Traditional Chinese medicine composition for inhibiting staphylococcus aureus as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition for inhibiting staphylococcus aureus as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from, by mass, 1-10 parts of clove, 1-10 parts of herba pyrolae, 1-5 parts of caulis sinomenii, 1-5 parts of caulis polygoni multiflori, 1-3 parts of radix actinidiae chinensis, 1-3 parts of rhizoma smilacis glabrae and 1-3 parts of folium artemisiae argyi. The traditional Chinese medicine composition disclosed by the invention has a remarkable inhibiting effect on staphylococcus aureus regardless of a standard strain or a clinical multi-drug-resistant strain, and drug resistance is not easy to generate; the traditional Chinese medicine composition disclosed by the invention also has a synergistic effect with erythrocin, and can be combined for external use to inhibit staphylococcus aureus.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for inhibiting Staphylococcus aureus, a preparation method thereof, and an application thereof. Background Art

[0002] Bacterial infection is a disease caused by the invasion, growth and reproduction of pathogenic bacteria or conditional pathogenic bacteria and the production of toxins. Antibiotics are the main drugs for treating bacterial infections, but the overuse of antibiotics will exert selective pressure on bacteria, leading to the generation of bacterial drug resistance. In recent years, the application of traditional Chinese medicine extracts in inhibiting Staphylococcus aureus has received more attention. The treatment of bacterial infections with traditional Chinese medicine extracts has the characteristics of rich active ingredients and difficult to produce drug resistance, which is of great significance for improving the problem of bacterial drug resistance.

[0003] Staphylococcus aureus is an important pathogenic bacterium that causes human infections and bacterial food poisoning, and can directly cause suppurative infections, pneumonia, pericarditis, septicemia, pyosepticemia, etc. Staphylococcus aureus is relatively easy to develop drug resistance, which causes great difficulties for effective clinical treatment. At present, the effect of traditional Chinese medicine in treating Staphylococcus aureus infections still needs to be improved. Therefore, the development of a traditional Chinese medicine composition with effective inhibitory effect on Staphylococcus aureus is of great significance for the treatment of Staphylococcus aureus infections. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a traditional Chinese medicine composition for inhibiting Staphylococcus aureus, a preparation method thereof, and an application thereof, which has a significant inhibitory effect on Staphylococcus aureus and is not easy to produce drug resistance.

[0005] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0006] A traditional Chinese medicine composition for inhibiting Staphylococcus aureus, comprising the following raw materials by mass parts: 1-10 parts of clove, 1-10 parts of pyrola herb, 1-5 parts of sinomenium acutum, 1-5 parts of polygonum multiflorum thunb. stem, 1-3 parts of actinidia arguta root, 1-3 parts of smilax glabra and 1-3 parts of mugwort leaf.

[0007] Preferably, the raw materials are water extracts of crude drugs.

[0008] The present invention also provides a preparation method of the above-mentioned traditional Chinese medicine composition, comprising the following steps: the crude drugs of the raw materials are respectively decocted with water to obtain decoction liquid, concentrated and dried, and then the water extracts of the crude drugs are mixed by mass parts.

[0009] Preferably, the water decoction is carried out 2-3 times, the ratio of material to liquid is 1g:5-40mL, and each decoction is carried out for 20-50min.

[0010] More preferably, the water decoction is carried out 3 times; the material-liquid ratio for the first water decoction is 1 g: 5-40 mL, and the decoction time is 25-35 min. The material-liquid ratios for the second and third water decoctions are 1 g: 10-25 mL, and the decoction time for each time is 35-45 min.

[0011] The present invention also provides an application of the traditional Chinese medicine composition in preparing a drug for inhibiting Staphylococcus aureus.

[0012] The present invention also provides a drug for inhibiting Staphylococcus aureus, which contains the traditional Chinese medicine composition.

[0013] Preferably, the content of the traditional Chinese medicine composition is ≥ 3 mg / mL.

[0014] Preferably, the drug is an external preparation, including ointment, cream, solution, spray, gel, powder, lotion and patch.

