Aminobutyric acid and polysaccharide biological regulator and preparation method thereof

Abstract

The invention relates to the technical field of biological regulators, and provides an aminobutyric acid and polysaccharide biological regulator and a preparation method thereof. The biological regulator is composed of an aminobutyric acid purified crude product, a polysaccharide crude product and kaolin powder modified by a silane coupling agent, and aminobutyric acid and polysaccharide biological regulator particles are obtained through mixing granulation. Besides, the aminobutyric acid and polysaccharide biological regulator is combined with a coating agent formed by carboxymethyl chitosan, ground calcium carbonate and Tween 20 and an outer layer prepared from biomass ash, dried livestock and poultry manure and gelatin, and the composite slow release fertilizer with a three-layer structure is formed through spray granulation, drying and curing. Therefore, plant growth regulation and slow-release fertilizer supply are realized.

Description

Technical Field

[0001] The present invention relates to the technical field of biological regulators. More specifically, the present invention relates to a gamma-aminobutyric acid and polysaccharide biological regulator and a preparation method thereof. Background Art

[0002] In recent years, biological regulators such as gamma-aminobutyric acid and polysaccharides have become a research hotspot in the field of green agriculture due to their functional characteristics of enhancing crop stress resistance and promoting nutrient absorption. For example, gamma-aminobutyric acid can improve the anti-saline-alkali ability by regulating plant metabolism, while polysaccharides can promote the formation of soil aggregate structure and activate beneficial microbial communities. However, the preparation processes of existing biological regulators mostly rely on simple physical mixing or direct granulation. The active ingredients are easily degraded rapidly due to the influence of environmental humidity, microbial decomposition, etc., and the conventional carrier materials have low adsorption efficiency due to surface inertness, severely restricting their field persistence period and large-scale application value.

[0003] Meanwhile, agricultural production still mainly relies on chemically synthesized nitrogen fertilizers or compound fertilizers containing only nitrogen, phosphorus, and potassium. Such chemical fertilizers have a single function and lack slow-release performance, resulting in too fast nutrient release and insufficient crop absorption rate. Long-term use is likely to cause problems such as soil compaction. Although some slow-release fertilizers improve the release performance through synthetic resin coating technology, their materials are non-degradable, the preparation process is complex, and it is difficult to meet the release requirements of bioactive ingredients, unable to meet the dual requirements of green agriculture for functional integration and ecological compatibility.

[0004] To break through the above limitations, existing technologies have tried to combine biological regulators with slow-release fertilizers, but the contradiction of insufficient synergy between the two has become prominent. The physical compounding process has weak interfacial binding force, easily leading to the separation of active ingredients from fertilizer particles; while the dense structure of traditional coating materials can control the release of nitrogen, phosphorus, and potassium, but it hinders the diffusion of active substances such as gamma-aminobutyric acid and polysaccharides. Therefore, how to prepare a highly stable biological regulator and construct a multi-layer synergistic slow-release compound fertilizer with this as the core has become a technical challenge with both agricultural value and industrial feasibility. Summary of the Invention

[0005] In order to overcome the above-mentioned defects of the prior art, the present invention provides a gamma-aminobutyric acid and polysaccharide biological regulator and a preparation method thereof. By combining and modifying kaolin with two active substances, gamma-aminobutyric acid and polysaccharides, a gamma-aminobutyric acid and polysaccharide biological regulator is formed, and further combined with a coating agent composed of carboxymethyl chitosan and calcium carbonate and a biomass fertilizer composed of biomass ash and livestock manure to construct a composite slow-release system. Through material modification and structural design, the load stability and release controllability of active ingredients are simultaneously improved, solving the technical problems of active ingredient loss in traditional processes and the single effect and weak controlled release ability of composite slow-release fertilizers.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] An aminobutyric acid and polysaccharide biological regulator, wherein the aminobutyric acid and polysaccharide biological regulator comprises the following raw materials: crude purified aminobutyric acid, crude polysaccharide, kaolin powder, and the kaolin powder is modified kaolin powder, and the modified kaolin powder is obtained by subjecting kaolin powder to ultrasonic treatment in a mixed solution of deionized water and ethanol and modifying it with a silane coupling agent.

