A polypeptide preparation system and preparation process for frozen prepared foods

Abstract

The present invention discloses a polypeptide preparation system and a preparation process for frozen prepared products, which relates to the technical field of polypeptide preparation. The system includes a box body, on which a rotating column is rotatably connected. A cavity is formed in the rotating column, and a water inlet groove is formed in the bottom inner wall of the cavity. The system further includes: a mixing mechanism located on the rotating column; the mixing mechanism includes a stirring unit, a driving unit, and a feeding unit. The stirring unit includes a floating block slidably sleeved on the rotating column. Two fixing blocks are fixedly connected to the bottom of the floating block. Rotating rods are rotatably connected to both of the two fixing blocks, and driven rods are rotatably connected to one ends of the two rotating rods. With the arrangement of the stirring unit in the present invention, the polypeptide solution can be stirred by the rotation of the rotating rods and the driven rods, and the longitudinal stirring range can be expanded, improving the stirring effect on the polypeptide solution.

Description

Technical Field

[0001] The present invention relates to the technical field of polypeptide preparation, and in particular to a polypeptide preparation system and preparation process for frozen prepared foods. Background Art

[0002] During the preparation of polypeptides, purification treatment is required, and the addition and mixing of the purification buffer solution for the polypeptide solution is a key step, which directly affects the pH stability of the solution and the purification effect of the polypeptide.

[0003] Chinese Patent with Publication No. CN221071371U discloses a heat dissipation device for polypeptide purification and preparation, including a processing box. A refrigerator is fixedly arranged on the inner wall of the bottom of the processing box through bolts. Two air outlets are connected to the top of the refrigerator. An air suction pipe is connected to one side of the refrigerator. The air suction pipe penetrates through the processing box. A filter screen is detachably installed on the inner wall of the end of the air suction pipe. A fixing plate is fixedly arranged on the inner wall of the processing box through bolts. A plurality of heating pipes are fixedly installed on the inner walls of both sides of the processing box through bolts. The positions of the heating pipes are adapted to the fixing plate. A plurality of heat dissipation openings are parallelly arranged on both sides of the processing box.

[0004] Based on the above search and in combination with the prior art, it is found that most of the existing stirring rods fixedly arrange a plurality of fixed rods longitudinally on the rotating shaft. When the solution to be purified is less, the stirring structure above the solution cannot participate in the stirring work, resulting in waste of stirring energy. When the solution to be purified is more, due to the fixed position of the stirring structure, the solution above the stirring rod cannot be fully stirred, and the mixing effect is poor, making it difficult for the traditional stirring rod to adapt to solutions of different volumes. Summary of the Invention

[0005] The purpose of the present invention is to provide a polypeptide preparation system and preparation process for frozen prepared foods to solve the problems raised in the above background art.

[0006] The technical solution of the present invention is: A polypeptide preparation system for frozen prepared foods includes a box body. A rotating column is rotatably connected to the box body. A cavity is opened in the rotating column. A water inlet groove is opened on the inner wall of the bottom of the cavity. It further includes:

[0007] A mixing mechanism, which is located on the rotating column;

[0008] The mixing mechanism includes a stirring unit, a driving unit, and a feeding unit. The stirring unit includes a floating block slidably sleeved on the rotating column. Two fixing blocks are fixedly connected to the bottom of the floating block. Rotating rods are rotatably connected to both of the two fixing blocks. One ends of the two rotating rods are respectively rotatably connected to driven rods. One ends of the two driven rods are respectively rotatably connected to mounting blocks. One ends of the two mounting blocks are fixedly connected to the rotating column. A one-way water inlet pipe is fixedly connected to the rotating column. The one-way water inlet pipe communicates with the water inlet groove. Two connecting pipes are fixedly communicated with the one-way water inlet pipe. The two connecting pipes are respectively rotatably connected to the two driven rods. A plurality of water absorption holes are formed in both of the two driven rods. A one-way water outlet pipe is fixedly connected to the rotating column. The one-way water outlet pipe communicates with the cavity. A sliding piston rod is slidably connected to the rotating column. The bottom end of the sliding piston rod extends into the cavity.

