A fermented biological feed of white spirit lees and a preparation method thereof

Abstract

The application discloses a kind of fermented biological feed of white spirit vinasse and a preparation method thereof, belong to the technical field of feed.The fermented biological feed of white spirit vinasse is uniformly mixed and fermented by the following weight parts of raw materials: white spirit vinasse: 60-80 parts, corn flour: 10-20 parts, wheat bran: 5-15 parts, calcium hydrogen phosphate: 0.5-2 parts, enzyme preparation: 0.5-2 parts, shaping agent: 0.2-0.5 parts, preservative: 0.2-0.5 parts;Among them, the enzyme preparation is composed of yeast, bacillus subtilis and lactobacillus plantarum;The shaping agent is modified guaiacyl lignin obtained by the reaction of guaiacyl lignin and 5-aminovaleric acid, and then reacts with ethylenediamine to obtain.The fermented biological feed of white spirit vinasse prepared by the application has excellent preservative effect and good powder rate, which is beneficial to the storage and transportation of feed.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of feed, in particular to a fermented biological feed of distiller's grains and a preparation method thereof. BACKGROUND

[0002] The annual output of Chinese liquor is about several million tons, and distiller's grains, as a byproduct of Chinese liquor, is a good feed, which is beneficial to the growth of livestock and poultry. Distiller's grains is rich in nutrients, including protein, cellulose, and various vitamins and minerals, and can provide comprehensive nutrition required for the growth of livestock and poultry, helping to improve the immunity and growth rate of livestock and poultry, and promote healthy development. However, distiller's grains contains about 50% of rice hull which is difficult to digest, and directly feeding livestock and poultry will affect the digestion and absorption of animals. Fresh distiller's grains is high in moisture and acidity, and is prone to rot and deterioration, which is not easy to store and transport. A large amount of distiller's grains is randomly stacked, which seriously pollutes the environment.

[0003] Pelleted feed has been widely used in various breeding fields. Production practice in recent years has proved that, compared with powder, pelleted feed has good palatability and can prevent livestock and poultry from being picky eaters. However, the pelleted feed is prone to breakage, wear, and pulverization during long-distance transportation and handling.

[0004] A fermentation method for preparing distiller's grains biological feed is disclosed in Chinese patent application No. CN118203070A. In this invention, water is added to the composite microbial agent and dry material, and then fermented for 2 days. After adding a preservative aid, the distiller's grains biological feed is obtained after drying. The crude protein content is increased, and the crude fiber content is reduced, which helps to improve the bioavailability of nutrients and reduce the anti-nutritional factors in distiller's grains. However, the powder is not conducive to transportation and storage. SUMMARY

[0005] In view of the deficiencies of the prior art, the present application aims to provide a fermented biological feed of distiller's grains and a preparation method thereof.

[0006] To achieve the above-mentioned purpose, the present application realizes the following technical solutions:

[0007] A fermented biological feed of distiller's grains is prepared by uniformly mixing the following raw materials in parts by weight:

[0008] distiller's grains: 60-80 parts, corn flour: 10-20 parts, wheat bran: 5-15 parts, calcium hydrogen phosphate: 0.5-2 parts, enzyme preparation: 0.5-2 parts, shaping agent: 0.2-0.5 parts, preservative: 0.2-0.5 parts;

[0009] The enzyme preparation is composed of yeast, Bacillus subtilis, and Lactobacillus plantarum in a mass ratio of 2:(0.8-1.2):(0.8-1.3);

[0010] The sizing agent is prepared by the following method:

[0011] The sizing agent is prepared by the following method:

[0012] S1: guaiacyl lignin reacts with 5-aminovaleric acid under the action of formaldehyde aqueous solution to obtain modified guaiacyl lignin; the reaction equation is as follows:

[0013]

[0014] S2: the modified guaiacyl lignin reacts with ethylenediamine under the action of N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to obtain the sizing agent; the reaction equation is as follows:

[0015]

[0016] It should be noted that the reaction equation only represents the reaction between the functional groups of molecules.

[0017] The feeding mass ratio of the guaiacyl lignin and 5-aminovaleric acid in step S1 is 10:(2-3).

[0018] The feeding mass ratio of the modified guaiacyl lignin, N-hydroxysuccinimide, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and ethylenediamine in step S2 is 40:(10-15):(15-24):(3-5).

