Preparation method of ferrous sulfide (FeS) protein for treating fungal infection

Abstract

The present invention relates to a process for the preparation of ferrous sulfide (FeS) proteins for the treatment of fungal infections. The method comprises the following steps: extracting effective medicine components such as sulfur (S), protein and iron disulfide (FeS2) from traditional Chinese medicines such as natural copper, scorpion and sulfur, and dissolving and reacting with trichloroisocyanuric acid and a catalyst sodium hydroxide to generate the pyrite compound. The compound is combined with a plurality of receptors of fungal cells in a non-selective manner to destroy the functions of fungal cell membranes and mitochondria, so that the growth and reproduction of the fungal cells are inhibited. Experiments show that the prepared thioferritin has remarkable antifungal activity and is suitable for treating various intractable fungal infections. The technology can effectively solve the problems of strong drug resistance and high recurrence rate of the existing antifungal drugs.

Description

Technical Field

[0001] The present invention relates to the field of biomedicine, and particularly to a technology for preparing a sulfur-iron (FeS) protein complex by combining traditional Chinese medicine extraction with modern chemical reactions, which is particularly suitable for the treatment of fungal infections. Background Art

[0002] Existing antifungal drugs include polyene antibiotics, imidazoles, pyrimidines, allylamines, and echinocandins. Although these drugs have been widely used in the treatment of fungal infections, there are still multiple problems. Polyene antibiotics bind to ergosterol in the fungal cell membrane to form pores, causing the leakage of intracellular substances and leading to the death of fungal cells. However, this type of drug also acts on cholesterol in mammalian cell membranes, has large toxic and side effects, and some fungi are resistant to this type of drug. Imidazoles and allylamine drugs can selectively inhibit the growth of fungal cells, but they have strong drug resistance and a high recurrence rate, and the clinical treatment effect is not good.

[0003] The present invention aims to overcome the defects of the existing technology and provide a new traditional Chinese medicine complex - sulfur-iron (FeS) protein, which has higher antifungal activity and can effectively treat refractory fungal infections, especially fungal diseases with strong drug resistance and high recurrence rate. Summary of the Invention

[0004] The purpose of the present invention is to provide a preparation method of sulfur-iron (FeS) protein for the treatment of fungal infections to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A preparation method of sulfur-iron (FeS) protein for the treatment of fungal infections, comprising the following steps:

[0006] (1) Extract proteins from the traditional Chinese medicines scorpion, centipede, and ledebouriella root.

[0007] (2) Dissolve sulfur, pyrite, trichloroisocyanuric acid, and FeS2 to generate a sulfur-iron protein complex.

[0008] (3) React under the catalysis of sodium hydroxide to finally generate sulfur-iron protein.

[0009] Preferably, the active ingredients sulfur (S), protein, and iron disulfide (FeS2) extracted from the traditional Chinese medicine react with trichloroisocyanuric acid to generate an acidic complex, and the sulfur-iron protein complex is obtained through chemical synthesis.

[0010] Preferably, the temperature of the catalytic reaction is controlled between 40°C and 50°C, and the generation of sulfur-iron protein is promoted within this temperature range.

[0011] Preferably, when the trichloroisocyanuric acid reacts with iron disulfide (FeS2), a sulfur-iron protein with weak acidity and capable of binding to various fungal cell receptors is generated.

[0012] Preferably, the generated sulfur-iron protein complex binds to Complex I of the oxidative respiratory chain and Complex III of cytochrome oxidase in the mitochondria of fungal cells, inhibiting oxidative phosphorylation in fungal cells and thus reducing ATP synthesis.

[0013] Preferably, the generated sulfur-iron protein complex kills fungi by disrupting the fungal cell membrane, causing it to dehydrate, denature, and rupture rapidly.

[0014] Preferably, the molecular formula of the sulfur-iron protein complex is CI3N3O3S3, with a characteristic molecular weight of 340.695, and it forms a brown precipitate by dissolving iron disulfide (FeS2).

[0015] A sulfur-iron protein complex according to claim 1, for treating fungal infections, characterized in that it binds to multiple receptors such as capsular proteins, cell walls, cell membranes, and protein synthesis disorders on the surface of fungi, disrupting the structure and function of fungal cells, and thus achieving the antifungal treatment effect.

[0016] Compared with the prior art, the beneficial effects of the present invention are:

[0017] 1. Broad-spectrum antifungal activity: By binding to multiple receptors on the surface of fungal cells, the sulfur-iron protein complex of the present invention can act on various different types of fungi, especially showing excellent effects in treating refractory fungal infections, with a wide range of adaptability.

