Anti-tumor N-acetyl-D-glucosamine compound medicinal liquor and preparation method thereof

Abstract

The invention discloses an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor and a preparation method thereof, and relates to the technical field of biological medicines. The anti-tumor N-acetyl-D-glucosamine compound medicinal liquor is prepared from the following components: N-acetyl-D-glucosamine, taurine, flavone metal chelate, pterostilbene lipidosome, astragalus membranaceus slices, hawthorn slices, ganoderma lucidum slices, ginseng slices, quercetin, cordyceps sinensis and pure grain liquor. The N-acetyl-D-glucosamine and the taurine which are added in proportion have a synergistic effect and can enhance the anti-tumor effect of the medicinal liquor, the flavone metal chelate and the flavone compound are added for chelating with metal ions, tumor cell apoptosis can be induced through the multi-target effect, the tumor inhibition capacity is enhanced, and after the medicinal liquor is prepared, the anti-tumor effect of the medicinal liquor is improved. The flavone metal chelate is gradually released along with slow metabolism of alcohol, so that a slow release effect is realized, and the action time of effective components in a body is prolonged.

Description

Technical Field

[0001] The present invention relates to the field of biological medicine technology, and specifically to an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor and a preparation method thereof. Background Art

[0002] Tumor has become one of the major diseases seriously threatening life and health, and its incidence rate is increasing year by year. At present, the pathogenesis of tumors is not fully understood; N-acetyl-D-glucosamine is a new type of biochemical drug and also the basic constituent unit of various polysaccharides in organisms. It has the highest content in the exoskeletons of crustaceans. Clinically, it can be used to treat diseases such as rheumatic and rheumatoid arthritis, and also has antioxidant properties. It can be used as a food antioxidant, additive, and sweetener for diabetic patients, etc. It can enhance the function of the human immune system, inhibit the growth of cancer cells, and has inhibitory and therapeutic effects on cancer, tumors, or various inflammations; Taurine is an organic compound converted from sulfur-containing amino acids, also known as taurine, taurocholic acid, and choline. It is widely distributed in various tissues and organs of the body and exists in the interstitial fluid and intracellular fluid in a free state. Taurine is an active substance that regulates the normal physiological activities of the body and has functions such as anti-inflammatory, analgesic, blood sugar lowering, maintaining the osmotic pressure balance of the body, regulating lipid digestion and absorption, and regulating immunity.

[0003] CN201310159946.8 discloses an anti-tumor drug composition and a preparation method thereof, which mix traditional Chinese medicine components with pure grain liquor, seal and soak them to obtain an anti-tumor composition, but the synergistic effect between the components is weak; Patent CN201810060492.1 discloses a drug composition with anti-tumor effects and its medicinal liquor, which uses N-acetyl-D-glucosamine and taurine as raw materials, controls the ratio of the two, and makes a medicinal liquor with anti-tumor effects, but the active ingredients are few.

[0004] Therefore, we propose an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor and a preparation method thereof. Summary of the Invention

[0005] The purpose of the present invention is to provide an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor and a preparation method thereof to solve the problems raised in the prior art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: An anti-tumor N-acetyl-D-glucosamine compound medicinal liquor, comprising the following mass components: 18 - 20 parts of N-acetyl-D-glucosamine, 2 - 3 parts of taurine, 1 - 5 parts of flavonoid metal chelate, 1 - 3 parts of pterostilbene liposome, 2 - 4 parts of astragalus slices, 2 - 4 parts of hawthorn slices, 2 - 4 parts of ganoderma slices, 1 - 3 parts of ginseng slices, 1 - 3 parts of quercetin, 1 - 3 parts of cordyceps sinensis, and 48 - 69 parts of pure grain liquor;

[0007] The flavonoid metal chelate is prepared by chelating a flavonoid compound with a metal ion;

[0008] The pterostilbene liposome is prepared by encapsulating pterostilbene with liposomes.

