Repair composition suitable for sensitive skin and application thereof

Abstract

The invention provides a repairing composition suitable for sensitive skin and application thereof, and belongs to the technical field of cosmetics, the repairing composition suitable for sensitive skin comprises sparassis crispa extract, lactobacillus / soybean milk fermentation product filtrate, orange fruit extract and hexapeptide-1; wherein the sparassis crispa extract is prepared by taking birch juice, a purslane extract, a corn extract, hydrolyzed protein peptone, trehalose and centella asiatica powder as a culture medium through special fermentation; the obtained sparassis crispa extract is used together with other raw materials in the composition, has a remarkable repairing effect, is mild, sensitive and non-irritant, and is suitable for sensitive skin.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a repair composition suitable for sensitive skin and its application. Background Art

[0002] Skin sensitivity problems are mainly manifested as impaired barrier function, abnormal release of inflammatory factors, and hyperactive nerve reactivity. Currently, cosmetics for sensitive skin repair mostly focus on ceramide supplementation or the application of single anti-inflammatory ingredients (such as bisabolol, dipotassium glycyrrhizinate), but there are the following technical bottlenecks: Single efficacy of active ingredients: Traditional repair ingredients often only target the repair of the physical structure of the barrier (such as ceramide) or inflammation inhibition (such as TNF-α antagonists), lacking the synergistic intervention of multiple pathways of "barrier reconstruction - immune regulation - nerve soothing".

[0003] Stimulation risk of the formulation system: Commercially available sensitive skin products often add high concentrations of polyols (such as 1,2 - hexanediol ≥ 5%) to enhance the immediate soothing effect. Although it can temporarily reduce the sensitivity of TRPV1 receptors, long-term use will disrupt the skin microecological balance.

[0004] Therefore, developing a sensitive skin repair system that combines efficient barrier repair, inflammation regulation, and is safe and mild has become a problem that urgently needs to be solved in the technical field of cosmetics. The present invention has successfully achieved a leapfrog upgrade of sensitive skin repair technology by constructing a specific medium for Sparassis crispa, innovating the fermentation process, and designing a multi-pathway synergistic formulation. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a repair composition suitable for sensitive skin and its application. The components of the repair composition suitable for sensitive skin synergistically enhance the effect, can effectively promote skin barrier repair, enhance cell migration rate, and are mild and non-irritating, suitable for sensitive skin.

[0006] To achieve the above purpose, the present invention discloses the following technical solutions: In the first aspect, the present invention provides a liquid medium suitable for Sparassis crispa, and the medium comprises the following components in mass percentage: birch sap 5 - 15%, purslane extract 0.5 - 3.0%, corn extract 0.5 - 1.5%, hydrolyzed peptone 0.3 - 2.0%, trehalose 1.1 - 1.8%, asiatic pennywort powder 0.01 - 0.1%, and the balance is deionized water; the pH value of the medium is 5.8 - 6.5.

[0007] In the second aspect, the present invention provides a method for preparing Sparassis crispa extract, which is characterized by comprising the following steps: a) Inoculate Sparassis crispa into PDA liquid medium and culture it at 25 - 28 °C in the dark until the OD600 reaches 1.5 ± 0.2 to obtain a bacterial strain solution; b) Take the bacterial strain solution and inoculate it into the Sparassis crispa liquid medium described in the first aspect at an inoculation amount of 10 - 12 v / v%, and culture it by shaking at 150 - 200 rpm and 26 - 30 °C for 10 - 15 days; c) Perform ultrasonic fragmentation treatment on the culture solution, with an ultrasonic frequency of 30 kHz and an ultrasonic time of 20 minutes, then enzymatically hydrolyze it with cellulase for 2 h, and centrifuge to obtain the supernatant. The addition amount of cellulase accounts for 4 - 5 wt% of the culture solution, and the cellulase activity concentration is 20,000 U / g; d) Use an ultrafiltration membrane to retain the active substances in the supernatant with a molecular weight cut-off range of 0 - 10 kDa, and perform freeze-drying on the retained fraction to obtain a Sparassis crispa extract; Among them, the Sparassis crispa is Sparassis crispa, purchased from Ningbo Mingzhou Biology, product number: BMZ126467.