[0018] 2. Reducing drug resistance: The complex acts on fungal cells simultaneously through multiple targets (mitochondria, cell membrane, cell wall, and protein synthesis system), effectively reducing the possibility of fungi developing drug resistance and overcoming the problem of high drug resistance of existing antifungal drugs.

[0019] 3. Efficient energy inhibition: The sulfur-iron protein complex can interfere with the oxidative respiratory chain of fungal mitochondria, inhibit ATP synthesis, and cause fungal cells to rapidly lose their energy metabolism ability, thus achieving the effect of rapidly killing fungi.

[0020] 4. Cell membrane disruption: By disrupting the fungal cell membrane, promoting the loss of intracellular substances, and causing the cells to lose water and denature, it directly leads to the apoptosis of fungal cells. This direct physical and chemical damage can effectively accelerate the treatment process and reduce the chance of disease recurrence.

[0021] 5. Combination of natural Chinese medicine and chemical synthesis: The present invention combines natural Chinese medicine ingredients (such as scorpion, centipede, pyrite, etc.) with modern chemical reactions (synthesis of sulfur-iron protein complex), which not only enhances the antifungal effect of the drug, but also reduces the toxic and side effects, and is safer than traditional drugs.

[0022] 6. Significant clinical effect: Experiments and clinical applications show that the sulfur-iron protein complex has a significant effect in the treatment of chronic and refractory fungal infections, can significantly shorten the treatment cycle, and reduce the recurrence rate.

[0023] 7. Simple operation and low cost: The preparation process of this method is simple, the required equipment and reaction conditions are easy to achieve, suitable for large-scale production, reducing the production cost, and facilitating popularization and application. Detailed implementation method

[0024] The technical solutions in the embodiments of the present invention are clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative work belong to the protection scope of the present invention.

[0025] The preparation method of the sulfur-iron protein of the present invention includes the following steps:

[0026] 1. Chinese medicine extraction: Proteins are extracted from Chinese medicine materials scorpion, centipede, and ledebouriella seseloides as active drug ingredients. These Chinese medicines are extracted for drug active ingredients through traditional Chinese medicine decoction methods, especially proteins containing high sulfur elements.

[0027] 2. Mineral medicine extraction: Extract the effective ingredients from mineral medicines. The sulfur (S) and iron disulfide (FeS2) components are respectively extracted from Chinese medicine mineral medicines such as sulfur, pyrite, and magnetite. After the iron disulfide in pyrite reacts with dilute hydrochloric acid, the generated iron disulfide that does not dissolve in dilute hydrochloric acid is separated by shaking, but decomposes into iron sulfide (FeS) after adding sodium hydroxide.

[0028] 3. Chemical reaction: Dissolve the extracted Chinese medicine components in trichloroisocyanuric acid and mix well by shaking. Subsequently, FeS2 is added to form a complex of trichlorocyanuric acid compound and FeS2, an acidic compound.

[0029] 4. Catalytic reaction: Add the acidified iron disulfide precipitate to a catalyst (sodium hydroxide), and add the protein extracted from Chinese medicine. During the reaction, a large amount of heat energy is generated, and finally decomposes into a brown precipitate - iron sulfide (FeS) protein, forming a composite compound.

[0030] Drug action mechanism

[0031] Iron sulfide (FeS) protein acts on fungal cells through multiple targets. The specific mechanisms include:

[0032] Acting on fungal mitochondria: Iron sulfide protein binds to complex I of the oxidative respiratory chain and complex III of cytochrome oxidase in fungal mitochondria, hinders the electron transfer of substrates, increases the O / P ratio of oxidative phosphorylation, inhibits the synthesis of the energy agent adenosine triphosphate (ATP), and leads to insufficient energy supply in fungal cells.

[0033] Destroying the fungal cell membrane: This complex binds to the lipid capsular protein complex, cell wall, and cell membrane on the surface of fungal cells, causing the fungal cells to rapidly dehydrate, denature, and burn into a charred brown color. The cell wall gap widens, the cell membrane ruptures, promoting the entry of traditional Chinese medicine toxic proteins and iron sulfide proteins into the cell interior, and ultimately leading to the death of fungal cells.

[0034] Interfering with fungal protein synthesis: The iron sulfide protein complex also inhibits the protein reverse transcription of fungal ribosomal RNA (rRNA), messenger RNA (mRNA), and transfer RNA (tRNA) by affecting the S30 segment of fungal protein subunits, thereby interfering with fungal protein synthesis and hindering its growth and reproduction.

