Polyglucosamine sodium carboxylate gel as well as preparation method and application thereof

Abstract

The invention relates to the field of biomedical materials, and discloses a sodium glucosamine carboxylate gel which is characterized by comprising the following components in percentage by weight / volume: 1%-2% w / v of sodium hyaluronate, 0.5%-1% w / v of polyacrylic acid, 5%-10% w / v of glycerol, 0.1% w / v of EDTA-2Na, 1%-2% w / v of calcium chloride, 0.5% w / v of sodium carboxymethyl cellulose, 1%-3% w / v of sodium glucosamine carboxylate, 0.5% w / v of PLGA nanoparticles and the balance of purified water. The anti-adhesion hemostatic agent has remarkable hemostatic, wound healing and anti-adhesion effects after endoscopic resection, can be injected and sprayed, and has wide application potential in endoscopic surgery.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical materials, and particularly relates to a sodium polyglucosamine carboxylate gel, a preparation method thereof, and an application thereof. Background Art

[0002] Existing materials for endoscopic hemostasis have shown good hemostatic effects and biocompatibility. For example, rapid hemostasis is achieved through the synergistic effect of chitosan hemostatic powder and sodium hyaluronate, and aero-gel is rapidly formed at the wound using sodium carboxymethylcellulose hydrogel. However, although the antibacterial ability of chitosan is persistent, it may not be sufficient to completely prevent infection in some complex environments. In addition, although this material has certain hemostatic and wound healing effects, its performance in preventing adhesion needs to be improved. Summary of the Invention

[0003] Aiming at the above-mentioned existing technical deficiencies, the technical problem to be solved by the present invention is to provide a sodium polyglucosamine carboxylate gel, a preparation method thereof, and an application thereof, aiming to solve the problems of insufficient antibacterial ability, limited wound healing effect, and poor anti-adhesion performance of existing hemostatic materials after endoscopic resection.

[0004] To solve the above technical problems, the present invention adopts the following technical solutions: The present invention provides a sodium polyglucosamine carboxylate gel, comprising: calculated by weight / volume percentage, the gel contains 1% - 2% w / v of sodium hyaluronate, 0.5% - 1% w / v of polyacrylic acid, 5% - 10% w / v of glycerol, 0.1% w / v of EDTA-2Na, 1% - 2% w / v of calcium chloride, 0.5% w / v of sodium carboxymethylcellulose, 1% - 3% w / v of sodium polyglucosamine carboxylate, 0.5% w / v of PLGA nanoparticles, and the balance is purified water.

[0005] Further, it also includes: 0.01 - 0.1% w / v of nano silver.

[0006] An application of the sodium polyglucosamine carboxylate gel, including applying the sodium polyglucosamine carboxylate gel to hemostasis, wound healing, and anti-adhesion after endoscopic resection.

[0007] A preparation method of the sodium polyglucosamine carboxylate gel, comprising the following steps: S1. Dissolve sodium hyaluronate in purified water, and add polyacrylic acid, glycerol, and EDTA-2Na to form a mixed solution; S2. Add a calcium chloride solution to the mixed solution to form a preliminary cross-linked gel network; S3. Copolymerize sodium carboxymethylcellulose and polyacrylic acid and adjust the phase transition temperature to room temperature to achieve sol-gel phase transition; S4. Ultrasonically disperse sodium polyglucosamine carboxylate and PLGA nanoporous particles in the gel of step S3; S5. Sterilize the prepared gel.

[0008] Furthermore, after the gel is made, it is dried and then nano-crushed into powder particles for spraying.

[0009] The beneficial effects of the present invention are as follows: The present invention has significant hemostatic, wound healing and anti-adhesion effects after endoscopic resection. It can not only be injected but also sprayed, and has broad application potential in endoscopic surgery. Description of the Drawings

[0010] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0011] Figure 1 It is a schematic flow chart of a method for preparing a sodium polyglucosamine carboxylate gel provided by an embodiment of the present invention. Detailed Embodiments

[0012] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention. Embodiment

[0013] This embodiment provides a sodium polyglucosamine carboxylate gel, which is characterized in that, by weight / volume percentage, the gel contains 1% - 2% w / v of sodium hyaluronate, 0.5% - 1% w / v of polyacrylic acid, 5% - 10% w / v of glycerol, 0.1% w / v of EDTA-2Na, 1% - 2% w / v of calcium chloride, 0.5% w / v of sodium carboxymethylcellulose, 1% - 3% w / v of sodium polyglucosamine carboxylate, 0.5% w / v of PLGA nanoparticles, and the balance is purified water. It should be noted that the hemostasis mechanism of this embodiment is that the sodium polyglucosamine carboxylate gel can quickly absorb exudate and form a stable gel layer to achieve the purpose of hemostasis, promote postoperative wound healing, and reduce complications. Because its antibacterial ability is not as strong as that of chitosan, to further enhance the antibacterial ability, 0.01 - 0.1% w / v of silver nanoparticles can be added.