[0008] In the third aspect, the present invention provides an application of the Sparassis crispa extract prepared by the preparation method of the Sparassis crispa extract described in the second aspect in the preparation of a cosmetic with a repair effect. The cosmetic is selected from any one of essence, cream, eye cream, and facial mask, and the addition amount of the Sparassis crispa extract in the cosmetic is 0.5 - 10 wt%.

[0009] In the fourth aspect, the present invention provides a repair composition suitable for sensitive skin, comprising the following components in mass percentage: 4 - 6% of the Sparassis crispa extract prepared by the preparation method of the Sparassis crispa extract described in the second aspect, 0.5 - 1.5% of the filtrate of Lactobacillus / soybean milk fermentation product, 0.5 - 1.5% of orange fruit extract, 0.1 - 0.3% of hexapeptide-1, and the balance is deionized water.

[0010] Advantages of the present invention: Through a unique culture medium formula, Sparassis crispa fermentation process, and active ingredient combination, the present invention realizes a synergistic effect for the repair of sensitive skin. The specific beneficial effects are as follows: Targeted synergistic effect of bacterial source active substances Use the culture medium defined in the present invention to replace the traditional glucose-based culture medium: Polysaccharides and amino acids in birch sap induce Sparassis crispa to secrete small molecule repair peptides rich in proline and glycine, enhancing the affinity with skin keratin; As a signal molecule, asiaticoside derivative activates the secondary metabolic pathway of mycelium, promotes the synthesis of triterpenoids, and synergistically enhances the anti-inflammatory activity.

[0011] Long-term regulation of inhibiting inflammatory factors Synergistic effect of Hexapeptide-1 and orange fruit extract: Hexapeptide-1 inhibits the release of substance P and blocks the conduction pathway of neurogenic inflammation; Limonoids in orange fruit regulate the activity of transient receptor potential (TRPV1) channels and reduce nerve sensitivity reactions such as burning and stinging.

[0012] Through the whole-chain innovative design of strain culture - active extraction - formula synergy, the present invention breaks through the limitation of the single-pathway effect of traditional sensitive skin repair products, realizes the unity of high-efficiency repair and mildness, and is applicable to the sensitive skin state. Detailed implementation manners

[0013] The technical solutions in the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0014] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only used to explain the present invention and are not used to limit the present invention.

[0015] The sources of some raw materials in the present invention are as follows:

[0016] Raw materials and their mass percentages of Sparassis crispa liquid medium: Sparassis crispa liquid medium 1: birch sap 10wt%, purslane extract 1.7wt%, corn extract 1wt%, hydrolyzed peptone 1.3wt%, trehalose 1.5wt%, asiatic pennywort powder 0.05wt%, and the balance is deionized water; the pH value of the medium is 6.0.

[0017] Sparassis crispa liquid medium 2: birch sap 15wt%, purslane extract 3.0wt%, corn extract 0.5wt%, hydrolyzed peptone 0.3wt%, trehalose 1.8wt%, asiatic pennywort powder 0.01wt%, and the balance is deionized water; the pH value of the medium is 5.8.

[0018] Sparassis crispa liquid medium 3: birch sap 5wt%, purslane extract 0.5wt%, corn extract 1.5wt%, hydrolyzed peptone 2.0wt%, trehalose 1.1wt%, asiatic pennywort powder 0.1wt%, and the balance is deionized water; the pH value of the medium is 6.5.

[0019] Sparassis crispa liquid medium ①: Compared with medium 1, it lacks birch sap, and the lacking part is supplemented with purslane extract, corn extract, hydrolyzed peptone, trehalose, and centella asiatica powder in a mass ratio of 1.7:1:1.3:1.5:0.05 by mass percentage.

[0020] Sparassis crispa liquid medium ②: Compared with medium 1, it lacks purslane extract, and the lacking part is supplemented with birch sap, corn extract, hydrolyzed peptone, trehalose, and centella asiatica powder in a mass ratio of 10:1:1.3:1.5:0.05 by mass percentage.