[0009] Preferably, the driving unit includes a fixing frame fixedly connected to the box body. A motor is fixedly connected to the fixing frame. The output end of the motor is fixedly connected to a driving shaft. The driving shaft is rotatably connected to the fixing frame. A rotating shaft is rotatably connected to the fixing frame. The mutually close ends of the rotating shaft and the driving shaft are jointly fixedly connected with a U-shaped rod. A long strip ring is sleeved on the U-shaped rod. An L-shaped rod is slidably connected to the fixing frame. The bottom end of the L-shaped rod is fixedly connected to the long strip ring. The other end of the L-shaped rod is rotatably sleeved on the sliding piston rod. A bevel gear is fixedly connected to one end of the rotating shaft and the rotating column respectively. The two bevel gears are meshed with each other.

[0010] Preferably, a telescopic spring is sleeved on the sliding piston rod. The two ends of the telescopic spring are respectively fixedly connected with the sliding piston rod and the rotating column.

[0011] Preferably, sliding sleeves are slidably sleeved on both of the two driven rods. Connecting rods are jointly rotatably connected to the two sliding sleeves and the two rotating rods respectively. A plurality of strip-shaped holes are formed in the sliding sleeves. The plurality of strip-shaped holes are respectively misaligned and overlapped with the plurality of water absorption holes.

[0012] Preferably, the feeding unit includes a storage bin fixedly connected to the top of the box body. A discharge pipe is fixedly connected to the bottom of the storage bin. The discharge pipe is fixedly connected to the box body. An activity pipe is slidably connected to the inner wall of the bottom end of the discharge pipe. Two feeding holes are formed in the activity pipe. A fixing handle is fixedly sleeved on the activity pipe. An activity sleeve is slidably sleeved on the rotating column. Two fixing rods are fixedly connected to the activity sleeve. A circular ring feeding plate is jointly fixedly connected to one ends of the two fixing rods. An extrusion block is fixedly connected to the circular ring feeding plate. When the extrusion block rotates circumferentially, it contacts with the fixing handle.

[0013] Preferably, a return spring is sleeved on the movable pipe, and two ends of the return spring are fixedly connected to the fixed handle and the discharge pipe respectively.

[0014] Preferably, a plurality of material leakage holes are fixedly connected to the bottom of the circular ring feeding plate, and the plurality of material leakage holes are distributed in a circular shape.

[0015] Preferably, two limiting strips are fixedly connected to the rotating column, the floating block is slidably sleeved on the two limiting strips, and the tops of the two limiting strips are in contact with the bottom end of the sliding sleeve.

[0016] Preferably, a feed pipe is fixedly communicated with the box body, and a discharge pipe is fixedly communicated with the bottom of the box body.

[0017] The present invention also provides a polypeptide preparation process for frozen prepared products, and the preparation process includes the following steps:

[0018] Step 1: Dissolve the polypeptide in a suitable solvent, and then filter the polypeptide solution using a 0.22 um filter membrane to remove particulate matters. If the polypeptide contains salts, desalination is required first.

[0019] Step 2: Put the filtered polypeptide solution into a purification box for purification. During the purification of the polypeptide solution, a buffer solution is added and stirred to ensure the stability of the pH value, and then separation is carried out using a chromatographic column.

[0020] Step 3: Finally, freeze-dry the purified polypeptide solution to obtain solid polypeptide, and store it in an environment of -20°C or -80°C.

