Automatic microbial molecule detection equipment

Abstract

The invention relates to the technical field of microbial molecule detection, in particular to automatic microbial molecule detection equipment which comprises a box body, a controller is fixedly connected to the surface of the box body, and a first partition plate and a second partition plate which are used for dividing the interior of the box body into a grinding cavity, a first chamber and a filtering cavity are fixedly connected into the box body; a grinding assembly is arranged in the grinding cavity, and a crushing assembly is arranged in the first cavity; and a filtering assembly is arranged in the filtering cavity. A centrifugal assembly is arranged on the top of the box. A fixing block and a driving part are fixedly connected to the inner top wall of the first cavity, a rotating disc is eccentrically and fixedly connected to an output shaft of the driving part, an electromagnet is fixedly connected into the rotating disc, and an L-shaped connecting rod is hinged to the side wall of the fixing block. By means of the grinding assembly, the crushing assembly, the filtering assembly and the centrifuging assembly, cutting, grinding, centrifuging and filtering treatment of samples is achieved, multiple different types of samples can be detected, and therefore the detection efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial molecular detection, and particularly to an automated microbial molecular detection device. Background Art

[0002] Automated microbial molecular detection devices usually integrate a variety of high-tech technologies, such as molecular biology, automation technology, computer technology, etc., to achieve rapid processing and accurate detection of microbial samples. These devices can automatically complete multiple steps such as sample preparation, extraction, amplification, detection, and result analysis, greatly improving the detection efficiency and accuracy.

[0003] Generally speaking, molecular detection devices need to process various types of samples and quickly and accurately detect biological samples respectively. However, in the prior art, most devices are limited to detecting biological specimens, and sample pretreatment mostly needs to be completed manually, which limits the scope of application. Some devices are only for specific biological specimens (such as blood) and lack the ability to process various types of samples (such as environmental and food samples).

[0004] In summary, how to solve the problem of the lack of the ability to process various types of samples in existing devices has become a technical problem that needs to be urgently solved by those skilled in the art. Therefore, it is necessary to propose an automated microbial molecular detection device. Summary of the Invention

[0005] To solve the above problems, the present invention provides an automated microbial molecular detection device, which realizes cutting, grinding, centrifuging, and filtering of samples through a grinding component, a crushing component, a filtering component, and a centrifuging component, and can detect various different types of samples, thereby improving the detection efficiency.

[0006] To achieve the above object, the technical solution of the present invention is as follows: an automated microbial molecular detection device, including a box body, and further including a detection system, and a controller is fixedly connected to the surface of the box body.

[0007] A first partition plate is fixedly connected inside the box body for dividing the inside of the box body into a first chamber and a second chamber from left to right in sequence. A second partition plate in an L shape for dividing the second chamber into a grinding chamber and a filtering chamber is arranged in the second chamber. The vertical end of the second partition plate is fixedly connected to the inner top wall of the box body, and the horizontal end of the second partition plate is fixedly connected to the inner side wall of the box body and the first partition plate.

[0008] Feeding ports and discharging ports are respectively opened on the top walls and side walls of the first chamber, the grinding chamber, and the filtering chamber. Buckle covers are detachably connected inside the feeding ports and the discharging ports.

[0009] The grinding chamber is provided with a grinding assembly for grinding biological specimens, the first chamber is provided with a crushing assembly for crushing biological specimens; the filtering chamber is provided with a filtering assembly for filtering biological specimens.

[0010] The top of the box body is provided with a centrifugation assembly for centrifuging biological specimens.

[0011] The inner top wall of the first chamber is fixedly connected with a fixed block and a driving member. The output shaft of the driving member is eccentrically fixedly connected with a rotating disk. An electromagnet is fixedly connected inside the rotating disk. The side wall of the fixed block is hinged with an L-shaped connecting rod. One end of the connecting rod contacts the rotating disk, and the other end of the connecting rod penetrates through the first partition plate and extends into the grinding chamber to be fixedly connected with the grinding assembly; the crushing assembly is fixedly connected to the bottom of the rotating disk.

[0012] The controller is used to control the electromagnet to attract the connecting rod, and the controller is used to control the driving member to drive the rotating disk to rotate.

