Traditional Chinese medicine composition for treating heat stress injury

Abstract

The invention discloses a traditional Chinese medicine composition, which is prepared from the following raw materials: cortex fraxini, herba portulacae, artemisia rupestris, duckweed, rhizoma atractylodis macrocephalae, poria cocos and liquorice in a weight ratio of (15-20): (15-20): (15-20): (15-20): (7-12): (7-12): 10, and the traditional Chinese medicine composition is a mixture obtained by mixing screen underflow obtained after the medicinal materials are crushed and sieved by a 80-mesh sieve. The traditional Chinese medicine composition can improve the average daily gain of heat stress poultry animals; the feed conversion ratio of heat stress poultry animals is reduced; the body temperature of the heat stress poultry animals is reduced; the respiratory rate of heat stress poultry animals is reduced; treating or alleviating intestinal injury of heat stress poultry animals; the content of IL-10 in serum of poultry animals is increased; and the content of IL-1beta, IL-6 and TNF-alpha in serum of poultry animals can be reduced. The traditional Chinese medicine composition has an application prospect.

Description

Technical Field

[0001] The present invention belongs to the field of traditional Chinese medicine and relates to a traditional Chinese medicine composition for treating heat stress injury. Background Art

[0002] In modern livestock and poultry farming, broiler chickens are heat-sensitive poultry. Heat stress is a relatively harmful stress for broiler chickens with a relatively fast growth rate. Broiler stress syndrome is also an important common epidemic disease in poultry. According to the biological habits of broiler chickens, 10 - 30°C is the basic environment in which they can maintain their body temperature, and 21 - 26°C is the suitable environmental temperature range for broiler chickens. Within this range, broiler chickens can exhibit better growth performance. When the temperature rises to 26 - 30°C, although broiler chickens are in an uncomfortable state, they can still maintain normal physiological functions. However, when the temperature is higher than this range, it will cause disorders in the physiological functions of chickens and reduce the production performance of broiler chickens. There is no sweat gland distribution on the body surface of broiler chickens, and they are covered with feathers, so they are easily damaged by heat stress. When the environmental temperature exceeds 30°C, for every 1°C increase, the feed intake of broiler chickens decreases by 4.6%, which quickly leads to a decline in production performance and causes significant economic losses. The number of chicken deaths caused by heat stress and the indirect economic losses caused by it around the world every year are extremely huge.

[0003] The impact of heat stress on the broiler chicken body is multi-faceted. Existing research has shown that heat stress can change the physiological and biochemical indexes of broiler chicken blood, such as increasing blood glucose concentration and stress-related hormone levels, etc. Heat stress can also lead to abnormal immune function in broiler chickens, manifested as thymocyte necrosis and a decrease in antibody levels in serum, and induce diseases in multiple organs such as the liver, kidney, and brain. In recent years, heat stress-induced intestinal injury has gradually become a research hotspot in this field. The mechanism of heat stress on intestinal injury is particularly complex. Research has shown that under heat stress, the blood supply to the intestinal tract of livestock and poultry is insufficient, most nutrient transport proteins are inhibited, and the utilization rate of oxygen and nutrients is significantly reduced, inducing intestinal oxidative stress and inflammation, and then leading to damage to the intestinal barrier. The damage of various intestinal barriers makes pathogenic microorganisms and other harmful substances in the intestinal tract easily invade the submucosa, causing local pathological changes in the intestine and even triggering a systemic inflammatory response, further exacerbating the damage of heat stress to broiler chickens.

[0004] At present, in modern poultry farming, measures to relieve heat stress in broiler chickens include reducing the density of the chicken flock, implementing physical cooling in the chicken house, adding vitamins and minerals to drinking water, etc. Adding compound traditional Chinese medicine to the diet has been proven to effectively relieve heat stress in poultry, which may be related to aspects such as reducing stress hormone levels, enhancing the body's immunity, and alleviating intestinal injury. In recent years, using traditional Chinese medicine as a feed additive to resist heat stress in broiler chickens has gradually become a research hotspot in this field.

[0005] Qin Pi (Latin name: Fraxini Cortex) is the dried branch bark or stem bark of Fraxinus chinensis Roxb., Fraxinus rhynchophylla Hance, Fraxinus stylosa Lingelsh. or Fraxinus szaboana Lingelsh. of the Oleaceae family. It is bitter, astringent, and cold in nature. It has the effects of astringing to stop diarrhea, clearing heat and drying dampness, arresting vaginal discharge, and improving eyesight. It is mostly used for the treatment of heat-toxic dysentery, red and swollen painful eyes, corneal nebula, and leukorrhea with redness.