[0021] The present invention provides a polypeptide preparation system and preparation process for frozen prepared products through improvement. Compared with the prior art, the following improvements and advantages are achieved:

[0022] First: Through the setting of the stirring unit in the present invention, the motor drives the drive shaft, the U-shaped rod and the rotating shaft to rotate. The rotation of the rotating shaft drives the rotating column to rotate through two bevel gears. The rotation of the rotating column drives the floating block, the fixed block, the rotating rod, the driven rod and the mounting block to perform circular rotation, so that the rotation of the rotating rod and the driven rod can stir the polypeptide solution. When the water level of the solution rises, it drives the floating block to move upward. Since the rotating rod and the driven rod can form a folding rod, the movement of the floating block drives the folding rod to stretch upward, so that the folding rod unfolds as the water level rises, thereby expanding the longitudinal stirring range and improving the stirring effect on the polypeptide solution.

[0023] Second: In the present invention, the rotation of the U-shaped rod drives the long strip ring and the L-shaped rod to reciprocate. The reciprocating movement of the L-shaped rod drives the sliding piston rod to reciprocate, so that the polypeptide solution in the box enters the driven rod through the water absorption holes, then enters the cavity through the connecting pipe, the one-way water inlet pipe and the water inlet tank, and finally flows back into the solution through the one-way water outlet pipe, enabling the polypeptide solution to circulate, improving the mixing effect of the polypeptide solution. When the folding rod is stretched upward, the rotating rod drives the sliding sleeve to move through the connecting rod. The movement of the sliding sleeve drives the movement of the strip-shaped hole, increasing the overlapping area between the strip-shaped hole and the water absorption hole, and further enabling the water absorption hole to increase as the solution increases, thereby being able to absorb more solution and improving the circulation effect of the solution.

[0024] Third: Through the setting of the feeding unit in the present invention, the rotation of the rotating column drives the movable sleeve, the fixed rod and the circular ring feeding plate to rotate. The rotation of the circular ring feeding plate drives the extrusion block to rotate circumferentially, so that the extrusion block presses the fixed handle to move upward once every rotation. The movement of the fixed handle drives the movable tube to move upward, enabling the feeding hole of the movable tube to enter the discharge pipe. At this time, the buffer solution in the storage tank flows into the circular ring feeding plate through the discharge pipe and the movable tube, and finally flows into the polypeptide solution. When there is more solution, the floating block moves upward to press the movable sleeve, the fixed rod, the circular ring feeding plate and the extrusion block to move upward, increasing the distance that the extrusion block drives the fixed handle and the movable tube to move upward. Since the feeding hole is strip-shaped, the area of the feeding hole entering the discharge pipe increases, increasing the liquid discharge amount of the buffer solution, realizing that when the polypeptide solution increases, the liquid discharge amount of the buffer solution increases accordingly, enabling the buffer solution to be proportioned with the polypeptide solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the following drawings are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0026] Figure 1 It is a schematic three-dimensional structure diagram of the whole in the present invention;

[0027] Figure 2 It is a schematic three-dimensional sectional structure diagram inside the box in the present invention;

[0028] Figure 3 It is a schematic three-dimensional structure diagram of the stirring unit in the present invention;

[0029] Figure 4 It is a schematic three-dimensional structure diagram of the driving unit in the present invention;

[0030] Figure 5Schematic three-dimensional structure diagram of the extrusion block and the fixed handle in the present invention;

[0031] Figure 6 Internal cross-sectional plan structure diagram of the discharge pipe in the present invention;

[0032] Figure 7 Internal cross-sectional plan structure diagram of the rotating column in the present invention.

