Biostimulant and application thereof in promoting pesticide absorption of crops

Abstract

The invention belongs to the technical field of biological control, and particularly relates to a biostimulant and application thereof in promoting pesticide absorption of crops. The biostimulant disclosed by the invention comprises thymol and amino acid, wherein the amino acid is particularly tyrosine. When the biostimulant and pesticide are used for seed dressing treatment, the absorption efficiency of the rice on the pesticide can be obviously improved, the pesticide dosage is greatly reduced, and the influence of the pesticide on the ecological environment is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological control, and particularly relates to a biostimulant and its application in promoting the absorption of pesticides by crops. Background Art

[0002] Rice is the main staple food in China, and its yield is related to national food security. In recent years, due to climate change and the transformation of cultivation methods, sheath blight has occurred severely in most rice-growing areas in China, seriously affecting rice production. At present, the use of pesticides is still the main prevention and control measure for sheath blight. Under the background of the national action plan for reducing pesticide use, it is necessary to ensure that rice production is not affected, while continuously reducing the amount of pesticide use and the impact of pesticide use on the environment and ecology. It is urgent to continuously improve pesticide use technology and improve the accuracy and efficiency of pesticide use.

[0003] Seed treatment is an efficient and simple pesticide application technology. Especially for pests and diseases in the early and middle stages of rice growth such as sheath blight, it can achieve early prevention, effectively reduce the diffusion of pesticides in the environment, and reduce environmental impact. Pesticide seed treatment can play a systemic disease control role through plant absorption and conduction, thereby achieving long-term prevention and control of pests and diseases. Discovering compounds that can improve the absorption and transport efficiency of pesticides, improving the accuracy of pesticide use, and enhancing the utilization efficiency of pesticides is of great significance for the sustainable and healthy development of the rice industry.

[0004] Biostimulants are a general term for a large class of environmentally friendly compounds, which play an important role in promoting crop growth and development, improving plant physiological and biochemical status, enhancing crop root activity, regulating leaf stomatal opening, and enhancing crop stress resistance. The long-distance transport of pesticides is closely related to plant physiological and biochemical characteristics. Amino acids and thymol are common types of biostimulants. Research has found that after adding compound amino acids for seed dressing, the average contents of chlorantraniliprole in the stems and leaves of rice can be increased by 48-88% and 12-34%, respectively, proving that amino acids have great application potential in improving the utilization rate of chlorantraniliprole in rice. However, the key amino acid types that play the function of promoting pesticide absorption are not yet clear, and it is still unclear whether amino acids can promote the absorption of sheath blight control agents. Thymol is a natural small molecule compound that has been widely used in the fields of medicine and food preservation. Thymol not only has a significant function of inhibiting fungi, but also can regulate crops to resist abiotic stress, but it is not yet clear whether it has a mitigating effect on pesticide stress and a promoting effect on pesticide absorption. Summary of the Invention

[0005] Objective of the Invention: The technical problem to be solved by the present invention is to provide a biostimulant and its application in promoting the absorption of pesticides by crops in view of the deficiencies of the prior art. The biostimulant of the present invention can alleviate the stress of pesticides on rice, promote the growth of rice, promote the accumulation of various sheath blight control agents in the above-ground part of rice, and significantly improve the utilization efficiency of pesticides.

[0006] To solve the above technical problems, the present invention discloses a biostimulant and its application in promoting the absorption of pesticides by crops. The specific technical solutions are as follows:

[0007] An application of a biostimulant in promoting the absorption of pesticides by crops, wherein the biostimulant includes thymol.

[0008] Among them, the crop is rice.

[0009] Among them, the pesticide is a sheath blight control drug.

[0010] Among them, the sheath blight control drug includes any one or a combination of thifluzamide, azoxystrobin, or difenoconazole.

[0011] Among them, the biostimulant includes amino acids. Preferably, the amino acid is tyrosine.