[0008] As a further scheme of the present invention, the modified kaolin powder is prepared by the following steps:

[0009] Add kaolin powder to a mixed solution of deionized water and ethanol, adjust the pH value of the solution with acetic acid, subject the solid-liquid mixture to ultrasonic treatment to fully mix the kaolin powder with the solution, then slowly drop the silane coupling agent and continue ultrasonic stirring. After stopping the ultrasonic treatment, stir in a reaction kettle, filter the reacted kaolin powder by suction, wash it with ethanol, and dry it for standby.

[0010] As a further scheme of the present invention, add kaolin powder to a mixed solution of deionized water and ethanol, and the volume ratio is 1:9; the pH value is 3.5 - 4; the time condition of the ultrasonic treatment is 20 min; after slowly dropping the silane coupling agent, ultrasonic stir for 5 min; after stopping the ultrasonic treatment, stir and react in a reaction kettle at 90 - 100 °C for 120 minutes; after the reaction is completed, perform suction filtration and wash with ethanol with a volume fraction of 50%.

[0011] The modified kaolin powder is more helpful for the binding of aminobutyric acid in the pore structure. First, mix the modified kaolin powder with the crude aminobutyric acid, and then add the crude polysaccharide and mix and granulate under the condition of spraying a small amount of water. It can prevent the problem of uneven distribution of polysaccharide, aminobutyric acid and kaolin powder due to the excessive viscosity of polysaccharide when it meets water.

[0012] As a further scheme of the present invention, the aminobutyric acid and polysaccharide biological regulator is further used as the core layer, coated with a coating agent composed of carboxymethyl chitosan, heavy calcium carbonate and Tween 20, and combined with a biological fertilizer composed of biomass ash, dried livestock and poultry manure and gelatin as the outer layer to obtain an aminobutyric acid and polysaccharide biological composite slow-release fertilizer.

[0013] As a further scheme of the present invention, the composite slow-release fertilizer has a three-layer structure, with the aminobutyric acid and polysaccharide biological regulator as the core layer, the coating agent as the middle layer, and the biological fertilizer as the outer layer.

[0014] As a further solution of the present invention, the weight parts of each raw material in the compound slow-release fertilizer are: 30-80 parts of amino butyric acid and polysaccharide biological regulator, 20-45 parts of coating agent, and 20-90 parts of biomass fertilizer; the component ratio of the compound slow-release fertilizer is amino butyric acid and polysaccharide biological regulator: coating agent: biomass fertilizer is mixed in a ratio of 3:2:5.

[0015] The weight parts of each raw material in the amino butyric acid and polysaccharide biological regulator are: 5-30 parts of crude purified amino butyric acid, 10-20 parts of crude polysaccharide, and 7-25 parts of kaolin powder; the kaolin powder is modified kaolin powder, and the modified kaolin powder is obtained by subjecting kaolin powder to ultrasonic treatment in a mixed solution of deionized water and ethanol and modifying it with a silane coupling agent; the component ratio of the amino butyric acid and polysaccharide biological regulator is: crude purified amino butyric acid powder: crude polysaccharide powder: modified kaolin powder is mixed in a ratio of 4:2:4.

[0016] The weight parts of each raw material in the coating agent are: 8-24 parts of carboxymethyl chitosan, 10-30 parts of heavy calcium carbonate, and 5-10 parts of Tween 20; the component ratio of the coating agent is: carboxymethyl chitosan: heavy calcium carbonate: Tween 20 is mixed in a ratio of 50:45:5.

[0017] The weight parts of each raw material in the biomass fertilizer are: 20-40 parts of biomass ash, 30-60 parts of dried livestock and poultry manure, and 5-10 parts of gelatin; the component ratio of the biomass fertilizer is: biomass ash: dried livestock and poultry manure: gelatin is mixed in a ratio of 40:50:10.

[0018] As a further solution of the present invention, a preparation method of an amino butyric acid and polysaccharide biological regulator includes the following steps:

[0019] Prepare the crude purified amino butyric acid, crude polysaccharide, and modified kaolin into powder mixtures, wherein the modified kaolin powder is first mixed with the crude amino butyric acid, and then mixed and granulated with the crude polysaccharide under the condition of spraying a small amount of water to obtain amino butyric acid and polysaccharide biological regulator granules through granulation.