[0033] Reference numerals:

[0034] 1, box body; 11, rotating column; 12, floating block; 13, fixed block; 14, rotating rod; 15, driven rod; 16, water absorption hole; 17, mounting block; 18, sliding sleeve; 19, strip-shaped hole; 110, connecting rod; 2, cavity; 21, water inlet groove; 22, one-way water inlet pipe; 23, one-way water outlet pipe; 24, connecting pipe; 25, sliding piston rod; 26, telescopic spring; 3, fixed frame; 31, motor; 32, drive shaft; 33, U-shaped rod; 34, rotating shaft; 35, bevel gear; 36, long strip ring; 37, L-shaped rod; 4, movable sleeve; 41, fixed rod; 42, circular ring feed plate; 43, extrusion block; 44, leakage hole; 5, storage tank; 51, discharge pipe; 52, movable pipe; 53, fixed handle; 54, feed hole; 55, return spring; 6, feed pipe; 7, discharge pipe; 8, limiting strip. Detailed implementation manners

[0035] The present invention will be described in detail below. The technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0036] The present invention provides a polypeptide preparation system and a preparation process for frozen prepared foods through improvement. The technical solution of the present invention is as follows:

[0037] Embodiment 1:

[0038] As Figures 1 to 7 shown, the embodiment of the present invention provides a polypeptide preparation system for frozen prepared foods, including a box body 1, a rotating column 11 is rotatably connected to the box body 1, a cavity 2 is opened in the rotating column 11, a water inlet groove 21 is opened on the bottom inner wall of the cavity 2, and further includes:

[0039] A mixing mechanism, the mixing mechanism is located on the rotating column 11;

[0040] The mixing mechanism includes a stirring unit, a driving unit, and a feeding unit. The stirring unit includes a floating block 12 slidably sleeved on a rotating column 11. Two fixing blocks 13 are fixedly connected to the bottom of the floating block 12. Rotating rods 14 are rotatably connected to both of the two fixing blocks 13. One ends of the two rotating rods 14 are rotatably connected to driven rods 15. One ends of the two driven rods 15 are rotatably connected to mounting blocks 17. One ends of the two mounting blocks 17 are fixedly connected to the rotating column 11. A one-way water inlet pipe 22 is fixedly connected to the rotating column 11. The one-way water inlet pipe 22 is communicated with a water inlet groove 21. Two connecting pipes 24 are fixedly communicated with the one-way water inlet pipe 22. The two connecting pipes 24 are respectively rotatably connected to the two driven rods 15. A plurality of water absorption holes 16 are formed in both of the two driven rods 15. A one-way water outlet pipe 23 is fixedly connected to the rotating column 11. The one-way water outlet pipe 23 is communicated with a cavity 2. A sliding piston rod 25 is slidably connected to the rotating column 11. The bottom end of the sliding piston rod 25 extends into the cavity 2; through the arrangement of the stirring unit, the driving unit drives the rotating column 11 to rotate. The rotation of the rotating column 11 drives the floating block 12, the fixing blocks 13, the rotating rods 14, the driven rods 15, and the mounting blocks 17 to perform circular rotation, so that the rotation of the rotating rods 14 and the driven rods 15 can stir the polypeptide solution. When the water level of the solution rises, it drives the floating block 12 to move upward. Since the rotating rods 14 and the driven rods 15 can form a folding rod, the movement of the floating block 12 drives the folding rod to stretch upward, so that the folding rod unfolds as the water level rises, thereby expanding the longitudinal stirring range and improving the stirring effect on the polypeptide solution. When the sliding piston rod 25 reciprocates, the polypeptide solution in the box body 1 enters the driven rod 15 through the water absorption holes 16, then enters the cavity 2 through the connecting pipes 24, the one-way water inlet pipe 22, and the water inlet groove 21, and finally flows back into the solution through the one-way water outlet pipe 23, so that the polypeptide solution circulates, improving the mixing effect on the polypeptide solution.