[0012] Among them, in the biostimulant, the mass ratio of thymol to amino acids is 1:5 to 5:1. Preferably, the mass ratio of thymol to amino acids is 1:0.9 to 1.2.

[0013] Among them, the method for the biostimulant to promote the absorption of pesticides by crops is: after mixing the biostimulant with the pesticide, perform seed dressing on the crop seeds to promote the absorption of pesticides by the crops. Among them, the usage amount of thymol is 0.07 to 0.4 mg / g dry seeds. The usage amount of thymol is preferably 0.3 mg / g dry seeds.

[0014] In the second aspect, the present invention also provides a biostimulant, which includes thymol and amino acids. The amino acid is preferably tyrosine.

[0015] Beneficial Effects:

[0016] Compared with the prior art, the present invention provides a biostimulant combination formula. Using this formula for seed dressing treatment with pesticides can significantly improve the absorption efficiency of pesticides by rice, greatly reduce the dosage of pesticides, and reduce the impact of pesticides on the ecological environment. The specific effects are as follows:

[0017] 1) The biostimulant combination provided by the present invention has an obvious synergistic effect. When it is used for seed dressing together with the pesticides for controlling sheath blight, the absorption efficiency of thifluzamide by rice can be greatly improved, and the improvement ratio reaches 32.2% - 63.2%.

[0018] 2) In addition to being effective for thifluzamide, the biostimulant combination also has a good promoting effect on the absorption of commonly used pesticides for controlling sheath blight such as difenoconazole and azoxystrobin, and the improvement ratio reaches 38.2% - 49.4%.

[0019] Generally speaking, taking the main pesticides for controlling sheath blight as the object, the present invention first screens out the amino acid tyrosine with the best promoting effect on pesticide absorption, and then uses the compound of tyrosine and thymol for seed dressing. It is found for the first time that the compound biostimulant significantly promotes the accumulation of various pesticides in the above-ground parts of rice, realizes the improvement of pesticide utilization rate, and has important application value in ensuring the safe production of rice. Description of the Drawings

[0020] The following further specifically describes the present invention in conjunction with the drawings and specific embodiments, and the above and / or other advantages of the present invention will become clearer.

[0021] Figure 1 It shows the effects of 20 amino acids on the growth of rice under soil culture conditions. Among them, CK: control; Gly: glycine; Ala: alanine; Val: valine; Leu: leucine; Ile: isoleucine; Met: methionine; Pro: proline; Trp: tryptophan; Ser: serine; Tyr: tyrosine; Cys: cysteine; Phe: phenylalanine; Asn: asparagine; Gln: glutamine; Thr: threonine; Asp: aspartic acid; Glu: glutamic acid; Lys: lysine; Arg: arginine; His: histidine. Figure 1 A shows the effect on the plant height of rice, Figure 1 B shows the effect on the fresh weight of the above-ground parts of rice. Different lowercase letters in the figure represent significant differences between different amino acid treatment groups at P < 0.05.

[0022] Figure 2 It shows the effects of six amino acids on the fresh weight of the above-ground parts of rice and the accumulation of thifluzamide. Among them, CK: control; Glu: glutamic acid; Arg: arginine; Gln: glutamine; Ser: serine; Asn: asparagine; Tyr: tyrosine. Figure 2 A shows the effect on the fresh weight of the above-ground parts of rice; Figure 2 B shows the effect on the thifluzamide content in the above-ground parts of rice. Different lowercase letters in the figure represent significant differences between different amino acid treatment groups at P < 0.05.

[0023] Figure 3Effect of tyrosine addition amount on fresh weight of aboveground parts of rice and accumulation of thifluzamide Figure 3 A shows the effect on fresh weight of aboveground parts of rice, Figure 3 B shows the effect on accumulation of thifluzamide in rice. Different lowercase letters in the figure represent significant differences among different tyrosine addition amounts at P<0.05.