[0019] The preservative is prepared by the following method:

[0020] N1: Michael addition reaction occurs between the amino group of carboxymethyl chitosan and the carbon-carbon double bond of methacrylpropyltrimethylammonium chloride to generate a quaternary ammonium salt compound; the reaction equation is as follows:

[0021]

[0022] In the reaction equation, m=t+s.

[0023] The feeding mass ratio of the carboxymethyl chitosan and methacrylpropyltrimethylammonium chloride is 2.5:1.

[0024] N2: Schiff base reaction occurs between the amino group in the quaternary ammonium salt compound and the aldehyde group in 2-nitro-4-methoxybenzaldehyde to generate the preservative; the reaction equation is as follows:

[0025]

[0026] In the reaction equation, s=o+r.

[0027] The mass ratio of the quaternary ammonium salt compound to 2-nitro-4-methoxybenzaldehyde is 5:3.

[0028] A preparation method of a fermented biological feed of white spirit lees, comprising the following steps:

[0029] (1) The following ingredients are weighed by weight parts: white spirit lees: 60-80 parts, corn flour: 10-20 parts, wheat bran: 5-15 parts, calcium hydrogen phosphate: 0.5-2 parts, enzyme preparation: 0.5-2 parts, shaping agent: 0.2-0.5 parts, and preservative: 0.2-0.5 parts;

[0030] (2) The white spirit lees, corn flour, wheat bran, calcium hydrogen phosphate and enzyme preparation are stirred and mixed, water is added, the humidity is adjusted to 40-60%, and fermentation is carried out at 20-40℃ for 3 days to obtain a fermentation system;

[0031] (3) The shaping agent and the preservative are added to the fermentation system, stirred uniformly, and pressed and shaped to obtain the fermented biological feed of white spirit lees.

[0032] Due to the above technical scheme, the present application has the following beneficial effects:

[0033] (1) The shaping agent prepared in the present application is based on guaiacyl lignin, which is treated by carboxylation and reacts with ethylenediamine to form a network macromolecule, thereby enhancing the stability of the feed particles and reducing the pulverization rate. The shaping agent contains a large amount of phenolic hydroxyl groups, ether bonds and amide bonds, which can enhance the adhesion performance through hydrogen bonding, thereby further reducing the pulverization rate. The phenolic hydroxyl groups of guaiacyl lignin are connected to methoxy groups and -CH2-NH- groups at the ortho position, respectively, and the electronic effect thereof can promote the release of hydrogen ions, capture free radicals and slow down the oxidation and deterioration of the feed.

[0034] (2) The preservative in the present application can destroy the cell membrane of microorganisms, interfere with the synthesis of DNA and proteins, and cause cell death through the synergistic effect of amino groups and quaternary ammonium salts. The nitro group and the Schiff base can interfere with the metabolic process of bacteria, hinder the growth and reproduction, and improve the preservative effect by metabolizing products and inhibiting enzyme activity. DETAILED DESCRIPTION

[0035] The present application will be further described below in conjunction with examples, but the present application is not limited to these examples.

[0036] Example 1 Preparation of a shaping agent

[0037] S1 : Under nitrogen protection, 500 g of deionized water, 90 g of 37 wt% formaldehyde aqueous solution, 20 g of sodium hydroxide and 20 g of 5-aminovaleric acid were sequentially added into a reaction kettle, stirred and mixed uniformly, 100 g of guaiacyl lignin was added, heated to 60°C, reacted for 10 h, cooled to room temperature, the solid was precipitated by adjusting the pH to 7 using 1M HC1 solution, filtered, washed with deionized water (300 ml x 2 times), vacuum dried at 80°C for 10 h to obtain modified guaiacyl lignin;

[0038] S2: Under ice bath, 500 ml of dimethyl sulfoxide, 40 g of modified guaiacyl lignin were added into a reaction kettle, stirred and mixed uniformly, 10 g of N-hydroxysuccinimide and 15 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were added and stirred for 30 min, 3 g of ethylenediamine was added, heated to 30°C, stirred and reacted for 6 h, cooled to room temperature, filtered, washed with deionized water (300 ml x 2 times), vacuum dried at 70°C for 24 h to obtain a sizing agent.

