A traditional Chinese medicine composition for improving allergic asthma and its preparation method

Abstract

This invention belongs to the field of respiratory disease drugs and fermentation engineering technology, specifically relating to a traditional Chinese medicine composition for improving allergic asthma and its preparation method. The traditional Chinese medicine composition for improving allergic asthma comprises the following components in parts by weight: Ephedra 10-20 parts, Earthworm 1-10 parts, Mulberry Bark 3-17 parts, Poria 6-17 parts, Schisandra Fruit 4-15 parts, Cinnamon Twig 8-18 parts, and Perilla Leaf 1-10 parts. This invention, through screening various high-quality traditional Chinese medicinal materials and rationally combining them, forms a traditional Chinese medicine composition with synergistic effects. This composition is refined through a specific preparation process, effectively preserving the active ingredients in the medicinal materials, and exhibits significant effects in treating and improving allergic asthma. This invention also has advantages such as high safety, few side effects, and suitability for long-term use.

Description

Technical Field

[0001] This invention belongs to the field of respiratory disease drugs and fermentation engineering technology, specifically relating to a traditional Chinese medicine composition for improving allergic asthma and its preparation method. Background Technology

[0002] Allergic asthma (also known as allergic wheezing) is a common respiratory disease. It is an IgE-mediated allergic asthma disease triggered by allergens. Susceptible individuals are prone to symptoms caused by inflammation, such as wheezing, shortness of breath, chest tightness, and coughing.

[0003] Treatment for respiratory diseases typically involves two different drug systems: Western medicine and traditional Chinese medicine. Currently, glucocorticoids (commonly known as "hormones") remain one of the main methods used in modern medicine to treat allergic asthma. However, with in-depth clinical research, it has been gradually discovered that long-term use of hormones may lead to a series of systemic or local adverse reactions. Allergic asthma is a chronic, recurrent respiratory disease. In its prevention and treatment, short-term symptom control is far from sufficient. Fundamentally regulating the patient's allergic constitution and enhancing the body's tolerance to external allergens is key to achieving long-term stable control and reducing relapses. Traditional Chinese medicine, while alleviating symptoms, focuses on adjusting the body's overall function and improving constitution. It can also serve as a supplement to Western medicine treatment, particularly in reducing the side effects of Western medications.

[0004] The application of fermentation engineering in traditional Chinese medicine extracts is an emerging and rapidly developing field. It combines the advantages of traditional Chinese medicine with modern biotechnology, aiming to increase the content of effective components, improve efficacy, reduce toxicity, and develop new drug resources. However, in current research and production practices of traditional Chinese medicine fermentation, the control of key parameters during the fermentation process is often not given sufficient attention. For example, factors such as temperature, pH, dissolved oxygen, inoculum size, fermentation time, and culture medium composition can significantly affect the metabolic activity of microorganisms and the formation of target products. Without systematic research and standardized operating procedures, batch-to-batch product quality instability, large fluctuations in efficacy, and even potential safety hazards may occur.

[0005] Therefore, there is an urgent need for a drug specifically targeting respiratory diseases such as allergic asthma. Summary of the Invention

[0006] The purpose of this invention is to provide a traditional Chinese medicine composition for improving allergic asthma and its preparation method.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] The first aspect of the present invention provides a traditional Chinese medicine composition for improving allergic asthma, comprising the following components in parts by weight: 10-20 parts of ephedra, 1-10 parts of earthworm, 3-17 parts of mulberry bark, 6-17 parts of poria cocos, 4-15 parts of schisandra chinensis, 8-18 parts of cinnamon twig, and 1-10 parts of perilla leaf.

[0009] Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 10-14 parts ephedra, 3-8 parts earthworm, 7-12 parts mulberry bark, 11-17 parts poria cocos, 10-15 parts schisandra chinensis, 8-12 parts cinnamon twig, and 1-5 parts perilla leaf.

