Intelligent control method for cultivation process of tremella fuciformis

Abstract

The present application relates to the growth prediction and regulation field of Auricularia arida, and discloses an intelligent control method for the cultivation process of Auricularia arida, which comprises selecting suitable organic raw materials and configuring them into culture medium, inoculating the strain into the culture medium which has been sterilized, and placing the culture medium in a cultivation environment which can regulate humidity, temperature, carbon dioxide concentration and air flow after inoculation, so that the mycelium can expand and grow in the culture medium under suitable environment. The present application sets early observation points to obtain the coverage area and distribution of the mycelium on the culture medium, so that the mycelium expansion state in the cultivation process can be monitored in real time, and the cultivation efficiency and quality of Auricularia arida can be predicted according to the early expansion and growth of the mycelium. The problem of insufficient mycelium expansion can be found in the early stage, which provides a basis for subsequent remedial measures and reduces the risk of affecting the formation of fruit bodies and yield due to poor mycelium growth in the later stage.

Description

Technical Field

[0001] This invention relates to the field of growth prediction and regulation of wrinkled fungus, specifically to an intelligent control method for the cultivation process of wrinkled fungus. Background Technology

[0002] Wrinkled wood ear fungus is an edible fungus belonging to the wood ear family. It is rich in nutrients such as protein, dietary fiber, various vitamins, and minerals, and is commonly used in cooking, including cold dishes, stir-fries, and soups. Furthermore, with the development of modern agricultural technology in recent years, the cultivation of wrinkled wood ear fungus has gradually become more large-scale and intelligent. Through sensor monitoring, environmental control, and data analysis, key growth factors such as temperature, humidity, and carbon dioxide concentration are precisely regulated, providing a stable and ideal cultivation environment for wrinkled wood ear fungus, thereby improving yield and quality.

[0003] In the cultivation of wrinkled wood ear fungus, specially formulated culture media are used. These media typically include organic materials such as sawdust, rice bran, cottonseed hulls, and corn cobs. Through high-temperature sterilization and the addition of appropriate amounts of water and nutrients, a pure and nutrient-rich growth environment is provided for the wrinkled wood ear fungus. During the cultivation of wrinkled wood ear fungus, the mycelium is extremely sensitive to the external environment. Therefore, by observing and analyzing the growth status of the mycelium during the cultivation process, the growth status of the wrinkled wood ear fungus can be predicted, and the cultivation management methods can be adjusted in a timely manner. Summary of the Invention

[0004] To address the problems existing in the prior art, the present invention aims to provide an intelligent control method for the cultivation process of wrinkled fungus, which can predict the suitability of the growth conditions of wrinkled fungus by observing the mycelial growth on the culture medium during the cultivation process, and reasonably regulate the cultivation conditions during the cultivation process.

[0005] To achieve the above objectives, the present invention provides the following technical solution: an intelligent control method for the cultivation process of wrinkled black fungus, the method comprising the following steps:

[0006] Select suitable organic raw materials and prepare them into a culture medium. Then, inoculate the inoculum into the sterilized culture medium. After inoculation, place the culture medium in a cultivation environment with adjustable humidity, temperature, carbon dioxide concentration and air circulation so that the mycelium can expand and grow in the culture medium under suitable conditions.

[0007] The first day of inoculation in the culture medium is set as the cultivation start date. The induction period is set according to the cultivation history data. An early observation point is set between the cultivation start date and the induction period. When the cultivation time reaches the early observation point, the culture medium image is obtained and the coverage area and density distribution of mycelia on the culture medium are obtained.

[0008] Set a coverage area threshold and compare the coverage area of ​​mycelium on the culture medium with the coverage area threshold. When the coverage area of ​​mycelium on the culture medium is less than the coverage area threshold, it is estimated that the mycelium will lead to a decrease in the yield and quality of wrinkled wood ear fungus under this growth trend, and a production environment control strategy is implemented.

[0009] The production environment control strategy includes determining the growth tendency of mycelia based on the growth density of mycelia in different directions on the culture medium, dividing the area into high-tendency and low-tendency areas, and implementing remedial measures for areas with low mycelial growth tendency.