[0039] Example 2 Preparation of sizing agent

[0040] S1 : Under nitrogen protection, 500 g of deionized water, 80 g of 37 wt% formaldehyde aqueous solution, 20 g of sodium hydroxide and 25 g of 5-aminovaleric acid were sequentially added into a reaction kettle, stirred and mixed uniformly, 100 g of guaiacyl lignin was added, heated to 70°C, reacted for 8 h, cooled to room temperature, the solid was precipitated by adjusting the pH to 7 using 1M HC1 solution, filtered, washed with deionized water (300 ml x 2 times), vacuum dried at 80°C for 10 h to obtain modified guaiacyl lignin;

[0041] S2: Under ice bath, 500 ml of dimethyl sulfoxide, 40 g of modified guaiacyl lignin were added into a reaction kettle, stirred and mixed uniformly, 10 g of N-hydroxysuccinimide and 15 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were added and stirred for 30 min, 3 g of ethylenediamine was added, heated to 30°C, stirred and reacted for 6 h, cooled to room temperature, filtered, washed with deionized water (300 ml x 2 times), vacuum dried at 70°C for 24 h to obtain a sizing agent.

[0042] Example 3 Preparation of sizing agent

[0043] S1 : Under nitrogen protection, 500 g of deionized water, 80 g of 37 wt% formaldehyde aqueous solution, 20 g of sodium hydroxide and 25 g of 5-aminovaleric acid were sequentially added into a reaction kettle, stirred and mixed uniformly, 100 g of guaiacyl lignin was added, heated to 70°C, reacted for 8 h, cooled to room temperature, the solid was precipitated by adjusting the pH to 7 using 1M HC1 solution, filtered, washed with deionized water (300 ml x 2 times), vacuum dried at 80°C for 10 h to obtain modified guaiacyl lignin;

[0044] S2: 500 ml dimethyl sulfoxide, 40 g modified guaiacyl lignin were added into the reaction kettle under ice bath, stirred and mixed, 15 g N-hydroxysuccinimide and 24 g 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were added and stirred for 2 h, 5 g ethylenediamine was added, the temperature was raised to 40 °C, and the reaction was carried out for 4 h, then the temperature was cooled to room temperature, filtered, washed with deionized water (300 ml x 2 times), and vacuum dried at 70 °C for 24 h to obtain a shaping agent.

[0045] Example 4 Preparation of preservative

[0046] N1: 1000 g water, 50 g carboxymethyl chitosan were added into the reaction bottle, stirred at 40 °C for 2 h, the pH was adjusted to 9 using 5 wt% sodium hydroxide solution, 20 g methyl acryloyl propyl trimethyl ammonium chloride was added under nitrogen protection, and the reaction was carried out at 40 °C for 18 h (during which the pH stability was monitored, and the pH was adjusted to be in the range of 8-9 using 5 wt% sodium hydroxide solution), 1M HCl was added dropwise to adjust the pH to 7.0, 3000 g anhydrous ethanol was added to precipitate the solid, filtered, washed with 80 wt% ethanol for 3 times (100 ml each time), and vacuum dried at 40 °C for 24 h to obtain a quaternary ammonium salt compound;

[0047] N2: 25 g acetic acid, 400 g water, and 50 g quaternary ammonium salt compound were sequentially added into the reaction bottle, stirred and mixed for 2 h, 180 g 2-nitro-4-methoxybenzaldehyde methanol solution (containing 30 g 2-nitro-4-methoxybenzaldehyde) was added, stirred at room temperature for 24 h, 400 g acetone was added to stir and precipitate the solid, filtered, washed with 5 wt% sodium bicarbonate aqueous solution twice (200 g each time), washed with 200 g methanol once, and vacuum dried at 50 °C for 10 h to obtain a preservative.

[0048] Example 5 Preparation of fermented bio-feed of distiller's grains

[0049] (1) Take: distiller's grains 600 g, corn flour 100 g, wheat bran 50 g, calcium hydrogen phosphate 5 g, enzyme preparation 5 g, shaping agent (prepared in example 1) 2 g, preservative (prepared in example 4) 2 g;

[0050] Among them, the enzyme preparation is composed of 2.8 g of yeast, 1.1 g of bacillus subtilis, and 1.1 g of lactobacillus;

[0051] (2) The distiller's grains, corn flour, wheat bran, calcium hydrogen phosphate, and enzyme preparation were stirred and mixed, water was added to adjust the humidity to 40%, and the fermentation system was obtained after fermentation at 20 °C for 3 days;

[0052] (3) The shaping agent and the preservative were added into the above fermentation system, stirred uniformly, dried to a water content of 20%, and then pressed and shaped to obtain the fermented bio-feed of distiller's grains.