[0041] Further, the driving unit includes a fixing frame 3 fixedly connected to the box body 1. A motor 31 is fixedly connected to the fixing frame 3. The output end of the motor 31 is fixedly connected to a driving shaft 32. The driving shaft 32 is rotatably connected to the fixing frame 3. A rotating shaft 34 is rotatably connected to the fixing frame 3. The ends of the rotating shaft 34 and the driving shaft 32 close to each other are fixedly connected to a U-shaped rod 33 together. A long strip ring 36 is sleeved on the U-shaped rod 33. An L-shaped rod 37 is slidably connected to the fixing frame 3. The bottom end of the L-shaped rod 37 is fixedly connected to the long strip ring 36. The other end of the L-shaped rod 37 is rotatably sleeved on the sliding piston rod 25. Conical gears 35 are fixedly connected to one end of the rotating shaft 34 and the rotating column 11 respectively. The two conical gears 35 are meshed with each other. Through the setting of the driving unit, the motor 31 drives the driving shaft 32, the U-shaped rod 33 and the rotating shaft 34 to rotate. The rotation of the rotating shaft 34 drives the rotating column 11 to rotate through the two conical gears 35. At the same time, the rotation of the U-shaped rod 33 drives the long strip ring 36 and the L-shaped rod 37 to reciprocate. The reciprocating movement of the L-shaped rod 37 drives the sliding piston rod 25 to reciprocate, realizing power output.

[0042] Further, a telescopic spring 26 is sleeved on the sliding piston rod 25. The two ends of the telescopic spring 26 are fixedly connected to the sliding piston rod 25 and the rotating column 11 respectively. Through the setting of the telescopic spring 26, the reciprocating movement of the sliding piston rod 25 drives the telescopic spring 26 to expand and contract, thereby improving the stability of the sliding piston rod 25.

[0043] Further, as Figure 3 shown, sliding sleeves 18 are slidably sleeved on the two driven rods 15. Connecting rods 110 are rotatably connected to the two sliding sleeves 18 and the two rotating rods 14 respectively. A plurality of strip-shaped holes 19 are formed in the sliding sleeves 18. The plurality of strip-shaped holes 19 are misaligned and overlapped with the plurality of water absorption holes 16 respectively. Through the setting of the sliding sleeves 18, when the folding rod is stretched upward, the rotating rod 14 drives the sliding sleeve 18 to move through the connecting rod 110. The movement of the sliding sleeve 18 drives the strip-shaped holes 19 to move, increasing the overlapping area of the strip-shaped holes 19 and the water absorption holes 16. Thus, the water absorption holes 16 can increase as the solution increases, so that more solution can be absorbed, improving the circulation effect of the solution.

[0044] Further, as Figures 5 to 6As shown in the figure, the feeding unit includes a storage tank 5 fixedly connected to the top of the box body 1. The bottom of the storage tank 5 is fixedly connected with a discharge pipe 51. The discharge pipe 51 is fixedly connected to the box body 1. The inner wall of the bottom end of the discharge pipe 51 is slidably connected with a movable pipe 52. Two feeding holes 54 are formed in the movable pipe 52. A fixed handle 53 is fixedly sleeved on the movable pipe 52. A movable sleeve 4 is slidably sleeved on the rotating column 11. Two fixed rods 41 are fixedly connected to the movable sleeve 4. One ends of the two fixed rods 41 are fixedly connected together with a circular ring feeding plate 42. An extrusion block 43 is fixedly connected to the circular ring feeding plate 42. When the extrusion block 43 rotates circumferentially, it contacts the fixed handle 53. Through the setting of the feeding unit, the rotation of the rotating column 11 drives the rotation of the movable sleeve 4, the fixed rods 41 and the circular ring feeding plate 42. The rotation of the circular ring feeding plate 42 drives the extrusion block 43 to rotate circumferentially, so that the extrusion block 43 presses the fixed handle 53 to move upward once every rotation. The movement of the fixed handle 53 drives the movable pipe 52 to move upward, so that the feeding holes 54 of the movable pipe 52 enter the discharge pipe 51. At this time, the buffer solution in the storage tank 5 flows into the circular ring feeding plate 42 through the discharge pipe 51 and the movable pipe 52, and finally flows into the polypeptide solution. When the solution is more, the floating block 12 moves upward to press the movable sleeve 4, the fixed rods 41, the circular ring feeding plate 42 and the extrusion block 43 to move upward, so that the distance that the extrusion block 43 drives the fixed handle 53 and the movable pipe 52 to move upward increases. Since the feeding holes 54 are strip-shaped, the area of the feeding holes 54 entering the discharge pipe 51 increases, so as to increase the liquid discharge amount of the buffer solution, realizing that when the polypeptide solution increases, the liquid discharge amount of the buffer solution increases accordingly, so that the buffer solution can be proportioned with the polypeptide solution.