[0013] The technical principle of the above solution is as follows: The driving member drives the rotating disk to rotate. The electromagnet inside the rotating disk and the eccentric rotation of the rotating disk drive the connecting rod to move, so that the moving rod always abuts against the rotating disk. The rotation of the rotating disk and the movement of the connecting rod can drive the crushing assembly and the grinding assembly to operate respectively, so as to realize the crushing and grinding of the sample.

[0014] Through the grinding action of the grinding assembly, the sample is cut and ground into fine particles; through the cutting and crushing of the crushing assembly, the sample will be fully cut; through the filtering action of the filtering assembly, impurities and larger particles can be removed to obtain a purer sample; through the centrifugation of the centrifugation assembly on the sample, molecular detection work can be facilitated.

[0015] Adopting the above solution has the following beneficial effects:

[0016] 1. The present invention realizes the detection of various different types of samples through the grinding assembly, the crushing assembly, the filtering assembly and the centrifugation assembly, thereby improving the detection efficiency of the samples. Compared with traditional sample detection, the device can complete the pretreatment of more samples in a shorter time, thus significantly improving the time efficiency of detection.

[0017] 2. The present invention detects various different types of samples, which reduces the waiting time of the staff while improving the detection efficiency, thereby reducing the labor cost.

[0018] 3. The crushing assembly and the grinding assembly of the present invention are linked through a connecting rod. When the driving member is started, the rotating disk drives the connecting rod to move, the rotating disk drives the crushing assembly to operate, and the connecting rod drives the grinding assembly to operate. This design reduces the time and resources required to control the two components separately and improves the overall processing efficiency.

[0019] Further, the grinding assembly includes a grinding block, which is fixedly connected to the connecting rod; a limiting block is fixedly connected to the top wall of the grinding cavity, and the grinding block is located on one side of the limiting block and is slidably matched with the limiting block.

[0020] Beneficial effects: Through the sliding fit between the limiting block and the grinding block, it can ensure that the grinding block slides stably along a predetermined trajectory in the grinding cavity, thereby reducing the shaking or deviation during the grinding process, and thus ensuring the uniformity and consistency of the grinding effect. The grinding block is driven by the connecting rod to perform grinding, enabling the grinding block to efficiently grind the sample.

[0021] Further, the crushing assembly includes a crushing rod, which is located at the bottom of the rotating disk and is fixedly connected to the output shaft of the driving member, and a plurality of crushing blades are fixedly connected to the crushing rod.

[0022] Beneficial effects: The crushing rod is fixedly connected to the output shaft of the driving member, ensuring that the crushing rod can rotate stably as the driving member rotates. This stable rotational motion, combined with the crushing blades fixedly connected to the crushing rod, can generate a powerful crushing force, effectively crushing the sample into smaller particles and improving the crushing efficiency.

[0023] Further, a plurality of stirring blades are also fixedly connected to the crushing rod.

[0024] Beneficial effects: The stirring blades will drive the surrounding biological specimens to mix when the crushing rod rotates, ensuring that the biological specimens are fully stirred during the crushing process. The presence of the stirring blades increases the contact area between the crushing assembly and the biological specimens, thereby improving the crushing efficiency. During the crushing process, the stirring blades can not only assist the crushing blades in crushing, but also push larger particles towards the crushing blades, making them easier to be crushed.

[0025] Further, the filtering assembly includes a filtering box, which is fixedly connected to the bottom wall of the filtering cavity. A filtering membrane is fixedly connected inside the filtering box. The filtering box is communicated with a suction assembly for providing negative pressure suction for the biological specimens in the filtering box. The top wall of the filtering box is communicated with the feeding port of the filtering cavity, and the side wall of the filtering box is communicated with the discharging port of the filtering cavity.

[0026] Beneficial effects: The filtering box filters out impurities and particulate matters in the biological specimens through the filtering membrane and the suction assembly, which is beneficial to improving the purity of the samples in the filtering box. By fixedly connecting the filtering box to the bottom wall of the filtering cavity, the stability and reliability of the filtering process are ensured. And through the suction assembly, the filtering box can continuously and efficiently filter the samples, further improving the filtering efficiency.