[0015] More preferably, the drug further contains pharmaceutically acceptable excipients.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The components of the traditional Chinese medicine composition of the present invention have a synergistic effect, and show a significant inhibitory effect on Staphylococcus aureus, whether it is a standard strain or a clinical multi-drug resistant strain. The traditional Chinese medicine composition of the present invention also has a synergistic effect with erythromycin and can be jointly used to inhibit Staphylococcus aureus in vitro.

[0018] The overuse of antibiotics has led to an increasingly serious problem of bacterial drug resistance. The traditional Chinese medicine composition of the present invention has unique advantages in inhibiting Staphylococcus aureus. Since its components are derived from traditional Chinese medicine, compared with traditional antibiotics, it is not easy to cause drug resistance in Staphylococcus aureus, and can effectively solve the problem of bacterial drug resistance, providing a new and reliable option for clinical treatment of Staphylococcus aureus infections. Description of the Drawings

[0019] Figure 1 It is the antibacterial results of different drug treatment groups for inhibiting Staphylococcus aureus in Test Example 1. Among them, A, D: clinical multi-drug resistant strain (2020052111), B, C: standard strain (ATCC 25923), 1: Example 1, 2: Example 2, 3: Example 3, 4: Comparative Example 1, 5: Comparative Example 2, 6: Comparative Example 3, 7: saline control, 8: Example 4, 9: Example 5, 10: Comparative Example 4, 11: Comparative Example 5;

[0020] Figure 2The antibacterial results of different medicated groups in Test Example 2 for inhibiting Staphylococcus aureus are as follows. Among them, E15: 15 μg erythromycin paper disc, P10: 10 unit penicillin paper disc, red circle: the traditional Chinese medicine composition in Comparative Examples 6-7. Detailed implementation manners

[0021] The present invention provides a traditional Chinese medicine composition for inhibiting Staphylococcus aureus, which comprises the following raw medicinal materials by mass parts: 1-10 parts of clove, 1-10 parts of pyrola, 1-5 parts of sinomenium acutum, 1-5 parts of polygonum multiflorum thunb. stem, 1-3 parts of actinidia chinensis planch. root, 1-3 parts of smilax glabra and 1-3 parts of artemisia argyi; preferably, the raw medicinal materials are water extracts of each crude drug.

[0022] Clove is the flower bud of Syzygium aromaticum of Myrtaceae, and its main antibacterial components are eugenol and flavonoids. It is pungent and warm. It enters the lung, stomach, spleen and kidney meridians. It can warm the middle-jiao to arrest reverse flow, warm the kidney to assist yang, and disperse cold and relieve pain. In the present invention, preferably, the traditional Chinese medicine composition contains 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts of clove or water extract of clove, and more preferably 8 parts.

[0023] Pyrola is the dried whole herb of Pyrola rotundifolia L. or Pyrola decorata H. Andr. of Pyrolaceae, and its main antibacterial components are flavonoids and arbutin. It is bitter and flat. It belongs to the liver and kidney meridians. It can dispel wind-dampness, tonify the liver and kidney, strengthen the muscles and bones, and stop bleeding. In the present invention, preferably, the traditional Chinese medicine composition contains 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts of pyrola or water extract of pyrola, and more preferably 5 parts.

[0024] Sinomenium acutum is the vine stem of Sinomenium acutum Rehd. et Wils. or Sinomenium acutum var. cinereum Rehd. et Wils. of Menispermaceae, and its main antibacterial components are sinomenine and alkaloids. It is bitter, pungent and flat. It belongs to the liver and spleen meridians. It can dispel wind-dampness, dredge the meridians and promote urination. In the present invention, preferably, the traditional Chinese medicine composition contains 1 part, 2 parts, 3 parts, 4 parts or 5 parts of sinomenium acutum or water extract of sinomenium acutum, and more preferably 5 parts.

[0025] Polygonum multiflorum thunb. stem is the dried vine stem of Polygonum multiflorum Thunb. of Polygonaceae, and its main antibacterial component is anthraquinone. It is sweet and flat. It belongs to the heart and liver meridians. It can nourish blood and soothe the nerves, dispel wind and dredge the collaterals. In the present invention, preferably, the traditional Chinese medicine composition contains 1 part, 2 parts, 3 parts, 4 parts or 5 parts of polygonum multiflorum thunb. stem or water extract of polygonum multiflorum thunb. stem, and more preferably 2 parts.