[0020] As a further solution of the present invention, the mass fraction of the crude purified amino butyric acid is 40-60%, and the mass fraction of the crude polysaccharide is 20-35%.

[0021] As a further solution of the present invention, the crude purified amino butyric acid is made through the following steps:

[0022] Filter impurities from the microbial fermentation broth containing aminobutyric acid, add dilute hydrochloric acid to adjust the pH to 4.5 - 6.5, heat the transformed filtrate at 80 °C for 30 minutes, add anhydrous ethanol with a volume of 10% of the filtrate and stir continuously. Then add a flocculant to the transformation solution and stir continuously until the flocculant is completely dissolved. Let it stand for 30 min and perform pressure filtration to remove impurities;

[0023] Concentrate the supernatant after impurity removal by low-temperature vacuum freezing. Take it out when there is no obvious liquid solution. Dry the obtained crystals and collect them to finally obtain the crude product of γ-aminobutyric acid;

[0024] The crude product of aminobutyric acid is derived from microbial fermentation, and the strains used are Bacillus subtilis, lactic acid bacteria or Escherichia coli.

[0025] As a further scheme of the present invention, the crude polysaccharide purification product is prepared through the following steps:

[0026] Filter impurities from the microbial fermentation broth containing polysaccharides. Concentrate the filtrate to half of the original volume through an ultrafiltration membrane. Add 95% anhydrous ethanol with a volume half of the concentrated solution and stir while adding. Wait until white flocculates completely precipitate, perform plate and frame pressure filtration, and collect the solid matter for drying to obtain the crude polysaccharide product;

[0027] The crude polysaccharide product is derived from microbial fermentation, and the strains used are Leuconostoc mesenteroides, Enterobacter, Escherichia coli, Bacillus subtilis or lactic acid bacteria.

[0028] As a further scheme of the present invention, the aminobutyric acid and polysaccharide biological regulator can further be used as the core layer, and combined with an intermediate layer coating agent and a biomass fertilizer as the outer layer to prepare an aminobutyric acid and polysaccharide bio-composite slow-release fertilizer, including the following steps:

[0029] Step S1, dissolve carboxymethyl chitosan, heavy calcium carbonate, and Tween 20 in water to obtain a mixed coating agent solution. Then place the aminobutyric acid and polysaccharide biological regulator particles in a spray drum granulator. Under the rotating state, spray the mixed coating agent solution on the surface of the aminobutyric acid and polysaccharide biological regulator particles, dry and solidify to obtain the core biological regulator particles;

[0030] Step S2, mix biomass ash, dried livestock and poultry manure with gelatin and break them into biomass fertilizer powder. Add the core biological regulator particles and the biomass fertilizer powder into a spray drum granulator. Under the rotating state, spray xanthan gum aqueous solution. Stop granulation after the formation of particles, dry and solidify to obtain the aminobutyric acid and polysaccharide composite slow-release fertilizer.

[0031] As a further solution of the present invention, in step S1, the solid mass fraction of the coating agent mixture is 40 - 80%; the temperature condition for drying and curing is 60 - 80°C; the particle size of the core biological regulator particles is 2 - 3 mm.

[0032] As a further solution of the present invention, in step S2, the mixing ratio of the biomass ash and the dried livestock manure is 4:6; the addition ratio of the core biological regulator particles to the biomass fertilizer powder is 4:1; the mass concentration of the xanthan gum aqueous solution is 5%; the particle size of the formed particles is 0.4 - 0.6 cm; the temperature condition for drying and curing is 40 - 60°C.

[0033] The obtained γ-aminobutyric acid and polysaccharide biological regulator has an obvious three-layer structure: with γ-aminobutyric acid and polysaccharides as the core, the coating composed of carboxymethyl chitosan and heavy calcium carbonate as the middle layer, and the biomass fertilizer as the outer layer. This three-layer structure design ensures that the fertilizer can continuously release nutrients and growth regulators during use, improves the fertilizer utilization efficiency, and reduces environmental pollution at the same time.