[0009] In the above technical solution, N-acetyl-D-glucosamine inhibits tumor cells by enhancing the immune system function, hindering the adhesion and invasion of tumor cells to the stroma; taurine and N-acetyl-D-glucosamine added in a certain proportion have a synergistic effect, with effects such as anti-free radical and antioxidant, which helps to enhance human immunity and has a certain inhibitory effect on tumors; the astragalus polysaccharide in astragalus membranaceus tablets can activate macrophages and enhance the immune ability against tumors, and components such as astragaloside can block the cancer cell division cycle and delay tumor progression; the plant polyphenols in hawthorn tablets reduce cell damage by scavenging free radicals, slow down the growth of cancer cells, enhance the immune ability, and indirectly assist in inhibiting tumor development; the ganoderma polysaccharide and triterpenoid compounds in ganoderma lucidum tablets can interfere with the DNA synthesis of tumor cells, inhibit proliferation and induce apoptosis, and can also activate T lymphocytes, enhance the anti-tumor response, improve the fatigue state of patients, and enhance immunity; ginseng tablets can increase the white blood cell level of patients, relieve symptoms such as anemia and fatigue, and in combination with anti-tumor drugs, can enhance the killing effect on tumor cells and reduce drug resistance; quercetin can scavenge free radicals in the body, prevent cardiovascular diseases, nervous system diseases, etc., and improve the immunity of patients by inhibiting neutrophil aggregation and the release of inflammatory factors; the cordyceps polysaccharide and adenosine components of cordyceps sinensis can promote lymphocyte proliferation, improve the immune function of tumor patients, assist in blocking the RNA synthesis of tumor cells, and reduce the expansion rate of cancer cells such as lung cancer.

[0010] A preparation method of an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor includes the following steps:

[0011] Take pure grain liquor, first add 1 / 2 mass of taurine, stir to dissolve, cool down to 5 - 10 °C, then add N-acetyl-D-glucosamine, flavonoid metal chelate, pterostilbene liposome, astragalus membranaceus tablets, hawthorn tablets, ganoderma lucidum tablets, ginseng tablets, quercetin, cordyceps sinensis, stir evenly, carry out a low-temperature reaction, then add the remaining taurine, filter, fill, and let stand at low temperature to obtain the medicinal liquor.

[0012] Further, the conditions for the low-temperature reaction are: temperature 5 - 10 °C, time 1 - 3 h.

[0013] Further, the conditions for the low-temperature standing are: temperature 5 - 10 °C, time 10 - 20 days.

[0014] Further, the flavonoid metal chelate is prepared by the following process:

[0015] The flavonoid compound and the metal inorganic salt are mixed to obtain a mixture, which is dissolved in an ethanol aqueous solution, heated for reaction, and subjected to reduced pressure distillation to obtain a flavonoid metal chelate.

[0016] Furthermore, the mass ratio of the flavonoid compound to the metal inorganic salt is 2:(1-2);

[0017] The mass ratio of the mixture to the ethanol aqueous solution is 1:(5-15).

[0018] Furthermore, the process conditions of the heating reaction are: temperature 40-60° C., time 8-12 h.

[0019] Furthermore, the mass fraction of the ethanol aqueous solution is 40-60%.

[0020] Furthermore, the flavonoid compound is a mixture of one or more of luteolin, apigenin, hesperidin and diosgenin.

[0021] Furthermore, the metal inorganic salt is a mixture of one or more of copper sulfate, copper chloride, ferrous chloride, ferrous sulfate, zinc sulfate, and zinc chloride.

[0022] In the above technical scheme, flavonoids can inhibit the growth, development and spread of cancer cells, and induce apoptosis of various cancer cells, and have good anti-tumor activity. Metal ions themselves have certain anti-cancer activity. After the two are chelated, they can induce tumor cell apoptosis through multi-target effects and enhance the ability to inhibit tumors. After being made into an alcoholic solution, the flavonoid metal chelate is gradually released with the slow metabolism of alcohol, achieving a sustained-release effect and prolonging the action time of the effective ingredients in the body. In addition, N-acetyl-D-glucosamine is a polysaccharide, which can synergize with flavonoid metal chelates to jointly enhance the anti-tumor effect.

[0023] Furthermore, the pterostilbene liposome is prepared by the following process:

[0024] Lecithin, cholesterol, pterostilbene and chloroform are mixed, stirred until dissolved, then evaporated to dryness to form a film, PBS buffer solution is added, and ultrasonication is performed for 4 to 6 minutes to obtain pterostilbene liposomes.

[0025] Furthermore, the ratio of lecithin, cholesterol, pterostilbene, chloroform, and PBS buffer solution is (1-3) mg: (0.3-1) mg: (0.1-0.3) mg: (0.15-0.25) mL: (0.075-0.1) mL.