[0024] Figure 4 Effect of different biostimulants and combinations on accumulation of three pesticides in aboveground parts of rice Figure 4 A shows the effect on accumulation of thifluzamide in aboveground parts of rice; Figure 4 B shows the effect on accumulation of azoxystrobin in aboveground parts of rice; Figure 4 C shows the effect on accumulation of difenoconazole in aboveground parts of rice. Different lowercase letters in the figure represent significant differences among different biostimulant treatment groups at P<0.05.

[0025] Figure 5 Disease index of rice at 60 d of growth in different treatment groups. Among them, A is the control group without thifluzamide and biostimulant; B is the 100% thifluzamide treatment; C is the 100% thifluzamide + tyrosine + thymol treatment; D is the 75% thifluzamide + tyrosine + thymol treatment; E is the 50% thifluzamide + tyrosine + thymol treatment. Different lowercase letters in the figure represent significant differences among different treatment groups at P<0.05.

[0026] Figure 6 Accumulation amount of thifluzamide in aboveground parts of rice in different treatment groups. B is the 100% thifluzamide treatment; C is the 100% thifluzamide + tyrosine + thymol treatment; D is the 75% thifluzamide + tyrosine + thymol treatment; E is the 50% thifluzamide + tyrosine + thymol treatment. Different lowercase letters in the figure represent significant differences among different treatment groups at P<0.05. Specific implementation manners

[0027] The tested rice variety used in the following examples: Nanjing 5055, with medium susceptibility to sheath blight.

[0028] The tested pesticides used in the following examples: thifluzamide suspension concentrate (240 g / L, Jiangsu Bangsheng Biotechnology Co., Ltd.); difenoconazole suspension concentrate (10 wt%, Guangdong Planton Biotechnology Co., Ltd.); azoxystrobin suspension concentrate (250 g / L, Shandong Weifang Double Star Pesticide Co., Ltd.).

[0029] Other reagents used in the following examples: 20 kinds of amino acids (glycine, alanine, valine, leucine, isoleucine, methionine, proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, aspartic acid, glutamic acid, lysine, arginine, histidine) were all purchased from Shanghai Yuanye Bio-Technology Co., Ltd.; acetonitrile (chromatographic grade, from Merck, Germany); ethylenediamine-N-propylsilane (PSA, 40 - 60 μm, from Tianjin Bonna-Agela Technologies Co., Ltd.), thymol (AR, 98%) was purchased from Shanghai Macklin Biochemical Co., Ltd., and the staining agent (from Qingdao Runhua Agricultural Technology Co., Ltd.). Deionized water used in the experiment was ultrapure water (18.2 MΩcm -1 ).

[0030] In the following examples, the methods for determining the contents of thifluzamide, difenoconazole, and azoxystrobin: After weighing the rice tissues, transfer them into a 10 mL plastic centrifuge tube, add 4 steel balls with a diameter of 7 mm, grind them under liquid nitrogen freezing, add 4 mL of acetonitrile, place them in a multi-tube vortex mixer and vortex (vortex 2 times at 2000 r / min, intermittent vortex mode, interval 1 s, 5 min each time), take 1 mL of the upper organic phase, add 25 mg of PSA and 0.5 g of sodium chloride, vortex for 1 min, and then filter through a 0.22 μm organic filter membrane for testing. Thifluzamide, difenoconazole, and azoxystrobin were all detected by LC-MS / MS method. The chromatographic conditions of LC-MS / MS were as follows: Luna Omega PolarC18 chromatographic column (2.1 mm × 100 mm × 1.6 μm, from Phenomenex, USA); mobile phase A was 0.1% formic acid solution, and B was acetonitrile. The isocratic elution program was from 0 to 2 min, and the volume ratio of the mobile phase was 20% A: 80% B. The column temperature was 25 °C, the flow rate was 0.3 mL / min, and the injection volume was 2.0 μL. The mass spectrometry conditions were as follows: electrospray ESI+ ion source, MRM scan analysis, the ion source temperature was 500 °C, the electrospray voltage was -4500 V, GS1 was set to 50, GS2 was set to 50, CUR was set to 40, and CAD was set to 9. For thifluzamide, negative ion mode; qualitative ions 527.6 / 359.6, collision energy -24 eV; quantitative ions 527.6 / 165.9, collision energy -29 eV. For difenoconazole, positive ion mode; qualitative ions 406.1 / 251, collision energy +35 eV; quantitative ions 406.1 / 337, collision energy +24 eV. For azoxystrobin, positive ion mode; qualitative ions 404.1 / 372, collision energy +20 eV; quantitative ions 404.1 / 344, collision energy +34 eV.