[0010] The specific method for determining mycelial growth tendency is to divide the culture medium image into a 3×3 matrix region, namely the middle region, upper region, lower region, left region, right region, upper left region, upper right region, lower left region, and lower right region. A coverage threshold is set, and the area ratio occupied by mycelia in each region is obtained. If the area ratio occupied by mycelia in the corresponding region exceeds the coverage threshold, the region is marked as a high-tendency region; if the area ratio occupied by mycelia in the corresponding region does not exceed the coverage threshold, the region is marked as a low-tendency region.

[0011] Set up observation points for the remedial effect, obtain the ratio of the difference between high and low tendency areas at the early observation points, and obtain the ratio of the difference between high and low tendency areas at the remedial effect observation points. The specific method for obtaining the ratio of the difference between high and low tendency areas is: average the area ratio of the mycelium occupying the corresponding area in the high tendency area to obtain the average high tendency ratio, average the area ratio of the mycelium occupying the corresponding area in the low tendency area to obtain the average low tendency ratio, and divide the average low tendency ratio by the average high tendency ratio to obtain the ratio of the difference between high and low tendency areas.

[0012] The difference between the high and low tendency areas obtained from the early observation point and the remedial effect observation point is used to obtain the remedial ratio difference value. A ratio difference threshold is set, and the remedial ratio difference value is compared with the ratio difference threshold. When the remedial ratio difference value is greater than the ratio difference threshold, a notification of successful adjustment of the low tendency area is generated. When the remedial ratio difference value is less than or equal to the ratio difference threshold, the formation period extension prediction strategy is implemented to obtain the predicted growth cycle. The formation period extension prediction strategy includes obtaining the growth amount of the low tendency area by the difference between the average low tendency ratio at the remedial effect observation point and the average low tendency ratio at the early observation point, dividing the growth amount of the low tendency area by the number of days from the early observation point to the remedial effect observation point to obtain the predicted daily growth amount of the low tendency area, obtaining the mycelial proportion difference Dc=k-Db from the average low tendency ratio Db at the remedial effect observation point, where k is an adjustment factor, dividing the mycelial proportion difference by the predicted daily growth amount of the low tendency area to obtain the predicted growth cycle, and summing the predicted growth cycle with the number of days already cultivated to replace the induction formation period, so as to extend the cultivation cycle of wrinkled fungus.

[0013] In some implementations, the remedy for low-prone areas is to prepare an appropriate amount of nutrient solution in a sterile environment, apply it evenly to the low-prone area using a pipette or sprayer, promote the improvement of the nutrient level in the area, and enhance the airflow circulation in the cultivation environment.

[0014] In some embodiments, the culture medium is a square culture medium so that when the image acquisition device acquires image information of mycelia on the culture medium, the culture medium can cover the entire acquired image.

[0015] The present invention further provides a computer-readable storage medium storing a computer program, which is executed by a processor to implement the above-described intelligent control method for the cultivation process of *Auricularia auricula-judae*.

[0016] The technical solution provided by this invention has the following advantages compared with the prior art:

[0017] Firstly, this invention sets early observation points to obtain the coverage area and distribution of mycelium on the culture medium, enabling real-time monitoring of mycelial expansion during cultivation. Based on the early expansion and growth of mycelium, the cultivation efficiency and quality of wrinkled wood ear mushrooms can be estimated. Problems of insufficient mycelial expansion can be detected early, providing a basis for subsequent remedial measures and reducing the risk of poor mycelial growth affecting fruiting body formation and yield in the later stages.

[0018] Secondly, by dividing the culture medium into multiple regions, this invention can accurately quantify the mycelial coverage of each region, reflect the differences in mycelial distribution in the culture medium in real time, and provide targeted remediation for low-tendency areas based on the differences in mycelial growth in various directions, ensuring the balanced expansion of mycelium in each region, thereby achieving higher consistency during the induction formation period.

[0019] Thirdly, this invention transforms the difference in mycelial coverage between low-tendency and high-tendency areas before and after remediation into numerical indicators, which can provide timely feedback on the remediation effect and facilitate cultivators to quickly adjust management strategies or take further measures. When the remediation effect is not ideal, the induction period can be extended by estimating the efficiency of mycelial expansion and growth in low-tendency areas, allowing low-tendency areas to have more growth time, thereby achieving overall balanced mycelial growth. Attached Figure Description

[0020] Figure 1 This is a flowchart illustrating an intelligent control method for the cultivation process of wrinkled fungus according to the present invention.