[0027] Further, the suction assembly includes a pump assembly which is fixedly connected to the side wall of the box body. The air inlet end of the pump assembly is located below the filter membrane and communicates with the filter box. A filter plate is fixedly connected at the connection between the air inlet end of the pump assembly and the filter box. The controller is used to control the pump assembly to start and filter the sample.

[0028] Beneficial effects: The pump assembly is fixedly connected to the side wall of the box body, ensuring the stability and reliability of the suction process. The pump assembly can work continuously and efficiently to filter the impurities in the sample in the filter box, improving the filtration efficiency of the filter box. The air inlet end of the pump assembly is located below the filter membrane and communicates with the filter box, ensuring the smooth separation of impurities in the sample from the sample, thereby improving the purity of the sample.

[0029] Further, the centrifugation assembly includes a centrifuge disk. The output shaft at the end of the driving member away from the rotating disk penetrates through the box body and is fixedly connected to the centrifuge disk. A plurality of test tube racks are fixedly connected to the centrifuge disk.

[0030] Beneficial effects: The centrifuge disk is fixedly connected to the output shaft of the driving member, ensuring the stability and reliability of the centrifuge disk during rotation. This design reduces vibration and noise during centrifugation and improves the running smoothness of the equipment. The test tube racks are fixedly connected to the centrifuge disk, which can accommodate multiple test tubes and can adjust the position and quantity of the test tubes as needed. This design enables the equipment to be applicable to the processing of samples of different sizes and quantities, improving the flexibility and versatility of the equipment.

[0031] Further, a spring is fixedly connected to one end of the grinding block close to the connecting rod, and the end of the spring away from the grinding block is fixedly connected to the limiting block.

[0032] Beneficial effects: As an elastic element, the spring has good buffering and shock-absorbing performance. During the grinding process, due to the contact force and friction force between the grinding block and the object to be ground, vibrations and impacts may occur. The presence of the spring can absorb and disperse these vibrations and impacts, protecting the grinding block and the connecting rod from damage, and at the same time improving the smoothness and stability of the grinding process.

[0033] Further, the detection system includes a detection module which uses the fluorescence quantitative polymerase chain reaction technology to detect and quantitatively analyze the sample, and detects the sample according to the reaction temperature, time, primer concentration and fluorescent dye in the real-time fluorescence quantitative polymerase chain reaction.

[0034] Beneficial effects: The real-time fluorescence quantitative polymerase chain reaction technology has high sensitivity and can detect DNA at extremely low concentrations. By optimizing the reaction conditions, the sensitivity of the detection can be further improved to ensure accurate detection even when the content of the target DNA in the sample is extremely low.

[0035] Furthermore, the detection system further includes an adjustment module, which is used to adjust the reaction temperature, time, primer concentration, and fluorescent dye in real-time fluorescence quantitative polymerase chain reaction.

[0036] Beneficial effects: The adjustment module allows users to flexibly adjust the reaction conditions of real-time fluorescence quantitative polymerase chain reaction according to specific experimental requirements and sample characteristics. This flexibility enables the detection system to be applicable to a variety of different samples and detection targets, improving the adaptability and application scope of the system.

[0037] Additional aspects and advantages of the present invention will be given in part in the following description, become apparent in part from the following description, or be learned through the practice of the present invention. Brief Description of the Drawings

[0038] Figure 1 Isometric view of the automated microbial molecular detection device of the present invention.

[0039] Figure 2 Internal isometric view of the automated microbial molecular detection device of the present invention.

[0040] Figure 3 Isometric view of the grinding assembly of the automated microbial molecular detection device of the present invention.

[0041] Figure 4 For the present invention Figure 1 Cross-sectional view taken along line A-A in

[0042] Figure 5 For the present invention Figure 1 Cross-sectional view taken along line B-B in

[0043] Figure 6 Structural block diagram of the detection system of the automated microbial molecular detection device of the present invention.

