Traditional Chinese medicine for enhancing immunity and treating pain symptoms and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine for enhancing immunity and treating pain symptoms. The traditional Chinese medicine is prepared from the following raw materials: musk, flos carthami, radix notoginseng, cortex cinnamomi, herba lysimachiae, radix achyranthis bidentatae, cortex eucommiae, rhizoma seu radix notopterygii, fructus gardeniae, fossil fragments, poria cocos, radix codonopsis, radix glycyrrhizae preparata, radix paeoniae alba, pericarpium citri reticulatae, radix angelicae sinensis, rhizoma polygoni cuspidati, radix polygonati officinalis, bulbus lilii, lygodium japonicum, semen persicae, radix rehmanniae, fructus jujubae, lactobacillus rhamnosus freeze-dried powder and lactobacillus casei freeze-dried powder. Through reasonable compatibility of the raw materials and control of the dosage, the obtained traditional Chinese medicine has the effects of rapidly relieving pain and playing a role for a long time, has extremely strong effects of relieving pain, promoting blood circulation to remove blood stasis, clearing and activating the channels and collaterals, strengthening the body resistance to eliminate pathogenic factors and enhancing immunity, can effectively regulate body functions, treats both symptoms and root causes and is not easy to relapse. A small amount of lactobacillus rhamnosus and lactobacillus casei are added, and the lactobacillus rhamnosus and the lactobacillus casei can cooperate with other raw materials, so that the intestinal flora structure of a human body is effectively adjusted, the immunity of the human body is favorably enhanced, the immunosuppression state of the body is improved, and the immune function of the body is enhanced.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and more specifically to a traditional Chinese medicine for enhancing immunity and treating pain symptoms and its preparation method. Background Art

[0002] With the development of modern society, pain in muscles and bones has become a common disease among the general population. Due to various bad habits of people, such as sitting in front of a computer for a long time, lying on a sofa or soft cushion for a long time to brush mobile phones, lack of exercise, incorrect postures during daily work, and sports injuries, etc., it will cause pain in various muscles and bones of the body. However, since the symptoms of the above-mentioned pain discomfort are not obvious in the initial stage of onset, people will ignore such problems, which will lead to long-term accumulation, resulting in increased pain in the later stage, and then evolving into various muscle and bone inflammations or diseases.

[0003] Currently, when treating pain caused by various diseases, Western medicine is often the main treatment. However, on the one hand, the symptomatic range of Western medicine is narrow, and the side effects are relatively large. After taking it, many adverse reactions are likely to occur, increasing the burden on patients; on the other hand, drug tolerance problems will occur after taking painkillers for a long time or in large doses, making the pain relief effect not ideal. At the same time, patients with long-term pain often have a decline in immunity, and the intestinal flora ecosystem is damaged, resulting in poor digestion and absorption function, weakness and weight loss, and it is easy to cause various complications, further threatening the health of patients.

[0004] Therefore, how to provide a traditional Chinese medicine for enhancing immunity and treating pain symptoms and its preparation method is an urgent problem to be solved by those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention provides a traditional Chinese medicine for enhancing immunity and treating pain symptoms and its preparation method.

[0006] In order to achieve the above object, the present invention adopts the following technical scheme:

[0007] A traditional Chinese medicine for enhancing immunity and treating pain symptoms, comprising the following raw materials: moschus, safflower, notoginseng, cinnamon, lysimachia christinae, achyranthes bidentata, eucommia ulmoides, notopterygium incisum, gardenia jasminoides, dragon bone, poria cocos, codonopsis pilosula, roasted licorice root, white peony root, dried tangerine peel, angelica sinensis, polygonum cuspidatum, polygonatum odoratum, lilium brownii, lygodium japonicum, peach kernel, rehmannia glutinosa, Chinese date, lactobacillus rhamnosus, lactobacillus casei.

[0008] Preferably, by weight, it includes the following raw materials: 60-80 parts of moschus, 60-80 parts of safflower, 45-60 parts of notoginseng, 45-55 parts of cinnamon, 40-55 parts of lysimachia christinae, 40-55 parts of achyranthes bidentata, 40-50 parts of eucommia ulmoides, 40-50 parts of notopterygium incisum, 40-50 parts of gardenia jasminoides, 40-50 parts of fossil fragments, 30-35 parts of poria cocos, 30-35 parts of codonopsis pilosula, 30-35 parts of honey-fried licorice root, 20-30 parts of white peony root, 20-30 parts of dried tangerine peel, 20-30 parts of angelica sinensis, 10-15 parts of polygonum cuspidatum, 10-15 parts of polygonatum odoratum, 10-15 parts of lilium brownii, 10-15 parts of lygodium japonicum, 5-8 parts of peach kernel, 5-8 parts of rehmannia glutinosa, 5-8 parts of jujube, 0.5-5 parts of freeze-dried powder of lactobacillus rhamnosus, 0.5-5 parts of freeze-dried powder of lactobacillus casei.

[0009] Preferably, by weight, it includes the following raw materials: 70 parts of moschus, 70 parts of safflower, 50 parts of notoginseng, 50 parts of cinnamon, 50 parts of lysimachia christinae, 50 parts of achyranthes bidentata, 45 parts of eucommia ulmoides, 45 parts of notopterygium incisum, 45 parts of gardenia jasminoides, 45 parts of fossil fragments, 33 parts of poria cocos, 33 parts of codonopsis pilosula, 33 parts of honey-fried licorice root, 25 parts of white peony root, 25 parts of dried tangerine peel, 25 parts of angelica sinensis, 12 parts of polygonum cuspidatum, 12 parts of polygonatum odoratum, 12 parts of lilium brownii, 12 parts of lygodium japonicum, 6 parts of peach kernel, 6 parts of rehmannia glutinosa, 6 parts of jujube, 1 part of freeze-dried powder of lactobacillus rhamnosus, 1 part of freeze-dried powder of lactobacillus casei.

