming (Jiaxing) Biotechnology Co., Ltd.; Lactobacillus casei is sourced from Nanjing Bannuo Biotechnology Co., Ltd.

[0049] Preparation of sodium alginate grafted β-cyclodextrin

[0050] Add 10 g of β-cyclodextrin to a three-necked flask, successively add 30 mL of dimethyl sulfoxide and 30 mL of isopropanol, dropwise add 0.6 mL of epichlorohydrin under stirring, and immediately add 50 mL of 0.5 mol / L NaOH solution. Stir at room temperature for 4 h, then add 10 g of sodium alginate, and continue to stir and react at room temperature for 4 h. Filter, wash until neutral, and dry to obtain sodium alginate grafted β-cyclodextrin.

[0051] Examples 1-5 and Comparative Examples 1-5

[0052] S1. Preparation of the extract. Take 1000 g of the material, add a solvent in an amount of 10 times the volume to weight ratio, reflux and extract for 1 hour, extract twice, and obtain an extractive solution by vacuum distillation. The relative density of the extractive solution is 1.0 to obtain the extract. For example, when using astragalus as the material and ethanol as the solvent, the obtained extract is astragalus alcohol extract; when using astragalus as the material and water as the solvent, the obtained extract is astragalus water extract. And so on for other cases.

[0053] S2. After cooling the extractive solution, weigh the extract respectively according to the weight parts in Table 1, mix well, inoculate into a fermentation strain (a mixed strain of Bacillus subtilis, Lactobacillus bulgaricus, and Lactobacillus casei, with a mixed weight ratio of 5:2:1), seal and ferment at a constant temperature to obtain a fermented bacterial liquid; after filtration, concentration, and sterilization, obtain an active substance; then add alginate grafted β-cyclodextrin as an absorption promoter in a mass ratio of absorption promoter: active substance of 2:8, and mix evenly to obtain the Qilan anti-virus composition.

[0054] Table 1 Formulation table of examples

[0055]

[0056]

[0057] Table 2 Formulation table of comparative examples 1-5

[0058] Control Example 1 2 3 4 5 Alcohol extract of Astragalus membranaceus 0 100 100 100 100 Water extract of Astragalus membranaceus 100 0 0 0 0 Water extract of Isatis indigotica Fort 100 100 100 100 100 Water extract of Folium Isatidis 80 0 80 80 80 Alcohol extract of Folium Isatidis 0 80 0 0 0 Alcohol extract of Rehmannia glutinosa 70 70 70 70 70 Alcohol extract of Crataegus pinnatifida Bunge 50 50 0 50 50 Alcohol extract of Codonopsis pilosula 80 80 0 80 80 Alcohol extract of Atractylodes macrocephala Koidz 50 50 50 0 50 Alcohol extract of Chrysanthemum morifolium Ramat 40 40 40 0 0 Alcohol extract of Lonicera japonica Thunb 0 0 0 0 50

[0059] Comparative examples 6-7

[0060] The preparation method of comparative examples 6-7 refers to Example 3, with the only difference being that the fermentation strain is as shown in Table 3.

[0061] Table 3 Fermentation strain table of comparative examples 6-7

[0062] Control Example Fermentation strain species Corresponding added weight ratio Control Example 6 Bacillus subtilis: Lactobacillus bulgaricus: Lactobacillus acidophilus 5：2：1 Control Example 7 Bacillus subtilis: Lactobacillus bulgaricus: Bifidobacterium bifidum 5：2：1

[0063] Comparative examples 8-10

[0064] The preparation method of comparative examples 8-10 refers to Example 3, with the only difference being that the absorption promoter is as shown in Table 4.

[0065] Table 4 Table of comparative examples 8-10

[0066]

[0067]

[0068] Conduct the following tests on the obtained Qilan anti-virus composition:

[0069] 1. Pharmacological test

[0070] Experimental animals: 260 broilers clinically diagnosed with infectious French bursal disease; before the experiment, they were raised as usual, fed with complete chick feed without any drugs, and had free access to food and water.

[0071] Test method: Broilers diagnosed with infectious French bursal disease were taken as clinical trial subjects and randomly divided into 1-13 groups, which were respectively Example 1 Group (D1), Example 2 Group (D2), Example 3 Group (D3), Example 4 Group (D4), Example 5 Group (D5), Comparative Example 1 Group (L1), Comparative Example 2 Group (L2), Comparative Example 3 Group (L3), Comparative Example 4 Group (L4), Comparative Example 5 Group (L5), Comparative Example 6 Group (L6), Comparative Example 7 Group (L7), Comparative Example 8 Group (L8), Comparative Example 9 Group (L9), Comparative Example 10 Group (L10) and blank control group (no medication group). The Qilan anti-toxic composition used for treatment was fed with 1000L of water per L throughout the day for 3 consecutive days. Free access to food and drinking water. After administration, the clinical symptoms and cure of the diseased chickens in each group were observed, and the cure amount, mortality amount and symptom duration were statistically calculated. The test results are shown in Table 4.

[0072] If chickens die during the test, the autopsy of the dead chickens shows characteristic lesions of the bursa of Fabricius, such as severe inflammation, bleeding, necrosis, and atrophy of the bursa of Fabricius. In addition, the dead chickens are dehydrated, and the leg and chest muscles often bleed, and the color is dark red. The kidneys are swollen, and the renal tubules and ureters are filled with white urate. The spleen and the mucosa at the junction of the glandular stomach and the gizzard are bleeding, which is considered to be infected death. All are calculated into the mortality. When the chickens do not have white or yellow loose stools, lameness or unstable standing, pecking at the anus, etc., and eat and sleep normally, the symptoms are considered to have disappeared.