[0026] Furthermore, the temperature of evaporation and film formation is 45-65°C.

[0027] Furthermore, the pH of the PBS buffer solution is 6.5.

[0028] Further, the lecithin is one or a mixture of two of soy lecithin and egg yolk lecithin.

[0029] In the above technical solution, pterostilbene has antioxidant effects, reduces free radical damage, and also has anti-tumor and anti-inflammatory effects. However, its water solubility is poor. Therefore, it is encapsulated by a phospholipid bilayer to form a nanoscale carrier, enabling it to be evenly dispersed in the medicinal liquor, improving the bioavailability and stability of pterostilbene. The liposome can enhance intestinal absorption by mimicking the cell membrane structure, further enhancing the anti-tumor effect.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0031] 1. Flavonoid compounds can inhibit the growth, development, and spread of cancer cells, and at the same time induce apoptosis of various cancer cells, having good anti-tumor activity. Metal ions themselves have certain anti-cancer activity. After chelating the two, tumor cell apoptosis can be induced through multi-target actions, enhancing the inhibitory ability against tumors. After being made into a medicinal liquor, the flavonoid metal chelate is gradually released with the slow metabolism of alcohol, achieving a sustained release effect and prolonging the action time of the active ingredient in the body. In addition, N-acetyl-D-glucosamine belongs to polysaccharides and can synergistically act with the flavonoid metal chelate to jointly enhance the anti-tumor effect.

[0032] 2. Pterostilbene has antioxidant effects, reduces free radical damage, and also has anti-tumor and anti-inflammatory effects. However, its water solubility is poor. Therefore, it is encapsulated by a phospholipid bilayer to form a nanoscale carrier, enabling it to be evenly dispersed in the medicinal liquor, improving the bioavailability and stability of pterostilbene. The liposome can enhance intestinal absorption by mimicking the cell membrane structure, further enhancing the anti-tumor effect. Detailed implementation manners

[0033] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0034] In the following detailed implementation manners,

[0035] Astragalus membranaceus tablets, product number 0011, are sourced from Bozhou Tengwang Pharmaceutical Co., Ltd.;

[0036] Hawthorn tablets are sourced from An'guo Desheng Pharmaceutical Co., Ltd.;

[0037] Ganoderma lucidum tablets are sourced from Guanxian Guanyu Agriculture Co., Ltd.;

[0038] Ginseng slices, sourced from Bozhou Yongzheng Pharmaceutical Co., Ltd.;

[0039] Quercetin, product number HPS95 - 02, sourced from Xi'an Xufeng Biotechnology Co., Ltd.;

[0040] Cordyceps sinensis, sourced from Jilin Lusongtang Ginseng & Antler Co., Ltd.;

[0041] N - Acetyl - D - glucosamine, CAS number 29031 - 19 - 4, sourced from Nantong Yingruida Biotechnology Co., Ltd.;

[0042] Taurine, CAS number 107 - 35 - 7, sourced from Chongqing Tianrun Bioproducts Co., Ltd.;

[0043] Tangeretin, CAS number 481 - 53 - 8, sourced from Shanghai Yuanye Biotechnology Co., Ltd.;

[0044] Zinc sulfate, CAS number 7733 - 02 - 0, sourced from Xi'an Jinxiang Pharmaceutical Excipients Co., Ltd.;

[0045] Ethanol, CAS number 64 - 17 - 5, sourced from Jinan Ruishan Chemical Industry Co., Ltd.;

[0046] Soybean lecithin, CAS number 8002 - 43 - 5, 80 mesh, sourced from Shaanxi Bohong Health Industry Co., Ltd.;

[0047] Cholesterol, CAS number 57 - 88 - 5, sourced from Xi'an Jinxiang Pharmaceutical Excipients Co., Ltd.;

[0048] Pterostilbene, CAS number 537 - 42 - 8, 80 mesh, sourced from Shaanxi Bohong Health Industry Co., Ltd.;

[0049] Chloroform, CAS number 67 - 66 - 3, sourced from Merck reagent;

[0050] PBS buffer solution, pH 6.5, product number T17705.500, sourced from Shanghai Shangbao Biotechnology Co., Ltd.;

[0051] Pure grain liquor, 52°, sourced from Sichuan Shangdengyuan Wine Industry Co., Ltd.