[0044] Reference numerals in the drawings of the specification include: 1, box body; 2, centrifugal disk; 3, test tube rack; 4, feed inlet; 5, discharge outlet; 6, negative pressure pump; 7, double-headed motor; 8, rotating disk; 9, connecting rod; 10, stirring blade; 11, crushing blade; 12, crushing rod; 13, grinding block; 14, limiting block; 15, filter box; 16, spring; 17, first chamber; 18, first partition board; 19, filtration chamber; 20, second partition board; 21, grinding chamber. Detailed Description of the Specific Embodiments

[0045] The following is a more detailed description through specific embodiments:

[0046] Embodiment 1:

[0047] As shown in the attached Figures 1 - 5As shown: An automated microbial molecular detection device, including a box body 1, also includes a detection system, and a controller is fixedly connected to the surface of the box body 1 by screws.

[0048] A first partition plate 18 for dividing the interior of the box body 1 into a first chamber 17 and a second chamber in sequence from left to right is fixedly connected to the box body 1 by bolts. A second partition plate 20 in an L shape for dividing the second chamber into a grinding chamber 21 and a filtering chamber 19 is provided in the second chamber. The vertical end of the second partition plate 20 is fixedly connected to the inner top wall of the box body 1 by bolts, and the horizontal end of the second partition plate 20 is fixedly connected to the inner side wall of the box body 1 and the first partition plate 18 by bolts.

[0049] Feeding ports 4 and discharging ports 5 are respectively opened on the top walls and side walls of the first chamber 17, the grinding chamber 21, and the filtering chamber 19. Cover caps are detachably connected to the feeding ports 4 and the discharging ports 5.

[0050] A grinding assembly for grinding biological specimens is provided in the grinding chamber 21, a crushing assembly for crushing biological specimens is provided in the first chamber 17, and a filtering assembly for filtering biological specimens is provided in the filtering chamber 19.

[0051] A centrifugal assembly for centrifuging biological specimens is provided on the top of the box body 1.

[0052] A fixing block and a driving member are fixedly connected to the inner top wall of the first chamber 17. In this embodiment, the driving member is preferably a double-headed motor 7. The output shaft at the bottom of the double-headed motor 7 is fixedly connected to a rotating disk 8 by an eccentric bolt. An electromagnet is fixedly connected to the rotating disk 8 by screws. An L-shaped connecting rod 9 is hinged to the side wall of the fixing block. One end of the connecting rod 9 contacts the rotating disk 8, and the other end of the connecting rod 9 penetrates through the first partition plate 18 and extends into the grinding chamber 21 and is fixedly connected to the grinding assembly by bolts; the crushing assembly is fixedly connected to the bottom of the rotating disk 8.

[0053] The controller is used to control the electromagnet to attract the connecting rod 9, and the controller is used to control the double-headed motor 7 to drive the rotating disk 8 to rotate.

[0054] As Figure 2 shown, the grinding assembly includes a grinding block 13, and the grinding block 13 is fixedly connected to the connecting rod 9 by bolts; a limiting block 14 is fixedly connected to the inner top wall of the grinding chamber 21, and the grinding block 13 is located on one side of the limiting block 14 and is in sliding fit with the limiting block 14.

[0055] The crushing assembly includes a crushing rod 12. The crushing rod 12 is located at the bottom of the rotating disk 8 and is fixedly connected to the output shaft of the double-headed motor 7. A plurality of crushing blades 11 are fixedly connected to the crushing rod 12.

[0056] As Figure 5As shown, the filtering component includes a filtering box 15, which is bolted to the inner bottom wall of the filtering chamber 19. A filtering membrane is fixedly connected to the filtering box 15 by screws. The filtering box 15 is connected to a suction component for providing negative pressure suction to the biological sample in the filtering box 15. The top wall of the filtering box 15 is connected to the feeding port 4 of the filtering chamber 19, and the side wall of the filtering box 15 is connected to the discharging port 5 of the filtering chamber 19.

[0057] The suction component includes a pump component. In this embodiment, the pump component is preferably a negative pressure pump 6, which is bolted to the side wall of the box body 1. The air inlet end of the negative pressure pump 6 is located below the filtering membrane and is connected to the filtering box 15. A filtering plate is bolted to the connection between the air inlet end of the negative pressure pump 6 and the filtering box 15. The controller is used to control the start of the negative pressure pump 6 to filter the sample.

[0058] As Figure 2 shown, the centrifugation component includes a centrifugal disk 2. The output shaft at the top of the double-headed motor 7 passes through the box body 1 and is bolted to the centrifugal disk 2. A number of test tube racks 3 are fixedly connected to the centrifugal disk 2 by screws.

