Application of American cockroach powder in medicine for treating pulmonary sarcoidosis

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and provides application of American cockroach powder in a medicine for treating pulmonary sarcoidosis. The invention provides a new application of periplaneta americana in medicine, the number of nodules of a pulmonary nodule mouse model can be remarkably reduced, and a new direction is provided for development of the traditional Chinese medicine periplaneta americana.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and specifically relates to the application of Periplaneta americana in the treatment of pulmonary nodules. Background Art

[0002] Disclosing the information of this background art is intended to increase the understanding of the overall background of the present invention, and it is not necessarily regarded as an admission or an implication in any form that this information constitutes the prior art already known to those of ordinary skill in the art.

[0003] Sarcoidosis is a granulomatous inflammatory disease of unknown etiology that involves multiple systems and organs, and the respiratory system is the most common, accounting for about 90% of all sarcoidosis; about 20% of patients will develop pulmonary fibrosis. Therefore, the treatment of pulmonary nodules is very necessary.

[0004] The etiology of pulmonary sarcoidosis has not been clearly defined, and it is generally believed to be closely related to genetic factors and environmental factors. The occurrence of granulomas involves various immune responses and multiple cells. T lymphocytes and macrophages are the key cells in the inflammatory response of sarcoidosis. Dendritic cells migrate antigens to local mediastinal lymph nodes and become antigen-presenting cells, which then activate T lymphocytes. Macrophages also have the ability to present antigens. After being activated by antigens, they differentiate into epithelioid cells, surrounded by lymphocytes, plasma cells and fibroblasts, forming the characteristic granulomas of sarcoidosis.

[0005] Modern medicine has no suitable drug treatment for most asymptomatic patients. When the disease progresses and lung function declines, treatment is required to protect lung function and improve quality of life. Commonly used treatment drugs include glucocorticoids (GC), cytotoxic drugs, and biological agents. It can be seen that modern medicine has no effective treatment for common ordinary pulmonary nodules. When it progresses and deteriorates, it is already too late to treat.

[0006] Traditional Chinese medicine believes that the etiology of pulmonary sarcoidosis is the deficiency of healthy qi and the intermingling of phlegm and stasis. Therefore, the treatment should focus on strengthening the spleen and tonifying the lung, clearing heat and resolving phlegm, and promoting blood circulation to remove stasis. Periplaneta americana is the dried body of the insect Periplaneta americana American cockroach (Linnaeus.). It has the functions of promoting blood circulation to remove stasis, clearing heat and detoxifying, detumescence and promoting granulation, and reducing accumulation, and is used for mass accumulation. Summary of the Invention

[0007] In view of the problems in the current treatment of pulmonary nodules, the present invention provides an application of Periplaneta americana powder in drugs for treating pulmonary sarcoidosis.

[0008] To achieve the above object, the present invention adopts the following technical solutions.

[0009] Application of Periplaneta americana powder in drugs for treating pulmonary sarcoidosis. The Periplaneta americana powder is Periplaneta americana American cockroachThe fine powder obtained by sterilizing, drying and pulverizing (Linnaeus.).

[0010] A traditional Chinese medicine composition for treating lung nodules, comprising the following raw materials in parts by weight: Periplaneta americana 3 - 9 parts, Astragalus membranaceus 3 - 10 parts, Scutellaria baicalensis 3 - 10 parts, Fritillaria thunbergii 5 - 10 parts, Prunella vulgaris 10 - 15 parts, Pinellia ternata 3 - 9 parts, Paeonia lactiflora 5 - 10 parts, Hedyotis diffusa 6 - 9 parts. The above formula has the effects of soothing the liver and regulating qi, softening hardness and dissipating nodules.

[0011] Preferably, the above traditional Chinese medicine composition comprises the following raw materials in parts by weight: Periplaneta americana 3 parts, Astragalus membranaceus 3 parts, Cyperus rotundus 3 parts, Pinellia ternata 2 parts, Prunella vulgaris 4 parts, Fritillaria thunbergii 2 parts, Paeonia lactiflora 2 parts, Hedyotis diffusa 2 parts.