[0026] Actinidia chinensis planch. root is the root of Actinidia chinensis Planch. of Actinidiaceae, and its main antibacterial component is triterpenoid saponin. It is slightly sour, sweet and cool. It is slightly poisonous. It belongs to the liver, gallbladder, spleen and stomach meridians. It can clear heat and detoxify, dispel wind and dampness, and promote diuresis and stop bleeding. In the present invention, preferably, the traditional Chinese medicine composition contains 1 part, 2 parts or 3 parts of actinidia chinensis planch. root or water extract of actinidia chinensis planch. root, and more preferably 1 part.

[0027] Smilax glabra Roxb., the tuber of Smilax glabra Roxb. of the genus Smilax in the Liliaceae family, has saponins as its main antibacterial components. It is sweet, light in taste, and neutral in nature. It acts on the liver and stomach meridians. It can detoxify, eliminate dampness, and promote the flow of joints. In the present invention, it is preferred that the traditional Chinese medicine composition contains 1 part, 2 parts or 3 parts of Smilax glabra Roxb. or its water extract, and more preferably 1 part.

[0028] Artemisia argyi Levl. et Vant., the leaf of Artemisia argyi Levl. et Vant. of the Compositae family, has volatile oils and flavonoids as its main antibacterial components. It is pungent, bitter, and warm in nature. It is slightly poisonous. It acts on the liver, spleen, and kidney meridians. It can warm the meridians to stop bleeding, disperse cold and regulate menstruation, and prevent miscarriage. In the present invention, it is preferred that the traditional Chinese medicine composition contains 1 part, 2 parts or 3 parts of Artemisia argyi Levl. et Vant. or its water extract, and more preferably 1 part.

[0029] The components of the traditional Chinese medicine composition of the present invention play a synergistic antibacterial role, and synergistically inhibit Staphylococcus aureus through multiple pathways such as destroying cell membranes, inhibiting the activity of key enzymes, interfering with nucleic acid / protein synthesis, inhibiting virulence factors and biofilm formation. The traditional Chinese medicine composition of the present invention is preferably used externally in combination with erythromycin to synergistically inhibit Staphylococcus aureus.

[0030] The present invention provides a preparation method of the above-mentioned traditional Chinese medicine composition, including the following steps: decocting the crude drugs of each raw material medicine with water to obtain a decoction, concentrating and drying, and then mixing the water extracts of the raw material medicines according to parts by mass.

[0031] In the present invention, it is preferred that before decocting, the crude drugs of each raw material medicine are respectively soaked in clear water for 0.5 - 2 h, and more preferably for 1 h. As an implementable method, before decocting, the crude drug slices of each raw material medicine are placed in cold water for soaking, and the drugs floating on the water surface are turned up and down with bamboo chopsticks to completely immerse the traditional Chinese medicine slices in water, ensuring that the water surface is 2 - 3 cm higher than the raw material medicine slices.

[0032] In the present invention, it is preferred that the water decoction is carried out 2 - 3 times, the ratio of material to liquid is 1 g:5 - 40 mL, and each decoction is carried out for 20 - 50 min. More preferably, the water decoction is carried out 3 times; the ratio of material to liquid for the first decoction is 1 g:5 - 40 mL, and the decoction is carried out for 25 - 35 min, and the ratio of material to liquid for the second and third decoctions is 1 g:10 - 25 mL, and each decoction is carried out for 35 - 45 min. Even more preferably, the water decoction is carried out 3 times; the ratio of material to liquid for the first decoction is 1 g:30 mL, and the decoction is carried out for 30 min, and the ratio of material to liquid for the second and third decoctions is 1 g:20 mL, and each decoction is carried out for 40 min. As an implementable method, during decocting, first bring to a boil with high heat and then decoct with low heat.

[0033] In the present invention, it is preferred to filter and mix the decoction after decocting, and more preferably filter with a 100 - mesh sieve.

[0034] In the present invention, it is preferred to use boiling concentration for concentration.

[0035] In the present invention, freeze-drying is preferably used for drying, and more preferably, the temperature of freeze-drying is -40°C and the time is 8 h.