[0010] Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: Ephedra 12 parts, Earthworm 5 parts, Mulberry bark 9 parts, Poria 14 parts, Schisandra chinensis 13 parts, Cinnamon twig 10 parts, and Perilla leaf 3 parts.

[0011] The principal herb is ephedra, which is pungent and warm, promotes lung function, dispels cold and relieves exterior symptoms, and plays a leading role in promoting lung qi and relieving airway spasms.

[0012] The assistant herb is earthworm, which unblocks the meridians and relieves spasms, and works synergistically with ephedra to alleviate respiratory distress; cinnamon twig warms and invigorates yang qi, and can also resolve phlegm.

[0013] The adjuvant herbs are mulberry bark, which clears heat and drains the lungs, resolves phlegm and stops cough; poria, which strengthens the spleen and promotes diuresis, cutting off the source of phlegm and fluid retention; and schisandra, which astringes the lungs and nourishes the kidneys, regulating the balance of opening and closing.

[0014] The guiding herb is perilla leaf, which gently disperses lung qi, dispels wind and releases the exterior, guides all the herbs upward to the lung meridian, and also harmonizes the properties of the herbs.

[0015] A second aspect of the present invention provides a method for preparing the traditional Chinese medicine composition for improving allergic asthma, comprising the following steps:

[0016] (1) Mix ephedra, earthworm and cinnamon twig and crush them. Add water and heat to soak. Cool down, add Lactobacillus delbrueckii and anaerobic ferment. After fermentation, sterilize and filter to obtain extract A. Concentrate extract A and spray dry to obtain extract A.

[0017] (2) Mix mulberry bark, schisandra fruit, poria cocos and perilla leaf and crush them. Add ethanol aqueous solution for extraction. After extraction, filter to obtain extract B1 and extract residue. Concentrate extract B1 and spray dry to obtain extract B1.

[0018] (3) Add water and red phaf yeast to the extraction residue of step (2), and perform aerobic fermentation. After fermentation, sterilize and filter to obtain extract B2. Concentrate extract B2 and spray dry to obtain extract B2.

[0019] (4) Mix extract A, extract B1 and extract B2 to obtain a traditional Chinese medicine composition for improving allergic asthma.

[0020] Preferably, the anaerobic fermentation conditions in step (1) are anaerobic fermentation at 35-40℃ for 30-40 hours.

[0021] Preferably, the dosage of Lactobacillus delbrueckii is 10. 7 -10 9 CFU / mL water.

[0022] More preferably, the dosage of Lactobacillus delbrueckii is 10. 8 CFU / mL water.

[0023] Preferably, in step (1), ephedra, earthworm, and cinnamon twig are mixed and then pulverized to a particle size of less than 60 mesh.

[0024] Preferably, in step (2), the volume concentration of the ethanol aqueous solution is 80-90%, and the extraction is carried out at 60-65℃ for 4-7 hours.

[0025] Preferably, in step (2), the mulberry bark, schisandra fruit, poria cocos, and perilla leaf are mixed and then pulverized to a particle size of less than 60 mesh.

[0026] Preferably, in step (3), the aerobic fermentation time is 25-30 hours, the fermentation temperature is 20-25°C, and the aeration rate is controlled at 1.0-1.3 vvm. vvm is a key parameter used in the fermentation industry to measure the aeration rate, which refers to the ratio of the volume of gas introduced into the fermenter per minute to the actual volume of liquid material in the tank, and its unit is cubic meters / (cubic meters * minutes).

[0027] Preferably, the amount of red phaf yeast used in step (3) is 10. 8 -10 10 CFU / mL water.

[0028] More preferably, the amount of red phaf yeast used in step (3) is 10. 9 CFU / mL water.

[0029] A third aspect of the present invention provides a pharmaceutical preparation for improving allergic asthma, the pharmaceutical preparation comprising the above-mentioned traditional Chinese medicine composition.