[0034] Compared with the prior art, the beneficial effects of a γ-aminobutyric acid and polysaccharide biological regulator and its preparation method of the present invention are as follows:

[0035] The present invention provides a γ-aminobutyric acid and polysaccharide biological regulator, and constructs a three-layer composite slow-release fertilizer based on the biological regulator. With γ-aminobutyric acid and polysaccharides as regulatory factors to form the core layer, coated with a coating agent mainly composed of chitosan and calcium carbonate to form a controlled-release middle layer, and then compounded with biomass ash and livestock manure to form a nutrient outer layer. This structure can achieve continuous release of nutrients during the crop growth cycle, and at the same time stably release γ-aminobutyric acid and polysaccharides, playing a dual role of regulation and supply. Most of the existing slow-release fertilizers are single-nutrient control types, lacking structural stratification and functional coordination, and the release of regulatory substances is discontinuous, making it difficult to meet the requirements of plant nutrition and growth regulation at the same time.

[0036] The present invention uses kaolin modified by ethanol-aqueous solution cleaning, ultrasonic pretreatment and high-temperature reaction with a silane coupling agent as the regulator carrier, which can significantly enhance its binding ability with γ-aminobutyric acid, and optimize the internal component distribution of the particles by the method of mixing first and then granulating, effectively avoiding the problem of uneven distribution caused by the large viscosity of polysaccharides when encountering water. Most of the existing kaolin is directly blended and used in biological regulators without surface modification treatment, resulting in poor adsorption ability and unstable binding with regulatory factors, thereby affecting the uniformity and persistence of regulator release. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 It is a schematic structural diagram of the γ-aminobutyric acid and polysaccharide composite slow-release fertilizer of the present invention.

[0038] Figure 2This is a comparison chart of the growth status of wheat under different fertilization treatments of the present invention. Detailed implementation manners

[0039] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0040] A preparation method of a gamma-aminobutyric acid and polysaccharide biological regulator includes the following steps:

[0041] Prepare the crude gamma-aminobutyric acid purification product, crude polysaccharide product, and modified kaolin into powders. First, mix the modified kaolin powder with the crude gamma-aminobutyric acid product, then add the crude polysaccharide product, and perform mixing granulation under the condition of spraying a small amount of water. After granulation, obtain the gamma-aminobutyric acid and polysaccharide biological regulator granules.

[0042] Furthermore, a preparation method of a compound slow-release fertilizer containing gamma-aminobutyric acid and polysaccharide includes the following steps:

[0043] Step S1: Prepare the crude gamma-aminobutyric acid purification product, crude polysaccharide product, and modified kaolin into powders. First, mix the modified kaolin powder with the crude gamma-aminobutyric acid product, then add the crude polysaccharide product, and perform mixing granulation under the condition of spraying a small amount of water, and prepare granules with a particle size of 2 - 3 mm by extrusion. After granulation, obtain the gamma-aminobutyric acid and polysaccharide biological regulator granules;

[0044] Step S2: Prepare the carboxymethyl chitosan and heavy calcium carbonate into powders and dissolve them in water. Continuously stir and drop Tween 20 to obtain a coating agent mixture solution with a solid mass fraction of 40 - 80%. Then, place the gamma-aminobutyric acid and polysaccharide biological regulator granules in a spray rotary drum granulator. Under the rotating state, spray the coating agent mixture solution on the surface of the gamma-aminobutyric acid and polysaccharide biological regulator granules, and dry and cure at 60 - 80 °C to obtain core biological regulator granules with a particle size of 2 - 3 mm;

[0045] Step S3: Mix biomass ash and dried livestock manure in a ratio of 4:6 and break them into biomass fertilizer powder. Add the core biological regulator granules and the biomass fertilizer powder to a spray rotary drum granulator in a ratio of 4:1. Dissolve xanthan gum in water to prepare a 5% xanthan gum aqueous solution. Under the rotating state, spray the 5% xanthan gum aqueous solution. Stop granulation after forming 0.4 - 0.6 cm particles, and dry and cure at 40 - 60 °C to obtain a compound slow-release fertilizer containing gamma-aminobutyric acid and polysaccharide.

[0046] The amino-butyric acid and polysaccharide composite slow-release fertilizer described above comprises the following raw materials in parts by weight: 30 - 80 parts of amino-butyric acid and polysaccharide biological regulator, 20 - 45 parts of coating agent, and 20 - 90 parts of biomass fertilizer.

