Toothpaste added with regenerated protein composition and preparation method of toothpaste

Abstract

The invention relates to the technical field of toothpaste, and particularly provides toothpaste added with a regenerated protein composition and a preparation method of the toothpaste, the toothpaste comprises the following components in parts by weight: 0.2-10 parts of the regenerated protein composition, 15-70 parts of a humectant, 10-50 parts of a grinding material, 0.5-10 parts of a foaming agent, 0.5-3 parts of a thickening agent and 8-40 parts of water, wherein the regenerated protein composition consists of regenerated protein, a film-forming agent, an adsorbent and polyphosphate. The regenerated protein composition is added into the toothpaste, the adsorbent with an ultra-large specific surface area and a cavity is used for adsorbing bacterial plaque fragments, the cleaning power is improved by combining the chelating and dispersing effects of polyphosphate, the residence of regenerated protein on the surfaces of gingiva and oral mucosa is prolonged by the film-forming agent, anti-inflammatory, hemostasis and tissue regeneration are synergistically promoted, and the toothpaste has the advantages that the toothpaste has the antibacterial and anti-inflammatory effects; the synergistic interaction of cleaning and repairing is realized, and the application prospect is good.

Description

Technical Field

[0001] The present invention relates to the technical field of toothpaste, and in particular to a toothpaste containing a regenerated protein composition and a preparation method thereof. Background Art

[0002] Gingivitis is a common oral disease. The data from the fourth national oral health epidemiological survey shows that the detection rate of gingival bleeding in residents aged 35 - 44 is 87.4%, and the detection rate of dental calculus is 96.7%. This reflects that gingival problems are very common among adults. If gingival problems cannot be effectively controlled and improved, they will further lead to periodontitis. In response to improving gingival problems, many oral care products (mainly toothpaste) containing Chinese herbal medicines or chemical components have emerged in China in the past forty years. However, long-term use of such toothpaste will have some adverse effects on the balance of oral flora and physical health.

[0003] Some existing toothpastes containing functional ingredients have the disadvantages of short residence time of active ingredients and slight irritation or damage to the gums and oral mucosa. In recent years, some toothpastes containing bioactive ingredients have emerged, which have good biocompatibility and safety and play a good role in improving oral health problems.

[0004] Providing a toothpaste that can improve oral health problems has become one of the urgent technical problems to be solved at present. Summary of the Invention

[0005] In view of this, the present invention provides a toothpaste containing a regenerated protein composition and a preparation method thereof.

[0006] The technical solution of the present invention is realized as follows: The present invention provides a toothpaste containing a regenerated protein composition, which comprises the following components in parts by weight:

[0007] Regenerated protein composition: 0.2 - 10 parts

[0008] Humectant: 15 - 70 parts

[0009] Abrasive: 10 - 50 parts

[0010] Foaming agent: 0.5 - 10 parts

[0011] Thickener: 0.5 - 3 parts

[0012] Water: 8 - 40 parts

[0013] The regenerated protein composition is composed of regenerated protein, film-forming agent, adsorbent and polyphosphate.

[0014] In some embodiments, the parts by weight of each component in the regenerated protein composition are:

[0015] Regenerated protein: 0.05 - 1 part

[0016] Film-forming agent: 0.05 - 3 parts

[0017] Adsorbent: 0.05 - 3 parts

[0018] Polyphosphate: 0.05 - 3 parts.

[0019] In some embodiments, the regenerated protein is at least one of fibroin, hyaluronic acid, and hydrolyzed collagen, and the regenerated protein is preferably fibroin and / or hydrolyzed collagen.

[0020] In some embodiments, the film-forming agent is one of carboxymethyl chitosan, polyvinylpyrrolidone, vinyl methyl ether / maleic anhydride copolymer, and poloxamer 407, and the film-forming agent is preferably carboxymethyl chitosan.

[0021] In some embodiments, the adsorbent is one of molecular sieve, diatomaceous earth, and β-cyclodextrin, and the adsorbent is preferably molecular sieve.

[0022] In some embodiments, the polyphosphate is at least one of sodium pyrophosphate, sodium hexametaphosphate, sodium phytate, trisodium phosphate, disodium dihydrogen pyrophosphate, and their potassium salts, and the polyphosphate is preferably sodium pyrophosphate and / or sodium hexametaphosphate.