[0006] Portulaca oleracea L. is an annual herb, glabrous throughout. It is sour and cold. It enters the heart, liver, spleen, and large intestine meridians. It has the effects of treating heat-toxic dysentery, erysipelas, carbuncles, and scrofula. Modern pharmacological research shows that this substance can improve the elasticity of blood vessel walls, which is beneficial for preventing and treating cardiovascular diseases, and also has the effect of preventing enteritis.

[0007] Agastache rugosa is the whole herb of Agastache rugosa of the Lamiaceae family, a perennial herb. It is pungent and slightly warm. It enters the spleen, stomach, and lung meridians. It has the pharmacological effects of expelling pathogenic factors from the exterior, removing dampness stagnation, aromatically resolving dampness, and regulating the middle energizer to stop pain. It is mainly used for the related syndromes of less food intake, abdominal distension, and lassitude caused by the spleen being trapped by dampness, internal obstruction of damp turbidity, and disordered transportation and transformation. Modern pharmacological experiments prove that its aromatic smell has the effect of increasing gastric juice secretion and then promoting digestion.

[0008] Lemna minor L. is a perennial floating plant of the Araceae family. It is pungent and cold. It enters the lung and small intestine meridians. It has the effects of clearing heat and detoxifying, reducing swelling and relieving pain. It is mostly used for epidemic febrile diseases and has a certain antipyretic effect.

[0009] Atractylodes macrocephala Koidz is a perennial herb of the genus Atractylodes of the Asteraceae family. It is pungent and slightly warm, and enters the spleen and stomach meridians. It has the functions of strengthening the spleen and stomach, drying dampness and promoting diuresis, stopping sweating, and preventing miscarriage.

[0010] Poria cocos is the dried sclerotium of the fungus Poria cocos of the Polyporaceae family. It is sweet, light, and neutral in nature. When used as medicine, it has the functions of benefiting the spleen and stomach, promoting diuresis to eliminate dampness, and calming the mind. Modern medical research shows that Poria cocos can enhance the immune function of the body.

[0011] Glycyrrhizae Radix et Rhizoma is a perennial herb with thick roots and rhizomes. It is neutral in nature and sweet in taste, and enters the twelve meridians. It has the effects of clearing heat and detoxifying, moistening the lungs and relieving cough, and coordinating various herbs. It is mainly used to treat cough, sore throat, weakness of the spleen and stomach, hepatitis, gastric and duodenal ulcers, hysteria, carbuncles and boils, etc.

[0012] There are no reports on the treatment of avian heat stress injury with the above seven medicinal materials, and even less on the combined use of the above seven medicinal materials to treat avian heat stress injury. Summary of the Invention

[0013] Aiming at the deficiencies existing in the existing Chinese herbal medicine prevention and control technology for broiler heat stress, the present invention aims to provide a compound Chinese herbal medicine formula for anti-broiler heat stress and the test data in its actual application. By selecting excellent medicinal materials and exploring their ratios, and formulating the preparation method when forming the formula, it ultimately achieves safe and effective alleviation of the reduction in production performance and intestinal damage caused by broiler heat stress.

[0014] The first aspect of the present invention provides a composition, and the raw materials of the composition are from Cortex Fraxini, Portulaca oleracea L., Artemisia scoparia Waldst. et Kit., Lemna minor L., Atractylodes macrocephala Koidz., Poria cocos (Schw.) Wolf, and Glycyrrhiza uralensis Fisch.

[0015] In some embodiments, the weight ratio of the amounts of Cortex Fraxini, Portulaca oleracea L., Artemisia scoparia Waldst. et Kit., Lemna minor L., Atractylodes macrocephala Koidz., Poria cocos (Schw.) Wolf, and Glycyrrhiza uralensis Fisch. in the raw materials of the composition is 2 - 6:2 - 6:2 - 6:2 - 6:1 - 3:1 - 3:5.

[0016] In some embodiments, the weight ratio of the amounts of Cortex Fraxini, Portulaca oleracea L., Artemisia scoparia Waldst. et Kit., Lemna minor L., Atractylodes macrocephala Koidz., Poria cocos (Schw.) Wolf, and Glycyrrhiza uralensis Fisch. in the raw materials of the composition is 15 - 20:15 - 20:15 - 20:15 - 20:7 - 12:7 - 12:10.