[0012] The traditional Chinese medicine composition is obtained by compatibility of Periplaneta americana with other traditional Chinese medicines. The other traditional Chinese medicines can be directly pulverized powders or powders prepared after extraction, concentration and drying. The extraction solvent is preferably water.

[0013] A drug prepared from the above traditional Chinese medicine composition. The drug also includes pharmaceutically acceptable excipients. The drug can be in dosage forms such as powder, water pill, granule, tablet, capsule, etc.

[0014] The present invention has the following advantages: The present invention provides a new use of Periplaneta americana powder in medicine, which can significantly reduce the number of nodules in a mouse model of lung nodules, providing a new direction for the development of traditional Chinese medicine Periplaneta americana. Description of the Drawings

[0015] Figure 1 is the relative mRNA expression level of TNF-α in mice of different treatment groups; Figure 2 is the relative mRNA expression level of IL-1β in mice of different treatment groups; Figure 3 is the relative mRNA expression level of IL-6 in mice of different treatment groups; Figure 4 is the relative mRNA expression level of IL-8 in mice of different treatment groups; Among them, * indicates P < 0.05 compared with the blank group, ※ indicates P < 0.01 compared with the blank group, indicates P < 0.05 compared with the model group. Detailed Embodiments

[0016] The present invention will be further described below in conjunction with embodiments and drawings, but the present invention is not limited by the following embodiments.

[0017] Example 1 Preparation of Capsules After Periplaneta americana is sterilized by moist heat and dried, it is crushed into fine powder (passing through 80-mesh sieve), dry-pressed into granules (without adding excipients), filled into capsules, and each capsule contains 0.5 g of Periplaneta americana powder.

[0018] Example 2 Preparation of Compound Pills After Periplaneta americana is sterilized by moist heat and dried, it is crushed into fine powder (passing through 80-mesh sieve) to obtain Periplaneta americana powder; Take 180 g of Astragalus membranaceus, 180 g of Cyperus rotundus, 120 g of Pinellia ternata processed with ginger, 240 g of Prunella vulgaris, 120 g of Fritillaria thunbergii, 120 g of Paeonia lactiflora, 120 g of Hedyotis diffusa, crush them into fine powder, mix evenly with 180 g of Periplaneta americana powder, make into pills, and dry to obtain. The specification of the pills is that every 10 pills weigh 1 g, and the packaging is 6 g per bag.

[0019] Example 3 Preparation of Compound Capsules After Periplaneta americana is sterilized by moist heat and dried, it is crushed into fine powder (passing through 80-mesh sieve) to obtain Periplaneta americana powder; Take 180 g of Astragalus membranaceus, 180 g of Cyperus rotundus, 120 g of Pinellia ternata processed with ginger, 240 g of Prunella vulgaris, 120 g of Fritillaria thunbergii, 120 g of Paeonia lactiflora, 120 g of Hedyotis diffusa, decoct twice with water. Add 10 times the amount of water for the first time and 8 times the amount of water for the second time, decoct for 2 hours each time, filter, combine the decoction liquids, and concentrate the decoction liquid under reduced pressure to a clear paste with a relative density of 1.32 (60 °C); Add 180 g of Periplaneta americana powder to the clear paste, mix evenly, dry under reduced pressure, crush into fine powder (passing through 80-mesh sieve), and fill into capsules to obtain. Each capsule contains 0.5 g, and the amount equivalent to the original medicinal materials is 1.5 g.

[0020] Application Example 1 Therapeutic Effect of Periplaneta americana on Mice with Lung Nodules 1. Experimental Animals 57BL / 6 male mice, weighing 16 - 18 g, are raised under SPF conditions.

[0021] 2. Drugs Different preparations obtained in Example 1, Example 2, and Example 3 of the present invention.

[0022] 3. Dosage Calculated according to the human dosage, assuming the human weight is calculated as 70 kg, the human dosage is 100 mg / kg, and the mouse dosage is 910 mg / kg.