[0047] The amino-butyric acid and polysaccharide biological regulator described above comprises the following raw materials in parts by weight: 5 - 30 parts of crude purified amino-butyric acid, 10 - 20 parts of crude polysaccharide, and 7 - 25 parts of kaolin powder.

[0048] The coating agent described above comprises the following raw materials in parts by weight: 8 - 24 parts of carboxymethyl chitosan, 10 - 30 parts of heavy calcium carbonate, and 5 - 10 parts of Tween 20.

[0049] The biomass fertilizer described above comprises the following raw materials in parts by weight: 20 - 40 parts of biomass ash, 30 - 60 parts of dried livestock manure, and 5 - 10 parts of gelatin.

[0050] Example 1

[0051] In the embodiment of the present invention, the amino-butyric acid and polysaccharide composite slow-release fertilizer is prepared based on the amino-butyric acid and polysaccharide biological regulator, including using the combination of two active substances, amino-butyric acid and polysaccharide, to modify kaolin as the amino-butyric acid and polysaccharide biological regulator, using carboxymethyl chitosan and heavy calcium carbonate to form an active coating layer, and simultaneously using biomass ash and dried livestock manure as the biomass fertilizer layer. Under the action of gelatin, the adhesiveness of the overall structure is enhanced, and the slow-release effect of the fertilizer is further improved. Finally, a composite slow-release fertilizer is realized, with the active substances amino-butyric acid and polysaccharide as plant growth regulators and the biomass fertilizer providing nutrients.

[0052] In the embodiment of the present invention, the preparation of the amino-butyric acid and polysaccharide composite slow-release fertilizer first requires pretreatment of each raw material.

[0053] The crude purified amino-butyric acid in the embodiment of the present invention is prepared through the following steps: filtering the microbial fermentation broth containing amino-butyric acid to remove impurities such as cells, adding dilute hydrochloric acid to adjust the pH to 5, heating the conversion filtrate at 80 °C for 30 minutes, adding 10% volume of absolute ethanol to the filtrate and stirring continuously, adding a flocculant to the conversion liquid and stirring continuously until the flocculant is completely dissolved, standing for 30 min, and performing pressure filtration to remove impurities; concentrating the supernatant after impurity removal by low-temperature vacuum freezing, and taking it out when there is no obvious liquid solution; fully drying the obtained crystals and collecting them to finally obtain the crude γ-aminobutyric acid.

[0054] The crude purified polysaccharide in the embodiment of the present invention is prepared through the following steps: filtering the microbial fermentation broth containing polysaccharide to remove impurities such as cells, concentrating the filtrate to half of the original volume through an ultrafiltration membrane, adding 95% absolute ethanol with a volume half of the concentrated liquid, adding while stirring, waiting for the white floccules to completely precipitate, performing plate and frame filtration, and collecting the solid matter for drying to obtain the crude polysaccharide.

[0055] The kaolin powder in the embodiment of the present invention is prepared through the following steps: Add kaolin powder to a mixed solution of deionized water and ethanol with a volume ratio of 1:9. Use acetic acid to adjust the pH of the solution to about 3.5, and ultrasonically treat the solid-liquid mixture for 20 min to fully mix the kaolin powder with the solution. Subsequently, slowly drop a silane coupling agent and stir and ultrasonically treat for 5 min. After stopping the ultrasonic treatment, stir in a reaction kettle at 90 °C for 120 min. Filter the kaolin powder after the reaction is completed, wash it with 50% ethanol, and then dry it to obtain the modified kaolin powder.

[0056] After completing the raw material pretreatment process, the present invention further proceeds with the preparation process of the intermediate product.

[0057] In the embodiment of the present invention, 40 Kg of crude purified gamma-aminobutyric acid is fully mixed with 20 Kg of modified kaolin powder, and then 40 Kg of crude polysaccharide powder is added, and granulation is carried out under the condition of spraying a small amount of water. And extrude to prepare particles with a particle size of 2-4 mm. In this step, the kaolin powder needs to be modified and first mixed with the crude gamma-aminobutyric acid, and then mixed and granulated with the crude polysaccharide under the condition of spraying a small amount of water, because polysaccharides have viscosity, and such an order can prevent the problem of uneven distribution with gamma-aminobutyric acid and kaolin powder due to excessive viscosity of polysaccharides when they encounter water.