[0036] In the present invention, it is preferred to perform grinding after drying, and more preferably, grind to 50 mesh. As an implementable method, the solid after freeze-drying is ground into powder by a grinder, put into a sealed bag, and stored in a refrigerator at 4°C for later use.

[0037] The present invention also provides an application of the traditional Chinese medicine composition in the preparation of a drug for inhibiting Staphylococcus aureus.

[0038] The present invention also provides a drug for inhibiting Staphylococcus aureus, which contains the traditional Chinese medicine composition, and preferably the content of the traditional Chinese medicine composition is ≥ 3 mg / mL.

[0039] In the present invention, the drug is preferably an external preparation, including ointment, cream, solution, spray, gel, powder, lotion and patch. More preferably, the drug also contains pharmaceutically acceptable excipients.

[0040] The technical solutions provided by the present invention are described in detail below with reference to the examples, but they should not be construed as limiting the protection scope of the present invention.

[0041] Example 1

[0042] A traditional Chinese medicine composition for inhibiting Staphylococcus aureus is prepared as follows:

[0043] (1) Prepare the crude drugs of cloves, Pyrola calliantha, Sinomenium acutum, Polygonum multiflorum Thunb. var. multiflorum, Actinidia chinensis Planch., Smilax glabra and Artemisia argyi.

[0044] (2) Immerse the crude drugs in step (1) in cold water for 1 h, and turn the drugs floating on the water surface up and down with bamboo chopsticks to completely immerse the Chinese medicine pieces in water, ensuring that the water surface is 2-3 cm higher than the Chinese medicine.

[0045] (3) Put the soaked crude drugs into stainless steel containers respectively, add water at a ratio of 1 g:30 mL for the first decoction, first bring to a boil over high heat, and then simmer for 30 min; after filtering the first decoction liquid, add water at a ratio of 1 g:20 mL to the filter residue for the second decoction, first bring to a boil over high heat, and then simmer for 40 min; after filtering the second decoction liquid, add water at a ratio of 1 g:20 mL to the filter residue for the third decoction, first bring to a boil over high heat, and then simmer for 40 min.

[0046] (4) Filter the three decoction liquids in step (3) through a 100-mesh sieve and mix them, boil and concentrate, and obtain a solid by freeze-drying (-40°C, 8 h), and grind the solid into powder with a particle size of 50 mesh by a grinder.

[0047] (5) Mix 8 parts of the water extract of Eugenia caryophyllata Thunb., 5 parts of the water extract of Pyrola calliantha H. Andr., 5 parts of the water extract of Sinomenium acutum (Thunb.) Rehd. et Wils., 2 parts of the water extract of Polygonum multiflorum Thunb. var. thomsonii (Wall.) Stew., 1 part of the water extract of Actinidia chinensis Planch., 1 part of the water extract of Smilax glabra Roxb. and 1 part of the water extract of Artemisia argyi H. Lév. & Vaniot to obtain a traditional Chinese medicine composition for inhibiting Staphylococcus aureus.

[0048] Example 2

[0049] A traditional Chinese medicine composition for inhibiting Staphylococcus aureus, the preparation method is the same as that of Example 1, the difference is: 5 parts of the water extract of Eugenia caryophyllata Thunb., 5 parts of the water extract of Pyrola calliantha H. Andr., 1 part of the water extract of Sinomenium acutum (Thunb.) Rehd. et Wils., 2 parts of the water extract of Polygonum multiflorum Thunb. var. thomsonii (Wall.) Stew., 1 part of the water extract of Actinidia chinensis Planch., 1 part of the water extract of Smilax glabra Roxb. and 1 part of the water extract of Artemisia argyi H. Lév. & Vaniot.

[0050] Example 3

[0051] A traditional Chinese medicine composition for inhibiting Staphylococcus aureus, the preparation method is the same as that of Example 1, the difference is: 5 parts of the water extract of Eugenia caryophyllata Thunb., 10 parts of the water extract of Pyrola calliantha H. Andr., 3 parts of the water extract of Sinomenium acutum (Thunb.) Rehd. et Wils., 3 parts of the water extract of Polygonum multiflorum Thunb. var. thomsonii (Wall.) Stew., 3 parts of the water extract of Actinidia chinensis Planch., 1 part of the water extract of Smilax glabra Roxb. and 2 parts of the water extract of Artemisia argyi H. Lév. & Vaniot.