[0052] Example 1: A preparation method of an anti - tumor N - acetyl - D - glucosamine compound liquor, comprising the following steps:

[0053] (1) Preparation of flavonoid metal chelate:

[0054] Mix hesperetin and zinc sulfate to obtain a mixture, dissolve it in an ethanol aqueous solution, heat for reaction, and perform vacuum distillation to obtain a flavonoid metal chelate; the mass ratio of hesperetin to zinc sulfate is 2:2; the mass ratio of the mixture to the ethanol aqueous solution is 1:15; the process conditions for the heating reaction are: temperature 60°C, time 12 h; the mass fraction of the ethanol aqueous solution is 60%;

[0055] (2) Preparation of pterostilbene liposomes:

[0056] Mix lecithin, cholesterol, pterostilbene and chloroform, stir until dissolved, then evaporate to dryness to form a film, add PBS buffer solution, and ultrasonicate for 6 min to obtain pterostilbene liposomes; the ratio of lecithin, cholesterol, pterostilbene, chloroform, and PBS buffer solution is 3 mg: 1 mg: 0.3 mg: 0.25 mL: 0.1 mL; the temperature for evaporating to dryness to form a film is 65°C;

[0057] (3) Preparation of medicinal liquor:

[0058] Take pure grain liquor, first add 1 / 2 of the mass of taurine, stir to dissolve, cool down to 10°C, then add N-acetyl-D-glucosamine, flavonoid metal chelate, pterostilbene liposomes, astragalus slices, hawthorn slices, ganoderma slices, ginseng slices, quercetin, cordyceps sinensis, stir evenly, let stand at 10°C for 3 h, then add the remaining taurine, filter, fill, and store at 10°C for 20 days to obtain the medicinal liquor; the mass ratio of N-acetyl-D-glucosamine, taurine, flavonoid metal chelate, pterostilbene liposomes, astragalus slices, hawthorn slices, ganoderma slices, ginseng slices, quercetin, cordyceps sinensis, and pure grain liquor is 20:3:5:3:4:4:4:3:3:3:48.

[0059] Example 2: A preparation method of an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor, comprising the following steps:

[0060] (1) Preparation of flavonoid metal chelate:

[0061] Mix hesperetin and zinc sulfate to obtain a mixture, dissolve it in an ethanol aqueous solution, heat for reaction, and perform vacuum distillation to obtain a flavonoid metal chelate; the mass ratio of hesperetin to zinc sulfate is 2:1.5; the mass ratio of the mixture to the ethanol aqueous solution is 1:10; the process conditions for the heating reaction are: temperature 50°C, time 10 h; the mass fraction of the ethanol aqueous solution is 50%;

[0062] (2) Preparation of pterostilbene liposomes:

[0063] Mix lecithin, cholesterol, pterostilbene, and chloroform, stir until dissolved, then evaporate to dryness to form a film. Add PBS buffer solution and sonicate for 5 min to obtain pterostilbene liposomes. The ratio of lecithin, cholesterol, pterostilbene, chloroform, and PBS buffer solution is 2 mg: 0.7 mg: 0.2 mg: 0.2 mL: 0.085 mL. The temperature for evaporating to dryness to form a film is 55 °C.

[0064] (3) Preparation of the medicinal liquor:

[0065] Take pure grain liquor, first add 1 / 2 of the mass of taurine, stir to dissolve, cool down to 8 °C, then add N-acetyl-D-glucosamine, flavonoid metal chelate, pterostilbene liposomes, astragalus slices, hawthorn slices, ganoderma lucidum slices, ginseng slices, quercetin, cordyceps sinensis, stir evenly, let stand at 8 °C for 2 h, then add the remaining taurine, filter, fill, and store at 8 °C for 15 days to obtain the medicinal liquor. The mass ratio of N-acetyl-D-glucosamine, taurine, flavonoid metal chelate, pterostilbene liposomes, astragalus slices, hawthorn slices, ganoderma lucidum slices, ginseng slices, quercetin, cordyceps sinensis, and pure grain liquor is 19: 2: 3: 2: 3: 3: 3: 2: 2: 2: 59.