[0053] Example 6 Preparation of fermented bio-feed of distiller's grains

[0054] (1) Take: distiller's grains 700g, corn flour 180g, wheat bran 120g, calcium hydrogen phosphate 10g, enzyme preparation 10g, shaping agent (prepared in Example 2) 4g, preservative (prepared in Example 4) 4g;

[0055] Among them, the enzyme preparation is composed of yeast 5g, bacillus subtilis 2.5g, lactobacillus 2.5g;

[0056] (2) Mix the distiller's grains, corn flour, wheat bran, calcium hydrogen phosphate, enzyme preparation by stirring, add water, adjust the humidity to 50%, and ferment at 35℃ for 3 days to obtain a fermentation system;

[0057] (3) Add the shaping agent and preservative to the above fermentation system, stir uniformly, dry to a water content of 20%, and press to shape to obtain the fermented bio-feed of distiller's grains.

[0058] Example 7 Preparation of fermented bio-feed of distiller's grains

[0059] (1) Take: distiller's grains 800g, corn flour 200g, wheat bran 150g, calcium hydrogen phosphate 20g, enzyme preparation 20g, shaping agent (prepared in Example 3) 5g, preservative (prepared in Example 4) 5g;

[0060] Among them, the enzyme preparation is composed of yeast 8.9g, bacillus subtilis 5.3g, lactobacillus 5.8g;

[0061] (2) Mix the distiller's grains, corn flour, wheat bran, calcium hydrogen phosphate, enzyme preparation by stirring, add water, adjust the humidity to 60%, and ferment at 40℃ for 3 days to obtain a fermentation system;

[0062] (3) Add the shaping agent and preservative to the above fermentation system, stir uniformly, dry to a water content of 20%, and press to shape to obtain the fermented bio-feed of distiller's grains.

[0063] Comparative Example 1

[0064] The preparation method of the fermented bio-feed of distiller's grains is basically the same as that of Example 6, except that the shaping agent (prepared in Example 2) is replaced with equal mass of guaiacyl lignin.

[0065] Comparative Example 2

[0066] The preparation method of the fermented bio-feed of distiller's grains is basically the same as that of Example 6, except that the shaping agent (prepared in Example 2) is replaced with equal mass of the shaping agent prepared by the following method:

[0067] The preparation method of the shaping agent is basically the same as that in Example 2, except that the ethylenediamine in step S2 is replaced by propylamine of equal weight.

[0068] Comparative Example 3

[0069] The preparation method of the fermented bio-feed of distiller's grains is basically the same as that in Example 6, except that the shaping agent (prepared in Example 2) is replaced by a shaping agent prepared by the following method:

[0070] Under nitrogen protection, 500 g of deionized water, 80 g of 37 wt% formaldehyde aqueous solution, 20 g of sodium hydroxide and 10 g of ethylenediamine were sequentially added into a reaction kettle, stirred and mixed, 100 g of guaiacyl lignin was added, the temperature was raised to 70°C, and the reaction was carried out for 8 h. After cooling to room temperature, 1M HCl solution was used to adjust the pH to 7 to precipitate the solid, which was filtered, washed with deionized water (300 ml x 2 times), and vacuum dried at 80°C for 10 h to obtain the shaping agent.

[0071] Comparative Example 4

[0072] The preparation method of the fermented bio-feed of distiller's grains is basically the same as that in Example 6, except that the preservative (prepared in Example 4) is replaced by carboxymethyl chitosan of equal weight.

[0073] Comparative Example 5

[0074] The preparation method of the fermented bio-feed of distiller's grains is basically the same as that in Example 6, except that the preservative (prepared in Example 4) is replaced by a preservative prepared by the following method:

[0075] The preparation method of the preservative is basically the same as that in Example 4, except that the methacrylpropyltrimethylammonium chloride in step N1 is replaced by N-(3-dimethylaminopropyl) methacrylamide of equal weight.