[0010] Preferably, in the freeze-dried powder, the viable count of lactobacillus rhamnosus is 10 8 ~10 9 CFU / g, and the viable count of lactobacillus casei is 10 8 ~10 9 CFU / g.

[0011] As an invention concept identical to the above technical solution, the present invention also claims to protect the preparation method of the above traditional Chinese medicine, including the following steps:

[0012] (1) Weigh the raw materials according to the above weight parts and set aside;

[0013] (2) Crush moschus, safflower, notoginseng, cinnamon, lysimachia christinae, achyranthes bidentata, eucommia ulmoides, notopterygium incisum, gardenia jasminoides, fossil fragments, poria cocos, codonopsis pilosula, honey-fried licorice root, white peony root, dried tangerine peel, and angelica sinensis, pass through an 80-100 mesh sieve, heat and reflux extract with deionized water 2-4 times, filter, combine the extraction solutions, and concentrate to obtain extraction solution I;

[0014] (3) Crush polygonum cuspidatum, polygonatum odoratum, lilium brownii, lygodium japonicum, peach kernel, rehmannia glutinosa, and jujube, pass through an 80-100 mesh sieve, add them to an ethanol aqueous solution, carry out microwave-assisted heating and reflux extraction 2-4 times, filter, combine the extraction solutions, and concentrate to obtain extraction solution II;

[0015] (4) Mix the extract I with the extract II, perform ultrasonic treatment, and then concentrate it into a thick paste. After cooling, add the freeze-dried powder of Lactobacillus rhamnosus and the freeze-dried powder of Lactobacillus casei, mix and stir, then perform vacuum drying, pulverize, granulate, and fill into capsules to obtain the product.

[0016] Preferably, in step (2), the heating temperature is 91 - 97 °C, the liquid-solid ratio is 8 - 12:1, and the extraction time for each time is 30 - 90 min.

[0017] Preferably, in step (3), the volume concentration of the ethanol aqueous solution is 70% - 90%, the heating temperature is 65 - 75 °C, the extraction time for each time is 30 - 60 min, and the power of the microwave is 300 - 800 W.

[0018] Preferably, in step (4), the power of the ultrasonic is 500 - 800 W, the frequency is 1.5 - 2 MHz, and the time is 30 - 40 min.

[0019] Preferably, in step (4), the relative density of the thick paste is 1.2 - 1.25.

[0020] Preferably, in step (4), the rotation speed of the stirring is 300 - 500 r / min, and the time is 60 - 120 min.

[0021] As an invention concept identical to the above technical solution, the present invention also claims the use of the above traditional Chinese medicine, characterized in that the use is for preparing a drug for enhancing immunity and treating pain.

[0022] Through the above technical solution, compared with the prior art, the present invention provides a traditional Chinese medicine for enhancing immunity and treating pain symptoms and its preparation method, including the following beneficial effects:

[0023] (1) Through the reasonable compatibility of raw materials and the control of dosage in the present invention, the obtained traditional Chinese medicine has the effects of rapid pain relief and long-term and persistent action, has extremely strong analgesic, promoting blood circulation to remove blood stasis, dredging channels and collaterals, strengthening healthy qi to eliminate pathogenic factors, and enhancing immunity effects, can effectively regulate the body functions, treat both the symptoms and the root causes, and is not easy to relapse.

[0024] (2) The present invention adds a small amount of Lactobacillus rhamnosus and Lactobacillus casei, and the two can act synergistically with the remaining raw materials to effectively regulate the intestinal flora structure of the human body, help enhance the immunity of the human body, improve the immune suppression state of the body, and enhance the immune function of the body.

[0025] (3) The preparation method of the present invention can effectively extract the active ingredients of the traditional Chinese medicine, maximize the role of the raw materials, each active ingredient plays different effects respectively, and a synergistic effect is generated among various effects, enhancing the pain relief effect.

[0026] (4) Compared with Western painkillers, the traditional Chinese medicine components of the present invention are mild, have no side effects, and have a low treatment cost. Detailed implementation manners

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0028] Moschus: It is warm in nature and pungent in taste, and belongs to the heart and spleen meridians. It has the functions of promoting blood circulation to remove blood stasis and eliminating swelling, and can relieve pain symptoms by regulating the activities of the nervous system, reducing pain conduction and pain response.

[0029] Carthamus tinctorius: It is pungent in taste and warm in nature, and belongs to the heart and liver meridians. It has the effects of promoting blood circulation to dredge the meridian and dispelling stasis to relieve pain.

[0030] Panax notoginseng: It is sweet and slightly bitter in taste, and warm in nature. It has the functions of removing stasis to stop bleeding and activating blood circulation to relieve pain.

[0031] Cinnamomum cassia: Its medicinal properties are pungent and sweet, and it belongs to the kidney, spleen, heart and liver meridians. Cinnamomum cassia contains cinnamaldehyde, which has anti-excitation and analgesic effects. Since cinnamaldehyde has the function of acting on the central nervous system, it realizes sedative analgesia by inhibiting the activity of neurons.

[0032] Lysimachia christinae: It is slightly cold in nature, sweet and salty in taste, and belongs to the liver, gallbladder, kidney and bladder meridians. It can relieve symptoms such as dysmenorrhea, lower abdominal pain, rheumatic pain, bone pain, stomach pain, etc., and has a good analgesic effect.

[0033] Achyranthes bidentata: Its medicinal properties are bitter, sweet, sour and flat. It belongs to the liver and kidney meridians. It has the function of promoting blood circulation to remove blood stasis, can improve local blood circulation, relieve pain, disperse swelling and dissipate nodules, relieve local swelling and inflammation, promote tissue repair, and strengthen tendons and bones.

[0034] Eucommia ulmoides: Its taste is sweet and warm, and it belongs to the liver and kidney meridians. It can strengthen tendons and bones and activate blood circulation to relieve pain. It contains various active ingredients such as flavonoids, which can improve the body's hypoxia tolerance and improve the body's immune function.