[0021] Figure 2 This is a schematic diagram of the image region division of the culture medium according to the present invention. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] It is understood that the term "a" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element can be one, while in another embodiment, the number of the element can be multiple, and the term "a" should not be understood as a limitation on the number.

[0024] This invention provides an intelligent control method for the cultivation process of wrinkled fungus, such as... Figure 1 and Figure 2 As shown, the method includes the following steps:

[0025] Step 1: Select suitable organic raw materials and prepare them into a culture medium. Sterilize the culture medium at high temperature to eliminate miscellaneous bacteria and ensure a pure nutrient environment. Then, inoculate the inoculum into the sterilized culture medium. After inoculation, place the culture medium in a cultivation environment with adjustable humidity, temperature, carbon dioxide concentration and air circulation so that the mycelium can expand and grow in the culture medium in a suitable environment, absorb nutrients and decompose organic matter.

[0026] Step two: The first day of inoculation in the culture medium is set as the cultivation start date. Based on the historical cultivation data of wrinkled wood ear fungus, the induction period is set. The induction period refers to the period after the mycelium has fully filled the culture medium. By adjusting environmental conditions (such as reducing carbon dioxide concentration, appropriately increasing ventilation, and adjusting temperature and humidity), the mycelium is induced to transform into fruiting bodies, i.e., wrinkled wood ear fungus. The specific time for setting the induction period should be based on the average time taken from the start of cultivation to the induction of fruiting bodies in the historical cultivation data of wrinkled wood ear fungus. In practice, it is generally 30-40 days after inoculation. An early observation point was set between the start of cultivation and the induction period. When the cultivation time reached the early observation point, image information of the culture medium was acquired through image acquisition equipment, and the mycelial contour in the image was extracted using image segmentation algorithms (such as threshold segmentation, Canny edge detection, etc.) to obtain the coverage area and density distribution of mycelia on the culture medium. It should be noted that the setting of the early observation point should be combined with the historical cultivation data of Auricularia auricula-judae. Generally, the expansion rate and coverage area of ​​mycelia on the culture medium will show significant changes 10-15 days after inoculation. Therefore, the early observation point should be set 10-15 days after the start of cultivation.

[0027] Step 3: Set a coverage area threshold. Compare the coverage area of ​​the mycelium on the culture medium with the coverage area threshold. When the coverage area of ​​the mycelium on the culture medium is greater than or equal to the coverage area threshold, it indicates that the mycelium is growing well on the culture medium when it reaches the early observation point, and a notification of good cultivation status can be generated. When the coverage area of ​​the mycelium on the culture medium is less than the coverage area threshold, it indicates that the mycelium is not growing as expected on the culture medium when it reaches the early observation point. The slower growth rate results in a smaller initial coverage area. Therefore, it can be predicted that the mycelium will lead to a decrease in the yield and quality of wrinkled wood ear fungus under this growth trend, and a dynamic remedial strategy for cultivation should be implemented.

[0028] Step four, the cultivation dynamic remedial strategy includes determining the growth tendency of mycelium based on the growth density of mycelium in different directions on the culture medium, and implementing remedial measures for areas with lower mycelial growth tendency.

[0029] The specific method for determining mycelial growth tendency is to divide the culture medium image into a 3×3 matrix region, namely the middle region, upper region, lower region, left region, right region, upper left region, upper right region, lower left region, and lower right region (e.g., Figure 2A coverage threshold is set, and the area ratio of mycelium in each region is obtained. This ratio is compared to the coverage threshold. If the mycelium's area ratio exceeds the threshold, the region is considered to have a high growth tendency and is marked as a high-tendency region. Conversely, if the ratio is below the threshold, the region is considered to have a low growth tendency and is marked as a low-tendency region. For example, if the early observation point is set to 10 days, an image of the culture medium is acquired on the 10th day of cultivation. The mycelium coverage area is compared to a coverage threshold of 30%, and the mycelium coverage area is set to 25%. It's important to note that the mycelium coverage area is determined by the proportion of the mycelium-covered area to the total image area of ​​the culture medium. For instance, if the culture medium image has 10,000 pixels and mycelium occupies 2,500 pixels, the mycelium coverage area is 25%. Since the mycelium coverage area is less than the threshold, a dynamic remedial cultivation strategy is implemented. The coverage threshold can be set based on the historical average of different regions in historical cultivation data, or it can be set based on the actual detected mycelial coverage area. Since the mycelial coverage area is 25%, the coverage threshold is also set to 25%. The area ratios of mycelium occupying the middle, upper, lower, left, right, upper left, upper right, lower left, and lower right regions are set to 40%, 35%, 35%, 5%, 28%, 15%, 26%, 15%, and 26%, respectively. From the above, it can be concluded that the left region (5%), upper left region (15%), and lower left region (15%) are low-tendency regions, and the remaining regions are high-tendency regions.