[0031] Example 1 Amino Acid Screening and Optimization of Seed Dressing Rate

[0032] 1. Screen the types of amino acids that promote rice growth

[0033] After disinfecting rice seeds with sodium hypochlorite, soak them in deionized water for 24 h and then drain the water. Weigh 120 g of wet seeds (dry weight is about 100 g), and add amino acids for seed dressing treatment (a total of 21 treatment groups, including a control group without amino acids and 20 treatment groups with single amino acid seed dressing). The concentration of the 20 amino acid mother liquors is 100 mmol / L, and the addition amount is 2 mL / 100 g dry seeds. Add 400 μL of staining agent to each treatment. Shake the rice seeds evenly at 600 r / min for 10 min, and then spread them out to dry naturally until there is no obvious moisture on the surface. Transfer the seeds into a special rice seedling raising substrate, with 9 seeds per hole and 6 holes for each treatment. Cover the seeds with a thin layer of soil, and regularly spray deionized water on the soil surface to keep the soil moist. After 14 d, harvest the aboveground parts of the rice, and measure the fresh weight and plant height of the aboveground parts.

[0034] The experimental results show that compared with the control group without amino acids, there is no significant change in the plant height of rice after treatment with 20 amino acids ( Figure 1 A). Nine amino acids, namely serine (Ser), tyrosine (Tyr), asparagine (Asn), glutamine (Gln), glutamate (Glu), arginine (Arg), lysine (Lys), cysteine (Cys), and aspartic acid (Asp), have a promoting effect on the fresh weight of the aboveground parts of rice. Among them, tyrosine has the most significant promoting effect on the fresh weight of the aboveground parts, and the promoting effects of the other 8 amino acids are not significant ( Figure 1 B). Six amino acids (serine, tyrosine, asparagine, glutamine, glutamate, arginine) were selected in this invention for further research.

[0035] 2. Screening of amino acids that promote the absorption effect of pesticides

[0036] Soak, dress the seeds of rice (add 2.08 g of thifluzamide suspension, 400 μL of staining agent and 2 mL of 6 single amino acid solutions with a concentration of 100 mmol / L to 100 g of dry seeds), dry them naturally, plant them in a special rice seedling raising plug tray. After culturing for 14 d, harvest the aboveground parts of the rice. There are 5 parallels for each treatment, and 4 seedlings are harvested from each parallel sample. Weigh the fresh weight of the aboveground parts of the seedlings. After the samples are ground with liquid nitrogen and extracted with acetonitrile, detect the residue of thifluzamide by LC-MS, and preferably select the type of amino acid with the best absorption-promoting function.

[0037] The experimental results are as Figure 2 shown. Arginine (Arg), asparagine (Asn) and tyrosine (Tyr) have a promoting effect on the fresh weight of the aboveground parts of rice ( Figure 2 A), and the promoting effects of other amino acids are not obvious; arginine (Arg), glutamine (Gln), serine (Ser) and tyrosine (Arg) have a promoting effect on the accumulation of thifluzamide in the aboveground parts of rice ( Figure 2B), in which the promoting effects are significant after seed dressing with serine and tyrosine, and the accumulation amounts of thifluzamide are increased by 26.9% and 43.3% respectively. Since tyrosine has the strongest promoting effect on the absorption of thifluzamide, tyrosine is selected for the next step of research.