[0066] Example 3: A preparation method of an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor, comprising the following steps:

[0067] (1) Preparation of the flavonoid metal chelate:

[0068] Mix hesperetin and zinc sulfate to obtain a mixture, dissolve it in an ethanol aqueous solution, heat and react, and distill under reduced pressure to obtain the flavonoid metal chelate. The mass ratio of hesperetin to zinc sulfate is 2: 1. The mass ratio of the mixture to the ethanol aqueous solution is 1: 5. The process conditions for heating and reacting are: temperature 40 °C, time 8 h. The mass fraction of the ethanol aqueous solution is 40%.

[0069] (2) Preparation of the pterostilbene liposomes:

[0070] Mix lecithin, cholesterol, pterostilbene, and chloroform, stir until dissolved, then evaporate to dryness to form a film. Add PBS buffer solution and sonicate for 4 min to obtain pterostilbene liposomes. The ratio of lecithin, cholesterol, pterostilbene, chloroform, and PBS buffer solution is 1 mg: 0.3 mg: 0.1 mg: 0.15 mL: 0.075 mL. The temperature for evaporating to dryness to form a film is 45 °C.

[0071] (3) Preparation of the medicinal liquor:

[0072] Take pure grain liquor, first add 1 / 2 of its mass of taurine, stir to dissolve, cool down to 5°C, then add N-acetyl-D-glucosamine, flavonoid metal chelate, pterostilbene liposome, astragalus slices, hawthorn slices, ganoderma lucidum slices, ginseng slices, quercetin, cordyceps sinensis, stir evenly, let stand at 5°C for 1 h, then add the remaining taurine, filter, fill into bottles, and store at 5°C for 10 days to obtain the medicinal liquor; the mass ratio of N-acetyl-D-glucosamine, taurine, flavonoid metal chelate, pterostilbene liposome, astragalus slices, hawthorn slices, ganoderma lucidum slices, ginseng slices, quercetin, cordyceps sinensis, and pure grain liquor is 18:2:1:1:2:2:2:1:1:1:69.

[0073] Comparative Example 1: Taking Example 1 as a comparison, without adding flavonoid metal chelate to the medicinal liquor and keeping other conditions unchanged, a preparation method of an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor is as follows:

[0074] (1) Preparation of pterostilbene liposome:

[0075] Mix lecithin, cholesterol, pterostilbene and chloroform, stir until dissolved, then evaporate to dryness to form a film, add PBS buffer solution, and sonicate for 6 min to obtain pterostilbene liposome; the ratio of lecithin, cholesterol, pterostilbene, chloroform, and PBS buffer solution is 3 mg: 1 mg: 0.3 mg: 0.25 mL: 0.1 mL; the temperature for evaporating to dryness to form a film is 65°C;

[0076] (2) Preparation of the medicinal liquor:

[0077] Take pure grain liquor, first add 1 / 2 of its mass of taurine, stir to dissolve, cool down to 10°C, then add N-acetyl-D-glucosamine, pterostilbene liposome, astragalus slices, hawthorn slices, ganoderma lucidum slices, ginseng slices, quercetin, cordyceps sinensis, stir evenly, let stand at 10°C for 3 h, then add the remaining taurine, filter, fill into bottles, and store at 10°C for 20 days to obtain the medicinal liquor; the mass ratio of N-acetyl-D-glucosamine, taurine, pterostilbene liposome, astragalus slices, hawthorn slices, ganoderma lucidum slices, ginseng slices, quercetin, cordyceps sinensis, and pure grain liquor is 20:3:3:4:4:4:3:3:3:53.

[0078] Comparative Example 2: Taking Example 1 as a comparison, without adding pterostilbene liposome to the medicinal liquor and keeping other conditions unchanged, a preparation method of an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor is as follows:

[0079] (1) Preparation of flavonoid metal chelate:

[0080] Mix hesperetin and zinc sulfate to obtain a mixture, dissolve it in an ethanol aqueous solution, heat for reaction, and perform vacuum distillation to obtain a flavonoid metal chelate; the mass ratio of hesperetin to zinc sulfate is 2:2; the mass ratio of the mixture to the ethanol aqueous solution is 1:15; the process conditions for the heating reaction are: temperature 60 °C, time 12 h; the mass fraction of the ethanol aqueous solution is 60%;

[0081] (2) Preparation of the medicinal liquor:

[0082] Take pure grain liquor, first add 1 / 2 of the mass of taurine, stir to dissolve, cool down to 10 °C, then add N-acetyl-D-glucosamine, flavonoid metal chelate, astragalus slices, hawthorn slices, ganoderma slices, ginseng slices, quercetin, cordyceps sinensis, stir evenly, let stand at 10 °C for 3 h, then add the remaining taurine, filter, fill, and store at 10 °C for 20 days to obtain the medicinal liquor; the mass ratio of N-acetyl-D-glucosamine, taurine, flavonoid metal chelate, astragalus slices, hawthorn slices, ganoderma slices, ginseng slices, quercetin, cordyceps sinensis, and pure grain liquor is 20:3:5:4:4:4:3:3:3:51.

[0083] Comparative Example 3: Taking Example 1 as a comparison, without adding pterostilbene liposomes and without adding flavonoid metal chelates, a preparation method of an anti-tumor N-acetyl-D-glucosamine compound medicinal liquor, with other conditions unchanged, the specific steps are as follows:

[0084] Take pure grain liquor, first add 1 / 2 of the mass of taurine, stir to dissolve, cool down to 10 °C, then add N-acetyl-D-glucosamine, astragalus slices, hawthorn slices, ganoderma slices, ginseng slices, quercetin, cordyceps sinensis, stir evenly, let stand at 10 °C for 3 h, then add the remaining taurine, filter, fill, and store at 10 °C for 20 days to obtain the medicinal liquor; the mass ratio of N-acetyl-D-glucosamine, taurine, astragalus slices, hawthorn slices, ganoderma slices, ginseng slices, quercetin, cordyceps sinensis, and pure grain liquor is 20:3:4:4:4:3:3:3:56.

[0085] Experiment:

[0086] Anti-tumor test: Take male ICR mice, 22 g, divide them into a blank control group, an example group, and a comparative example group, with 12 mice in each group, and perform W 256 intrahepatic inoculation. 7 days after inoculation, anesthetize the mice, perform laparotomy, and measure the maximum diameter a and the minimum diameter b of the tumor surface on the liver;

[0087] Then suture the mice. After the mice wake up, the example group and the comparative example group are given the medicinal liquors in Examples 1-3 and Comparative Examples 1-3 by gavage, with a dose of 0.3 g / kg, the blank control group is given an equal amount of normal saline, for 15 consecutive days, once a day. 24 h after the last gavage, calculate the tumor volume and take the average value;

[0088] Tumor volume = (a × b 2 ) / 2

[0089] Antioxidant capacity test: Mice were selected and divided into a blank control group, an example group, and a comparative example group, with 12 mice in each group. The example group and the comparative example group were given intragastric administration every day at a dose of 0.3 g / kg, and the blank control group was given an equal amount of normal saline for 14 consecutive days; 60 μL of blood was taken from the tails of the mice, and the SOD activity was measured with reference to the method of pyrogallol autoxidation;

[0090] The following table shows the test results of the anti-tumor and antioxidant capacities of the medicinal liquor;

[0091] Tumor volume before administration / mm Tumor volume after administration / mm SOD (μL / L) Example 1 4.22±0.52 4.66±0.41 44.35±1.97 Example 2 4.13±0.60 4.78±0.56 43.84±2.18 Example 3 4.34±0.55 4.85±0.34 42.49±2.35 Comparative Example 1 4.55±0.68 5.63±0.47 41.01±2.79 Comparative Example 2 4.36±0.77 5.51±0.51 38.96±2.84 Comparative Example 3 4.49±0.60 5.91±0.35 35.08±2.51 Blank control group 4.65±0.39 6.98±0.78 28.36±2.39

[0092] Based on the data in the above table, the following conclusions can be clearly drawn:

[0093] Examples 1 to 3 were compared with the medicinal liquors in Comparative Examples 1 to 3. From the test results,

[0094] Compared with Example 1, in Comparative Example 1, flavonoid metal chelates were not added to the medicinal liquor. After administration, the tumor volume increased more and the tumor-inhibiting ability decreased because flavonoids can inhibit the growth, development, and spread of cancer cells and at the same time induce apoptosis of various cancer cells, having good anti-tumor activity;

[0095] Compared with Example 1, in Comparative Example 2, pterostilbene liposomes were not added to the medicinal liquor. After administration, the tumor volume increased more and the SOD activity decreased more significantly, indicating a decrease in antioxidant capacity and a decrease in tumor-inhibiting ability because pterostilbene has an antioxidant effect and reduces free radical damage;

[0096] Compared with Example 1, in Comparative Example 3, neither pterostilbene liposomes nor flavonoid metal chelates were added, and the tumor-inhibiting ability and antioxidant capacity decreased more significantly. It can be seen that the settings of the process and the materials used in this application can promote the improvement of the anti-tumor and antioxidant capacities of the prepared medicinal liquor.