[0052] Example 4

[0053] A traditional Chinese medicine composition for inhibiting Staphylococcus aureus, the preparation method is the same as that of Example 1, the difference is: 5 parts of the water extract of Eugenia caryophyllata Thunb., 10 parts of the water extract of Pyrola calliantha H. Andr., 3 parts of the water extract of Sinomenium acutum (Thunb.) Rehd. et Wils., 2 parts of the water extract of Polygonum multiflorum Thunb. var. thomsonii (Wall.) Stew., 3 parts of the water extract of Actinidia chinensis Planch., 1 part of the water extract of Smilax glabra Roxb. and 1 part of the water extract of Artemisia argyi H. Lév. & Vaniot. Additionally, prepare 1 piece of cefoxitin disc (FOX, 30 μg / piece).

[0054] Example 5

[0055] A traditional Chinese medicine composition for inhibiting Staphylococcus aureus, the preparation method is the same as that of Example 1, the difference is: 2 parts of the water extract of Eugenia caryophyllata Thunb., 10 parts of the water extract of Pyrola calliantha H. Andr., 5 parts of the water extract of Sinomenium acutum (Thunb.) Rehd. et Wils., 3 parts of the water extract of Polygonum multiflorum Thunb. var. thomsonii (Wall.) Stew., 3 parts of the water extract of Actinidia chinensis Planch., 1 part of the water extract of Smilax glabra Roxb. and 2 parts of the water extract of Artemisia argyi H. Lév. & Vaniot. Additionally, prepare 1 piece of cefoxitin disc (FOX, 30 μg / piece).

[0056] Comparative Example 1

[0057] Only the water extract of Pyrola calliantha H. Andr.

[0058] Comparative Example 2

[0059] Only the water extract of Polygonum multiflorum Thunb. var. thomsonii (Wall.) Stew.

[0060] Comparative Example 3

[0061] Only the water extract of Eugenia caryophyllata Thunb.

[0062] Comparative Example 4

[0063] Same as Example 1, with the differences being: 2 parts of water extract of Pyrola calliantha Hance, 5 parts of water extract of Sinomenium acutum Rehd. et Wils., 1 part of water extract of Polygonum multiflorum Thunb. var. caudatum W. T. Wang et S. H. Wang, 1 part of water extract of Smilax glabra Roxb., and 2 parts of water extract of Artemisia argyi Levl. et Vant. Additionally, prepare 1 cefoxitin disc (FOX, 30 μg / disc).

[0064] Control Example 5

[0065] Only 1 cefoxitin disc (FOX, 30 μg / disc).

[0066] Control Example 6

[0067] A traditional Chinese medicine composition for inhibiting Staphylococcus aureus, with the preparation method being the same as that in Example 1, with the differences being: 3 parts of water extract of Eugenia caryophyllata Thunb., 2 parts of water extract of Pyrola calliantha Hance, 1 part of water extract of Sinomenium acutum Rehd. et Wils., 5 parts of water extract of Polygonum multiflorum Thunb. var. caudatum W. T. Wang et S. H. Wang, 1 part of water extract of Actinidia chinensis Planch. var. hispida C. F. Liang, 1 part of water extract of Smilax glabra Roxb., and 1 part of water extract of Artemisia argyi Levl. et Vant. Additionally, prepare 1 erythromycin disc (15 μg / disc).

[0068] Control Example 7

[0069] A traditional Chinese medicine composition for inhibiting Staphylococcus aureus, with the preparation method being the same as that in Example 1, with the differences being: 3 parts of water extract of Eugenia caryophyllata Thunb., 2 parts of water extract of Pyrola calliantha Hance, 1 part of water extract of Sinomenium acutum Rehd. et Wils., 5 parts of water extract of Polygonum multiflorum Thunb. var. caudatum W. T. Wang et S. H. Wang, 1 part of water extract of Actinidia chinensis Planch. var. hispida C. F. Liang, 1 part of water extract of Smilax glabra Roxb., and 1 part of water extract of Artemisia argyi Levl. et Vant. Additionally, prepare 1 penicillin disc (10 unit / disc).

