Bacillus licheniformis degradation liquid of northeast walnut oil extraction residues

Abstract

The invention relates to a preparation method of bacillus licheniformis degradation liquid of northeast walnut oil extraction residues. Wild pecans in northeast China are rich in yield, and residues after oil extraction are mostly used as feed. The walnuts are rich in protein and grease. The invention relates to bacillus licheniformis (with a chemical name of bacillus licheniformis), which is one kind of bacillus and is a gram-positive thermophilic bacterium which is common in soil, and the bacillus licheniformis is a bacillus licheniformis with a chemical name of bacillus licheniformis (with a chemical name of bacillus licheniformis). Various active enzymes such as protease, amylase, lipase, pectinase, glucanase and cellulase can be generated in the growth and metabolism process, and the northeast walnut oil extraction residues rich in protein and grease can be degraded. The method comprises the following steps: crushing the northeast walnut oil extraction residues, putting the crushed northeast walnut oil extraction residues into a sealable container, adding sterile water, and inoculating a fermentation seed solution of commercially available bacillus licheniformis diluted by normal saline; and uniformly stirring and mixing, sealing and standing at a proper temperature. The method is beneficial to comprehensive utilization of the northeast walnut oil extraction residues.

Description

Technical Field

[0001] The present invention relates to a method for preparing a degradation solution of Bacillus licheniformis for the residue of Northeast walnut oil extraction. Background Art

[0002] Walnuts are rich in protein, the content of which is higher than that of most nuts, making them an excellent source of protein. The wild walnuts in Changbai Mountain in Northeast China have a rich yield, and the residue after oil extraction is mostly used as feed. Bacillus licheniformis (scientific name: Bacillus licheniformis) is a species of the genus Bacillus and is a common Gram-positive thermophilic bacterium in the soil. During its growth and metabolism, it can produce various active enzymes, such as protease, amylase, lipase, pectinase, glucanase, cellulase, etc., which are beneficial to the degradation of the residue of Northeast walnut oil extraction rich in protein and oil. Therefore, we have proposed a method for preparing a degradation solution of Bacillus licheniformis for the residue of Northeast walnut oil extraction. Summary of the Invention

[0003] In order to improve the efficient utilization of the residue of wild walnuts in Northeast China after oil extraction, the present invention provides a method for degradation using Bacillus licheniformis.

[0004] The specific method is as follows:

[0005] After the residue of Northeast walnut oil extraction is crushed, it is placed in a sealable container, and sterile water is added under sterile operation. A fermentation seed solution of commercially available Bacillus licheniformis diluted with normal saline is inoculated. Stir and mix evenly, and place it sealed at an appropriate temperature. The fermentation conditions are: the ratio of material to water is 2:1 - 1:2, the fermentation temperature is 22 - 37 °C, the fermentation time is 1 - 9 days, the concentration of the fermentation seed solution of commercially available Bacillus licheniformis is 50 - 100 cfu / mL (the effective bacteria content of different preparations is different, so the dosage will also vary. In actual use, please strictly follow the instructions on the product manual), and the inoculation amount is 5 - 20%. Detailed Embodiments

[0006] Example 1

[0007] The preparation method is as follows: After 1 kg of the residue of Northeast walnut oil extraction is crushed, it is placed in a 10 L sealable container, and 1000 mL of sterile water is added under sterile operation, with a material-to-water ratio of 1:1. 50 mL of a fermentation seed solution of commercially available Bacillus licheniformis diluted with normal saline and adjusted to a concentration of 50 cfu / mL is inoculated according to the inoculation amount (5%), stirred and mixed evenly, and sealed. The fermentation temperature is 25 °C, and the fermentation time is 3 days. Shake well to obtain the degradation solution of Bacillus licheniformis prepared from the residue of Northeast walnut oil extraction.

[0008] The present invention is beneficial to the comprehensive utilization of the residue of Northeast walnut oil extraction.

Claims

1. The Bacillus licheniformis degradation liquid of the Northeast walnut oil pressing residue is characterized by: The method is to prepare the oil from the residue of walnut oil pressing in Northeast China.

2. The Bacillus licheniformis degradation liquid of the Northeast walnut oil pressing residue is characterized by: The Northeast walnut oil pressing residue is crushed and placed in a sealable container, and the fermented seed liquid of commercially available Bacillus licheniformis diluted with physiological saline is added, stirred and mixed, and placed in a sealed container at an appropriate temperature.

3. The Bacillus licheniformis degradation liquid of the Northeast walnut oil pressing residue is characterized by: The fermentation conditions are: material-water ratio of 2:1-1:2, fermentation temperature of 22-37 degrees, fermentation time of 1-9 days, fermentation seed liquid concentration of commercially available Bacillus licheniformis is 50-100 cfu / mL, and inoculation amount is 5-20%.