[0045] Further, as Figure 5 shown, a return spring 55 is sleeved on the movable pipe 52. Two ends of the return spring 55 are respectively fixedly connected with the fixed handle 53 and the discharge pipe 51. Through the setting of the return spring 55, when the extrusion block 43 is away from the fixed handle 53, the return spring 55 can drive the fixed handle 53 and the movable pipe 52 to reset.

[0046] Further, a plurality of leakage holes 44 are fixedly connected to the bottom of the circular ring feeding plate 42. The plurality of leakage holes 44 are annularly distributed. Through the setting of the leakage holes 44, the buffer solution of the circular ring feeding plate 42 can fall into the polypeptide solution through the leakage holes 44, improving the purification effect of the polypeptide solution.

[0047] Further, as Figures 2 to 3 shown, two limiting strips 8 are fixedly connected to the rotating column 11. The floating block 12 is slidably sleeved on the two limiting strips 8. The top ends of the two limiting strips 8 are in contact with the bottom end of the sliding sleeve 18. Through the setting of the limiting strips 8, the stability of the floating block 12 is improved.

[0048] Further, a feed pipe 6 is fixedly connected to the box body 1, and a discharge pipe 7 is fixedly connected to the bottom of the box body 1; through the arrangement of the feed pipe 6, the polypeptide solution can be fed, and through the arrangement of the discharge pipe 7, the purified solution can be discharged.

[0049] Example Two:

[0050] This example also discloses a polypeptide preparation process for frozen ready-to-eat products, and this preparation process includes the following steps:

[0051] Step One: Dissolve the polypeptide in an appropriate solvent, and then filter the polypeptide solution using a 0.22 um filter membrane to remove particulate matter. If the polypeptide contains salts, desalination is required first;

[0052] Step Two: Place the filtered polypeptide solution into a purification box for purification. Add a buffer solution and stir during the purification process of the polypeptide solution to ensure the stability of the pH value, and then use a chromatographic column for separation;

[0053] Step Three: Finally, lyophilize the purified polypeptide solution to obtain solid polypeptide, and store it in an environment of -20°C or -80°C.