[0023] 4. Experimental Method Establishing a mouse model of pulmonary nodules using Propionibacterium acnes: Fifty male C57BL / 6 mice were conventionally adaptively fed for 7 days and divided into a blank group, a model group, an Example 1 group, an Example 2 group, and an Example 3 group, with 10 mice in each group. On the first day of modeling, 0.25 mL of a Propionibacterium acnes suspension with a concentration of 2 mg / mL was intraperitoneally injected. Starting from the 7th day, a Propionibacterium acnes suspension with a concentration of 10 mg / mL was used for nasal and oral instillation, once every 3 days, 0.05 mL / mouse / time, and gavage administration was started. The blank group and the model group were gavaged with an equal volume of normal saline once a day for 30 consecutive days. Subsequently, during the experiment, the behavior and appearance of the mice were closely observed, and the mice were fasted 24 hours before the end of the experiment, but not deprived of water.

[0024] After sacrificing the mice by cervical dislocation, the whole lungs were quickly removed, rinsed with pre-cooled normal saline, and the water was blotted dry with filter paper. The apparent state of the lung tissues of mice in each group was observed.

[0025] The lungs were ground in liquid nitrogen and stored at -80 °C for later use. The internal reference gene was β-actin, and the primers for the target genes TNF-α, IL-1β, IL-6, and IL-8 were synthesized by Jilin Kumei Biotechnology Co., Ltd., and the working solution concentration was configured to be 10 μmoL / L. After the experiment, the amplification curves and melting curves were verified, the experimental data were exported, the Ct values of each target gene were analyzed, and then the 2 (△△Ct) method was used to calculate the relative expression levels of each target gene mRNA through the internal reference gene, and finally data statistical analysis was performed.

[0026] 5. Experimental Results and Analysis The skin lesions of the nodules are often round or oval, can be raised above the skin surface, or may not be raised, and feel a certain hardness when touched. The surface of the lung tissue of the mice in the blank group was smooth and soft; compared with the blank group, the surface of the lung tissue of the mice in the model group was significantly uneven, with round-like bulges and a certain hardness when touched; compared with the model group, the bulges on the surface of the lung tissue of the mice in Example 1, Example 2, and Example 3 were not obvious, and the number of nodules was significantly reduced.

[0027] As Figures 1 - 4 shown, compared with the blank group, the mRNA levels of TNF-α, IL-1β, IL-6, and IL-8 in the lung tissues of the mice in the model group were all significantly increased; the mRNA levels of TNF-α, IL-1β, IL-6, and IL-8 in the lung tissues of the mice in Example 1, Example 2, and Example 3 were significantly decreased ((P < 0.05 or P < 0.01).

[0028] The above are only embodiments of the present application and are not intended to limit the present application. For those skilled in the art, various changes and modifications can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the scope of the claims of the present application.

Claims

1. Application of American cockroach powder in medicine for the treatment of pulmonary sarcoidosis.

2. The use according to claim 1, characterized in that: The American cockroach powder is American cockroach Periplaneta americana (Linnaeus.) is a fine powder obtained by sterilizing, drying and then crushing.

3. A Chinese medicine composition for treating pulmonary nodules, characterized in that: The invention comprises the following raw materials in parts by weight: 3-9 parts of American cockroaches, 3-10 parts of astragalus, 3-10 parts of scutellaria, 5-10 parts of thunbergia thunbergii, 10-15 parts of selfheal, 3-9 parts of pinellia tuber, 5-10 parts of red peony root and 6-9 parts of oldenlandia diffusa.

4. The Chinese medicine composition according to claim 3, characterized in that: The invention comprises the following raw materials in parts by weight: 3 parts of American cockroach, 3 parts of astragalus, 3 parts of cyperus, 2 parts of pinellia tuber, 4 parts of selfheal, 2 parts of fritillaria thunbergii, 2 parts of red peony root and 2 parts of oldenlandia diffusa.

5. The Chinese medicine composition according to claim 3, characterized in that: The Chinese medicine composition is obtained by mixing American cockroaches with other Chinese medicines, and the other Chinese medicines are directly crushed into powder, or are made into powder after extraction, concentration and drying.

6. The Chinese medicine composition according to claim 5, characterized in that: The extraction solvent is water.

7. A medicine prepared from the Chinese medicine composition according to any one of claims 3 to 6.

8. The drug according to claim 7, characterized in that The medicine also includes medically or pharmaceutically acceptable excipients; the medicine can be in the form of powder, water pill, granule, tablet, capsule and the like.

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