[0070] Test Example 1

[0071] Referring to "Clinical Microbiology Diagnosis and Illustration, 4th Edition" (edited by Zhou Tingyin and Zhang Qiangqiang, Shanghai Science and Technology Press, September 2017), the inhibitory activity of the traditional Chinese medicine composition against Staphylococcus aureus was detected by the inhibition zone method (diffusion method). The specific method is as follows:

[0072] (1) Activation of the bacterial strain and preparation of the bacterial solution: Inoculate the cryopreserved Staphylococcus aureus bacterial strain by streaking on a nutrient agar plate and culture it overnight at 35°C; pick a single colony and inoculate it into 10 mL of nutrient broth liquid medium, and culture it overnight at 35°C and 200 r / min on a shaker. The bacterial solution is reserved.

[0073] (2) Coating the plate: Dilute the bacterial solution in step (1) with sterile physiological saline to 0.5 McFarland turbidity. Dip a sterile swab into the diluted bacterial solution and rotate and squeeze it on the tube wall to remove the excess bacterial solution, and then evenly spread and inoculate it on the surface of the nutrient agar plate (product of Guangzhou Dijing Company) three times. Each time, rotate the plate 60°, and finally go around the periphery of the petri dish twice to ensure uniform coating of the entire culture medium surface.

[0074] (3) Preparation of the traditional Chinese medicine composition solution: Dissolve the powders of the traditional Chinese medicine compositions in Examples 1 - 5 and Comparative Examples 1 - 5 with deionized water. The concentration of the traditional Chinese medicine composition solution is 200 mg / mL. After autoclaving, it is reserved for use.

[0075] (4) Use a sterilized punch to punch holes in the solid medium coated with the bacterial solution, with a diameter of 6 mm; add the traditional Chinese medicine composition liquid prepared in step (3) into the holes, 80 μL per hole. In Examples 4 and 5 and Comparative Example 5, use sterile forceps to stick cefoxitin paper on the agar surface and gently press to ensure contact.

[0076] (5) Place the petri dish in a constant temperature incubator at 35 °C and continue to culture overnight, then measure the diameter of the inhibition zone against Staphylococcus aureus.

[0077] Test results:

[0078] The measured results of the diameter of the inhibition zone against Staphylococcus aureus in different treatment groups are shown in Table 1, Figure 1 as shown. It can be seen that the standard strain is sensitive to all groups, but Example 1 still maintains a relative advantage, indicating that the antibacterial mechanism of the traditional Chinese medicine composition of the present invention does not depend on a specific bacterial metabolic pathway. The inhibitory effect of Example 1 on clinical multi - drug - resistant strains is 31.3% higher than that of Comparative Example 5, proving that the traditional Chinese medicine composition of the present invention can bypass the drug - resistant pathway of traditional β - lactam antibiotics and exert an independent antibacterial mechanism.

[0079] Table 1 Diameter of the inhibition zone against Staphylococcus aureus in different treatment groups

[0080]

[0081] Test Example 2

[0082] Refer to "Clinical Microbiology Diagnosis and Illustration, 4th Edition" (edited by Zhou Tingyin and Zhang Qiangqiang, Shanghai Science and Technology Press, September 2017), and use the disk diffusion method to conduct the combined drug sensitivity test of the traditional Chinese medicine composition of the present invention with erythromycin and penicillin. The specific method is as follows:

[0083] Steps (1) - (5) are the same as in Test Example 1. Among them, in step (4): In Comparative Example 6 and Comparative Example 7, use sterile forceps to stick erythromycin paper (15 μg / tablet) and penicillin paper (10 unit / tablet) on the agar surface and gently press to ensure contact. Report "synergistic", "additive", "irrelevant" or "antagonistic" effects according to the presented inhibition zone pattern.

[0084] Test results:

[0085] The results of the combined drug sensitivity test of different treatment groups against Staphylococcus aureus are as Figure 2As shown, it can be seen that the traditional Chinese medicine composition of the present invention has an obvious "synergistic" effect with erythromycin and an "additive" effect with penicillin, indicating that the traditional Chinese medicine composition of the present invention can be used externally in combination with erythromycin to inhibit Staphylococcus aureus.