[0030] The remedial measures for low-prone areas are as follows: Prepare an appropriate amount of nutrient solution under sterile conditions and apply it evenly to the low-prone areas using a pipette or sprayer to increase the nutrient level in these areas, bringing them closer to the mycelial growth conditions of high-prone areas. This is because the culture environment of the *Auricularia auricula-judae* established in this scheme is appropriately set, avoiding abnormal mycelial growth trends caused by environmental factors. If the mycelial growth trend is still unsatisfactory, after ruling out environmental factors, it is basically due to uneven mixing of nutrients and other additives in the culture medium during preparation, resulting in low local nutrient concentrations and affecting the mycelial expansion rate. Simultaneously, appropriately enhancing airflow circulation in the cultivation environment helps create a more uniform microenvironment, improving gas exchange and temperature and humidity balance after nutrient remediation in localized areas.

[0031] After implementing remedial measures, an observation point for the remedial effect is set. This observation point can be set 5-7 days after the early observation point. First, the difference ratio between high and low tendency areas is obtained at the early observation point, and then the difference ratio is obtained again at the remedial effect observation point. The difference between the two high and low tendency area difference ratios obtained at the early observation point and the remedial effect observation point is used to obtain the remedial ratio difference value. A ratio difference threshold is set, and the remedial ratio difference value is compared with the ratio difference threshold. A corresponding response is taken based on the comparison result. Specifically, when the remedial ratio difference value is greater than the ratio difference threshold, it means that after implementing remedial measures for the low tendency area, the mycelial growth efficiency in the low tendency area has caught up with the growth efficiency in the high tendency area, and the remedial measures have achieved the desired effect. In this case, a notification of successful adjustment of the low tendency area is generated. When the remedial ratio difference value is less than or equal to the ratio difference threshold, it means that after implementing remedial measures for the low tendency area, the mycelial growth efficiency in the low tendency area still has not caught up with the growth efficiency in the high tendency area, and the effect of the remedial measures is not ideal. In this case, a formation period extension prediction strategy is implemented.

[0032] The specific method for obtaining the difference ratio between high and low tendency regions is as follows: Average the area occupied by mycelia in the high tendency region to obtain the average high tendency ratio; average the area occupied by mycelia in the low tendency region to obtain the average low tendency ratio; then, divide the average low tendency ratio by the average high tendency ratio to obtain the difference ratio between the high and low tendency regions. It should be noted that when obtaining the average high and low tendency ratios at the remedial effect observation point, if the average low tendency ratio is greater than or equal to the average high tendency ratio, it is directly determined that the mycelial growth efficiency in the low tendency region has caught up with the growth efficiency in the high tendency region, the remedial measures have achieved the desired effect, and a notification of successful adjustment of the low tendency region is generated. Taking the above embodiments as an example, at the early observation point, the area proportions of mycelia occupying the middle, upper, lower, left, right, upper left, upper right, lower left, and lower right regions are set to 40%, 35%, 35%, 5%, 28%, 15%, 26%, 15%, and 26%, respectively. This yields an average high-tendency proportion of 31.6% and an average low-tendency proportion of 11.6%, meaning the difference between high and low-tendency regions at the early observation point is 36%. After remedial measures are implemented, at the remedial effect observation point, the area proportions of mycelia occupying the middle, upper, lower, left, right, and upper left regions are set to... The area ratios of the top right, bottom left, and bottom right regions are 45%, 40%, 40%, 30%, 35%, 30%, 40%, 28%, and 41%, respectively. The average low-preference ratio of the left region (30%), top left region (30%), and bottom left region (28%) in the low-preference region is 29.3%, while the average high-preference ratio is 40.1%. Therefore, the difference between the high and low-preference regions at the observation point of the remediation effect is 73%, and the remediation ratio difference is 37%. The ratio difference threshold is set at 25%. Since the remediation ratio difference is greater than the ratio difference threshold, a notification of successful adjustment of the low-preference region is generated.