[0058] 50 Kg of carboxymethyl chitosan and 45 Kg of heavy calcium carbonate are mixed and dissolved in 60 mL of water, and 5 Kg of Tween 20 is continuously stirred and added dropwise to obtain a coating agent mixed solution with a solid mass fraction of 40-80%. Then, the gamma-aminobutyric acid and polysaccharide biological regulator particles are placed in a spray rotary drum granulator, and while rotating, the coating agent mixed solution is sprayed on the surface of the gamma-aminobutyric acid and polysaccharide biological regulator particles, and dried and cured at 70 °C to obtain core biological regulator particles with a particle size of 2-4 mm.

[0059] On the basis of completing the preparation of the intermediate product, the present invention proceeds with the synthesis process of the final compound slow-release fertilizer.

[0060] 40 Kg of biomass ash and 60 Kg of dry livestock and poultry manure are mixed and crushed into biomass fertilizer powder. Subsequently, the core biological regulator particles and the biomass fertilizer powder are added to a spray rotary drum granulator at a ratio of 4:1. Dissolve xanthan gum in water to prepare a 5% xanthan gum aqueous solution, and while rotating, spray the 5% xanthan gum aqueous solution. Stop granulation after 0.5 cm particles are formed, and dry and cure at 60 °C to obtain the gamma-aminobutyric acid and polysaccharide compound slow-release fertilizer.

[0061] The obtained gamma-aminobutyric acid and polysaccharide compound slow-release fertilizer has an obvious three-layer structure: the core layer is composed of gamma-aminobutyric acid and polysaccharides, the intermediate layer is the coating composed of carboxymethyl chitosan and heavy calcium carbonate, and the outer layer is the biomass fertilizer.

[0062] In the embodiment of the present invention, the γ-aminobutyric acid and polysaccharide composite slow-release fertilizer has γ-aminobutyric acid and polysaccharide as the core layer, the coating agent constitutes the intermediate layer, and the fortified biomass fertilizer forms the outer layer. This structural design ensures that during the slow-release process of the fertilizer, it can continuously provide nutrients for plants while maintaining the stable release of the growth regulator γ-aminobutyric acid and polysaccharide.

[0063] The present invention uses modified kaolin as an adjuvant for the γ-aminobutyric acid and polysaccharide composite slow-release fertilizer. First, deionized water and an ethanol mixed solution with a volume ratio of 1:9 are added to the kaolin powder. The pH of the solution is adjusted to about 3.5 - 4 using acetic acid, and the solid-liquid mixture is ultrasonically treated for 20 min to fully mix the kaolin powder with the solution, maximizing the removal of void impurities in the powder. Subsequently, a silane coupling agent is slowly added dropwise and stirred ultrasonically for 5 min. After stopping the ultrasonic treatment, it is heated in a reaction kettle to 90 - 100 °C and stirred for 120 min for modification treatment. The modified kaolin powder is more conducive to the binding of γ-aminobutyric acid in the pore structure. The modified kaolin powder is first mixed with the crude γ-aminobutyric acid, and then the crude polysaccharide is added and mixed granulated under the condition of spraying a small amount of water. This process can prevent the problem of uneven distribution of γ-aminobutyric acid, kaolin powder due to the excessive viscosity of polysaccharide when it meets water.

[0064] The effect of the γ-aminobutyric acid and polysaccharide composite slow-release fertilizer on wheat growth was tested through experiments. The sample prepared in Example 1 was used for wheat cultivation tests. Equal amounts of the blank, core biological regulator granules, the biomass fertilizer of this patent, ordinary compound fertilizer, and the compound fertilizer of this patent were applied, and the germination and growth status of 20 wheat seeds were observed and analyzed after 30 days of cultivation. The results showed that after using the core biological regulator granules, the biomass fertilizer of this patent, and the compound fertilizer of this patent in the experiments of this patent, the growth of wheat seedlings was much higher than that of the seedlings cultivated without fertilization and with ordinary compound fertilizer. The detailed data of the wheat germination rate are shown in Table 1:

[0065] Table 1

[0066]

[0067] As Figure 2 shown, observing the test results from left to right are the blank group, the core biological regulator granule group, the biomass fertilizer group of this patent, the ordinary compound fertilizer group, and the compound fertilizer group of this patent. The experiment shows that the compound fertilizer has a better effect.