[0030] Preferably, the pharmaceutical preparation further includes pharmaceutically acceptable excipients.

[0031] Preferably, the excipients include one or more of the following: disintegrants, lubricants, fillers, flavoring agents, binders, sweeteners, and stabilizers.

[0032] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0033] 1. This invention selects various high-quality Chinese medicinal materials and, through rational formulation, forms a Chinese medicine composition with synergistic effects. The formulation principle of this invention is to combine warming and clearing herbs, and to balance dispersing and astringing. Through the rational combination of principal, assistant, adjuvant, and guide herbs, this invention fully utilizes the characteristics of each herb, achieving a comprehensive therapeutic effect from dispelling cold and relieving exterior syndromes, unblocking meridians and relieving spasms, clearing heat and resolving phlegm, to regulating yin and yang balance. It aims to fundamentally improve the patient's physical condition, alleviate symptoms, and prevent recurrence.

[0034] 2. The traditional Chinese medicine composition of this invention is refined through a specific preparation process, effectively preserving the active ingredients in the medicinal materials and enhancing the bioavailability and stability of the drug. Experimental verification has shown that this traditional Chinese medicine composition exhibits significant effects in treating and improving allergic asthma, effectively relieving airway inflammation, reducing airway hyperresponsiveness, and regulating immune function, thereby fundamentally improving asthma symptoms and reducing the frequency of attacks. Furthermore, this invention also has advantages such as high safety, few side effects, and suitability for long-term use, making it suitable for allergic asthma patients of different ages.

[0035] 3. This invention achieves precise control through optimization of the microbial strain and fermentation conditions. By precisely controlling key parameters such as temperature, pH, and dissolved oxygen during fermentation, the consistency and stability of product quality across batches can be ensured, avoiding differences in product efficacy caused by fluctuations in fermentation conditions. Accurate control of fermentation conditions maximizes the yield of the target active ingredients while promoting the generation of new active metabolites, thereby enhancing the overall efficacy of the traditional Chinese medicine extract and making the therapeutic effect more significant. This invention, by establishing a set of scientifically sound fermentation condition control standards, helps to advance the standardization process of traditional Chinese medicine fermentation production. Detailed Implementation

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] All raw materials used in the following embodiments of the present invention are commercially available products:

[0038] Lactobacillus delbrueckii, product number TS289314, Ningbo Taisto Biotechnology Co., Ltd.

[0039] Aspergillus oryzae, product number TS284177, Ningbo Taisto Biotechnology Co., Ltd.

[0040] Lactobacillus plantarum, catalog number TS279067, Ningbo Taisto Biotechnology Co., Ltd.

[0041] Red Pharf yeast, product number TS274931, Ningbo Taisto Biotechnology Co., Ltd.

[0042] Example 1

[0043] This embodiment provides a traditional Chinese medicine composition for improving allergic asthma, comprising the following components in parts by weight: 12 parts ephedra, 5 parts earthworm, 9 parts mulberry bark, 14 parts poria cocos, 13 parts schisandra chinensis, 10 parts cinnamon twig, and 3 parts perilla leaf.

[0044] The preparation method of the traditional Chinese medicine composition for improving allergic asthma includes the following steps:

[0045] (1) Mix ephedra, earthworm, and cinnamon twig and pulverize to less than 60 mesh to obtain mixture A. Add water to mixture A at a volume ratio of 22 mL: 1 g, heat to 95°C, and soak for 7 hours. Cool to 32°C and add Lactobacillus delbrueckii at a dosage of 10 g / L. 8 CFU / mL water, anaerobic fermentation at 37℃ for 36h, sterilization, filtration through a 0.45μm filter membrane to obtain extract A, concentration of extract A, and spray drying to obtain extract A;

[0046] (2) Mulberry bark, Schisandra chinensis, Poria cocos, and Perilla frutescens leaves were mixed and pulverized to obtain mixture B. An ethanol aqueous solution with a volume concentration of 85% was added and extracted at 62°C for 6 hours. The volume mass ratio of the ethanol aqueous solution to mixture A was 22 mL: 1 g. The mixture was filtered using a 0.45 μm filter membrane to obtain extract B1. After concentrating extract B1, it was spray-dried to obtain extract B1 and extract residue.