[0035] Notopterygium incisum: It is pungent and bitter in taste, warm in nature, and belongs to the bladder and kidney meridians. It can dispel wind and dampness, relieve pain, promote blood circulation, enhance immunity, and promote the recovery of joint function.

[0036] Gardenia jasminoides: It is bitter and cold in taste, and belongs to the heart, lung and triple energizer meridians. It can cool blood and detoxify, reduce swelling and relieve pain. Ursolic acid is one of the effective components of Gardenia jasminoides, and it has obvious hypothermic and sedative effects.

[0037] Dragon's Bone: Sweet, astringent, neutral in nature, entering the heart, liver, and kidney meridians. Dragon's Bone has the effects of clearing heat and detoxifying, reducing swelling and alleviating pain. It contains a variety of immune-active substances, which can effectively remove toxins from the body, resist the invasion of viruses and bacteria, and improve the body's immunity and anti-fatigue ability.

[0038] Poria: Mild in nature, sweet and bland in taste, entering the heart, lung, spleen, and kidney meridians. It contains a variety of alkaloids and polysaccharide substances, which can regulate the immune system and enhance human immunity.

[0039] Codonopsis pilosula: Sweet, neutral in nature, entering the spleen and lung meridians. It has an anti-inflammatory effect, can reduce the inflammatory response, and relieve pain and swelling caused by inflammation.

[0040] Prepared Licorice Root: Sweet, neutral in nature, entering the heart and spleen meridians. It relieves pain, calms the mind, and can effectively relieve various types of pain, including muscle soreness, joint pain, headache, and toothache.

[0041] White Peony Root: Slightly cold in nature, bitter and sour in taste. Entering the liver and spleen meridians, it has the effects of promoting blood circulation to relieve pain, regulating immunity, and protecting against liver damage.

[0042] Tangerine Peel: Pungent, bitter, warm in nature. Entering the spleen and lung meridians. It regulates qi and strengthens the spleen, dries dampness and resolves phlegm, promotes qi circulation to relieve pain. Multiple active ingredients in tangerine peel can regulate the body's immune function and improve resistance.

[0043] Angelica sinensis: Sweetish in taste, pungent, slightly warm in nature, entering the liver, heart, and spleen meridians. It enriches blood and promotes blood circulation, regulates menstruation and relieves pain.

[0044] Japanese Knotweed: Sour, bitter, cold in nature, entering the liver and gallbladder meridians. It dispels stasis and relieves pain, promotes blood circulation to reduce swelling.

[0045] Polygonatum odoratum: Sweet, cold in nature. Entering the lung and stomach meridians. It nourishes yin and moistens the lung, nourishes the stomach and promotes the production of body fluid, promotes blood circulation in the human body, and regulates the secretion of immune factors to maintain immune balance.

[0046] Lily: Sweet, slightly cold in nature, entering the heart and lung meridians. It clears the heart and calms the mind, promotes qi circulation to relieve pain, and improves immunity.

[0047] Spora Lygodii: Sweet, cold in nature, entering the bladder and small intestine meridians. It nourishes yin and replenishes blood, strengthens the stomach and nourishes the liver, stops bleeding and relieves pain, dissipates stasis and reduces swelling.

[0048] Peach Kernel: Neutral in nature, bitter and sweet in taste, entering the heart, liver, and large intestine meridians. It promotes blood circulation to remove stasis, has anti-inflammatory and analgesic effects.

[0049] Rehmannia glutinosa: Cold in nature, sweet and bitter in taste, entering the heart, liver, and kidney meridians. It reduces swelling and alleviates pain, promotes blood circulation to remove stasis, regulates immune function, and enhances human immunity.

[0050] Jujube: Warm in nature, sweet in taste, entering the spleen, stomach, and heart meridians. It can promote the generation of white blood cells, reduce serum cholesterol, increase serum albumin, and enhance the body's immunity.

[0051] The freeze-dried powder of Lactobacillus rhamnosus used in the present invention was purchased from Beina Chuanglian Biotechnology Co., Ltd. in the mall, with the number BNCC186192 and the viable count of 10 9 CFU / g. The freeze-dried powder of Lactobacillus casei used in the present invention was purchased from Beina Chuanglian Biotechnology Co., Ltd. in the mall, with the number BNCC186562 and the viable count of 10 9 CFU / g.

[0052] Example 1

[0053] A traditional Chinese medicine for enhancing immunity and treating pain symptoms, by weight, comprises the following raw materials: 60 parts of moschus, 60 parts of safflower, 45 parts of notoginseng, 45 parts of cinnamon, 40 parts of lysimachia christinae, 40 parts of achyranthes bidentata, 40 parts of eucommia ulmoides, 40 parts of notopterygium incisum, 40 parts of gardenia jasminoides, 40 parts of dragon bone, 30 parts of poria cocos, 30 parts of codonopsis pilosula, 30 parts of roasted licorice root, 20 parts of white peony root, 20 parts of dried tangerine peel, 20 parts of angelica sinensis, 10 parts of polygonum cuspidatum, 10 parts of polygonatum odoratum, 10 parts of lilium brownii, 10 parts of lygodium japonicum, 5 parts of peach kernel, 5 parts of rehmannia glutinosa, 5 parts of jujube, 0.5 part of freeze-dried powder of Lactobacillus rhamnosus, 0.5 part of freeze-dried powder of Lactobacillus casei;

[0054] The preparation method of the above traditional Chinese medicine comprises the following steps:

[0055] (1) Weigh the raw materials according to the above weight parts and set aside;

[0056] (2) Crush moschus, safflower, notoginseng, cinnamon, lysimachia christinae, achyranthes bidentata, eucommia ulmoides, notopterygium incisum, gardenia jasminoides, dragon bone, poria cocos, codonopsis pilosula, roasted licorice root, white peony root, dried tangerine peel, angelica sinensis, pass through an 80-mesh sieve, extract twice by heating under reflux with deionized water, filter, combine the extracts, and concentrate to obtain Extract I;