[0017] In some embodiments, the weight ratio of the amounts of Cortex Fraxini, Portulaca oleracea L., Artemisia scoparia Waldst. et Kit., Lemna minor L., Atractylodes macrocephala Koidz., Poria cocos (Schw.) Wolf, and Glycyrrhiza uralensis Fisch. in the raw materials of the composition is one of the following R1, R2, R3, or R4:

[0018] R1: 3:3:4:4:2:2:2;

[0020] R2: 4:4:3:3:2:2:2;

[0022] R3: 18:18:18:18:9:9:10;

[0024] R4:

[0025] 3:3:3:3:2:3:3.

[0026] In some embodiments, the Cortex Fraxini is selected from the dried branch bark of Fraxinus chinensis Roxb.; and / or

[0027] the Portulaca oleracea L. is selected from the dried above-ground part of the plant; and / or

[0028] the Artemisia scoparia Waldst. et Kit. is selected from the dried above-ground part of the plant; and / or

[0029] the Lemna minor L. is selected from the dried whole herb; and / or

[0030] the Atractylodes macrocephala Koidz. is selected from the dried rhizome; and / or

[0031] The Poria cocos is selected from dried sclerotium; and / or

[0032] The licorice is selected from dried roots and rhizomes.

[0033] In some embodiments, the composition further contains pharmaceutically acceptable excipients; and / or

[0034] The dosage form of the composition is powder, pill, tablet, elixir, decoction.

[0035] The second aspect of the present invention provides a preparation method of the composition described in the first aspect of the present invention. Mix the Cortex Fraxini, the Portulaca oleracea, the Artemisia scoparia, the Lemna minor, the Atractylodes macrocephala, the Poria cocos, and the licorice and then pulverize them to obtain the composition; or

[0036] Pulverize the Cortex Fraxini, the Portulaca oleracea, the Artemisia scoparia, the Lemna minor, the Atractylodes macrocephala, the Poria cocos, and the licorice separately and then mix them to obtain the composition.

[0037] In some embodiments, in the composition, the particles of all materials can pass through the sieve holes of an 80-mesh sieve.

[0038] The third aspect of the present invention provides the use of the composition described in the first aspect of the present invention in the preparation of a preparation for improving the health status of vertebrates;

[0039] The improvement of the health status of vertebrates includes:

[0040] Increasing the average daily gain of heat-stressed vertebrates;

[0041] Reducing the feed-to-meat ratio of heat-stressed vertebrates;

[0042] Reducing the body temperature of heat-stressed vertebrates;

[0043] Reducing the respiratory rate of heat-stressed vertebrates;

[0044] Treating or alleviating intestinal injuries of heat-stressed vertebrates;

[0045] Increasing the content of IL-10 in the serum of vertebrates; and

[0046] Reducing the contents of IL-1β, IL-6 and TNF-α in the serum of vertebrates.

[0047] In some embodiments, the vertebrates are avian animals such as chickens, ducks, geese, pigeons, quails, turkeys, etc.

[0048] Compared with the prior art, the advantages of the present invention are:

[0049] 1. The compound Chinese herbal medicine formula for anti-heat stress in broiler chickens provided by the present invention conforms to the new concept of current green and healthy breeding, and is a safe, drug residue-free, and antibiotic-reducing Chinese medicine formula product;

[0050] 2. The compound Chinese herbal medicine formula for anti-heat stress in broiler chickens provided by the present invention shows the characteristics of quick effect, stable effect, small dosage, and low price after being applied in actual production, and is a Chinese herbal medicine formula product with great potential for application and promotion. Description of the Drawings

[0051] Figure 1 It is the statistical result of broiler chicken body temperature detection.

[0052] Figure 2 It is the statistical result of broiler chicken respiratory rate detection.

[0053] Figure 3 It is the detection result of HE staining of broiler chicken ileum (100×).

[0054] Figure 4 It is the statistical result of the ratio of broiler chicken ileum villus height / crypt depth detection.

[0055] Figure 5 It is the detection result of HE staining of broiler chicken ileum (400×).

[0056] Figure 6 It is the statistical result of the change in the content of DAO in broiler chicken serum.

[0057] Figure 7 It is the statistical result of the change in the content of cytokines in broiler chicken serum. Detailed Embodiments

[0058] To make the objectives, technical solutions, and advantages of the present invention clearer, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0059] The detailed information of the Chinese medicinal materials used in the present invention is as follows:

[0060] (1) Cortex Fraxini, the place of origin is Shaanxi, the part is the dried branch bark of Fraxinus chinensis Roxb., and the primary processing method is to take it out after moistening thoroughly, cut it into filaments, and dry it.