[0033] The strategy for extending the formation period involves calculating the growth rate of the low-tendency area by subtracting the average low-tendency ratio at the remedial effect observation point from the average low-tendency ratio at the early observation point. The estimated daily growth rate of the low-tendency area is then obtained by dividing the growth rate of the low-tendency area by the number of days from the early observation point to the remedial effect observation point. The mycelial proportion difference (Dc) is calculated using the average low-tendency ratio (Db) at the remedial effect observation point (k is a regulating factor of 0.7-1). The estimated growth cycle is then calculated by dividing the mycelial proportion difference by the estimated daily growth rate of the low-tendency area. This estimated growth cycle is then summed with the number of days already cultivated to replace the induction formation period, thereby extending the cultivation cycle of *Auricularia auricula-judae*. For example, a preset induction formation period can be set. The period is 30 days. When the difference in the remediation ratio is less than the threshold, the average low tendency ratio at the remediation effect observation point is 21.6%, and the average low tendency ratio at the early observation point is 11.6%. This indicates that the growth of the low tendency area is 10%. If the number of days from the early observation point to the remediation effect observation point is set to 5 days, then the estimated daily growth of the low tendency area is 2%. If the adjustment factor k is set to 0.7, the difference in mycelial proportion is 48.4%, which means the estimated growth cycle is 24 days. Since the number of days already cultivated is 15 days, the sum of the estimated growth cycle and the number of days already cultivated is 39 days. Therefore, the induction period is set from 30 days to 39 days to extend the cultivation cycle of the wrinkled fungus. This is because if the mycelial growth trend remains unsatisfactory after remedial measures are implemented, the abnormal mycelial growth trend may be due to insufficient mixing or uniform spraying during sowing or liquid spawn distribution. Insufficient inoculation in certain areas directly leads to low initial mycelial density. Therefore, when remedial measures are ineffective, extending the induction period allows mycelia in low-prone areas more time for compensatory growth, thereby narrowing the growth gap between high and low-prone areas and achieving overall balanced mycelial growth. Although this extends the cultivation cycle, it ensures the quality of the wrinkled fungus, guaranteeing excellent growth and consistent quality. The above method, centered on data quantification, achieves scientific determination of growth differences through precise monitoring of mycelial coverage in various regions. After remedial measures, if the mycelial growth in the low-prone areas significantly improves, narrowing the gap with the dominant areas and making the overall expansion more balanced, a notification will be issued in a timely manner. Conversely, if the growth is not improved, the induction period will be extended. Its advantages lie in real-time feedback, risk warning, and dynamic control. At the same time, extending the induction period provides sufficient compensation opportunities for weak growth areas. This method can reduce the risk of abnormal mycelial growth trends during the growth process, effectively improve the balance of mycelium during the cultivation of wrinkled wood ear fungus and the consistency of fruiting body formation in the later stage, so that the cultivation benefits of wrinkled wood ear fungus can be guaranteed.

[0034] It should be noted that the culture medium used in this scheme should be made into a square shape. Currently, many cultivation systems design the shape of the culture medium according to production needs and equipment conditions. The use of square culture medium in this scheme has the following advantages: First, it facilitates standardized production. Square culture medium is easy to arrange, stack and manage uniformly. Under the same area, the square design is usually easier to make full use of the cultivation space. Second, when the image information of mycelium on the culture medium is obtained by the image acquisition device, the square culture medium can cover the entire acquired image, so that the growth of wrinkled wood ear fungus can be more reasonably estimated based on the area covered by mycelium.