[0076] Comparative Example 6

[0077] The preparation method of the fermented bio-feed of distiller's grains is basically the same as that in Example 6, except that the preservative (prepared in Example 4) is replaced by a preservative prepared by the following method:

[0078] The preparation method of the preservative is basically the same as that in Example 4, except that the 2-nitro-4-methoxybenzaldehyde in step N2 is replaced by 3-methoxy-4-hydroxybenzaldehyde of equal weight.

[0079] The distiller's grains used in the examples and comparative examples of the present application are produced by Bozhou Gujinggong Liquor Co., Ltd., and are the residues left after brewing of wheat, corn, LiaoXi highland barley, rice and waxy rice; the yeast (2 x 10 10 CFU / g), Bacillus subtilis (5 x 1010 Lactobacillus plantarum (1x10 10 The carboxymethyl chitosan has a number average molecular weight of 10K.

[0080] The white spirit lees fermented biological feed prepared in Examples 5-7 and Comparative Examples 1-6 was subjected to a mildew-proof performance test, and the test method was as follows:

[0081] The white spirit lees fermented biological feed prepared in Examples and Comparative Examples was placed into 5 piles of equal amount, each pile weighing 300g, for a total of 45 piles, and was placed in a constant temperature and humidity ventilated light-proof condition at a temperature of 35℃ and a humidity of 80%. The white spirit lees biological feed in each pile was subjected to sensory evaluation at 1d, 4d, 6d, 10d and 15d, and the evaluation was made using a 5-point system. The specific evaluation criteria were as follows, and the scores of each group of Examples / Comparative Examples were averaged over the five piles.

[0082] 5 points: no change in feed color, fermentation aroma, no mold spots and mycelium observed by naked eye;

[0083] 4 points: slight change in feed color, fermentation aroma, very few mold spots observed by naked eye, and no mycelium;

[0084] 3 points: slight change in feed color, weak sour smell, a small amount of mold spots observed by naked eye, and no mycelium;

[0085] 2 points: dark and dull feed color, strong mold smell, a small amount of mold spots and a small amount of mycelium observed by naked eye;

[0086] 1 point: dark and dull feed color, strong rotten mold smell, a large amount of mold spots and a large amount of mycelium observed by naked eye.

[0087] The white spirit lees fermented biological feed prepared in Examples 5-7 and Comparative Examples 1-6 was subjected to a powderization rate test, and the test method was as follows:

[0088] 100g of the white spirit lees fermented biological feed prepared in Examples and Comparative Examples was accurately weighed, placed in a particle durability detector for 60s, and the residual particles were taken out and accurately weighed. The powderization rate was calculated as follows: powderization rate = (100-particle residual amount) / 100x100%; the powderization rate of each sample was determined three times, and the average was taken.

[0089] Table 1 Performance test table

[0090]

[0091] As can be seen from the data of Examples 5, 6 and 7 in Table 1, the white spirit lees fermented biological feed prepared in the present application has excellent mildew-proof performance and good powderization rate, which is beneficial to the storage and transportation of the feed.

[0092] The shaping agent prepared by the present application is based on guaiacyl lignin, which is reacted with ethylenediamine after carboxylation treatment to form a network macromolecule, so that the starch, protein and other feed raw materials can be wrapped and bonded in the feed granulation process, which not only enhances the stability of the feed particles, but also significantly reduces the pulverization rate of the feed during storage and transportation. The shaping agent contains a large number of phenolic hydroxyl groups (-OH), ether bonds (-O-) and amide bonds (-CONH-), which can be combined with the surface of starch, protein and other feed raw materials through hydrogen bonds to enhance the adhesion performance and further reduce the pulverization rate. In addition, the ortho position of the phenolic hydroxyl group of guaiacyl lignin is connected with methoxy (-OCH3) and -CH2-NH- groups, both of which can enhance the electron density of the phenolic hydroxyl group through electron-donating effect, promote the release of H + , and limit the movement of free radical intermediates through steric effect, prolong the reaction time, and enhance the free radical capture effect; the latter can also weaken the O-H bond of the phenolic hydroxyl group through intramolecular hydrogen bond effect, thereby reducing the dissociation energy, promoting the release of H + , and improving the free radical capture efficiency.