[0057] (3) Crush polygonum cuspidatum, polygonatum odoratum, lilium brownii, lygodium japonicum, peach kernel, rehmannia glutinosa, jujube, pass through an 80-mesh sieve, add to an ethanol aqueous solution, and perform microwave-assisted heating under reflux extraction twice, filter, combine the extracts, and concentrate to obtain Extract II;

[0058] (4) Mix Extract I and Extract II, perform ultrasonic treatment, then concentrate into a thick paste, cool, add the freeze-dried powder of Lactobacillus rhamnosus and the freeze-dried powder of Lactobacillus casei, mix and stir, then perform vacuum drying, pulverize, granulate, and fill into capsules to obtain;

[0059] The temperature of the heating in step (2) is 91 °C, the liquid-solid ratio is 8:1, and the extraction time for each time is 30 min;

[0060] The volume concentration of the ethanol aqueous solution in step (3) is 70%, the heating temperature is 65 °C, the extraction time for each time is 30 min, and the power of the microwave is 300 W;

[0061] The power of the ultrasound in step (4) is 500 W, the frequency is 1.5 MHz, and the time is 30 min;

[0062] Preferably, the relative density of the thick paste in step (4) is 1.2.

[0063] Preferably, the stirring speed in step (4) is 300 r / min and the stirring time is 60 min.

[0064] Example 2

[0065] A Chinese medicine for enhancing immunity and treating pain symptoms, comprising the following raw materials in parts by weight: 80 parts of musk, 80 parts of safflower, 60 parts of Panax notoginseng, 55 parts of cinnamon bark, 55 parts of Lysimachia chinensis, 55 parts of Achyranthes bidentata, 50 parts of Eucommia bark, 50 parts of Notopterygium wilfordii, 50 parts of Gardenia jasminoides, 50 parts of Dragon bone, 35 parts of Poria cocos, 35 parts of Codonopsis pilosula, 35 parts of Radix Glycyrrhizae Preparata, 30 parts of White Peony Root, 30 parts of Tangerine Peel, 30 parts of Angelica sinensis, 15 parts of Polygonatum cuspidatum, 15 parts of Polygonatum odoratum, 15 parts of Lilium odoratum, 15 parts of Lygodium japonicum, 8 parts of Prunus persica, 8 parts of Rehmannia glutinosa, 8 parts of jujube, 5 parts of Lactobacillus rhamnosus freeze-dried powder, and 5 parts of Lactobacillus casei freeze-dried powder;

[0066] The preparation method of the above-mentioned Chinese medicine comprises the following steps:

[0067] (1) Weigh the raw materials according to the above weight parts and set aside;

[0068] (2) grinding musk, safflower, Panax notoginseng, cinnamon bark, Lysimachia chinensis, Achyranthes bidentata, Eucommia bark, Notopterygium wilfordii, Gardenia jasminoides, Dragon bone, Poria cocos, Codonopsis pilosula, Radix glycyrrhizae, white peony root, dried orange peel, and Angelica sinensis, passing through a 100-mesh sieve, and extracting with deionized water for 4 times by heating and refluxing, filtering, combining the extracts, and concentrating to obtain an extract I;

[0069] (3) Grind Polygonum cuspidatum, Polygonatum odoratum, Lilium sibiricum, Lygodium japonicum, Prunus persica, Rehmannia glutinosa, and jujube, pass through a 100-mesh sieve, add to an ethanol aqueous solution, perform microwave-assisted heating reflux extraction 4 times, filter, combine the extracts, and concentrate to obtain extract II;

[0070] (4) Mixing the extract I and the extract II, ultrasonically treating, and then concentrating them into a thick paste, adding the freeze-dried powder of Lactobacillus rhamnosus and the freeze-dried powder of Lactobacillus casei after cooling, mixing and stirring, and then vacuum drying, crushing, granulating, and encapsulating to obtain;

[0071] The heating temperature in step (2) is 97° C., the liquid-to-solid ratio is 12:1, and the extraction time is 90 min each time;

[0072] The volume concentration of the ethanol aqueous solution in step (3) is 90%, the heating temperature is 75° C., the extraction time is 60 min each time, and the microwave power is 800 W;

[0073] The power of the ultrasound in step (4) is 800 W, the frequency is 2 MHz, and the time is 40 min;

[0074] The relative density of the thick paste in step (4) is 1.25;

[0075] The stirring speed in step (4) is 500 r / min and the stirring time is 120 min.

[0076] Example 3

[0077] A Chinese medicine for enhancing immunity and treating pain symptoms, comprising the following raw materials in parts by weight: 70 parts of musk, 70 parts of safflower, 50 parts of Panax notoginseng, 50 parts of cinnamon bark, 50 parts of Lysimachia chinensis, 50 parts of Achyranthes bidentata, 45 parts of Eucommia bark, 45 parts of Notopterygium wilfordii, 45 parts of Gardenia jasminoides, 45 parts of Dragon bone, 33 parts of Poria cocos, 33 parts of Codonopsis pilosula, 33 parts of Radix Glycyrrhizae Preparata, 25 parts of White Peony Root, 25 parts of Tangerine Peel, 25 parts of Angelica sinensis, 12 parts of Polygonatum cuspidatum, 12 parts of Polygonatum odoratum, 12 parts of Lilium odoratum, 12 parts of Lygodium japonicum, 6 parts of Peach Kernel, 6 parts of Rehmannia glutinosa, 6 parts of Jujube, 1 part of Lactobacillus rhamnosus freeze-dried powder, and 1 part of Lactobacillus casei freeze-dried powder;

[0078] The preparation method of the above-mentioned Chinese medicine comprises the following steps:

[0079] (1) Weigh the raw materials according to the above weight parts and set aside;