[0038] 3. Optimization of the seed dressing amount of tyrosine

[0039] On the basis of the experiment "2. Screening of amino acids promoting the absorption effect of pesticides" in this example, the addition amount of tyrosine is further optimized through a seed dressing experiment. The seed dressing method is the same as above, in which the concentration of the tyrosine mother liquor is 100 mmol / L, and the seed dressing amounts are 0.074, 0.147, 0.294, 0.441 and 0.588 mg / g dry seeds.

[0040] The results show that when the addition amount of tyrosine is 0.074 - 0.147 and 0.441 - 0.588 mg / g dry seeds, there is no significant difference in the fresh weight of the above-ground parts of rice compared with the control group without tyrosine. When the addition amount of tyrosine is 0.294 mg / g dry seeds, the fresh weight of the above-ground parts of rice increases significantly ( Figure 3 A); when different amounts of tyrosine are added for seed dressing, the accumulation amount of thifluzamide in the above-ground parts of rice shows a trend of first increasing and then decreasing. When the addition amount of tyrosine is 0.294 - 0.441 mg / g dry seeds, the accumulation amount of thifluzamide is significantly higher than that of the control group, and the promotion ratio is 24.1% - 25.0% ( Figure 3 B). The optimal seed dressing amount of tyrosine is 0.294 mg / g dry seeds.

[0041] In this example, rice is used as the research object and thifluzamide is used as the test pesticide. The types of amino acids that promote the growth of rice are selected from 20 kinds of amino acids, and the type of amino acid with the best promoting effect on pesticide absorption - tyrosine is further screened out, and the optimal seed dressing amount of tyrosine is selected.

[0042] Example 2 Optimization of the biostimulant formula and evaluation of the promoting effects on different control agents

[0043] The biostimulant described in this example includes tyrosine and / or thymol. After disinfecting, soaking and draining the rice seeds, 3 kinds of pesticides are added according to the recommended seed dressing dose (2.08 g of thifluzamide suspension, 100 μL of difenoconazole suspension or 60 μL of azoxystrobin suspension are added to 100 g of dry seeds). Three groups of biostimulant treatments are set, namely:

[0044] Tyrosine treatment group: The seed dressing amount of tyrosine is 0.294 mg / g dry seeds;

[0045] Thymol treatment group: The seed dressing amount of thymol is 0.3 mg / g dry seeds;

[0046] Tyrosine + thymol treatment group: The total amount of tyrosine and thymol for seed dressing is 0.594 mg / g dry seeds, among which the amount of tyrosine for seed dressing is 0.294 mg / g dry seeds, and the amount of thymol for seed dressing is 0.3 mg / g dry seeds;

[0047] Control group (CK): No biostimulant is added.

[0048] The dressed seeds were transferred into a special rice seedling raising substrate, with 9 seeds per hole, 6 holes for each treatment, covered with a thin layer of soil, and deionized water was regularly sprayed on the soil surface to keep the soil moist. After 14 days, the above-ground parts of the rice were harvested, and the contents of 3 pesticides in the above-ground parts of the rice were measured.

[0049] The experimental results showed that, as Figure 4 shown, tyrosine, thymol, and the tyrosine + thymol treatment group all had a promoting effect on the absorption of the 3 pesticides. The specific results are as follows:

[0050] (1) Thifluzamide: Compared with the control group, after treatment with tyrosine, the accumulation amount of thifluzamide in the above-ground parts of the rice increased by 17.9%; after treatment with thymol, the accumulation amount of thifluzamide in the above-ground parts of the rice increased by 23.7%; after treatment with tyrosine + thymol, the accumulation amount of thifluzamide in the above-ground parts of the rice increased by 32.2% ( Figure 4 A).