[0093] The amino group and the positively charged quaternary ammonium salt in the preservative prepared by the present application synergistically interact with the negatively charged groups on the surface of the microbial cell membrane to destroy the integrity of the cell membrane; they can also penetrate into the cells to interfere with the synthesis of DNA and proteins, leading to the death of microbial cells, thereby achieving the effect of preservation. The nitro group in the preservative can be reduced to active metabolites under the action of bacterial nitroreductase, which can inhibit the DNA metabolism process of bacteria and promote the death of bacteria. The Schiff base formed by the aldehyde group and the amino group can inhibit the activity of enzymes in bacteria, thereby interfering with the metabolic process of bacteria and hindering their growth and reproduction, thereby improving the preservation effect.

[0094] The above is only a preferred embodiment of the present application and is not intended to limit the present application; however, for ordinary skilled persons in the art, some minor changes, modifications and equivalent changes made on the basis of the above disclosed technical content without departing from the scope of the technical solutions of the present application are equivalent embodiments of the present application; at the same time, any equivalent changes, modifications and evolution of the above embodiments made according to the essential technology of the present application are still within the protection scope of the technical solutions of the present application.

Claims

1. A fermented biological feed made from baijiu lees, characterized in that, It is made by mixing and fermenting the following ingredients in the indicated weight proportions: Baijiu lees: 60-80 parts, corn flour: 10-20 parts, wheat bran: 5-15 parts, dicalcium phosphate: 0.5-2 parts, enzyme preparation: 0.5-2 parts, setting agent: 0.2-0.5 parts, preservative: 0.2-0.5 parts; The enzyme preparation is composed of yeast, Bacillus subtilis, and Lactobacillus plantarum. The setting agent is prepared by the following method: S1: Under nitrogen protection, guaiac wood lignin reacts with 5-aminovaleric acid in the presence of formaldehyde aqueous solution to obtain modified guaiac wood lignin. S2: Modified guaiac wood lignin was reacted with ethylenediamine in the presence of N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to prepare a sizing agent.

2. The fermented biological feed made from baijiu lees according to claim 1, characterized in that, The enzyme preparation is composed of yeast, Bacillus subtilis, and Lactobacillus plantarum in a mass ratio of 2:(0.8-1.2):(0.8-1.3).

3. The fermented biological feed made from baijiu lees according to claim 1, characterized in that, The mass ratio of guaiac lignin and 5-aminovaleric acid in step S1 is 10:(2-3).

4. The fermented biological feed made from baijiu lees according to claim 1, characterized in that, The mass ratio of the modified guaiac lignin, N-hydroxysuccinimide, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and ethylenediamine in step S2 is 40:(10-15):(15-24):(3-5).

5. The fermented biological feed made from liquor lees according to claim 1, characterized in that, The preservative is prepared by the following method: N1: Carboxymethyl chitosan reacts with methacryloylpropyltrimethylammonium chloride to form a quaternary ammonium salt compound; N2: Quaternary ammonium salt compounds react with 2-nitro-4-methoxybenzaldehyde to form preservatives.

6. The fermented biological feed made from liquor lees according to claim 5, characterized in that, The mass ratio of carboxymethyl chitosan to methacryloxypropyltrimethylammonium chloride is 2.5:

1.

7. The fermented biological feed made from liquor lees according to claim 5, characterized in that, The mass ratio of the quaternary ammonium salt compound to 2-nitro-4-methoxybenzaldehyde is 5:

3.

8. A method for preparing fermented biological feed from baijiu lees according to any one of claims 1-7, characterized in that, Includes the following steps: (1) Weigh out the following by weight: 60-80 parts of baijiu lees, 10-20 parts of corn flour, 5-15 parts of wheat bran, 0.5-2 parts of dicalcium phosphate, 0.5-2 parts of enzyme preparation, 0.2-0.5 parts of fixative, and 0.2-0.5 parts of preservative; (2) Mix the baijiu lees, corn flour, wheat bran, dicalcium phosphate and enzyme preparation, add water, adjust the humidity to 40-60%, and ferment at 20-40℃ for 3 days to obtain the fermentation system; (3) Add the sizing agent and preservative to the above fermentation system, stir evenly, press and shape to obtain fermented biological feed from liquor lees.

Images