[0059] The specific implementation process is as follows: First, the staff can determine the components to be used according to the type of the sample.

[0060] Taking Figure 2 and Figure 4 as an example, when the sample to be detected needs to use the grinding component, first, the sample can be put into the grinding chamber 21 through the feeding port 4 at the top of the grinding component. Then, the double-headed motor 7 and the electromagnet can be started through the controller. The rotating disk 8 is driven by the double-headed motor 7 to rotate eccentrically. When the rotating disk 8 rotates eccentrically, the connecting rod 9 is continuously attracted by the electromagnet, so that the rotating disk 8 always abuts against the connecting rod 9. The rotation of the rotating disk 8 drives the connecting rod 9 to move reciprocally continuously. When the connecting rod 9 moves reciprocally, it will drive the grinding block 13 to move reciprocally continuously within the limiting block 14, thereby achieving the effect of continuously grinding the sample. After the grinding by the grinding block 13 is completed, the staff can take out the ground sample through the discharging port 5 of the grinding chamber 21. The biological sample is ground by the grinding block 13 to make it finer into smaller particles, which is convenient for the subsequent processing and analysis.

[0061] When the sample to be detected needs to use the crushing component, the sample can be put into the first chamber 17 through the feeding port 4 at the top of the first chamber 17. Subsequently, the double-headed motor 7 is started through the controller, and the crushing rod 12 is driven to rotate by the double-headed motor 7. When the crushing rod 12 rotates, the crushing blade 11 will be driven to rotate, and the sample is crushed by the crushing rod 12 and the crushing blade 11. After the sample is crushed, the staff can take out the sample through the discharge port 5 of the first chamber 17. By stably crushing the sample, it is beneficial to ensure the uniformity and consistency of the sample. And it can improve the efficiency of sample crushing.

[0062] Combined Figure 5 As shown, when the sample to be detected needs to be filtered and centrifuged, first, the staff needs to put the sample into the filter box 15 through the feeding port 4 of the filtering chamber 19. Subsequently, the staff can start the negative pressure pump 6 through the controller, and use the negative pressure suction generated by the negative pressure pump 6 to filter the sample in the filter box 15. When the negative pressure pump 6 is started, the sample above the filter membrane will pass through the filter membrane under the action of the negative pressure suction. In this embodiment, the filtering diameter of the filter plate is selected to be smaller than the filtering diameter of the filter membrane, so that the sample in the filter box 15 cannot enter the negative pressure pump 6 through the filter plate. And the staff can adjust the power of the negative pressure pump 6 through the controller to reduce the negative pressure suction of the negative pressure pump 6, so that the sample will fall to the bottom of the filter box 15 under the action of gravity after passing through the filter membrane. Therefore, through the filtering and blocking effect of the filter plate and adjusting the negative pressure suction of the negative pressure pump 6, the sample will smoothly enter the bottom of the filter box 15 when passing through the filter membrane and will not enter the negative pressure pump 6 through the filter plate, while the impurities with larger particles in the sample will stay above the filter membrane under the blocking effect of the filter membrane. At this time, the impurities in the sample will be continuously separated under the action of the negative pressure pump 6, so as to achieve the effect of filtering the sample. When the sample filtering is completed, the sample can be taken out through the discharge port 5 communicated with the filtering chamber 19.

[0063] Subsequently, the staff can put the filtered sample into the test tube rack 3 through the test tube. At this time, the staff can open the double-headed motor 7 through the controller, and use the double-headed motor 7 to drive the centrifuge disk 2 to rotate, and centrifuge the test tubes in the test tube rack 3 through the rotation of the centrifuge disk 2. By centrifuging the sample, different components in the sample are separated, which is convenient for subsequent analysis and research. By centrifuging, it is beneficial to quickly separate the components in the sample, reduce the subsequent processing time, and thus improve the detection efficiency.

[0064] By integrating the grinding component, crushing component, filtering component and centrifugal component within the box body 1, staff can easily process various types of samples, improving the processing efficiency of multiple types of samples by staff and reducing the labor costs incurred by staff during sample processing. Moreover, during sample processing, staff can perform connection processing among the grinding component, crushing component, filtering component and centrifugal component, reducing the risk of sample contamination that exists when transferring samples between different devices.