[0080] (2) grinding musk, safflower, Panax notoginseng, cinnamon bark, Lysimachia chinensis, Achyranthes bidentata, Eucommia bark, Notopterygium wilfordii, Gardenia jasminoides, Dragon bone, Poria cocos, Codonopsis pilosula, Radix glycyrrhizae, white peony root, dried orange peel, and Angelica sinensis, passing through a 100-mesh sieve, and extracting with deionized water for three times by heating and refluxing, filtering, combining the extracts, and concentrating to obtain an extract I;

[0081] (3) Grind Polygonum cuspidatum, Polygonatum odoratum, Lilium sibiricum, Lygodium japonicum, Prunus persica, Rehmannia glutinosa, and jujube, pass through a 100-mesh sieve, add to an ethanol aqueous solution, perform microwave-assisted heating reflux extraction three times, filter, combine the extracts, and concentrate to obtain extract II;

[0082] (4) Mixing the extract I and the extract II, ultrasonically treating, and then concentrating them into a thick paste, adding the freeze-dried powder of Lactobacillus rhamnosus and the freeze-dried powder of Lactobacillus casei after cooling, mixing and stirring, and then vacuum drying, crushing, granulating, and encapsulating to obtain;

[0083] The heating temperature in step (2) is 95° C., the liquid-to-solid ratio is 10:1, and the extraction time is 60 min each time;

[0084] The volume concentration of the ethanol aqueous solution in step (3) is 80%, the heating temperature is 70° C., the extraction time is 40 min each time, and the microwave power is 500 W;

[0085] In step (4), the power of the ultrasonic wave is 600 W, the frequency is 1.5 MHz, and the time is 30 min;

[0086] In step (4), the relative density of the thick paste is 1.2;

[0087] In step (4), the rotation speed of the stirring is 300 r / min, and the time is 60 min.

[0088] Comparative Example 1

[0089] A traditional Chinese medicine, calculated by weight parts, comprises the following raw materials: 140 parts of safflower, 50 parts of notoginseng, 50 parts of cinnamon, 50 parts of lysimachia christinae, 50 parts of achyranthes bidentata, 45 parts of eucommia ulmoides, 45 parts of notopterygium incisum, 45 parts of gardenia jasminoides, 45 parts of fossil fragments, 33 parts of poria cocos, 33 parts of codonopsis pilosula, 33 parts of roasted licorice root, 25 parts of white peony root, 25 parts of dried tangerine peel, 25 parts of angelica sinensis, 12 parts of polygonum cuspidatum, 12 parts of polygonatum odoratum, 12 parts of lilium brownii, 12 parts of lygodium japonicum, 6 parts of peach kernel, 6 parts of rehmannia glutinosa, 6 parts of Chinese date, 1 part of freeze-dried powder of lactobacillus rhamnosus, 1 part of freeze-dried powder of lactobacillus casei;

[0090] The preparation method of the traditional Chinese medicine is the same as that of Example 3.

[0091] Comparative Example 2

[0092] A traditional Chinese medicine, calculated by weight parts, comprises the following raw materials: 140 parts of moschus, 50 parts of notoginseng, 50 parts of cinnamon, 50 parts of lysimachia christinae, 50 parts of achyranthes bidentata, 45 parts of eucommia ulmoides, 45 parts of notopterygium incisum, 45 parts of gardenia jasminoides, 45 parts of fossil fragments, 33 parts of poria cocos, 33 parts of codonopsis pilosula, 33 parts of roasted licorice root, 25 parts of white peony root, 25 parts of dried tangerine peel, 25 parts of angelica sinensis, 12 parts of polygonum cuspidatum, 12 parts of polygonatum odoratum, 12 parts of lilium brownii, 12 parts of lygodium japonicum, 6 parts of peach kernel, 6 parts of rehmannia glutinosa, 6 parts of Chinese date, 1 part of freeze-dried powder of lactobacillus rhamnosus, 1 part of freeze-dried powder of lactobacillus casei;

[0093] The preparation method of the traditional Chinese medicine is the same as that of Example 3.

[0094] Comparative Example 3

[0095] A traditional Chinese medicine, different from Example 3 in that 70 parts of water are used to replace moschus.

[0096] The preparation method of the traditional Chinese medicine is the same as that of Example 3.

[0097] Comparative Example 4

[0098] A traditional Chinese medicine, different from Example 3 in that 70 parts of water are used to replace safflower.

[0099] The preparation method of the traditional Chinese medicine is the same as that of Example 3.

[0100] Comparative Example 5

[0101] A traditional Chinese medicine, calculated by weight parts, comprises the following raw materials: 70 parts of moschus, 70 parts of carthamus tinctorius, 50 parts of notoginseng, 50 parts of cassia bark, 50 parts of lysimachia christinae hance, 50 parts of achyranthes bidentata, 45 parts of eucommia ulmoides, 45 parts of notopterygium incisum, 45 parts of gardenia jasminoides, 45 parts of dragon's bone, 33 parts of poria cocos, 33 parts of codonopsis pilosula, 33 parts of roasted licorice root, 25 parts of white peony root, 25 parts of dried tangerine peel, 25 parts of angelica sinensis, 12 parts of polygonum cuspidatum, 12 parts of polygonatum odoratum, 12 parts of lilium brownii, 12 parts of lygodium japonicum, 6 parts of peach kernel, 6 parts of rehmannia glutinosa, 6 parts of Chinese date, 2 parts of freeze-dried powder of lactobacillus casei;

[0102] The preparation method of the traditional Chinese medicine is the same as that of Example 3.

[0103] Comparative Example 6

[0104] A traditional Chinese medicine, calculated by weight parts, comprises the following raw materials: 70 parts of moschus, 70 parts of carthamus tinctorius, 50 parts of notoginseng, 50 parts of cassia bark, 50 parts of lysimachia christinae hance, 50 parts of achyranthes bidentata, 45 parts of eucommia ulmoides, 45 parts of notopterygium incisum, 45 parts of gardenia jasminoides, 45 parts of dragon's bone, 33 parts of poria cocos, 33 parts of codonopsis pilosula, 33 parts of roasted licorice root, 25 parts of white peony root, 25 parts of dried tangerine peel, 25 parts of angelica sinensis, 12 parts of polygonum cuspidatum, 12 parts of polygonatum odoratum, 12 parts of lilium brownii, 12 parts of lygodium japonicum, 6 parts of peach kernel, 6 parts of rehmannia glutinosa, 6 parts of Chinese date, 2 parts of freeze-dried powder of lactobacillus rhamnosus;

[0105] The preparation method of the traditional Chinese medicine is the same as that of Example 3.