[0047] (3) Add water and red phaffeine yeast to the extraction residue. The amount of red phaffeine yeast used is 10. 9 The volume ratio of water to extract residue was 20 mL: 1 g. The aerobic fermentation time was 27 h, the fermentation temperature was 22 °C, and the aeration rate was controlled at 1.2 vvm. After sterilization, the extract was filtered through a 0.45 μm filter membrane to obtain extract B2. After concentration, extract B2 was spray-dried to obtain extract B2.

[0048] (4) Mix extract A, extract B1 and extract B2 to obtain a traditional Chinese medicine composition for improving allergic asthma.

[0049] Example 2

[0050] This embodiment provides a traditional Chinese medicine composition for improving allergic asthma, comprising the following components in parts by weight: 10 parts ephedra, 8 parts earthworm, 7 parts mulberry bark, 17 parts poria cocos, 10 parts schisandra chinensis, 12 parts cinnamon twig, and 1 part perilla leaf.

[0051] The preparation method of the traditional Chinese medicine composition for improving allergic asthma includes the following steps:

[0052] (1) Mix ephedra, earthworm, and cinnamon twig and pulverize to less than 60 mesh to obtain mixture A. Add water to mixture A at a volume ratio of 22 mL: 1 g, heat to 95°C, and soak for 6 hours. Cool to 35°C and add Lactobacillus delbrueckii at a dosage of 10 g / L. 8 CFU / mL water, anaerobic fermentation at 30℃ for 40h, sterilization, filtration through a 0.45μm filter membrane to obtain extract A, concentration of extract A, and spray drying to obtain extract A;

[0053] (2) Mix mulberry bark, schisandra fruit, poria cocos, and perilla leaf, then pulverize them to obtain mixture B. Add an 80% ethanol aqueous solution and extract at 65°C for 4 hours. The volume-to-mass ratio of the ethanol aqueous solution to mixture A is 22 mL: 1 g. Filter using a 0.45 μm filter membrane to obtain extract B1. Concentrate extract B1 and spray dry to obtain extract B1 and extract residue.

[0054] (3) Add water and red phaffeine yeast to the extraction residue. The amount of red phaffeine yeast used is 10. 9 The volume ratio of water to extract residue was 20 mL: 1 g. The aerobic fermentation time was 30 h, the fermentation temperature was 20 °C, and the aeration rate was controlled at 1.3 vvm. After sterilization, the extract was filtered through a 0.45 μm filter membrane to obtain extract B2. After concentration, extract B2 was spray-dried to obtain extract B2.

[0055] (4) Mix extract A, extract B1 and extract B2 to obtain a traditional Chinese medicine composition for improving allergic asthma.

[0056] Example 3

[0057] This embodiment provides a traditional Chinese medicine composition for improving allergic asthma, comprising the following components in parts by weight: 14 parts ephedra, 3 parts earthworm, 12 parts mulberry bark, 11 parts poria cocos, 15 parts schisandra chinensis, 8 parts cinnamon twig, and 5 parts perilla leaf.