[0051] (2) Azoxystrobin: Compared with the control group, after treatment with tyrosine, the accumulation amount of azoxystrobin in the above-ground parts of the rice increased by 7.7%; after treatment with thymol, the accumulation amount of azoxystrobin in the above-ground parts of the rice increased by 29.0%; after treatment with tyrosine + thymol, the accumulation amount of azoxystrobin in the above-ground parts of the rice increased by 38.2% ( Figure 4 B).

[0052] (3) Difenoconazole: Compared with the control group, after treatment with tyrosine, the accumulation amount of difenoconazole in the above-ground parts of the rice increased by 10.4%; after treatment with thymol, the accumulation amount of difenoconazole in the above-ground parts of the rice increased by 23.9%; after treatment with tyrosine + thymol, the accumulation amount of difenoconazole in the above-ground parts of the rice increased by 49.4% ( Figure 4 C).

[0053] In summary, it was found that the combined treatment of tyrosine + thymol had a better promoting effect on the absorption of the 3 pesticides than the individual treatment of tyrosine and thymol. Therefore, the preferred biostimulant formulation is the tyrosine + thymol treatment.

[0054] In this example, three pesticides (thifluzamide, azoxystrobin, and difenoconazole) were used as test agents to study the differences in pesticide accumulation in the target parts of rice (the above-ground parts, where sheath blight mainly infects the above-ground stems and leaves) under the conditions of dressing seeds with tyrosine, thymol, and the combination of tyrosine + thymol respectively, to clarify the promoting effects of the three stimulants on the absorption of pesticides by rice, and it was found that the combination of tyrosine + thymol had a better effect than single-agent seed dressing and had an absorption-promoting function for the main agents for controlling sheath blight.

[0055] Example 3 Evaluation of the control effect of biostimulant formulations on sheath blight and their application effects in reducing pesticide use and increasing efficiency

[0056] After disinfecting, soaking, and drying the rice seeds, seed dressing treatments were carried out according to the following five different treatment methods A - E. Five treatment groups were set up, namely:

[0057] Group A: The control group without thifluzamide and biostimulant;

[0058] Group B: The treatment group with 1-fold recommended dose (100%) of thifluzamide;

[0059] Group C: The treatment group with 1-fold recommended dose (100%) of thifluzamide + biostimulant;

[0060] Group D: The treatment group with 0.75-fold recommended dose (75%) of thifluzamide + biostimulant;

[0061] Group E: The treatment group with 0.5-fold recommended dose (50%) of thifluzamide + biostimulant;

[0062] Among them, the 1-fold recommended dosage of thifluzamide was to add 2.08 g of thifluzamide suspension to 100 g of dry seeds; the biostimulant treatment was the combination of tyrosine + thymol, and the seed dressing amounts of tyrosine and thymol in the combination were 0.294 and 0.3 mg / g of dry seeds respectively. The dressed seeds were transferred into a special rice seedling raising substrate, with 9 seeds per hole, 12 holes for each treatment, covered with a thin layer of soil, and deionized water was regularly sprayed on the soil surface to keep the soil moist. After the rice seedlings emerged, they were transplanted into black plastic buckets (size: upper diameter 21.4 cm, lower diameter 19.1 cm, bucket mouth diameter 20.7 cm), with 4 parallels (small buckets) for each treatment, 2 kg of paddy soil was added to each small bucket, 3 holes per bucket, and 3 rice seedlings per hole. When the rice grew to 45 d, the pathogenic bacteria of sheath blight (Rhizoctonia solania) were inoculated at the leaf sheath position of the rice, and when it grew to 60 d, the above-ground parts of the rice were harvested, the thifluzamide content in the above-ground parts of the rice was measured, and the disease index of each treatment group was calculated.

[0063] Method for inoculating Rhizoctonia solani: Inoculate Rhizoctonia solani onto PDA medium, and activate and culture it in an inverted position at 28 °C in an incubator for 2 - 3 days. Take it out after the surface of the medium is covered with white mycelia. Inoculate the activated bacterial blocks into PDA medium, and culture them in an inverted position at 28 °C for 3 days. Take them out for standby after the white mycelia cover the culture dish. Use a punch to take bacterial blocks with a diameter of 7 mm at the same radius of the culture dish, and place the bacterial blocks at the base of the rice seedlings with the mycelial surface attached to the base of the rice stem.