[0068] Data analysis shows that the γ-aminobutyric acid and polysaccharide composite slow-release fertilizer described in this patent not only has the highest germination rate, but also the plant growth status is significantly better than other groups, fully proving the technical effect of the present invention. The high germination rate (90%) of the core biological regulator granule group indicates the effectiveness of γ-aminobutyric acid and polysaccharide as biological regulators, and the complete three-layer structure compound fertilizer further improves the overall effect through the slow-release mechanism, reaching a germination rate of 95%.

[0069] In summary, this embodiment proves that the compound slow-release fertilizer of gamma-aminobutyric acid and polysaccharide has significant effects in promoting plant growth. Its three-layer structure design of core-coating-outer layer enables it to continuously release active ingredients slowly, showing obvious advantages compared with traditional chemical fertilizers.

[0070] The above is only the specific implementation manner of this application, but the protection scope of this application is not limited thereto. Any person skilled in the art within the technical scope disclosed in this application can easily think of changes or substitutions, which should all be covered within the protection scope of this application. Therefore, the protection scope of this application shall be subject to the protection scope of the claims.

[0071] Finally: The above is only the preferred embodiment of the present invention and is not used to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An aminobutyric acid and polysaccharide bioregulator, characterized in that: The aminobutyric acid and polysaccharide bioregulator comprises the following raw materials: purified crude aminobutyric acid, crude polysaccharide, and kaolin powder, wherein the kaolin powder is modified kaolin powder, and the modified kaolin powder is prepared by ultrasonically treating the kaolin powder in a mixed solution of deionized water and ethanol and modifying it with a silane coupling agent, and comprises the following steps: Add kaolin powder into a mixed solution of deionized water and ethanol, use acetic acid to adjust the pH value of the solution, use ultrasonic treatment on the solid-liquid mixture to fully mix the kaolin powder and the solution, then slowly drop silane coupling agent and continue ultrasonic stirring. After stopping the ultrasound, stir in the reactor, filter the kaolin powder after the reaction, wash with ethanol and dry for later use.

2. The aminobutyric acid and polysaccharide bioregulator according to claim 1, characterized in that: The kaolin powder is added to a mixed solution of deionized water and ethanol in a volume ratio of 1:9; the pH value is 3.5-4; the ultrasonic treatment time condition is 20 minutes; after slowly dropping the silane coupling agent, ultrasonic stirring is performed for 5 minutes; after stopping the ultrasound, stirring the reaction in a reactor at 90-100° C. for 120 minutes; after the reaction is completed, suction filtration is performed and washing is performed with 50% volume fraction ethanol.

3. The aminobutyric acid and polysaccharide bioregulator according to claim 1, characterized in that: The aminobutyric acid and polysaccharide bioregulators can be further used as a core layer, coated by a coating agent composed of carboxymethyl chitosan, heavy calcium carbonate and Tween 20, and combined with biomass fertilizer composed of biomass ash, dry livestock and poultry manure and gelatin as an outer layer to prepare a biocomposite slow-release fertilizer containing aminobutyric acid and polysaccharide.

4. The aminobutyric acid and polysaccharide bioregulator according to claim 3, characterized in that: The composite slow-release fertilizer has a three-layer structure, with aminobutyric acid and polysaccharide bioregulators as the core layer, a coating agent as the middle layer, and biomass fertilizer as the outer layer; The weight proportions of the raw materials in the composite slow-release fertilizer are: 30-80 parts of aminobutyric acid and polysaccharide bioregulator, 20-45 parts of coating agent, and 20-90 parts of biomass fertilizer; The weight proportions of the raw materials in the aminobutyric acid and polysaccharide bioregulator are: 5-30 parts of purified crude aminobutyric acid, 10-20 parts of crude polysaccharide and 7-25 parts of kaolin powder; The weight proportions of the raw materials in the coating agent are: 8-24 parts of carboxymethyl chitosan, 10-30 parts of heavy calcium carbonate and 5-10 parts of Tween 20; The weight proportions of the raw materials in the biomass fertilizer are: 20-40 parts of biomass ash, 30-60 parts of dry livestock and poultry manure and 5-10 parts of gelatin.