[0035] In summary, this invention aims to design an intelligent control method for the cultivation process of *Auricularia auricula-judae* (wrinkled fungus). To predict the suitability of growth conditions and implement reasonable regulation, this invention establishes early observation points to obtain the coverage area and distribution of mycelium on the culture medium. This allows for real-time monitoring of mycelial expansion during cultivation, and the cultivation efficiency and quality of *Auricularia auricula-judae* can be predicted based on early mycelial growth. It can identify insufficient mycelial expansion early on, providing a basis for subsequent remedial measures and reducing the risk of poor mycelial growth affecting fruiting body formation and yield later. By dividing the culture medium into multiple regions, the mycelial coverage in each region can be accurately quantified, reflecting the differences in mycelial distribution across regions in real time. Based on the differences in mycelial growth in different directions, targeted remedial measures can be taken for low-prone areas, ensuring balanced mycelial expansion in all regions and achieving higher consistency during the induction formation period. By converting the difference in mycelial coverage between low-prone and high-prone areas before and after remediation into numerical indicators, the effectiveness of remediation can be promptly reported. This allows growers to quickly adjust management strategies or take further measures. When the remediation effect is unsatisfactory, the induction period can be extended by predicting the efficiency of mycelial expansion in low-prone areas, allowing these areas more time to grow and thus achieving balanced overall mycelial growth. This ensures the quality of subsequent fruiting bodies (wrinkled fungus). Overall, by acquiring mycelial growth data in the early stages and after remediation, implementing predictive feedback, and intervening promptly, it helps reduce the adverse effects of localized weak growth on the final yield and quality of wrinkled fungus, ensuring an ideal cultivation process.

[0036] The processes described above with reference to the flowcharts in the embodiments disclosed in this invention can be implemented as computer software programs. Embodiments of this invention include a computer program product comprising a computer program carried on a computer-readable medium, the computer program containing program code for performing the methods shown in the flowcharts. In such embodiments, the computer program can be downloaded and installed from a network via a communication component, and / or installed from a removable medium. When the computer program is executed by a central processing unit, it performs the functions defined in the methods of this application. It should be noted that the computer-readable medium described above in this application can be a computer-readable signal medium or a computer-readable storage medium, or any combination of the two. A computer-readable storage medium can be, for example, but not limited to, an electrical, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any combination thereof. More specific examples of computer-readable storage media may include, but are not limited to: an electrical connection having one or more conductor segments, a portable computer disk, a hard disk, a random access memory, a read-only memory, an erasable programmable read-only memory, an optical fiber, a portable compact disk read-only memory, an optical storage device, a magnetic storage device, or any suitable combination thereof. In this application, a computer-readable storage medium can be any tangible medium containing or storing a program that can be used by or in connection with an instruction execution system, apparatus, or device. In this application, a computer-readable signal medium can include a data signal propagated in baseband or as part of a carrier wave, carrying computer-readable program code. Such propagated data signals can take various forms, including but not limited to electromagnetic signals, optical signals, or any suitable combination thereof. A computer-readable signal medium can also be any computer-readable medium other than a computer-readable storage medium, which can send, propagate, or transmit a program for use by or in connection with an instruction execution system, apparatus, or device. The program code contained on the computer-readable medium can be transmitted using any suitable medium, including but not limited to: wireless segments, wire segments, optical cables, RF, etc., or any suitable combination thereof.

[0037] The flowcharts and block diagrams in the accompanying drawings illustrate the architecture, functionality, and operation of possible implementations of systems, methods, and computer program products according to various embodiments of the present invention. In this regard, each block in a flowchart or block diagram may represent a module, segment, or portion of code containing one or more executable instructions for implementing a specified logical function. It should also be noted that in some alternative implementations, the functions indicated in the blocks may occur in a different order than those indicated in the drawings. For example, two consecutively indicated blocks may actually be executed substantially in parallel, and they may sometimes be executed in reverse order, depending on the functions involved. It should also be noted that each block in the block diagrams and / or flowcharts, and combinations of blocks in the block diagrams and / or flowcharts, can be implemented using a dedicated hardware-based system that performs the specified function or operation, or using a combination of dedicated hardware and computer instructions.

[0038] Those skilled in the art should understand that the above description is only a specific embodiment of this application, but the protection scope of this application is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the protection scope of this application.