[0106] Comparative Example 7

[0107] A traditional Chinese medicine, calculated by weight parts, comprises the following raw materials: 70 parts of moschus, 70 parts of carthamus tinctorius, 50 parts of notoginseng, 50 parts of cassia bark, 50 parts of lysimachia christinae hance, 50 parts of achyranthes bidentata, 45 parts of eucommia ulmoides, 45 parts of notopterygium incisum, 45 parts of gardenia jasminoides, 45 parts of dragon's bone, 33 parts of poria cocos, 33 parts of codonopsis pilosula, 33 parts of roasted licorice root, 25 parts of white peony root, 25 parts of dried tangerine peel, 25 parts of angelica sinensis, 12 parts of polygonum cuspidatum, 12 parts of polygonatum odoratum, 12 parts of lilium brownii, 12 parts of lygodium japonicum, 6 parts of peach kernel, 6 parts of rehmannia glutinosa, 6 parts of Chinese date;

[0108] The preparation method of the above traditional Chinese medicine comprises the following steps:

[0109] (1) Weigh the raw materials according to the above weight parts and set aside;

[0110] (2) Crush moschus, carthamus tinctorius, notoginseng, cassia bark, lysimachia christinae hance, achyranthes bidentata, eucommia ulmoides, notopterygium incisum, gardenia jasminoides, dragon's bone, poria cocos, codonopsis pilosula, roasted licorice root, white peony root, dried tangerine peel, and angelica sinensis, pass through a 100-mesh sieve, heat and reflux extract with deionized water for 3 times, filter, combine the extractive solutions, and concentrate to obtain Extract I;

[0111] (3) Crush polygonum cuspidatum, polygonatum odoratum, lilium brownii, lygodium japonicum, peach kernel, rehmannia glutinosa, and Chinese date, pass through a 100-mesh sieve, add them to an ethanol aqueous solution, carry out microwave-assisted heat reflux extraction for 3 times, filter, combine the extractive solutions, and concentrate to obtain Extract II;

[0112] (4) Mix Extract I and Extract II, perform ultrasonic treatment, then concentrate into a thick paste, dry in vacuum, pulverize, granulate, and fill into capsules to obtain the product;

[0113] In step (2), the heating temperature is 95 °C, the liquid-solid ratio is 10:1, and the extraction time for each time is 60 min;

[0114] In step (3), the volume concentration of the ethanol aqueous solution is 80%, the heating temperature is 70 °C, the extraction time for each time is 40 min, and the microwave power is 500 W;

[0115] In step (4), the ultrasonic power is 600 W, the frequency is 1.5 MHz, and the time is 30 min;

[0116] In step (4), the relative density of the thick paste is 1.2.

[0117] Experiment 1 Effects of the above traditional Chinese medicines on pain in mice

[0118] 1.1 Effects of traditional Chinese medicines on acetic acid-induced writhing in mice

[0119] Select female Kunming mice, clean grade, with a body weight of 18 - 22 g. Randomly divide them into multiple groups, with 15 mice in each group, including the groups of Examples 1 - 3, the groups of Comparative Examples 1 - 4, and the blank control group. Each group is respectively given intragastric administration at a dose of 0.02 g / 10 g body weight, and the blank control group is given the same weight of distilled water. After 30 min, each mouse is intraperitoneally injected with 0.2 ml / animal of 0.6% acetic acid solution. Observe the number of writhing (including stretching the hind limbs, abdominal contraction and concave upward, and hip elevation) within 15 min after injection. Calculate the average value, and the results are shown in Table 1.

[0120] Table 1 Effects of traditional Chinese medicines on writhing in mice

[0121] Group Number of animals Number of writhing times Blank control group 15 17.8 Example 1 15 4.4 Example 2 15 3.6 Example 3 15 2.8 Comparative example 1 15 6.4 Comparative example 2 15 7.2 Comparative example 3 15 9.5 Comparative example 4 15 8.3

[0122] As can be seen from Table 1, after administration of the traditional Chinese medicines, the number of writhing in mice is significantly less than that in the blank control group, with a significant difference. And the effect of the comparative examples is poor after removing the key medicinal materials, indicating that the main raw materials musk and safflower have a synergistic effect, which is very crucial for the pain-relieving and analgesic effect of the present invention, and their dosages cannot be easily changed. Therefore, the present invention has an obvious analgesic effect on the pain caused by acetic acid.

[0123] 1.2 Effects of traditional Chinese medicines on pain caused by heat stimulation

[0124] Using the hot plate method, set the temperature of the hot plate instrument at 55 °C. After the temperature stabilizes, put 18 - 22 g female mice on the hot plate, and use a stopwatch to record the reaction time (latency period) from when the mice are put on the hot plate until they lick their hind paws as an index of pain threshold, that is, the pain threshold value of the mouse. Randomly divide the mice into multiple groups, with 10 mice in each group. Administer traditional Chinese medicine by gavage, with a dose of 0.02 g / 10 g body weight. The blank control group is given the same weight of distilled water. Measure the pain threshold of each mouse 2 times before administration, and take the average value as the pain threshold value of the mouse. Measure the pain threshold once at the 30th minute and 60th minute after administration respectively. Compare the differences in pain threshold values at each time point with those before administration.