5. A method for preparing the aminobutyric acid and polysaccharide bioregulator according to any one of claims 1 to 4, characterized in that: The following steps are involved: Purified crude aminobutyric acid, crude polysaccharide and modified kaolin are prepared into powders and mixed; The modified kaolin powder is first mixed with the crude aminobutyric acid product, and then mixed with the crude polysaccharide product under the condition of spraying a small amount of water to granulate to obtain the aminobutyric acid and polysaccharide bioregulator granules.

6. The method for preparing an aminobutyric acid and polysaccharide bioregulator according to claim 5, characterized in that: The mass fraction of the purified crude aminobutyric acid is 40-60%, and the mass fraction of the crude polysaccharide is 20-35%.

7. The method for preparing an aminobutyric acid and polysaccharide bioregulator according to claim 5, characterized in that: The purified crude aminobutyric acid product is prepared by the following steps: Filter the fermentation liquid of the microorganism containing aminobutyric acid to remove impurities, add dilute hydrochloric acid to adjust the pH to 4.5-6.5, heat the conversion filtrate at 80°C for 30 minutes, add 10% of the volume of anhydrous ethanol to the filtrate and stir continuously, then add a flocculant to the conversion liquid, stir continuously until the flocculant is completely dissolved, let it stand for 30 minutes, and perform filter pressing to remove impurities; The supernatant after impurities removal is concentrated by low-temperature vacuum freezing, and taken out when there is no obvious liquid solution; the obtained crystals are dried and collected to finally obtain crude γ-aminobutyric acid; The crude aminobutyric acid product is derived from microbial fermentation, and the strain used is Bacillus subtilis, lactic acid bacteria or Escherichia coli; The polysaccharide purified crude product is prepared by the following steps: The microbial fermentation liquid containing polysaccharides is filtered to remove impurities, the filtrate is concentrated to half of the original volume through an ultrafiltration membrane, 95% anhydrous ethanol is added in an amount of half the volume of the concentrate, and stirring is performed while adding until white flocs are completely precipitated, and the solid matter is collected and dried to obtain a crude polysaccharide; The crude polysaccharide is derived from microbial fermentation, and the strains used are Leuconostoc mesenteroides, Enterobacter, Escherichia coli, Bacillus subtilis or lactic acid bacteria.

8. The method for preparing an aminobutyric acid and polysaccharide bioregulator according to claim 5, characterized in that: The aminobutyric acid and polysaccharide biological regulators can be further used as a core layer, combined with a coating agent as an intermediate layer and a biomass fertilizer as an outer layer to prepare a biological composite slow-release fertilizer containing aminobutyric acid and polysaccharide, comprising the following steps: Step S1, dissolving carboxymethyl chitosan, heavy calcium carbonate and Tween 20 in water to obtain a coating agent mixture, and then placing aminobutyric acid and polysaccharide bioregulator particles in a spray drum granulator, spraying the coating agent mixture on the surface of the aminobutyric acid and polysaccharide bioregulator particles while rotating, and drying and solidifying to obtain core bioregulator particles; Step S2, mixing biomass ash, dry livestock and poultry manure and gelatin and crushing them into biomass fertilizer powder, adding the core bioregulator particles and the biomass fertilizer powder into a spray drum granulator, spraying the xanthan gum aqueous solution while the machine is rotating, stopping the granulation after the particles are formed, drying and solidifying, and obtaining a composite slow-release fertilizer of aminobutyric acid and polysaccharide.

9. The method for preparing an aminobutyric acid and polysaccharide bioregulator according to claim 8, characterized in that: In step S1, the solid mass fraction of the coating agent mixture is 40-80%; the temperature condition of the drying and curing is 60-80°C; and the particle size of the core bioregulator particles is 2-3mm.

10. The method for preparing an aminobutyric acid and polysaccharide bioregulator according to claim 8, characterized in that: In step S2, the addition ratio of the core bioregulator particles to the biomass fertilizer powder is 4:1; the mass concentration of the xanthan gum aqueous solution is 5%; the particle size of the formed particles is 0.4-0.6 cm; and the temperature condition for the drying and curing is 40-60°C.

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