[0061] (2) Portulaca oleracea L., the place of origin is Heilongjiang, the part is the dried above-ground part, and the primary processing method is to wash it, dry the water, steam it, and then dry it in the sun.

[0062] (3) Elsholtzia ciliata (Thunb.) Hyland., the place of origin is Heilongjiang, the part is the dried above-ground part, and the primary processing method is to moisten it thoroughly, cut it into short sections, and dry it in the sun.

[0063] (4) Spirodela polyrhiza (L.) Schleid., the place of origin is Heilongjiang, the part is the whole plant, and the primary processing method is to dry it in the sun.

[0064] (5) Atractylodes macrocephala Koidz., produced in Zhejiang, part is the dried rhizome, and the primary processing method is to wash with water, moisten thoroughly, cut into thick slices, and dry.

[0065] (6) Poria cocos (Schw.) Wolf., produced in Sichuan, part is the dried sclerotium, and the primary processing method is to slice after airing in the shade with ventilation.

[0066] (7) Glycyrrhiza uralensis Fisch., produced in Gansu, part is the dried root and rhizome, and the primary processing method is to cut off the stem base, young buds, lateral roots and fibrous roots, wash, moisten thoroughly, cut into thick slices, and dry.

[0067] The basic requirements of the technical solution of the present invention for each Chinese medicinal material are as follows: the moisture content of each component is less than 10%, and the content of other impurities should not be higher than 0.5%.

[0068] Example 1: Preparation of Composition 1 (Test Group 1)

[0069] A compound Chinese herbal medicine composition for resisting heat stress in broiler chickens is made from the following Chinese medicinal material raw materials by weight percentage: Cortex Fraxini 15%, Portulaca oleracea L. 15%, Artemisia scoparia Waldst. et Kit. 20%, Spirodela polyrhiza Schleid. 20%, Atractylodes macrocephala Koidz. 10%, Poria cocos (Schw.) Wolf. 10%, Glycyrrhiza uralensis Fisch. 10%.

[0070] The above 7 kinds of Chinese medicinal materials are respectively crushed by a pulverizer, passed through an 80-mesh sieve, and the materials passing through the sieve are mixed evenly according to the weight ratio required above to obtain Composition 1, which is sealed and reserved for use.

[0071] Example 2: Preparation of Composition 2 (Test Group 2)

[0072] A compound Chinese herbal medicine composition for resisting heat stress in broiler chickens is made from the following Chinese medicinal material raw materials by weight percentage: Cortex Fraxini 20%, Portulaca oleracea L. 20%, Artemisia scoparia Waldst. et Kit. 15%, Spirodela polyrhiza Schleid. 15%, Atractylodes macrocephala Koidz. 10%, Poria cocos (Schw.) Wolf. 10%, Glycyrrhiza uralensis Fisch. 10%.

[0073] The above 7 kinds of Chinese medicinal materials are respectively crushed by a pulverizer, passed through an 80-mesh sieve, and the materials passing through the sieve are mixed evenly according to the weight ratio required above to obtain Composition 2, which is sealed and reserved for use.

[0074] Example 3: Preparation of Composition 3 (Test Group 3)

[0075] A compound Chinese herbal medicine composition for resisting heat stress in broiler chickens is made from the following Chinese medicinal material raw materials by weight percentage: Cortex Fraxini 18%, Portulaca oleracea L. 18%, Artemisia scoparia Waldst. et Kit. 18%, Spirodela polyrhiza Schleid. 18%, Atractylodes macrocephala Koidz. 9%, Poria cocos (Schw.) Wolf. 9%, Glycyrrhiza uralensis Fisch. 10%.

[0076] The above 7 kinds of Chinese medicinal materials are respectively crushed by a pulverizer, passed through an 80-mesh sieve, and the materials passing through the sieve are mixed evenly according to the weight ratio required above to obtain Composition 3, which is sealed and reserved for use.

[0077] Example 4: Preparation of Composition 4 (Test Group 4)

[0078] A compound Chinese herbal medicine composition for resisting heat stress in broiler chickens is made from the following Chinese herbal medicine raw materials by weight percentage: 15% of Cortex Fraxini, 15% of Portulaca oleracea L., 15% of Artemisia scoparia Waldst. et Kit., 15% of Lemna minor L., 10% of Atractylodes macrocephala Koidz., 15% of Poria cocos (Schw.) Wolf, and 15% of Glycyrrhiza uralensis Fisch.