[0065] During the processing of multiple types of samples, staff can process different types of samples simultaneously. For example, during the centrifugation of blood samples, staff can simultaneously grind and crush food samples, thereby further improving the processing efficiency of multiple samples by staff.

[0066] By separately processing different types of samples, the mutual interference between different types of samples is also reduced, which is conducive to improving the accuracy of analysis results.

[0067] Embodiment 2:

[0068] As Figure 2 shown, the difference from the above embodiment is that a number of stirring blades 10 are also fixedly connected to the crushing rod 12 by bolts.

[0069] The specific implementation process is as follows: During the rotation of the stirring blade 10, the sample can be continuously stirred, enabling different components in the sample to be fully mixed. This mixing effect helps to ensure the uniformity of the sample and reduce detection errors caused by uneven sample distribution. The stirring by the stirring blade 10 is also conducive to increasing the mutual contact between the sample and the crushing blade 11, thereby improving the crushing efficiency of the sample.

[0070] Embodiment 3:

[0071] As Figure 3 shown, the difference from the above embodiment is that a spring 16 is fixedly connected to the left end of the grinding block 13 by screws, and the right end of the spring 16 is fixedly connected to the limit block 14 by screws.

[0072] The specific implementation process is as follows: The spring 16 has elasticity. When the grinding block 13 slides leftward within the limit block 14, the spring 16 is compressed. When the grinding block 13 slides rightward, the elastic force by which the spring 16 is compressed will be released, thereby pushing the grinding block 13 to slide rightward within the limit block 14 faster, thus improving the grinding efficiency of the sample within the grinding chamber 21.

[0073] During the grinding process of the grinding block 13, when the grinding block 13 slides to the left, the spring 16 will be continuously compressed. The elastic force of the spring 16 itself can reduce the direct impact between the grinding block 13 and the limit block 14, thereby increasing the service life of the grinding block 13.

[0074] Embodiment 4:

[0075] As Figure 6 shown, the difference from the above embodiment is that the detection system includes a detection module. The detection module uses the fluorescence quantitative polymerase chain reaction technology to detect and quantitatively analyze the sample, and detects the sample according to the reaction temperature, time, primer concentration, and fluorescent dye in the real-time fluorescence quantitative polymerase chain reaction.

[0076] The specific implementation process is as follows: When detecting and quantitatively analyzing the sample through the detection module, the fluorescence quantitative polymerase chain reaction technology can achieve precise detection of the target gene in trace samples, has high sensitivity, and can also provide quantitative information on the absolute concentration or relative expression level of the target gene in the sample. This helps to make more precise comparisons and analyses of the samples.

[0077] Embodiment 5:

[0078] As Figure 6 shown, the difference from the above embodiment is that the detection system further includes an adjustment module, and the adjustment module is used to adjust the reaction temperature, time, primer concentration, and fluorescent dye in the real-time fluorescence quantitative polymerase chain reaction.

[0079] The specific implementation process is as follows: By adjusting the reaction temperature, time, and primer concentration, it is possible to ensure high amplification efficiency and specificity of the target sequence. This helps to reduce the occurrence of non-specific amplification and false positive results, and improve the accuracy and reliability of the experiment. The adjustment module allows the staff to perform personalized settings on the reaction conditions of the real-time fluorescence quantitative polymerase chain reaction according to specific experimental requirements and sample characteristics. This helps to achieve the best experimental results and improve the sensitivity of the experiment.