[0021] Sparassis crispa liquid medium ③: Compared with medium 1, it lacks corn extract, and the lacking part is supplemented with birch sap, purslane extract, hydrolyzed peptone, trehalose, and centella asiatica powder in a mass ratio of 10:1.7:1.3:1.5:0.05 by mass percentage.

[0022] Sparassis crispa liquid medium ④: Compared with medium 1, it lacks centella asiatica powder, and the lacking part is supplemented with birch sap, purslane extract, corn extract, hydrolyzed peptone, and trehalose in a mass ratio of 10:1.7:1:1.3:1.5 by mass percentage.

[0023] Configuration steps of Sparassis crispa liquid medium: Mix the raw materials evenly with water, then subpackage and sterilize by high-pressure steam for later use.

[0024] Preparation of Sparassis crispa extract: Sparassis crispa extract 1: a) Inoculate Sparassis crispa into PDA liquid medium and culture it at 27 °C in the dark until OD600 reaches 1.5 to obtain a strain solution; b) Take the strain solution and inoculate it into Sparassis crispa liquid medium 1 at an inoculation amount of 11 v / v%, and culture it by shaking at 175 rpm and 28 °C for 13 days to obtain a culture solution; c) Perform ultrasonic fragmentation treatment on the culture solution, ultrasonic frequency: 30 kHz, ultrasonic time: 20 minutes, then use cellulase for enzymatic hydrolysis for 2 h, centrifuge and take the supernatant, where the addition amount of cellulase accounts for 5 wt% of the culture solution, and the cellulase activity concentration: 20,000 U / g; d) Use an ultrafiltration membrane to retain the active substances in the supernatant with a molecular weight cut-off of 0 - 10 kDa, and perform freeze-drying on the retained fraction to obtain Sparassis crispa extract; Sparassis crispa extract 2: a) Inoculate Sparassis crispa into PDA liquid medium and culture it at 28 °C in the dark until OD600 reaches 1.3 to obtain a strain solution; b) Take the strain solution and inoculate it into Sparassis crispa liquid medium 1 at an inoculation amount of 10 v / v%, and culture it by shaking at 200 rpm and 26 - 30 °C for 10 days to obtain a culture solution; c) Subject the culture medium to ultrasonic disruption treatment with an ultrasonic frequency of 20 kHz and an ultrasonic time of 30 minutes. Then, enzymatically hydrolyze it with cellulase for 2 h, centrifuge to obtain the supernatant. The addition amount of cellulase accounts for 5 wt% of the culture medium, and the cellulase activity concentration is 20,000 U / g; d) Use an ultrafiltration membrane with a molecular weight cut-off range of 0 - 10 kDa to retain the active substances in the supernatant, and perform freeze-drying on the retained fraction to obtain Sparassis crispa extract; Sparassis crispa extract 3: a) Inoculate Sparassis crispa into PDA liquid medium and culture it at 25 °C in the dark until the OD600 reaches 1.7 to obtain a strain solution; b) Take the strain solution and inoculate it into Sparassis crispa liquid medium 1 at an inoculation amount of 10 - 12 v / v%, and perform shaking culture at 150 rpm and 26 - 30 °C for 15 days to obtain a culture medium; c) Subject the culture medium to ultrasonic disruption treatment with an ultrasonic frequency of 40 kHz and an ultrasonic time of 15 minutes. Then, enzymatically hydrolyze it with cellulase for 2 h, centrifuge to obtain the supernatant. The addition amount of cellulase accounts for 5 wt% of the culture medium, and the cellulase activity concentration is 20,000 U / g; d) Use an ultrafiltration membrane with a molecular weight cut-off range of 0 - 10 kDa to retain the active substances in the supernatant, and perform freeze-drying on the retained fraction to obtain Sparassis crispa extract; Sparassis crispa extract 4: Different from Sparassis crispa extract 1, the Sparassis crispa liquid medium used in b) is Sparassis crispa liquid medium 2; Sparassis crispa extract 5: Different from Sparassis crispa extract 1, the Sparassis crispa liquid medium used in b) is Sparassis crispa liquid medium 3; Sparassis crispa extract ①: Different from Sparassis crispa extract 1, the Sparassis crispa liquid medium used in b) is Sparassis crispa liquid medium ①; Sparassis crispa extract ②: Different from Sparassis crispa extract 1, the Sparassis crispa liquid medium used in b) is Sparassis crispa liquid medium ②; Sparassis crispa extract ③: Different from Sparassis crispa extract 1, the Sparassis crispa liquid medium used in b) is Sparassis crispa liquid medium ③; Sparassis crispa extract ④: Different from Sparassis crispa extract 1, the Sparassis crispa liquid medium used in b) is Sparassis crispa liquid medium ④; Sparassis crispa extract ⑤: Different from Sparassis crispa extract 1, step d) is not performed, and the supernatant of step c) is used as Sparassis crispa extract.