[0079] The above 7 Chinese herbal medicines are respectively pulverized by a pulverizer, passed through an 80-mesh sieve, and the materials under the sieve are taken and mixed evenly according to the weight ratio required above to obtain Composition 4, which is sealed for later use.

[0080] Example 5: Clinical efficacy test

[0081] In order to study the drug activities of four compositions, the present invention conducts a clinical efficacy test. In a certain broiler chicken farm in Harbin, 600 21-day-old white broiler chickens (half male and half female, randomly grouped) are selected. Among them, 400 broiler chickens in the experimental group are divided into 4 groups, with 100 in each group, 100 in the stress group, and 100 in the blank group. They are raised in chicken coops in the same building but different rooms. The blank group is raised in a constant-temperature chicken coop with the temperature set at 22 ± 2°C. The other 5 groups of broiler chickens are all raised in a temperature-controlled chicken coop. From 10:00 to 18:00 every day, a heat stress treatment (36 ± 1°C) is carried out for 8 h with a warm air blower, and the temperature is 22 ± 2°C at other times. Free ventilation is provided, and they are raised in a stepped cage, with free drinking water and artificial feeding of feed. The blank group and the stress group are fed a basal diet, and the other 4 groups are respectively added with one of the four compositions of the present invention at 1 w / w% in the basal diet. They are fed 2 times a day, and each feeding is until the feed in the trough is eaten up. If there is any remaining, it is cleaned up and included in the uneaten feed. The feed fed every day is responsible by a special person, and the feed consumption record is made on the same day. The humidity of the two chicken coops is controlled between 50 - 60%. The basal diet is formulated with reference to NRC (1994), and the composition and nutritional level of the basal diet are shown in Table 1. The test period is from 21 to 42 days old, for a total of 21 d.

[0082] Table 1. Formulation and nutritional level of the basal diet

[0083]

[0084] Premix 1 contains the following nutrients (dosage per kilogram of diet): vitamin A, 10000 IU; vitamin D3, 3000 IU; vitamin B1, 2 mg; vitamin B2, 6 mg; niacin, 50 mg; vitamin E, 50 mg; vitamin K3, 2 mg; biotin, 0.1 mg; folic acid, 1 mg; pantothenic acid, 12 mg; choline, 500 mg; iron (ferrous sulfate) 80 mg, copper (copper sulfate) 8 mg, zinc (zinc sulfate) 60 mg, manganese (manganese sulfate) 80 mg, iodine (potassium iodide) 0.5 mg, selenium (sodium selenite) 0.2 mg.

[0085] Starting from 21 days of age, the body weight was measured and recorded at 8:00 every day, and the feed was fed at 8:00 and 18:00 every day. The daily feeding amount and the remaining amount were recorded. Feed was cut off at 20:00 on the night before 42 days of age, and the chickens were weighed after 12 hours of fasting. During the experiment, about 10 chickens were randomly selected from each group at about 16:00 every three days, and a veterinary electronic thermometer was used to detect the rectal temperature. At the same time, the respiratory rate was monitored. At the end of the 42-day experiment, 10 chickens with body weights close to the average value were randomly selected from each treatment (1 rooster was taken from each of replicates 1, 3, 5, 7, and 9; 1 hen was taken from each of replicates 2, 4, 6, 8, and 10). Blood was collected from the wing vein, and 2 tubes were collected. One tube was used for the determination of whole blood physiological and biochemical indexes, and the other tube was used to separate serum. The separated serum was used for the determination of cytokines such as IL-10, IL-1β, IL-6, and TNF-α (using an enzyme-linked immunosorbent assay kit purchased from Jiangsu Jingmei Biotechnology Co., Ltd.) and the detection of intestinal permeability (diamine oxidase DAO kit purchased from Nanjing Jiancheng Bioengineering Institute). After slaughter, the ileum was separated, and a 4-cm intestinal segment was collected and fixed with 10% formalin solution for detecting the intestinal morphological structure.