[0054] Specific implementation steps: Introduce the polypeptide solution into the box body 1 through the feed pipe 6. At the same time, start the motor 31 to drive the drive shaft 32, U-shaped rod 33 and rotating shaft 34 to rotate. The rotation of the rotating shaft 34 drives the rotating column 11 to rotate through two bevel gears 35. The rotation of the rotating column 11 drives the floating block 12, fixed block 13, rotating rod 14, driven rod 15 and mounting block 17 to rotate in a circle, so that the rotation of the rotating rod 14 and the driven rod 15 can stir the polypeptide solution. When the water level of the solution rises, it drives the floating block 12 to move upward. Since the rotating rod 14 and the driven rod 15 can form a folding rod, the movement of the floating block 12 drives the folding rod to stretch upward, so that the folding rod unfolds as the water level rises, thereby expanding the longitudinal stirring range and improving the stirring effect on the polypeptide solution. The rotation of the U-shaped rod 33 drives the long strip ring 36 and the L-shaped rod 37 to move reciprocally. The reciprocal movement of the L-shaped rod 37 drives the sliding piston rod 25 to move reciprocally. When the sliding piston rod 25 moves reciprocally, the polypeptide solution in the box body 1 enters the driven rod 15 through the water absorption hole 16, then enters the cavity 2 through the connecting pipe 24, one-way water inlet pipe 22 and water inlet groove 21, and finally flows back into the solution through the one-way water outlet pipe 23, making the polypeptide solution circulate, improving the mixing effect on the polypeptide solution. When the folding rod stretches upward, the rotating rod 14 drives the sliding sleeve 18 to move through the connecting rod 110. The movement of the sliding sleeve 18 drives the strip hole 19 to move, so that the overlapping area of the strip hole 19 and the water absorption hole 16 increases, and thus the water absorption hole 16 can increase as the solution increases, so as to absorb more solution and improve the circulation effect of the solution. The rotation of the rotating column 11 drives the movable sleeve 4, fixed rod 41 and ring feed plate 42 to rotate. The rotation of the ring feed plate 42 drives the extrusion block 43 to rotate in a circle, so that the extrusion block 43 presses the fixed handle 53 to move upward once every rotation. The fixed handle 53 drives the return spring 55 to compress and undergo elastic deformation. At the same time, the movement of the fixed handle 53 drives the movable tube 52 to move upward, so that the feed hole 54 of the movable tube 52 enters the discharge pipe 51. At this time, the buffer solution in the storage tank 5 flows into the ring feed plate 42 through the discharge pipe 51 and the movable tube 52, and then falls into the solution through the leakage hole 44. When the solution is more, the floating block 12 moves upward to press the movable sleeve 4, fixed rod 41, ring feed plate 42 and extrusion block 43 to move upward, so that the extrusion block 43 drives the fixed handle 53 and the movable tube 52 to move upward by a greater distance. Since the feed hole 54 is strip-shaped, the area of the feed hole 54 entering the discharge pipe 51 increases, thereby increasing the liquid discharge amount of the buffer solution. When the polypeptide solution increases, the liquid discharge amount of the buffer solution increases accordingly, so that the buffer solution can be proportioned with the polypeptide solution.

[0055] The foregoing description enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A polypeptide preparation system for frozen prepared foods, comprising a box body (1), characterized in that: A rotating column (11) is rotatably connected to the box body (1). A cavity (2) is formed in the rotating column (11). A water inlet groove (21) is formed in the bottom inner wall of the cavity (2). Further included are: A mixing mechanism, which is located on the rotating column (11); The mixing mechanism includes a stirring unit, a driving unit and a feeding unit. The stirring unit includes a floating block (12) slidably sleeved on the rotating column (11). Two fixing blocks (13) are fixedly connected to the bottom of the floating block (12). Rotating rods (14) are rotatably connected to both of the two fixing blocks (13). Driven rods (15) are rotatably connected to one ends of the two rotating rods (14). Mounting blocks (17) are rotatably connected to one ends of the two driven rods (15). One ends of the two mounting blocks (17) are fixedly connected to the rotating column (11). A one-way water inlet pipe (22) is fixedly connected to the rotating column (11). The one-way water inlet pipe (22) is communicated with the water inlet groove (21). Two connecting pipes (24) are fixedly communicated with the one-way water inlet pipe (22). The two connecting pipes (24) are respectively rotatably connected to the two driven rods (15). A plurality of water absorption holes (16) are formed in both of the two driven rods (15). A one-way water outlet pipe (23) is fixedly connected to the rotating column (11). The one-way water outlet pipe (23) is communicated with the cavity (2). A sliding piston rod (25) is slidably connected to the rotating column (11). The bottom end of the sliding piston rod (25) extends into the cavity (2); The driving unit includes a fixing frame (3) fixedly connected to the box body (1). A motor (31) is fixedly connected to the fixing frame (3). A driving shaft (32) is fixedly connected to the output end of the motor (31). The driving shaft (32) is rotatably connected to the fixing frame (3). A rotating shaft (34) is rotatably connected to the fixing frame (3). A U-shaped rod (33) is fixedly connected to the mutually close ends of the rotating shaft (34) and the driving shaft (32). A long strip ring (36) is sleeved on the U-shaped rod (33). An L-shaped rod (37) is slidably connected to the fixing frame (3). The bottom end of the L-shaped rod (37) is fixedly connected to the long strip ring (36). The other end of the L-shaped rod (37) is rotatably sleeved on the sliding piston rod (25). Bevel gears (35) are fixedly connected to one end of the rotating shaft (34) and the rotating column (11). The two bevel gears (35) are meshed with each other; The feeding unit includes a storage bin (5) fixedly connected to the top of the box body (1). A discharge pipe (51) is fixedly connected to the bottom of the storage bin (5), and the discharge pipe (51) is fixedly connected to the box body (1). An activity pipe (52) is slidably connected to the inner wall of the bottom end of the discharge pipe (51). Two feeding holes (54) are formed in the activity pipe (52). A fixed handle (53) is fixedly sleeved on the activity pipe (52). An activity sleeve (4) is slidably sleeved on the rotating column (11). Two fixed rods (41) are fixedly connected to the activity sleeve (4). One ends of the two fixed rods (41) are jointly fixedly connected to a circular ring feeding plate (42). An extrusion block (43) is fixedly connected to the circular ring feeding plate (42). When the extrusion block (43) rotates circumferentially, it contacts the fixed handle (53).