[0025] Preparation of a repair composition suitable for sensitive skin: Composition 1: Sparassis crispa extract 1: 5 wt%, Lactobacillus / soybean milk fermentation product filtrate: 1 wt%, Orange fruit extract: 1 wt%, Hexapeptide-1: 0.2 wt%, and the balance is deionized water.

[0026] Composition 2: Sparassis crispa extract 1: 4 wt%, Lactobacillus / soybean milk fermentation product filtrate: 0.5 wt%, Orange fruit extract: 1.5 wt%, Hexapeptide-1: 0.3 wt%, and the balance is deionized water.

[0027] Composition 3: Sparassis crispa extract 1: 6 wt%, Lactobacillus / soybean milk fermentation product filtrate: 1.5 wt%, Orange fruit extract: 0.5 wt%, Hexapeptide-1: 0.1 wt%, and the balance is deionized water.

[0028] Composition ①: Different from Composition 1, it lacks Sparassis crispa extract, and the mass percentage is supplemented with Lactobacillus / soybean milk fermentation product filtrate, Orange fruit extract, and Hexapeptide-1 in a mass ratio of 1:1:0.2; Composition ②: Different from Composition 1, it lacks Lactobacillus / soybean milk fermentation product filtrate, and the mass percentage is supplemented with Sparassis crispa extract 1, Orange fruit extract, and Hexapeptide-1 in a mass ratio of 5:1:0.2; Composition ③: Different from Composition 1, it lacks Orange fruit extract, and the mass percentage is supplemented with Sparassis crispa extract 1, Lactobacillus / soybean milk fermentation product filtrate, and Hexapeptide-1 in a mass ratio of 5:1:0.2; Composition ④: Different from Composition 1, it lacks Hexapeptide-1, and the mass percentage is supplemented with Sparassis crispa extract 1, Lactobacillus / soybean milk fermentation product filtrate, and Orange fruit extract in a mass ratio of 5:1:1.

[0029] Composition ⑤: Replace the Sparassis crispa extract in Composition 1 with a commercially available Sparassis crispa extract; Composition ⑥: Replace the Sparassis crispa extract in Composition ② with a commercially available Sparassis crispa extract; Composition ⑦: Replace the Sparassis crispa extract in Composition ③ with a commercially available Sparassis crispa extract; Composition ⑧: Replace the Sparassis crispa extract in Composition ④ with a commercially available Sparassis crispa extract; Efficacy evaluation: Test 1: According to T / JSRH 003-2023 "Testing of anti-allergic activity of cosmetic raw materials - Hyaluronidase inhibition method", the hyaluronic acid inhibition rates of Sparassis crispa extracts 1-5, ①-⑥, commercially available Sparassis crispa extracts, and compositions 1-3 and ①-⑧ were detected.

[0030] Preparation of test samples: Sparassis crispa extract: The Sparassis crispa extract was prepared into a 5wt% Sparassis crispa extract mixture with deionized water; Composition: No preparation was carried out.

[0031] Test 2: Detect the inhibitory effects of Sparassis crispa extracts 1-5, ①-⑥, commercially available Sparassis crispa extracts, and compositions 1-3 and ①-⑧ on inflammatory factors; Preparation of test samples: Sparassis crispa extract: The Sparassis crispa extract was prepared into a 5wt% Sparassis crispa extract mixture with deionized water; Composition: No preparation was carried out.