[0086] Test Example 3

[0087] Referring to "Clinical Microbiology Diagnosis and Illustration, 4th Edition" (edited by Zhou Tingyin and Zhang Qiangqiang, Shanghai Science and Technology Press, September 2017), the microbroth dilution method was used to detect the activity of the traditional Chinese medicine composition against Staphylococcus aureus. The specific method is as follows:

[0088] (1) Preparation of the traditional Chinese medicine composition and the culture medium: The powders of the traditional Chinese medicine compositions in Examples 1 - 3 and Comparative Examples 1 - 3 were dissolved in deionized water to 400 mg / mL to obtain the traditional Chinese medicine composition solution; Nutrient Broth (product of Qingdao Haibo Biotechnology Co., Ltd.) was prepared according to the instructions and sterilized by autoclaving for later use.

[0089] (2) The traditional Chinese medicine composition solutions of each example and comparative example were serially diluted with sterile nutrient broth. The concentration of the first well was 400 mg / mL, with a total of 11 dilution degrees; The overnight - cultured Staphylococcus aureus bacterial solution was diluted with nutrient broth to 0.5 McFarland turbidity standard, and 100 μL was added to each well, then 100 μL of the traditional Chinese medicine composition solution was added. The concentrations of the traditional Chinese medicine composition from the first well to the eleventh well were 200, 100, 50, 25, 12.5, 6.25, 3.13, 1.57, 0.79, 0.4, and 0.2 mg / mL respectively. 100 μL of normal saline was added to the positive control well; The culture plate was placed in an incubator at 35°C and cultured overnight, and the results were observed.

[0090] (3) Result determination: The MIC (the lowest drug concentration that can completely inhibit bacterial growth) of the traditional Chinese medicine composition in each group was determined. Among them, the bacterial growth test in the positive control well needed to be significant. If there were multiple skipped wells, the results would not be reported and the test should be repeated.

[0091] Test results:

[0092] The MIC determination results of the traditional Chinese medicine compositions in Examples 1 - 3 and Comparative Examples 1 - 3 are shown in Table 2. It can be seen that there is a synergistic effect among the components of the traditional Chinese medicine composition of the present invention.

[0093] Table 2 MIC determination results of different traditional Chinese medicine compositions

[0094]

[0095] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A Chinese medicine composition for inhibiting Staphylococcus aureus, characterized in that: The drug comprises the following raw materials by weight: 1-10 parts of cloves, 1-10 parts of pygmy grass, 1-5 parts of scutellaria baicalensis, 1-5 parts of scutellaria baicalensis, 1-3 parts of radix paeoniae alba, 1-3 parts of smilax glabra and 1-3 parts of wormwood.

2. The Chinese medicine composition according to claim 1, characterized in that: The raw materials are water extracts of various crude drugs.

3. A method for preparing the Chinese medicine composition according to claim 1 or 2, characterized in that: The following steps are involved: The crude drugs of the raw material medicine are decocted in water to obtain water decoctions, which are concentrated and dried, and then the water extracts of the crude drugs are mixed according to mass parts.

4. The preparation method according to claim 3, characterized in that: The water decoction is performed 2-3 times, with a solid-liquid ratio of 1 g:5-40 mL, and each decoction takes 20-50 minutes.

5. The preparation method according to claim 4, characterized in that: The water decoction is performed three times; the material-liquid ratio of the first water decoction is 1g:5-40mL, and the decoction is performed for 25-35min; the material-liquid ratio of the second and third water decoctions is 1g:10-25mL, and the decoction is performed for 35-45min each time.

6. Use of the traditional Chinese medicine composition according to claim 1 or 2 in preparing a drug for inhibiting Staphylococcus aureus.

7. A drug for inhibiting Staphylococcus aureus, characterized in that: A Chinese medicine composition comprising the Chinese medicine composition according to claim 1 or 2.

8. The drug according to claim 7, characterized in that The content of the traditional Chinese medicine composition is ≥3 mg / mL.

9. The drug according to claim 7, characterized in that The medicine is an external preparation, including ointment, cream, solution, spray, gel, powder, lotion and patch.

10. The drug according to claim 9, characterized in that The medicine also contains pharmaceutically acceptable excipients.

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