2. A polypeptide preparation system for frozen prepared products according to claim 1, characterized in that: A telescopic spring (26) is sleeved on the sliding piston rod (25). Two ends of the telescopic spring (26) are respectively fixedly connected to the sliding piston rod (25) and the rotating column (11).

3. The polypeptide preparation system for frozen prepared foods according to claim 2, wherein: Sliding sleeves (18) are slidably sleeved on the two driven rods (15). A connecting rod (110) is jointly rotatably connected to the two sliding sleeves (18) and the two rotating rods (14). A plurality of strip-shaped holes (19) are formed in the sliding sleeves (18). The plurality of strip-shaped holes (19) are respectively misaligned and overlapped with the plurality of water absorption holes (16).

4. A polypeptide preparation system for frozen prepared foods according to claim 3, characterized in that: A reset spring (55) is sleeved on the activity pipe (52). Two ends of the reset spring (55) are respectively fixedly connected to the fixed handle (53) and the discharge pipe (51).

5. A polypeptide preparation system for frozen prepared foods according to claim 3, characterized in that: A plurality of leakage holes (44) are fixedly connected to the bottom of the circular ring feeding plate (42). The plurality of leakage holes (44) are annularly distributed.

6. A polypeptide preparation system for frozen prepared foods according to claim 3, characterized in that: Two limit strips (8) are fixedly connected to the rotating column (11). The floating block (12) is slidably sleeved on the two limit strips (8). Tops of the two limit strips (8) contact the bottom end of the sliding sleeve (18).

7. A polypeptide preparation system for frozen prepared foods according to claim 6, characterized in that: A feeding pipe (6) is fixedly communicated with the box body (1). A discharge pipe (7) is fixedly communicated with the bottom of the box body (1).

8. A preparation process for polypeptides for frozen prepared foods, characterized in that, Applicable to a polypeptide preparation system for frozen prepared products as described in any one of claims 1-7, the preparation process includes the following steps: Step 1: Dissolve the polypeptide in an appropriate solvent, then filter the polypeptide solution using a 0.22 um filter membrane to remove particulate matter. If the polypeptide contains salts, it needs to be desalted first. Step 2: Put the filtered polypeptide solution into a purification box for purification. Add a buffer solution and stir during the purification process of the polypeptide solution to ensure the stability of the pH value, and then use a chromatographic column for separation. Step 3: Finally, freeze-dry the purified polypeptide solution to obtain solid polypeptide, and store it in an environment of -20°C or -80°C.

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