[0035] Examples

[0036] Example 1

[0037] During the physical examination of patient Ning XX at Yongzhou Central Hospital in 2023, it was shown that: cardiac function was grade 2, with multiple lesions such as uterine adenomyoma, hypertrophy, cysts, effusion, chronic cervicitis, leucorrhea grade III, pus cells and mixed bacteria, fungal vaginitis, and breast nodules. On March 19, 2024, the patient came to our company for treatment. The previous treatment with general drugs had no obvious effect. The treatment with trichloroisocyanuric acid starting from April 28 was still not satisfactory. Passively, the treatment with the present invention was adopted on July 26, and the patient was cured on October 14.

[0038] Example 2

[0039] Patient Zhuang XX was infected with syphilis 20 years ago, had 2 abortions, had a positive pregnancy test (hcG) after 2 surgical uterine evacuations for ectopic pregnancy, and was passively injected with chemotherapy drugs (specific drugs unknown). In July 2023, due to a left humeral fracture, pelvic inflammation in gynecology, severe itching in the ears, nose, eyes, and vulva, and pain in the lower limb joints, etc., the patient came to our company to receive the pairing of biological medicine and traditional Chinese medicine. After treatment for up to one year, the above-mentioned symptoms were basically recovered, but problems such as local itching in the ears, nose, eyes, vulva, enuresis, and simple high anal fistula still remained unresolved. On April 28, 2024, the patient was passively treated with the drug of the present invention. After actively using the drug, there was an obvious therapeutic effect. During the reexamination on October 12, 2024, all clinical symptoms recovered. In this case, the patient actively received the treatment with this technology for more than one year and achieved complete recovery. After treatment, the symptoms gradually disappeared, and the clinical reexamination showed that all symptoms recovered.

[0040] Example 3

[0041] Patient Li XX came to our company for the treatment of eczema on the forearm and behind the ear through Douyin information in March 2023. After the eczema on the arm was cured, in January 2024, the patient was treated for rashes on both lower limbs and behind the ears. This case is a patient with a combined secondary infection of fungi, bacteria, and viruses. After being diagnosed by pathogenic microorganisms, the patient actively received the rehabilitation treatment of Xuguang Technology Biomedicine (vaccine + bacterin) on October 13, 2024.

[0042] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing an iron sulfide (FeS) protein for treating fungal infections, comprising the following steps: (1) Extracting protein from the Chinese medicinal materials scorpion, centipede and fangfeng; (2) dissolving sulfur, native copper, trichloroisocyanuric acid and FeS2 to form a sulfur-iron protein complex; (3) The reaction is catalyzed by sodium hydroxide to eventually produce sulfiron protein.

2. A method for preparing an iron-sulfur (FeS) protein for treating fungal infections according to claim 1, wherein the effective ingredients sulfur (S), protein and iron disulfide (FeS2) extracted from traditional Chinese medicine react with trichloroisocyanuric acid to generate an acidic complex, and the iron-sulfur protein complex is obtained by chemical synthesis.

3. A method for preparing iron sulfide (FeS) protein for treating fungal infections according to claim 1, wherein the temperature of the catalytic reaction is controlled between 40°C and 50°C, and the production of iron sulfide protein is promoted within this temperature range.

4. A method for preparing a sulfide iron (FeS) protein for treating fungal infections according to claim 1, wherein when the trichloroisocyanuric acid reacts with ferrous disulfide (FeS2), a sulfide iron protein with weak acidity that binds to multiple fungal cell receptors is generated.

5. A method for preparing an iron-sulfur (FeS) protein for treating fungal infections according to claim 1, wherein the generated iron-sulfur protein complex binds to the oxidative respiratory chain complex 1 and cytochrome oxidase complex 3 in the mitochondria of fungal cells, inhibiting the oxidative phosphorylation of fungal cells and thereby reducing the synthesis of ATP.

6. A method for preparing an iron-sulfur (FeS) protein for treating fungal infections according to claim 1, wherein the generated iron-sulfur-protein complex destroys the fungal cell membrane, causing it to rapidly dehydrate, denature and rupture, thereby killing the fungi.

7. A method for preparing an iron-sulfur (FeS) protein for treating fungal infections according to claim 1, wherein the iron-sulfur protein complex has a molecular formula of CI3N3O3S3, has a molecular weight of 340.695, and forms a brown precipitate by dissolving iron disulfide (FeS2).

8. A sulfur-iron-protein complex according to claim 1 for use in treating fungal infections, characterized in that By binding to multiple receptors on the surface of fungi, such as capsule proteins, cell walls, cell membranes and protein synthesis barriers, it destroys the structure and function of fungal cells, thereby achieving antifungal therapeutic effects.