[0023] Through the ultra-high specific surface area and cavity structure of the adsorbent, combined with the chelating and dispersing effect of polyphosphate, the physical adsorption and chemical cleaning ability of toothpaste for dental plaque and stain debris are significantly improved. The film-forming agent forms a protective film on the oral surface after cleaning, prolongs the residence time of the regenerated protein, and promotes its anti-inflammatory and hemostatic active effects.

[0024] In some embodiments, the weight parts of each component in the regenerated protein composition are:

[0025] Fibroin: 0.05 - 1 part

[0026] Carboxymethyl chitosan: 0.05 - 2 parts

[0027] 4A sodium molecular sieve: 0.05 - 3 parts

[0028] Polyphosphate: 0.05 - 1 part.

[0029] Natural silk is composed of fibroin and sericin. Sericin is wrapped on the outside (about 20-30%), and fibroin is inside (about 70-80%). Fibroin can be hydrolyzed by protease in the body, and the generated amino acids can be absorbed by the human body. For oral injury care, fibroin can help inhibit inflammation, protect the damaged area and promote the repair of damaged mucosa. Fibroin has currently been used in medical dressings to help wound healing. Carboxymethyl chitosan is a kind of chitosan derivative after carboxymethylation reaction of chitosan, and its water solubility and bioavailability are improved. Carboxymethyl chitosan has antibacterial, film-forming, drug-loading effects, and participates in the regeneration of dental and periodontal tissues. It can control gingival inflammation and reduce the symptoms of gingival bleeding in the oral cavity. In toothpaste, it is mainly used for film-forming and assisting antibacterial and anti-inflammatory. In addition to having appropriate friction, the most remarkable feature of molecular sieve is its huge specific surface area and extremely long internal pores, so it has excellent adsorption capacity. When brushing teeth, it can help adsorb and entrap plaque debris and dental stains on the gingival surface and tooth surface, making them clean.

[0030] In order to enhance the biological activity of the regenerated protein, this patent particularly adds a film-forming agent to promote the retention and absorption of the regenerated protein on the oral mucosa and gingival surface.

[0031] In the toothpaste formula added with regenerated protein (fibroin) and film-forming agent (carboxymethyl chitosan), after adding an appropriate amount of polyphosphate and adsorbent, the anti-inflammatory, hemostatic and damage-repairing effects of the toothpaste are significantly improved. It is considered that polyphosphate and molecular sieve may improve the cleaning effect of the toothpaste, making plaque debris and stains detach from the oral cavity and gingival surface, facilitating the film-forming of carboxymethyl chitosan on the oral cavity and gingival surface, and enabling fibroin to play anti-inflammatory, hemostatic and damage-repairing roles on the gingival and oral surfaces.

[0032] In some embodiments, the humectant includes at least one of sorbitol, glycerol, PEG-400 and propylene glycol; the abrasive includes at least one of hydrated silica, calcium carbonate or dicalcium phosphate; the foaming agent includes at least one of sodium lauryl sulfate, sodium lauroyl sarcosinate, cocamidopropyl betaine, sodium lauroyl glutamate and sodium methyl cocoyl taurate; the thickener includes at least one of cellulose gum, xanthan gum, carrageenan, hydroxyethyl cellulose and hydroxypropyl guar gum.

[0033] In some embodiments, the toothpaste further includes at least one of sweetening agent, stabilizer, preservative and colorant.

[0034] The sweetener includes at least one of sodium saccharin, sucralose, acesulfame potassium, and stevioside; the stabilizer includes at least one of sodium pyrophosphate, trisodium phosphate, sodium dihydrogen pyrophosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium silicate, sodium carbonate, and sodium bicarbonate; the preservative includes at least one of sodium benzoate, methylparaben, propylparaben, benzyl alcohol, phenoxyethanol, ethylhexylglycerin, and p-hydroxyacetophenone; the colorant includes at least one of titanium dioxide and pigments.

[0035] In some embodiments, the toothpaste further includes essence.

[0036] In some embodiments, the toothpaste further includes an active ingredient, and the active ingredient includes at least one of fluoride, anti-sensitivity agent, hydroxyapatite, xylitol, and arginine.