[0125] Table 2 Effects of Traditional Chinese Medicine on Pain Induced by Thermal Stimulation

[0126]

[0127] As can be seen from Table 2, the pain threshold 60 minutes after administration of the traditional Chinese medicine of the present invention is significantly higher than that before administration, and there are also significant differences compared with the pain threshold change value of the blank control group. While the analgesic effect of the comparative example after removing the main raw materials is poor, that is, the present invention cannot fully exert its medicinal effect without the key components, and the raw materials in the formula cannot be replaced. Therefore, the traditional Chinese medicine of the present invention has an obvious analgesic effect on pain caused by thermal stimulation.

[0128] Experiment 2 Effects of Traditional Chinese Medicine on the Immune Function of Mice

[0129] Select female Kunming mice of clean grade with a body weight of 18 - 22 g. Randomly divide them into multiple groups, with 10 mice in each group. The mice in the blank control group are given normal saline by gavage, and the rest are all intraperitoneally injected with cyclophosphamide at 80 mg / (kg·d) for 3 days for modeling.

[0130] The blank control group and the model control group are given 0.9% normal saline by gavage once a day for 17 days after modeling; the rest are given traditional Chinese medicine by gavage after modeling, with a dose of 0.02 g / 10 g body weight, once a day for 17 days.

[0131] 2.1 Detection of Body Fat Content and Immune Organ Index

[0132] Weigh the initial body weight and final body weight of the mice with a balance. After taking blood from the eye socket, decapitate the mice, dissect the spleen and thymus and weigh them with a balance, calculate the spleen index and thymus index. Fast the mice for 12 hours without water after the last administration, and weigh all the mice. Take blood from the eye socket, separate the serum, and store it at -20 °C for later use; decapitate the mice by cervical dislocation, take their thymus and spleen, wash and dry them, and then weigh them, and calculate the immune organ index: thymus (spleen) index = thymus (spleen) weight (mg) / body weight (g). The results are shown in Table 3.

[0133] Table 3 Effects of Traditional Chinese Medicine on the Immune Organ Index of Immunosuppressed Mice

[0134] Group Number of animals Spleen index (mg / g) Thymus index (mg / g) Blank control group 10 2.60 2.07 Model control group 10 1.83 1.66 Example 1 10 2.48 1.91 Example 2 10 2.51 1.95 Example 3 10 2.55 2.03 Comparative example 5 10 2.04 1.82 Comparative example 6 10 1.98 1.73 Comparative example 7 10 1.87 1.66

[0135] As shown in Table 3, the spleen index and thymus index of the model control group mice were reduced and significantly lower than those of the blank control group, indicating that the model control group mice were successfully modeled and were in an immunosuppressive state. The immune organ indexes of the Chinese medicine mice of the present invention were all increased to varying degrees, and the correction degree of Example 3 was the best. After the comparison ratio changed the probiotic dosage or ratio, the correction effect was weakened. Since the weight and coefficient of the immune organs are positively correlated with the immune level of the body, the above results show that the use of probiotic combination and Chinese medicine combination can improve the immune level of mice. Therefore, the probiotic combination of the present invention and Chinese medicinal materials produce a synergistic effect and can jointly improve immunity.

[0136] 2.2 Detection of spleen NK cell ratio

[0137] 5 mL of fasting venous blood was extracted from the mice and sent for testing after anticoagulation. During the test, 150 μL of anticoagulated blood was extracted from the test tube, 30 μL of monoclonal antibodies with different fluorescent labels were added in the dark, and the mixture was fully mixed and stained in the dark at room temperature for 20 minutes. 3 mL of hemolysin was added, and the mixture was centrifuged at 1000 r / min for 10 minutes, and the supernatant was discarded. 500 μL of detergent was added to resuspend the cells and tested by flow cytometry. The results are shown in Table 4.

[0138] 2.3NK cell activity detection

[0139] MTT colorimetric method was used to detect the activity of mouse spleen NK cells: fresh spleen tissue was obtained under sterile operation, blood was rinsed with pre-cooled 0.9% NaCl solution, filter paper was used to dry, 60 mg of tissue was taken, 0.6 mL of pre-cooled PBS was added, and the mixture was fully ground in an ice box for 30 min, centrifuged at 4°C and 2500 r / min for 10 min, and the supernatant was taken and stored at -70°C. The spleen cell suspension was diluted with 1640 culture medium containing 10% bovine serum to 1×10 6 / mL, target cells were incubated with K562 (1-2 days in advance), and the cell number was adjusted to 1×10 5 / mL. Use a pipette to draw 100 μL of effector and target cells in a 96-well culture plate and incubate for 30 min. Add 100 μL of effector cells and 1640 culture medium to each well of the effector cell control well, and add 100 μL of target cells and 1640 culture medium to each well of the target cell control well. Each specimen has 5 replicate wells. Add 10 μL MTT to each well and incubate at 37°C, 5% CO 2Co-incubate for 4 h under incubator conditions; after incubation, aspirate and discard the supernatant, add 150 μL of DMSO and 20 μL of glycine buffer (pH 10.5) to each well, and shake on a shaker at medium speed for 20 min. Calculate the absorbance value of each well using an ELISA reader at a wavelength of 570 nm. The NK cell activity was calculated as the mean of 5 replicates for each specimen. The results are shown in Table 4.

[0140] Table 4 Effects of Traditional Chinese Medicines on Spleen NK Cells in Immunosuppressed Mice

[0141] Group Number of animals Proportion of NK cells Activity of NK cells Blank control group 10 3.67 55.73 Model control group 10 1.89 30.15 Example 1 10 3.04 49.82 Example 2 10 3.27 52.19 Example 3 10 3.58 53.96 Comparative example 5 10 2.65 45.64 Comparative example 6 10 2.46 42.51 Comparative example 7 10 2.03 39.72

[0142] As can be seen from Table 4, compared with the blank control group, the proportion and activity of NK cells in the spleen of mice in the model group were significantly decreased, indicating that the establishment of the immunosuppressed mouse model was successful. After treatment with the traditional Chinese medicines in the examples, the proportion and activity of NK cells in mice were significantly increased, proving that the present invention can repair damaged immune organs, improve the immunosuppressed state of the body, and enhance the immune function of the body. Although the comparative example had a certain improvement effect, the effect was significantly weaker than that of the examples, indicating that the immune-enhancing drug effect is the result of the synergistic compatibility of multiple components.