[0086] The collected ileum tissues were fixed with 4% paraformaldehyde for 24 h, and then dehydrated, cleared, infiltrated with wax, embedded, sectioned, mounted on slides, baked, and then subjected to HE staining. The steps were as follows: dewaxing and rehydrating with xylene; staining with hematoxylin for 30 min, and then rinsing with tap water for 60 s; staining with eosin for 10 s for slight coloring, and finally rinsing with purified water for 60 s; putting into 95% ethanol I and 95% ethanol II for 1 min each, 100% ethanol for 2 min, and then putting into xylene I and xylene II for 5 min each. After complete dehydration, the cover glass was removed, 2 drops of neutral gum were added to the slide, and the cover glass was covered. Then, it could be observed and photographed under an optical microscope. The villus height (VH) and crypt depth (CD) were measured and recorded using an image analysis software (CaseViewer), and the villus height / crypt depth (V / C) was calculated.

[0087] Observation of intestinal ultrastructure: The intestinal tissues that had been fixed with 2.5% glutaraldehyde for 2 h were taken out, and on a clean surface, they were cut into tissue blocks with a length of 2 mm and a width of 3 mm using a blade to avoid damaging the intestinal villi; then rinsed 3 times with PBS, 15 min each time. Fixed with 1% osmium tetroxide in the dark for 2 h; rinsed repeatedly with PBS 3 times again. Immersed in ethanol with concentrations of 30%, 50%, 70%, 80%, 90%, and 95% for dehydration for 15 min each; dehydrated with 100% ethanol 2 times, 15 min each time; put into acetone for replacement for 15 min; transferred from acetone to isoamyl acetate for 15 min; the samples were taken out and transferred to the sample chamber of a critical point dryer. After reaching the critical state (31 °C, 72.8 atmospheres), the temperature was raised by 10 °C to vaporize liquid carbon dioxide and dry the tissue blocks. After sputter coating with gold using an ion sputtering instrument, they were observed and photographed under a scanning electron microscope.

[0088] (1) Test results of the effects of 4 kinds of compositions on the production performance of broilers

[0089] From 21 days old, the body weight was measured and recorded at 8:00 every day, and the feeding amount and the remaining amount were recorded at 8:00 and 18:00 every day. The average feed intake of broilers decreased extremely significantly after heat stress (P<0.01). The average feed intakes of the 4 experimental groups were all increased compared with the stress group, and there was a significant difference between experimental group 3 and the stress group (P<0.01). The average daily gain of broilers also decreased significantly after heat stress (P<0.01). The average daily gains of broilers in the 4 experimental groups were all increased. There was a significant difference between experimental group 3 and experimental group 4 and the stress group (P<0.05). Among the 4 experimental groups, there was a significant difference between experimental group 3 and the other 3 groups (P<0.05). After calculating the feed to meat ratio, it was found that the feed to meat ratio of broilers increased significantly after heat stress (P<0.01), while the feed to meat ratios of the 4 experimental groups were all significantly decreased compared with the stress group (P<0.01). Through comprehensive analysis, it can be seen that the effect of experimental group 3 is better, which can improve the average feed intake and daily gain of broilers and reduce the feed to meat ratio.

[0090] Table 2. Statistical table of the production performance of broilers

[0091]

[0092] Note: ** represents extremely significant difference compared with the blank group (P<0.01), * represents significant difference compared with the blank group (P<0.05); ## represents extremely significant difference compared with the stress group (P<0.01), # represents significant difference compared with the stress group (P<0.05); && represents extremely significant difference compared with experimental group 3 (P<0.01), & represents significant difference compared with experimental group 3 (P<0.05).

[0093] (2) Test results of the effects of 4 kinds of formulas on the body temperature and respiration of broilers

[0094] During the test period, about 10 chickens were randomly selected from each group at about 16:00 every 3 days, and a veterinary electronic thermometer was used to detect the rectal temperature, and at the same time the respiration rate was monitored. The detection results of the respiration and body temperature of broilers showed (see Figure 1 、 Figure 2 ), compared with the blank group, the respiration rates and body temperatures of broilers in each group increased extremely significantly (P<0.01); compared with the stress group, the differences in the body temperature drops of broilers in experimental group 3 and experimental group 4 were extremely significant (P<0.01), and the respiration rates of broilers in experimental group 1, experimental group 2, experimental group 3 and experimental group 4 decreased (P<0.01). It can be seen from this that experimental group 3 and experimental group 4 can reduce the respiration rate and body temperature of broilers.

[0095] Figure 1 and Figure 2 Among them, ** indicates extremely significant difference compared with the blank group (P<0.01), * indicates significant difference compared with the blank group (P<0.05); ## indicates extremely significant difference compared with the stress group (P<0.01), # indicates significant difference compared with the stress group (P<0.05).