[0037] The regenerated protein composition can be used in a silica or calcium carbonate or calcium hydrogen phosphate abrasive system, and can be added to efficacy formulations such as anti-caries or anti-sensitivity or anti-dental plaque, etc., additionally increasing the efficacy of the toothpaste in reducing inflammation and stopping bleeding and repairing damage.

[0038] The second aspect of the present invention also provides a preparation method of the above toothpaste, including the following steps:

[0039] Step 1: Dissolve the regenerated protein, film-forming agent, polyphosphate, and water-soluble raw materials in water to obtain a first premix.

[0040] Step 2: Premix the abrasive, adsorbent, and powder raw materials (such as thickener, powdered foaming agent) to obtain a second premix.

[0041] Step 3: Vacuum mix the humectant with the first premix, and then sequentially add the second premix, liquid foaming agent, and essence, and homogenize and degas to obtain the finished product.

[0042] In the above method, if there is a liquid foaming agent, it is added in Step 3; if not, the feeding process of the corresponding liquid foaming agent is omitted.

[0043] In some embodiments, in Step 3, the vacuum degree is -0.08 to -0.1 MPa, the homogenization rotation speed is 900 - 1100 rpm, and the temperature is 25 - 40 °C.

[0044] The present invention has the following beneficial effects compared with the prior art:

[0045] The present invention adds a regenerated protein composition to the toothpaste. The adsorbent is used to adsorb plaque debris, combined with the chelating and dispersing effect of polyphosphate, which improves the cleaning power. The film-forming agent prolongs the residence of the regenerated protein on the surface of the gums and oral mucosa, and synergistically with the regenerated protein helps to stop bleeding, reduce inflammation, and regenerate damaged tissues, realizing the synergistic effect of cleaning and repair, and having good application prospects. Detailed implementation mode

[0046] The following will combine the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work belong to the scope of protection of the present invention.

[0047] Unless otherwise defined, all technical terms and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the embodiments of the present invention belong. If the definitions stated in this part are contrary to or inconsistent with other aspects of the definitions stated in the patents, patent applications, published patent applications, and other publications incorporated herein by reference, the definitions listed in this part shall prevail over the definitions incorporated herein by reference.

[0048] Unless otherwise specified, the methods used in the following examples are all conventional methods. The materials, reagents, and instruments used, unless otherwise specified, are all conventional materials, reagents, and instruments in this field, and those skilled in the art can obtain them through commercial channels.

[0049] When an equivalent, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper preferred values and lower preferred values, it should be understood that all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value are specifically disclosed, regardless of whether the range is separately disclosed. For example, when the range "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described herein, unless otherwise stated, the range is intended to include its end values and all integers and fractions within the range. In the specification and claims of this application, range limitations can be combined and / or interchanged, and if not otherwise stated, these ranges include all sub-ranges contained therein.

[0050] Example 1

[0051] This example provides a toothpaste with a silica abrasive system

[0052] The formula is as follows:

[0053] Regenerated protein composition:

[0054] Silk fibroin (regenerated protein): 0.4 parts

[0055] Carboxymethyl chitosan (film-forming agent): 0.6 parts

[0056] 4A sodium molecular sieve (adsorbent): 0.8 parts

[0057] Sodium pyrophosphate (polyphosphate): 0.6 parts

[0058] Basic formula:

[0059] Humectant (20 parts of glycerol, 70 parts of sorbitol, 4 parts of PEG-400)

[0060] Abrasive (42 parts of hydrated silica)

[0061] Foaming agent (2.6 parts of sodium lauryl sulfate, 0.6 parts of sodium lauroyl sarcosinate)

[0062] Thickener (2.2 parts of cellulose gum)

[0063] Water 46 parts

[0064] Preservative 1 part, essence 2.4 parts, sucralose 0.32 parts, titanium dioxide 0.6 parts, cocamidopropyl betaine 4 parts

[0065] Preparation method:

[0066] First premix: Silk fibroin, carboxymethyl chitosan, sodium pyrophosphate, sucralose are dissolved in water and stirred until completely dispersed and dissolved.

[0067] Second premix: The abrasive, molecular sieve, thickener, and foaming agent are dry-mixed evenly.

[0068] Vacuum paste making:

[0069] Add the humectant and the first premix to the vacuum paste making pot (vacuum degree -0.09 MPa) and stir to mix evenly.