[0058] The preparation method of the traditional Chinese medicine composition for improving allergic asthma includes the following steps:

[0059] (1) Mix ephedra, earthworm, and cinnamon twig and pulverize to less than 60 mesh to obtain mixture A. Add water to mixture A at a volume ratio of 22 mL: 1 g, heat to 95°C, and soak for 8 hours. Cool to 30°C and add Lactobacillus delbrueckii at a dosage of 10 g / L. 8CFU / mL water, anaerobic fermentation at 38℃ for 30h, sterilization, filtration through a 0.45μm filter membrane to obtain extract A, concentration of extract A, and spray drying to obtain extract A;

[0060] (2) Mulberry bark, Schisandra chinensis, Poria cocos, and Perilla frutescens leaves were mixed and pulverized to obtain mixture B. An ethanol aqueous solution with a volume concentration of 90% was added and extracted at 60°C for 7 hours. The volume mass ratio of the ethanol aqueous solution to mixture A was 22 mL: 1 g. The mixture was filtered using a 0.45 μm filter membrane to obtain extract B1. After concentrating extract B1, it was spray-dried to obtain extract B1 and extract residue.

[0061] (3) Add water and red phaffeine yeast to the extraction residue. The amount of red phaffeine yeast used is 10. 9 The volume ratio of water to extract residue was 20 mL: 1 g. The aerobic fermentation time was 25 h, the fermentation temperature was 25 °C, and the aeration rate was controlled at 1.0 vvm. After sterilization, the extract was filtered through a 0.45 μm filter membrane to obtain extract B2. After concentration, extract B2 was spray-dried to obtain extract B2.

[0062] (4) Mix extract A, extract B1 and extract B2 to obtain a traditional Chinese medicine composition for improving allergic asthma.

[0063] Comparative Example 1

[0064] The only difference between this comparative example and Example 1 is the ratio of the amounts of each component; all other conditions are the same, as detailed below:

[0065] The traditional Chinese medicine composition for improving allergic asthma includes the following components in parts by weight: 25 parts ephedra, 13 parts earthworm, 2 parts mulberry bark, 1 part poria cocos, 18 parts schisandra chinensis, 3 parts cinnamon twig, and 15 parts perilla leaf.

[0066] Comparative Example 2

[0067] The only difference between this comparative example and Example 1 is that the total mass of ephedra, earthworm, and cinnamon twig remains unchanged, while the mass ratio of cinnamon twig, ephedra, and earthworm remains unchanged, as detailed below:

[0068] The traditional Chinese medicine composition for improving allergic asthma includes the following components in parts by weight: 19 parts ephedra, 8 parts earthworm, 9 parts mulberry bark, 14 parts poria cocos, 13 parts schisandra chinensis, and 3 parts perilla leaf.

[0069] Comparative Example 3

[0070] The only difference between this comparative example and Example 1 is that the total mass of mulberry bark, poria cocos, and schisandra chinensis remains the same, but the mass ratio of the three is changed. Details are as follows:

[0071] A traditional Chinese medicine composition for improving allergic asthma includes the following components in parts by weight: Ephedra 12 parts, Earthworm 5 parts, Mulberry bark 14 parts, Poria 21 parts, Schisandra chinensis 1 part, Cinnamon twig 10 parts, and Perilla leaf 3 parts.

[0072] Comparative Example 4

[0073] The only difference between this comparative example and Example 1 is the preparation method. Details are as follows:

[0074] The preparation method of the traditional Chinese medicine composition for improving allergic asthma includes the following steps: Ephedra, earthworm, mulberry bark, Poria, Schisandra, cinnamon twig, and perilla leaf are mixed and pulverized to less than 60 mesh to obtain a mixture. Water is added to the mixture, with a volume-to-mass ratio of water to mixture A of 22 mL: 1 g. The mixture is heated to 95°C and soaked for 7 hours. The temperature is then lowered to 32°C, and Lactobacillus delbrueckii is added at a dosage of 10 g / ml. 8 The mixture was anaerobic fermented at 32°C for 36 hours with CFU / mL water, sterilized, and filtered through a 0.45μm filter membrane to obtain an extract. The extract was then concentrated and spray-dried to obtain a traditional Chinese medicine composition for improving allergic asthma.

[0075] Comparative Example 5

[0076] The only difference between this comparative example and Example 1 is that *Lactobacillus delbrueckii* is replaced with *Lactobacillus plantarum*. The amount of *Lactobacillus plantarum* used is 10... 8 CFU / mL water. All other conditions are the same.