Claims

1. A method for intelligent control of the cultivation process of wrinkled black fungus, characterized in that, The method includes the following steps: Select suitable organic raw materials and prepare them into a culture medium. Then, inoculate the inoculum into the sterilized culture medium. After inoculation, place the culture medium in a cultivation environment with adjustable humidity, temperature, carbon dioxide concentration and air circulation so that the mycelium can expand and grow in the culture medium under suitable conditions. The first day of inoculation in the culture medium is set as the cultivation start date. The induction period is set according to the cultivation history data. An early observation point is set between the cultivation start date and the induction period. When the cultivation time reaches the early observation point, the culture medium image is obtained and the coverage area and density distribution of mycelia on the culture medium are obtained. Set a coverage area threshold and compare the coverage area of ​​mycelium on the culture medium with the coverage area threshold. When the coverage area of ​​mycelium on the culture medium is less than the coverage area threshold, it is estimated that the mycelium will lead to a decrease in the yield and quality of wrinkled wood ear fungus under this growth trend, and a production environment control strategy is implemented. The production environment control strategy includes determining the growth tendency of mycelia based on the growth density of mycelia in different directions on the culture medium, dividing the area into high-tendency and low-tendency areas, and implementing remedial measures for areas with low mycelial growth tendency. The specific method for determining mycelial growth tendency is to divide the culture medium image into a 3×3 matrix region, namely the middle region, upper region, lower region, left region, right region, upper left region, upper right region, lower left region, and lower right region. A coverage threshold is set, and the area ratio occupied by mycelia in each region is obtained. If the area ratio occupied by mycelia in the corresponding region exceeds the coverage threshold, the region is marked as a high-tendency region; if the area ratio occupied by mycelia in the corresponding region does not exceed the coverage threshold, the region is marked as a low-tendency region. Set up observation points for the remedial effect, obtain the ratio of the difference between high and low tendency areas at the early observation points, and obtain the ratio of the difference between high and low tendency areas at the remedial effect observation points. The specific method for obtaining the ratio of the difference between high and low tendency areas is: average the area ratio of the mycelium occupying the corresponding area in the high tendency area to obtain the average high tendency ratio, average the area ratio of the mycelium occupying the corresponding area in the low tendency area to obtain the average low tendency ratio, and divide the average low tendency ratio by the average high tendency ratio to obtain the ratio of the difference between high and low tendency areas. The difference between the high and low tendency areas obtained from the early observation point and the remedial effect observation point is used to obtain the remedial ratio difference value. A ratio difference threshold is set, and the remedial ratio difference value is compared with the ratio difference threshold. When the remedial ratio difference value is greater than the ratio difference threshold, a notification of successful adjustment of the low tendency area is generated. When the remedial ratio difference value is less than or equal to the ratio difference threshold, the formation period extension prediction strategy is implemented to obtain the predicted growth cycle. The formation period extension prediction strategy includes obtaining the growth amount of the low tendency area by the difference between the average low tendency ratio at the remedial effect observation point and the average low tendency ratio at the early observation point, dividing the growth amount of the low tendency area by the number of days from the early observation point to the remedial effect observation point to obtain the predicted daily growth amount of the low tendency area, obtaining the mycelial proportion difference Dc=k-Db from the average low tendency ratio Db at the remedial effect observation point, where k is an adjustment factor, dividing the mycelial proportion difference by the predicted daily growth amount of the low tendency area to obtain the predicted growth cycle, and summing the predicted growth cycle with the number of days already cultivated to replace the induction formation period, so as to extend the cultivation cycle of wrinkled fungus.

2. The intelligent control method for the cultivation process of wrinkled black fungus according to claim 1, characterized in that, The remedial measures for low-prone areas are as follows: prepare an appropriate amount of nutrient solution in a sterile environment, apply it evenly to the low-prone area using a pipette or sprayer to promote the improvement of the nutrient level in the area, and enhance the air circulation in the cultivation environment.

3. The intelligent control method for the cultivation process of wrinkled fungus according to claim 2, characterized in that, The culture medium is a square culture medium so that when the image acquisition device acquires image information of mycelia on the culture medium, the culture medium can cover the entire acquired image.

4. A computer-readable storage medium, characterized in that, The computer-readable storage medium stores a computer program, which is executed by a processor to implement the intelligent control method for the cultivation process of wrinkled fungus as described in any one of claims 1-3.

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