[0070] Inhale the second premix and stir and homogenize for 20 minutes (rotation speed 1000 rpm, temperature 30 °C).

[0071] Add cocamidopropyl betaine, essence, and titanium dioxide dispersion, stir and homogenize to degas and then break the vacuum.

[0072] Example 2

[0073] This example provides a toothpaste with a calcium carbonate abrasive system

[0074] The formula is as follows:

[0075] Regenerated protein composition:

[0076] Silk fibroin: 0.1 part

[0077] Carboxymethyl chitosan: 0.1 part

[0078] 4A sodium molecular sieve: 0.1 part

[0079] Sodium pyrophosphate: 1 part

[0080] Basic formulation:

[0081] Humectant (46 parts of sorbitol, 6 parts of PEG - 400)

[0082] Abrasive (84 parts of calcium carbonate, 9 parts of hydrated silica)

[0083] Foaming agent (4.6 parts of sodium lauryl sulfate)

[0084] Thickening agent (2.4 parts of cellulose gum)

[0085] 44 parts of water

[0086] 0.3 part of preservative, 2.2 parts of essence, 0.5 part of sodium saccharin

[0087] Preparation method:

[0088] Same as Example 1, adjust the abrasive to calcium carbonate + hydrated silica, and omit the colorant.

[0089] Example 3

[0090] This example provides a toothpaste with a calcium hydrogen phosphate abrasive system.

[0091] The formulation is as follows:

[0092] Regenerated protein composition:

[0093] 1 part of fibroin

[0094] 2 parts of carboxymethyl chitosan

[0095] 3 parts of molecular sieve

[0096] 1 part of sodium pyrophosphate

[0097] Basic formulation:

[0098] Humectant (16 parts of glycerol, 32 parts of sorbitol, 4 parts of PEG - 400)

[0099] Abrasive (84 parts of calcium hydrogen phosphate, 5 parts of hydrated silica)

[0100] Foaming agent (2.6 parts of sodium lauryl sulfate, 0.6 part of sodium lauroyl sarcosinate)

[0101] Thickening agent (2 parts of hydroxyethyl cellulose)

[0102] 42 parts of water

[0103] 1 part of preservative, 2.2 parts of essence, 0.36 part of sucralose, 4 parts of cocamidopropyl betaine. Preparation method:

[0104] Same as Example 1.

[0105] Comparative Example 1

[0106] The toothpaste of this comparative example does not contain adsorbent and polyphosphate

[0107] Components:

[0108] The adsorbent and polyphosphate were removed, and the rest was the same as in Example 1.

[0109] Comparative Example 2

[0110] The toothpaste of this comparative example does not contain regenerated protein and film-forming agent

[0111] Components:

[0112] The regenerated protein and the film-forming agent were removed, and the rest was the same as in Example 1.

[0113] Comparative Example 3

[0114] This comparative example uses conventional fluoride toothpaste

[0115] Components:

[0116] 0.5 parts of sodium fluoride was added, and the regenerated protein composition was removed. The rest was the same as in Example 1.

[0117] The toothpaste samples prepared in the above examples and comparative examples were respectively tested for their effects.

[0118] In order to verify the effectiveness of toothpaste containing a regenerated protein composition in improving gum problems, a subjective user trial survey was conducted. Users who reported having gum problems (red and swollen gums, bleeding gums, pain, etc.) were collected and randomly divided into three groups (Example 1 group - containing a regenerated protein composition, Comparative Example 1 - without adsorbents and pyrophosphates, Comparative Example 2 - without regenerated protein and film-forming agents). They brushed their teeth every morning and evening for two consecutive weeks. Fill out the questionnaire after completion. If the inflammation and bleeding subsided within 3 to 5 days of brushing, fill in "significant effect"; if the gum problem improved within 1 to 2 weeks of brushing, fill in "effective". If there is still no improvement after 2 weeks, fill in "ineffective". The statistical results are as follows:

[0119]

[0120] Note: Effective rate % = (number of people with significant effect + number of people with improvement) / number of people with valid questionnaires collected

[0121] From the results of the trial questionnaire, it can be seen that the toothpaste containing regenerated protein and a film-forming agent (Example 1, Comparative Example 1) has a significantly better effect on improving gum problems than the toothpaste without regenerated protein and a film-forming agent (Comparative Example 2). The effect of the toothpaste containing the regenerated protein composition on improving gum problems (especially in terms of marked effect) is significantly better than the toothpaste containing only regenerated protein and a film-forming agent but not an adsorbent and polyphosphate (Comparative Example 1).