[0073] Table 4 Test results

[0074]

[0075]

[0076] Note: “The shortest time for symptom disappearance” refers to the shortest time taken by a sample of test animals from the start of the test to complete recovery in each test group.

[0077] Judging from the number of deaths, the number of cured cases, and the shortest duration of symptoms, the therapeutic effect of the Qilan anti-virus composition of the present invention has made certain progress. The mortality rate of the experimental group has been greatly reduced, and complete cure can be achieved. The curative effect of the administration sample in Example 3 is relatively better, and the cure rate for chicken infectious bursal disease can reach 100%, and the shortest treatment time can be shortened to 3 days. Among them, when the weight ratio of the raw materials is: astragalus membranaceus 100-120, isatis root 100-120, folium isatidis 80-100, rehmannia glutinosa 70-90, hawthorn 40-60, codonopsis pilosula 70-90, atractylodes macrocephala 40-60, and chrysanthemum 30-50, the medicinal effect is the best.

[0078] As can be seen from the examples of the present invention, traditional Chinese medicine, as a complex system, has different effects for the chemical substances extracted by different solvents. It can be seen from the comparative examples that the astragalus membranaceus extract and folium isatidis extract obtained by water extraction or alcohol extraction have a certain impact on the therapeutic effect of the final Qilan anti-virus composition product, that is, there is a synergistic effect. And through multiple experiments, the inventor confirmed that the combination of astragalus membranaceus alcohol extract, isatis root water extract, folium isatidis water extract, rehmannia glutinosa alcohol extract, hawthorn alcohol extract, codonopsis pilosula alcohol extract, atractylodes macrocephala alcohol extract and chrysanthemum alcohol extract has the best effect, and sodium alginate grafted β-cyclodextrin can effectively promote the intestinal absorption of active substances in traditional Chinese medicine, shorten the treatment time, and has technological progress.

[0079] The present invention can also have many other embodiments. Without departing from the spirit and essence of the present invention, those skilled in the art can make various corresponding changes and deformations according to the present invention, but these corresponding changes and deformations should all fall within the protection scope of the appended claims of the present invention.

Claims

1. A stilbene blue anti-toxic composition, comprising an active ingredient and an absorption promoter; characterized in that: The active ingredient is made from the following components: Astragalus, Isatis Root, Isatis Leaf, Rehmannia Glutinosa, Crataegus Pinellia, Codonopsis Pilosula, Atractylodes Macrocephala, Chrysanthemum.

2. The stilbene blue anti-toxic composition according to claim 1, characterized in that: The active ingredient is made from the following components in parts by weight: Astragalus 100-150, Isatis root 90-120, Isatis indigotica 80-110, Rehmannia glutinosa 50-90, Hawthorn 40-70, Codonopsis pilosula 50-90, Atractylodes macrocephala 40-70, Chrysanthemum 20-50.

3. The stilbene blue anti-toxic composition according to claim 1, characterized in that: The active ingredient is made of the following components in parts by weight: 100 parts by weight of astragalus extract, 100 parts by weight of isatis root extract, 80 parts by weight of isatis leaf extract, 70 parts by weight of rehmannia root extract, 40-50 parts by weight of hawthorn extract, 70-80 parts by weight of codonopsis pilosula extract, 40-50 parts by weight of atractylodes macrocephala extract and 30-40 parts by weight of chrysanthemum extract.

4. The stilbene blue anti-toxic composition according to claim 3, characterized in that: The active ingredient is prepared from the following components in parts by weight: 100 parts by weight of astragalus alcohol extract, 100 parts by weight of isatis root water extract, 80 parts by weight of isatis indigotica water extract, 70 parts by weight of rehmannia glutinosa alcohol extract, 40-50 parts by weight of hawthorn alcohol extract, 70-80 parts by weight of codonopsis pilosula alcohol extract, 40-50 parts by weight of atractylodes macrocephala alcohol extract and 30-40 parts by weight of chrysanthemum alcohol extract.

5. The stilbene blue anti-toxic composition according to claim 1, characterized in that: The absorption promoter is sodium alginate grafted with beta-cyclodextrin.

6. The method for preparing the stilbene blue anti-toxic composition according to any one of claims 1 to 5, characterized in that: include: S1. Preparation of extracts; preparing astragalus extract, isatis root extract, isatis leaf extract, rehmannia extract, hawthorn extract, codonopsis extract, atractylodes extract and chrysanthemum extract respectively; S2. After the extract is cooled, weigh the astragalus extract, isatis root extract, isatis indigotica extract, rehmannia extract, hawthorn extract, codonopsis extract, atractylodes extract and chrysanthemum extract according to weight proportions, mix well, inoculate into the fermentation strain, seal and ferment at a constant temperature to obtain a fermentation solution; filter, concentrate and sterilize to obtain the active substance; then add an absorption enhancer to obtain the astragalus and isatis anti-toxic composition.

7. The method for preparing the stilbene blue anti-toxic composition according to claim 6, characterized in that: The fermentation strain is one or more combinations of Bacillus subtilis, Lactobacillus bulgaricus and Lactobacillus casei.

8. A Chinese medicine preparation, characterized in that: A stilbene antitoxic composition comprising the stilbene blue antitoxic composition according to any one of claims 1 to 5.

9. The Chinese medicine preparation according to claim 8, characterized in that: The traditional Chinese medicine preparation also includes pharmaceutically acceptable excipients.

10. The Chinese medicine preparation according to claim 8, characterized in that: The Chinese medicine preparation can be administered enterally, parenterally or topically.

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