[0080] Obviously, the above embodiments are merely examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all the implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. An automated microbial molecular detection device, comprising a housing (1), characterized in that: Also includes detection systems; A controller is fixedly connected to the surface of the box (1); A first partition plate (18) is fixedly connected inside the box body (1) and is used to divide the inside of the box body (1) into a first chamber (17) and a second chamber from left to right in sequence; an L-shaped second partition plate (20) is provided inside the second chamber and is used to divide the second chamber into a grinding chamber (21) and a filtering chamber (19); the vertical end of the second partition plate (20) is fixedly connected to the top wall of the box body (1), and the horizontal end of the second partition plate (20) is fixedly connected to the inner side wall of the box body (1) and the first partition plate (18); The top wall and side wall of the first chamber (17), the grinding chamber (21) and the filtering chamber (19) are respectively provided with an inlet (4) and an outlet (5), and buckle covers are detachably connected to the inlet (4) and the outlet (5); A grinding assembly for grinding biological samples is provided in the grinding chamber (21); The first chamber (17) is provided with a crushing assembly for crushing the biological sample; A filter assembly for filtering biological samples is arranged in the filter cavity (19); A centrifugal assembly for centrifuging biological samples is provided on the top of the box (1); The inner top wall of the first chamber (17) is fixedly connected with a fixed block and a driving member; the output shaft of the driving member is eccentrically fixedly connected with a rotating disk (8), and an electromagnet is fixedly connected inside the rotating disk (8); an L-shaped connecting rod (9) is hingedly connected to the side wall of the fixed block, one end of the connecting rod (9) is in contact with the rotating disk (8), and the other end of the connecting rod (9) passes through the first partition plate (18) and extends into the grinding chamber (21) to be fixedly connected with the grinding assembly; The crushing assembly is fixedly connected to the bottom of the rotating disk (8); The controller is used to control the electromagnet to start attracting the connecting rod (9); the controller is used to control the driving member to drive the rotating disk (8) to rotate.

2. The automated microbial molecular detection device according to claim 1, characterized in that: The grinding assembly comprises a grinding block (13), and the grinding block (13) is fixedly connected to a connecting rod (9); The inner top wall of the grinding chamber (21) is fixedly connected to a limiting block (14); the grinding block (13) is located on one side of the limiting block (14) and is slidably matched with the limiting block (14).

3. The automated microbial molecular detection device according to claim 2, characterized in that: The crushing assembly comprises a crushing rod (12), which is located at the bottom of the rotating disk (8) and fixedly connected to the output shaft of the driving member, and a plurality of crushing blades (11) are fixedly connected to the crushing rod (12).

4. The automated microbial molecular detection device according to claim 3, characterized in that: A plurality of stirring blades (10) are also fixedly connected to the crushing rod (12).

5. The automated microbial molecular detection device according to claim 4, characterized in that: The filter assembly comprises a filter box (15), the filter box (15) is fixedly connected to the inner bottom wall of the filter cavity (19), and a filter membrane is fixedly connected inside the filter box (15); The filter box (15) is connected to a suction assembly for providing negative pressure suction for the biological sample in the filter box (15); The top wall of the filter box (15) is in communication with the inlet (4) of the filter chamber (19), and the side wall of the filter box (15) is in communication with the outlet (5) of the filter chamber (19).

6. The automated microbial molecular detection device according to claim 5, characterized in that: The suction assembly comprises a pump assembly, the pump assembly is fixedly connected to the side wall of the box body (1), the air inlet end of the pump assembly is located below the filter membrane and is connected to the filter box (15), a filter plate is fixedly connected to the connection between the air inlet end of the pump assembly and the filter box (15), and the controller is used to control the pump assembly to start filtering the sample.

7. The automated microbial molecular detection device according to claim 6, characterized in that: The centrifugal assembly comprises a centrifugal disc (2), an output shaft of a driving member at one end away from a rotating disc (8) passes through a housing (1) and is fixedly connected to the centrifugal disc (2), and a plurality of test tube racks (3) are fixedly connected to the centrifugal disc (2).

8. The automated microbial molecular detection device according to claim 7, characterized in that: One end of the grinding block (13) close to the connecting rod (9) is fixedly connected to a spring (16), and one end of the spring (16) away from the grinding block (13) is fixedly connected to a limit block (14).

9. The automated microbial molecular detection device according to claim 8, characterized in that: The detection system includes a detection module; The detection module uses fluorescent quantitative polymerase chain reaction technology to detect and quantitatively analyze samples, and detects samples based on the reaction temperature, time, primer concentration and fluorescent dye in real-time fluorescent quantitative polymerase chain reaction.

10. The automated microbial molecular detection device according to claim 9, characterized in that: The detection system also includes an adjustment module; The adjustment module is used to adjust the reaction temperature, time, primer concentration and fluorescent dye in the real-time fluorescent quantitative polymerase chain reaction.

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