[0143] The various embodiments in this specification are described in a progressive manner. Each embodiment focuses on the differences from other embodiments. The same or similar parts among the various embodiments can be referred to each other.

[0144] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A Chinese medicine for enhancing immunity and treating pain symptoms, characterized in that: The following raw materials are included: musk, safflower, Panax notoginseng, cinnamon, desmodium, Achyranthes bidentata, Eucommia ulmoides, Notopterygium wilfordii, Gardenia jasminoides, dragon bone, Poria cocos, Codonopsis pilosula, licorice, white peony root, tangerine peel, angelica, Polygonum cuspidatum, Polygonatum odoratum, lily, Lygodium japonicum, peach kernel, Rehmannia glutinosa, jujube, Lactobacillus rhamnosus freeze-dried powder, and Lactobacillus casei freeze-dried powder.

2. The Chinese medicine according to claim 1, characterized in that The invention comprises the following raw materials in parts by weight: 60-80 parts of musk, 60-80 parts of safflower, 45-60 parts of panax notoginseng, 45-55 parts of cinnamon bark, 40-55 parts of chrysanthemum, 40-55 parts of achyranthes bidentata, 40-50 parts of eucommia bark, 40-50 parts of notopterygium root, 40-50 parts of gardenia, 40-50 parts of dragon bone, 30-35 parts of tuckahoe, 30-35 parts of codonopsis pilosula, 30-35 parts of roasted liquorice, 20-30 parts of white peony root, 20-30 parts of tangerine peel, 20-30 parts of angelica sinensis, 10-15 parts of knotweed, 10-15 parts of polygonatum odoratum, 10-15 parts of lily, 10-15 parts of sea sand, 5-8 parts of peach kernel, 5-8 parts of rehmannia root, 5-8 parts of jujube, 0.5-5 parts of Lactobacillus rhamnosus freeze-dried powder, and 0.5-5 parts of Lactobacillus casei freeze-dried powder.

3. The Chinese medicine according to claim 2, characterized in that: The invention comprises the following raw materials in parts by weight: 70 parts of musk, 70 parts of safflower, 50 parts of Panax notoginseng, 50 parts of cinnamon bark, 50 parts of Lycopodiella cuneata, 50 parts of Achyranthes bidentata, 45 parts of Eucommia bark, 45 parts of Notopterygium wilfordii, 45 parts of Gardenia jasminoides, 45 parts of Dragon bone, 33 parts of Poria cocos, 33 parts of Codonopsis pilosula, 33 parts of Radix Glycyrrhizae Preparata, 25 parts of White Peony Root, 25 parts of Tangerine Peel, 25 parts of Angelica sinensis, 12 parts of Polygonatum cuspidatum, 12 parts of Polygonatum odoratum, 12 parts of Lilium bulgur, 12 parts of Lygodium japonicum, 6 parts of Peach Kernel, 6 parts of Rehmannia glutinosa, 6 parts of Jujube, 1 part of Lactobacillus rhamnosus freeze-dried powder, and 1 part of Lactobacillus casei freeze-dried powder.

4. The Chinese medicine according to claim 1, characterized in that The viable count of Lactobacillus rhamnosus in the Lactobacillus rhamnosus freeze-dried powder is 10 8 ~10 9 CFU / g, the number of viable Lactobacillus casei in the freeze-dried Lactobacillus casei powder is 10 8 ~10 9 CFU / g.

5. The method for preparing the Chinese medicine according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Weigh the raw materials according to the above weight parts and set aside; (2) Grind musk, safflower, Panax notoginseng, cinnamon bark, Lysimachia chinensis, Achyranthes bidentata, Eucommia bark, Notopterygium wilfordii, Gardenia jasminoides, Dragon bone, Poria cocos, Codonopsis pilosula, Radix glycyrrhizae, white peony root, dried orange peel, and Angelica sinensis, pass through an 80-100 mesh sieve, extract with deionized water by heating and refluxing for 2-4 times, filter, combine the extracts, and concentrate to obtain extract I; (3) Grind Polygonum cuspidatum, Polygonatum odoratum, Lilium sibiricum, Lygodium japonicum, Prunus persica, Rehmannia glutinosa, and jujube, pass through an 80-100 mesh sieve, add to an ethanol aqueous solution, perform microwave-assisted heating reflux extraction 2-4 times, filter, combine the extracts, and concentrate to obtain extract II; (4) The extract I and the extract II are mixed, ultrasonically treated, and then concentrated into a thick paste. After cooling, freeze-dried powder of Lactobacillus rhamnosus and freeze-dried powder of Lactobacillus casei are added, the mixture is mixed and stirred, and then vacuum dried, crushed, granulated, and filled into capsules to obtain the product.

6. The preparation method according to claim 5, characterized in that: The heating temperature in step (2) is 91-97° C., the liquid-to-solid ratio is 8-12:1, and the extraction time is 30-90 minutes each time.

7. The preparation method according to claim 5, characterized in that: The volume concentration of the ethanol aqueous solution in step (3) is 70% to 90%, the heating temperature is 65 to 75° C., the extraction time is 30 to 60 minutes each time, and the microwave power is 300 to 800W.

8. The preparation method according to claim 5, characterized in that: In step (4), the power of the ultrasound is 500-800 W, the frequency is 1.5-2 MHz, and the time is 30-40 min; The relative density of the thick paste is 1.2 to 1.

25.

9. The preparation method according to claim 4, characterized in that: The stirring speed in step (4) is 300 to 500 r / min and the stirring time is 60 to 120 min.

10. The use of the traditional Chinese medicine according to any one of claims 1 to 3, characterized in that: The use is to prepare medicines for enhancing immunity and treating pain.

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