[0122] To verify the repair effect of silk fibroin on damage, a cell scratch test was conducted. Human gingival fibroblasts with a viability of 85% or more were selected for the scratch experiment, and the migration rates of human gingival fibroblasts at 24 hours and 48 hours were observed under different concentrations of silk fibroin. Compared with the blank sample, the change in the scratch area was calculated. The results showed that at concentrations of 1%, 0.5%, 0.2%, 0.1%, and 0.05%, the cell viability was above 85% and all had a repair effect.

[0123] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A toothpaste containing a regenerated protein composition, characterized in that: The composition comprises the following components in parts by weight: Regeneration protein composition: 0.2-10 parts Moisturizer: 15-70 parts Abrasive: 10-50 parts Foaming agent: 0.5-10 parts Thickener: 0.5-3 parts Water: 8-40 parts; The regenerated protein composition consists of regenerated protein, film-forming agent, adsorbent and polyphosphate.

2. The toothpaste containing the regenerated protein composition as claimed in claim 1, characterized in that: The weight proportions of the components in the regenerated protein composition are: Regenerated protein: 0.05-1 part Film former: 0.05-3 parts Adsorbent: 0.05-3 parts Polyphosphate: 0.05-3 parts.

3. The toothpaste containing the regenerated protein composition as claimed in claim 1, characterized in that: The regenerated protein is at least one of silk fibroin, hydrolyzed collagen and hyaluronic acid.

4. The toothpaste containing the regenerated protein composition as claimed in claim 1, characterized in that: The film-forming agent is one of carboxymethyl chitosan, polyvinyl pyrrolidone, vinyl methyl ether / maleic anhydride copolymer and poloxamer 407.

5. The toothpaste containing the regenerated protein composition as claimed in claim 1, characterized in that: The adsorbent is one of molecular sieve, diatomaceous earth and beta-cyclodextrin.

6. The toothpaste containing the regenerated protein composition as claimed in claim 1, characterized in that: The polyphosphate is at least one of sodium salts or potassium salts such as tetrasodium pyrophosphate, sodium hexametaphosphate, sodium phytate, trisodium phosphate, and disodium dihydrogen pyrophosphate.

7. The toothpaste containing the regenerated protein composition as claimed in claim 1, characterized in that: The preferred weight percentages of the components in the regenerated protein composition are: Silk fibroin: 0.05-1 part Carboxymethyl chitosan: 0.05-2 parts 4A sodium molecular sieve: 0.05-3 parts Polyphosphate: 0.05-1 part.

8. The toothpaste containing the regenerated protein composition as claimed in claim 1, characterized in that: The moisturizer includes at least one of sorbitol, glycerin, PEG-400 and propylene glycol; the abrasive includes at least one of hydrated silica, calcium carbonate or calcium hydrogen phosphate; the foaming agent includes at least one of sodium lauryl sulfate, sodium lauroyl sarcosinate, cocamidopropyl betaine, sodium lauroyl glutamate and sodium methyl cocoyl taurate; the thickener includes at least one of cellulose gum, xanthan gum, carrageenan, hydroxyethyl cellulose and hydroxypropyl guar gum.

9. The method for preparing a toothpaste containing a regenerated protein composition as claimed in any one of claims 1 to 8, characterized in that: The steps include: Step 1, dissolving the regenerated protein, the film-forming agent, the polyphosphate and the water-soluble raw material in water to prepare a first premix; Step 2: premixing the abrasive, the adsorbent and the powder raw material to prepare a second premix; Step 3: After vacuum mixing the moisturizer and the first premix, the second premix, the liquid foaming agent and the flavor are added in sequence, and the finished product is obtained by homogenizing and degassing.

10. The method for preparing a toothpaste containing a regenerated protein composition as claimed in claim 9, characterized in that: In step 3, the vacuum degree is -0.08 to -0.1 MPa, the homogenization speed is 900-1100 rpm, and the temperature is 25-40°C.

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