[0064] The described method for calculating disease index: Investigate the disease level using the "leaf sheath position method". Randomly select 4 seedlings for each treatment, cut off the roots of the rice, and use a steel ruler to measure the height of the rice seedlings (the height from the base of the stem to the highest leaf tip after the rice is straightened) and the lesion height of the diseased plants. The formula for calculating the disease level of a single plant is (lesion height / seedling height) × 9, and the relative disease level (disease index) is the average value of the disease levels of 16 single plants.

[0065] The results are as follows:

[0066] (1) In terms of the rice phenotype, the control group (Group A) without pesticides and biostimulants had severe disease, mainly manifested as the formation of cloud-like lesions on the rice stems and leaves, and partial wilting of the stems and leaves; the disease incidence in the groups treated with thifluzamide was relatively light (Groups B - E), and among them, the growth of rice in the groups treated with biostimulants (Groups C - E) was significantly better than that in the group without biostimulants (Group B).

[0067] (2) In terms of the disease index, the control group (Group A) without pesticides and biostimulants had the most severe disease Figure 5 ), the group with 100% thifluzamide + biostimulant (Group C) had the lightest disease incidence, and there was no significant difference in the disease incidence among the other three treatment groups (Groups B, D, E) Figure 5 ).

[0068] (3) In terms of the thifluzamide content, when the dosage of thifluzamide was 1 times the recommended dosage, compared with the control (Group B), the thifluzamide content in the above-ground part of the rice in the treatment group with biostimulant added (Group C) increased significantly, and the increase ratio reached 63.2% Figure 6 ); when the dosage of thifluzamide was 75% and combined with biostimulant seed dressing treatment (Group D), the thifluzamide content in the above-ground part of the rice was still significantly higher than that in the control group (Group B) ( Figure 6 ); when the dosage of thifluzamide was 50% and combined with biostimulant seed dressing treatment (Group E), the thifluzamide content in the above-ground part of the rice was equivalent to that in the control group (Group B) ( Figure 6 ), indicating that the biostimulant combination used in the present invention has an obvious promoting effect on the absorption of thifluzamide by rice.

[0069] The present invention provides an idea and method of a biostimulant and its application in promoting the absorption of pesticides by crops. There are many methods and ways to specifically implement this technical solution. The above description is only the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention. Each component not clearly defined in this embodiment can be implemented by existing technologies.

Claims

1. A use of a biostimulant in promoting the absorption of pesticides by crops, wherein the biostimulant comprises thymol.

2. The use according to claim 1, characterized in that: The crop is rice.

3. The use according to claim 1, characterized in that: The pesticide is a drug for preventing and controlling sheath blight; the drug for preventing and controlling sheath blight includes any one or more combinations of thiophanate-methyl, myclobutanil or difenoconazole.

4. The use according to claim 1, characterized in that: The biostimulant includes amino acids.

5. The use according to claim 4, characterized in that: The amino acid is tyrosine.

6. The use according to claim 4, characterized in that: The mass ratio of thymol to amino acid in the biostimulant is 1:5 to 5:

1.

7. The use according to any one of claims 1 to 6, characterized in that: The method for promoting the absorption of pesticides by crops by using the biostimulant is as follows: after mixing the biostimulant with the pesticide, the crop seeds are mixed with the biostimulant to promote the absorption of the pesticides by the crops.

8. The use according to claim 7, characterized in that: The dosage of thymol is 0.07-0.4 mg / g dry seeds.

9. A biostimulant for promoting the absorption of pesticides by crops, characterized in that: The biostimulant comprises thymol and amino acid.

10. The biostimulant according to claim 9, characterized in that: The amino acid is tyrosine.

Images