[0032] Experimental procedures: Cell seeding: Dilute mouse mononuclear macrophages with cell culture medium to the seeding density (the confluence reaches 45%-60% 24 h after seeding), seed into a 96-well plate, with a liquid volume of 200 μL per well, and place in a CO 2 incubator and culture for 24 ± 2 h.

[0033] Drug administration: Discard the culture medium in the 96-well plate, and carry out induction and drug administration operations. Add 1 mL of the test substance and a culture medium containing 1 μg / mL LPS to the wells of the test substance (using the test sample to be tested), add a cell culture medium containing LPS to the negative control wells, and add a cell culture medium to the blank control wells, 200 μL per well, and continue to culture in a CO 2 incubator for 24 ± 2 h.

[0034] Collection of cell supernatant: After the incubation culture is completed, collect 200 μL of cell culture supernatant into a 1.5 mL sterile centrifuge tube.

[0035] ELISA detection: Detection was carried out according to the operation instructions of the TNF-α ELISA detection kit.

[0036] The experimental results of Tests 1-2 are presented as mean values, as shown in Table 1.

[0037] Table 1 Sample Hyaluronic acid inhibition rate / % TNF-α inhibition rate / % Sample Hyaluronic acid inhibition rate / % TNF-α inhibition rate / % Sparassis crispa extract 1 66.42 38.72 Composition 1 85.37 52.55 Sparassis crispa extract 2 65.05 37.07 Composition 2 83.91 50.42 Sparassis crispa extract 3 64.83 38.24 Composition 3 85.19 51.87 Sparassis crispa extract 4 65.22 37.66 Composition ① 57.84 33.42 Sparassis crispa extract 5 64.91 37.93 Composition ② 76.27 46.44 Sparassis crispa extract ① 54.15 28.43 Composition ③ 72.63 44.57 Sparassis crispa extract ② 57.88 30.35 Composition ④ 73.55 47.65 Sparassis crispa extract ③ 48.12 22.79 Composition ⑤ 83.42 47.39 Sparassis crispa extract ④ 58.32 30.23 Composition ⑥ 76.25 42.52 Sparassis crispa extract ⑤ 65.16 30.47 Composition ⑦ 72.28 40.67 Commercially available Sparassis crispa extract 60.32 35.71 Composition ⑧ 74.42 43.62 By comparing Sparassis crispa extracts 1-5 with commercially available Sparassis crispa extracts, it can be seen that the Sparassis crispa extract cultivated using the medium defined in the present invention has a significant effect of inhibiting hyaluronidase and TNF-α.

[0038] By comparing Sparassis crispa extract 1 with Sparassis crispa extracts ①-④, it can be seen that birch sap, Portulaca oleracea extract, corn extract, and Centella asiatica powder in the medium defined in the present invention have a certain synergistic effect, which can effectively promote the effect of the cultivated Sparassis crispa extract in inhibiting hyaluronidase and TNF-α.

[0039] By comparing Sparassis crispa extract 1 with Sparassis crispa extract ⑤, it can be seen that the active substances with a molecular weight of 0-10 kDa in the Sparassis crispa extract have a better effect of inhibiting TNF-α.

[0040] By comparing Composition 1 with Compositions ①-④, it can be seen that there is a certain synergistic effect among the Sparassis crispa extract, Lactobacillus / soybean milk fermentation product filtrate, orange fruit extract, and Hexapeptide-1 in the composition of the present invention.

[0041] By comparing Composition 1 with Composition ⑤, it can be seen that the inhibitory effect of Sparassis crispa extract 1 in the composition of the present invention on hyaluronidase is similar to that of the commercially available Sparassis crispa extract, but the inhibition rate of TNF-α is better than that of the commercially available Sparassis crispa extract, which is in line with the foregoing inference.

[0042] By comparing the results of Composition 1 with Compositions ①-④ and the results of Composition ⑤ with Compositions ⑥-⑧ respectively, it can be seen that the Sparassis crispa extract has a synergistic effect with Lactobacillus / soybean milk fermentation product filtrate, orange fruit extract, and Hexapeptide-1, but the Sparassis crispa extract prepared by the preparation method provided in the present invention has a better effect than the commercially available Sparassis crispa extract.