[0077] Comparative Example 6

[0078] The only difference between this comparative example and Example 1 is that *Phaeophyte rubrum* is replaced with *Aspergillus oryzae*, and the amount of *Aspergillus oryzae* used is 10... 9 CFU / mL water. All other conditions are the same.

[0079] Performance testing

[0080] Establishing an ovalbumin (OVA)-induced asthma model: When the allergen ovalbumin is injected into mice, its soluble antigenic components stimulate the body to produce IgE, sensitizing the body. When the animals are exposed to this antigen again, an antigen-antibody reaction occurs mediated by IgE, causing cell degranulation and the release of active chemicals such as histamine and eosinophil chemotactic factors, which act on the bronchi to cause airway hyperresponsiveness, leading to asthma.

[0081] Female BALB / c mice (6-8 weeks old) weighing 18-22g SPF grade were selected. Mice were housed in an animal room with a room temperature maintained at 22±2℃ and a relative humidity of 50-60%. The light cycle was controlled by an automatic timer, with 07:00-19:00 as the dark period and 19:00-07:00 as the light period. Mice had free access to feed and sterile reverse osmosis water. Experiments were conducted only after the mice had acclimatized to their environment for 2 weeks. The experimental animals were randomly divided into groups of 6 mice each: Examples 1-3 and Comparative Examples 1-6 served as the sample group; the control group (not sensitized with OVA) and the model group (sensitized with OVA only, without treatment). The mice were then treated accordingly.

[0082] Animal model construction method: Sample group and model group: Sensitization was performed in each group via intraperitoneal injection on day 0 and day 14 of the experiment, with an injection dose of 0.2 mL OVA / animal (20 μg OVA + 2 mg aluminum hydroxide adjuvant mixture). Control group: 0.2 mL PBS / animal (PBS + 2 mg aluminum hydroxide adjuvant mixture) was injected intraperitoneally.

[0083] Mice in the model group and sample group were challenged by nebulization with 1wt% OVA on days 20, 21, 22, 23, 24 and 25, once a day for 25 minutes each time. The control group was nebulized with PBS in the same way.

[0084] Administration method: Starting from day 14, animals were treated by gavage. Administration was performed 1 hour before nebulization challenge. The administration method was as follows: the sample group was administered 0.30 g of the traditional Chinese medicine composition / kg body weight mixed with 0.2 mL of physiological saline via gavage. The control and model groups were administered the same volume of physiological saline via gavage. Three weeks after administration, mice were euthanized by injection of sodium pentobarbital at 240 mg / kg. The levels of IL-5, IL-13 in bronchoalveolar lavage fluid, and specific IgE antibodies in serum were measured. Experimental results are expressed as mean ± SD, and p < 0.05 was used to determine statistical significance.

[0085] The weight of the mice was measured daily during the experiment, and the weight of the mice was measured again before the end of the experiment. The weight of the mice at the end of the experiment was used as the experimental data, and the weight difference of the mice in each group was statistically analyzed.

[0086] The results are shown in Table 1.

[0087] Table 1. Results of testing the effects of traditional Chinese medicine compositions on a mouse asthma model.

[0088]

[0089] As can be seen from the data in Table 1, the traditional Chinese medicine compositions used in Examples 1-3 exhibit significant effects in reducing inflammatory responses and regulating immune responses. These compositions effectively reduced the expression levels of inflammatory cytokines, including IL-5 and IL-13, in bronchoalveolar lavage fluid. These two cytokines are crucial mediators in the pathological process of allergic asthma, and their reduction indicates that the drugs play an important role in alleviating airway inflammation and suppressing allergic reactions.