[0097] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, in any regard, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, it is intended to embrace all changes falling within the meaning and scope of the equivalent elements of the claims in the present invention.

Claims

1. An anti-tumor N-acetyl-D-glucosamine compound wine preparation, characterized in that: The composition comprises the following components by mass: 18-20 parts of N-acetyl-D-glucosamine, 2-3 parts of taurine, 1-5 parts of flavonoid metal chelate, 1-3 parts of pterostilbene liposome, 2-4 parts of astragalus slices, 2-4 parts of hawthorn slices, 2-4 parts of ganoderma lucidum slices, 1-3 parts of ginseng slices, 1-3 parts of quercetin, 1-3 parts of cordyceps sinensis, and 48-69 parts of pure grain wine; The flavonoid metal chelate is prepared by chelating flavonoid compounds with metal ions; The pterostilbene liposome is prepared by liposome encapsulating pterostilbene.

2. A method for preparing an anti-tumor N-acetyl-D-glucosamine compound wine, characterized in that: The following steps are involved: Take pure grain wine, first add 1 / 2 mass of taurine, stir to dissolve, cool to 5-10°C, then add N-acetyl-D-glucosamine, flavonoid metal chelate, pterostilbene liposome, astragalus, hawthorn, ganoderma lucidum, ginseng, quercetin, cordyceps sinensis, stir evenly, react at low temperature, then add the remaining taurine, filter, fill, and let stand at low temperature to obtain the wine.

3. The method for preparing an anti-tumor N-acetyl-D-glucosamine compound wine according to claim 2, characterized in that: The flavonoid metal chelate is prepared by the following process: The flavonoid compound and the metal inorganic salt are mixed to obtain a mixture, which is dissolved in an ethanol aqueous solution, heated for reaction, and subjected to reduced pressure distillation to obtain a flavonoid metal chelate.

4. The method for preparing an anti-tumor N-acetyl-D-glucosamine compound wine according to claim 2, characterized in that: The pterostilbene liposome is prepared by the following process: Lecithin, cholesterol, pterostilbene and chloroform are mixed, stirred until dissolved, then evaporated to dryness to form a film, PBS buffer solution is added, and ultrasonication is performed for 4 to 6 minutes to obtain pterostilbene liposomes.

5. The method for preparing an anti-tumor N-acetyl-D-glucosamine compound wine according to claim 3, characterized in that: The mass ratio of flavonoid compound to metal inorganic salt is 2:(1-2); The mass ratio of the mixture to the ethanol aqueous solution is 1:(5-15).

6. The method for preparing an anti-tumor N-acetyl-D-glucosamine compound wine according to claim 3, characterized in that: The process conditions of the heating reaction are: temperature 40-60°C, time 8-12h.

7. The method for preparing an anti-tumor N-acetyl-D-glucosamine compound wine according to claim 4, characterized in that: The ratio of lecithin, cholesterol, pterostilbene, chloroform, and PBS buffer solution is (1-3) mg: (0.3-1) mg: (0.1-0.3) mg: (0.15-0.25) mL: (0.075-0.1) mL.

8. The method for preparing an anti-tumor N-acetyl-D-glucosamine compound wine according to claim 4, characterized in that: The temperature for evaporation and film formation is 45-65°C.

9. The method for preparing an anti-tumor N-acetyl-D-glucosamine compound wine according to claim 3, characterized in that: The flavonoid compound is a mixture of one or more of luteolin, apigenin, hesperidin and diosgenin.

10. The method for preparing an anti-tumor N-acetyl-D-glucosamine compound wine according to claim 3, characterized in that: The metal inorganic salt is a mixture of one or more of copper sulfate, copper chloride, ferrous chloride, ferrous sulfate, zinc sulfate and zinc chloride.