[0096] (3) Test results of the effects of 4 formulas on the intestinal structure of broilers

[0097] HE staining results ( Figure 3 ) showed that the ileum tissue structure of the blank group was clear, the villi were arranged neatly, and no obvious lesions were seen. Compared with the blank group, the intestinal villi of the stress group were partially broken. After the intervention of the 4 formulas, the intestinal damage was alleviated to varying degrees. The measurement results of the ratio of intestinal villus height to crypt depth ( Figure 4 ) showed that compared with the blank group, the ratio of intestinal villus height / crypt depth in the stress group, experimental group 1 and experimental group 4 decreased, with extremely significant differences (P<0.01). Compared with the stress group, the ratio of intestinal villus height / crypt depth in the 4 experimental groups of broilers increased, among which the differences in experimental group 2 and experimental group 3 were extremely significant (P<0.01), and the differences in experimental group 1 and experimental group 4 were significant (P<0.05). Among the 4 experimental groups, the ratio of intestinal villus height / crypt depth in experimental group 3 was the largest, and there were significant differences compared with experimental group 1 and experimental group 4 (P<0.05). This indicates that all 4 groups of formulas can relieve the intestinal damage caused by heat stress, and the effect of experimental group 3 is better.

[0098] Figure 4 Among them, ** indicates extremely significant difference compared with the blank group (P<0.01), * indicates significant difference compared with the blank group (P<0.05); ## indicates extremely significant difference compared with the stress group (P<0.01), # indicates significant difference compared with the stress group (P<0.05); && indicates extremely significant difference compared with experimental group 3 (P<0.01), & indicates significant difference compared with experimental group 3 (P<0.05).

[0099] It can be seen from Figure 5 that the intestinal villi of the blank group were arranged neatly and the surface was smooth, while the intestinal villi of the stress group were arranged disorderly, the surface was rough and incomplete, rough, like burrs, and some villi were broken. After the intervention of the 4 formulas, the intestinal villi damage was alleviated to varying degrees. Among them, the alleviating effect of the intestinal villi in experimental group 3 was better, and the surface of the intestinal villi was relatively smooth.

[0100] (4) Test results of the effects of 4 formulas on the intestinal function of broilers

[0101] The activity of diamine oxidase (DAO) in blood was detected using a DAO test kit (rate method) and a microplate reader. This indicator can reflect the integrity of the small intestinal mucosal barrier function and the degree of intestinal mucosal cell damage. When the intestinal mucosal barrier function is damaged or intestinal epithelial cells are necrotic, the serum DAO level increases, making it a sensitive biomarker for intestinal injury.

[0102] The test results of the DAO content in serum were obtained from Figure 6 It can be seen that: compared with the blank group, the DAO level in the stress group increased extremely significantly (P < 0.01). Compared with the stress group, the DAO levels in all 4 experimental groups decreased extremely significantly. Among them, there were significant differences between experimental group 3 and experimental groups 1 and 4 (P < 0.05). This indicates that experimental group 3 had the best effect and could effectively alleviate intestinal mucosal dysfunction.

[0103] Figure 6 In, ** represents extremely significant difference compared with the blank group (P < 0.01), * represents significant difference compared with the blank group (P < 0.05); ## represents extremely significant difference compared with the stress group (P < 0.01), # represents significant difference compared with the stress group (P < 0.05); && represents extremely significant difference compared with experimental group 3 (P < 0.01), & represents significant difference compared with experimental group 3 (P < 0.05).

[0104] Based on the above results, it can be seen that all 4 formulations can alleviate heat stress injury to a certain extent. However, through comparison, it was found that experimental group 3 could reduce the body temperature and respiratory rate of broilers caused by heat stress and improve the production performance of broilers. At the same time, experimental group 3 could restore the intestinal structure and function, thereby alleviating intestinal injury.

[0105] (V) Test results of the effects of 4 formulations on cytokines in broiler serum

[0106] The content of cytokines in serum was detected using the enzyme-linked immunosorbent assay. As sensitive inflammatory indicators, cytokines are important detection indicators for precise treatment and rational drug use. Under stress conditions, the body undergoes an inflammatory response, resulting in an increase in pro-inflammatory factors and a decrease in anti-inflammatory factors.