[0014] This embodiment also provides an application of sodium polyglucosamine carboxylate gel, including applying the sodium polyglucosamine carboxylate gel to hemostasis, wound healing and anti-adhesion after endoscopic resection.

[0015] As Figure 1 shown, this embodiment also provides a preparation method of sodium polyglucosamine carboxylate gel, including the following steps: S1. Dissolve sodium hyaluronate in purified water, and add polyacrylic acid, glycerol and EDTA-2Na to form a mixed solution; S2. Add calcium chloride solution to the mixed solution to form a preliminary cross-linked gel network; S3. Copolymerize sodium carboxymethylcellulose and polyacrylic acid and adjust the phase transition temperature to room temperature to achieve sol-gel phase transition; S4. Ultrasonically disperse sodium polyglucosamine carboxylate and PLGA nanoporous particles in the gel; S5. Sterilize the prepared gel. It should be noted that: The sodium polyglucosamine carboxylate gel provided in this embodiment has significant hemostasis, wound healing and anti-adhesion effects after endoscopic resection. Its injectable and powder particle forms, and the characteristics of being able to be made into a gel, dried, and then nano-crushed for spraying make it have broad application potential in endoscopic surgery. Example

[0016] This embodiment is the second embodiment of the present invention. The difference between this embodiment and the first embodiment is that it provides a verification test of a sodium polyglucosamine carboxylate gel, its preparation method and its application, to verify and explain the technical effects adopted in this method.

[0017] In this embodiment, mice are used as experimental subjects. The experimental subjects are randomly divided into two groups. Group A uses the materials of the traditional scheme, and Group B uses the materials of this scheme. The two materials are respectively applied to the wound surface through an endoscope, and observations are made at 0 hour, 6 hours, 24 hours, and 72 hours. The evaluation indicators include hemostasis time, antibacterial effect, wound healing situation and anti-adhesion effect. The results are shown in the following table.

[0018] Table 1: Result comparison table.

[0019] Evaluation index group Hemostasis time Antibacterial effect Wound healing condition Adhesion prevention effect A The average hemostasis time is 10 minutes The diameter of the inhibition zone is 12 mm The healing rate at 72 hours is 85% The adhesion prevention effect is poor B The average hemostasis time is 8 minutes The diameter of the inhibition zone is 11 mm The healing rate at 72 hours is 95% There is an obvious adhesion prevention effect According to Table 1, it can be seen that the method of this embodiment is superior to the traditional method in terms of hemostasis effect, wound healing situation, and especially the anti-adhesion effect of the sodium polyglucosamine carboxylate gel prepared in this embodiment is significantly better than the traditional method.

[0020] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A polyglucosamine carboxylate sodium gel, characterized in that: include: In terms of weight / volume percentage, the gel contains 1%~2%w / v sodium hyaluronate, 0.5%~1%w / v polyacrylic acid, 5%~10%w / v glycerol, 0.1%w / v EDTA-2Na, 1%~2%w / v calcium chloride, 0.5%w / v sodium carboxymethyl cellulose, 1%~3%w / v sodium polyglucosamine carboxylate, 0.5%w / v PLGA nanoparticles, and the remainder is purified water.

2. The polyglucosamine carboxylate sodium gel according to claim 1, characterized in that: Also includes: 0.01~0.1%w / v nanosilver.

3. An application of polyglucosamine carboxylate sodium gel, characterized in that: The polyglucosamine carboxylate sodium gel according to any one of claims 1 to 2 is used for hemostasis, wound healing and adhesion prevention after endoscopic resection.

4. A method for preparing sodium polyglucosamine carboxylate gel, characterized in that: The following steps are involved: S1. Dissolve sodium hyaluronate in purified water, and add polyacrylic acid, glycerol and EDTA-2Na to form a mixed solution; S2, adding calcium chloride solution to the mixed solution to form a preliminary cross-linked gel network; S3, copolymerizing sodium carboxymethyl cellulose and polyacrylic acid and adjusting the phase transition temperature to room temperature to achieve a sol-gel phase transition; S4, ultrasonically dispersing the polyglucosamine carboxylate sodium and the PLGA nanoporous particles in the gel of step S3; S5. Sterilize the prepared gel.

5. The method for preparing the sodium polyglucosamine carboxylate gel according to claim 4, wherein: After being made into gel, it is dried and nano-crushed into powder particles for spraying.

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