[0043] Safety test Taking the "Technical Specifications for Cosmetic Safety" in 2015 as the reference standard, a safety evaluation was carried out on Compositions 1-3. 15 people aged 16-65 were randomly divided into groups of 5 people each, and skin patch tests were carried out respectively.

[0044] Test method: Put the test substance into the patch tester, with a dosage of 0.020-0.025 g. Cover the patch tester with the test substance on the back or the flexor side of the forearm of the subject with a non-irritating cloth-based tape, and gently press with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester for 30 minutes, observe the skin reaction after the indentation disappears. If the result is negative, observe again at 24 hours and 48 hours after the patch test respectively.

[0045] Evaluation criteria: Grade 0: Negative reaction; Grade 1: Suspected reaction, with only faint erythema; Grade 2: Weak positive reaction, with erythema, infiltration, edema, and may have papules; Grade 3: Strong positive reaction, with erythema, infiltration, edema, may have papules, and the reaction may extend beyond the test area; Grade 4: Extremely strong positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and the reaction extends beyond the test area.

[0046] Test result: The skin reactions of all subjects were negative.

[0047] The above test results indicate that the repair composition suitable for sensitive skin provided by the present invention is mild and non-irritating to the skin and is safe to use.

[0048] The embodiments described above are some embodiments of the present invention, rather than all embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

Claims

1. A hydrangea liquid culture medium, characterized in that: The invention comprises the following components in percentage by weight: 5-15% birch sap, 0.5-3.0% purslane extract, 0.5-1.5% corn extract, 0.3-2.0% hydrolyzed peptone, 1.1-1.8% trehalose, 0.01-0.1% centella asiatica powder, and the balance is deionized water; the pH value of the culture medium is 5.8-6.

5.

2. A method for preparing a hydrangea extract, characterized in that: The following steps are involved: a) inoculating the hydrangea into a PDA liquid culture medium, and culturing at 25-28° C. in a dark environment until the OD600 is 1.5±0.2 to obtain a bacterial culture solution; b) taking the bacterial liquid at an inoculum rate of 10-12 v / v% and inoculating it into the hydrangea liquid culture medium of claim 1, and shaking culturing it at 150-200 rpm and 26-30° C. for 10-15 days; c) subjecting the culture fluid to ultrasonic disruption treatment, with an ultrasonic frequency of 30 kHz and an ultrasonic time of 20 minutes, and then using cellulase for enzymatic hydrolysis for 2 hours, and centrifuging to obtain the supernatant, wherein the amount of cellulase added accounts for 4-5wt% of the culture fluid, and the cellulase activity concentration is 20,000 U / g; d) using an ultrafiltration membrane to retain the active substances in the supernatant with a molecular weight cutoff range of 0-10 kDa, and freeze-drying the retained fraction to obtain a hydrangea extract.

3. The use of the hydrangea extract prepared by the method for preparing the hydrangea extract according to claim 2 in preparing cosmetics with repairing effects, characterized in that: The cosmetic is selected from any one of essence, face cream, eye cream and face mask, and the amount of the hydrangea extract added to the cosmetic is 0.5-10wt%.

4. A repair composition suitable for sensitive skin, characterized in that: Contains the following components by mass percentage: The method for preparing a hydrangea extract according to claim 2 comprises 4-6% hydrangea extract, 0.5-1.5% lactobacillus / soymilk fermentation product filtrate, 0.5-1.5% orange fruit extract, 0.1-0.3% hexapeptide-1, and the remainder is deionized water.

5. The repair composition according to claim 4, characterized in that: Contains the following components by mass percentage: The method for preparing a hydrangea extract according to claim 2 comprises 5-6% hydrangea extract, 1-1.5% lactobacillus / soymilk fermentation product filtrate, 1-1.5% orange fruit extract, 0.2-0.3% hexapeptide-1, and the remainder is deionized water.

6. The repair composition according to claim 5, characterized in that: The invention comprises the following components in percentage by weight: 5% hydrangea extract, 1% lactobacillus / soy milk fermentation product filtrate, 1% citrus fruit extract, 0.2% hexapeptide-1, and the balance is deionized water.

Images