[0107] The test results of the cytokine content in serum were obtained from Figure 7It can be seen that: compared with the blank group, the content of IL-10 in the stress group decreased extremely significantly (P<0.01), while the contents of IL-1β, IL-6, and TNF-α increased extremely significantly (P<0.01). Compared with the stress group, the content of IL-10 in all 4 experimental groups increased extremely significantly, and the contents of IL-1β, IL-6, and TNF-α decreased significantly (P<0.05). There was a significant difference in TNF-α between experimental group 3 and experimental group 4 (P<0.05), and there was an extremely significant difference in IL-1β between experimental groups 2 and 3 and the stress group (P<0.01).

[0108] Figure 7 In the figure, ** indicates an extremely significant difference compared with the blank group (P<0.01), * indicates a significant difference compared with the blank group (P<0.05); ## indicates an extremely significant difference compared with the stress group (P<0.01), # indicates a significant difference compared with the stress group (P<0.05); && indicates an extremely significant difference compared with experimental group 3 (P<0.01), & indicates a significant difference compared with experimental group 3 (P<0.05).

[0109] As is known by common technical knowledge, the present invention can be implemented by other embodiments that do not depart from its spiritual essence or essential features. Therefore, the above-disclosed embodiments are illustrative in all aspects and not exclusive. All changes within the scope of the present invention or equivalent to the present invention are encompassed by the present invention.

Claims

1. A composition, the raw materials of which are from Cortex Fraxini, Portulaca Oleracea, Artemisia selengensis, Duckweed, Atractylodes macrocephala, Poria cocos and Licorice.

2. The composition according to claim 1, characterized in that The weight ratio of the raw materials of the composition, including cortex qinpi, purslane, artemisia selengensis, duckweed, atractylodes macrocephala, tuckahoe and liquorice, is 2-6:2-6:2-6:2-6:1-3:1-3:

5.

3. The composition according to claim 1 or 2, characterized in that The weight ratio of the raw materials of the composition, including cortex qinpi, purslane, artemisia selengensis, duckweed, atractylodes macrocephala, tuckahoe and liquorice, is 15-20:15-20:15-20:15-20:7-12:7-12:

10.

4. The composition according to any one of claims 1 to 3, characterized in that The weight ratio of the raw materials of the composition, including Cortex Fraxini, Portulaca Oleracea, Artemisia selengensis, Duckweed, Atractylodes macrocephala, Poria cocos and Licorice, is as follows: R1, R2, R3 or R4: R1： 3：3：4：4：2：2：2； R2： 4：4：3：3：2：2：2； R3： 18：18：18：18：9：9：10； R4： 3：3：3：3：2：3：3。 5. The composition according to claim 1, characterized in that The fraseri is selected from the dried bark of Fraxinus chinensis; and / or The purslane is selected from the dried aerial parts of the plant; and / or The Artemisia selengensis is selected from the dried aerial parts of the plant; and / or The duckweed is selected from dried whole grass; and / or The Atractylodes macrocephala is selected from dried rhizomes; and / or The Poria cocos is selected from dried sclerotia; and / or The licorice is selected from dried roots and rhizomes.

6. The composition according to claim 1, characterized in that The composition further contains pharmaceutically acceptable excipients; and / or The dosage form of the composition is powder, pill, tablet, pill or decoction.

7. The method for preparing the composition according to any one of claims 1 to 5, characterized in that: The cortex qinpi, the purslane, the artemisia selengensis, the duckweed, the atractylodes macrocephala, the poria cocos, and the liquorice are mixed and crushed to obtain the composition; or The cortex qinpi, the herb purslane, the herb wormwood, the duckweed, the herb atractylodes, the herb tuckahoe, and the herb licorice are ground separately and then mixed to obtain the composition.

8. The preparation method according to claim 7, characterized in that: In the composition, particles of all materials can pass through the openings of an 80-mesh sieve.

9. Use of the composition according to any one of claims 1 to 6 in the preparation of a preparation for improving the health of vertebrates; Improving the health of vertebrates includes: Improve the average daily weight gain of heat-stressed vertebrates; Reducing the feed-to-meat ratio of heat-stressed vertebrates; Reduces body temperature in heat-stressed vertebrates; Reduces respiratory rate in heat-stressed vertebrates; Treat or alleviate intestinal damage in heat-stressed vertebrates; Increase the level of IL-10 in vertebrate serum; and Reduce the levels of IL-1β, IL-6 and TNF-α in vertebrate serum.

10. The use according to claim 9, characterized in that The vertebrates are poultry animals such as chickens, ducks, geese, pigeons, quails, turkeys, etc.

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