[0090] Furthermore, the experimental results showed that the traditional Chinese medicine compositions of Examples 1-3 could significantly reduce the concentration of specific IgE antibodies in plasma. IgE antibodies play a crucial role in allergic diseases, and elevated levels are generally positively correlated with the severity of allergic symptoms. Therefore, by reducing IgE antibody levels, this traditional Chinese medicine composition can not only alleviate the clinical symptoms of asthma patients but also help improve their overall quality of life and may reduce the frequency and intensity of asthma attacks.

[0091] Compared with the control group, the traditional Chinese medicine compositions in Examples 1-3 had no significant effect on the body weight of mice, indicating that the traditional Chinese medicine compositions at the experimental doses would not have a negative impact on the normal growth and development of mice.

[0092] The above experiments not only verified the effectiveness of the traditional Chinese medicine compositions in Examples 1-3 in treating allergic asthma, but also provided important clues for further understanding their mechanism of action. By regulating key inflammatory factors and immune indicators, this traditional Chinese medicine composition offers a new and potentially safer and more effective treatment for allergic asthma. This lays a solid foundation for further in-depth research and clinical application, and also provides valuable experience and direction for developing more novel therapies based on traditional Chinese medicine theory.

[0093] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine composition for improving allergic asthma, characterized in that, The traditional Chinese medicine composition consists of the following components in parts by weight: Ephedra 10-14 parts, Earthworm 3-8 parts, Mulberry Bark 7-12 parts, Poria 11-17 parts, Schisandra Fruit 10-15 parts, Cinnamon Twig 8-12 parts, and Perilla Leaf 1-5 parts.

2. The traditional Chinese medicine composition for improving allergic asthma according to claim 1, characterized in that, The traditional Chinese medicine composition consists of the following components in parts by weight: 12 parts ephedra, 5 parts earthworm, 9 parts mulberry bark, 14 parts poria cocos, 13 parts schisandra chinensis, 10 parts cinnamon twig, and 3 parts perilla leaf.

3. A method for preparing a traditional Chinese medicine composition for improving allergic asthma according to any one of claims 1-2, characterized in that, Includes the following steps: (1) Mix ephedra, earthworm and cinnamon twig and crush them. Add water and heat to soak. Cool down, add Lactobacillus delbrueckii and anaerobic ferment. After fermentation, sterilize and filter to obtain extract A. Concentrate extract A and spray dry to obtain extract A. (2) Mix mulberry bark, schisandra fruit, poria cocos and perilla leaf and crush them. Add ethanol aqueous solution and extract. After extraction, filter to obtain extract B1 and extract residue. Concentrate extract B1 and spray dry to obtain extract B1. (3) Add water and red phaf yeast to the extraction residue of step (2), and perform aerobic fermentation. After fermentation, sterilize and filter to obtain extract B2. Concentrate extract B2 and spray dry to obtain extract B2. (4) Mix extract A, extract B1 and extract B2 to obtain a traditional Chinese medicine composition for improving allergic asthma.

4. The method for preparing the traditional Chinese medicine composition for improving allergic asthma according to claim 3, characterized in that, The anaerobic fermentation conditions in step (1) are 35-40℃ for 30-40h.

5. The method for preparing the traditional Chinese medicine composition for improving allergic asthma according to claim 3, characterized in that, In step (2), the volume concentration of the ethanol aqueous solution is 80-90%, the extraction temperature is 60-65℃, and the extraction time is 4-7h.

6. The method for preparing the traditional Chinese medicine composition for improving allergic asthma according to claim 3, characterized in that, In step (3), the aerobic fermentation time is 25-30h, the fermentation temperature is 20-25℃, and the aeration rate is controlled at 1.0-1.3vvm.

7. A pharmaceutical preparation for improving allergic asthma, characterized in that, The pharmaceutical preparation is made from the traditional Chinese medicine composition according to any one of claims 1-2 and pharmaceutically acceptable excipients.

8. The pharmaceutical preparation according to claim 7, characterized in that, The excipients include at least one of the following: disintegrant, lubricant, filler, flavoring agent, binder, and stabilizer.

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